CN103263432B - Traditional Chinese medicine monomer compound preparation for preventing and treating diabetic heart injuries and preparation method thereof - Google Patents
Traditional Chinese medicine monomer compound preparation for preventing and treating diabetic heart injuries and preparation method thereof Download PDFInfo
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- CN103263432B CN103263432B CN201310233021.3A CN201310233021A CN103263432B CN 103263432 B CN103263432 B CN 103263432B CN 201310233021 A CN201310233021 A CN 201310233021A CN 103263432 B CN103263432 B CN 103263432B
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- 239000000178 monomer Substances 0.000 title claims abstract description 106
- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 37
- 150000001875 compounds Chemical class 0.000 title claims abstract description 32
- 239000003814 drug Substances 0.000 title claims abstract description 28
- 208000013875 Heart injury Diseases 0.000 title abstract 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims abstract description 54
- 241000219051 Fagopyrum Species 0.000 claims abstract description 40
- 235000009419 Fagopyrum esculentum Nutrition 0.000 claims abstract description 40
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 claims abstract description 33
- 229960004555 rutoside Drugs 0.000 claims abstract description 33
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 claims abstract description 32
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 claims abstract description 32
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 claims abstract description 32
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 claims abstract description 32
- 235000005493 rutin Nutrition 0.000 claims abstract description 32
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims abstract description 27
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims abstract description 27
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims abstract description 27
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims abstract description 27
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims abstract description 27
- 235000005875 quercetin Nutrition 0.000 claims abstract description 27
- 229960001285 quercetin Drugs 0.000 claims abstract description 27
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- 235000021283 resveratrol Nutrition 0.000 claims abstract description 27
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- 239000002253 acid Substances 0.000 claims description 4
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 claims description 4
- 229930003944 flavone Natural products 0.000 claims description 4
- 150000002212 flavone derivatives Chemical class 0.000 claims description 4
- 235000011949 flavones Nutrition 0.000 claims description 4
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 2
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000003449 preventive effect Effects 0.000 description 4
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- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
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- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 2
- 229960001052 streptozocin Drugs 0.000 description 2
- 208000021479 Cardiovascular injury Diseases 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention relates to a traditional Chinese medicine monomer compound preparation for preventing and treating diabetic heart injuries, and a preparation method thereof and belongs to the technical field of traditional Chinese medicine monomer compound preparations. According to the technical scheme, a quercetin monomer, a resveratrol monomer and an extracted powdery buckwheat rutin monomer are matched and prepared into the compound preparation; and the contentsof quercetin, resveratrol and buckwheat rutin, in percentage by mass, are 10-20%, 10-30% and 60-70% respectively. According to the compound preparation and the preparation method disclosed by the invention, raw material resources are abundant, special instrument and equipment are not needed, the cost is low, and application is convenient; by combination of the quercetin monomer with very high purity, the resveratrol monomer with a very high purity, and the rutin monomer extracted and separated from buckwheat flower leaves by a proper solvent, the multiple effects of traditional Chinese medicine are favourably exerted, the theory of traditional Chinese medicine is met, the multi-part and multi-target action mechanism of traditional Chinese medicine is embodied, and the effects are obvious and safe; and the compound preparation disclosed by the invention has a protection effect on the heart (myocardium and heart capillaries) injuries of diabetic rats, has no toxic or side effects in animal experiments, and has the characteristics of high curative effect, low dose, convenience in applicaiton and the like.
Description
Technical field
The present invention relates to a kind of Compound Chinese Herbal Monomer Recipe preparation and preparation method of preventing and treating diabetic heart damage, especially for preventing and treating Compound Chinese Herbal Monomer Recipe preparation of diabetic cardiomyopathy and heart microvascular disease and preparation method thereof, belong to Compound Chinese Herbal Monomer Recipe preparation technique field.
Background technology
At present, prevent and treat diabetic heart damage and mainly take medicine as main, mostly be medication even throughout one's life for a long time.Doctor trained in Western medicine oral antidiabetic drug or insulinize prolonged application have the problems such as compliance is poor, financial burden heavy, untoward reaction is many.Especially to reducing the diabetes heart
fleshand cardiovascular injury there is no desirable medicine and effective measures.This disease of Chinese medicine has some superiority, its action temperature and lasting.One of characteristic of middle medical drugs is compound recipe medication, by derive from different raw material form compound recipe " effective component group " for clinical, not only embodied TCM Features, and improved technology content, be also to realize the modernization of Chinese medicine and international needs.At present, the research of material foundation of tcm has become " bottleneck " in modernization of Chinese medicine process, studies the treatment that effective Chinese medicine monomer composition is applied to refractory disease and seems particularly urgent.Can make each monomer effective ingredient play a role from different perspectives, the integrally-regulated advantage of outstanding compound recipe, by plurality of active ingredients, in human body too many levels, integration regulating action multi-level, many target spots, be good at from integral body and have in mind, not only consider to control diabetic symptom, reduce blood glucose, also take disease and prognosis thereof into account, compare with current Western medicine, have irreplaceable superiority.Background technology is not is not still prevented and treated the Compound Chinese Herbal Monomer Recipe preparation of diabetic heart damage.
Summary of the invention
The object of this invention is to provide a kind of Compound Chinese Herbal Monomer Recipe preparation and preparation method of preventing and treating diabetic heart damage, curative effect is clear and definite, and consumption is little, with low cost, and taking convenience solves the problems referred to above that exist in background technology.
Technical scheme of the present invention is:
Compound Chinese Herbal Monomer Recipe preparation and a preparation method of preventing and treating diabetic heart damage, preparation process is as follows: Quercetin monomer and the purity > 98% resveratrol monomer of 1. first getting purity > 98% ready; 2. extract buckwheat rutin monomer, by the flowers and leaves of buckwheat of natural drying (from Tongliao, Inner Mongolia city coulomb flag), it is crude drug, by adding acid chitosan separating medium extraction, extract after buckwheat total flavone, then extract buckwheat rutin monomer with ethanol alcohol extracting method, the buckwheat rutin monomer of extraction is Powdered; 3. by Quercetin monomer, the Powdered buckwheat rutin monomer of resveratrol monomer and extraction is mixed in proportion, make the Compound Chinese Herbal Monomer Recipe preparation of preventing and treating diabetic heart damage, the mass percent of Quercetin monomer, resveratrol monomer and buckwheat rutin monomer is respectively: 10-20%, 10-30% and 60-70%.
The described Compound Chinese Herbal Monomer Recipe preparation of preventing and treating diabetic heart damage, is prepared into capsule, for the control of diabetic heart damage.
A kind of Compound Chinese Herbal Monomer Recipe preparation of preventing and treating diabetic heart damage, the Powdered buckwheat rutin monomer compatibility of Quercetin monomer, resveratrol monomer and extraction is prepared into compound preparation, Quercetin monomer, resveratrol monomer and buckwheat rutin monomer are Powdered, and their mass percent is respectively: Quercetin monomer 10-20%, resveratrol monomer 10-30% and buckwheat rutin monomer 60-70%; The purity > 98% of Quercetin monomer, the purity > 98% of resveratrol monomer; Buckwheat rutin monomer is using flowers and leaves of buckwheat as crude drug, extracts after buckwheat total flavone, then extract buckwheat rutin monomer with ethanol alcohol extracting method by adding acid chitosan separating medium extraction.
Compound preparation of the present invention is applied to diabetes rat, observes its antidiabetic effect and cardiac muscle, the effect of heart microvascular injury protection, result shows that the present invention can reduce diabetes rat fasting glucose and the heavy index of the heart, improves cardiac function simultaneously.To myocardial damage factor P38 α-mitogen activated protein kinase (P38 α-MAPK) protein expression, and heart microvascular damage factor monocyte chemoattractant protein-1 (MCP-1) protein expression is all had to certain inhibitory action.The ANOMALOUS VARIATIONS that can significantly improve cardiac muscle and heart microvascular organizational structure, toxicity test is not found any toxic reaction.The present invention has the features such as curative effect is high, dosing is little, application is convenient.
Good effect of the present invention is: (1) raw material resources are abundant, Quercetin monomer and resveratrol monomer source is wide, purity is high, easily obtain; (2) buckwheat rutin monomer extraction and preparation technique is simple, and method technological process is simple, does not need specific apparatus and equipment, and cost is low; (3) application is convenient, and quality controllable, the present invention, by Quercetin and very high monomer and the flowers and leaves of buckwheat of two purity of resveratrol are isolated to the combination of rutoside monomer with suitable solvent extraction, is conducive to bring into play the pleiotropy of Chinese medicine.Extraction of rutin wherein, the repeatability of quality control improves; Meet theory of Chinese medical science, embodied again the mechanism of action of Chinese medicine multiple location, many target spots.(4) successful, safety, to diabetes rat heart (cardiac muscle, heart microvascular), damage has protective effect, and toxicity is not found in zoopery in the present invention.
The specific embodiment
By the following examples, the invention will be further described.
Monomer compound preparation prepared by the present invention carries out various zoopery indexs and detects, and all obtains consistent result, and to diabetic heart, damage has preventive and therapeutic effect.To diabetic heart, damage has no preventive and therapeutic effect to the monomer compound preparation that surpasses or prepare lower than invention scope.
Embodiment mono-: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 10%, 30% and 60%.Embodiment bis-: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 15%, 20% and 65%.Embodiment tri-: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 20%, 10% and 70%.Embodiment tetra-: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 5%, 40% and 55%.Embodiment five: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 20%, 5% and 75%.Embodiment six: Quercetin monomer, resveratrol monomer and the shared mass percent of buckwheat rutin monomer three are respectively: 25%, 10% and 65%.
The monomer compound preparation of each embodiment is capsule, preparation technology is as follows: Quercetin monomer, resveratrol monomer and three kinds of compositions of buckwheat rutin monomer are compatibility according to the above ratio, and adding dextrin is excipient, makes capsule, with above-described embodiment, carry out zoopery checking, method is as follows:
embodiment zoopery
1. experiment material
1.1 laboratory animal: male SD rat, body weight 150-200g(Beijing Institute of Experimental Animals, Chinese Academy of Medical Sciences provides), the quality certification number is SCXK11-00-0006.Feeding environment: room temperature (24 scholar 2) ℃.
reagent:quercetin monomer (purity > 98%, Sigma company); Resveratrol monomer (purity > 98%, Sigma company).Streptozotocin (U.S. Sigma company), FBG test kit (Bioengineering Research Institute is built up in Nanjing), P38 α-MAPK and MCP-1 radioimmunoassay, RIA medicine box (Chinese atomic energy science institute Isotope Research institute), diabetes pill (Guangzhou Zhongyi Medicine Industry Co., Ltd, lot number: 07100213).
instrument:blood glucose meter (Korea S All Medicus company), 722 type spectrophotometers (Beijing Liuyi Instrument Factory), HP-5500 colorful ultrasonic Doppler instrument (U.S. Parkson company), the many probes of FT630G type microcomputer are put and are exempted from instrument (Beijing nuclear instrument factory)
,general refrigerator (Frestech company), ultra cold storage freezer (Therom Electron Corpation company), centrifuge (Jiangsu altar comet assay Instrument Ltd.).
experimental technique
75 of clean level male SD rats, take out 12 conducts at random
normal group, all the other add low dose of streptozotocin lumbar injection by high fat high caloric diet, set up rat diabetes model.By rejecting into 3 of the failed and dead rats of mould, one-tenth mould rat is divided into 5 groups (n=12) at random,
mono-～six group of embodiment(0.09gkg
-1d
-1),
positive control drug diabetes pill(0.83gkg
-1d
-1)
groupwith
model control group, difference gastric infusion, Normal group and model group gavage same volume normal saline, continuous 8 weeks every day.
experimental result
?table 1-4. shows embodiment mono-in invention scope
～tri-preparation can reduce diabetes abdomen blood glucose and the heavy index of the heart, improves cardiac function simultaneously.Suppress the expression of myocardium P38 α-MAPK and cardiovascular CCL2, alleviate rat heart pathology damage, diabetes rat heart and injury is had to significant protective effect, close with positive control drug.Exceed or lower than the embodiment tetra-that shows invention scope
～sixpreparation has no the protective effect to diabetes rat heart and injury.
Table 1. embodiment preparation is on the impact of diabetes rat FBG and HWI (± s, n=12)
bp<0.01
vs.normal group
cp<0.05
dp<0.01
vs.model group
Table 2. embodiment preparation is on the impact of diabetes rat cardiac function (± s, n=12)
?
ap<0.05
bp<0.01
vs.normal group
cp<0.05
dp<0.01
vs.model group
The expression impact (± s, n=12) of table 3. embodiment preparation on diabetes rat P38 α-MAPK and CCL2
bp<0.01
vs.normal group
cp<0.05
dp<0.01
vs.model group
Table 4. embodiment preparation is to diabetes rat heart tissue pathology integration (n=12)
bp<0.01
vs.normal group
cp<0.05
dp<0.01
vs.model group
The present invention, by a large amount of animal experimental observation, has obtained good effect, draws to draw a conclusion:
1. the invention solves some technical problems of Compound Chinese Herbal Monomer Recipe preparation and preparation method, in compound Chinese medicinal preparation technical field, there is the large meaning of wanting.
2. the Compound Chinese Herbal Monomer Recipe preparation in invention scope of the present invention has obvious protection diabetic heart damage (cardiac muscle and cardiovascular) effect; to diabetic heart, damage has good preventive and therapeutic effect, can be used for the control medicine of diabetes and heart and injury aspect complication thereof.
3. exceed or lower than the Compound Chinese Herbal Monomer Recipe preparation of invention scope of the present invention, diabetic heart damage (cardiac muscle and cardiovascular) had no to preventive and therapeutic effect.
Claims (2)
1. prevent and treat the Compound Chinese Herbal Monomer Recipe preparation that diabetic heart is damaged for one kind, it is characterized in that the Powdered buckwheat rutin monomer compatibility of Quercetin monomer, resveratrol monomer and extraction to be prepared into compound preparation, Quercetin monomer, resveratrol monomer and buckwheat rutin monomer are Powdered, and their mass percent is respectively: Quercetin monomer 10-20%, resveratrol monomer 10-30% and buckwheat rutin monomer 60-70%; The purity > 98% of Quercetin monomer, the purity > 98% of resveratrol monomer; Buckwheat rutin monomer is using flowers and leaves of buckwheat as crude drug, extracts after buckwheat total flavone, then extract buckwheat rutin monomer with ethanol alcohol extracting method by adding acid chitosan separating medium extraction.
2. a preparation method of preventing and treating the Compound Chinese Herbal Monomer Recipe preparation of diabetic heart damage, is characterized in that preparation process is as follows: Quercetin monomer and the purity > 98% resveratrol monomer of 1. first getting purity > 98% ready; 2. extracting buckwheat rutin monomer, is crude drug by the flowers and leaves of buckwheat of natural drying, extracts after buckwheat total flavone, then extract buckwheat rutin monomer with ethanol alcohol extracting method by adding acid chitosan separating medium extraction, and the buckwheat rutin monomer of extraction is Powdered; 3. by Quercetin monomer, the Powdered buckwheat rutin monomer of resveratrol monomer and extraction is mixed in proportion, make the Compound Chinese Herbal Monomer Recipe preparation of preventing and treating diabetic heart damage, the mass percent of Quercetin monomer, resveratrol monomer and buckwheat rutin monomer is respectively: 10-20%, 10-30% and 60-70%.
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| CN108420816A (en) * | 2018-06-07 | 2018-08-21 | 华北理工大学 | A kind of Compound Chinese Herbal Monomer Recipe preparation and preparation method thereof of prevention diabetic heart damage |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TW201102108A (en) * | 2009-07-15 | 2011-01-16 | Univ Nat Pingtung Sci & Tech | A natural facial-cleaning component |
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| TW201102108A (en) * | 2009-07-15 | 2011-01-16 | Univ Nat Pingtung Sci & Tech | A natural facial-cleaning component |
Non-Patent Citations (2)
| Title |
|---|
| Mingmei Zhou，et al.Transcription and metabonomic profiling reveal synergistic effects of quercetin and resveratrol supplementation in high fat diet fed mice.《Journal of Proteome Research》.2012,第11卷第4961-4971页. * |
| 单俊杰,等.抗糖尿病及并发症黄酮类化合物的研究进展.《中国新药杂志》.2008,第17卷(第12期),第998-1006页. * |
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