CN103305416A - Adjustable magnetic field based tumor cell immunoscreening system - Google Patents
Adjustable magnetic field based tumor cell immunoscreening system Download PDFInfo
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- CN103305416A CN103305416A CN2013101862881A CN201310186288A CN103305416A CN 103305416 A CN103305416 A CN 103305416A CN 2013101862881 A CN2013101862881 A CN 2013101862881A CN 201310186288 A CN201310186288 A CN 201310186288A CN 103305416 A CN103305416 A CN 103305416A
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Abstract
The invention discloses an adjustable magnetic field based tumor cell immunoscreening system which comprises a microchip and a permanent magnet, wherein the microchip comprises a slice and a glass slide, and a closed microfluid channel is formed between the slice and the glass slide. The permanent magnet is arranged outside the microfluid channel and is close to the glass slide. A distance adjusting mechanism is arranged between the permanent magnet and the glass slide to adjust the magnetic field strength and gradient to the microfluid channel by the permanent magnet. The system has the advantages that appropriate magnetic field strength and gradient can be adjusted according to tumor cells in different types to collect the tumor cells on the glass slide, and the required magnetic field strength and gradient can be further adjusted and collected according to different speeds of blood flowing through the microfluid channel, so that the optimal capture effect can be obtained.
Description
Technical field
The present invention relates to a kind of immune tumour cell screening system, especially relate to a kind of tumour cell immunoscreening system based on controlling magnetic field.
Background technology
The blood testing biochip is the diagnostic tool of future generation that detects rare cell in the blood relevant with major disease, and rare cell comprises cell with specified protein mark of circulating tumor cell (CTC), pernicious stem cell and the pathology of circulation in blood sample etc.Relevant studies confirm that, the circulating tumor cell quantity that occurs in patient's the blood sample and patient's early diagnosis and survival rate have very strong dependency.Therefore, be the key that improves tumor disease early discovery rate and personalized treatment to the rare cell determination and analysis of patient's blood sample.
The applying date that is all the applicant's application is on November 20th, 2012, the patent No. is that 201210477243.5 Chinese invention patent application file discloses " a kind of biochip for screening blood rare cell ", its structure is as follows: comprise the reeded thin slice of tool and slide glass, groove on the thin slice is close on the described slide glass towards slide glass and with thin slice, makes and forms an airtight microfluidic channel between thin slice and the slide glass; Offer the fluid hole and the inlet opening that are communicated with described microfluidic channel on the described thin slice.
At first in blood sample, put into magnetic nano-particle, the magnetic nano-particle surface is with antibody, be adsorbed on the target cell by immune response, then the blood sample that rare cell has been labeled the microfluidic channel of flowing through, the rare cell that is attached with magnetic nano-particle in the blood sample is placed on the fixedly gradient introduction by magnetic field that the magnetic substance below the biochip sends, and then be collected on the slide glass, since between thin slice and the slide glass for removably connecting, after finishing Deng collecting work, the user can be easily with slide glass and slice separates, take out slide glass, the rare cell on the slide glass is carried out subsequent analysis.
The applying date is on October 15th, 2012, the patent No. is that 201210389753.7 Chinese invention patent application file discloses " separometer of rare cell in a kind of blood ", its structure is as follows: comprise storer and pump, microchip, microchip comprises the reeded thin slice of tool and slide glass, and thin slice is arranged on and forms a microfluidic channel on the slide glass; This microfluidic channel is connected between this storer and this pump, allows this blood sample from the memory stream to the pump; A plurality of magnet are positioned at this slide glass bottom, form the fixedly Distribution of Magnetic Field of gradient along the length of slide glass, and slide glass is used for gathering the rare cell that microchip is caught.
The magnetic substance that discloses in above-mentioned two patents is arranged on the bottom of slide glass, magnetic substance is to the blood magnetic force constant in the microfluidic channel, but the antigen molecule number of different sorts tumor cell surface is different, and the nano particle number that adsorbs is also different, and required magneticstrength is also different.2, blood flow is subjected to many-sided factor affecting and difference through the speed of microfluidic channel, such as the suction force of pump, and thicknesses such as pipe connecting etc., it is also different that slide glass gathers the required magneticstrength of rare cell.
Summary of the invention
Technical problem to be solved by this invention provides a kind of based on magneticstrength and the adjustable tumour cell immunoscreening of gradient system, regulate different types of tumour cell that suitable magneticstrength and gradient gather, simultaneously can regulate magneticstrength and the gradient that will reach through the friction-motion speed of microfluidic channel according to blood flow, to reach best capture effect.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of tumour cell immunoscreening system based on controlling magnetic field, comprise microchip and permanent magnet, described microchip comprises thin slice and slide glass, forms an airtight microfluidic channel between thin slice and the slide glass; Described permanent magnet is arranged on outside the microfluidic channel, described permanent magnet is near described slide glass, be provided with distance adjusting mechanism between described permanent magnet and the described slide glass, regulate permanent magnetic field to magneticstrength and the gradient of microfluidic channel inside by described distance adjusting mechanism.By the distance adjustment between permanent magnet and the wave carrier piece, mainly regulate permanent magnet to the magneticstrength of microfluidic channel inside, next is because of the capable property of the non-line feature of Distribution of Magnetic Field, so that field gradient also can change thereupon.
Described microchip is installed in the housing, described housing is fixedly mounted on the permanent seat, described distance adjusting mechanism comprises knob assembly, be arranged on the cavity on the permanent seat and be used for installing the box body of permanent magnet, described box body is positioned at described cavity, the height of described cavity is greater than the height of box body, under the effect of knob assembly, box body is mobile in cavity, make box body near or away from described housing.
Described cavity is surrounded by the framework on the permanent seat and housing, described framework is opened wide on one side near the housing place, described framework is provided with through hole on one side away from the housing place, described knob assembly comprises knob and bolt, the position of described knob is fixed, and an end and the knob of bolt are spirally connected, and bolt passes described through hole, the other end and the box body of bolt are fixed, and rotate described knob and make box body mobile in described cavity by bolt.
Also comprise stepper-motor, main drive shaft and turning up frame, described a plurality of permanent seats are installed in the described turning up frame, and described stepper-motor drives described main drive shaft, and described main drive shaft drives described turning up frame and carries out rotary movement.
Described stepper-motor is installed on the anchor, described stepper-motor is connected with described main drive shaft by described driving gear set, described driving gear set comprises transition gear and driving toothed gear, the output shaft of described stepper-motor and described transition gear are fixed, described transition gear and the engagement of described driving toothed gear, described driving toothed gear and described main drive shaft are fixed.
Described anchor comprises left anchor and the right anchor that separates from the turning up frame both sides, be provided with left bracket on the described left anchor, described main drive shaft passes described left bracket, the left end of described main drive shaft and described turning up frame is fixed, be provided with right axle seat on the described right anchor, be provided with driven shaft between the right-hand member of described turning up frame and the right axle seat.
Described permanent magnet has three, and described three block permanent magnets are placed in the described box body side by side.
Compared with prior art, advantage of the present invention is to put into magnetic nano-particle in blood sample, the magnetic nano-particle surface is with antibody, be adsorbed on the target cell by immune response, when the blood sample that has been labeled when rare cell is flowed through microfluidic channel, the rare cell that is attached with magnetic nano-particle in the blood sample is placed on the introduction by magnetic field that the magnetic substance below the biochip sends, thereby is collected on the slide glass.Be provided with distance adjusting mechanism between permanent magnet and the slide glass, regulate permanent magnetic field to magneticstrength and the gradient of microfluidic channel inside by distance adjusting mechanism, regulate suitable magneticstrength and gradient collects tumour cell on the slide glass according to different types of tumour cell, also can regulate through the friction-motion speed of microfluidic channel according to blood flow and gather required magneticstrength and gradient, to reach best capture effect.
Description of drawings
Fig. 1 is structure sectional view of the present invention;
Fig. 2 is microchip of the present invention and the figure that cooperates of housing;
Fig. 3 is exploded view of the present invention;
Fig. 4 is the structure iron of anchor of the present invention;
Fig. 5 is A place enlarged view among Fig. 1.
Embodiment
Embodiment is described in further detail the present invention below in conjunction with accompanying drawing.
A kind of tumour cell immunoscreening system based on controlling magnetic field comprises microchip 1 and permanent magnet 2, and microchip 1 comprises thin slice 3 and slide glass 4, forms an airtight microfluidic channel between thin slice 3 and the slide glass 4; Permanent magnet 2 is arranged on outside the microfluidic channel, and permanent magnet 2 is provided with distance adjusting mechanism near slide glass 4 between permanent magnet 2 and the slide glass 4, regulates magneticstrength and the gradient of 2 pairs of microfluidic channel inside of permanent magnetic field by distance adjusting mechanism.
Microchip 1 is installed in the housing 5, housing 5 is fixedly mounted on the permanent seat 6, distance adjusting mechanism comprises knob assembly 7, be arranged on the cavity 8 on the permanent seat 6 and be used for installing the box body 9 of permanent magnet 2, box body 9 is positioned at cavity 10, the height of cavity 10 is greater than the height of box body 9, under the effect of knob assembly 7, box body 9 is in cavity 10 interior movements, make box body 9 near or away from shell 5 bodies.
Also comprise stepper-motor 12, main drive shaft 13 and turning up frame 14, a plurality of permanent seats 6 are installed in the turning up frame 14, and stepper-motor 12 drives main drive shaft 13, and main drive shaft 13 drives turning up frame 14 and carries out rotary movement.
Stepper-motor 12 is installed on the anchor 15, stepper-motor 12 is connected with main drive shaft 13 by driving gear set, driving gear set comprises transition gear 16 and driving toothed gear 17, the output shaft of stepper-motor 12 and transition gear 16 are fixing, transition gear 16 and driving toothed gear 17 engagements, driving toothed gear 17 is fixing with main drive shaft 13.
Permanent magnet 2 has three, and three block permanent magnets 2 are placed in the box body 9 side by side.
Claims (7)
1. the tumour cell immunoscreening system based on controlling magnetic field is characterized in that comprising microchip and permanent magnet, and described microchip comprises thin slice and slide glass, forms an airtight microfluidic channel between thin slice and the slide glass; Described permanent magnet is arranged on outside the microfluidic channel, described permanent magnet is near described slide glass, be provided with distance adjusting mechanism between described permanent magnet and the described slide glass, regulate permanent magnetic field to magneticstrength and the gradient of microfluidic channel inside by described distance adjusting mechanism.
2. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 1, it is characterized in that described microchip is installed in the housing, described housing is fixedly mounted on the permanent seat, described distance adjusting mechanism comprises knob assembly, be arranged on the cavity on the permanent seat and be used for installing the box body of permanent magnet, described box body is positioned at described cavity, the height of described cavity is greater than the height of box body, under the effect of knob assembly, box body is mobile in cavity, make box body near or away from described housing.
3. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 2, it is characterized in that described cavity is surrounded by the framework on the permanent seat and housing, described framework is opened wide on one side near the housing place, described framework is provided with through hole on one side away from the housing place, described knob assembly comprises knob and bolt, the position of described knob is fixed, one end and the knob of bolt are spirally connected, bolt passes described through hole, the other end and the box body of bolt are fixed, and rotate described knob and make box body mobile in described cavity by bolt.
4. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 3, characterized by further comprising stepper-motor, main drive shaft and turning up frame, described a plurality of permanent seat is installed in the described turning up frame, described stepper-motor drives described main drive shaft, and described main drive shaft drives described turning up frame and carries out rotary movement.
5. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 4, it is characterized in that described stepper-motor is installed on the anchor, described stepper-motor is connected with described main drive shaft by described driving gear set, described driving gear set comprises transition gear and driving toothed gear, the output shaft of described stepper-motor and described transition gear are fixed, described transition gear and the engagement of described driving toothed gear, described driving toothed gear and described main drive shaft are fixed.
6. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 5, it is characterized in that described anchor comprises left anchor and the right anchor that separates from the turning up frame both sides, be provided with left bracket on the described left anchor, described main drive shaft passes described left bracket, the left end of described main drive shaft and described turning up frame is fixed, be provided with right axle seat on the described right anchor, be provided with driven shaft between the right-hand member of described turning up frame and the right axle seat.
7. a kind of tumour cell immunoscreening system based on controlling magnetic field according to claim 1 is characterized in that described permanent magnet has three, and described three block permanent magnets are placed in the described box body side by side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101862881A CN103305416A (en) | 2013-05-17 | 2013-05-17 | Adjustable magnetic field based tumor cell immunoscreening system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101862881A CN103305416A (en) | 2013-05-17 | 2013-05-17 | Adjustable magnetic field based tumor cell immunoscreening system |
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| CN103305416A true CN103305416A (en) | 2013-09-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2013101862881A Pending CN103305416A (en) | 2013-05-17 | 2013-05-17 | Adjustable magnetic field based tumor cell immunoscreening system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041814A (en) * | 2006-12-29 | 2007-09-26 | 上海师范大学 | Magnetic nano cell automatic sorting device and uses thereof |
| CN102911864A (en) * | 2012-10-15 | 2013-02-06 | 宁波美晶医疗技术有限公司 | Separator for rare cells in blood |
| CN102965276A (en) * | 2012-11-20 | 2013-03-13 | 宁波美晶医疗技术有限公司 | Biochip for screening rare cells in blood |
-
2013
- 2013-05-17 CN CN2013101862881A patent/CN103305416A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041814A (en) * | 2006-12-29 | 2007-09-26 | 上海师范大学 | Magnetic nano cell automatic sorting device and uses thereof |
| CN102911864A (en) * | 2012-10-15 | 2013-02-06 | 宁波美晶医疗技术有限公司 | Separator for rare cells in blood |
| CN102965276A (en) * | 2012-11-20 | 2013-03-13 | 宁波美晶医疗技术有限公司 | Biochip for screening rare cells in blood |
Non-Patent Citations (3)
| Title |
|---|
| HOSHINO ET AL.: "Microchip-based immunomagnetic detection of circulating tumor cells", 《LAB ON A CHIP》 * |
| HUANG ET AL.: "Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system", 《BIOMED MICRODEVICES》 * |
| 官月平等: "生物磁性分离研究进展-磁性分离装置和生物技术应用", 《化工学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
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Application publication date: 20130918 |