CN103340916A - Lindley eupatorium extract as well as preparation method and application thereof - Google Patents
Lindley eupatorium extract as well as preparation method and application thereof Download PDFInfo
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- CN103340916A CN103340916A CN2013103026121A CN201310302612A CN103340916A CN 103340916 A CN103340916 A CN 103340916A CN 2013103026121 A CN2013103026121 A CN 2013103026121A CN 201310302612 A CN201310302612 A CN 201310302612A CN 103340916 A CN103340916 A CN 103340916A
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Abstract
The invention provides a lindley eupatorium extract and a preparation method thereof. Compared with the prior art, the lindley eupatorium extract is higher in transfer rate of total flavone, and meanwhile a total flavone offer has good activity for hyperlipidemia and liver cirrhosis diseases.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract, relate to a kind of Herba Eupatorii Lindleyani extract, preparation method and application thereof in particular.
Technical background
Herba Eupatorii Lindleyani is the ground drying nest of feverfew wheel blade Herba Lycopi (Eupat/rium Lindleyanum DC.), bitter in the mouth, and property is flat, and the effect of clearing away lung-heat to relieve cough, resolving phlegm and relieving asthma, blood pressure lowering, blood fat is arranged.The contained chemical constituent of Herba Eupatorii Lindleyani herb also has alkaloids, volatile oil and coumarin, sesquiterpenoids ester etc. based on flavone compound.Flavone compound has the biological activity of series such as tangible antiulcer, spasmolytic, antiinflammatory and blood fat reducing, and the preparation of its preparation has treatment valuable and take for a long time and have no side effect to diseases such as cardiovascular and cerebrovascular vessel, arteriosclerosis, " three-hypers ".Discover that at present flavone compound and alkaloid have analgesic activity in the Herba Eupatorii Lindleyani; Flavone compound can increase pharmacological actions such as leukocyte count.Be widely used at medicine and food, for fully develop this resource comprehensively, have very important significance so from Herba Eupatorii Lindleyani, extract total flavones.
The existing method of extracting total flavones in the purification Herba Eupatorii Lindleyani, as " modern Chinese medicine is studied and put into practice " " research of lindley eupatorium herb general flavone purifying process " literary composition of the 25th the 5th phase of volume in 2011, disclosed method is: the total flavones in the ethanol extraction Herba Eupatorii Lindleyani, behind D101 macroporous resin adsorption eluting, get total flavones.The major defect of this method is: 1. purification procedures is single, can not effectively remove impurity; 2. the purity of product is not high, and impurity is more, and color and luster is bad etc.; 3. use organic reagent, do not recycle, cause the serious environmental pollution problem; 4. use high concentration ethanol in a large number, improved production cost, not environmental protection wastes energy.
Summary of the invention
For addressing the above problem, the invention provides a kind of preparation method of Herba Eupatorii Lindleyani extract, may further comprise the steps:
1) the Herba Eupatorii Lindleyani crude drug is pulverized, used the 70-90% alcohol reflux, obtain extracting solution;
2) merge extractive liquid, reclaims ethanol and is not diluted to every gram crude drug extract and is dissolved in the water of 5-10 times of weight to having the alcohol flavor, adding water, and obtains dilute aqueous solution;
3) filter dilute aqueous solution, remove solid residue, obtain clear liquid, with D101 on the clear liquid or AB-8 macroporous resin column, and use water elution macroporous resin column greater than 5 times of column volumes;
4) with the ethanol elution macroporous resin column of 20-60%, merge ethanol elution, reclaim ethanol to doing, the extract low-temperature freeze drying is prepared the Herba Eupatorii Lindleyani extract lyophilized powder.
With after the pulverizing of Herba Eupatorii Lindleyani crude drug, before alcohol reflux, also carry out soaking into Herba Eupatorii Lindleyani crude drug powder with alkaline water in step 1), the pH value that makes Herba Eupatorii Lindleyani crude drug powder is 9-11.
Before in step 1), the Herba Eupatorii Lindleyani crude drug being pulverized, also with petroleum ether or gasoline reflux, extract, Herba Eupatorii Lindleyani crude drug.
Described alkaline water is Ca/H or NaC/
3Aqueous solution.
A kind of Herba Eupatorii Lindleyani extract adopts method described above to prepare Herba Eupatorii Lindleyani extract, and wherein the general flavone content of Herba Eupatorii Lindleyani extract is higher than 50%.
The application of described Herba Eupatorii Lindleyani extract in preparation treatment atherosclerosis medicine.
The application of the Herba Eupatorii Lindleyani extract that described method prepares in preparation treatment liver cirrhosis medicine.
Useful technique effect of the present invention is: the invention provides the preparation method that a kind of Herba Eupatorii Lindleyani extracts, and the relative prior art of this method, the rate of transform of total flavones is higher.Total flavones provides thing to hyperlipidemia regulating liver-QI sclerosis disease simultaneously, has good activity.
The specific embodiment
Embodiment 1 different pre-treatment modes are to the influence of the total flavones rate of transform
1, total flavones detection method
Take by weighing the Herba Eupatorii Lindleyani ethanol extract respectively, control substance of Rutin adds an amount of dissolve with ethanol, adds 5%NaN/
21mL places 6min, adds 10%AL (N/ again
3)
31mL places 6min, adds 1m/lL
-1Na/H10mL, adding distil water is settled to scale, with blank, scans at 400-600nm wavelength place with spectrophotography behind the 15min.The result shows: Herba Eupatorii Lindleyani 70% ethanol extract is in wavelength 490nm place trap maximum, and control substance of Rutin is in 487nm place trap maximum, so select 487nm place test sample product trap.
Precision is measured control substance of Rutin stock solution 0.5,1,2,3,4,5,6mL in the 25mL volumetric flask respectively, and adding distil water adds 5%NaN/ to 10mL
21mL places 6min, adds 10%AL (N/ again
3)
31mL places 6min, adds lm/lL
-1Na/H10mL, adding distil water is settled to scale.Be blank with corresponding solution, behind the 15min, measure trap at 487nm wavelength place.Being vertical coordinate with the rutin content, is abscissa with the trap, calculates regression equation and is:
(, γ=0.9998), the rutin range of linearity is 0.10365-1.2438mg.Accurate absorption concentration is 0.2073mgmL
-1Control substance of Rutin 2mL, operation repetitive 6 times, the same standard curve of assay method, RSD are 0.435%.
Take by weighing the wild horse medical material, clay into power, cross No. six sieves (100 order), take by weighing powder 5.012g, as the censorship group, add 7/% alcohol solvent 150mL by solid-to-liquid ratio (1:30) and soak into, behind the microwave treatment 15min, backflow lixiviate 1h in 7/ ℃ the water-bath, lixiviating solution filter, centrifugal, pipette sample liquid 2.0/mL; By rutin standard curve determination method, be blank reference with reagent, under the wavelength of 487nm, survey its absorbance A, and from the relation of rutin concentration and absorbance, draw the rutin concentration C, obtain the total flavones rate of transform in the sample through converting.
The total flavones rate of transform (mg/g dry weight)=(the milliliter number is drawn in C * 25/) * V/W
Wherein, V represents extracting liquid volume (mL), and W represents sample dry weight (g).
The rate of transform that adopts the total flavones in the former medicine of ultrasonic extracting method Herba Eupatorii Lindleyani after testing is 19.33mg/g.
Take by weighing 3 groups of Herba Eupatorii Lindleyani crude drugs respectively, each 500.0g, called after 70% alcohol reflux group, alkaline pretreated group and defat pretreated group respectively;
Being treated to of 70% alcohol reflux group: with pulverizing medicinal materials, (ratio of the volume (mL) of g) ︰ 70% alcoholic solution is the ratio of 1:10 according to the Herba Eupatorii Lindleyani powder, in the Herba Eupatorii Lindleyani powder, add alcoholic solution, stir, pump in the reflux, extract, device, carry out reflux, extract, 3 times, each 60-100 minute, merge reflux extracting liquid, collect standby.
Being treated to of alkalescence pretreated group: with pulverizing medicinal materials, with 25 ℃ of saturated limewater clear liquid 250mL infiltration medical material 5h of 3 times of dilutions; Other operations are identical with the processing of 70% alcohol reflux group.
Being treated to of defat pretreated group: with the petroleum ether 1000mL reflux, extract, medical material 1h of medical material with 60-90%, volatilize the organic solvent on the medical material, with pulverizing medicinal materials, other operations are identical with the processing of alkaline pretreated group.
Detect the general flavone content of each group respectively, specifically as shown in table 1.
The different medical material preliminary treatment of table 1 mode is to the influence of the medical material total flavones rate of transform
As shown in Table 1, adopt alkaline pretreatment after, the rate of transform of total flavones significantly improves, and simultaneously, before extraction medical material is carried out the rate of transform that ungrease treatment also can effectively improve total flavones.
The separation and purification of embodiment 2 lindley eupatorium herb general flavones
1, the pretreatment before the macroporous resin column chromatography
70% alcohol reflux group, alkaline pretreated group and defat pretreated group that embodiment 1 is prepared pump in the Rotary Evaporators, are concentrated into till the no ethanol flavor, collect respectively and reclaim ethanol and concentrated clear paste.Can continue on for the extraction in the method among the embodiment 1 for the recovery ethanol of collecting; For the concentrated clear paste of collecting, (ratio of the volume (mL) of g) ︰ pure water is the ratio of 1 ︰ 10～15 according to clear paste, in clear paste, add earlier pure water, stir, fully dissolve clear paste, lysate is pumped in the sucking filtration machine again, carry out sucking filtration, collect filtering residue and filtrate respectively, the filtrate for collecting is upper prop liquid; Filtering residue for collecting is chlorophyll, and dry back is used for the additive of health food.
2, macroporous resin column chromatography is handled
Activated in suitable macroporous resin (being D101, AB-8 macroporous resin or the polyamide) chromatographic column of packing into commercially available, use earlier with the isopyknic pure water of macroporous resin column and recoil, after discharging the bubble in the macroporous resin column, pump into filter good 70% alcohol reflux group, alkaline pretreated group and defat pretreated group filtrate again, upper prop liquid adsorbs, till when in crossing the post effluent, flavone occurring, collected the post effluent.After absorption was finished, the pure water that pumps into again with 2～6 times of macroporous resin column volumes washed, and washing is entrained in the impurity of interlaminar resin, collected the scrub stream fluid respectively and had finished the macroporous resin column of absorption.For the scrub stream fluid of collecting and after crossing the merging of post effluent, carry out Aerobic Process for Treatment, back up to standard discharging.
Again in the macroporous resin column of finishing absorption, the ethanol volumetric concentration that pumps into 2～5 times of column volumes is that 5～10% alcoholic solution carries out eluting, the flow velocity of ethanol elution is 3～5 times/hour of the macroporous resin column volume finished of absorption, collects the macroporous resin column after the eluting remove impurity.For the macroporous resin column after the eluting remove impurity, the ethanol volumetric concentration that pumps into 2～6 times of column volumes again is that 20～60% alcoholic solution carries out eluting, eluting is adsorbed in the total flavones on the macroporous resin column, in the eluting effluent during no flavone till, 3～5 times/hour of the macroporous resin column volume that the flow velocity of ethanol elution is finished for absorption.Collect the macroporous resin column behind total flavones effluent and the eluting total flavones respectively.For the total flavones effluent of collecting, for the preparation of the total flavones lyophilized powder; For the macroporous resin column of eluting total flavones, can reuse after the activation.
3, preparation lyophilized powder
After macroporous resin column chromatography is finished dealing with, macroporous resin column chromatography is handled the total flavones effluent of collecting of respectively organizing and is pumped in the vacuum concentrating apparatus, be-0.06 in vacuum～-0.09MPa, temperature are under 75～90 ℃, carry out vacuum concentration respectively, in concentrated solution during no ethanol abnormal smells from the patient till, collect total flavones concentrated solution and evaporated liquor (i.e. the ethanol of Hui Shouing).For the ethanol that reclaims, can reuse after regulating its concentration; For the total flavones concentrated solution of collecting, first pre-freeze 5～6 hours in-18 ℃ refrigerator respectively, and then place freezer dryer respectively, be that-50～-60 ℃, vacuum are under the condition of 25～50Pa in temperature, dry 24～30 hours respectively, just preparing purity respectively was greater than 50% total flavones lyophilized powder; Detect respectively and respectively organize total flavones lyophilized powder content of total flavone, detection method adopts the described total flavones detection method of embodiment, detect the total flavones yield of finding defat pretreated group and alkaline pretreated group and be higher than 70% alcohol reflux group, but content of total flavone is lower than 70% alcohol reflux group in the lyophilized powder, and particular content is as shown in table 2.
The different preprocess methods of table 2 are to the influence of total flavones purification
Embodiment 3 total flavones lyophilized powders are investigated in the activity for the treatment of hyperlipidemia
Choose 60 of qualified mices, be divided into 6 groups at random, 10 every group.The Rhizoma Coptidis lyophilized powder that administration embodiment 2 prepares.70% alcohol reflux group, alkaline pretreated group and defat pretreated group are pressed crude drug 25.12,25.56,25.28gkg respectively
-1Dosage gavage the total flavones lyophilized powder; Atorvastatin calcium tablet group (positive controls) is pressed 2.20mgkg
-1Gavage; Blank group and model group such as gavage respectively at 1% hydroxy methocel (CMC) of capacity, 0.5mL10g
-1, 1 d
-1, continuous 30d.2h after last gavages medicine, each experimental group (except the blank group), mice is lumbar injection 75% egg-nog respectively, 0.5mL only, 20h takes a blood sample by eye socket, respectively organize the corresponding index of mice with serum cholesterol test kit, serum triglycerides test kit and HDL-C, LDL-C kit measurement, the result sees table 3 for details.
The level that table 3 is respectively organized mice serum cholesterol, triglyceride, HDL-C and LDL-C compares
Compare with model group:
*P ﹤ 0.05
*P ﹤ 0.01
As shown in Table 1, each group of total flavones lyophilized powder all can obviously reduce egg-nog induced mice serum cholesterol, triglyceride, LDL-C rising effect, the HDL-C level that can also obviously raise simultaneously relatively has significant difference (P ﹤ 0.05 or P ﹤ 0.01) with model group.
Choose 60 of qualified rats, be divided into 6 groups at random, 10 every group.In the normal feedstuff of mice, add cholesterol 2%, yolk powder 5%, Adeps Sus domestica 10%, methylthiouracil 0.2%, be made into high lipid food.Choose 6/ of qualified rat, be divided into 6 groups at random, every group of l/ only.The blank group is fed normal diet, feeds high lipid food for all the other 5 groups, is subjected to the reagent thing when feeding high lipid food.70% alcohol reflux group, alkaline pretreated group and defat pretreated group are pressed crude drug 25.43,25.12,25.19gkg respectively
-1Dosage ig administration, atorvastatin calcium tablet group is pressed 1.10mgkg
-1Gavage; Blank group and model group such as gavage at the 1%CMC of capacity.2 groups of administration capacity are 0.5mL100g
-1, 1 d
-1, continuous 30d.Fasting 24h after last gavages medicine through the eye socket blood sampling, measures serum cholesterol, triglyceride and the HDL-C, the LDL-C that respectively organize rat, and the result sees table 4 for details.
The level that table 4 is respectively organized rat blood serum cholesterol, triglyceride, HDL-C and LDL-C compares
Compare with model group:
*P ﹤ 0.01
As shown in Table 2, the total flavones lyophilized powder can obviously reduce experimental hyperlipemia rat serum cholesterol, triglyceride, LDL-C level, and the HDL-C level that can obviously raise simultaneously relatively has significant difference (P ﹤ 0.01) with model group.
Embodiment 3 total flavones lyophilized powders are investigated in the activity for the treatment of liver cirrhosis
60 of the SD rats in 6 months ages of Mus, male and female half and half, body constitution amount (280-25) g raises indoor temperature and remains on 24～26 ℃, and humidity maintains 50%-60%, and specially raise the chamber, and the special messenger is responsible for.60 rats are divided into 6 groups at random, i.e. normal control group, and model control group, positive controls, total flavones lyophilized powder 70% alcohol reflux group, alkaline pretreated group and defat pretreated group are pressed crude drug 25.12,25.56,25.28gkg respectively
-1Dosage ig administration, 10 every group, male and female half and half.
The normal control group gives normal saline by 3mLkg
-1Press 5mL/kg with normal saline behind the subcutaneous injection
-1Irritate stomach, 1 d
-1Model control group gives 600gL
-1Carbon tetrachloride Oleum Arachidis hypogaeae semen is pressed 3mLkg
-1Subcutaneous injection, first dose doubles, 3d injection 1 time; Behind the initial injection, press 5mLkg with normal saline
-1Irritate stomach, 1 d
-1Positive controls by preceding method injection carbon tetrachloride, is given 28mgL simultaneously
-1Colchicine is pressed 5mLkg
-1(140gkg
-1) gastric infusion, 1 d
-10.5kgL
-1Group is to 2kgL
-1Group is respectively total flavones lyophilized powder 70% alcohol reflux group, alkaline pretreated group and defat pretreated group, all injects carbon tetrachloride by preceding method, gives corresponding dosage total flavones lyophilized powder 5mL/kg simultaneously for 3 groups
-1Gastric infusion.Each organizes equal 7 weeks of successive administration of rat, and rat all opened abdomen when experiment finished under the flat state that crouches of anesthesia, went into pin with MS302 systematic survey portal pressure from portal vein, got serum then and made every biochemical analysis; Get organs such as liver, kidney, spleen, thymus, adrenal gland and claim quality, calculate following organ index: liver index=liver quality/body constitution amount * 100; Kidney index=kidney quality/body constitution amount * 1/0; Index and spleen index=spleen quality/body constitution amount * 1/0; Thymus index=thymus quality/body constitution amount * 1000; Adrenal gland's index=adrenal gland's quality/body constitution amount * 1000.It is liquid-solid fixed with Bo Shi to get leftlobe of liver, and paraffin embedding is done the pathology inspection after section, hematoxylin=Yihong dyeing and Van-Gies/nShi picric acid and the dyeing of acid fuchsin method.
Assessment content: to the observation of rat ordinary circumstance: observe the general situation of rat every day, changes in diet, 7d claims the body constitution amount 1 time.Measure the amino transaminase's of alanine aminotransferase, aspartic acid activity with reitman-frankel method, measure total serum protein, measure serum albumin levels with the bromocresol green method with biuret method, measure the content of Serum hyaluronic acid with radioimmunoassay.
The total flavones lyophilized powder sees Table 5 to the influence of rat body constitution amount, liver quality and other internal organs.The total flavones lyophilized powder sees Table 6 to the influence of rat blood serum biochemical indicator.
Table 5 carbon tetrachloride and total flavones lyophilized powder are to the influence of gland quality on rat liver,kidney,spleen, thymus and the hail
After testing for 7 weeks, each body constitution amount of organizing rat all has increase, but total flavones lyophilized powder various dose group body constitution amount is obviously organized increase than other, and comparing difference with normal control group, model control group has significance (P ﹤ 0.05-0.01).The liver quality of model control group, positive controls, 70% alcohol reflux group group significantly increases (P ﹤ 0.01) than normal control group, but alkaline pretreated group is compared the difference there was no significant difference with the defat pretreated group with the normal control group, and alkaline pretreated group and defat pretreated group obviously alleviate (P ﹤ 0.05) than model control group.Model control group liver index obviously increases than normal control group, alkalescence pretreated group and defat pretreated group and normal control group difference nonsignificance, its liver performance figure is than the obvious decline of positive controls (P ﹤ 0.05), model control group kidney index, index and spleen index increase (P ﹤ 0.05) than normal control group, thymus index and adrenal gland's index variation do not have remarkable meaning, and other four groups and normal control group, two groups of comparing differences of model control group are significantly (P ﹥ 0.05) not.
Table 6 carbon tetrachloride and total flavones lyophilized powder are to the influence of rat blood serum biochemical indicator
Compare with model group:
*P ﹤ 0.05
*P ﹤ 0.01
Alkalescence pretreated group and defat pretreated group alanine aminotransferase are than model control group, two groups of declines of positive controls, and aspartic acid amino transaminase descend than model control group, wherein 2kg/L
-1The amino transaminase of group aspartic acid is obviously than positive controls low (P ﹤ 0.05); Positive controls, alkaline pretreated group and defat pretreated group group hyaluronic acid are all than model control group low (P ﹤ 0.05); Model control group, two groups of albumin of positive controls are obviously low than normal control group, but alkaline pretreated group and defat pretreated group are unlike normal control group low (P ﹥ 0.05) and than model control group height; 70% alcohol reflux group is significantly not active for every index.
Claims (7)
1. the preparation method of a Herba Eupatorii Lindleyani extract is characterized in that: may further comprise the steps:
1) the Herba Eupatorii Lindleyani crude drug is pulverized, used the 70-90% alcohol reflux, obtain extracting solution;
2) merge extractive liquid, reclaims ethanol and is not diluted to every gram crude drug extract and is dissolved in the water of 5-10 times of weight to having the alcohol flavor, adding water, and obtains dilute aqueous solution;
3) sucking filtration is released aqueous solution, removes solid residue, obtains clear liquid, with D101 on the clear liquid or AB-8 macroporous resin column, and uses water elution macroporous resin column greater than 5 times of column volumes;
4) with the ethanol elution macroporous resin column of 20-60%, merge ethanol elution, reclaim ethanol to doing, the extract low-temperature freeze drying is prepared the Herba Eupatorii Lindleyani extract lyophilized powder.
2. the preparation method of Herba Eupatorii Lindleyani extract according to claim 1, it is characterized in that: after in step 1), the Herba Eupatorii Lindleyani crude drug being pulverized, before alcohol reflux, also carry out soaking into Herba Eupatorii Lindleyani crude drug powder with alkaline water, the pH value that makes Herba Eupatorii Lindleyani crude drug powder is 9-11.
3. the preparation method of Herba Eupatorii Lindleyani extract according to claim 2 is characterized in that: before in step 1) the Herba Eupatorii Lindleyani crude drug being pulverized, also with petroleum ether or gasoline reflux, extract, Herba Eupatorii Lindleyani crude drug.
4. the preparation method of Herba Eupatorii Lindleyani extract according to claim 2, it is characterized in that: described alkaline water is CaOH or NaCO
3Aqueous solution.
5. Herba Eupatorii Lindleyani extract is characterized in that: adopt the described method of claim 1-4 to prepare Herba Eupatorii Lindleyani extract, wherein the general flavone content of Herba Eupatorii Lindleyani extract is higher than 50%.
6. the application of the described Herba Eupatorii Lindleyani extract of claim 5 in preparation treatment atherosclerosis medicine.
7. the application of the Herba Eupatorii Lindleyani extract for preparing of the described method of employing claim 2-4 of the described Herba Eupatorii Lindleyani extract of claim 5 in preparation treatment liver cirrhosis medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106309529A (en) * | 2016-10-25 | 2017-01-11 | 南京中医药大学 | Preparation method for lindley eupatorium effective part |
| CN106943442A (en) * | 2017-04-13 | 2017-07-14 | 重庆市中药研究院 | It is a kind of to be used for antiviral Chinese medical extract and preparation method thereof |
| CN108096307A (en) * | 2014-06-04 | 2018-06-01 | 苏州大学 | Application of the eupatorium lindleynun var. trifoliolatum ethanol extract in anti-hepatic-B virus medicine is prepared |
| CN113588855A (en) * | 2021-07-16 | 2021-11-02 | 陕西中医药大学 | Quality detection method of lindley eupatorium herb |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926841A (en) * | 2010-07-28 | 2010-12-29 | 安徽科创中药天然药物研究所有限责任公司 | Total flavonoids of herba lycopi in botanical medicine for treating cardiovascular disease and preparation method thereof |
-
2013
- 2013-07-18 CN CN201310302612.1A patent/CN103340916B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926841A (en) * | 2010-07-28 | 2010-12-29 | 安徽科创中药天然药物研究所有限责任公司 | Total flavonoids of herba lycopi in botanical medicine for treating cardiovascular disease and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108096307A (en) * | 2014-06-04 | 2018-06-01 | 苏州大学 | Application of the eupatorium lindleynun var. trifoliolatum ethanol extract in anti-hepatic-B virus medicine is prepared |
| CN108096307B (en) * | 2014-06-04 | 2021-02-05 | 苏州大学 | Application of lindley eupatorium herb ethanol extract in preparing anti-hepatitis B virus medicine |
| CN106309529A (en) * | 2016-10-25 | 2017-01-11 | 南京中医药大学 | Preparation method for lindley eupatorium effective part |
| CN106309529B (en) * | 2016-10-25 | 2019-11-12 | 南京中医药大学 | A kind of preparation method of eupatorium lindleynun var. trifoliolatum active component |
| CN106943442A (en) * | 2017-04-13 | 2017-07-14 | 重庆市中药研究院 | It is a kind of to be used for antiviral Chinese medical extract and preparation method thereof |
| CN106943442B (en) * | 2017-04-13 | 2020-02-14 | 重庆市中药研究院 | Antiviral traditional Chinese medicine extract and preparation method thereof |
| CN113588855A (en) * | 2021-07-16 | 2021-11-02 | 陕西中医药大学 | Quality detection method of lindley eupatorium herb |
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