CN103245708A - Magnet-based capillary electrophoresis electrochemical detection electrode locating device - Google Patents
Magnet-based capillary electrophoresis electrochemical detection electrode locating device Download PDFInfo
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Abstract
The invention discloses a magnet-based capillary electrophoresis electrochemical detection electrode locating device and belongs to the technical field of capillary electrophoresis. The device comprises an electrode bracket containing a magnet, a detection electrode, a capillary, a detection pool, a capillary locating pipe, an electrode locating pipe, a screw rod and a micrometer head, wherein the micrometer head is fixed on a bottom plate through an installing sleeve, the outer diameter of the screw rod is the same as the diameter of a circular hole in the electrode bracket, and the top end of the screw rod is attached to the circular hole through the magnet and can rotate freely; the capillary locating pipe and the electrode locating pipe are respectively installed on two side walls of the detection pool and are concentrically aligned, the capillary is fixed in the locating pipe, the detection electrode is installed in the small hole of the electrode bracket and is aligned with the capillary after being inserted into the locating pipe, the electrode bracket drives the detection electrode to move back and forth by rotating a knob of the micrometer head so as to realize the precise adjustment on the space between an outlet of the capillary and the detection electrode.
Description
Technical field
The invention belongs to the capillary electrophoresis technique field, be specifically related to a kind of capillary electrophoresis electrochemical detecting electrode locating device based on magnet.
Background technology
Capillary Electrophoresis be a kind of be driving force with the high-voltage electric field, be split tunnel with the kapillary, according to the difference in mobility and the distribution behavior and realize the efficient liquid phase separation method of compartment analysis between each component in the sample.Since Joegenson in 1981 and Luckas propose Capillary Electrophoresis first [1], this technology is efficient with it, fast, degree of separation is high, highly sensitive, reagent dosage is few, low consumption and integrated level advantages of higher have caused domestic and international analysis and life science circle relevant expert's extensive concern, obtained to use widely in fields such as biomedical research, food and medicine analysis, clinical diagnosis, environmental monitorings [2,3].
Capillary Electrophoresis instrument system mainly comprises separation capillary, high-voltage DC power supply, buffer solution pond, detecting device, data recording and processing unit etc., and the detection technique that Capillary Electrophoresis is commonly used has uv absorption detection method, laser-Induced Fluorescence Detection method, Mass Spectrometer Method method and electrochemical assay etc. at present.Wherein Electrochemical Detection have that highly sensitive, little power consumption, dead volume are little, the small and exquisite advantage such as easy of integration of electrode and control instrument, be the desirable detection technique of Capillary Electrophoresis, obtained attention more and more widely in recent years.
Electrochemical Detection is commonly used to detect [4] and [5] two kinds of detecting patterns of styletable detection from post, for detecting from column electrochemical, isolates separation voltage with the porous contact between separation capillary and detection kapillary; For newel post's Electrochemical Detection, little dish detecting electrode is positioned at the capillary outlet place.Detect for capillary electrophoresis electrochemical, the accurate location between separation capillary and detecting electrode is the principal element [3] of its widespread use of restriction.Usually adopt three-dimensional adjustable narishige that detecting electrode is alignd with capillary outlet, owing to the poor reproducibility of each manual adjustment, influence accuracy and the precision of capillary electrophoresis analysis.Three-dimensional adjustable narishige volume is bigger, and integrated level is not high, and anti-seismic performance is not good, and positioning action complexity, time-consuming needs professional operating personnel [3].So, develop the easy detecting electrode of Capillary Electrophoresis cheaply locating device, the step that simplifies the operation improves the location reappearance of detecting electrode and precision to the development of capillary electrophoresis electrochemical detection technique and uses significant.
The present invention utilizes the kapillary positioning pipe of concentric collimation and detecting electrode positioning pipe to make kapillary and detecting electrode alignment, by the electrode suppor that includes magnet and micrometer head, knob by the rotation micrometer head moves forward and backward electrode suppor drive detecting electrode, realizes the minute adjustment of distance between capillary outlet and detecting electrode top.The present invention has that processing is easy, cost is low, high integration, easy and simple to handle and bearing accuracy advantages of higher, and can with technology couplings such as micro-fluidic chip, capillary electric chromatogram, flow injection, in fields such as environmental monitoring, clinical diagnosis, food and medicine analyses good prospects for application is arranged.
List of references
[1] Jorgenson, J.W., Lukacs, K.D., Anal. Chem. 1981, 53, 1298–1302.
[2] Yu, H., Xu, X.Y., Sun, J.Y., You, T.Y., Cent. Eur. J. Chem. 2012, 10, 639-651.
[3] Chen, G., Zhu, Y.Z., Wang, Y.F., Xu, X.J., Lu, T., Current Med. Chem. 2006, 13, 2467–2485.
[4] O’Shea, T.J., Lunte, S.M., Anal. Chem. 1993, 65, 948–951.
[5] Chen, Q.W., Zhang, L.Y., Chen, G., Anal. Chem. 2012, 84, 171–178.。
Summary of the invention
The objective of the invention is to propose that a kind of making is simple, cheap, integrated level is high, and the capillary electrophoresis electrochemical detecting electrode locating device based on magnet easy and simple to handle, favorable reproducibility.
The capillary electrophoresis electrochemical detecting electrode locating device based on magnet that the present invention proposes, its structure is made up of the electrode suppor 14 that includes magnet 3, detecting electrode 8, kapillary 9, kapillary positioning pipe 11, detecting electrode positioning pipe 13, detection cell 12, micrometer head 17, steel screw rod 15, mounting sleeve 16 and base plate 22 as shown in Figure 1; Magnet 3 is installed on one side and has in the electrode suppor 14 of circular hole 4; Mounting sleeve 16 is installed on the base plate 22, and micrometer head 17 is fixing, and the external diameter of steel screw rod 15 is identical with the diameter of circular hole 4, and the magnetic force by magnet 3 is attached in the circular hole 4 top of steel screw rod 15 and also can rotates freely; Be provided with the mylar 5 of reducing friction of being used for of one deck 25-100 micron thickness at micrometer head 17 and 3 of magnet; Kapillary positioning pipe 11 and electrode positioning pipe 13 are installed in respectively on the relative two side of detection cell 12, and both collimate with one heart, kapillary 9 is fixed in the kapillary positioning pipe 11 by silicone rubber plate 10, detecting electrode 8 is fixed in the aperture 6 on the electrode suppor 14 by two silicone rubber plates 7, and by electrode positioning pipe 13 and kapillary 9 outlet collimations; Making the cylindrical steel screw rod 15 of suction on magnet 3 drive the electrode suppor 14 that detecting electrode 8 is installed by the knob of regulating micrometer head 17 moves forward and backward, regulates with the high precision that realizes distance between kapillary 11 outlets and detecting electrode 8 tops.
Among the present invention, the magnet 3 that is used for making detecting electrode locating device can be ndfeb magnet, ferrite lattice or magnet steel etc., is the rectangular parallelepiped piece, is of a size of 15-25 mm * 8-15 mm * 1-3 mm.Size according to magnet 3, poly (methyl methacrylate) plate or polystyrene board that 0.5-2 mm is thick cut into small pieces by numerical control laser cutter, by the bonding electrode suppor 14 that gets of organic solvents such as chloroform, its external dimensions is 30-40 mm * 17-30 mm * 4-6 mm, interior space dimension is 25-35 mm * 15-25 mm * 1-3 mm, the length of inner space length and width and magnet 3 and consistency of thickness, the position of determining magnet 3 with organic glass sheet or polystyrene sheet is filled in electrode suppor 14 bottoms.
Among the present invention, two apertures 6 that the top of electrode suppor 14 is used for the installation and measuring electrode are square hole, it is of a size of: 1.5-3.5 mm * 4-6 mm, by two silicone rubber plate 7(1.5-3.5 mm * 1.5-2.5 mm * 6-8 mm), detecting electrode is installed on the support 14; Circular hole 4 diameters at the one side middle part of electrode suppor 14 are 6 mm, and circular hole centre distance base plate 22 bottoms are 10-20 mm.
Among the present invention, micrometer head 17 has commercially available, precision is 0.01 mm, the stroke of its screw rod is 0-25 mm, by a columnar organic glass sheet or polystyrene sleeve pipe 16(internal diameter 13 mm, external diameter 18 mm, long 18 mm), be fixed on the base plate 22, there are two organic glass or polystyrene pad 18(3 mm * 3 mm * 18 mm that are bonded on the base plate 22 tubular shell 16 both sides), increase bond strength and the stability of tubular shell 16 on base plate.Base plate 22 is transparent organic glass or polystyrene board (15-25 mm * 40-60 mm * 3-7 mm).Detection cell 12 and support employing thickness thereof are that transparent organic glass sheet or the polystyrene sheet of 1-3 mm is bonded, the inside dimension of detection cell 12 is 15-30 mm * 10-20 mm * 10-20 mm, by machine drilling and bonding technology with kapillary positioning pipe 11, detecting electrode positioning pipe 13, contrast electrode 19, auxiliary electrode 20, ground-electrode 21 is installed in the detection cell 12, kapillary positioning pipe 11, detecting electrode positioning pipe 13 is positioned at the upper area of detection cell 12, contrast electrode 19, auxiliary electrode 20, ground-electrode 21 is positioned at the central region of detection cell 12, and its position is referring to shown in Figure 1.
Among the present invention, the internal diameter 0.35-0.6 mm of kapillary positioning pipe 11, external diameter 0.6-1 mm, long 10-20 mm, the internal diameter 0.35-0.6 mm of detecting electrode positioning pipe 13, external diameter 0.6-1 mm, long 10-20 mm, the internal diameter of kapillary positioning pipe 11 and detecting electrode positioning pipe 13 than insert wherein kapillary 9 and the external diameter of detecting electrode 8 exceed 20-50 μ m, all adopt stainless steel capillary to make, before the installation, earlier kapillary (internal diameter 10-320 μ m, external diameter 320-450 μ m) is inserted two pipes, then kapillary positioning pipe 11 and detecting electrode positioning pipe 13 are inserted respectively detection cell 12 in the hole of right two side, bonding with transparent epoxy resin, two positioning pipes are collimated with one heart.Wherein auxiliary electrode 20 and ground-electrode 21 all use platinum filament (diameter 100-500 μ m), and contrast electrode 19 is silver/silver chloride electrode.
Among the present invention, the method for making of detecting electrode is: as shown in Figure 2, with copper wire 24(diameter 100-200 μ m, long 10-20 cm) one is inserted a fused quartz kapillary 23(internal diameter 50-320 μ m, external diameter 320-450, long 3-6 cm) in, the end of copper wire 24 in pipe is from about 4 mm of capillary end, and kapillary 23 other ends are fixed by hot melt adhesive 25 and copper wire.Epoxy resin and hardening agent are mixed well the back to be mixed by mass ratio 1:1 with conductive material such as dag and mixes well, capillary end is vertically inserted this potpourri, until potpourri with the pipe in copper wire 24 contact, heating is after 40-80 minute in 50-70 ℃ of baking oven, end electrodes packing material 26 usefulness abrasive paper for metallograph are worn into disk and are got electrode end surface, namely make detecting electrode 21.Whole parts are in place according to accompanying drawing 1 namely get capillary electrophoresis electrochemical detecting electrode locating device based on magnet.
Localization method based on capillary electrophoresis electrochemical detecting electrode 8 of the present invention: kapillary 9 is inserted in the kapillary positioning pipe 11, and in detection cell, expose about 1-3 mm, make kapillary 9 fixing by the outer silicon rubber stator 10 of detection cell, then, regulating micrometer head 17 makes detecting electrode 8 insert electrode positioning pipe 13, contact with kapillary 9 outlets until its top, rotate the knob of micrometer head 17 then, make steel screw rod 15 drive detecting electrode 8 retraction 10-100 μ m, realize the hi-Fix based on the capillary electrophoresis electrochemical detecting electrode.
The present invention is simple in structure, processing is easy, cost is low, integrated level is high, easy and simple to handle, bearing accuracy and location reappearance height, and the collimation location that kapillary 9 and detecting electrode are 8 need not to use the Three dimensional steerable instrument of complex structure and poor stability.Adopted integrated design among the present invention, detection cell 12 and detecting electrode support 14 all are fixed on the base plate 14, the vibrations of base and the movement of position can not cause capillary outlet behind the aligning and the change of 8 relative positions of detecting electrode, have strengthened stability and the reappearance of total system.The present invention has good prospects for application in fields such as environmental monitoring, bio-medical analysis, food analyses.
Description of drawings
Fig. 1 is design diagram of the present invention.Wherein, (A-C) for containing front elevation (A), side view (B) and the oblique view (C) of electrode suppor 14 of magnet 3; (D) for being sandwiched in the detecting electrode 8 of 7 of two silicone rubber plates; (E) be the oblique view that the electrode suppor 14 of detecting electrode 8 is installed; (F) be capillary electrophoresis electrochemical detecting electrode locating device oblique view based on magnet 3 and micrometer head 17.
Fig. 2 is the manufacturing process synoptic diagram of detecting electrode among the present invention.
Fig. 3 detects the Capillary Electrophoresis collection of illustrative plates of the standard mixed solution of p-phenylenediamine (PPD) (a), meta-aminophenol (b) and resorcinol (c) (concentration be 0.5 mM/liter) for using the present invention.
Fig. 4 detects the peak current that obtains and the relation curve of locating number of times for the capillary electrophoresis electrochemical that the present invention is used for p-phenylenediamine (PPD) (a), meta-aminophenol (b) and resorcinol (c) (concentration be 0.5 mM/liter) standard mixed solution.
Fig. 5 detects in the hair dye Capillary Electrophoresis collection of illustrative plates to p-phenylenediamine (PPD) (a), meta-aminophenol (b) and resorcinol (c) for using the present invention.
Number in the figure: 1 is the detecting electrode fixed orifice, and 2 is the organic glass support, and 3 is magnet, 4 is positioning round orifice, and 5 is mylar, and 6 is the detecting electrode mounting hole, 7 use silicone rubber plate for electrode is fixing, 8 is detecting electrode, and 9 is kapillary, and 10 use silicone rubber plate for kapillary is fixing, 11 is the kapillary positioning pipe, 12 is detection cell, and 13 is the detecting electrode positioning pipe, and 14 for containing the detecting electrode support of magnet, 15 is the steel screw rod, 16 is the organic glass sleeve pipe, and 17 is the organic glass pad, and 18 is the detecting electrode guiding tube, 19 is silver/silver chloride contrast electrode, 20 is auxiliary electrode, and 21 is ground-electrode, and 22 is plexiglass base plate, 23 is quartz capillary, 24 is copper wire, and 25 is hot melt adhesive, and 26 is the electrode packing material.
Embodiment
Further describe the present invention below by embodiment and accompanying drawing:
(1) contains the processing of the electrode suppor 14 of magnet
The magnet 3 that is used for making detecting electrode locating device can be ndfeb magnet, is of a size of 20 mm * 10 mm * 2 mm.Size according to magnet 3, the poly (methyl methacrylate) plate that 1 mm is thick cuts into small pieces by numerical control laser cutter, by the bonding electrode suppor 14 that gets of organic solvents such as chloroform, its external dimensions is 32 mm * 24 mm * 4 mm, interior space dimension is 25 mm * 20 mm * 2 mm, require the length of inner space length and width and magnet 3 and thickness always, the base that filling two thick organic glass sheets of 1 mm in electrode suppor bottom are determined magnet 3 is 7 mm from the height of base plate.The top of electrode suppor 14 has processed two square holes (2 mm * 4 mm), by two silicone rubber plate 7(2 mm * 2 mm * 7 mm), detecting electrode 8 is installed on the support 14.Circular hole 4 that diameter is 6 mm of one side middle part processing of support 14, about 12 mm in circular hole centre distance base plate 22 bottoms.
(2) making of detecting electrode 8
As shown in Figure 2, with copper wire 24(diameter 150 μ m, long 15 cm) one is inserted fused quartz kapillary 23(internal diameter 250 μ m, external diameter 370, long 4 cm) in, the end of copper wire 24 in pipe is from about 4 mm of capillary end, and the kapillary other end is fixing with copper wire 24 with hot melt adhesive 25.Epoxy resin and hardening agent are mixed well the back to be mixed by mass ratio 1:1 with conductive material such as dag and mixes well, capillary end is vertically inserted this potpourri, until potpourri with the pipe in copper wire contact, heating is after 60 minutes in 60 ℃ of baking ovens, end electrodes packing material 26 usefulness abrasive paper for metallograph are worn into disk with electrode tip, namely make detecting electrode 21.
(3) based on processing and the assembling of the capillary electrophoresis electrochemical detecting electrode locating device of magnet
As shown in Figure 1, the present invention is made up of the electrode suppor 14 that includes magnet 3, silicone rubber plate 7, detecting electrode 8, kapillary 9, kapillary positioning pipe 11, detecting electrode positioning pipe 13, detection cell 12, micrometer head 17, installation sleeve 16 and base plate 22 etc., and magnet 3 is loaded on one side and has in the electrode suppor 14 of circular hole 4.Can micrometer head 17 is fixing by the installation sleeve 16 that is fixed on the base plate 22, the external diameter of steel screw rod 15 is identical with the diameter of circular hole 4, magnetic force by magnet 3 makes the top of screw rod 15 be attached in the circular hole 4 and can rotate freely, and the mylar 5 of one deck 50 micron thickness is arranged to reduce friction at micrometer head 17 and 3 of magnet.Kapillary positioning pipe 11 and electrode positioning pipe 13 are installed in respectively on the relative two side of detection cell 12, require collimation with one heart, kapillary 9 is fixed in the kapillary positioning pipe 11 by silicon rubber stator 10, detecting electrode 8 is fixed in the aperture on the electrode suppor 14 by two silicone rubber plates 7, and by electrode positioning pipe 13 and kapillary 9 outlet collimations.Make the cylindrical steel screw rod 15 of suction on magnet 3 drive the electrode suppor 14 that detecting electrode 8 is installed by the knob of regulating micrometer head 17 and move forward and backward, thereby the high precision that realizes distance between kapillary 11 outlets and detecting electrode 8 tops is regulated.
Commercially available micrometer head 17 precision are 0.01 mm among the present invention, the stroke of its screw rod is 0-25 mm, by columnar organic glass sheet sleeve pipe 16(internal diameter 13 mm, external diameter 18 mm, long 18 mm), be fixed on the base plate 22, there are organic glass pad 18(3 mm * 3 mm * 18 mm of company that are bonded on the base plate 22 tubular shell 16 both sides), increase bond strength and the stability of tubular shell 16 on base plate.Base plate 22 is transparent poly (methyl methacrylate) plate (20 mm * 50 mm * 5 mm).Detection cell 12 and support employing thickness thereof are that the transparent organic glass sheet of 2 mm is bonded, the inside dimension of detection cell 12 is 20 mm * 15 mm * 15 mm, by machine drilling and bonding technology kapillary positioning pipe 11, detecting electrode positioning pipe 13, contrast electrode 19, auxiliary electrode 20, ground-electrode 21 is installed in the relevant position shown in accompanying drawing 1.Kapillary positioning pipe 11(internal diameter 0.4 mm, external diameter 0.7 mm, long 15 mm) and detecting electrode positioning pipe 13(internal diameter 0.4 mm, external diameter 0.7 mm, long 15 mm) internal diameter all adopts stainless steel capillary than insertion kapillary 9 wherein and the high about 30 μ m of external diameter of detecting electrode 8.Before the installation, earlier with kapillary (internal diameter 25 μ m, external diameter 370 μ m) insert two pipes, then kapillary positioning pipe 11 and detecting electrode positioning pipe 13 are inserted respectively detection cell 12 in the hole of right two side, bonding with transparent epoxy resin, two positioning pipes are collimated with one heart.Wherein auxiliary electrode 20 and ground-electrode 21 are platinum filament (diameter 250 μ m), and contrast electrode 19 is silver/silver chloride electrode.
(4) based on operation and the application thereof of the capillary electrophoresis electrochemical detecting electrode locating device of magnet
In order to test performance of the present invention, itself and high-voltage DC power supply and electrochemical detector are assembled into the capillary electrophoresis electrochemical detection system, be used for 0.5 mM/liter p-phenylenediamine (PPD) (a), meta-aminophenol (b) and resorcinol (c) standard mixed solution the capillary electrophoresis separation analysis, obtain electrophoresis pattern and see accompanying drawing 3, three components can be separated in 11 minutes fully, and the result is satisfactory.Test condition is: separation and sample introduction voltage are 9 kV, and sample injection time is 5 s, and buffer solution is that (pH 9 for 50 mM borate buffer solutions.2), detecting electrode is diameter 250 μ m graphite-epoxy resin composite material disk electrodes, and detecting current potential is+0.8 V (with respect to silver/silver chloride contrast electrode).
Accompanying drawing 4 for use the present invention detect 0.5 mM/liter the peak current of p-phenylenediamine (PPD), meta-aminophenol and resorcinol standard mixed solution and the relation curve of location number of times, the relative standard deviation of locating the p-phenylenediamine (PPD), meta-aminophenol and the resorcinol peak current that record for 15 times is respectively 4.2 %, 4.4% and 3. 5%, shows that location of the present invention reappearance is good.
For further verifying practicality of the present invention, development also be used to the detection (accompanying drawing 5) of p-phenylenediamine (PPD) in the hair dye, meta-aminophenol and resorcinol based on the capillary electrophoresis electrochemical detecting electrode locating device of magnet, the content that records p-phenylenediamine (PPD), meta-aminophenol and resorcinol in certain brand hair dye is respectively 24.49,2.836 with 1.741 mg/g, meet national standard.
Claims (7)
1. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet is characterized in that being made up of the electrode suppor that includes magnet (3) (14), detecting electrode (8), kapillary (9), kapillary positioning pipe (11), detecting electrode positioning pipe (13), detection cell (12), mounting sleeve (16), micrometer head (17), steel screw rod (15), mounting sleeve (16) and base plate (22); Magnet (3) is installed on one side and has in the electrode suppor (14) of circular hole (4); Mounting sleeve (16) is installed on the base plate (22), and it is micrometer head (17) is fixing, the external diameter of steel screw rod (15) is identical with the diameter of circular hole (4), and the magnetic force by magnet (3) makes the top of steel screw rod (15) be attached in the circular hole (4) and can rotate freely; Between micrometer head (17) and magnet (3), be provided with the mylar (5) reduce friction of being used for of one deck 25-100 micron thickness; Kapillary positioning pipe (11) is installed in respectively on the relative two side of detection cell (12) with electrode positioning pipe (13), and both collimate with one heart, kapillary (9) is fixed in the kapillary positioning pipe (11) by silicone rubber plate (10), (8 are fixed on electrode suppor by two silicone rubber plates 7 (in the aperture on 14 (6), and passes through electrode positioning pipe (13) and kapillary (9) outlet collimation to detecting electrode; Making the cylindrical steel screw rod (15) of suction on magnet (3) drive the electrode suppor (14) that detecting electrode (8) are installed by the knob of regulating micrometer head (17) moves forward and backward, regulates with the high precision that realizes distance between kapillary (11) outlet and detecting electrode (8) top.
2. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 1, it is characterized in that described kapillary (9) is inserted in the kapillary positioning pipe (11), in detection cell (12), expose the 1-3 millimeter, regulating micrometer head (17) makes the top of detecting electrode (8) contact with kapillary (9) outlet, rotate the knob of micrometer head (17) then, make steel screw rod (15) drive detecting electrode (8) retraction 10-100 micron, realize the hi-Fix of detecting electrode.
3. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 1, it is characterized in that described magnet (3) is ndfeb magnet, ferrite lattice or magnet steel, be the rectangular parallelepiped piece, be of a size of 15-25 mm * 8-15 mm * 1-3 mm.
4. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 3, it is characterized in that described electrode suppor (14), its external dimensions is 30-40 mm * 17-30 mm * 4-6 mm, interior space dimension is 25-35 mm * 15-25 mm * 1-3 mm, length and the consistency of thickness of inner space length and width and magnet (3), the position of determining magnet (3) with organic glass sheet or polystyrene sheet is filled in electrode suppor (14) bottom.
5. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 3, it is characterized in that described electrode suppor (14) top is square hole for two apertures (6) of installation and measuring electrode, it is of a size of: 1.5-3.5 mm * 4-6 mm; Circular hole (4) diameter at the one side middle part of electrode suppor (14) is 6 mm, and circular hole centre distance base plate (22) bottom is 10-20 mm.
6. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 3, the inside dimension that it is characterized in that described detection cell (12) is 15-30 mm * 10-20 mm * 10-20 mm.
7. the capillary electrophoresis electrochemical detecting electrode locating device based on magnet according to claim 6, the internal diameter that it is characterized in that described kapillary positioning pipe (11) is 0.35-0.6 mm, external diameter is 0.6-1 mm, long is 10-20 mm, the internal diameter of detecting electrode positioning pipe (13) is 0.35-0.6 mm, external diameter is 0.6-1 mm, and long is 10-20 mm; The internal diameter of kapillary positioning pipe (11), detecting electrode positioning pipe (13) than insert wherein kapillary (9) and the external diameter of detecting electrode (8) exceed 20-50 μ m; The internal diameter of kapillary (9) is 10-320 μ m, and external diameter is 320-450 μ m.
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| CN108362756A (en) * | 2018-01-05 | 2018-08-03 | 复旦大学 | Self-positioning capillary electrophoretic ampere detecting tank of a kind of magnetic force assembling and preparation method thereof |
| CN108362756B (en) * | 2018-01-05 | 2020-07-28 | 复旦大学 | Magnetic assembly self-positioning capillary electrophoresis ampere detection tank and preparation method thereof |
| CN110296932A (en) * | 2019-07-25 | 2019-10-01 | 佛山科学技术学院 | A kind of electro-chemical test sample and preparation method thereof |
| CN116124861A (en) * | 2022-12-23 | 2023-05-16 | 复旦大学 | High-concentricity capillary electrophoresis electrochemical detection electrode and preparation method of fused ball thereof |
| CN116124861B (en) * | 2022-12-23 | 2024-09-06 | 复旦大学 | High-concentricity capillary electrophoresis electrochemical detection electrode and preparation method of fused ball thereof |
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