CN103293309A - Carbon nano-tube micro-cantilever biosensor for detecting tumor markers - Google Patents

Carbon nano-tube micro-cantilever biosensor for detecting tumor markers Download PDF

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Publication number
CN103293309A
CN103293309A CN2013102621257A CN201310262125A CN103293309A CN 103293309 A CN103293309 A CN 103293309A CN 2013102621257 A CN2013102621257 A CN 2013102621257A CN 201310262125 A CN201310262125 A CN 201310262125A CN 103293309 A CN103293309 A CN 103293309A
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carbon nano
tube
micro
cantilever
base material
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CN2013102621257A
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梁晋涛
黄勇
李桂银
周治德
管明源
马龙飞
黄国银
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Guilin University of Electronic Technology
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Guilin University of Electronic Technology
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Priority to CN2013102621257A priority Critical patent/CN103293309A/en
Publication of CN103293309A publication Critical patent/CN103293309A/en
Priority to CN201410270033.8A priority patent/CN104090104B/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/02—Sensors
    • B81B2201/0214—Biosensors; Chemical sensors

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Abstract

The invention discloses a carbon nano-tube micro-cantilever biosensor which is simple in structure and can be used for detecting various tumor markers. The carbon nano-tube micro-cantilever biosensor comprises a bracket (1), a substrate material (2), a carbon nano-tube (3), a pickup circuit (4) and aptamers (5), wherein the substrate material (2) is fixed on one side of the bracket (1) to form a micro-cantilever structure; the carbon nano-tube (3) grows on the upper surface of the substrate material (2); and the pickup circuit (4) is arranged below the substrate material (2); and the aptamers (5) are attached to the carbon nano-tube (3). According to the carbon nano-tube micro-cantilever biosensor, the tumor markers are detected by using the aptamers modified on the carbon nano-tubes. The micro-cantilever serves as a sensor platform for detecting the tumor markers and easily meets the requirements on high flux, microminiaturization and array formation in detection; and the aim of detecting the tumor markers by combination of various indexes is fulfilled. The micro-cantilever is manufactured by a micro-electromechanical system (MEMS) processing process and can be produced in batches, so that the cost of the biosensor is reduced.

Description

Be used for the carbon nano-tube micro-cantilever biology sensor that tumor markers detects
Technical field
The present invention relates to biomedical engineering field, relate in particular to a kind of carbon nano-tube micro-cantilever biology sensor.
Background technology
Utilize sensor to detect the existing report of method of tumor markers, quality and heat immunosensor, electrochemical immunosensor etc. are arranged.These sensors are general, and false positive rate is higher only at the detection of single tumor markers, and it is not enough to detect degree of accuracy, delays easily or increases the weight of the state of an illness.Develop new detection technique, improve positive rate and accuracy of detection, realize online detection, become the technical matters that needs to be resolved hurrily.
Micro-cantilever beam sensor is fixed together determinand and micro-cantilever by certain mode, the deflection displacement by micro-cantilever or change of resonance frequency realize the signal conversion.Name is called utilizes functionalized carbon nano-tube to be the method for the micro-cantilever beam sensor of sensitive material, and publication number is the application for a patent for invention of CN201010241824.X, and carbon nano-tube is combined with micro-cantilever, realizes the detection to specific gas.This method is highly sensitive, good reproducibility; Weak point is to carry out the functionalization base group modification to carbon nano-tube according to the chemical property of object gas, and operation is comparatively complicated.With the sensor platform of micro-cantilever as the tumor markers detection, utilize the specific reaction between aptamer and the tumor markers to form compound, this compound produces mass effect at carbon nano-tube micro-cantilever biology sensor, and the technology of utilizing this mass effect to realize that tumor markers detects yet there are no report.
Summary of the invention
Technical matters to be solved by this invention provides a kind of carbon nano-tube micro-cantilever biology sensor that detects for tumor markers, and this biology sensor size is little, simple in structure, easy to operate, can realize online many index detections.
In order to solve this technical problem, the present invention realizes detection to tumor markers by modification of nucleic acids aptamers on carbon nano-tube.
Biology sensor of the present invention comprises the aptamer on support, base material, pick-up circuit, carbon nano-tube, the carbon nano-tube.Described base material is fixed on support one side and constitutes micro cantilever structure; Described pick-up circuit part is below base material; Described pick-up circuit part connects into wheatstone bridge form with four voltage dependent resistor (VDR)s; Described carbon nano tube growth is formation carbon nano-tube micro-cantilever beam sensor on micro-cantilever; Described aptamer is to be modified on the carbon nano-tube by hydrophobic effect, also can be to be modified on the carbon nano-tube by π-π superposition; Aptamer and tumor markers form compound by the specific recognition reaction, and the mass effect of utilizing this compound to produce at the micro-cantilever biology sensor realizes detecting.
The present invention utilizes micro-cantilever as the sensor platform that tumor markers detects, and is made into a kind of carbon nano-tube micro-cantilever biology sensor that can realize the kinds of tumors marker detection.
Characteristics of the present invention and advantage:
1, made up a kind of carbon nano-tube micro-cantilever biology sensor that detects tumor markers.This sensor utilizes aptamer and tumor markers reaction formation compound by modification of nucleic acids aptamers on carbon nano-tube, and this compound produces mass effect at micro-cantilever, utilizes this mass effect to realize detection to tumor markers.
2, owing to growing at micro-cantilever carbon nano-tube is arranged, modification of nucleic acids aptamers on the carbon nano-tube, so this biology sensor size is little, simple in structure; The design is new reasonable, and is easy to operate, can realize online detection.
Description of drawings
Fig. 1 is the synoptic diagram that tumor markers detects the carbon nano-tube micro-cantilever biology sensor of usefulness.
Embodiment
Below in conjunction with drawings and Examples the present invention is described in further detail, but is not limited to this embodiment.
Embodiment:
Referring to Fig. 1, Fig. 1 is the synoptic diagram that tumor markers detects carbon nano-tube micro-cantilever biology sensor, comprises support 1, base material 2, carbon nano-tube 3, pick-up circuit 4 and be attached to aptamer 5 above the carbon nano-tube 3.Wherein base material 2 is fixed on support 1 one sides formation micro cantilever structure; Carbon nano-tube 3 be grown in base material 2 above; And pick-up circuit 4 is below base material 2; On carbon nano-tube 3, be modified with one deck aptamer 5 by hydrophobic effect.
The present invention is according to following common process preparation and operation
1, the manufacturing of micro cantilever structure
Micro-cantilever is to be base material 2 with Semiconducting Silicon Materials, is processed into micro cantilever structure.
2, the making of pick-up circuit 4
Pick-up circuit is to utilize microelectronic technique to make the silicon voltage dependent resistor (VDR) at base material 2 lower surfaces, and four voltage dependent resistor (VDR)s are connected into wheatstone bridge form.
3, semi-girder growth and coating carbon nano-tube technology
Upper surface to the base material 2 in the abovementioned steps carries out cleaning treatment, carries out ultrasound wave with acetone, absolute ethyl alcohol, deionized water respectively and cleans, and uses Low Pressure Chemical Vapor Deposition (LPCVD) carbon nano-tube then.Also can use pyrolysismethod, cladding process or additive method carbon nano-tube coating on silica-based.
4, the modification of aptamer on the carbon nano-tube micro-cantilever
Aptamer is modified on the carbon nano-tube by hydrophobic effect, forms a kind of detector probe of energy specific recognition tumor markers, finish carbon nano-tube micro-cantilever biology sensor thereby make up; Aptamer also can be to be modified on the carbon nano-tube by π-π superposition.
The present invention is as follows to the step that tumor markers detects:
(1) on the carbon nano-tube micro-cantilever, makes the detector probe that contains the tumor markers aptamer earlier;
(2) detector probe is put into sample to be tested, tumor markers forms compound by the aptamer on specific reaction and the detector probe and is attached on the micro-cantilever in the sample to be tested.
(3) the tumor-marker substrate concentration is proportionate in the quality of formed compound size and the sample to be tested.
(4) mass change that produces at micro-cantilever of described compound causes micro-cantilever deflection displacement or change of resonance frequency, thereby realizes the detection to tumor markers.

Claims (5)

1. be used for the carbon nano-tube micro-cantilever biology sensor that tumor markers detects, comprise support (1), base material (2), carbon nano-tube (3), pick-up circuit (4); Described base material (2) is fixed on support (1) one side and constitutes micro cantilever structure, carbon nano-tube (3) be grown in base material (2) above, pick-up circuit (4) is below base material (2); It is characterized in that: also comprise the aptamer (5) that is attached to above the carbon nano-tube (3).
2. according to the described biology sensor of claim 1, it is characterized in that: described base material (2) is selected silicon for use.
3. according to the described biology sensor of claim 1, it is characterized in that: described carbon nano-tube (3) be grown in base material (2) above be to adopt earlier the upper surface at base material (2) to carry out cleaning treatment, carry out ultrasound wave with acetone, absolute ethyl alcohol, deionized water respectively and clean, use Low Pressure Chemical Vapor Deposition (LPCVD) carbon nano-tube (3) then.
4. according to the described biology sensor of claim 1, it is characterized in that: the growing method that described carbon nano-tube (3) is grown in base material (2) is pyrolysismethod, perhaps cladding process.
5. according to the described biology sensor of claim 1, it is characterized in that: described pick-up circuit (4) is the piezoresistive effect that utilizes silicon, and four voltage dependent resistor (VDR)s are connected into wheatstone bridge form.
CN2013102621257A 2013-06-27 2013-06-27 Carbon nano-tube micro-cantilever biosensor for detecting tumor markers Pending CN103293309A (en)

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CN201410270033.8A CN104090104B (en) 2013-06-27 2014-06-18 Carbon nano-tube micro-cantilever biosensor for tumor-marker analyte detection that concentration is 0.5-10 mcg/ml

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103543081A (en) * 2013-09-29 2014-01-29 中国科学院半导体研究所 Portable sensing system for early diagnosing liver cancer and functional modification method of portable sensing system
CN104090104A (en) * 2013-06-27 2014-10-08 桂林电子科技大学 Carbon nanotube micro-cantilever biosensor for detecting tumor marker with concentration of 0.5-10[mu]g/mL

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10006888B2 (en) * 2016-04-21 2018-06-26 The Boeing Company MEMS transducers in a phased array coupled to a flexible substrate using carbon nanotubes for conformal ultrasound scanning
CN106092409A (en) * 2016-06-13 2016-11-09 常州大学 Utilize the method that optics quickly measures DNA molecular intermolecular forces
CN108181459A (en) * 2017-11-29 2018-06-19 中国科学技术大学 The micro-cantilever array detection method of microcysin LR based on aptamer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1978315A (en) * 2005-12-09 2007-06-13 清华大学 Method for preparing carbon nano tube array
CN101935008A (en) * 2010-07-30 2011-01-05 中国科学院上海微系统与信息技术研究所 Method of micro cantilever beam sensor using functional carbon nano tubes as sensitive materials
CN203432973U (en) * 2013-06-27 2014-02-12 桂林电子科技大学 Carbon nano-tube micro-cantilever biosensor for detecting tumor markers

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100748408B1 (en) * 2005-06-28 2007-08-10 한국화학연구원 Carbon nanotube biosensors with aptamers as molecular recognition elements and method for sensing target material using the same
KR100830811B1 (en) * 2006-09-18 2008-05-20 충북대학교 산학협력단 Bio-sensor for detecting tumor marker
KR101569891B1 (en) * 2012-02-13 2015-11-27 동국대학교 산학협력단 Sol-gel Chip using Porous Substrate for Entrapping Small Molecules and Screening Method of Small Molecules Specific Material Using thereof
CN103293309A (en) * 2013-06-27 2013-09-11 桂林电子科技大学 Carbon nano-tube micro-cantilever biosensor for detecting tumor markers

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1978315A (en) * 2005-12-09 2007-06-13 清华大学 Method for preparing carbon nano tube array
CN101935008A (en) * 2010-07-30 2011-01-05 中国科学院上海微系统与信息技术研究所 Method of micro cantilever beam sensor using functional carbon nano tubes as sensitive materials
CN203432973U (en) * 2013-06-27 2014-02-12 桂林电子科技大学 Carbon nano-tube micro-cantilever biosensor for detecting tumor markers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李邓化，彭书华，许晓飞: "《智能检测技术及仪表》", 1 March 2007 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104090104A (en) * 2013-06-27 2014-10-08 桂林电子科技大学 Carbon nanotube micro-cantilever biosensor for detecting tumor marker with concentration of 0.5-10[mu]g/mL
CN104090104B (en) * 2013-06-27 2016-08-24 桂林电子科技大学 Carbon nano-tube micro-cantilever biosensor for tumor-marker analyte detection that concentration is 0.5-10 mcg/ml
CN103543081A (en) * 2013-09-29 2014-01-29 中国科学院半导体研究所 Portable sensing system for early diagnosing liver cancer and functional modification method of portable sensing system
CN103543081B (en) * 2013-09-29 2017-04-12 中国科学院半导体研究所 Portable sensing system for early diagnosing liver cancer and functional modification method of portable sensing system

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Application publication date: 20130911