CN103275138B - 16 carbon diacetyls are without double bond lactone type sophorolipid and application thereof - Google Patents
16 carbon diacetyls are without double bond lactone type sophorolipid and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of compound 16 carbon diacetyl without double bond lactone type sophorolipid and preparing the application in prevention and therapy liver cancer, lung cancer, the esophageal carcinoma, cancer of the stomach and breast cancer medicines.Experiment proves, compound of the present invention is when concentration is 50mg/kg body weight, stronger restraining effect is had to human hepatoma HepG2 cell, people's lung cancer cell A-549, people cancer of the stomach SGC7901 cell, human esophagus cancer KYSE109 cell and mammary cancer MDA-MB-435 cell, for preparing anti-kinds of tumors and toxic side effect is less, the more obvious antitumour drug of effect provides the foundation and approach, be extremely important.
Description
Technical field
The present invention relates to sophorolipid compound and application thereof, particularly relate to 16 carbon diacetyls and (be called for short: C16:0DLSL) and preparing in cancer therapy drug apply without double bond lactone type sophorolipid.
Background technology
Sophorolipid is a kind of important bio-surfactant, and the research for it starts from twentieth century five, the sixties, is mainly obtained by fermentable.
Sophorolipid mainly contains two kinds of basic structures, as shown below:
Structural formula (the R=Actyl of sophorolipid; Zuo Tu: acid type sophorolipid; Right figure: lactone type sophorolipid)
Sophorolipid is produced by the yeast of several non-virulent, for by the molecular mixture of the sophorolipid of a series of similar.The sophorolipid molecule of different sorts and structure has different physics and chemistry activity and biological activity.Experiment confirms, lactone type sophorolipid has the ability and anti-microbial activity that better reduce surface tension of liquid, and acid type sophorolipid has better water-soluble and foaming power.
In recent years, abroad for the research of sophorolipid as medicine, particularly there are some bibliographical informations antitumor drug aspect.People's reports such as Scholz and Mehta; crude product sophorolipid and sophorolipid derivative can suppress the propagation of human leukemic HL60 and people's head & neck cancer cell; and the anti-tumor activity demonstrating sophorolipid is relevant with the ethanoyl of sophorolipid; the ethanoyl of sophorolipid is removed by chemical reaction; find that its anti-tumor activity obviously reduces, but its antitumor mechanism is not further studied.People's reports such as Isoda, sophorolipid can cause the cytodifferentiation of HL60 Leukemia Cell Lines and the activity of arrestin kinase c.In addition, sophorolipid can be used as the immunomodulator of Parkinson's disease, senile dementia, psoriasis, treating AIDS, also can be used as antiviral immunostimulation.
But; through document and patent retrieval; sophorolipid sterling molecule is if 16 carbon diacetyls are without double bond lactone type sophorolipid; or with the pharmaceutical composition that this compound is activeconstituents, or utilize this compound in preparation treatment and prevent liver cancer, application in the medicine of lung cancer, the esophageal carcinoma, cancer of the stomach, cervical cancer and mammary cancer has no relevant report.
Summary of the invention
16 carbon diacetyls are the object of the present invention is to provide (to be called for short: C16:0DLSL) and preparing in cancer therapy drug apply without double bond lactone type sophorolipid.
16 carbon diacetyls of the present invention are without double bond lactone type sophorolipid, and its structure is as shown in formula I:
Above-mentioned 16 carbon diacetyls are utilize Wickerhamiella domercqiae var. sophorolipid (Wickerhamielladomercqiae var.sophorolipid) CGMCC No.1576 after liquid fermenting without double bond lactone type sophorolipid, and from liquid fermentation production, separation and purification obtains; Wherein: described Wickerhamiella domercqiae var. sophorolipid (Wickerhamiella domercqiae var.sophorolipid) CGMCC No.1576 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (Institute of Microorganism, Academia Sinica on December 26th, 2005, BeiJing, China), related strain patent is shown in " a kind of Wickerhamiella domercqiae var. sophorolipid and application (patent No. 200610042190.9) thereof of producing sophorolipid ".
16 carbon diacetyls of the present invention are preparing the application in prevention and therapy liver cancer, lung cancer, the esophageal carcinoma, cancer of the stomach and breast cancer medicines without double bond lactone type sophorolipid.
Contriver finds that 16 carbon diacetyls of the present invention have preventive and therapeutic action without double bond lactone type sophorolipid to liver cancer, lung cancer, cancer of the stomach, the esophageal carcinoma, mammary cancer.Experiment proves, compound of the present invention is when concentration is 50mg/kg body weight, stronger restraining effect (35 ~ 40%) is had to human hepatoma HepG2 cell, people's lung cancer cell A-549, people cancer of the stomach SGC7901 cell, human esophagus cancer KYSE109 cell and mammary cancer MDA-MB-435 cell, illustrating that this compound can as suppressing the chemicals of liver cancer, lung cancer, cancer of the stomach, the esophageal carcinoma and mammary cancer, pointing out described compound to have important application preparing in prevention and therapy liver cancer, lung cancer, the esophageal carcinoma, cancer of the stomach and breast cancer medicines.
The invention provides a kind of preparation for prevention and therapy liver cancer, wherein: described preparation contains the 16 above-mentioned carbon diacetyls for the treatment of significant quantity without double bond lactone type sophorolipid and pharmaceutically acceptable carrier.
The invention provides a kind of preparation for prevention and therapy lung cancer, wherein: described preparation contains the 16 above-mentioned carbon diacetyls for the treatment of significant quantity without double bond lactone type sophorolipid and pharmaceutically acceptable carrier.
The invention provides a kind of preparation for the prevention and therapy esophageal carcinoma, wherein: described preparation contains the 16 above-mentioned carbon diacetyls for the treatment of significant quantity without double bond lactone type sophorolipid and pharmaceutically acceptable carrier.
The invention provides a kind of preparation for prevention and therapy cancer of the stomach, wherein: described preparation contains the 16 above-mentioned carbon diacetyls for the treatment of significant quantity without double bond lactone type sophorolipid and pharmaceutically acceptable carrier.
The invention provides a kind of preparation for prevention and therapy mammary cancer, wherein: described preparation contains the 16 above-mentioned carbon diacetyls for the treatment of significant quantity without double bond lactone type sophorolipid and pharmaceutically acceptable carrier.
Above-mentioned pharmaceutically acceptable carrier refers to the pharmaceutical carrier of pharmaceutical field routine, such as: thinner is as water, ethanol etc., and weighting agent is as starch, sucrose etc., and tackiness agent is as derivatived cellulose, alginate, gelatin and polyvinylpyrrolidone; Wetting agent is as glycerine; Disintegrating agent is as agar, calcium carbonate and sodium bicarbonate; Absorption enhancer is as quaternary ammonium compound; Tensio-active agent is as cetyl alcohol; Absorption carrier is as kaolin and soap clay; Lubricant is as talcum powder, calcium stearate or magnesium, polyoxyethylene glycol etc.; Other assistant agent is as flavouring agent, sweeting agent etc.
Above-mentioned preparation is comparatively suitable for being applied to by the mode of oral, rectum or administered parenterally the patient needing described treatment for diseases.For time oral, conventional solid preparation can be made into as tablet, pulvis, granula, capsule etc., or make liquid preparation if water or oil-suspending agent or other liquid preparation are as syrup, spirit, elixir etc.; During for administered parenterally, the solution of injection, water or oleaginous suspension etc. can be made into.Preferred dosage form is tablet, coated tablet, capsule, suppository, nasal spray and injection.
Various formulation of the present invention can be prepared according to the conventional production process of pharmaceutical field, such as, make activeconstituents mix with one or more carriers, be then made into required formulation.
Preparation of the present invention preferably containing weight ratio be the activeconstituents 16 carbon diacetyl of 0.1%-99.5% without double bond lactone type sophorolipid, most preferably containing weight ratio be that the activeconstituents 16 carbon diacetyl of 5%-95% is without double bond lactone type sophorolipid.
The amount of application of the compounds of this invention can according to the age of route of administration, patient, body weight, the change such as type and severity of disease for the treatment of, and its per daily dose can be 4-50mg/kg body weight, preferred 10-50mg/kg body weight.Can use by one or many.
The Wickerhamiella domercqiae var. sophorolipid fermentative production with independent intellectual property right that 16 carbon diacetyls of the present invention are separated by contriver laboratory without double bond lactone type sophorolipid; crude product sophorolipid output can reach 150g/L; there are the potentiality that large scale fermentation is produced; and sophorolipid is formed by reproducible natural matter fermentation, very low to humans and animals toxicity.The application a few days ago, applicant had once carried out preliminary study to the crude product sophorolipid that Wickerhamiella domercqiae var. sophorolipid produces to the In-vitro Inhibitory Effect of human liver cancer cell, lung carcinoma cell, cervical cancer cell, esophageal cancer cell, the purifying of the sophorolipid molecule that perfect on this basis structure is different and preparation method, define this patent.
According to latest information, national tumor mortality ratio is 180.54/10 ten thousand, and every year because cancer mortality case reaches 2,700,000 examples, an annual new tumor cases of sending out is about 3,120,000 examples, average every day 8550 people, national per minute has 6 people to be diagnosed as cancer.Resident is 13% because of the probability of cancer mortality, namely has 1 people in every 7 to 8 people because of cancer death.Although the change in more first 20 years of present M & M is little, data presentation, China's pathogenesis of cancer presents rejuvenation trend, comprises the age of onset such as mammary cancer, lung cancer, colorectal carcinoma, thyroid carcinoma all lower than former age of onset.
From disease kind, occupying national malignant tumor morbidity primary is lung cancer, is secondly cancer of the stomach, colorectal cancer, liver cancer and the esophageal carcinoma.Occupy that national malignant tumor is dead is primaryly still lung cancer, be secondly liver cancer, cancer of the stomach, the esophageal carcinoma and colorectal cancer, front 10 malignant tumours account for 84.27% of whole malignant tumour.Mortality ratio soprano men and women is lung cancer.
Although cancer has a strong impact on the physical and mental health of people, the treatment at present for cancer does not have specific method, mainly carries out surgical operation, radiation and chemotherapy in early days.The methods for the treatment of of terminal cancer mainly contains chemicotherapy and Chinese medicine, and along with treatment time extends, the toxic side effect brought is also larger.Therefore, development of new anticarcinogen is imperative.Experiment prompting, 16 carbon diacetyls provided by the invention are the anti-kinds of tumors of preparation without double bond lactone type sophorolipid and toxic side effect is less, the more obvious antitumour drug of effect provides the foundation and approach, are extremely important.
Accompanying drawing explanation
The HPLC collection of illustrative plates of Fig. 1 sophorolipid crude extract.
Fig. 2 electron microscopic observation 16 carbon diacetyl (is called for short: after C16:0DLSL) acting on HepG2, nucleus changes without double bond lactone type sophorolipid
Wherein: the normal tumour cell of A; Tumour cell after B 60 μ g/ml sophorolipid (C16:0DLSL) effect;
After Fig. 3 Flow Cytometry detects C16:0DLSL effect, the cell cycle changes
Wherein: the normal tumour cell of A; Tumour cell after B 60 μ g/ml sophorolipid (C16:0DLSL) effect; Tumour cell after the process of C 2 μ g/ml Zorubicin.
Fig. 4 TUNEL apoptosis in-situ detection reagent box detects the cell situation after C16:0DLSL effect.
Embodiment
Make those skilled in the art more fully understand the present invention below in conjunction with embodiment, but do not limit the present invention in any way.
Embodiment 1:
1. 16 carbon diacetyls are without the preparation of double bond lactone type sophorolipid
1.1 utilize Wickerhamiella domercqiae var. sophorolipid fermentative production crude product lactone type sophorolipid
(1) bacterial classification is selected: Wickerhamiella domercqiae var. sophorolipid (Wickerhamiella domercqiae var.sophorolipid) CGMCCNo.1576;
(2) slant culture: by strain inoculation in basic inorganic salt substratum, wherein adding mass volume ratio is the agar of 1.6%, under 30 DEG C of conditions, static gas wave refrigerator 36 hours;
(3) seed culture: the bacterial strain that step (2) is cultivated, aseptically containing mass volume ratio with inoculation articulating 1 ~ 2 ring in 50ml is in the substratum of the glucose of 8% and the rapeseed oil of 3%, under 30 DEG C of conditions, shaking culture 36 hours, obtained seed liquor;
(4) enlarged culturing: with the inoculum size of the volume ratio of 7%, seed liquor being inoculated in 500ml, to contain mass volume ratio be in the substratum of the rapeseed oil of 3%, under 30 DEG C of conditions, shaking culture 200 hours;
(5) lactone type sophorolipid preparation: by the extraction into ethyl acetate of the fermented liquid two volumes that step (4) obtains, upper organic phase uses n-hexane 2 times after underpressure distillation at 50 DEG C, obtain faint yellow blocks of solid through 50 DEG C of underpressure distillation again, be the lactone type sophorolipid sample slightly carried.
1.2 ten six carbon diacetyls are without the separation and purification of double bond lactone type sophorolipid
Above-mentioned lactone type sophorolipid crude extract is dissolved in Chromatographic Pure Methanol respectively, analyzes for analysis mode HPLC after 0.22 μm of organic membrane filter.Before upper prop, ultrapure water and organic solvent all will filter through 0.22 μm of filter membrane respectively, load different solvents bottle.Condition: pillar is KromasiL C18 analytical column (5 μm × 250mm × 4.6mm, Agela Technologies Inc., USA), moving phase: the gradient elution (v/v) under acetonitrile-water, elution protocol: 0 ~ 15min, ethane nitrile content is increased to 60% from 40%; 15 ~ 35min, ethane nitrile content is increased to 70% from 60%.Sampling volume: 15 μ l, flow velocity: 1ml/min, determined wavelength: 207nm.Preparation HPLC condition: pillar is PrepHT XDB C
18column (250mm × 21.2mm, Agela Technologies Inc., USA) preparative column, sampling volume: 500 μ l, flow velocity: 15ml/min, elution requirement is identical with analysis mode HPLC.The different fractions collected carries out concentrating under reduced pressure respectively, carries out structural analysis.The results are shown in Figure 1.
2. 16 carbon diacetyls are without the Structural Identification of double bond lactone type sophorolipid
2.1 MS analyze
The sophorolipid structure of 18C is divided into identify with cation mode MS/MS to fatty acid moieties.MS result shows, and [M-H]-occur in m/z 661, therefore the molecular weight of this component should be 662, infers it may is the diacetyl lactone type sophorolipid (C16:0DLSL) that fatty acid moieties is divided into C16:0.In CID-MS spectrogram, two ion m/z 619 and m/z601 lose a C after the non-reducing end glucose group open loop of sophorose part
2h
2o group or a C
2h
2o group and a water molecules formed, and ion m/z 559 may be then that the reducing end of deprotonation ion m/z 661 or non-reducing end glucosyl group lose a C
2h
4o
2with a C
2h
2o formed; Deprotonation ion m/z 661 loses AcOC
6h
9o
4the reducing end or the non-reducing end glucosyl group that form ion m/z 483, ion m/z 541 deprotonation ion m/z 661 lose two C
2h
4o
2; Ion m/z 499 loses C
6h
10o
5; Ion m/z 475 loses AcOC
6h
7o
3formed; Ion m/z 457 loses AcOC
6h
9o
4and H
2o, ion m/z 433 lose AcOC
6h
7o
3and C
2h
2o is formed.Ion m/z415 loses AcOC
6h
7o
3and C
2h
4o
2formed; Ion m/z 271 (C
16h
31o
3) be deprotonated hydroxyl group lipid acid, illustrate that this sophorolipid contains C16:0 hydroxy fatty acid.To sum up, this sophorolipid should be C16:0DLSL.
The two-dimensional spectrum analysis of 2.2 nucleus magnetic resonance
The Two-dimensional NMR Map analytical results of 16 carbon diacetyl one double bond lactone type sophorolipids is in table 1.
Structural analysis (the solvent C DCl of a table 1 16 carbon diacetyl double bond lactone type sophorolipid
3, interior mark TMS)
Embodiment 2:
Preparation method: 16 carbon diacetyls are mixed with lactose and W-Gum without double bond lactone type sophorolipid (C16:0DLSL), evenly moistening with water, sieve and drying; after sieve, add Magnesium Stearate, compressing tablet evenly; every sheet heavy 240mg, C16:0DLSL sophorolipid content is 6mg.
Embodiment 3:
Preparation method: mixed with lactose and Magnesium Stearate by C16:0DLSL, sieve, Homogeneous phase mixing in suitable container, loads hard gelatin capsule the mixture obtained, and heavy 200mg, the C16:0DLSL content of each capsule is 14mg.
Embodiment 4:
Preparation method: C16:0DLSL and sodium-chlor are dissolved in appropriate water for injection, filters gained solution and aseptically loads in ampoule.
Embodiment 5:
Preparation method: mixed with lactose and Magnesium Stearate by C16:0DLSL, sieve, Homogeneous phase mixing in suitable container, loads hard gelatin capsule the mixture obtained, and heavy 200mg, the C16:0DLSL content of each capsule is 18mg.
Embodiment 60 six carbon diacetyl is without double bond lactone type sophorolipid (C16:0DLSL) inducing apoptosis of tumour cell
Be after the C16:0DLSL effect HepG2 cell 24h of 60 μ g/ml with final concentration, tryptic digestion collecting cell, the PBS of pH 7.2 washes 2 times; The cell precipitation glutaraldehyde stationary liquid of 2.5% is fixed, and 4 DEG C are spent the night; The PBS of pH 7.2 washes 3 times; Osmic acid 4 DEG C of fixing 2h of 1%; Gradient acetone dehydration (25%-100% acetone); Embedding medium embeds; LKBV type ultramicrotome is cut into slices, and JEM-1200EX transmission electron microscope observing is also taken pictures.Result shows, normal HepG2 cell, and cell caryoplasm ratio is large, core euchromatin, and kernel is large, and nuclear membrane is complete incisura.After Compound C 16:0DLSL effect, cell volume reduces, and cytoplasm concentrates, and nuclei dyeing chromaticness aggegation engrain condenses inside nuclear membrane, and becomes block or crescent marginalisation; In endochylema, cavity increases, and cytolemma is complete, and surperficial microvillus and pseudopodium reduce; And the apoptosis body (Fig. 2) of visible film parcel nuclear particulate.Flow Cytometry shows that most of HepG2 cell block is at G after C16:0DLSL induction
1phase, small part cell block, in the S phase, makes cell can not enter the next cell cycle, thus apoptosis occurs.Compound C 16:0DLSL concentration be 60 μ g/ml induce 24h, the ratio that the hypodiploid of cell accounts for is 28.0% (Fig. 3).Collect logarithmic phase HepG2 liver cancer cell, after the C16:0DLSL effect 24h of 1.0 μMs, detect the cell situation after C16:0DLSL effect with TUNEL apoptosis in-situ detection reagent box, result display C16:0DLSL is induction of the apoptosis (Fig. 4) of HepG2 cell.
Embodiment 70 six carbon diacetyl is active without double bond lactone type sophorolipid anti-tumor in vivo
The 16 carbon diacetyls that employing embodiment 1 obtains, without double bond lactone type sophorolipid (C16:0DLSL), take nude mouse as research object, to implantation tumor cell in its body, carry out the test of sophorolipid anti-tumor in vivo.Specific experiment step is as follows:
1 experiment material:
Human liver cancer cell HepG2 cell, people's lung cancer A-549, people cancer of the stomach SGC7901, human esophagus cancer KYSE109 and mammary cancer MDA-MB-435 nude mouse, syringe, syringe needle etc.
2 experimental procedures and result
(1) inhibiting tumor assay: mouse tumor model is set up: after human liver cancer cell HepG2 cell, people's lung cancer A-549, people cancer of the stomach SGC7901, human esophagus cancer KYSE109 and mammary cancer MDA-MB-435 cell strain are recovered respectively, by every nude mouse intraperitoneal injection about 1.5 × 10
7individual viable cell.After 7 ~ 10 days, aseptically extract ascites, adjustment cell concn is 5 × 10
7individual/mL.On the right side of nude mouse, oxter about 2cm place's subcutaneous vaccination on midaxillary line is about l × 10 successively immediately
7individual viable cell.For becoming knurl during diameter of tumor >=5mm.
(2) Experiment on therapy: carry out tumor inoculation after 7 days to mouse, is divided into 5 groups at random by solid tumor mouse model animal, often organizes 10.Be respectively control group: 80% ethanolic soln; Sophorolipid low dose group 5mg/kg, middle dosage group 50mg/kg, high dose group 500mg/kg; Endoxan administration group, respectively successive administration 10 days.
(3) calculating of solid tumor mouse tumour inhibiting rate: last administration dislocates after 48 hours and puts to death mouse, takes knurl block and weighs, according to lower formulae discovery tumour inhibiting rate after weighing.
Tumor control rate (%)=[(control group knurl weight-experimental group knurl weight)/control group knurl weight] × 100%
The results are shown in Table 2.
Table 2 sophorolipid rings Nude Mice knurl ghost image
As can be seen from above data, the C16:0DLSL sophorolipid of 5mg/kgbw body weight is 16 ~ 18% for the tumour inhibiting rate of 5 kinds of human tumor cells; 50mg/kgbw sophorolipid administration group tumour inhibiting rate reaches 25 ~ 26%; 500mg/kgbw sophorolipid administration group inhibitory rate is to 34 ~ 35%, and extremely remarkable with 80% ethanolic soln control group difference, tumour inhibiting rate is less than cyclophosphamide-a control group.
(4) sophorolipid is observed the toxic side effect of tumor bearing nude mice: after treating 13 days with the C16:0DLSL of 50mg/kg body weight, claims Mouse Weight, detects sophorolipid to the impact of mouse growth, the results are shown in Table 3.
Impact on Mouse Weight after the treatment of table 3 C16:0DLSL sophorolipid
From result, after treating 13 days with C16:0DLSL, each sophorolipid experimental mice generalized case is good, feed compares no significant difference with body weight with 80% ethanolic soln group, and endoxan positive controls the 13rd day mouse after treatment starts engenders and seriously becomes thin, malnutrition, feed is passive, there is notable difference (P<0.05) between body weight change and 80% ethanolic soln group.Illustrate that sophorolipid has no side effect substantially to mouse, and endoxan is stronger to mouse toxic action.
Above-mentioned experiment shows, C16:0DLSL sophorolipid all has very strong restraining effect for human liver cancer cell HepG2 cell, people's lung cancer A-549, people cancer of the stomach SGC7901, human esophagus cancer KYSE109 and mammary cancer MDA-MB-435 cell.Resistance to malicious ability due to mouse is 11 times of people, and thus described 16 carbon diacetyls are 4-50mg/kg body weight without the drug level of the effective antitumor of double bond lactone type sophorolipid C16:0DLSL, and preferred agents concentration is 10-50mg/kg body weight.
Claims (3)
1. for a preparation for the prevention and therapy esophageal carcinoma, it is characterized in that: described preparation is made up of without double bond lactone type sophorolipid and pharmaceutically acceptable carrier 16 carbon diacetyls shown in the formula (I) for the treatment of significant quantity;
2. for a preparation for prevention and therapy cancer of the stomach, it is characterized in that: described preparation is made up of without double bond lactone type sophorolipid and pharmaceutically acceptable carrier 16 carbon diacetyls shown in the formula (I) for the treatment of significant quantity;
3. for a preparation for prevention and therapy mammary cancer, it is characterized in that: described preparation is made up of without double bond lactone type sophorolipid and pharmaceutically acceptable carrier 16 carbon diacetyls shown in the formula (I) for the treatment of significant quantity;
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2013
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| WO2011039014A1 (en) * | 2009-09-29 | 2011-04-07 | Evonik Goldschmidt Gmbh | Use of sophorolipids and derivatives thereof in combination with pesticides as adjuvant/additive for plant protection and the industrial non-crop field |
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