CN103255193B - Ginsenoside conversion method by use of ginseng endophytic Paenibacillus polymyxa - Google Patents
Ginsenoside conversion method by use of ginseng endophytic Paenibacillus polymyxa Download PDFInfo
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Abstract
The invention relates to the field of biotechnology and specifically discloses a ginsenoside conversion method by the use of ginseng endophytic Paenibacillus polymyxa. The endophytic Paenibacillus polymyxa was preserved in the China General Microbiological Culture Collection Center on February, 5th, 2013. The address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The collection number is CGMCC No. 7250. The ginseng endophytic Paenibacillus polymyxa has high conversion activity. By the adoption of the method, contents of ginsenoside monomer Rg1, Re, Rf, Rg2, Rb1, Rb2, Rb3 and Rd are raised are induced. According to the strain, a raw material ginseng powder can be directly used as a medium for conversion of saponins. The method is simple, requires low cost, and is suitable for industrial large-scale production.
Description
Technical field
The present invention relates to biological technical field, be a kind of ginseng endogenetic polymexa bacillus (
paenibacillus polymyxa) transform the method for ginsenoside.
Background technology
Ginsenoside is the effective component of ginseng, but ginseng content is lower, and particularly rare ginsenoside content is lower.In addition after ginsenoside oral administration, almost can not decompose by human intestines and stomach's digestive ferment, but depend in enteron aisle the utilization that just can be absorbed by the body after peculiar microbial metabolism, and play pharmacological action.Panoxadiol saponins can be hydrolyzed β-(1-2)-glucoside bond on β-(1-6)-glycosidic link on C20 and C3 by the enzyme that mainly produced by microorganism of transformation of energy, then Rd is generated, Rd is hydrolyzed further and generates F2 or Rg3, F2 is then hydrolyzed into C-K, Rg3 can be converted into Rh2, finally changes into diol type ginsengenin.Twentieth century Japan's seventies Gang Ji etc., transform ginsenoside with the fermented liquid of soil bacteria and obtain C-K, the nineties, Japan's foot bridge etc. was to having found the metabolic mechanism of ginsenoside Rb1 and ginsenoside Rg1: absorb after enteric microorganism transforms, Rb1 → Rd → F2 → C-K → aglycon; Su Jin-Huan in 2006 etc. isolate bacterial strain from soil
fusarium proliferatumeCU2042, this bacterium can be hydrolyzed Ginsenoside Rg3 becomes Rh2, and transformation efficiency is 60.3%.The utilizations such as Yousef
pytium irregulaebacterium microbe conversion glycols ginsenoside Rb1, Rb2, Rc, Rd etc. are converted into F2.2007, doctor Cheng Leqin screened a strain new bacteria from soil
microbacterium esteraromatiumgS514, it is Rd, Rg3, F2 and C-K that enzymatic production transforms Rb1.Tong Xin etc. have invented and have carried out conversion with moral formula Bacterium lacticum subspecies bulgaricus to Radix Ginseng total saponins and obtain the rare saponin(e Rh2 of ginseng.Yang Yuan in 2011 is superfine screen a strain Fusarium moniliforme (
fuasarium moniliforme) to transform notoginseng stem and leaf total saponin Rb1, Rd be C-K.Can produce rare saponin(e by microbial transformation or improve its output as seen.But have no the report transforming ginsenoside with ginseng endogenetic polymexa bacillus.
Summary of the invention
In view of the problem that ginsenoside monomer content in ginseng is low, a kind of ginseng endogenetic polymexa bacillus is the object of the present invention is to provide to transform the method for ginsenoside, to produce rare saponin(e or to improve its output, and then improve pharmaceutical use and the bioavailability of ginseng.
example 1 one kinds of ginseng endogenetic polymexa bacillus transform the method for ginsenoside
The present invention relates to biological technical field, is a kind of method that ginseng endogenetic polymexa bacillus transforms ginsenoside.Endogenetic polymexa bacillus of the present invention, is kept at China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC 7250.Ginseng endogenetic polymexa bacillus of the present invention has higher activity of conversion.The method increase ginsenoside monomer Rg1, Re, Rf, Rg2, Rb1, Rb2, Rb3, Rd content.This bacterial strain directly can utilize and transform saponin(e raw material ginseng powder is substratum, and method is easy, and cost is low, is conducive to industrialization scale operation.Concrete scheme is as follows:
1.a kind of ginseng endogenetic polymexa bacillus (
paenibacillus polymyxa) transform the method for ginsenoside, be transform ginseng with ginseng endogenetic polymexa bacillus to produce ginsenoside monomer Rg1, Re, Rf, Rg2
,rb1, Rb2, Rb3, Rd method, is characterized in that it is completed by following steps:
(1) ginseng endogenetic polymexa bacillus: be kept at China Committee for Culture Collection of Microorganisms's common micro-organisms center on February 5th, 2013, address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number CGMCC No.7250;
(2) ginseng endogenetic polymexa bacillus transforms the preparation of ginsenoside seed liquor: the activation of ginseng endogenetic polymexa bacillus adopts PDB substratum, and at 25 DEG C, in thermostat container, 120r/min shaking culture is spent the night, and mensuration bacterial concentration is OD
600as seed liquor when=0.5, namely concentration is OD
600the ginseng endogenetic polymexa bacillus seed liquor of=0.5;
(3) ginseng endogenetic polymexa bacillus transforms ginsenoside medium preparing: water 100ml, 80 order ginseng powder 3g, cool and get final product after 121 DEG C of sterilizing 20min.
(4) ginseng endogenetic polymexa bacillus transforms ginsenoside culture condition: add 100ml ginseng endogenetic polymexa bacillus in 250ml triangular flask and transform ginsenoside substratum, the ginseng endogenetic polymexa bacillus inoculating 2% culture volume transforms ginsenoside seed liquor, rotating speed is that 150r/min concussion is cultivated, culture temperature is 25 DEG C, and incubation time is 12d.
2.the method that ginseng endogenetic polymexa bacillus transforms ginsenoside is characterized in that the method is suitable for transforming ginseng with ginseng endogenetic polymexa bacillus and produces ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rb2, Rb3, Rd.
test example 1 different fermentations temperature transforms the impact of ginsenoside to ginseng endogenetic polymexa bacillus
1 materials and methods
1.1 ginseng endogenetic polymexa bacillus actication of culture
Access under 4 degree of inclined-plane ginseng endogenetic polymexa bacillus bacterial classification aseptic techniques of preserving in liquid PDB substratum, at 25 DEG C, in thermostat container, 150r/min shaking culture is spent the night, and measuring its concentration is OD
600as fermentation seed liquid when=0.5.
1.2 ginseng endogenetic polymexa bacillus transform ginsenoside
100ml water and 3g ginseng powder is added respectively in 12 bottles of 250ml triangular flasks, 121 DEG C, 20min sterilizing cools, aseptically, respectively connect with liquid-transfering gun the ginseng endogenetic polymexa bacillus seed liquor that 1ml concentration is OD600=0.5, cultivate 25 DEG C, 30 DEG C, 35 DEG C 150r/min condition concussions respectively, each temperature 4 repetition, after cultivating 8d, obtain ginseng endogenetic polymexa bacillus converted product.
in 1.3 ginseng endogenetic polymexa bacillus converted products, ginseng saponins measures
After the 50-60 DEG C of water-bath of ginseng endogenetic polymexa bacillus converted product is dried, soxhlet extraction extracts saponin(e, with ginseng saponins Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd content in Syrups by HPLC converted product.
2, data processing
Spss17.0 software carries out statistical study to data.
3, results and analysis
Temperature is the important factor affecting bacteria growing, can affect the activity of enzyme, therefore also directly affects saponin content in ginseng endogenetic polymexa bacillus converted product.As shown in table 1, except ginseng saponins Rd, during 25 DEG C of fermentations, ginseng saponins content and 9 kinds of ginseng saponinses add and value reaches maximum.During 30 DEG C of fermentations, ginseng saponins Rd value is the highest, and when ginseng saponins Re, Rf, Rb1, Rb2, Rb3 content and 9 kinds of ginseng saponinses add and be worth and 25 DEG C ferment, difference is not remarkable.
table 1 different fermentations temperature transforms the impact (%) of ginsenoside to ginseng endogenetic polymexa bacillus
| Process | Rg1 | Re | Rf | Rb1 | Rg2 | Rc | Rb2 | Rb3 | Rd | Rg1＋Re＋Rf＋Rb1＋Rg2＋Rc＋Rb2＋Rb3＋Rd |
| 25℃ | 0.59 a | 0.34 a | 0.13 a | 0.57 a | 0.04 a | 0.16 a | 0.23 a | 0.04 a | 0.53 b | 2.61 a |
| 30℃ | 0.44 b | 0.33 a | 0.14 a | 0.58 a | 0.04 b | 0.12 b | 0.20 a | 0.04 a | 0.65 a | 2.53 a |
| 35℃ | 0.31 c | 0.21 b | 0.09 b | 0.38 b | 0.03 c | 0.05 c | 0.21 a | 0.01 b | 0.37 c | 1.65 b |
Note: same column different rows Superscript letters identical table differential is different not significantly (P>0.05), and same column different rows Superscript letters difference represents significant difference (P<0.05).
the test example 2 different fermentations time transforms the impact of ginsenoside to ginseng endogenetic polymexa bacillus
1 materials and methods
1.1 ginseng endogenetic polymexa bacillus actication of culture
With test example 1.
1.2 ginseng endogenetic polymexa bacillus transform ginsenoside
100ml water and 3g ginseng powder is added respectively in 24 bottles of 250ml triangular flasks, 121 DEG C, 20min sterilizing cools, aseptically, the ginseng endogenetic polymexa bacillus seed liquor that 1ml concentration is OD600=0.5 is respectively connect, at 25 DEG C with liquid-transfering gun, 150r/min shakes cultivation, after cultivating 1d, 2 d, 4 d, 8 d, 12 d, 16d respectively, obtain ginseng endogenetic polymexa bacillus converted product, repetition of each time 4.
in 1.3 ginseng endogenetic polymexa bacillus converted products, ginseng saponins measures
With test example 1.
2, data processing
With test example 1.
3, results and analysis
Microorganism has respective growth cycle, and the length of fermentation time not only affects microbial growth state, also affects the conversion of microorganism to ginsenoside, in table 2.Except ginseng saponins Rc and Rb2, add with ginseng saponins content and 9 kinds of ginseng saponinses in the prolongation ginseng endogenetic polymexa bacillus converted product of fermentation time and be worth and all improve, ginseng saponins Rg1, Re, Rg2, Rc, Rb3 content and 9 kinds of ginseng saponinses add and be worth 12 days and fermentation in 16 days time difference not remarkable.From fermentation period and 9 kinds of saponin content angle analysis ferment 12 days time effect better.
the table 2 different fermentations time transforms the impact of ginsenoside to ginseng endogenetic polymexa bacillus(%)
| Process (my god) | Rg1 | Re | Rf | Rb1 | Rg2 | Rc | Rb2 | Rb3 | Rd | Rg1＋Re＋Rf＋Rb1＋Rg2＋Rc＋Rb2＋Rb3＋Rd |
| 1 | 0.31 c | 0.17 e | 0.08 f | 0.31 e | 0.02 c | 0.22 b | 0.15 b | 0.03 d | 0.19 f | 1.47 e |
| 2 | 0.48 b | 0.28 d | 0.10 e | 0.45 d | 0.03 b | 0.26 a | 0.22 a | 0.04 c | 0.31 e | 2.16 d |
| 4 | 0.54 a | 0.31 c | 0.11 d | 0.51 cd | 0.03 b | 0.26 a | 0.22 a | 0.04 c | 0.37 d | 2.38 c |
| 8 | 0.59 a | 0.34 b | 0.13 c | 0.56 c | 0.04 a | 0.16 c | 0.23 a | 0.04 b | 0.53 c | 2.61 b |
| 12 | 0.56 a | 0.40 a | 0.16 b | 0.69 b | 0.04 a | 0.15 c | 0.11 c | 0.05 | 0.86 b | 3.02 a |
| 16 | 0.59 a | 0.41 a | 0.17 a | 0.77 a | 0.05 a | 0.16 c | 0.08 d | 0.05 a | 0.94 a | 3.22 a |
Note: same column different rows Superscript letters identical table differential is different not significantly (P>0.05), and same column different rows Superscript letters difference represents significant difference (P<0.05).
test example 3 difference connects bacterium amount transforms ginsenoside impact on ginseng endogenetic polymexa bacillus
1 materials and methods
1.1 ginseng endogenetic polymexa bacillus actication of culture
With test example 1.
1.2 ginseng endogenetic polymexa bacillus transform ginsenoside
100ml water and 3g ginseng powder is added respectively in 16 bottles of 250ml triangular flasks, 121 DEG C, 20min sterilizing cools, aseptically, by raw bacillus polymyxa seed liquor in the ginseng that liquid-transfering gun respectively meets 0.5 ml, 1ml, 2 ml, 4 ml concentration are OD600=0.5, each inoculum size 4 repetition, at 25 DEG C, 150r/min shakes cultivation, after cultivating 12 d, obtains raw bacillus polymyxa converted product in ginseng.
in 1.3 ginseng endogenetic polymexa bacillus converted products, ginseng saponins measures
With test example 1.
2, data processing
With test example 1.
3, results and analysis
Connecing bacterium amount in fermenting process is also the important factor affecting microorganism growth, and it is as shown in table 3 that difference connects the impact of bacterium amount on ginseng endogenetic polymexa bacillus conversion ginsenoside.With 9 kinds of ginseng saponins content in the increase ginseng endogenetic polymexa bacillus converted product of inoculum size and and add and be worth and all improve, but 2ml and 4ml process group difference is not remarkable.Effect when bacterium amount is 2ml is connect better from fermentation costs and 9 kinds of saponin content angle analysis.
table 3 difference connects bacterium amount transforms ginsenoside impact (%) on ginseng endogenetic polymexa bacillus
| Process | Rg1 | Re | Rf | Rb1 | Rg2 | Rc | Rb2 | Rb3 | Rd | Rg1＋Re＋Rf＋Rb1＋Rg2＋Rc＋Rb2＋Rb3＋Rd |
| 0.5ml | 0.50 c | 0.39 a | 0.17 a | 0.71 a | 0.04 c | 0.16 a | 0.30 a | 0.06 a | 0.66 a | 3.00 b |
| 1ml | 0.59 bc | 0.40 a | 0.16 a | 0.74 a | 0.04 bc | 0.16 a | 0.30 a | 0.06 a | 0.67 a | 3.12 ab |
| 2ml | 0.77 a | 0.41 a | 0.16 a | 0.77 a | 0.05 ab | 0.17 a | 0.29 a | 0.07 a | 0.68 a | 3.36 a |
| 4ml | 0.75 ab | 0.41 a | 0.15 a | 0.77 a | 0.05 a | 0.15 a | 0.30 a | 0.08 a | 0.72 a | 3.38 a |
Note: same column different rows Superscript letters identical table differential is different not significantly (P>0.05), and same column different rows Superscript letters difference represents significant difference (P<0.05).
Claims (1)
1. a ginseng endogenetic polymexa bacillus (
paenibacillus polymyxa) transform the method for ginsenoside, be transform ginseng with ginseng endogenetic polymexa bacillus to produce ginsenoside monomer Rg1, Re, Rf, Rg2
,rb1, Rb2, Rb3, Rd method, is characterized in that it is completed by following steps:
(1) ginseng endogenetic polymexa bacillus: be kept at China Committee for Culture Collection of Microorganisms's common micro-organisms center on February 5th, 2013, address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number CGMCC No.7250;
(2) ginseng endogenetic polymexa bacillus transforms the preparation of ginsenoside seed liquor: the activation of ginseng endogenetic polymexa bacillus adopts PDB substratum, and at 25 DEG C, in thermostat container, 120r/min shaking culture is spent the night, and mensuration bacterial concentration is OD
600as seed liquor when=0.5, namely concentration is OD
600the ginseng endogenetic polymexa bacillus seed liquor of=0.5;
(3) ginseng endogenetic polymexa bacillus transforms ginsenoside medium preparing: water 100ml, and 80 order ginseng powder 3g, cool and get final product after 121 DEG C of sterilizing 20min;
(4) ginseng endogenetic polymexa bacillus transforms ginsenoside culture condition: add 100ml ginseng endogenetic polymexa bacillus in 250ml triangular flask and transform ginsenoside substratum, the ginseng endogenetic polymexa bacillus inoculating 2% culture volume transforms ginsenoside seed liquor, rotating speed is that 150r/min concussion is cultivated, culture temperature is 25 DEG C, and incubation time is 12d.
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| KR101423100B1 (en) * | 2014-01-17 | 2014-07-25 | 백제홍삼 주식회사 | Fabrication method of enhancing ginsenoside Rg3 and Rb1 of red ginseng |
| CN106367371B (en) * | 2016-09-18 | 2019-04-02 | 中南民族大学 | The enterobacter cloacae clx-14 bacterial strain and application thereof of one plant of production chonglou saponin I |
| CN107312720B (en) * | 2017-03-01 | 2020-02-04 | 东北林业大学 | Cochinchinensis endophytic fungus for efficiently converting ginsenoside Rb1 into Rd and application thereof |
| CN107663528A (en) * | 2017-04-10 | 2018-02-06 | 吉林农业大学 | A kind of Cu2+The method that ion promotes Paenibacillus polymyxa conversion ginsenoside |
| CN106929465A (en) * | 2017-04-10 | 2017-07-07 | 吉林农业大学 | A kind of Cu2+Ion promotes Paenibacillus polymyxa propagation and its application process |
| CN108034614B (en) * | 2018-01-19 | 2022-05-27 | 吉林农业大学 | Method for simultaneously degrading 5 pesticide residues by using Paenibacillus polymyxa |
| CN108753900A (en) * | 2018-06-13 | 2018-11-06 | 延边大学 | A kind of method that the fermentation of ginseng endophyte prepares rare ginsenoside |
| CN111979139B (en) * | 2020-05-30 | 2022-04-01 | 郑州大学 | Bacillus belgii G9y and application thereof in ginsenoside biotransformation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982438A (en) * | 2006-05-24 | 2007-06-20 | 辽宁新中现代医药有限公司 | Bacillus and production of monodesmosidic panasaponin and aglucon therewith |
| CN102657331A (en) * | 2012-05-02 | 2012-09-12 | 金光洙 | Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts |
| KR20120122990A (en) * | 2011-04-29 | 2012-11-07 | 한국생명공학연구원 | Glycoside hydrolase From lactic acid bacteria And Use Thereof |
-
2013
- 2013-03-05 CN CN201310067689.5A patent/CN103255193B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982438A (en) * | 2006-05-24 | 2007-06-20 | 辽宁新中现代医药有限公司 | Bacillus and production of monodesmosidic panasaponin and aglucon therewith |
| KR20120122990A (en) * | 2011-04-29 | 2012-11-07 | 한국생명공학연구원 | Glycoside hydrolase From lactic acid bacteria And Use Thereof |
| CN102657331A (en) * | 2012-05-02 | 2012-09-12 | 金光洙 | Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts |
Non-Patent Citations (2)
| Title |
|---|
| Biotransformation of ginsenoside Rb1 to ginsenoside Rd by highly substrate-tolerant Paecilomyces bainier 229-7;Li Ye et al;《Bioresource Technology》;20100601;第101卷;7872-7876 * |
| 海洋枯草芽孢杆菌Bs-1 产生多种抗真菌活性物质;谢海平等;《中山大学学报(自然科学版)》;20030531;第42卷(第3期);122-123 * |
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