CN103268467A - High-throughput sample management system - Google Patents
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- CN103268467A CN103268467A CN2013102072048A CN201310207204A CN103268467A CN 103268467 A CN103268467 A CN 103268467A CN 2013102072048 A CN2013102072048 A CN 2013102072048A CN 201310207204 A CN201310207204 A CN 201310207204A CN 103268467 A CN103268467 A CN 103268467A
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Abstract
The invention discloses a high-throughput sample management system. The high-throughput sample management system comprises a grid-shaped supporting frame, wherein a test tube type container for containing a sample is arranged in each grid of the supporting frame. The high-throughput sample management system is characterized in that a unique two-dimension code corresponding to each container is arranged at the bottom of each container. Compared with an existing work mode, by mean of the high-throughput sample management system, a user omits procedures of coding, bar code printing, bar code bonding, bar code checking and the like. Due to the fact that the sizes and arrangement of the containers can be determined according to industrial standards, split packaging of the samples can be carried out by utilizing automatic equipment or batch processing equipment. In the process of recognition, the method that the bar codes of the containers are read one by one is not needed, and the bar codes of a hundred or hundreds of the containers can be read each time through a scanner. Compared with the existing work mode, the high-throughput sample management system improves the efficiency by hundreds of times.
Description
Technical field
The present invention relates to container and management system thereof with two-dimension code.
Background technology
Along with development of science and technology, Biological Genetic Resources being excavated and utilize by the modern biotechnology means becomes a kind of main flow and trend.In order to support life science scientific research and application and development, a kind of novel scientific research infrastructure--living resources center (Bioresource Centers) vigorously builds in countries in the world, in order to preserve living cells, the organism genome, educable biosome (microorganism for example, plant, animal and human cell) and reproducible composition (genome for example wherein, plasmid, virus, cDNA), the organism that activity is arranged but can not cultivate, cell and tissue, and the molecule that contains these gleanings, the database of physiology and structural information and associated biomolecule information science.
Living resources are of a great variety, enormous amount.Be example with human diseases sample storehouse only, it is collected the material of preserving and comprises multiple heterogeneities such as tissue, blood, body fluid, saliva and inhereditary material, and it is hundreds thousand of that its sample size can reach easily, even millions of.At this situation, information-based means management magnanimity sample is generally adopted at the living resources center, in order to ensure each sample is carried out effective recognition and differentiation, and need be to the sample typing of encoding one by one.Mainly containing of using in practice printed bar code and used two kinds of different implementations of prefabricated bar code, but this dual mode in use all comes with some shortcomings:
Using under the working method of printing bar code, because the types and sources difference of sample, its initial numbering is different.Operation each time in the processing links all needs to set up prints bar code and initial number related, and is pasted on the tube outer wall by artificial mode.The label coding mode that this needs are manually printed, paste, checked, it is simply uninteresting to work, and brings out fatigue easily and causes operating personnel faulty operation to occur.
Though use prefabricated bar code can simplify a large amount of artificial duplication of labour, raise the efficiency.But it is little that preform strip code book body and sample are initially numbered the degree of association, and operating personnel need to carry out related to coding with sample one by one.Along with the increase of sample preparation quantity, the needs of the equal incompatible extensive sample high-level efficiency coding of above-mentioned dual mode and management press for a kind of new method and technology and address the above problem.
Summary of the invention
At above-mentioned drawback, the present invention is on generally accepted two-dimension code application foundation, design one cover comprises the solution of container, equipment and management software, realized sample encoded is carried out the function of batch identification and management, and then making the efficient of sample managing and reliability become hundred times lifting, the present invention also is applicable to the automation equipment use.
Technical scheme of the present invention provides a kind of high flux sample managing system, by tubular container, grid-shaped bracing frame, and scanner and control software composition, it is characterized in that: described container bottom is provided with and the unique corresponding two-dimension code of this container, the grid-shaped support does not have the end, the some tubular containers that are locked on the bracing frame obtain bottom image by scanner, and by two bit codes of controlling software parsing container bottom and related with its position.
Preferably, one jiao of shortcoming of support frame as described above, this shortcoming position is corresponding to the coordinate-system initial point of support, the design of grid-shaped support has locking mechanism, can with the respective outer side edges of tubular container bottom, in order to tubular container is fixed on the support, and the bottom of all tubular containers is positioned on the same surface level, support frame as described above bottom and sidewall are provided with and the unique corresponding two-dimension code of support frame as described above or one dimension sign indicating number.
Preferably, this scanner uses the CCD element to obtain image, lens by being positioned at CCD element top in 0 ~ 3.5mm place, top, sheet glass plane of placing grid-shaped support and tubular container, or make the optimal imaging range regulation it be close to by thickening sheet glass thickness to realize bottom the tubular container.
Preferably, described scanner comprises the cover plate that opening is arranged on, and openings of sizes is consistent with the grid-shaped support, and its opening is corner cut for one jiao, guarantees that net grid support puts into correct direction; Described cover plate one side is provided with glass plate, one litter is set on the described cover plate movably, can the one scan head be set movably along described litter on the described litter, described scanner also comprises and drives first driving mechanism that described scanner head moves along described litter and drive second driving mechanism that described litter moves along described cover plate.
Preferably, described first driving mechanism and second driving mechanism are stepper motor or cylinder.
Preferably, described scanner head comprises light source, stripe-shape plane catoptron, condenser lens and CCD charge-coupled image sensor.
Preferably, described container bottom is circular, and its diameter is d, and the step that described image processing module obtains described two-dimension code comprises:
1) image pre-service is carried out filtering and binary conversion treatment to described digital picture, and described digital picture is changed into 16 color shade images;
2) calculation procedure 1) whole gray values of pixel points An in the 16 color shade images that obtain;
3) set up coordinate system, utilize the sobel operator to calculate the shade of gray of each pixel and neighbor, wherein:
The shade of gray Gx=An*[-1-2 1 of X-direction; 000; 12 1];
The shade of gray Gy=An*[-1 01 of Y direction;-201;-10 1];
4) if the shade of gray Gx of arbitrary pixel, Gy is the center of circle with this pixel then all greater than predefined first threshold, is radius with 0.7d, calculates all shade of gray Gx in this zone, Gy is all greater than the number of the point of predefined first threshold; As the number that calculates is less than predefined second threshold value, and then this pixel is noise spot; Otherwise then this pixel is available point;
5) calculation procedure 4) in the median on X-axis and Y-axis respectively of whole available point of obtaining, be that the point of coordinate is the center of circle with the median on X-axis and the Y-axis, be radius with 0.7d, repeating step 3), 4) calculate the coordinate of available points whole in this circle;
6) median of whole available points on X-axis and Y-axis to obtain in the previous step is that the point of coordinate is the center of circle with the median on X-axis and the Y-axis, is radius with 0.7d, repeating step 3), 4) calculate the coordinate of available points whole in this circle;
7) repeating step 6) identical with last result of calculation until result of calculation, be described two-dimension code with the whole available points that calculate for the last time.
Preferably, the digital picture of the whole described container bottoms of described scanner collection and bracing frame bottom.
Preferably, further comprising the steps of before the described step 1):
0) according to the size of mesh opening of support frame as described above, the whole described container bottom of described scanner collection and the digital picture of bracing frame bottom are divided into the little digital picture that several only comprise a described grid, with the input of this little digital picture as described image processing module.
Preferably, described scanner is also gathered the two-dimension code of support frame as described above bottom.By software, carry out all container number and the position of placing on the numbering of bracing frame and the bracing frame related.
High flux sample managing system of the present invention is than the work on hand mode, and the user has omitted coding, prints bar code, pastes bar code, checks steps such as bar code.Because container dimensional and arrangement can be followed industrial standard, can use automation equipment or batch processing equipment to carry out the sample packing.When identification, do not need to read one by one the bar code of each container, can once read the bar code of up to a hundred and even hundreds of containers by the scanner of special use.In use, only the coding on the needs identification bracing frame can obtain position and the coded message of all containers on this bracing frame, compares the work on hand mode, and efficient has improved hundreds of times.
Description of drawings
Fig. 1 is system architecture synoptic diagram of the present invention;
Fig. 2 is the structural representation of container and the two-dimension code synoptic diagram of bottom thereof;
Fig. 3 is the structural representation of scanner;
Fig. 4 is the structural representation of scanner head;
Fig. 5 is the workflow diagram of image processing module.
Embodiment
Below the specific embodiment of the present invention is described in further detail.
Extremely shown in Figure 5 as Fig. 1, high flux sample managing system of the present invention is taked following technical solution: a kind of bottom has two-dimensional encoded container 100, hold the bracing frame 200 of two-dimensional bar code container and form based on the scanner of linear array CCD scanning head and the control decoding software that runs on main frame, wherein control decoding software and comprise image processing module and decoder module.
The bottom of container 100 is taper type, is marked with two bar codes with the Datamatrix standard by means such as printing, stickup or laser-induced thermal etchings on its bottom end face.The bar code minimum is the 10*10 module, and the physical size of forming module is not less than 0.3mm*0.3mm, can increase module number or module area under container 100 bottom area enabled conditions.
When placing container 100 on the bracing frame 200, the vertical height on the plane of the bottom two-dimension code plan range placement bracing frame 200 of bracing frame 200 upper containers 100 is between 0.3mm～2mm.
Scanner head 301 is fixed in and supports on the litter 302, supports litter 302 and is driven by stepper motor 303, makes scanner head 301 obtain the two-dimensional barcode image that is placed on glass platform 304 all container bottoms of top in the mode of lining by line scan.This structure is not innovative point of the present invention place, and the various scanner structure that industry has adopted all may be used on realizing corresponding function among the present invention.
Be stamped the cover plate (not shown) of hollow on the clear glass platform 304 of scanner 300, the zone of cover plate hollow is just in time identical and more bigger than bracing frame with bracing frame 200 shapes, bracing frame can be limited in the specific scope.
Scanner obtains the two-dimensional barcode image of all container 100 bottoms that are positioned on the support and the image in 2 D code on the support with the resolution that is not less than 300dpi.The image that obtains input control decoding software, by the software computing that positions and decode.
Scanner head is made up of light source 306 (bar shaped fluorescent tube), stripe-shape plane catoptron (not shown), condenser lens 307 (lens combination) and CCD charge-coupled image sensor 305.Be stamped clear glass platform 304 on the scanner head, by adjusting the lens on the scanner head, make the best depth of focus of scanner head imaging be positioned at glass platform 304 tops 0～3.5mm scope.
Obtain after the image, the control decoding software can carry out following work:
The pre-service of picture, image processing module change into 16 color shades with the picture that obtains after at first image being carried out filtering and binary conversion treatment.
Cutting apart of container bottom two-dimension code zone, scanning has obtained a large amount of container bottom two-dimension codes, at first need these two-dimension code zones are cut apart, simultaneously two-dimension code zone and the coordinate of position are carried out relatedly, and then one by one two-dimension code is carried out in the two-dimension code zone after cutting apart and identify.
The two-dimension code zone is identified, is used the sobel operator to calculate, be implemented as follows:
1) calculates the shade of gray of each pixel and neighbor successively.The gray-scale value of picture pixel is An, and then the calculated value of X-direction is Gx=An*[-1-2 1; 000; 12 1], the calculated value of Y direction is Gy=An*[-1 01;-201;-10 1].As Gx, Gy then charges to buffer memory with the coordinate figure of this pixel all greater than predetermined threshold value.
2) be the center of circle with the pixel coordinate of charging to buffer memory, judge this pixel 0.7d radius region interior (d is the container bottom diameter) on every side, whether the number of the pixel of all satisfy condition (as Gx, Gy is all greater than predetermined threshold value) is greater than pre-set threshold.As less than threshold value, judge that then this pixel is noise spot, this pixel of deletion from buffer memory; As greater than threshold value, then enter next step.
3) median of all pixel coordinates in the calculating buffer memory as the new center of circle, with 0.7d radius (d is the container bottom diameter), calculates the median of all qualified pixel coordinates in institute's coverage with this coordinate, and as the new center of circle.
4) calculating repeating step 3 then), until result of calculation with last time result of calculation was identical.This moment, the pixel coordinate that obtains can be regarded the center of two-dimensional bar code as, cut with the 0.7d radius.Obtain independent two-dimension code and be used for decoding calculating.Above step can simply and exactly be determined the pixel of two-dimension code, for thereafter decoding provides safeguard.
5) two-dimensional bar code is decoded.Because this partial content elaborates in multinomial patent and document, so do not describe in detail at this; For example, Chinese patent 201010195178.8.In short, by surveying " L " type limit of Datamatrix bar code, obtain relevant bar code scope according to " L " type limit then, module information is also decoded.
In decoding software, with the container position information of middle calculating gained with in decoded bar code information carry out relatedly, set up the ad-hoc location information related with bar code.
Bar code on the bracing frame can be by the decoding of common barcode scanner, and encodes with the container on the bracing frame and the position is associated by decoding software; The two-dimensional bar code of bracing frame bottom can be by 1 among the present invention)-4) step obtains, and encodes and location association by decoding software and container.
For making those skilled in the art be easier to understand technology contents of the present invention, the reference and explanation of following bar code term are provided, wherein part bar code term is to come according to State Standard of the People's Republic of China B/T12905-2000, is not for the present invention is limited.
Module: a smallest standalone unit in the matrix two-dimensional barcode represents a bit binary data.
Pixel: the single photosensitive unit corresponding unit on bar code image on the light-sensitive array (as CCD or CMOS device), a pixel is regarded as the complete unit of the minimum of bar code image usually.The storing mode of digital bar code image is unit with pixel (Pixel) generally, and each pixel is the long-pending minimum unit in digital bar code image the inside.
Data Matrix sign indicating number also claims data matrix code, comprises two types: ECC000-140 and ECC200.The research of Data Matrix sign indicating number at present and application are mainly based on ECC200, and CC000-140 uses seldom.The functional graphic of Data Matrix sign indicating number comprises position sensing figure and correction graph.The code area comprises version information and the format information district that represents symbolic information, and code word data and error correcting code block.The symbolic coding of ECC200Data Matrix sign indicating number does not adopt mask graph, its error correction is related with the module arrangement mode, therefore the zone of symbolic information is not represented in the code area of ECC200Data Matrix sign indicating number, include only code word data and error correcting code block, according to the module pixel coordinate of detected ECC200Data Matrix sign indicating number, namely can determine module parameter (module size, line number, columns etc.) and the error-correction level of ECC200Data Matrix sign indicating number.
The position sensing figure of Data Matrix sign indicating number is to be positioned at the zone that the data area border width is 1 module.The left side of Data Matrix sign indicating number and following two adjacent limits are dark solid line, have formed a L shape border, are mainly used in determining physical size (being the bar code zone), location and the correcting distortion of bar code.The correction graph that two opposite side on L shape border are made up of dark color and the light module of the individual module width that replaces is mainly used in limiting module coordinate and the correcting distortion of bar code, but also can assist the physical size of determining bar code.
The abbreviation of DPI:Dots Per Inch, what per inch was printed counts or the line number.
CCD: English full name: Charge-coupled Device, Chinese full name: charge coupled cell.CCD is a kind of semiconductor devices, can be converted into digital signal to optical image.
During use, the shelf of coding container and placement coding container is placed on the ccd scanner, starts whole image in 2 D code that scanner obtains container and bottom shelf.
The operation decoding software is decoded the two-dimensional bar code of all containers on the shelf and corresponding its position coordinates successively.
Obtain the coded message on the shelf, the shelf coded message is related with container two-dimension code and positional information, enter the information management system that the user uses.
When the user deposits sample, only need deposit sample successively according to the coded sequence on the shelf, and the position of the type of sample and information and sample related (this step can be finished by automation equipment) successively.In infosystem, can finish the coding association of sample packing front and back.
Above embodiment only is the present invention's a kind of embodiment wherein, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. high flux sample managing system, by tubular container, grid-shaped bracing frame, and scanner and control software composition, it is characterized in that: described container bottom is provided with and the unique corresponding two-dimension code of this container, the grid-shaped support does not have the end, the some tubular containers that are locked on the bracing frame obtain bottom image by scanner, and by two bit codes of controlling software parsing container bottom and related with its position.
2. high flux sample managing system according to claim 1, it is characterized in that: one jiao of shortcoming of support frame as described above, this shortcoming position is corresponding to the coordinate-system initial point of support, the design of grid-shaped support has locking mechanism, can with the respective outer side edges of tubular container bottom, tubular container is fixed on the support, and the bottom of all tubular containers is positioned on the same surface level, support frame as described above bottom and sidewall are provided with and the unique corresponding two-dimension code of support frame as described above or one dimension sign indicating number.
3. high flux sample managing system according to claim 2, it is characterized in that: this scanner uses the CCD element to obtain image, lens by being positioned at CCD element top in 0 ~ 3.5mm place, top, sheet glass plane of placing grid-shaped support and tubular container, or make the optimal imaging range regulation it be close to by thickening sheet glass thickness to realize bottom the tubular container.
4. high flux sample managing system according to claim 3, it is characterized in that: described scanner comprises the cover plate that opening is arranged on, and openings of sizes is consistent with the grid-shaped support, and its opening is corner cut for one jiao, guarantees that net grid support puts into correct direction; Described cover plate one side is provided with glass plate, one litter is set on the described cover plate movably, can the one scan head be set movably along described litter on the described litter, described scanner also comprises and drives first driving mechanism that described scanner head moves along described litter and drive second driving mechanism that described litter moves along described cover plate.
5. high flux sample managing system according to claim 4, it is characterized in that: described first driving mechanism and second driving mechanism are stepper motor or cylinder.
6. high flux sample managing system according to claim 5, it is characterized in that: described scanner head comprises light source, stripe-shape plane catoptron, condenser lens and CCD charge-coupled image sensor.
7. according to one of them described high flux sample managing system of claim 3-6, it is characterized in that: described container bottom is for circular, and its diameter is d, and the step that described image processing module obtains described two-dimension code comprises:
1) image pre-service is carried out filtering and binary conversion treatment to described digital picture, and described digital picture is changed into 16 color shade images;
2) calculation procedure 1) whole gray values of pixel points An in the 16 color shade images that obtain;
3) set up coordinate system, utilize the sobel operator to calculate the shade of gray of each pixel and neighbor, wherein:
The shade of gray Gx=An*[-1-2 1 of X-direction; 000; 12 1];
The shade of gray Gy=An*[-1 01 of Y direction;-201;-10 1];
4) if the shade of gray Gx of arbitrary pixel, Gy is the center of circle with this pixel then all greater than predefined first threshold, is radius with 0.7d, calculates all shade of gray Gx in this zone, Gy is all greater than the number of the point of predefined first threshold; As the number that calculates is less than predefined second threshold value, and then this pixel is noise spot; Otherwise then this pixel is available point;
5) calculation procedure 4) in the median on X-axis and Y-axis respectively of whole available point of obtaining, be that the point of coordinate is the center of circle with the median on X-axis and the Y-axis, be radius with 0.7d, repeating step 3), 4) calculate the coordinate of available points whole in this circle;
6) median of whole available points on X-axis and Y-axis to obtain in the previous step is that the point of coordinate is the center of circle with the median on X-axis and the Y-axis, is radius with 0.7d, repeating step 3), 4) calculate the coordinate of available points whole in this circle;
7) repeating step 6) identical with last result of calculation until result of calculation, be described two-dimension code with the whole available points that calculate for the last time.
8. high flux sample managing system according to claim 7 is characterized in that: the digital picture of the whole described container bottoms of described scanner collection and bracing frame bottom.
9. high flux sample managing system according to claim 8 is characterized in that: further comprising the steps of before the described step 1):
0) according to the size of mesh opening of support frame as described above, the whole described container bottom of described scanner collection and the digital picture of bracing frame bottom are divided into the little digital picture that several only comprise a described grid, with the input of this little digital picture as described image processing module.
10. high flux sample managing system according to claim 8 is characterized in that: described scanner is also gathered the two-dimension code of support frame as described above bottom.
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| CN106022425A (en) * | 2016-05-15 | 2016-10-12 | 上海思岭信息科技有限公司 | Layered-structure 2D code encoding and decoding method |
| CN111611822A (en) * | 2020-05-14 | 2020-09-01 | 科大讯飞股份有限公司 | Information storage method, device and system, electronic equipment and storage medium |
| CN112507747A (en) * | 2020-12-10 | 2021-03-16 | 北京爱创科技股份有限公司 | Tracing code scanning device and method |
| CN112946306A (en) * | 2021-02-02 | 2021-06-11 | 北京和众视野科技有限公司 | Textile pH value full-flow testing system and testing method thereof |
| CN114013834A (en) * | 2021-07-07 | 2022-02-08 | 福宁虹昇科技(杭州)有限公司 | Automatic transfer tray |
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| CN106022425A (en) * | 2016-05-15 | 2016-10-12 | 上海思岭信息科技有限公司 | Layered-structure 2D code encoding and decoding method |
| CN106022425B (en) * | 2016-05-15 | 2019-09-10 | 杭州荣旗科技有限公司 | A kind of coding and interpretation method of layered structure two dimensional code |
| CN111611822A (en) * | 2020-05-14 | 2020-09-01 | 科大讯飞股份有限公司 | Information storage method, device and system, electronic equipment and storage medium |
| CN112507747A (en) * | 2020-12-10 | 2021-03-16 | 北京爱创科技股份有限公司 | Tracing code scanning device and method |
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| CN112946306A (en) * | 2021-02-02 | 2021-06-11 | 北京和众视野科技有限公司 | Textile pH value full-flow testing system and testing method thereof |
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