CN103316197A - Preparation method of allium chinensis saponins extract removing nitrite - Google Patents
Preparation method of allium chinensis saponins extract removing nitrite Download PDFInfo
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- CN103316197A CN103316197A CN2013102485002A CN201310248500A CN103316197A CN 103316197 A CN103316197 A CN 103316197A CN 2013102485002 A CN2013102485002 A CN 2013102485002A CN 201310248500 A CN201310248500 A CN 201310248500A CN 103316197 A CN103316197 A CN 103316197A
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Abstract
The invention relates to a preparation method of an allium chinensis saponins extract removing nitrite. The method comprises the following steps of: crushing, digesting, filtering and concentrating allium chinensis to an extract; adding distilled water to dissolve the extract, filtering and taking a filtrate; filtering the filtrate by D101 macroporous resin, firstly, washing by distilled water and 5% ethanol in concentration, and then, eluting by ethanol with higher concentration, collecting the eluting part of ethanol with higher concentration, and condensing and vacuum-drying to obtain the allium chinensis saponins extract. The allium chinensis saponins extract has the function of eliminating activity of nitrite, wherein the nitrite removal rate reaches 99%. The extract is added to a medical carrier to prepare particles, or capsules, or troches, or oral liquids, or injections; or the extract is added into a carrier to prepare health-care beverages or foods by conventional methods. The method provided by the invention is simple and accessible and is suitable for popularization.
Description
Technical field
The present invention relates to a kind of nitrite and remove the preparation method of preparation, relate in particular to a kind of Allium chinense saponin extract preparation method of cleaning nitrite.
Background technology
The N-nitroso compound is a kind of chemical compound that animal is had strong carcinogenecity, in more than the 300 kind of nitroso compound of research, all has carcinogenesis in various degree more than 90% at present.Nitrosamine is one of the strongest chemical carcinogen known today.It can bring out the malignant tumor that the various laboratory animal Various Tissues organs that comprise the people comprise stomach, liver etc.Generally speaking, the human nitrosamine of directly from food, taking in seldom, but the precursor substance-nitrite that forms nitrosamine is ubiquitous in food, comprises in the food and various vegetable and meat that goes mouldy.Antibacterial in the human body also can generate a part of nitrites material.Therefore, cleaning nitrite is one of effective way that prevents cancer in vivo.
Allium chinense has the various biological function as a kind of medicine-food two-purpose resource with long history, is a kind of good natural product with nitrite removing effect.Therefore have huge potentiality to be exploited and using value.
Summary of the invention
The object of the invention is to by PROCESS FOR TREATMENT, obtain the saponin extract of tool cleaning nitrite take medicine-food two-purpose resource Allium chinense as raw material, utilize this extract to add again medicine or food carriers can prepare the preparation of nitrite scavenging action.
Technical scheme of the present invention is:
Adopt the alcohol steep method to extract from Allium chinense and obtain crude extract, again the Allium chinense crude extract is crossed the D101 macroporous resin, collect the ethanol elution part, concentrated final vacuum is drying to obtain the Allium chinense saponin extract.
Technical solution of the present invention realizes by following processing step:
(1) pulverizes: dry Allium chinense is crushed to 20 ~ 50 orders;
(2) the thick extraction: be 50% ~ 100% alcohol steep Allium chinense with concentration, amount of alcohol added is 5 ~ 30 times of Allium chinense material, lixiviate 0.5 ~ 3 hour, extract 1 ~ 5 time, lixiviate can be adopted ultrasonic assisted extraction or the lixiviate of water-bath circumfluence method, obtains extracting solution, filter, filtrate decompression is concentrated into extractum;
(3) the extraction essence is carried: Allium chinense extractum is dissolved in water, filters, get filtrate, upper D101 macroporous resin is used first the alcohol flushing of distilled water and 5% concentration, uses high concentration ethanol liquid eluting again, eluent middle and high concentration ethanol final concentration is 30% ~ 90%, and the amount of eluent is 3 ~ 8 times of column volume; Collect eluent, concentrated, vacuum drying namely gets the Allium chinense saponin extract.
Extract nitrite clearance rate is measured
Nitrite clearance rate: get the 10mL color comparison tube, add simulated gastric fluid 4.0mL, take out at 37 ℃ of water-bath 10min, add certain density Allium chinense saponin solution 0.8mL, add again 5 μ g/mL NaNO
2Standard solution 0.8mL takes out behind the reaction 15min in 37 ℃ of water-baths, adds immediately 0.4% p-anilinesulfonic acid. solution 0.8mL, mixing, leave standstill 5min, add 0.2% hydrochloride naphthodiamide solution 0.4mL, add water and be settled to scale, mixing leaves standstill 15min, measures light absorption value in the maximum wavelength place and is respectively A
Sample, the while replaces the blank test determination absorbance A of sodium nitrite mark liquid with distilled water
Background, with the blank test determination absorbance A of distilled water replacement saponin solution
BlankCalculate Allium chinense saponin solution to the clearance rate of nitrite by following formula.
Clearance rate E (%)=[(A
Blank+ A
Background-A
Sample)/A
Blank] * 100%
The invention has the beneficial effects as follows: preparation is simple for the Allium chinense saponin extract, is fit to very much apply.This extract cleaning nitrite effect is obvious.The Allium chinense saponin extract can join pharmaceutical carrier, mixes, and granulates, and drying is then according to the granulation agent of medicament preparation method or capsule or tablet or oral liquid or the injection of routine; Perhaps add carrier, make health beverage or health food according to conventional method.
The specific embodiment
Embodiment 1: Allium chinense is pulverized, crossed 20 mesh sieves, take by weighing 100g, add the ethanol water-bath reflux, extract, 1.5h of 600mL60%.Add for the second time 500mL60% ethanol water-bath reflux, extract, 1.0h.Add for the third time 400mL 60% ethanol water-bath reflux, extract, 0.5h.Merge three times extracting solution, Recycled ethanol, reconcentration becomes extractum.Extractum adds the 200mL dissolved in distilled water, cross leaching filtrate, D101 macroporous resin on the filtrate, adsorbed 2 hours, with distilled water and 5% ethanol elution, colourless without color and By Anthrone Sulphuric acid demonstration to eluent, use 60% ethanol elution of 3 times of column volumes instead, collect eluent, concentrated final vacuum is dry, namely gets the Allium chinense saponin extract of tool cleaning nitrite.
Embodiment 2: Allium chinense is pulverized, crossed 40 mesh sieves, take by weighing 100g, add the ethanol water-bath reflux, extract, 3.0h of 1000mL 80%.Add for the second time 800mL 80% ethanol water-bath reflux, extract, 2.0h.Add for the third time 500mL 80% ethanol water-bath reflux, extract, 1.0h.Merge three times extracting solution, Recycled ethanol, reconcentration becomes extractum.Extractum adds the 200mL dissolved in distilled water, cross leaching filtrate, D101 macroporous resin on the filtrate, adsorbed 2 hours, with distilled water and 5% ethanol elution, colourless without color and By Anthrone Sulphuric acid demonstration to eluent, use 80% ethanol elution of 8 times of column volumes instead, collect eluent, concentrated final vacuum is dry, namely gets the Allium chinense saponin extract of tool cleaning nitrite.
Embodiment 3: Allium chinense is pulverized, crossed 50 mesh sieves, take by weighing 100g, the ethanol that adds 1000mL 70% extracts 1.0h in the supersound extraction device.For the second time add 800mL 70% ethanol and in the supersound extraction device, extract 0.5h.Add for the third time 500mL 70% ethanol and in the supersound extraction device, extract 0.3h.Merge three times extracting solution, Recycled ethanol, reconcentration becomes extractum.Extractum adds the 200mL dissolved in distilled water, cross leaching filtrate, D101 macroporous resin on the filtrate, adsorbed 2 hours, with distilled water and 5% ethanol elution, colourless without color and By Anthrone Sulphuric acid demonstration to eluent, use 70% ethanol elution of 6 times of column volumes instead, collect eluent, concentrated final vacuum is dry, namely gets the Allium chinense saponin extract of tool cleaning nitrite.
Embodiment 4: Allium chinense is pulverized, crossed 20 mesh sieves, take by weighing 100g, the ethanol that adds 600mL 90% extracts 0.8h in the supersound extraction device.For the second time add 400mL 90% ethanol and in the supersound extraction device, extract 0.4h.Add for the third time 200mL 90% ethanol and in the supersound extraction device, extract 0.2h.Merge three times extracting solution, Recycled ethanol, reconcentration becomes extractum.Extractum adds the 200mL dissolved in distilled water, cross leaching filtrate, D101 macroporous resin on the filtrate, adsorbed 2 hours, with distilled water and 5% ethanol elution, colourless without color and By Anthrone Sulphuric acid demonstration to eluent, use 90% ethanol elution of 8 times of column volumes instead, collect eluent, concentrated final vacuum is dry, namely gets the Allium chinense saponin extract of tool cleaning nitrite.
Claims (1)
1. the Allium chinense saponin extract preparation method of a cleaning nitrite is characterized in that:
(1) pulverizes: dry Allium chinense is crushed to 20 ~ 50 orders;
(2) the thick extraction: be 50% ~ 100% alcohol steep Allium chinense with concentration, amount of alcohol added is 5 ~ 30 times of Allium chinense material, lixiviate 0.5 ~ 3 hour, extract 1 ~ 5 time, lixiviate can be adopted ultrasonic assisted extraction or the lixiviate of water-bath circumfluence method, obtains extracting solution, filter, filtrate decompression is concentrated into extractum;
(3) the extraction essence is carried: Allium chinense extractum is dissolved in water, filters, get filtrate, upper D101 macroporous resin is used first the alcohol flushing of distilled water and 5% concentration, uses high concentration ethanol liquid eluting again, eluent middle and high concentration ethanol final concentration is 30% ~ 90%, and the amount of eluent is 3 ~ 8 times of column volume; Collect eluent, concentrated, vacuum drying namely gets the Allium chinense saponin extract.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771421A (en) * | 2015-04-08 | 2015-07-15 | 吉林农业大学 | Application of evening primrose stems and leaves in preparing nitrite scavenging agent |
| CN112876575A (en) * | 2021-01-25 | 2021-06-01 | 徐州工程学院 | Abutilon flower stem extract, its preparation method and application in preparation of nitrite degradation scavenger |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224274A (en) * | 2008-01-10 | 2008-07-23 | 江苏吴中苏药医药开发有限责任公司 | Medicine compounds of pericarp trichosanthis, longstamen onion bulb extract and preparing method thereof |
| CN102670935A (en) * | 2012-05-23 | 2012-09-19 | 湖南师范大学 | Method for extracting total saponins from allium chinense |
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2013
- 2013-06-21 CN CN2013102485002A patent/CN103316197A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224274A (en) * | 2008-01-10 | 2008-07-23 | 江苏吴中苏药医药开发有限责任公司 | Medicine compounds of pericarp trichosanthis, longstamen onion bulb extract and preparing method thereof |
| CN102670935A (en) * | 2012-05-23 | 2012-09-19 | 湖南师范大学 | Method for extracting total saponins from allium chinense |
Non-Patent Citations (1)
| Title |
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| 刘剑青等: "响应面法优化藠头中皂苷的提取工艺研究", 《食品工业科技》, vol. 34, no. 1, 31 January 2013 (2013-01-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771421A (en) * | 2015-04-08 | 2015-07-15 | 吉林农业大学 | Application of evening primrose stems and leaves in preparing nitrite scavenging agent |
| CN112876575A (en) * | 2021-01-25 | 2021-06-01 | 徐州工程学院 | Abutilon flower stem extract, its preparation method and application in preparation of nitrite degradation scavenger |
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Application publication date: 20130925 |