CN103275181A - Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof - Google Patents
Tuna ground meat polypeptide angiogenesis inhibiting factor as well as preparation method and application thereof Download PDFInfo
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- CN103275181A CN103275181A CN2013101946054A CN201310194605A CN103275181A CN 103275181 A CN103275181 A CN 103275181A CN 2013101946054 A CN2013101946054 A CN 2013101946054A CN 201310194605 A CN201310194605 A CN 201310194605A CN 103275181 A CN103275181 A CN 103275181A
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Abstract
The invention discloses a tuna ground meat polypeptide angiogenesis inhibiting factor as well as a preparation method and an application thereof. The amino acid sequence of the polypeptide angiogenesis inhibiting factor is Trp-Val-Thr-Gly-Ile (WVTGI), and ESI/MS (electrospray ionization/mass spectrometry) detection shows that a molecular ion peak (m/z) is 575.42Da ([M+H]+). The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for effectively inhibiting angiogenesis of chorioallantoic membrane (CAM) of chicken embryo and multiplication of prostate cancer cell DU-145. The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for preparing a medicine used for inhibiting angiogenesis and preventing and treating prostate cancer.
Description
Technical field
The present invention relates to a kind of tuna meat mincing polypeptide class Angiostatin, the invention still further relates to the preparation method of this tuna meat mincing polypeptide class Angiostatin, the invention still further relates to the purposes of this tuna meat mincing polypeptide class Angiostatin.
Background technology
Vasculogenesis plays a significant role in normal physiological processes such as human development, tissue repair, also is one of multiple important pathological characters such as tumour.The professor Folkman of U.S. Harvard Medical School thinks that growth of tumor depends on the generation of new vessel, can reach the purpose for the treatment of tumour by the vasculogenesis that suppresses tumour.At present, this viewpoint confirmed by a large amount of experiments, and (tumor angiogenesis inhibitor TAI) also becomes the focus of research and be the medicine of target treatment tumour----angiogenesis inhibitor with the blood vessel.
Tuna is one of important operation fingerling of world's sea going fisheries, and annual production surpasses 6,000,000 tons, accounts for more than 70% of high sea fishery ultimate production.Tankage such as the dark-coloured meat that produces in the tuna course of processing, internal organ, fish head and fish-skin account for gross weight 50%～70%.Discover that crude protein content is the excellent protein source than higher in the tuna tankage, utilize the protein in the tuna tankage can produce multiple polypeptide with physiologically active, as blood pressure lowering peptide, anti-oxidation peptide, immune peptide etc.
But, the applicant discovers, be raw material with tuna tankage meat mincing, the technical study of utilizing zymolysis technique to prepare polypeptide class Angiostatin is in the blank stage, and is material preparation high reactivity polypeptide class Angiostatin and uses and do not appear in the newspapers especially with tuna tankage meat mincing zymolyte.
Summary of the invention
First technical problem to be solved by this invention is that the state of the art at the utilization of above-mentioned tuna meat mincing provides a kind of tuna meat mincing polypeptide class Angiostatin, this Angiostatin can effectively suppress chick chorioallantoic membrane (CAM) vasculogenesis, and the propagation of prostate cancer cell DU-145 is also had good inhibition effect.
Second technical problem to be solved by this invention provides a kind of preparation method of tuna meat mincing polypeptide class Angiostatin.
The 3rd technical problem to be solved by this invention provides a kind of tuna meat mincing polypeptide class Angiostatin and suppresses vasculogenesis in preparation, prevents and treats the application in the medicine of prostate cancer.
The technical scheme that the present invention takes for above-mentioned first technical problem of solution is: a kind of tuna meat mincing polypeptide class Angiostatin, the aminoacid sequence that it is characterized in that this Angiostatin is Trp-Val-Thr-Gly-Ile(WVTGI), ESI/MS detects and provides molecular ion peak
M/z575.42 Da([M+H]
+).
The technical scheme that the present invention takes for above-mentioned second technical problem of solution is: a kind of preparation method of tuna meat mincing polypeptide class Angiostatin is characterized in that may further comprise the steps:
1) gets the tuna meat mincing, press solid-liquid ratio 1:3 ~ 1:5 and add damping fluid, regulate pH value to 7.5 ~ 8.0, in 35 ~ 40 ℃ of insulation 5-10 min, homogenate;
2) according to tuna meat mincing quality, add trypsin trypsin), enzyme concentration is 2000 ~ 2500 u/g, hydrolysis temperature is 35 ~ 40 ℃, enzymolysis time 4 ~ 6 h; Enzymolysis solution is warming up to 90 ~ 95 ℃, and after this temperature keeps 10 ~ 15min, makes enzyme-deactivating; Centrifugal 15 ~ 20 min of deactivation enzymolysis solution 9000 ~ 10000 r/min get supernatant liquor, and freeze-drying gets enzymolysis product.
3) with the preparation tuna meat mincing enzymolysis product successively through ultrafiltration and chromatography, obtain tuna meat mincing polypeptide class Angiostatin.
As preferably, the tuna in the described step 1) be stripped tuna (
Katsuwonus pelamis).
As preferably, the damping fluid in the described step 1) is that pH is 8.0 K
2HPO
4-KH
2PO
4Damping fluid.
As preferably, described step 2) enzyme activity 〉=1.0 * 10 trypsin trypsin in)
4U/g.
As improvement, the ultrafiltration of described step 3) and the detailed process of chromatography are:
Ultrafiltration: tuna meat mincing enzymolysis product is dissolved in distilled water, be made into the solution that concentration is 15 ~ 20 mg/mL, under the working temperature of the operating pressure of 0.1 ~ 0.15 MPa and 20 ~ 25 ℃, adopt 1 kDa ultra-filtration membrane to carry out uf processing, collect molecular weight less than 1 kDa part, freeze-drying is the ultrafiltration zymolyte;
Chromatography: above-mentioned ultrafiltration zymolyte is dissolved in distilled water is made into the solution that concentration is 10 ~ 15 mg/mL, separate through anionite-exchange resin, the NaCl solution of water, 0.09 ~ 0.11 mol/L, 0.45 ~ 0.55 mol/L and 0.90 ~ 1.10 mol/L carries out gradient elution, collect elution fraction according to the absorbancy curve under 220 nm, wherein, be the ion exchange chromatography zymolyte to the highest component of chick chorioallantoic membrane (CAM) angiogenesis suppression action; Above-mentioned ion exchange chromatography zymolyte is made into the solution of 8 ~ 12 mg/mL with distilled water, separate through gel filtration chromatography, carry out wash-out with distilled water, collect elution fraction according to the absorbancy curve under 220 nm, wherein, be the gel chromatography zymolyte to the highest component of CAM angiogenesis suppression action, above-mentioned gel chromatography zymolyte is made into the solution of 45 ~ 55 μ g/mL with distilled water, utilize RPLC (RP-HPLC) to carry out purifying, get 1 high angiogenesis suppression action peptide T rp-Val-Thr-Gly-Ile(WVTGI according to the restraining effect to the CAM vasculogenesis), ESI/MS detects and provides molecular ion peak
M/z575.42 Da([M+H]
+).
Preferably, described anionite-exchange resin is the DEAE-52 Mierocrystalline cellulose, and described gel is sephadex G-25; Described RPLC condition is: sample size 10 ~ 15 μ L; Chromatographic column is Zorbax C18(250 mm * 4.6 mm, 5 μ m); Moving phase is 30 % acetonitriles; The ultraviolet detection wavelength is 220 nm.
The present invention for above-mentioned the 3rd technical scheme that technical problem is taked of solution is: a kind of application of tuna meat mincing polypeptide class Angiostatin, it is characterized in that Trp-Val-Thr-Gly-Ile(WVTGI) 1 μ g/ only, 10 μ g/ are only and under the 100 μ g/ concentration only, and the inhibiting rate of CAM vasculogenesis is respectively 42.7%, 64.0% and 87.6%; IC to prostate cancer cell DU-145
50Be 0.22 mg/mL; Trp-Val-Thr-Gly-Ile(WVTGI) have advantages such as safe without toxic side effect and activity are strong, can be used for preparing the inhibition vasculogenesis, prevent and treat the medicine of prostate cancer.
Compared with prior art, the invention has the advantages that: craft science of the present invention is reasonable, select trypsin trypsin for use) as the enzymolysis enzyme, by biologic enzymolysis method nexus ultrafiltration classification simultaneously, macroporous resin desalination and chromatographic refining, enzymolysis process is easily monitored, and the polypeptide class Angiostatin that makes simultaneously has higher activity; Compare with the polypeptide class Angiostatin of chemosynthesis, the polypeptide class Angiostatin that the present invention makes has safe without toxic side effect and advantages such as activity is strong, can be used for preparing the inhibition vasculogenesis, prevents and treats the medicine of prostate cancer.
Description of drawings
Fig. 1 is the anionite-exchange resin DEAE-52 Mierocrystalline cellulose chromatography figure of ultrafiltration zymolyte of the present invention;
Fig. 2 is sephadex G-25 tomographic map of ion exchange chromatography zymolyte of the present invention;
Fig. 3 is RPLC (RP-HPLC) analysis chart of gel chromatography zymolyte of the present invention;
Fig. 4 is Trp-Val-Gly-Thr-Ile(WVGTI of the present invention) RPLC (RP-HPLC) figure;
Fig. 5 is Trp-Val-Gly-Thr-Ile(WVGTI of the present invention) mass spectrum (ESI/MS).
Embodiment
Describe in further detail below in conjunction with the present invention of embodiment.
A kind of preparation method of tuna meat mincing polypeptide class Angiostatin, preparation technology's flow process is as follows: the preparation of tuna meat mincing " enzymolysis " zymolytes " membrane ultrafiltration " ion exchange chromatographies " gel permeation chromatography " high performance liquid chromatography, analysis " tuna meat mincing polypeptide class Angiostatin.
Embodiment 1:
1) gets the tuna meat mincing, press solid-liquid ratio 1:3 ~ 1:5 and add K
2HPO
4-KH
2PO
4Damping fluid (pH 8.0) is in 38 ℃ of insulation 10 min, homogenate;
2) according to tuna meat mincing quality, add trypsin trypsin), enzyme concentration is 2500 u/g, hydrolysis temperature is 38 ℃, enzymolysis time 5 h; Enzymolysis solution is warming up to 90 ℃, and after this temperature keeps 10 min, makes enzyme-deactivating; The deactivation enzymolysis solution is got supernatant liquor in centrifugal 20 min of 10000 r/min, and freeze-drying gets enzymolysis product.
3) with step 2) enzymolysis product of gained is successively through ultrafiltration and chromatography, obtains tuna meat mincing polypeptide class Angiostatin, utilizes its structure of amino acid sequence analysis and mass spectroscopy, and detailed process is:
1. ultrafiltration: adopt 1 kDa ultra-filtration membrane to carry out uf processing under the working temperature of the operating pressure of 0.1 ~ 0.15 MPa and 25 ℃ tuna meat mincing protein enzymatic hydrolyzate, collect molecular weight less than 1 kDa part, freeze-drying is the ultrafiltration zymolyte;
2. anion-exchange chromatography: above-mentioned ultrafiltration zymolyte is dissolved in distilled water is made into the solution that concentration is 15 mg/mL, separate through DEAE-52 Mierocrystalline cellulose anionite-exchange resin, the NaCl solution of water, 0.1 mol/L, 0.5 mol/L and 1.0 mol/L carries out wash-out, collect elution fraction according to the absorbancy curve under 220 nm, wherein, to the highest component of chick chorioallantoic membrane (CAM) angiogenesis suppression action be ion exchange chromatography zymolyte (F5) (Fig. 1);
3. gel chromatography chromatography: the solution that above-mentioned ion exchange chromatography zymolyte (F5) is made into 10 mg/mL with distilled water, through sephadex G-25 column chromatography for separation, carry out wash-out with distilled water, collect elution fraction according to the absorbancy curve under 220 nm, wherein, to the highest component of chick chorioallantoic membrane (CAM) angiogenesis suppression action be gel chromatography zymolyte (F52) (Fig. 2).
4. high performance liquid chromatography is refining: the gel chromatography zymolyte (F52) of above-mentioned preparation is made into the solution of 50 μ g/mL with distilled water, utilizes RPLC (RP-HPLC) to carry out purifying (condition: sample size 15 μ g; Chromatographic column is Zorbax C18(250 mm * 4.6 mm, 5 μ m); Moving phase: 30 % acetonitriles; Ultraviolet detection wavelength 220 nm), get 1 high angiogenesis suppression action polypeptide (F52-II) according to the restraining effect to chick chorioallantoic membrane (CAM) vasculogenesis.(see figure 3).
5. it is the simple spike (see figure 4) that structure determination: RP-HPLC detects the high angiogenesis suppression action polypeptide of collecting, utilize albumen/peptide sequence analysis-e/or determining aminoacid sequence to be Trp-Val-Thr-Gly-Ile(WVTGI), ESI/MS detects and provides molecular ion peak
M/z575.42 Da([M+H]
+).
With the above-mentioned tuna meat mincing albumen tumor protein p53 Trp-Val-Thr-Gly-Ile(WVTGI that makes) carry out the proliferation inhibition test of prostate cancer cell DU-145.Experimental result shows: this polypeptide is to the IC of prostate cancer cell DU-145
50Be 0.22 mg/mL.
With the peptide T rp-Val-Thr-Gly-Ile(WVTGI that makes) do to suppress the mensuration of chick chorioallantoic membrane (CAM) vasculogenesis, measuring method [is recorded in " Zhongshan University's journal (natural science edition) " with reference to " improved chick chorioallantoic membrane technique-no air chamber are hatched method " such as He Guoan, Luo Jinxian, Zhang Tianyuan, 2003 the 2nd (the 42nd volume): 126-128 page or leaf], the filter paper size is 3 mm * 3 mm during mensuration; The application of sample preincubation time is 4 days; The application of sample amount: Trp-Val-Thr-Gly-Ile(WVTGI) be respectively 1 μ g/ only, 10 μ g/ only, 100 μ g/ only; 0.01mol/L the negative contrast of the phosphate buffered saline buffer of pH 7.6 (PBS); The 5 μ g/ positive contrast of chondroitin sulfate (CS) only.Continue hatching 24h behind the application of sample, giving centered by the snack made with traditional Chinese medicines, with radius at interval 5 mm CAM is divided into 3 zones, count each regional blood vessel number, be calculated as follows the vasculogenesis inhibiting rate:
Vasculogenesis inhibiting rate=(negative control group vessel branch count an administration group vessel branch count)/negative control group vessel branch counts * and 100%
The result shows, Trp-Val-Thr-Gly-Ile(WVTGI) can significantly suppress the vasculogenesis of CAM and be dose-dependence, 1 μ g/ only, 10 μ g/ are only and under the 100 μ g/ concentration only, to the inhibiting rate of CAM vasculogenesis be respectively 42.7%, 64.0% and 87.6%(table 1).
Table 1 tuna meat mincing polypeptide class Angiostatin Trp-Val-Thr-Gly-Ile(WVTGI) to the restraining effect of CAM vasculogenesis
| Group | Chicken embryo number | Dosage (μ g/ only) | Vessel branch count (
 |
Inhibiting rate (%) |
| Contrast | 5 | -- | 72.3±5.2 | -- |
| Chondroitin sulfate | 5 | 5 | 29.3±1.9 | 59.5 |
| WVTGI | 5 | 1 | 41.4±1.9 | 42.7 |
| WVTGI | 5 | 10 | 26.0±2.8 | 64.0 |
| WVTGI | 5 | 100 | 8.97±0.5 | 87.6 |
At last, be noted that still that what more than enumerate only is a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
＜110〉Oceanography Institute Of Zhejiang
＜120〉a kind of tuna meat mincing polypeptide class Angiostatin and its production and use
<130> zjou-sy-005
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 5
<212> PRT
＜213〉artificial sequence
<400> 1
Trp Val Thr Gly Ile
1 5
Claims (9)
1. tuna meat mincing polypeptide class Angiostatin, the aminoacid sequence that it is characterized in that this tuna meat mincing polypeptide class Angiostatin is Trp-Val-Thr-Gly-Ile SEQ ID NO:1, ESI/MS detects and provides molecular ion peak
M/z575.42 Da([M+H]
+).
2. the preparation method of the described a kind of tuna meat mincing polypeptide class Angiostatin of claim 1 is characterized in that may further comprise the steps:
1) gets the tuna meat mincing, press solid-liquid ratio 1:3 ~ 1:5 and add damping fluid, regulate pH value to 7.5 ~ 8.0, in 35 ~ 40 ℃ of insulation 5 ~ 10 min, homogenate;
2) according to tuna meat mincing quality, add trypsin trypsin), enzyme concentration is 2000 ~ 2500 u/g, hydrolysis temperature is 35 ~ 40 ℃, enzymolysis time 4 ~ 6 h; Enzymolysis solution is warming up to 90 ~ 95 ℃, and after this temperature keeps 10 ~ 15min, makes enzyme-deactivating; Centrifugal 15 ~ 20 min of enzymolysis solution 9000 ~ 10000 r/min get supernatant liquor, and freeze-drying gets enzymolysis product;
3) with the preparation tuna meat mincing enzymolysis product successively through ultrafiltration and chromatography, obtain tuna meat mincing polypeptide class Angiostatin.
3. preparation method according to claim 2, it is characterized in that in the described step 1) in tuna be stripped tuna (
Katsuwonus pelamis).
4. preparation method according to claim 2 is characterized in that the damping fluid in the described step 1) is that pH is 8.0 K
2HPO
4-KH
2PO
4Damping fluid.
5. preparation method according to claim 2 is characterized in that tryptic enzyme activity 〉=1.0 * 10 in the described step 3)
4U/g.
6. preparation method according to claim 2 is characterized in that the ultrafiltration of described step 3) and the detailed process of chromatography are:
Ultrafiltration: tuna meat mincing enzymolysis product is dissolved in distilled water, be made into the solution that concentration is 15 ~ 20 mg/mL, under the working temperature of the operating pressure of 0.1 ~ 0.15 MPa and 20 ~ 25 ℃, adopt 1 kDa ultra-filtration membrane to carry out uf processing, collect molecular weight less than 1 kDa part, freeze-drying gets the ultrafiltration zymolyte;
Chromatography: above-mentioned ultrafiltration zymolyte is dissolved in distilled water is made into the solution that concentration is 10 ~ 15 mg/mL, separate through anionite-exchange resin, water, 0.09 ~ 0.11 mol/L, 0.45 ~ 0.55 mol/L and 0.90 ~ 1.10 mol/L NaCl solution carry out gradient elution, collect elution fraction according to the absorbancy curve under 220 nm, wherein, be the ion exchange chromatography zymolyte to the highest component of chick chorioallantoic membrane (CAM) angiogenesis suppression action; Above-mentioned ion exchange chromatography zymolyte is made into the solution of 8 ~ 12 mg/mL with distilled water, separate through gel filtration chromatography, carry out wash-out with distilled water, collect elution fraction according to the absorbancy curve under 220 nm, wherein, be the gel chromatography zymolyte to the highest component of CAM angiogenesis suppression action, above-mentioned gel chromatography zymolyte is made into the solution of 45 ~ 55 μ g/mL with distilled water, utilize RPLC (RP-HPLC) to carry out purifying, get 1 high angiogenesis suppression action peptide T rp-Val-Thr-Gly-Ile SEQ ID NO:1 according to the restraining effect to the CAM vasculogenesis, ESI/MS detects and provides molecular ion peak
M/z575.42 Da([M+H]
+).
7. preparation method according to claim 6 is characterized in that described anionite-exchange resin is the DEAE-52 Mierocrystalline cellulose, and described gel is sephadex G-25; Described RPLC condition is: sample size 10 ~ 15 μ L; Chromatographic column is Zorbax C18(250 mm * 4.6 mm, 5 μ m); Moving phase is 30 % acetonitriles; The ultraviolet detection wavelength is 220 nm.
8. the preparation method of a kind of tuna meat mincing polypeptide class Angiostatin according to claim 2, it is characterized in that prepared polypeptide class Angiostatin Trp-Val-Thr-Gly-Ile SEQ ID NO:1 1 μ g/ only, 10 μ g/ are only and under the 100 μ g/ concentration only, and the inhibiting rate of CAM vasculogenesis is respectively 42.7%, 64.0% and 87.6%; IC to prostate cancer cell DU-145
50Be 0.22 mg/mL.
9. the described a kind of tuna meat mincing polypeptide class Angiostatin of claim 1 is for the preparation of the purposes that suppresses vasculogenesis, prevents and treats the prostate cancer medicine.
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| CN104774899A (en) * | 2015-04-24 | 2015-07-15 | 浙江海洋学院 | Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein |
| CN104774898A (en) * | 2015-04-24 | 2015-07-15 | 浙江海洋学院 | Application of anti-cervical-carcinoma polypeptide prepared by using tuna dark-meat proteins |
| CN105624247A (en) * | 2016-02-29 | 2016-06-01 | 浙江海洋学院 | Preparation method for activator of Nrf2-ARE pathway in tuna high F ratio oligopeptide |
| CN105669853A (en) * | 2016-02-29 | 2016-06-15 | 浙江海洋学院 | Activator for Nrf2-ARE pathway in high fischer ratio oligo-peptide from tuna |
| CN106544388A (en) * | 2016-11-08 | 2017-03-29 | 广东海洋大学 | A kind of Skipjack antioxidant composition and preparation method thereof |
| CN110732018A (en) * | 2019-10-14 | 2020-01-31 | 浙江海洋大学 | preparation method of tuna meat ACE inhibitory peptide chewable tablets |
| CN113801194B (en) * | 2021-10-15 | 2023-06-20 | 浙江海洋大学 | Hypolipidemic peptide of tuna roe and application thereof |
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| CN104774899A (en) * | 2015-04-24 | 2015-07-15 | 浙江海洋学院 | Method for preparing cervical cancer resistant polypeptide from tuna dark meat protein |
| CN104774898A (en) * | 2015-04-24 | 2015-07-15 | 浙江海洋学院 | Application of anti-cervical-carcinoma polypeptide prepared by using tuna dark-meat proteins |
| CN104774898B (en) * | 2015-04-24 | 2020-07-14 | 浙江海洋学院 | Application of tuna dark meat protein anti-cervical cancer polypeptide |
| CN105624247A (en) * | 2016-02-29 | 2016-06-01 | 浙江海洋学院 | Preparation method for activator of Nrf2-ARE pathway in tuna high F ratio oligopeptide |
| CN105669853A (en) * | 2016-02-29 | 2016-06-15 | 浙江海洋学院 | Activator for Nrf2-ARE pathway in high fischer ratio oligo-peptide from tuna |
| CN105624247B (en) * | 2016-02-29 | 2020-08-25 | 浙江海洋学院 | Preparation method of Nrf2-ARE pathway activator in tuna high-F-value oligopeptide |
| CN105669853B (en) * | 2016-02-29 | 2020-08-25 | 浙江海洋学院 | Nrf2-ARE pathway activator in tuna high F value oligopeptide |
| CN106544388A (en) * | 2016-11-08 | 2017-03-29 | 广东海洋大学 | A kind of Skipjack antioxidant composition and preparation method thereof |
| CN106544388B (en) * | 2016-11-08 | 2020-07-03 | 广东海洋大学 | Skipjack antioxidant compound and preparation method thereof |
| CN110732018A (en) * | 2019-10-14 | 2020-01-31 | 浙江海洋大学 | preparation method of tuna meat ACE inhibitory peptide chewable tablets |
| CN110732018B (en) * | 2019-10-14 | 2023-04-07 | 浙江海洋大学 | Preparation method of tuna meat ACE inhibitory peptide chewable tablets |
| CN113801194B (en) * | 2021-10-15 | 2023-06-20 | 浙江海洋大学 | Hypolipidemic peptide of tuna roe and application thereof |
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