CN103314972B - Applications of macrolide compound in preventing plant downy mildew - Google Patents
Applications of macrolide compound in preventing plant downy mildew Download PDFInfo
- Publication number
- CN103314972B CN103314972B CN201310277076.4A CN201310277076A CN103314972B CN 103314972 B CN103314972 B CN 103314972B CN 201310277076 A CN201310277076 A CN 201310277076A CN 103314972 B CN103314972 B CN 103314972B
- Authority
- CN
- China
- Prior art keywords
- hypothemycin
- downy mildew
- mould
- fruit
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to applications of Hypothemycin, shown in a formula (1) described in the specification, in preventing downy mildew of plant and harvested fruits. Experiments prove that the Hypothemycin can obviously inhibit the growth of peronophythora litchi and phytophthora nicotianae, and has certain inhibition activity to pythium aphanidermatum and phytophthora melonis; and in addition, the Hypothemycin has an obvious inhibition effect on the downy blight infection of the harvested litchi fruits, as well as an obvious inhibition effect on the rotting of harvested litchis.
Description
Technical field
The present invention relates to the new purposes of a kind of Macrocyclic lactone compounds Hypothemycin, relate in particular to it the plant of plant and the new purposes of the downy mildew of fruit aspect preventing and treating after adopting.
Background technology
Plant downy mildew is made a general reference conventionally by microbial plant diseases of cause of disease such as pythium spp, downy mildew, phytophthora and the white phytophthoras of Oomycete Peronosporales, produces obvious visible white shape mould (sporangium of pathogen and ascus stalk) when one of its key character is exactly serious in disease or humidity is suitable.Wherein more pandemicly at home include that melon and fruit corruption is mould, muskmelon epidemic disease is mould and Peronophythora Litchii etc.
Melon and fruit corruption mould (Pythium aphanidermatum) is a kind of important plant pathogenic fungi, cosmopolitan, often cause all kinds of crops to rot, damping off, also often cause postharvest fruit and vegetable putrid and deteriorated, bring about great losses to agricultural production and food industry.Its rape (rape damping off) of can causing harm, soybean (soybean damping off), a variety of Chinese cabbage (Chinese cabbage damping off), cauliflower (cauliflower damping off), spinach (spinach damping off), water spinach (water spinach damping off), cucumber (cucumber pythium rot), green soy bean (dish soybean damping off), beet (Sugarbeet Rhizomania), tobacco (tobacco damping off), cotton (cotton seedling damping off), broccoli (broccoli seedling damping off), Corm Fructus Foeniculi (fennel damping off), certain herbaceous plants with big flowers (certain herbaceous plants with big flowers seedling maize ear rot falls) falls, balsam pear (balsam pear damping off), seedling and the fruit of the plants such as custard squash (custard squash pythium rot), cause that seedling is dampinged off and fruit rot.Under wet condition, sick portion surface grows the cotton-shaped mould layer of flourishing white cotton.
Muskmelon epidemic disease mould (Phytophthora melonis Katsura), claim again Jue Shi epidemic disease mould P.drechsleri Tucker, its host is extensive, comprises blade, stem and the fruit of the Curcurbitaceaes such as muskmelon, balsam pear, beet, potato, tomato, watermelon, cucumber, cucurbit, pumpkin and solanaceous crops.Wherein fruit is caught an illness and is started from kiss the earth place more, the circular spot of the water stain shape of nascent dirty-green, and sick portion depression expands to rapidly the large spot of crineous afterwards, grows white short cotton wool shape mould when high humidity, produces bloom shape mould under drying condition.
Peronophythora Litchii (Peronophythora litchi Chen ex Koet a1.) is the peronophythora litchi the main pathogenic fungi of (claiming again downy mildew), is one of Major Diseases of the provinces and regions lichee such as Guangxi, Guangdong, Fujian.This sick main harm approaches ripe litchi fruits, also can endanger the sprig of Chinese olive, carpopodium, result.Their early stage produces brown or the irregular scab of grey black on pericarp surface, without limbus.When, humidity higher in temperature is larger, scab speed expanded, makes the most of or whole blackening of shell, and pulp rots, oozes out the juice of vinic acid taste, causes decayed fruit and shedding.This disease is to cause litchi fruits to adopt one of rear rotten most important disease.
Preventing and treating downy mildew major measure comprises breeding resistant variety, strengthens cultivation management, carries out prediction that disease is popular and their early stage and spray in time medicine and carry out the measures such as chemical control.The chemical agent of preventing and treating at present downy mildew has copper agent, organic sulfur agent, tpn, aliette and metalaxyl etc.
Develop immunity to drugs because traditional chemosynthesis medicament easily makes pathogen, long-term use causes drug effect to weaken, and in environment, is difficult for decomposing, and residual medicament can be lost in surrounding environment and go, and the agriculture and forestry ecosystem and human health are worked the mischief.And natural products has compared with high selectivity controlling object more, comparatively safe to people, animal and beneficial organism, environmental pollution is less, is one of emphasis of control of plant disease research in recent years.
Hypothemycin is a kind of β-resorcylic acid macrolide derivatives from the secondary metabolite of fungi, and its structure is by representing with following formula (1):
The structure of Hypothemycin (the document 1:Nair M S R.Tetrahedron Letters that was in the news first in 1980,1980,21:2011-2012), and find its its to Ustilago maydis (Ustilago maydis, Teliomycetes, Ustilaginales, Ustilago, Ustilaginaceae) and botrytis allii Munn (Botrytis allii, discomycete, wax nail Zoopagales, Botryotinia, Sclerotiniaceae) there is certain inhibition activity (document 2:Nair M S R.Tetrahedron, 1981,37:2445).Research afterwards and report mainly concentrate on antitumor activity aspect (document 3:Tanaka H.Jpn J Cancer Res, 1999,90:1139; Document 4:Schirmer A.Proc Natl Acad Sci, 2006,103:4234) and antimalarial active (document 5:Isaka M.J Org Chem, 2002,67:1561).Have not yet to see for plant (particularly adopting rear fruit) downy mildew and prevent and treat the report of aspect.
Summary of the invention
The object of the invention is to develop Hypothemycin the plant of plant and the purposes of the downy mildew of fruit aspect preventing and treating after adopting.
We are by plant downy mildew common pathogen---Peronophythora Litchii, melon and fruit corruption is mould, muskmelon epidemic disease is mould and the external inhibition activity experiment discoverable type (1) of Phytophthora nicotianae bacterium represents Hypothemycin to Peronophythora Litchii, melon and fruit corruption is mould, muskmelon epidemic disease is mould and Phytophthora nicotianae has obvious inhibitions activity, thereby realized object of the present invention.
The Hypothemycin that formula of the present invention (1) represents can be used for the plant of plant and adopts the downy mildew control of rear fruit, especially mould to Peronophythora Litchii, Phytophthora nicotianae, muskmelon epidemic disease or the rotten mould control that causes downy mildew of melon and fruit.
Formula of the present invention (1) compound can be made separately medicine and use as downy mildew resistance microbial inoculum, also can share with other downy mildew resistance microbial inoculum, to strengthen antibacterial effect or to reduce its toxic and side effect, reduce costs.The drug regimen of formula of the present invention (1) compound can be solid form, as pulvis, granule, liposome etc., its solid carrier can be nontoxic, biodegradable, hydrophily or lyophobic dust, also can be liquid form, as solution, emulsion, suspension etc., its liquid-carrier can be water, hydrophily or hydrophobic organic solvent and the mixture of various ratio in water thereof.
The preparation method of the Hypothemycin that formula of the present invention (1) represents comprises the following steps:
(1) by Paecilomyces varioti Paecilomyces sp.SC0924CGMCC No.2900 on rice solid culture medium, in dark, leave standstill and cultivate 10～30 days in 24～28 DEG C, obtain solid fermentation culture, described rice solid culture medium is made up of rice and the water of mass ratio 1:1, described Paecilomyces varioti Paecilomyces sp.SC0924CGMCC No.2900 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on February 12nd, 2009, has been disclosed in patent application 200910037639.6;
(2) by the solid fermentation culture obtaining alcohol steep 3 times, each 24h, removes ethanol water reduced pressure concentration after ethanol, uses chloroform extraction 3 times, the concentrated chloroform extract that obtains of extract;
(3) chloroform extract is carried out to silica gel column chromatography, chloroform-methanol mixed solvent with volume ratio 100:0～80:20 carries out gradient elution, with the similar stream part of thin-layer chromatography combining data detection, then the stream part after being combined with purification on normal-phase silica gel column chromatography and recrystallization is carried out separation and purification, obtain product, be defined as through structural analysis the Hypothemycin that above-mentioned formula (1) represents.
Confirm that by external inhibition activity experiment Hypothemycin can significantly suppress the growth of Peronophythora Litchii and Phytophthora nicotianae bacterium, inhibition zone width is respectively 20mm and 18mm, Hypothemycin has also shown certain inhibition activity to the rotten mould and muskmelon epidemic disease of melon and fruit is mould, and inhibition zone width is respectively 5mm and 8mm.To adopting rear litchi fruits live body bacteriostatic test, confirm that Hypothemycin has obvious inhibitory action to adopting rear litchi fruits peronospora tabacina infection by Hypothemycin.To not inoculating the litchi fruits live body bacteriostatic test of pathogen after adopting, confirm that Hypothemycin rots to have obvious inhibition to adopting rear lichee by Hypothemycin.
Embodiment
Following examples are to further illustrate of the present invention, are not limitations of the present invention.
Embodiment 1: the preparation of the compound that formula of the present invention (1) represents
Paecilomyces varioti Paecilomyces sp.SC0924CGMCC No.2900 bacterial classification is seeded in to 11.50L rice solid culture medium (rice of mass ratio 1:1 and water) upper, in dark, leaves standstill cultivation in 26 DEG C and within 22 days, obtain solid fermentation culture.By 95% alcohol steep of 1.5 times of volumes 3 times for the solid fermentation culture obtaining, each 24h, removes ethanol water reduced pressure concentration after ethanol, with equal-volume chloroform extraction 3 times, and the concentrated chloroform extract 97.50g that obtains of extract.
Chloroform extract is carried out to silica gel column chromatography, chloroform-methanol mixed solvent with volume ratio 100:0～80:20 carries out gradient elution, with the similar stream part of thin-layer chromatography combining data detection, stream part of Rf value 0.5～0.8 when benzinum-acetone volume ratio 1:1 launches on collection lamellae, after merging through purification on normal-phase silica gel column chromatography, with benzinum-acetone gradient elution of volume ratio 90:10～50:50, merge benzinum-acetone volume ratio 60:40～55:45 wash-out part and obtain colourless crystallization 7.50g by recrystallizing methanol.By spectral analysis and comparison document, determine that gained colourless crystallization is the Hypothemycin that formula (1) represents.
Its cation ESIMS m/z379[M+H]
+, 401[M+Na]
+; Anion ESIMS m/z377[M – H], 413[M+Cl]
–;
1h NMR(solvent: deuterochloroform) and
13c NMR(solvent: deuterated pyridine) data are in table 1.
The Hypothemycin that embodiment 2: embodiment 1 obtains to plant downy mildew common pathogen---Peronophythora Litchii, melon and fruit corruption is mould, muskmelon epidemic disease is mould and the external inhibition activity experiment of Phytophthora nicotianae bacterium
Experiment is with the rotten mould Pythium aphanidermatum of melon and fruit purchased from Chinese agriculture microorganism fungus kind preservation administrative center, and Peronophythora Litchii Peronophythora litchii, the mould Phytophthora melonis of muskmelon epidemic disease and Phytophthora nicotianae bacterium Phytophthora nicotianae are provided by doctor Pan Ruqian of Zi Huan institute of Agricultural University Of South China.
Measure the downy mildew resistance bacterium activity of compound H ypothemycin with filter paper agar diffusion method: by Peronophythora Litchii activate, melon and fruit corruption is mould, muskmelon epidemic disease is mould and the 4 Plants downy mildew pathogenic bacteria such as Phytophthora nicotianae bacterium are inoculated into respectively in the culture dish that PDA solid culture medium is housed, evenly for subsequent use with T-shaped spreader painting; Sample Hypothemycin to be tested is with acetone solution, be made into 20mg/mL, when experiment, experiment is got 20 μ L and is added drop-wise on filter paper (diameter is 6mm), on superclean bench, dry, make every filter paper finally contain sample 400 μ g, then filter paper is placed with in above-mentioned culture dish, 25 DEG C, after cultivating 72h under dark condition, measure antibacterial width (antibacterial width is the vertical range of filter paper edge to colony edge), three repetitions are established in experiment, make negative control with the filter paper that adds equal-volume acetone.
Result shows that Hypothemycin can significantly suppress the growth of Peronophythora Litchii and Phytophthora nicotianae bacterium, and inhibition zone width is respectively 20mm and 18mm.Hypothemycin has also shown certain inhibition activity to the rotten mould and muskmelon epidemic disease of melon and fruit is mould, and inhibition zone width is respectively 5mm and 8mm.
The Hypothemycin that embodiment 3: embodiment 1 obtains is to adopting the mould inhibition activity experiment of white epidemic disease in rear lychee storage
The Hypothemycin that test sample: embodiment 1 obtains dissolves with a small amount of ethanol, and diluting by deionized water is 100,200,300 μ g/mL, and ethanol ultimate density is volume fraction 2.5%.
Test bacterium liquid: Peronophythora Litchii is cultivated 3 days in PDA medium, adds deionized water rinsing surface spore, counts and be diluted to every mL approximately for subsequent use containing the bacterium liquid of 106 spores under microscope.
For trying lichee: Litchi Varieties " Huaihe River branch " (Litchi chinesis Sonn.cv.Huaizhi) picks up from Conghua City of Guangdong Province, adopts the rear laboratory of immediately transporting back, in 4h, start to process.Choose in the same sizely, (pericarp is most of red, and be full of cracks ditch still has part yellow to be about 8.5 one-tenth without damage by disease and insect, maturity, endocarp white or micro-red) fruit, by volume fraction 75% alcohol immersion 3 minutes, take out with purified rinse water 2 times, dry rear packet transaction.Wherein: B1(contrast) group: lichee is soaked in the drier of volume fraction 0.25% ethanol, decompression (75kPa) is processed 3 minutes, and taking-up marks 1 "+" osculum in pericarp equatorial positions with blade after drying, and inoculates bacterium liquid 10 μ L at once.B2, B3 and B4 group: lichee is soaked in the Hypothemycin solution of 100,200 and 300 μ g/mL, decompression (75kPa) is processed 3 minutes, and taking-up marks 1 "+" osculum in pericarp equatorial positions with blade after drying, and inoculates bacterium liquid 10 μ L at once.Each processing lichee is no less than 72, dries rear packing and puts into plastic casing (6, every box), and 26 ° of C preserve 3 days and 5 days, observe and record its susceptible index, and susceptible index calculation method is: 0 for infecting without obvious; 1 is slight infection; 2 be fruit for infecting area≤1/4; 3 for infecting the 1/4-1/2 that area is fruit; 4 for infecting area >=1/2, susceptible index=∑ (Infection Grades × corresponding fruit quantity)/(4 × fruit total quantity) × 100%.
Experimental result is as shown in table 2:
Table 2Hypothemycin is to adopting the inhibition activity mould to white epidemic disease in rear litchi fruits storage
The Hypothemycin that embodiment 4: embodiment 1 obtains is not to inoculating the rotten inhibition test of pathogen lichee
The Hypothemycin that test sample: embodiment 1 obtains dissolves with a small amount of ethanol, and diluting by deionized water is 100,200,300 μ g/mL, and ethanol ultimate density is volume fraction 2.5%.
For trying lichee: Litchi Varieties " Huaihe River branch " (Litchi chinesis Sonn.cv.Huaizhi) picks up from Conghua City of Guangdong Province, adopts the rear laboratory of immediately transporting back, in 4h, start to process.Choose in the same sizely, be about 8.5 one-tenth (pericarp is most of red, and be full of cracks ditch still has part yellow, endocarp white or micro-red) fruits without damage by disease and insect, maturity.Wherein: A1(contrast) group: lichee is soaked in the drier of 0.25% ethanol, decompression (75kPa) is processed 3 minutes, and taking-up is dried.A2, A3 and A4 group: lichee is soaked in the Hypothemycin solution of 100,200 and 300 μ g/mL, decompression (75kPa) is processed 3 minutes, and taking-up is dried.Each processing lichee is 36, dries rear packing and puts into plastic casing (6, every box), and 26 ° of C preserve 3 days and 5 days, observe and record its susceptible index, and susceptible index calculation method is with embodiment 3.
Experimental result is as shown in table 3:
Table 3Hypothemycin is not to inoculating the rotten inhibition of the rear lichee of adopting of pathogen
Claims (1)
1. the application in the downy mildew control of the compound representing with following formula (1) fruit the plant of plant and after adopting:
Formula (1),
Described downy mildew pathogenic bacteria are Peronophythora Litchii Peronophythora litchii, Phytophthora nicotianae bacterium Phytophthoranicotianae, the rotten mould Pythium aphanidermatum of the mould Phytophthora melonis of muskmelon epidemic disease or melon and fruit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310277076.4A CN103314972B (en) | 2013-07-03 | 2013-07-03 | Applications of macrolide compound in preventing plant downy mildew |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310277076.4A CN103314972B (en) | 2013-07-03 | 2013-07-03 | Applications of macrolide compound in preventing plant downy mildew |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103314972A CN103314972A (en) | 2013-09-25 |
| CN103314972B true CN103314972B (en) | 2014-08-06 |
Family
ID=49184259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310277076.4A Active CN103314972B (en) | 2013-07-03 | 2013-07-03 | Applications of macrolide compound in preventing plant downy mildew |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103314972B (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333206B (en) * | 2007-06-27 | 2012-12-12 | 国家海洋局第一海洋研究所 | Macrolide compounds, preparation and application thereof |
| CN102265877B (en) * | 2011-07-22 | 2013-01-23 | 中国科学院华南植物园 | Application of beta-resorcylic acid large ring lactone in controlling harmful snails |
-
2013
- 2013-07-03 CN CN201310277076.4A patent/CN103314972B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103314972A (en) | 2013-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Leelasuphakul et al. | Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit | |
| CN104762223B (en) | A kind of bacillus amyloliquefaciens BA-KA3 and its application | |
| CN102433282B (en) | Bacillus subtilis NB12, as well as culture method and application thereof | |
| Zheng et al. | Identification of volatile organic compounds for the biocontrol of postharvest litchi fruit pathogen Peronophythora litchii | |
| CN102433281B (en) | Streptomyces katrae NB20, as well as culture method and application thereof | |
| CN107083349A (en) | The white black streptomycete of one plant of disease prevention growth-promoting and its preparation and application of metabolite | |
| CN104430407B (en) | Tetramycin P is suppressing the application in Rhizoctonia solani and botrytis cinerea | |
| CN106946955B (en) | Three saccharide ester compound of fungi and its application in preparation prevention and treatment fungal diseases of plants drug | |
| Abbas et al. | Macrocyclic trichothecenes are undetectable in kudzu (Pueraria montana) plants treated with a high-producing isolate of Myrothecium verrucaria | |
| CN102199558A (en) | Culture method and applications of Pseudomonas aurantiaca | |
| CN103013860A (en) | Preparation and application of biological control bacterial strain for diseases of ginseng plant | |
| CN109844095B (en) | Aspergillus niger F22 strain having nematicidal activity against plant parasitic nematodes and use thereof | |
| CN107828665B (en) | Separation and purification method of arundina graminifolia endophytic fungi and application thereof | |
| CN103834585B (en) | The rhizosphere pseudomonas of high yield phenazine-1-carboxylic acid and azophenlyene-1-acid amides | |
| CN100381432C (en) | Pyronone antibiotic its preparation method and application | |
| CN113957003B (en) | Antifungal streptomyces hygroscopicus and application thereof | |
| de Carvalho et al. | Diversity and antimicrobial activity of culturable endophytic fungi associated with the neotropical ethnomedicinal plants Copaifera langsdorffii and Copaifera pubiflora | |
| CN106119134A (en) | Talaromyces flavus Y28 and the application in preventing and treating fruit tree putrefaction disease thereof | |
| Sharma et al. | Biocontrol potential of Streptomyces sp. M4 and salvianolic acid B produced by it against Alternaria black leaf spot | |
| CN102742585B (en) | Medium for simple and quick separation of phytophthora melonis | |
| Steyaert et al. | Methods for the evaluation of the bioactivity and biocontrol potential of species of Trichoderma | |
| CN104450835A (en) | Preparation method of new compound | |
| CN104962501B (en) | A kind of preparation and application of the bacterial strain, antagonist of anti-vegetable and fruit gray mold | |
| CN103314972B (en) | Applications of macrolide compound in preventing plant downy mildew | |
| CN102763662B (en) | Medium for separating Peronophythora Litchi Chen ex Ko et al |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |