CN103352009A - Bacterial with industrial oil pollution degradation function, culture medium and application thereof - Google Patents
Bacterial with industrial oil pollution degradation function, culture medium and application thereof Download PDFInfo
- Publication number
- CN103352009A CN103352009A CN2013100757661A CN201310075766A CN103352009A CN 103352009 A CN103352009 A CN 103352009A CN 2013100757661 A CN2013100757661 A CN 2013100757661A CN 201310075766 A CN201310075766 A CN 201310075766A CN 103352009 A CN103352009 A CN 103352009A
- Authority
- CN
- China
- Prior art keywords
- concentration
- greasy dirt
- mass fraction
- bacterial classification
- degradation function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to bacterial with an industrial oil pollution degradation function, wherein the bacteria is Enterobacter sp., and is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.7251. The bacterial with the industrial oil pollution degradation function easily forms zooglea, such that industrial oil pollution degradation efficiency is high, and an effect is good.
Description
Technical field
The present invention relates to microorganism field, especially relate to a kind of bacterial classification, substratum and application with industrial greasy dirt degradation function.
Background technology
Industrial greasy dirt refers to the greasy dirts such as various used oil, waste diesel or waste hydraulic oil that motor vehicle, instrument, machinery maintenance maintenance and industrial and mining enterprises etc. produce in production and operation.Industrial greasy dirt is a kind of Hazardous wastes, is difficult to natural decomposition, and quite large to the pollution of environment.According to environmental administration, in a single day the general industry greasy dirt infiltrates in the earth, and soil all can't be repaired within decades.If permeate the ground water, consequence is then hardly imaginable.Deal with improperly, one jin of used oil can pollute 1000 tons of clear water, and this is equivalent to 7 people's 1 year the water yield of drinking.Contain carcinogenic, mutagenesis, teratogenesis shape material in the used oil, very big to harm, also endanger simultaneously animal and plant growth and environment for human survival.
The greasy dirt treatment process of commonly using both at home and abroad at present has: solvent extration, thermochemistry washing method, burning method, biological process, greasy dirt comprehensive utilization etc. mainly are summarized as three class methods, i.e. physical treatment, chemical treatment and biological treatment.
Physics, chemical treatment method have landfill method, solidify burning method, chemical demulsification method, modified-the mechanical separation treatment process, high-temperature processing technology, microwave treatment technique, solvent extraction treatment process etc.Wherein, landfill and curing burning method are the common methods of the processing greasy dirt of most oil field at homes, but this method had both been wasted the valuable energy in the greasy dirt, also might cause environmental pollution.
Biological treatment is a kind of more effective contained Mud Treatment Technology, also is one of developing direction of from now on disposal of oily sludge.The cardinal principle of biological treatment is that microorganism utilizes petroleum hydrocarbon to assimilate degraded as carbon source, makes its final permineralization, changes harmless inorganic substance (CO into
2And H
2O) process.The advantage of biological treatment is: the one, and little to environmental influence, biological treatment is the reinforcement of natural process, its final product is carbonic acid gas, water and lipid acid etc., can not form secondary pollution or cause pollutent to shift; The 2nd, expense is relatively low, is about 1/4～1/3 of burning disposal expense; The 3rd, treatment effect is good, and through biochemical treatment, the pollutent residual quantity can decrease.
But traditional biological treatment also has its weak point, and treatment cycle is relatively long on the one hand, and some technology is subjected to the restriction of natural condition larger; On the other hand, from resource view, the petroleum resources in the oily sludge are not effectively utilized.Addressing these problems will be the emphasis of the industrial wipe oil microbial strains of exploitation and related products exploitation.The degraded industrial greasy dirt needs suitable microorganism, and screening efficiently degraded bacterial classification is crucial, if can obtain moving glue cenobium, and the greasy dirt of just degrading better, but at present moving glue cenobium also successfully is not used for the greasy dirt degraded.
Summary of the invention
Based on this, be necessary to provide a kind of bacterial classification, substratum and application with industrial greasy dirt degradation function.
A kind of bacterial classification with industrial greasy dirt degradation function, described bacterial classification are enterobacteria (Enterobacter sp.), and this culture presevation is at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.7251.
The moving glue cenobium that above-mentioned bacterial classification with industrial greasy dirt degradation function forms can be applicable to industrial greasy dirt degraded field.
Described have the culture presevation of industrial greasy dirt degradation function at China Committee for Culture Collection of Microorganisms common micro-organisms center, the preservation address is No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on 02 05th, 2013, and preserving number is CGMCC No.7251.
A kind of be used to cultivating above-mentioned substratum with bacterial classification of industrial greasy dirt degradation function, each component that comprises following concentration: glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and bitter salt 0.3～1g/L, solvent are water.
In a preferred embodiment, the concentration of described glucose is 2g/L, the concentration of described yeast extract is 0.5g/L, the concentration of described ammonium sulfate is 2g/L, and the concentration of described Repone K is 1.1g/L, and the concentration of described sodium-chlor is 1.1g/L, the concentration of described ferrous sulfate is 0.028g/L, the concentration of described potassium primary phosphate is 1.5g/L, and the concentration of described dipotassium hydrogen phosphate is 1.5g/L, and the concentration of described bitter salt is 0.5g/L.
A kind of liquid formulation for the degraded industrial greasy dirt, each component that comprises following concentration: above-mentioned bacterial classification 0.01～0.1g/L, glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and bitter salt 0.3～1g/L with industrial greasy dirt degradation function, solvent is water.
In a preferred embodiment, described concentration with bacterial classification of industrial greasy dirt degradation function is 0.05g/L, the concentration of described glucose is 2g/L, the concentration of described yeast extract is 0.5g/L, the concentration of described ammonium sulfate is 2g/L, the concentration of described Repone K is 1.1g/L, the concentration of described sodium-chlor is 1.1g/L, the concentration of described ferrous sulfate is 0.028g/L, the concentration of described potassium primary phosphate is 1.5g/L, the concentration of described dipotassium hydrogen phosphate is 1.5g/L, and the concentration of described bitter salt is 0.5g/L.
A kind of solid formulation for the degraded industrial greasy dirt comprises each component of following mass fraction: above-mentioned bacterial classification 0.01～0.1, glucose 1～3.5, yeast extract 0.1～1L, ammonium sulfate 1.5～3.5, Repone K 1～2.5, sodium-chlor 1～2.5, ferrous sulfate 0.01～0.05, potassium primary phosphate 1～2.5, dipotassium hydrogen phosphate 1～2.5 and bitter salt 0.3～1 with industrial greasy dirt degradation function.
In a preferred embodiment, described mass fraction with bacterial classification of industrial greasy dirt degradation function is 0.05, the mass fraction of described glucose is 2, the mass fraction of described yeast extract is 0.5, the mass fraction of described ammonium sulfate is 2, the mass fraction of described Repone K is 1.1, the mass fraction of described sodium-chlor is 1.1, the mass fraction of described ferrous sulfate is 0.028, the mass fraction of described potassium primary phosphate is 1.5, the mass fraction of described dipotassium hydrogen phosphate is 1.5, and the mass fraction of described bitter salt is 0.5.
Above-mentioned have the bacterial classification of industrial greasy dirt degradation function owing to easily form moving glue cenobium, and degraded industrial greasy dirt efficient is high.
Description of drawings
Fig. 1 is the moving glue cenobium bacterium synoptic diagram that produces after the enrichment;
Fig. 2 is visual inspection contrast before and after the degraded of mechanical workout greasy dirt, wherein, and before Fig. 2 A represents degraded, after Fig. 2 B represents degraded;
The synoptic diagram that Fig. 3 is progressively degraded for the greasy dirt that continues to add.
Embodiment
Below mainly in conjunction with the drawings and the specific embodiments bacterial classification, substratum and the application with industrial greasy dirt degradation function is further described in detail.
The bacterial classification with industrial greasy dirt degradation function of present embodiment is Colibacter E.sp(Enterobacter sp.), this culture presevation is at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.7251.
This Colibacter E.sp-Bf is that separation from the normal soil that is machined oil pollution, purifying, screening obtain.Concrete steps are as follows:
(1) separation and purification of bacterial classification: when 10 ℃ of left and right sides of external temperature in spring, take from mechanic factory workers quenching workshop section and landed the thin solum that greasy dirt polluted, will at room temperature activate 2～3 days after the mixing with soil of bacterium source with distilled water; Get 2～3g soil and add and to be equipped with in the 100mL Erlenmeyer flask of 15mL substratum (not adding the substratum of vegetables oil), the shaking table shaken overnight is cultivated under 28 ℃, 200rpm/min condition.Get elementary nutrient solution, be seeded to the cultivation of going down to posterity in the shaking flask that the 100mL substratum is housed with pipettor with 1%～2% inoculum size, be cultured to the nutrient solution in exponential growth latter stage as inoculum, with the cultivation of transferring of 1%～2% inoculum size; As stated above continuous 5 generations of enrichment culture, get last nutrient solution 100 μ L and be applied on the solid plate substratum, be coated with continuously 5～6 flat boards; Flat board is placed incubator, 28 ℃ of lower cultivations, treat single bacterium colony that dull and stereotyped upper height of formation disperses,, to slant medium, the inclined-plane placed under 28 ℃, be cultured to the formation lawn in the incubator according to bacterium colony mode of appearance picking list bacterium colony; The inclined-plane is placed 4 ℃ of refrigerator preservations; The dyed microscopy of resulting slant strains is the laggard row filter of pure bacterium.
In the present embodiment, cultivate used substratum and comprise each component of following concentration: glucose 2g/L, yeast extract 0.5g/L, ammonium sulfate 2g/L, Repone K 1.1g/L, sodium-chlor 1.1g/L, ferrous sulfate 0.028g/L, potassium primary phosphate 1.5g/L, dipotassium hydrogen phosphate 1.5g/L and bitter salt 0.5g/L, solvent are water.In other embodiments, this substratum can also be for comprising each component of following concentration: glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and bitter salt 0.3～1g/L, solvent are water.
(2) screening of bacterial classification: the picking bacterium is inoculated into the liquid nutrient medium that is added with the 20g/L vegetables oil and cultivated for 1 generation from the inclined-plane, the nutrient solution line is coated on the plate culture medium that contains vegetables oil, cultivated 2～3 days for 28 ℃, observe on the plate culture medium and obvious well-grown single bacterium colony whether occurs, repeat above-mentioned steps 3～4 times, until obtain the bacterial classification of purifying.The bacterial classification of purifying places 20mL to contain vegetables oil substratum enrichment culture, can form moving glue cenobium (being the bulk bacterium that bacterial classification flocks together and forms) as shown in Figure 1.
(3) the above-mentioned bacterial strain that optimizes is delivered Nanjing Jin Sirui Bioisystech Co., Ltd and carry out strain identification, through identifying, this bacterial strain is cultivated 18～24h and is namely formed brown opaque circular bacterium colony containing on the vegetables oil plate culture medium 28 ℃, diameter 2～4mm, be Gram-negative bacteria, thalli morphology is thicker quarter butt or spherical, diameter 1.2 μ m.Qualification result is, this bacterial strain is intestinal bacteria (Enterobacter sp), and present embodiment is with its called after E.sp-Bf(Enterobacter sp-Bf).
(4) the moving glue cenobium mechanical treated oil of degrading is dirty: with the 200mL substratum, 5mL bacterium liquid and 2mL mechanical workout greasy dirt place the 400mL Erlenmeyer flask, the about 10h of shaking table vibration under 28 ℃, 200rpm/min condition, the visual inspection greasy dirt is by degradable, as shown in Figure 2, the floating shape thing of black is greasy dirt in Fig. 2 A Erlenmeyer flask, observes that the floating shape thing of black is degraded fully among Fig. 2 B.Continue to add greasy dirt, this cenobium greasy dirt of still progressively degrading, shown in Fig. 3 A-Fig. 3 C, the floating shape thing of black progressively is degraded in the Erlenmeyer flask.Measure the COD value of its degraded front and back and find that the liquid after the degraded has reached discharging standards, feeler mechanism is that Jiangsu state surveys detection technique company limited, and the result is as shown in table 1.
COD value before and after the moving glue cenobium degraded of table 1 greasy dirt
This bacterial classification can be made liquid formulation, be used for the degraded industrial greasy dirt, concrete this liquid formulation comprises each component of following concentration: above-mentioned bacterial classification 0.01～0.1g/L, glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and bitter salt 0.3～1g/L with industrial greasy dirt degradation function, solvent is water.Further, preferred, in this liquid formulation, concentration with bacterial classification of industrial greasy dirt degradation function is 0.05g/L, the concentration of glucose is 2g/L, and the concentration of yeast extract is 0.5g/L, and the concentration of ammonium sulfate is 2g/L, the concentration of Repone K is 1.1g/L, the concentration of sodium-chlor is 1.1g/L, and the concentration of ferrous sulfate is 0.028g/L, and the concentration of potassium primary phosphate is 1.5g/L, the concentration of dipotassium hydrogen phosphate is 1.5g/L, and the concentration of bitter salt is 0.5g/L.
In addition, this bacterial classification all can also be made the solid formulation of freeze-dried type, for Powdered, comprise each component of following mass fraction: above-mentioned bacterial classification 0.01～0.1, glucose 1～3.5, yeast extract 0.1～1L, ammonium sulfate 1.5～3.5, Repone K 1～2.5, sodium-chlor 1～2.5, ferrous sulfate 0.01～0.05, potassium primary phosphate 1～2.5, dipotassium hydrogen phosphate 1～2.5 and bitter salt 0.3～1 with industrial greasy dirt degradation function.Further, preferred, in this solid formulation, mass fraction with bacterial classification of industrial greasy dirt degradation function is 0.05, the mass fraction of glucose is 2, and the mass fraction of yeast extract is 0.5, and the mass fraction of ammonium sulfate is 2, the mass fraction of Repone K is 1.1, the mass fraction of sodium-chlor is 1.1, and the mass fraction of ferrous sulfate is 0.028, and the mass fraction of potassium primary phosphate is 1.5, the mass fraction of dipotassium hydrogen phosphate is 1.5, and the mass fraction of bitter salt is 0.5.
The above embodiment has only expressed embodiments of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. bacterial classification with industrial greasy dirt degradation function, described bacterial classification is enterobacteria (Enterobacter sp.), is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.7251.
2. the moving glue cenobium that forms of the bacterial classification with industrial greasy dirt degradation function as claimed in claim 1 is in the application in industrial greasy dirt degraded field.
3. one kind is used for cultivating the substratum with bacterial classification of industrial greasy dirt degradation function claimed in claim 1, each component that comprises following concentration: glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and bitter salt 0.3～1g/L, solvent are water.
4. substratum as claimed in claim 3, it is characterized in that, the concentration of described glucose is 2g/L, and the concentration of described yeast extract is 0.5g/L, and the concentration of described ammonium sulfate is 2g/L, the concentration of described Repone K is 1.1g/L, the concentration of described sodium-chlor is 1.1g/L, and the concentration of described ferrous sulfate is 0.028g/L, and the concentration of described potassium primary phosphate is 1.5g/L, the concentration of described dipotassium hydrogen phosphate is 1.5g/L, and the concentration of described bitter salt is 0.5g/L.
5. liquid formulation that is used for the degraded industrial greasy dirt, it is characterized in that, each component that comprises following concentration: bacterial classification 0.01～0.1g/L, glucose 1～3.5g/L, yeast extract 0.1～1g/L, ammonium sulfate 1.5～3.5g/L, Repone K 1～2.5g/L, sodium-chlor 1～2.5g/L, ferrous sulfate 0.01～0.05g/L, potassium primary phosphate 1～2.5g/L, dipotassium hydrogen phosphate 1～2.5g/L and the bitter salt 0.3～1g/L with industrial greasy dirt degradation function claimed in claim 1, solvent is water.
As claimed in claim 5 for the degraded industrial greasy dirt liquid formulation, it is characterized in that, described concentration with bacterial classification of industrial greasy dirt degradation function is 0.05g/L, the concentration of described glucose is 2g/L, the concentration of described yeast extract is 0.5g/L, the concentration of described ammonium sulfate is 2g/L, the concentration of described Repone K is 1.1g/L, the concentration of described sodium-chlor is 1.1g/L, the concentration of described ferrous sulfate is 0.028g/L, the concentration of described potassium primary phosphate is 1.5g/L, and the concentration of described dipotassium hydrogen phosphate is 1.5g/L, and the concentration of described bitter salt is 0.5g/L.
7. solid formulation that is used for the degraded industrial greasy dirt, it is characterized in that, comprise each component of following mass fraction: bacterial classification 0.01～0.1, glucose 1～3.5, yeast extract 0.1～1L, ammonium sulfate 1.5～3.5, Repone K 1～2.5, sodium-chlor 1～2.5, ferrous sulfate 0.01～0.05, potassium primary phosphate 1～2.5, dipotassium hydrogen phosphate 1～2.5 and the bitter salt 0.3～1 with industrial greasy dirt degradation function claimed in claim 1.
As claimed in claim 7 for the degraded industrial greasy dirt solid formulation, it is characterized in that, described mass fraction with bacterial classification of industrial greasy dirt degradation function is 0.05, the mass fraction of described glucose is 2, the mass fraction of described yeast extract is 0.5, the mass fraction of described ammonium sulfate is 2, the mass fraction of described Repone K is 1.1, the mass fraction of described sodium-chlor is 1.1, the mass fraction of described ferrous sulfate is 0.028, the mass fraction of described potassium primary phosphate is 1.5, and the mass fraction of described dipotassium hydrogen phosphate is 1.5, and the mass fraction of described bitter salt is 0.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310075766.1A CN103352009B (en) | 2013-03-08 | 2013-03-08 | There is the bacterial classification of industrial greasy dirt degradation function, substratum and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310075766.1A CN103352009B (en) | 2013-03-08 | 2013-03-08 | There is the bacterial classification of industrial greasy dirt degradation function, substratum and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103352009A true CN103352009A (en) | 2013-10-16 |
| CN103352009B CN103352009B (en) | 2015-10-21 |
Family
ID=49308423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310075766.1A Expired - Fee Related CN103352009B (en) | 2013-03-08 | 2013-03-08 | There is the bacterial classification of industrial greasy dirt degradation function, substratum and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103352009B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709281A (en) * | 2009-12-15 | 2010-05-19 | 西北大学 | Method for producing 2,3-butanediol by fermentation of enterobacter sp.Y5 |
| CN102690763A (en) * | 2012-02-23 | 2012-09-26 | 西北大学 | Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof |
-
2013
- 2013-03-08 CN CN201310075766.1A patent/CN103352009B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709281A (en) * | 2009-12-15 | 2010-05-19 | 西北大学 | Method for producing 2,3-butanediol by fermentation of enterobacter sp.Y5 |
| CN102690763A (en) * | 2012-02-23 | 2012-09-26 | 西北大学 | Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 徐圆圆 等: "肠杆菌X-11对石油烃污染物的降解特性", 《辽宁化工》 * |
| 韩慧龙 等: "真菌．细菌修复石油污染土壤的协同作用机制研究", 《环境科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103352009B (en) | 2015-10-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nikhil et al. | Isolation, characterization and identification of diesel engine oil degrading bacteria from garage soil and comparison of their bioremediation potential | |
| CN104031870A (en) | Microbial compound bacterial agent and soil combined remediation agent prepared from microbial compound bacterial agent and application of microbial compound bacterial agent and soil combined remediation agent | |
| CN107937321B (en) | Bacillus subtilis and application thereof in degradation of polycyclic aromatic hydrocarbon pollutant phenanthrene | |
| CN103008339B (en) | Microbial remediation method for basic chromium pollution soil | |
| CN102102088B (en) | Production method of petroleum hydrocarbon pollutant-degrading bacterium | |
| CN113930365B (en) | Pseudomonas aeruginosa for degrading polycyclic aromatic hydrocarbon and application thereof | |
| CN103013850A (en) | Salt-resistant alkali-resistant Cr(VI) reduction microbe and screening method thereof | |
| CN100475950C (en) | Bacillus megaterium and use thereof | |
| CN104388328B (en) | Strain for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons and obtaining method and application thereof | |
| CN104649848A (en) | Solid bacterial fertilizer for remedying petroleum polluted saline alkali soil and preparation method of solid bacterial fertilizer | |
| CN102676424B (en) | Obligate halophile for processing high-salt oily waste liquor in petroleum industry | |
| CN104450569A (en) | Ultra-high concentration cadmium-resistant strain in mined soil and separating and screening method of ultra-high concentration cadmium-resistant strain | |
| CN104087525A (en) | Novel seawater fermentation strain generating biosurfactant | |
| CN110438033B (en) | Grease degrading bacterium, application and grease degrading method | |
| CN101972774A (en) | Microbial repair method of oil-polluted wetland | |
| CN101974466A (en) | Gordonia alkanivorans and application in oil degradation | |
| CN103045496A (en) | Preparation method of high-efficient degrading bacterium agent for phthalate ester environmental hormone | |
| CN103539493A (en) | Preparation method of polycyclic aromatic hydrocarbon contaminated soil composting conditioner | |
| CN109455891A (en) | A kind of compound bio preparation for repairing for oily sludge | |
| CN115449489A (en) | Oil reducing bacteria and composite microbial inoculum thereof, preparation method and application | |
| CN104988087A (en) | Crude-oil-contaminated soil compound microorganism remediation agent and preparation method thereof | |
| CN110079487B (en) | Bacterium for mineralizing and precipitating heavy metal lead ions and reducing hexavalent chromium ions and application thereof | |
| CN112239731B (en) | Surface active bacterial strain for degrading petroleum hydrocarbon and application thereof | |
| CN114058528A (en) | Oily pollutant degradation strain XT4, microbial agent and application | |
| CN101781624B (en) | Bacterial strain for treating oilfield produced water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151021 Termination date: 20170308 |