CN103301085A - Aquatic animal medicine feeding capsule and preparation method thereof - Google Patents
Aquatic animal medicine feeding capsule and preparation method thereof Download PDFInfo
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- CN103301085A CN103301085A CN2013102202249A CN201310220224A CN103301085A CN 103301085 A CN103301085 A CN 103301085A CN 2013102202249 A CN2013102202249 A CN 2013102202249A CN 201310220224 A CN201310220224 A CN 201310220224A CN 103301085 A CN103301085 A CN 103301085A
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Abstract
The invention discloses an aquatic animal medicine feeding capsule and a preparation method thereof. The preparation method comprises the following steps of: uniformly mixing medicines to be fed and fish feeds, subsequently packaging the mixture into gastric-soluble hollow capsules to form medicine capsules, further covering a layer of agar on the surfaces of the medicine capsules, and spraying a layer of dimethyl-beta-dimethylpropiothetin (DMPT) on the surface of the agar, and airing to obtain the aquatic animal medicine feeding capsules. The aquatic animal medicine feeding capsule disclosed by the invention can be directly swallowed by fishes, so that experimental steps of ordinary drenching and the like are avoided, stress harm and operation harm to fishes are reduced, the phenomenon of return vomit is reduced, and the quantitative feeding of the medicines can be accurately carried out.
Description
Technical field
The invention belongs to aquaculture field, be specifically related to a kind of aquatic animal medicine capsule and preparation method thereof of throwing something and feeding.
Background technology:
In research process such as drug residue metabolic rule, it is experimental procedure the most rambunctious that the accurate timing of medicine is is quantitatively thrown something and fed always, the success or failure that often influence last experimental data and determine to test.
Traditional feeding method has two kinds: a kind of is that medicine directly gavages, and another kind is that the direct mixed fodder of medicine is thrown something and fed.Medicine directly gavage generally be with the medicine of throwing something and feeding with dissolved in distilled water or be configured to suspension, directly fish is gavaged; It then is that the direct mixed dust formulation feedstuff of medicated powder is made pill that the direct mixed fodder of medicine is thrown something and fed, and perhaps immerses feedstuff with outstanding turbid medicinal liquid and makes bait feeding.Occur a kind of improved method in recent years and then be in pharmaceutical chemistry, adding pigment or other coloring agent, be used for judging the situation of telling of returning that medicine raises.
And in the process that test is thrown something and fed, find that there are a lot of problems in these methods, cause the inaccurate or whole experiment of last experimental result to finish.One, suspension in gavaging process easily precipitation cause and gavage the content instability, operation is not easy, and occurs easily returning telling phenomenon, returns to be difficult to observe after telling.Two, directly gavage the throat stomach function regulating of wounding fish easily by stabbing of throwing something and feeding and cause testing and occur behind the fish medicine feed deadly easily, and the fish body surface is caused damage easily when gavaging by force, be difficult to repeatedly oral perfusion.Three, add pigment to throwing something and feeding medicine and have or not influence temporarily not have the correlational study report at the fish internal metabolism, and add that the mouthfeel to feedstuff has certain influence behind the pigment, be easier to occur returning and tell phenomenon.Therefore, develop a kind of novel medicament and throw something and feed that research has very important meaning to mode for medicine is residual etc. to the medicine of aquatic animal.
Summary of the invention:
The purpose of this invention is to provide a kind of pharmaceutical chemistry that can effectively solve and return the problems such as medicament contg is inaccurate of telling and throw something and feed, for researchs such as pharmacokinetics provide the aquatic animal medicine of the condition capsule and preparation method thereof of throwing something and feeding.
The aquatic animal medicine of the present invention capsule of throwing something and feeding prepares by the following method, and this method may further comprise the steps:
Behind the medicine and fish feed mix homogeneously that needs are thrown something and fed, encapsulate with the gastric solubleness Capsules, become medicament capsule after the encapsulation, again at medicament capsule surface coverage one deck agar, after agar surface is sprayed one deck dimethyl-β-DMPT (DMPT), dry standby again.
Described gastric solubleness Capsules is gastric solubleness 4# Capsules.
The aquatic animal medicine of the present invention capsule of throwing something and feeding can directly be swallowed by Fish, thereby need not conventional experimental procedure such as gavage, reduce to Fish stress injure and operate injury, reduce to return and tell phenomenon, can more accurately carry out medicine and quantitatively throw something and feed.
The specific embodiment:
Following examples are to further specify of the present invention, rather than limitation of the present invention.
Embodiment 1:
Carry out three kinds of different pharmaceutical feeding experiments at the indoor culture cement pit, respectively to three groups of tilapia (150g ± 20g) carry out the former powder of drug florfenicol (to herd available from Zhe Jiangkang that specification is close, throwing something and feeding or gavage experiment content 90%), all choose the healthy tilapia of 10 tails for every group, other establishes one group of blank.First group: 2ml distilled water, an amount of common fish powder feedstuff and the former powder mix homogeneously of 18mg drug florfenicol are configured to suspension, directly fish are once gavaged then, every fish gavages the 0.2ml suspension; Second group: common fish powder feedstuff and the former powder of drug florfenicol directly are mixed and made into pill in mass ratio 36:1 ratio, and every ball contains common fish powder feedstuff 65mg approximately, and the former powder 1.8mg of drug florfenicol throws something and feeds tilapia once naturally; The 3rd group: with common fish powder feedstuff and the former powder of florfenicol press mass ratio 36:1 mixed evenly after, encapsulate with gastric solubleness 4# Capsules, become medicament capsule after the encapsulation, each capsule contains common fish powder feedstuff 65mg and the former powder 1.8mg of drug florfenicol approximately, before using medicament capsule is leached deployed plain agar, make medicament capsule surface coverage one deck agar, after agar surface is sprayed one deck dimethyl-β-DMPT (DMPT), dry standbyly then, obtain the aquatic animal medicine capsule of throwing something and feeding.Producing the pure common fish powder feedstuff capsule that does not contain medicine in addition, to do domestication standby, and its manufacturing step and the above-mentioned aquatic animal medicine capsule of throwing something and feeding is identical, does not just contain the former powder of medicine florfenicol.Before throwing something and feeding, make daily feedstuff feeding with the pure common fish powder feedstuff capsule that does not contain medicine earlier, every day, every fish was thrown something and fed 3-5, tamed 3-7 days.Can directly use the aquatic animal medicine capsule of throwing something and feeding to throw something and feed after the domestication.
Blank group does not have the medicine of throwing something and feeding, the routine feedstuff of throwing something and feeding.
Experimental result shows, first group of tilapia surpasses 50% and returning and tell in various degree occur, gavages dosage and is difficult to quantitatively, and the tilapia that quilt was gavaged reason such as is wounded by stabbing and just occurred 30% death in second day owing to throat; Whether fully second group of pharmaceutical chemistry owing to directly be immersed in the drug moiety that causes in the water and run off and be difficult to estimation, pharmaceutical chemistry occurs and situation such as chewed and bite into pieces in the process of throwing something and feeding when tilapia eats pharmaceutical chemistry, also be difficult to judge tilapia clothes experiment required dosage down; Throw something and feed respond wellly for the 3rd group, pharmaceutical chemistry can only whole be swallowed, medicine can breakage or loss water in, the back of swallowing dissect find in the dissolving of stomach medicine bag shell good; All are normal for blank group, do not occur dead.
Embodiment 2:
Select average weight (150 ± 10) g tilapia for use.Support temporarily in test tank before the test, use the oxygen increasing pump oxygenation, raise domestication with the particulate material that does not contain any medicine, throw something and feed every day once; Through sampling observation, test is with not containing florfenicol in the tilapia tissue.24h stops raising before the administration, feeding method routinely after the administration, and throwing something and feeding every day does not add the blank feedstuff of medicine, and water temperature is controlled at 28 ℃.
The former powder of florfenicol is herded (content 90%) available from Zhe Jiangkang; The florfenicol standard substance are available from China Veterinery Drug Inspection Office (content 99.5%, lot number K030080).
Healthy tilapia is divided into three groups, every group of each five tail, A group: get the former powder of florfenicol and common fish powder feedstuff according to mass ratio 1:36 mixing, adding a small amount of distilled water makes feed granules and (contains the former powder 1.8mg/ of florfenicol grain approximately, the about 65mg/ grain of common fish powder feedstuff), by dosage (12mg/kg) throw something and feed the test fish; B group: with common fish powder feedstuff and the former powder of florfenicol press mass ratio 36:1 mixed even after, encapsulate with gastric solubleness 4# Capsules, become medicament capsule after the encapsulation, each capsule contains common fish powder feedstuff 65mg and the former powder 1.8mg of drug florfenicol approximately, before using the deployed 1.8%(mass percent of medicament capsule leaching) plain agar, make medicament capsule surface coverage one deck agar, after agar surface is sprayed one deck dimethyl-β-DMPT (DMPT), dry standbyly then, obtain the aquatic animal medicine capsule of throwing something and feeding.Producing the pure common fish powder feedstuff capsule that does not contain medicine in addition, to do domestication standby, and its manufacturing step and the above-mentioned aquatic animal medicine capsule of throwing something and feeding is identical, does not just contain the former powder of medicine florfenicol.Before throwing something and feeding, make daily feedstuff feeding with the pure common fish powder feedstuff capsule that does not contain medicine earlier, tamed 3-7 days.Can directly use the aquatic animal medicine capsule of throwing something and feeding to throw something and feed after the domestication, every fish is at every turn by dosage (12mg/kg florfenicol) the capsule test fish of throwing something and feeding.C group is blank, and blank group does not have the medicine of throwing something and feeding, the routine feedstuff of throwing something and feeding.
8h sampling after the administration, every tail fish tail venous blood collection, with the centrifugal 3min of 5000rpm, separated plasma-20 ℃ freezing preservation.
Institute's sample thief is by GB/T20756-2006(HPLC-MS/MS) method carries out sample treatment and detection.Chromatographic column is Waters symmetry C18 chromatographic column (150nm46mm, 5 μ m); Mobile phase is acetonitrile: water=35:65, filters the existing usefulness in degassing back; Flow velocity is 1.0mL/min; Column temperature is 30 ℃; Excitation wavelength is 228nm; Emission wavelength is 280nm; Sample size is 20 μ L.
Prepare the mark-on sample (n=5) of 0.05,0.5,1,2.5,5,10,20 μ g/ml respectively, extraction detects the preparation standard curve.3 times of signal-to-noise ratio computation are detectability, and 10 times of signal to noise ratios are decided to be quantitative limit.
The blood plasma standard curve is as follows: y=1.78 * 10
6X+1.86 * 10
5, R〉and 0.9997, detectability 0.04, quantitative limit 0.12.
Experimental result data uses software 3p97 and WT1.4 to carry out data analysis, as a result shown in the table 1:
Table 1: each organizes the content of the florfenicol in the blood of tilapia
Unit: μ g/ml
Experiment shows, adopt aquatic animal medicine of the present invention to throw something and feed experimental group (B group) that capsule throws something and feeds, and the experimental result data that obtains is more reliable and more stable.Illustrate that this method can be applied to scientific experiments and other related application such as drug metabolism and drug residue, has the reliable advantage of efficient stable.
Claims (3)
1. the aquatic animal medicine preparation method of capsule of throwing something and feeding is characterized in that, may further comprise the steps:
Behind the medicine and fish feed mix homogeneously that needs are thrown something and fed, encapsulate with the gastric solubleness Capsules, become medicament capsule after the encapsulation, again at medicament capsule surface coverage one deck agar, after agar surface is sprayed one deck dimethyl-β-DMPT (DMPT), dry again and obtain the aquatic animal medicine capsule of throwing something and feeding.
2. preparation method according to claim 1 is characterized in that, described gastric solubleness Capsules is gastric solubleness 4# Capsules.
3. an aquatic animal medicine for preparing according to claim 1 or 2 described preparation methoies capsule of throwing something and feeding.
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| CN2013102202249A CN103301085A (en) | 2013-06-04 | 2013-06-04 | Aquatic animal medicine feeding capsule and preparation method thereof |
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| CN2013102202249A CN103301085A (en) | 2013-06-04 | 2013-06-04 | Aquatic animal medicine feeding capsule and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101513227A (en) * | 2009-03-20 | 2009-08-26 | 中国水产科学研究院黑龙江水产研究所 | Composite attraction agent for huchen |
| CN101917867A (en) * | 2007-10-19 | 2010-12-15 | 亚漾妲有限两合公司 | Fish food capsule |
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- 2013-06-04 CN CN2013102202249A patent/CN103301085A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101917867A (en) * | 2007-10-19 | 2010-12-15 | 亚漾妲有限两合公司 | Fish food capsule |
| CN101513227A (en) * | 2009-03-20 | 2009-08-26 | 中国水产科学研究院黑龙江水产研究所 | Composite attraction agent for huchen |
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Application publication date: 20130918 |