CN103275889A - Ethylparaben-producting fermentation medium of brevibacillus brevis strain and fermentation method - Google Patents
Ethylparaben-producting fermentation medium of brevibacillus brevis strain and fermentation method Download PDFInfo
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Abstract
The invention provides an ethylparaben-producting fermentation medium of a brevibacillus brevis strain, and also discloses a fermentation method which is simple and easy to operate; wherein the brevibacillus brevis strain is brevibacillus brevis strain FJAT-0809-GLX, and the compositions of the fermentation medium are a soybean cake powder 25.18 g/L, DL-malic acid 29.68 g/L, NaCl 13.18 g/L and a peptone 2 g/L, and prepared with ultrapure water in a pH of 7.0. The fermentation condition of brevibacillus brevis strain FJAT-0809-GLX of the invention is optimized, and thus the ethylparaben-producting capability of brevibacillus brevis strain FJAT-0809-GLX is improved, and the fermentation medium and the fermentation method lay a foundation for separation and purification of ethylparaben, mass production, and exploitation of industrial fermentation production, and further can help to avoid pollution to environment when ethylparaben is prepared by a conventional chemical synthesis method.
Description
[technical field]
The present invention relates to the microbial engineering field, relate in particular to fermention medium and fermentation process that a kind of short Bacillus strain produces ethylparoben.
[background technology]
Kind surplus the biologically active substance of the microorganisms of having reported has in the world surpassed ten thousand, wherein have 200 approximately surplus kind in field widespread uses such as medicine, industry, agricultural, herding, breed, food preservation and scientific researches, bringing into play important social benefit.The microorganism biological active substance comprises microbiotic and non-antibiotic biologically active substance, the annual more or less a hundred new microbe active substance of finding, and wherein new non-antibiotic biologically active substance increases year by year, and quantitatively surpasses microbiotic.
Short genus bacillus be a class Gram-positive or variable, thalline is shaft-like, with peritrichous motion, a sporiferous bacterioid.It is reported, the Tyrothricin that short genus bacillus produces generally is made up of the wire linear gramicidins of nine kinds of rrna generations and tyrocidine and the tryptocidines that non-ribosomal produces more than 28 kinds, and these albumen often have different biological activity (Rautenbach etc.); But do not see that short genus bacillus produces the relevant report of ethylparoben at present.Ethylparoben (CAS120-47-8) is for white crystalline powder, odorless or slight special aroma, mildly bitter flavor, bright fiber crops are arranged; Its fungistatic effect to fungi is stronger, but to the fungistatic effect of bacterium a little less than, as antibacterial sanitas, be widely used in the anticorrosion of liquid preparation, semi-solid preparation and foods and cosmetics, also be used for the feed anticorrosion agent.At present, the production of ethylparoben and preparation all adopt the mode of chemosynthesis to carry out suitability for industrialized production mostly, pollute serious.
Therefore, if can adopt short genus bacillus to produce ethylparoben, the pollution that when being expected to avoid the synthetic preparation of existing chemical industry ethylparoben environment is caused.
[summary of the invention]
One of technical problem to be solved by this invention is to provide a kind of short Bacillus strain to produce the fermention medium of ethylparoben.
The present invention is what one of to solve the problems of the technologies described above by the following technical programs:
A kind of short Bacillus strain produces the fermention medium of ethylparoben, the component of this fermention medium: soybean cake powder 25.18g/L, DL-oxysuccinic acid 29.68g/L, NaCl13.18g/L, peptone 2g/L, ultrapure water preparation, pH7.0.
Two of technical problem to be solved by this invention is to provide a kind of short Bacillus strain to produce the fermentation process of ethylparoben.
Two of technical problem to be solved by this invention is to provide a kind of short Bacillus strain to produce the fermentation process of ethylparoben, its operation is as follows: the seed liquor of short Bacillus strain FJAT-0809-GLX is seeded in the 250mL Erlenmeyer flask by 2% inoculum size, and the 100mL fermention medium described in the claim 1 of sterilization is housed in this Erlenmeyer flask; Afterwards described Erlenmeyer flask is placed and carry out fermentation culture on the constant temperature shaking table and get final product 35 ℃ of culture temperature, shaking speed 170r/min, incubation time 24h.
Further, fermention medium is to place 120 ℃ of following sterilization 30min.
Beneficial effect of the present invention is: the fermentation condition to short Bacillus strain FJAT-0809-GLX is optimized, thereby improve the ability that short Bacillus strain FJAT-0809-GLX produces ethylparoben, not only increased the efficient bacteriostatic activity material of new product ethylparoben bacterial classification research data, and laid a good foundation for the exploitation of the separation and purification of ethylparoben, production in enormous quantities and industrial fermentation production; And then can avoid existing chemical industry to synthesize the pollution that when preparing ethylparoben environment is caused.
[description of drawings]
The invention will be further described in conjunction with the embodiments with reference to the accompanying drawings.
Fig. 1 is the figure that influences that different carbon sources are produced ethylparoben among the present invention to bacterial strain FJAT-0809-GLX.
Fig. 2 is the figure that influences that different nitrogen sources is produced ethylparoben among the present invention to bacterial strain FJAT-0809-GLX.
Fig. 3 is the figure that influences that different inorganic salt produce ethylparoben among the present invention to bacterial strain FJAT-0809-GLX.
Fig. 4 is the stereoscopic analysis figure of regression model response surface among the present invention.
[embodiment]
" short Bacillus strain " refers to the applicant and is called in " a kind of new short Bacillus strain and application thereof " disclosed " short Bacillus strain FJAT-0809-GLX " for CN201010296437.6, name in the Chinese patent application number of application on 09 29th, 2010 among the present invention, it was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 08 27th, 2010, and deposit number is CGMCC No.4115.In addition, the article that Che Jianmei etc. the deliver analysis of functional component " the extending longan shelf life functional microorganism FJAT-0809-GLX(Brevibacillus brevis FJAT-0809-GLX) " (Fujian agriculture journal, 2012,27(10): reported 1106-1111) that short Bacillus strain FJAT-0809-GLX has broad-spectrum antibacterial, through separating and identifying that its major function composition is ethylparoben; Thereby illustrated that bacterial strain can produce ethylparoben.And set forth for convenience, below should abbreviate bacterial strain FJAT-0809-GLX as by short Bacillus strain FJAT-0809-GLX.
1 material and method
1.1 material
1.1.1 bacterial strain: short Bacillus strain FJAT-0809-GLX.
1.1.2 main agents and instrument: Agilent automatic sampling highly effective liquid phase chromatographic system; Constant temperature culture vibrator ZHWY-2102C; The ethylparoben standard substance are purchased the sigma company in the U.S., purity 〉=99%; Ultrapure water is produced by Miliipore-system.
1.1.3 substratum:
Seed culture medium: extractum carnis 3.0g/L, peptone 10.0g/L, glucose 10.0g/L, pH7.2-7.4;
Fermention medium: Zulkovsky starch 8.0g/L, soybean cake powder 30.0g/L, peptone 2.0g/L, sucrose 2.0g/L, CaCl25.0g/L, ultrapure water configuration, pH7.1-7.2.
Above substratum all needs at 120 ℃ of autoclaving 30min after preparation is finished.
1.2 method
1.2.1 the preparation of bacterial strain FJAT-0809-GLX fermented liquid ethylparoben solution to be measured
(1) fermented liquid of preparation bacterial strain FJAT-0809-GLX: the single bacterium colony that bacterial strain FJAT-0809-GLX is activated to obtain bacterial strain FJAT-0809-GLX, afterwards with single colony inoculation of this bacterial strain FJAT-0809-GLX in the seed culture medium of 100mL, and be placed in the constant temperature vibration shaking table and cultivate 24h, 30 ℃ of temperature, rotating speed 180rpm/min; The seed liquor of the bacterial strain FJAT-0809-GLX that obtains is seeded in the 250mL Erlenmeyer flask by 2% inoculum size, and the 100mL fermention medium of sterilization is housed in this Erlenmeyer flask; Afterwards this Erlenmeyer flask is placed and carry out fermentation culture on the constant temperature shaking table and get final product culture temperature 30-35 ℃, shaking speed 160-170r/min, incubation time 24h.
(2) preparation of ethylparoben solution to be measured: the fermented liquid 5mL that gets bacterial strain FJAT-0809-GLX places 50mL tool plug centrifuge tube, and adding 20mL alcohol-water-Glacial acetic acid (volume ratio is 70:29.5:0.5) mixed solution, the 15min of water-bath ultrasonic-assisted extraction afterwards, then will extract the extracting solution that obtains and place centrifugal 10min under the 4000r/min, centrifugal end back adopts described mixed solution that extracting solution is settled to 50.0mL, filter, getting the 25mL supernatant liquor is splined on and uses 10mL methyl alcohol in advance, the solid-phase extraction column that 10mL distilled water activation treatment is crossed, keep the nature flow velocity to cross post, with 10mL distilled water flushing pillar, drain, again with 3.0mL methanol-eluted fractions pillar, the control flow rate of liquid is no more than 1mL/min, collect elutriant,, filter as solution to be measured through 0.22 μ m biofilter to 5.0mL with methanol constant volume, measure for high performance liquid chromatograph.
1.2.2 the high performance liquid chromatography of bacterial strain FJAT-0809-GLX fermented liquid ethylparoben detects
Adopt the C18 chromatographic column to measure the ethylparoben solution to be measured of above-mentioned preparation; Moving phase is methanol-water (volume ratio is 65:35), flow velocity 1.0mL/min, and the detection wavelength is 254nm, measuring temperature is 25 ℃, sample size 10 μ L.Retention time per sample and chromatographic peak figure carry out qualitative analysis, carry out quantitative analysis according to its peak area.
1.2.3 short genus bacillus FJAT-0809-GLX Optimizing Conditions of Fermentation
(1) single factor experiment
On the fermention medium basis, the type that changes carbon source, nitrogenous source and inorganic salt is respectively measured the content of ethylparoben in the fermented liquid, and every group arranges 3 repetitions.
(2) optimum fermentation condition is determined in response surface optimization
Plackeet-Burman test: with initial fermentation condition: temperature (A), pH(B), rotating speed (C), air flow (D), DL-oxysuccinic acid (E), peptone (F), Nacl(G), inoculum size (H).As 8 factors of test design, each factor is got 2 levels respectively, each factor value that low-level (1) is initial fermention medium, the content of mensuration ethylparoben.
The steepest hill climbing test: according to the Plackett-Burman testing sieve select to the significant factor of Tyrothricin yield effect, with the path (comprising change direction and change step) that positive negative effect and the effect-size of each remarkable factor are determined the steepest hill climbing test, approach the peak response zone fast.
Center combination design and response surface analysis: with the Plackett-Burman experiment sieving obtain to the significant factor of product content influence as design factor, the factor value that draws with the steepest hill climbing test is as central point, use Minitab15.0 software to carry out test design, and test-results is carried out response surface analysis.Namely adopt polynary quadratic regression equation to come match to express the regression equation of funtcional relationship between factor and the response value, and by the optimum process parameter is sought in the analysis of regression equation, determine the optimum level of key factor.
2 results and analysis
2.1 single factor experiment result
Fixedly nitrogenous source is peptone, respectively 8 kinds of (glucose, Zulkovsky starch, lactose, maltose, glycerine, DL-oxysuccinic acid, sucrose, N.F,USP MANNITOL) different carbon sources is attempted, and calculates ethylparoben content as shown in Figure 1.As shown in Figure 1,8 kinds of different carbon sources are carried out single factor measure ethylparoben content in the fermented liquid, when finding the DL-oxysuccinic acid as carbon source in the fermented liquid ethylparoben content the highest, reach 4.58mg/mL, thereby select for use the DL-oxysuccinic acid as carbon source.
Fixedly carbon source is the DL-oxysuccinic acid, respectively 8 kinds of (yeast extract, peptone, Tryptones, extractum carnis, yeast extract, ammonium oxalate, ammonium sulfate, nutrient broth) different nitrogenous sources is attempted, and calculates ethylparoben content as shown in Figure 2.As shown in Figure 2,8 kinds of different nitrogenous sources are carried out single factor measure ethylparoben content in the fermented liquid, when finding peptone as nitrogenous source in the fermented liquid content of ethylparoben the highest, reach 4.58mg/mL, thereby select for use peptone as nitrogenous source.
Fixedly carbon source is that DL-oxysuccinic acid, nitrogenous source are peptone, respectively to 8 kinds of (Cacl
2, MgSO
4, Nacl, K
2HPO
4, KH
2PO
4, Mncl
2, NaCO
3, FePO
4) different inorganic salt attempt, and calculate ethylparoben content as shown in Figure 3.By shown in Figure 3,8 kinds of different inorganic salt to be carried out single factor measure ethylparoben content in the fermented liquid, ethylparoben content is the highest when finding Nacl as inorganic salt, reaches 3.61mg/mL, therefore selects Nacl to carry out next step research as inorganic salt.
2.2 optimum fermentation condition is determined in response surface optimization
2.2.1 the screening of influence factor
Plackett-Burman testing program with Minitab software design 8 factors 2 levels.According to test design, when fermentation ends, the peak area of high-performance liquid chromatogram determination sample is advanced in preparation ethylparoben solution to be measured and sampling.Calculate ethylparoben content.Plackett-Burman test design scheme and corresponding ethylparoben content see the following form 1, and Plackett-Burman experimental factor level and effect value see Table 2.
Table 1Plackett-Burman test design and result
Annotate: in the table 1, A: temperature (
0C), H B: rotating speed (rmp), C: soybean cake powder (g/L), D: air flow (mL), E:DL-oxysuccinic acid (g/L), F: peptone (g/L), G:Nacl(g/L): inoculum size (%).
As can be seen from Table 1, different factors combine, the ethylparoben content that fermentation produces is different.
Table 2Placekett-Burman experimental factor level and effect value
As shown in Table 2, soybean cake powder, DL-oxysuccinic acid and Nacl as the confidence level of the material impact factor greater than 90%, remarkable to the content influence of ethylparoben in the fermented liquid; Wherein the concentration of DL-oxysuccinic acid, Nacl is positive-effect to the content of ethylparoben, and the influence of soybean cake powder concentration is negative effect.
2.2.2 the establishment of test point, center
By Plackett-Burman test-results design steepest hill climbing test path.According to 3 factorial effect magnitude proportion, set their change direction and step-length design experiment, increase DL-oxysuccinic acid Nacl and the concentration in fermented liquid by certain gradient, reduce the concentration of soybean cake powder in fermented liquid by certain gradient more simultaneously, all the other 5 factors are all got initial value, detect the variation of final fermented liquid ethylparoben content, thereby determine the optimal concentration scope of this 3 factor.Design and the result in steepest hill climbing test path are as shown in table 3.
The design of table 3 steepest hill climbing test and result
As can be seen from Table 3, variation along with soybean cake powder, DL-oxysuccinic acid and Nacl concentration, the first rising of the content of ethylparoben afterwards descends in the fermented liquid, when the concentration of soybean cake powder, DL-oxysuccinic acid and Nacl is respectively 23.5g/L, 28g/L, 11.5g/L, it is 7.16mgm/L that ethylparoben content in the corresponding fermented liquid reaches maximum, therefore point centered by this concentration carries out next step optimization Test.
2.2.3 determining of Optimal compositions of fermentation medium
Be independent variable(s) with soybean cake powder, DL-oxysuccinic acid and Nacl3 important factor, carry out each factor code levels of center combination test design, actual value and result.Center combination test design and the results are shown in Table 4.Regression result is carried out variance analysis (it the results are shown in Table 6), use the t test evaluation for the every influence size to response value of quadratic polynomial, and regression coefficient sees Table 5.As can be seen from Table 5, in each parameter of model, except NaCl once and casein peptone, NaCl and yeast soak between any two mutual of powder, other are every all to have than remarkable influence (confidence level〉90%) product content.Need to prove that the corresponding letters representative is the same in " C, E, the G " in the table 4 and " A, B, C " in the table 5 and the above-mentioned table 2.
Table 4 center combination test design and result
Table 5 regression coefficient table
The variance analysis of table 6 regression model
Y=8.3408+0.09898A-0.01395B+0.1385C-0.07031A*A-0.19583B*B-0.12865C*C+0.05250A*B+0.09250A*C+0.10250B*C。
High model coefficient of determination R
2=0.9659, show that this model can explain the variation of the content of 96.59% product, the regression fit degree is better, can predict the content of ethylparoben in the fermented liquid with this model.Utilize minitab software that regression model is carried out response surface analysis, obtain the stereoscopic analysis figure of a response surface, as shown in Figure 4.
By the map analysis of Fig. 4 response surface as can be known, response surface presents convex-shaped, proves to have best culture condition.By to mountain range ridge analysis when the mass concentration of soybean cake powder, DL-oxysuccinic acid and Nacl is respectively 25.18g/L, 29.68g/L, 13.18g/L, the response face amount reach maximum value, namely the ethylparoben mass concentration is 8.34mg/mL in the fermented liquid.
Microbial fermentation is a complex physical biological process, when thalline is in different nutritional conditions, can directly influences the physiological metabolism of thalline, thereby influence the output of active substance.Improve the output of ethylparoben in the bacterial strain FJAT-0809-GLX fermented liquid, fermentation condition is optimized is absolutely necessary.The present invention optimizes the fermentation condition of bacterial strain FJAT-0809-GLX, determined that by single factor and response surface method its best fermentation product ethylparoben substratum is: soybean cake powder 25.18g/L, DL-oxysuccinic acid 29.68g/L, NaCl13.18g/L, peptone 2g/L, the ultrapure water preparation, pH7.0; Culture condition is: 35 ℃ of culture temperature, shaking speed 170r/min, incubation time 24h.The present invention has increased the efficient bacteriostatic activity material of new product ethylparoben bacterial classification research data, for the exploitation of separation and purification, production in enormous quantities and the industrial fermentation production of ethylparoben is laid a good foundation; Thereby the pollution that in the time of can avoiding the synthetic preparation of existing chemical industry ethylparoben environment is caused.
Claims (3)
1. the fermention medium of one kind short Bacillus strain product ethylparoben is characterized in that: the component of this fermention medium: soybean cake powder 25.18g/L, DL-oxysuccinic acid 29.68g/L, NaCl13.18g/L, peptone 2g/L, ultrapure water preparation, pH7.0.
2. one kind short Bacillus strain produces the fermentation process of ethylparoben, it is characterized in that: the seed liquor of short Bacillus strain FJAT-0809-GLX is seeded in the 250mL Erlenmeyer flask by 2% inoculum size, and the 100mL fermention medium described in the claim 1 of sterilization is housed in this Erlenmeyer flask; Afterwards described Erlenmeyer flask is placed and carry out fermentation culture on the constant temperature shaking table and get final product 35 ℃ of culture temperature, shaking speed 170r/min, incubation time 24h.
3. short Bacillus strain according to claim 2 produces the fermentation process of ethylparoben, and it is characterized in that: described fermention medium is to place 120 ℃ of following sterilization 30min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104732084A (en) * | 2015-03-20 | 2015-06-24 | 上海交通大学 | Model construction method for reducing rapidly digestible starch content on basis of response surface method |
| CN106720391A (en) * | 2016-12-01 | 2017-05-31 | 统企业(中国)投资有限公司昆山研究开发中心 | Industrialization aerated frozen mousse body and its production technology |
| CN107955829A (en) * | 2017-11-07 | 2018-04-24 | 中南民族大学 | A kind of method for promoting bacillus production spore using response phase method rapid Optimum metal ion |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955902A (en) * | 2010-09-29 | 2011-01-26 | 福建省农业科学院农业生物资源研究所 | New brevibacillus brevis strain and application thereof |
| CN102559786A (en) * | 2012-01-20 | 2012-07-11 | 福建省农业科学院农业生物资源研究所 | Method for preparing ethylparaben from Brevibacillus brevis strain |
-
2013
- 2013-04-27 CN CN2013101545631A patent/CN103275889A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955902A (en) * | 2010-09-29 | 2011-01-26 | 福建省农业科学院农业生物资源研究所 | New brevibacillus brevis strain and application thereof |
| CN102559786A (en) * | 2012-01-20 | 2012-07-11 | 福建省农业科学院农业生物资源研究所 | Method for preparing ethylparaben from Brevibacillus brevis strain |
Non-Patent Citations (3)
| Title |
|---|
| 车建美等: "保鲜功能微生物FJAT-0809-GLX对龙眼果实保鲜方法的优化", 《中国农学通报》, no. 23, 31 December 2011 (2011-12-31) * |
| 陈冰冰: "短短芽孢杆菌FJAT-0809-GLX功能成分羟苯乙酯含量测定及其生产发酵条天优化", 《福建农林大学硕士学位论文》, 21 March 2013 (2013-03-21) * |
| 陈凯 等: "短短芽孢杆菌(brevibacillus brevis)XDH菌株发酵培养基配方的研究", 《山东农业大学学报 自然科学版》, 31 December 2006 (2006-12-31), pages 190 - 195 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104732084A (en) * | 2015-03-20 | 2015-06-24 | 上海交通大学 | Model construction method for reducing rapidly digestible starch content on basis of response surface method |
| CN104732084B (en) * | 2015-03-20 | 2017-07-11 | 上海交通大学 | The model building method of rapid digestion content of starch is reduced based on response phase method |
| CN106720391A (en) * | 2016-12-01 | 2017-05-31 | 统企业(中国)投资有限公司昆山研究开发中心 | Industrialization aerated frozen mousse body and its production technology |
| CN106720391B (en) * | 2016-12-01 | 2020-06-26 | 统一企业(中国)投资有限公司昆山研究开发中心 | Industrialized aerated frozen mousse body and production process thereof |
| CN107955829A (en) * | 2017-11-07 | 2018-04-24 | 中南民族大学 | A kind of method for promoting bacillus production spore using response phase method rapid Optimum metal ion |
| CN107955829B (en) * | 2017-11-07 | 2021-02-02 | 中南民族大学 | Method for promoting spore production of bacillus by quickly optimizing metal ions by response surface method |
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Application publication date: 20130904 |