CN103320336A - 一株具有广谱性生物膜抑制能力的细菌 - Google Patents

一株具有广谱性生物膜抑制能力的细菌 Download PDF

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CN103320336A
CN103320336A CN2012100793729A CN201210079372A CN103320336A CN 103320336 A CN103320336 A CN 103320336A CN 2012100793729 A CN2012100793729 A CN 2012100793729A CN 201210079372 A CN201210079372 A CN 201210079372A CN 103320336 A CN103320336 A CN 103320336A
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bacteria
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于鑫
王青
邬卓颖
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Institute of Urban Environment of CAS
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Abstract

本发明涉及一株具有广谱性生物膜抑制能力的细菌。该细菌由福建省厦门市杏林湾水库水中分离得到，编号为SW9。该细菌属于成膜能力较弱的芽孢杆菌。与该细菌混合培养后，原本成膜能力较强的细菌，明显表现出成膜能力下降的现象。目前，水处理设备、医疗器械以及输水管网中出现的生物膜污染问题是一个亟待解决的技术难题，现有的控制膜污染方法以物理法和化学法为主，不仅难以从根本上抑制膜污染，容易引发二次污染，而且需要周期性地重复去除。SW9的发现，无疑为解决生物膜污染问题提供了一个新的途径。通过生物抑制的方法，不仅可以从生物膜形成初期就有效抑制膜污染，而且可以减轻生物膜二次污染问题。

Description

一株具有广谱性生物膜抑制能力的细菌
技术领域
本发明在水源水中分离出一株细菌，能够广谱性地抑制其他细菌形成生物膜，属于微生物学领域。 
背景技术
生物膜是附着在水厂构筑物池壁或管网管壁等基底材料上的细胞、多糖等胞外多聚物、有机和无机颗粒物质等组成的具有一定厚度和形态结构的膜状复合体，是给水系统等贫营养环境中微生物生长的主要方式。 
给水管壁生物膜会降低饮用水的微生物安全性，如引起管壁结垢和腐蚀，造成管网过水能力下降，影响给水系统的运行和用户端水质。此外，生物膜为包括病原微生物在内的微生物提供了可能的栖息之所。老化的生物膜脱落会造成水的色度和浊度增加，水中细菌总数上升等问题，一些水致传染疾病的发生就是由于管网中细菌的过度生长造成的。如何抑制管网生物膜的形成，已成为保障饮用水水质安全的重要问题。 
给水处理厂通过加氯消毒和管网清洗来抑制和去除生物膜，但是上述方法并不能从根本上解决生物膜的形成问题，有研究表明，即使管网中的余氯达到较高的水平，在管网末端出水中依然会出现细菌总数超标的问题。因此，通过微生物之间的相互作用，在生物膜形成初期就对其产生抑制效应，对于从根本上抑制生物膜的形成具有重要意义。 
发明内容
本发明的目的在于寻找一株具有广谱性生物膜抑制能力的细菌。 
本发明的优点在于，在生物膜形成阶段就对其产生抑制作用，有望在根本上解决管网生物膜形成的问题。 
附图说明
图1 是SW9(source water 9)对强成膜能力细菌的生物膜抑制作用示意图。图中的细菌SW9的保藏单位是中国典型培养物保藏中心，保藏单位地址是中国武汉大学，保藏日期是2012年3月4日，保藏编号是M 2012053，分类命名是Bacillus sp.SW9。 
具体实施方式
1、在福建省厦门市集美区杏林湾水源水中分离得到了多种细菌，其中包括SW9，目前该细菌保藏在中国典型培养物保藏中心，保藏单位地址是中国武汉大学，保藏日期是2012年3月4日，保藏编号是M 2012053，分类命名是Bacillus sp.SW9。 
2、对分离出的菌株进行纯培养，并测定各个菌株的成膜能力。采用结晶紫染色、酒精溶解、酶标仪测定590nm下的光密度（optical density, OD）值的方法，表征细菌的成膜能力。以此筛选出DB1等一系列强成膜能力菌株（见表1），以及成膜能力极低的SW9菌株；
3、将SW9与成膜能力较强的菌株进行混合培养，测定混合后细菌的成膜能力，并与未加SW9时相应细菌的成膜能力进行比较（见说明书附图 图1），从而得到SW9对成膜能力较强菌株的生物膜抑制效果。
 实验结果表明，当SW9菌株存在时，多数强成膜能力的菌株形成的生物膜都有显著减少。与纯培养菌株的成膜能力相比，SW9抑制了57.0%~90.2%的生物膜形成。上述结果说明Bacillus sp. SW9 具有广谱性生物膜抑制能力。 
表
Figure DEST_PATH_325765DEST_PATH_IMAGE001
 强成膜能力菌株信息
No. Strain identity Source
1 DB1 Pleomorphomonas oryzae Drinking water biofilm
2 DB2 Acidovorax ebreus Drinking water biofilm
3 DB3 Acidovoraxsp.   Drinking water biofilm
4 DB4 Bradyrhizobiumsp. Drinking water biofilm
5 SW1 Brevundimonassp. Drinking water source
6 DW1 Brevundimonassp. Drinking water
7 AC1 Brevibacillussp. Activated carbon granule
8 GS1 Acinetobactersp. Granule sludge
9 GS2 Thauerasp. Granule sludge
10 GS3 Flavobacteriumsp. Granule sludge
11 GS4 Sphingopyxissp. Granule sludge
12 GS5 Comamonassp. Granule sludge
13 SW9 Bacillus sp. Drinking water source

Claims (1)

1.一种具有广谱性生物膜抑制作用的细菌；该细菌来自福建省厦门市集美区杏林湾水源水，属于Bacillus sp.，编号为SW9 (source water 9)；SW9能够抑制多种成膜能力较强的细菌形成生物膜。
CN2012100793729A 2012-03-23 2012-03-23 一株具有广谱性生物膜抑制能力的细菌 Pending CN103320336A (zh)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101490232A (zh) * 2006-07-24 2009-07-22 金克克国际有限公司 生物膜的酶预防和控制
WO2010006235A1 (en) * 2008-07-11 2010-01-14 Novozymes A/S Bacillus velezensis strain
CN101679087A (zh) * 2007-03-23 2010-03-24 诺维信生物股份有限公司 防止和减少生物膜形成与浮游增殖

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101490232A (zh) * 2006-07-24 2009-07-22 金克克国际有限公司 生物膜的酶预防和控制
CN101679087A (zh) * 2007-03-23 2010-03-24 诺维信生物股份有限公司 防止和减少生物膜形成与浮游增殖
WO2010006235A1 (en) * 2008-07-11 2010-01-14 Novozymes A/S Bacillus velezensis strain

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MANUEL SIMOES ET AL.: "A review of current and emergent biofilm control strategies", 《LWT-FOOD SCIENCE AND TECHNOLOGY》, vol. 43, 31 December 2010 (2010-12-31) *
WU Z ET AL.: "Evidence for broad-spectrum biofilm inhibition by a bacterium Bacillus sp. SW9", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》, vol. 79, no. 5, 21 December 2012 (2012-12-21) *

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