CN103323415B - A kind of inhibiting AChE detecting carbamates - Google Patents
A kind of inhibiting AChE detecting carbamates Download PDFInfo
- Publication number
- CN103323415B CN103323415B CN201310271811.0A CN201310271811A CN103323415B CN 103323415 B CN103323415 B CN 103323415B CN 201310271811 A CN201310271811 A CN 201310271811A CN 103323415 B CN103323415 B CN 103323415B
- Authority
- CN
- China
- Prior art keywords
- carbamates
- urethanes
- bromine
- acetylcholinesterase
- dithiobis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to chemical field, be specifically related to a kind of inhibiting AChE detecting carbamates.This method take acetylthiocholine iodide as substrate, dithiobis-nitrobenzoic acid (DTNB) is developer, the acetylcholinesterase of vigor >200U/g, use oxidizing urethanes, use Spectrophotometric Determination 3min absorbance changing value in time again, the suppression situation of urethanes to acetylcholinesterase can be drawn, thus measure the content of urethanes.Substrate that the present invention adopts and developer cost low, method is simple fast, and adopt oxidizing urethanes, inhibiting rate significantly improves, and method is highly sensitive, and reaction conditions gentleness is easy to control, and step is simple, and equipment needed thereby is few, is easy to operation.According to this method principle, a kind of quick testing reagent box can be developed, for the quick detection of urethanes in alcoholic beverage and fermented food.
Description
Technical field
The present invention relates to chemical field, be specifically related to a kind of inhibiting AChE detecting carbamates.
Background technology
Urethanes (Ethyl Carbamate is called for short EC) is the pollutant of fermented wine natural generation in fermentation and storage process.EC is just proved to be carcinogen as far back as nineteen forty-three.Research shows, to rodent, EC is a kind of multidigit point carcinogenic substance, can cause the diseases such as lung cancer, lymph cancer, liver cancer and cutaneum carcinoma.2007, international cancer research institute was classified as 2A class the carcinogenic toxicity of EC to the mankind.The pollution of current urethanes, is considered to the another major issue after food relaying aflatoxin.
Inhibiting AChE is most study and a kind of of relative maturity carries out the quick technology detected to organophosphorus, urethanes class agricultural chemicals.This method detects organophosphorus and urethanes class agricultural chemicals and has the advantages such as quick, easy, sensitive, low cost, has become the mainstream technology of domestic and international fast detecting pesticide residue.But, in those references, carbamate detected by it is very limited, inhibiting AChE is adopted sevin (1.0mg/kg), Furadan (0.5mg/kg), Aphox (0.3mg/kg), carbaryl (2.5mg/kg) to be detected as mentioned respectively in GBT 18630-2002 and GBT 5009-2003, also have in other documents relate to Yi Suowei, put out prestige, meta-tolyl-N-methylcarbamate (MTMC), Aldicarb etc., but all do not mention urethanes.Because carbamate is of a great variety, distinct, thus inhibiting AChE is not applicable to detect all carbamates.
In addition, because the suppression of carbamates to enzyme has selectivity, need to find suitable enzyme and corresponding substrate, developer carries out enzyme reaction.And urethanes is a kind of structure relatively simply carbamates, test shows, the urethanes of high concentration is to acetylcholinesterase unrestraint effect.Therefore, need to carry out pre-treatment to it, improve its inhibiting rate, reduce detectability, this method tool is had significant practical applications.
Summary of the invention
An object of the present invention is, provides a kind of inhibiting AChE detecting carbamates.
Another object of the present invention is, provides a kind of inhibiting AChE detecting the multiple carbamate containing amino structure such as urethanes, carbamic acid propyl ester, butyl carbamate.
Invention is achieved through the following technical solutions above-mentioned purpose.
First, invention provides a kind of inhibiting AChE of carbamates, and described enzyme is acetylcholinesterase; Adopt acetylthiocholine iodide as enzymolysis substrate, adopt dithiobis-nitrobenzoic acid as developer.
Preferably, described urethanes class is that amino ethyl amino propyl formate, butyl carbamate etc. are multiple containing amino carbamate.
Especially preferred, described urethanes, before suppressing acetylcholinesterase, is also first oxidized through the process of oxygenant.
Described oxygenant is one or more in sodium hypochlorite, calcium hypochlorite, bromine water, sodium bromate, sodium chlorate, hydrogen peroxide, bromine water-sodium hydroxide solution.
Described urethanes, after oxidation, need reduce with excessive reductive agent.
To particularly, said method comprising the steps of:
S1. pre-service: after adopting oxygenant to be oxidized the target to be measured containing urethanes, then reduce;
S2., in S1 pretreatment product, add acetylcholinesterase and acetylthiocholine iodide, react;
S3., in S2 reaction product, add dithiobis-nitrobenzoic acid and develop the color.
More specifically, comprise the following steps:
S1. pre-service: add bromine preparation in containing the target to be measured of urethanes, after oxidation 5-30min (preferably 10-25min), reduces with 5-15% sodium nitrite solution;
S2. in S1 pretreatment product, acetylcholinesterase is added, 25-40 DEG C of reaction 10-30min;
S3. in S2 reaction product, add dithiobis-nitrobenzoic acid and acetylthiocholine iodide, after colour developing, under wavelength 405-415nm, measure light absorption value; Preferred 412nm.
The amount ratio of acetylcholinesterase wherein, dithiobis-nitrobenzoic acid, acetylthiocholine iodide and bromine is 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3.
Preferably, acetylcholine ester enzyme concentration is 10-15mg/ml, and consumption is 50-150ul; Dithiobis-nitrobenzoic acid concentration is 5-10mg/ml, and consumption is 50-150ul; Acetylthiocholine iodide concentration is 5-10mg/ml, and consumption is 50-150ul; The concentration of bromine water is 3-10%, and consumption is 50-100ul.
Bromine preparation wherein and the amount ratio of sodium nitrite are volume ratio 1:1 ~ 1:5.
Preferably, the massfraction of above-mentioned adopted sodium nitrite solution is 5-15%(preferably 7-12%).
Preferably, bromine preparation is bromine water, or bromine water-sodium hydroxide solution.Alternatively, the massfraction of bromine water is 3% ~ 10%; The concentration of described bromine water is massfraction 3% ~ 10%; In bromine water-sodium hydroxide solution, the massfraction of bromine is 2% ~ 10%, and the concentration of NaOH is 0.003mol/L ~ 0.3mol/L.
Can preferred following bromine water-sodium hydroxide solution: by the NaOH of the bromine water of massfraction 3% ~ 10% and concentration 0.01 ~ 0.1mol/L, be that 5:0.2 ~ 5:1.6 mixes according to the volume ratio of bromine water and NaOH.
As wherein a kind of method of testing, the inhibiting AChE of quick detection ethyl carbamate content provided by the invention, comprises the following steps:
(1) in test tube, add 5ml urethanes standard solution (1mg/L), then add 20-200ul bromine preparation, after oxidation 5-30min, reduce with 60-600ul 5-15% sodium nitrite;
(2) 50-200ul acetylcholinesterase and 50-200ul dithiobis-nitrobenzoic acid is added, in 30-40 DEG C of water-bath 10-30min;
(3) add 50-200ul acetylthiocholine iodide, immediately in wavelength 405-415nm place 1cm cuvette colorimetric after mixing, record 3min absorbance is worth △ A over time
0and △ A
1.
The invention provides a kind of method for detecting urethanes fast.This method take acetylthiocholine iodide as substrate, dithiobis-nitrobenzoic acid (DTNB) is developer, the acetylcholinesterase of vigor >200U/g, uses oxidizing urethanes, then can measure the inhibiting rate of urethanes to enzyme fast by spectrophotometric method.
Oxidizing urethanes is first used in invention, be substrate again with acetylthiocholine iodide, dithiobis-nitrobenzoic acid (DTNB) is developer, the acetylcholinesterase of vigor >200U/g carries out enzyme reaction, absorbance can be measured fast with spectrophotometer must change, thus calculate the inhibiting rate of urethanes, concrete step is:
Described urethanes, purity more than 99%, wins biological company limited purchased from phenanthrene.
Enzyme is the acetylcholinesterase of the vigor >200U/g winning biological company limited purchased from phenanthrene.Substrate is the acetylthiocholine iodide winning biological company limited purchased from phenanthrene.
One of innovative point of the present invention is to make use of the inhibiting effect of urethanes to acetylcholinesterase.Prior art discloses and select 2,6-dichloroindophenol acetic acid esters is substrate, but, inventor finds in the acetylcholine ester enzyme level detection method of urethanes, adopts above-mentioned this substrate, has a series of defect, such as: 2, the non-common reagent of 6-dichloroindophenol acetic acid esters, not easily buys and cost is high, or needs recrystallization to be prepared.And the present invention to choose with acetylthiocholine iodide be substrate, dithiobis-nitrobenzoic acid (DTNB) is developer, has that cost is low, color stability; Except this, it is short that this method detects the used time, and namely 3min can be observed suppression situation, measures the content of urethanes.
Two of innovative point of the present invention is to adopt suitable oxygenant to be oxidized urethanes, substantially increases the susceptibility of acetylcholinesterase to urethanes oxidation product, improves the sensitivity of method.Inventor compared for multiple oxygenant, comprises sodium hypochlorite, calcium hypochlorite, bromine water, bromine water-sodium hydroxide solution, sodium bromate, sodium chlorate, hydrogen peroxide etc.Different oxygenants effect is in the method different, and wherein sodium hypochlorite, calcium hypochlorite, bromine water, bromine water-sodium hydroxide solution can significantly improve the inhibiting rate of urethanes to acetylcholinesterase, and other oxygenants then to no effect.
It should be noted that, although the embodiment of the present invention is all using urethanes as research object, but because principle of the present invention is: hypochlorite (as sodium hypochlorite, calcium hypochlorite etc.), hypobromite and the halogen such as bromine, chlorine simple substance can introduce one or two bromine/chlorine on the amino of urethanes, generate one chloro/bromo or dichloro-/bromo urethanes.As long as containing amino carbamates, all one chloro/bromo or dichloro-/bromo carbamates can be formed by the oxidation of oxygenant, thus suppress acetylcholinesterase further.Therefore, those skilled in the art can unambiguously know by inference, and the carbamate containing amino structure such as urethanes, carbamic acid propyl ester, butyl carbamate can be applicable to method of the present invention and detect.
Inhibiting AChE of the present invention compared with prior art, has the following advantages:
(1) the present invention reacts substrate acetylthiocholine iodide used, and developer dithiobis-nitrobenzoic acid (DTNB) cost is low, and method is simple and quick.
(2) the present invention adopts oxidizing urethanes, makes originally to significantly improve the urethanes inhibiting rate of this acetylcholinesterase unrestraint, and detectability significantly reduces, and can reach 1mg/L.
(3) reaction conditions gentleness of the present invention is easy to control, and step is simple, and equipment needed thereby is few, is easy to operation.
Embodiment
Below in conjunction with embodiment, the present invention is described in more detail.But these embodiments can not form limiting the scope of the invention.
The inhibiting rate addressed in embodiment, is the suppression situation of urethanes to acetylcholine esterase active, is proportionate with the concentration of urethanes.In the solution of pH7-9, acetylthiocholine iodide, by acetylcholine ester enzyme hydrolysis, generates thiocholine.Thiocholine and developer dithiobis-nitrobenzoic acid (DTNB) react, and produce yellow substance.Be worth over time by the absorbance at spectrophotometric determination 412nm place, calculate inhibiting rate, the content of urethanes can be obtained.By following formulae discovery inhibiting rate (E):
Inhibiting rate (E)=[(△ A
0-△ A
1)/△ A
0] × 100%
In formula: △ A
0--the changing value of contrast solution reaction 3min absorbance; △ A
1--the changing value of sample solution reaction 3min absorbance.
Embodiment 1:
(1) add in test tube 5mL EC standard solution (20g/L, excessive) (0.1mol/L, pH9.0 phosphate buffered saline);
(2) 50-200ul acetylcholinesterase (13mg/mL) and 50-200ul DTNB(10mg/mL is added), in 30-40 DEG C of water-bath 10-30min;
(3) add 50-200ul acetylthiocholine iodide (10mg/mL), immediately in wavelength 412nm place 1cm cuvette colorimetric after mixing, compare with the damping fluid not containing EC.Record 3min absorbance is worth △ A over time
0and △ A
1.A
0=0.3830, △ A
1=0.3910, excessive EC to acetylcholinesterase unrestraint effect.
Embodiment 2:
(1) in test tube, add 5mL EC standard solution (20g/L, excessive) (0.1mol/L, pH9.0 phosphate buffered saline), then add 20-200ul bromine preparation (bromine water-sodium hydroxide solution) and be oxidized 10-20min, reduce with 60-600ul 5-15% sodium nitrite; In the bromine water-sodium hydroxide solution adopted, the massfraction of bromine is 3%, and the concentration of NaOH is 0.001mol/L.
(2) 50-200ul acetylcholinesterase (13mg/mL) and 50-200ul DTNB(10mg/mL is added), in 30-40 DEG C of water-bath 10-30min;
(3) add 50-200ul acetylthiocholine iodide (10mg/mL), immediately in wavelength 412nm place 1cm cuvette colorimetric after mixing, compare with the damping fluid not containing EC.Record 3min absorbance is worth A over time
0and △ A
1.A
0=0.3830, △ A
1=-0.0063, through the excessive EC of bromine preparation oxidation, inhibiting rate significantly improves, and reaches 101.64 %.
Embodiment 3:
(1) in test tube, add 5ml EC standard solution (2mg/L), then add 20-200ul oxygenant (bromine preparation) and be oxidized 10-20min, reduce with 60-600ul 5-15% sodium nitrite; Bromine preparation is bromine water-sodium hydroxide solution; In the bromine water-sodium hydroxide solution adopted, the massfraction of bromine is 5%, and the concentration of NaOH is 0.002mol/L.
(2) 50-200ul acetylcholinesterase (13mg/mL) and 50-200ul DTNB(10mg/mL is added), in 30-40 DEG C of water-bath 10-30min;
(3) add 50-200ul acetylthiocholine iodide (10mg/mL), immediately in wavelength 412nm place 1cm cuvette colorimetric after mixing, compare with the damping fluid not containing EC.Record 3min absorbance is worth A over time
0and △ A
1.A
0=0.3830, △ A
1=0.23635, through the EC standard solution of oxidizing 2mg/L, inhibiting rate can reach 38.29%
Embodiment 4:
(4) (1) adds 5ml EC standard solution (1mg/L) in test tube, then adds 20-200ul oxygenant (bromine preparation) and be oxidized 10-20min, reduces with 60-600ul 5-15% sodium nitrite; Bromine preparation is bromine water-sodium hydroxide solution; Bromine water-the sodium hydroxide solution adopted, the massfraction of bromine is 10%, and the concentration of NaOH is 0.003mol/L.
(2) 50-200ul acetylcholinesterase (13mg/mL) and 50-200ul DTNB is added, in 30-40 DEG C of water-bath 10-30min;
(3) add 50-200ul acetylthiocholine iodide, immediately in wavelength 412nm place 1cm cuvette colorimetric after mixing, compare with the damping fluid not containing EC.Record 3min absorbance is worth A over time
0and △ A
1.A
0=0.3830, △ A
1=0.28065, through the EC standard solution of oxidizing 2mg/L, inhibiting rate can reach 26.72%.
Can be found out by embodiment 1-4, EC(20g/L, excessive) to acetylcholinesterase unrestraint effect, but after the oxidation of bromine preparation, not only make script to the EC of acetylcholinesterase unrestraint effect to which creating suppression, and inhibiting rate is significantly improved, 2mg/L EC inhibiting rate can reach 38.29%, 1mg/L EC inhibiting rate can reach 26.72%.If using to acetylcholine ester enzyme inhibition rate 15% as detecting standard, then use this method <1mg/L EC can be detected.This method makes the detectability of urethanes greatly reduce, and makes inhibiting AChE detect urethanes and has more actual application value containing amino carbamates.
Embodiment 5:
As embodiment 2, but have employed multiple different oxygenant and replace, detect the detection line under different condition, result is as shown in table 1.
The selection and comparison of the different oxygenant of table 1.
。
Claims (7)
1. detect an inhibiting AChE for carbamates, it is characterized in that described enzyme is acetylcholinesterase; Adopt acetylthiocholine iodide as enzymolysis substrate, adopt dithiobis-nitrobenzoic acid as developer; Wherein, described carbamates is urethanes; Described urethanes is before suppressing acetylcholinesterase, and be also first oxidized through the process of oxygenant, described oxygenant is sodium hypochlorite and/or bromine water-sodium hydroxide solution.
2. the inhibiting AChE detecting carbamates as claimed in claim 1, is characterized in that comprising the following steps:
S1. pre-service: after adopting oxygenant to be oxidized the target to be measured containing carbamates, then reduce;
S2., in S1 pretreatment product, acetylcholinesterase is added and acetylthiocholine iodide reacts;
S3., in S2 reaction product, add dithiobis-nitrobenzoic acid and develop the color.
3. the inhibiting AChE detecting carbamates as claimed in claim 1, is characterized in that comprising the following steps:
S1. pre-service: add bromine preparation in containing the target to be measured of carbamates, after oxidation 5-30min, reduces with sodium nitrite solution;
S2. in S1 pretreatment product, acetylcholinesterase is added, 30-40 DEG C of reaction 10-30min;
S3. in S2 reaction product, add acetylthiocholine iodide and dithiobis-nitrobenzoic acid, after colour developing, under wavelength 405-415nm, measure light absorption value;
The amount ratio of described acetylcholinesterase, dithiobis-nitrobenzoic acid, acetylthiocholine iodide and bromine preparation is 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3.
4. the inhibiting AChE detecting carbamates as claimed in claim 3, it is characterized in that described acetylcholine ester enzyme concentration is 10-15mg/ml, consumption is 50-150ul; Dithiobis-nitrobenzoic acid concentration is 5-10mg/ml, and consumption is 50-150ul; Acetylthiocholine iodide concentration is 5-10mg/ml, and consumption is 50-150ul; The concentration of bromine preparation is 3-10%, and consumption is 50-100ul.
5. the inhibiting AChE detecting carbamates as claimed in claim 3, it is characterized in that the massfraction of described sodium nitrite solution is 5 ~ 15%, described bromine preparation and the amount ratio of sodium nitrite are volume ratio 1:1 ~ 1:5.
6. the inhibiting AChE detecting carbamates as claimed in claim 3, is characterized in that described bromine preparation is bromine water-sodium hydroxide solution; Wherein, in bromine water-sodium hydroxide solution, the massfraction of bromine is 2% ~ 10%, and the concentration of NaOH is 0.003mol/L ~ 0.3mol/L.
7. detect a kit for carbamates, it is characterized in that containing acetylcholinesterase, acetylthiocholine iodide, dithiobis-nitrobenzoic acid and bromine preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310271811.0A CN103323415B (en) | 2013-07-01 | 2013-07-01 | A kind of inhibiting AChE detecting carbamates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310271811.0A CN103323415B (en) | 2013-07-01 | 2013-07-01 | A kind of inhibiting AChE detecting carbamates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103323415A CN103323415A (en) | 2013-09-25 |
| CN103323415B true CN103323415B (en) | 2015-08-05 |
Family
ID=49192300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310271811.0A Expired - Fee Related CN103323415B (en) | 2013-07-01 | 2013-07-01 | A kind of inhibiting AChE detecting carbamates |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103323415B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837529B (en) * | 2014-03-06 | 2016-08-24 | 北京工业大学 | A kind of detection pipe for detecting hydrogen content in water and application |
| CN104142324A (en) * | 2014-07-29 | 2014-11-12 | 华南理工大学 | Method for measuring content of ethyl urethane through visible spectrophotometry |
| CN104142378B (en) * | 2014-07-29 | 2015-12-30 | 华南理工大学 | The method of thin-layered chromatography semiquantitative determination urethanes |
| CN104266984B (en) * | 2014-10-11 | 2016-08-17 | 江南大学 | A kind of spectrophotometric method of quick detection ethyl carbamate content |
| CN106093032A (en) * | 2016-08-31 | 2016-11-09 | 孟月志 | A kind of Fast Determination of Pesticide Residue eliminates false-positive detection method |
| CN106442814A (en) * | 2016-11-09 | 2017-02-22 | 无锡艾科瑞思产品设计与研究有限公司 | Method for determining pesticide residues on grains |
| WO2019096325A1 (en) * | 2017-11-20 | 2019-05-23 | 江苏达骏生物科技有限公司 | Bisamide complex, and preparation method therefor and uses thereof |
| CN108535249B (en) * | 2018-04-25 | 2021-02-26 | 郑州贝贝生物科技有限公司 | Pesticide residue fast detection point card |
| CN108505214B (en) * | 2018-06-07 | 2020-10-02 | 南京宁高晶测生物科技有限公司 | Preparation method of nanofiber membrane for detecting content of ethyl carbamate |
| CN109187386A (en) * | 2018-08-23 | 2019-01-11 | 成都众粒生物科技有限公司 | For detecting gel and its application of Organophosphorus and carbamate pesticides class pesticide concentration |
| CN109959654A (en) * | 2019-03-25 | 2019-07-02 | 中科(大连)快检科技有限公司 | A kind of colorimetric quick detection test paper of carbamate chemicals for agriculture |
| CN113248493B (en) * | 2021-03-22 | 2022-03-25 | 广州汇标检测技术中心 | Fluorescent agent and method for rapidly and visually detecting carbamate pesticide residues |
| CN113512037B (en) * | 2021-03-22 | 2022-12-09 | 华南农业大学 | Pyridopyrazinone ionic compound and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0576667B1 (en) * | 1992-01-09 | 2001-04-04 | Charm Sciences, Inc. | Test kit and method for the determination of pesticides |
| CN1414371A (en) * | 2002-12-13 | 2003-04-30 | 江苏省农业科学院植物保护研究所 | Conventional pesticide residue quick detecting method |
| CN1687756A (en) * | 2004-12-27 | 2005-10-26 | 南京农业大学 | Kit for testing pesticide residue rapidly and use method thereof |
| CN101140236A (en) * | 2006-09-08 | 2008-03-12 | 陈福生 | Quick measuring card capable of detecting organophosphorus and carbamate chemicals residual in various agricultural products |
| CN102183396A (en) * | 2011-03-17 | 2011-09-14 | 广州绿洲生化科技有限公司 | Sample pretreatment method for detecting pesticide residue applicable to enzymatic inhibition method, and pesticide detection method and box |
| CN102353670A (en) * | 2011-06-27 | 2012-02-15 | 天津大学 | Method for detecting pesticide residues in sulfur-containing vegetables by enzyme inhibition method |
| CN102455295A (en) * | 2010-10-28 | 2012-05-16 | 中国科学院上海生命科学研究院 | Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003089599A2 (en) * | 2002-04-16 | 2003-10-30 | The Regents Of The University Of California | Ligand sensing fluorescent acetylcholinesterase for detection of organophosphate activity |
-
2013
- 2013-07-01 CN CN201310271811.0A patent/CN103323415B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0576667B1 (en) * | 1992-01-09 | 2001-04-04 | Charm Sciences, Inc. | Test kit and method for the determination of pesticides |
| CN1414371A (en) * | 2002-12-13 | 2003-04-30 | 江苏省农业科学院植物保护研究所 | Conventional pesticide residue quick detecting method |
| CN1687756A (en) * | 2004-12-27 | 2005-10-26 | 南京农业大学 | Kit for testing pesticide residue rapidly and use method thereof |
| CN101140236A (en) * | 2006-09-08 | 2008-03-12 | 陈福生 | Quick measuring card capable of detecting organophosphorus and carbamate chemicals residual in various agricultural products |
| CN102455295A (en) * | 2010-10-28 | 2012-05-16 | 中国科学院上海生命科学研究院 | Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides |
| CN102183396A (en) * | 2011-03-17 | 2011-09-14 | 广州绿洲生化科技有限公司 | Sample pretreatment method for detecting pesticide residue applicable to enzymatic inhibition method, and pesticide detection method and box |
| CN102353670A (en) * | 2011-06-27 | 2012-02-15 | 天津大学 | Method for detecting pesticide residues in sulfur-containing vegetables by enzyme inhibition method |
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国国家质量监督检验检疫总局.蔬菜中有机磷及氨基甲酸酯农药残留量的简易检测方法 酶抑制法.《GB/T 18630-2002 蔬菜中有机磷及氨基甲酸酯农药残留量的简易检测方法 酶抑制法》.2002,第215页原理,3.5还原剂. * |
| 酶抑制法快速测定农产品农药残留的研究与应用;刘永杰 等;《现代农药》;20040410;第3卷(第2期);第26页2酶抑制法原理和应用，2.1酶抑制法原理，2.2.3比色法 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103323415A (en) | 2013-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103323415B (en) | A kind of inhibiting AChE detecting carbamates | |
| Seah et al. | Identification of a serine hydrolase as a key determinant in the microbial degradation of polychlorinated biphenyls | |
| Dong et al. | Inhibitory effects of ionic liquids on the lactic dehydrogenase activity | |
| JP5255283B2 (en) | Color reagent liquid reagent and method for stabilizing the same | |
| CN104749124A (en) | Stable serum urea detection method with strong antijamming capability and reagents | |
| Qin et al. | A spore-based portable kit for on-site detection of fluoride ions | |
| Liu et al. | Highly sensitive spectrofluorimetric determination of ascorbic acid based on its enhancement effect on a mimetic enzyme-catalyzed reaction | |
| CN103063661A (en) | Tetramethylbenzidine (TMB) coloration solution and preparation method thereof | |
| Fan et al. | Evaluation of the toxicity of ionic liquids on trypsin: A mechanism study | |
| Kumar et al. | Sensitive detection of DMSO/DMF in water, human urine and blood plasma using novel 1, 8-naphthalimide-based amphiphilic spectroscopic probes | |
| Nikokavoura et al. | Evaluation of antioxidant activity of hydrophilic and lipophilic compounds in edible oils by a novel fluorimetric method | |
| CN110261625A (en) | A kind of bilirubin multiplexed detection reagents box and its application method | |
| Qin et al. | A rapid and naked-eye on-site monitoring of biogenic amines in foods spoilage | |
| Triantis et al. | Chemiluminescent studies on the antioxidant activity of amino acids | |
| Wang et al. | A dual-mode ratiometric fluorescence and smartphone-assisted colorimetric sensing platform based on bifunctional Fe, Co-CQD for glucose analysis at physiological pH | |
| Mash et al. | Effect of chlorination on the protein phosphatase inhibition activity for several microcystins | |
| Wang et al. | Study on the interaction of pentachlorophenol with urease in aqueous solution by multiple spectroscopic techniques | |
| Pitschmann et al. | Enzymatic determination of anticholinesterases using a composite carrier | |
| JP2016019497A (en) | Biogenic component measurement method and measurement composition therefor | |
| EP3467120B1 (en) | Method and reagent for quantifying cholesterol in triglyceride-rich lipoprotein | |
| Huertas-Pérez et al. | Sensitive determination of carbaryl in vegetal food and natural waters by flow-injection analysis based on the luminol chemiluminescence reaction | |
| CN102564979A (en) | Method for determining alcohol concentration by using enzyme cycling method and alcohol determination kit | |
| CN103884563B (en) | Peroxidase (POX) dyeing liquor (chemical dyeing method) | |
| CN101042353B (en) | Composition for measuring concentration of residual chlorine | |
| CN102901710A (en) | Enzyme-colorimetric method for detecting calcium peroxide in flour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160513 Address after: 5, 510410, building 58, Tong Xin Street, Sanyuanli Road, Guangzhou Avenue, Baiyun District, Guangdong Patentee after: GUANGZHOU FOOD INSTITUTE FOR DRUG CONTROL Patentee after: South China University of Technology Address before: 6, 510160 floor, No. 18 West Bay East Road, Guangzhou, Guangdong, Liwan District Patentee before: Guangzhou Liquor Detection Center Patentee before: South China University of Technology |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150805 Termination date: 20200701 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |