CN103243484A - Electrostatic spinning preparation method for hybrid nanofiber membrane containing P(LLA-CL) and magnesium metal - Google Patents
Electrostatic spinning preparation method for hybrid nanofiber membrane containing P(LLA-CL) and magnesium metal Download PDFInfo
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- CN103243484A CN103243484A CN2013101785194A CN201310178519A CN103243484A CN 103243484 A CN103243484 A CN 103243484A CN 2013101785194 A CN2013101785194 A CN 2013101785194A CN 201310178519 A CN201310178519 A CN 201310178519A CN 103243484 A CN103243484 A CN 103243484A
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Abstract
The invention relates to an electrostatic spinning preparation method for a hybrid nanofiber membrane containing P(LLA-CL) and magnesium metal. The method comprises the steps of adding magnesium powder into a lactate caprolactone copolymer P(LLA-CL) solution, dissolving to obtain an electro-spinning solution, and conducting electrostatic spinning, so that the hybrid nanofiber membrane containing P(LLA-CL) and magnesium metal is obtained, and the mass ratio of the P(LLA-CL) to the magnesium metal is (2-10):1. The method is simple, efficient, novel, convenient to operate and good in application prospect; and according to the method, the nanofiber membrane is prepared through electro-spinning of the mixed solution of the P(LLA-CL) and the magnesium powder, and the method is applicable to simulation of fiber degradation performance analysis inside bodies of organisms.
Description
Technical field
The invention belongs to the preparation field of nano fibrous membrane, particularly the electrostatic spinning preparation method of a kind of P (LLA-CL) and magnesium metal mixing nano fibrous membrane.
Background technology
Human tissue injury, damaged meeting cause dysfunction.Traditional restorative procedure is the autograft art, though can obtain satisfactory effect, it is that to sacrifice from the body health tissues be the way of cost, can cause a lot of complication and additional injury; People's organ failure adopt drug therapy, temporary alternative medicine can save part patient life, but the donor organ source is very limited, and because immunological rejection needs the long-term immunodepressant of using, the complication of bringing therefrom is fatal sometimes.After the eighties, scientist proposed " organizational engineering " concept first, for numerous tissue defects, patient's organ failure treatment has brought dawn.In organizational project, cytoskeleton plays effects such as supporting cell growth, guide tissue regeneration, control institutional framework and release active factors, is to determine one of organizational project key of success factor.The degradability of support is paid close attention to, and resists external force and helps the tissue health growth because support should have enough tough mechanicalness.If scaffold degradation speed can be equal to the regeneration rate of tissue, that will be the achievement of making us incomparable excitement.Because with regard to both sides relation, if the too fast function of organization's property that will cause of degradation rate weakens; Otherwise it's low neoblastic the synthesizing of then can hindering is past degradation rate.Traditional large-scale degradable polymer is by very deep research, and conclusion is that the factor that influences degradability has a lot, as sample-size, and molecular wt, degree of crystallinity, purity, temperature, PH and degraded medium etc.
The electrostatic spinning principle is to utilize extra electric field power to make polymer solution or melt overcome surface tension to form jet at the spinning nozzle capillary tip.When electric-field intensity was enough high, under electrostatic repulsion and capillary acting in conjunction, the polymer jet was along unsettled helical trajectory bending motion.Drafted thousands of times in a few tens of milliseconds, with solvent evaporates, jet solidify to form sub-micron to the nano level superfine fiber.The eighties in 20th century, Reneker etc. carried out a large amount of experiments and theory study, had set up comparatively complete technical system.Aspect tissue engineering bracket prepares, have the special advantages support and spin by static and can be endowed the structure that is interweaved and form countless apertures, this structure is the feature of extracellular matrix just also.Have after this architectural feature, various degradable synthetics and natural polymer can be spun in the nano fiber scaffold, are used for various tissue repair and regeneration.
Summary of the invention
Technical problem to be solved by this invention provides the electro-spinning Preparation Method of a kind of P (LLA-CL) and magnesium metal mixing nano fibrous membrane, and this inventive method is simple and direct, efficient, novel and easy to operate, has a good application prospect.
The electro-spinning Preparation Method of a kind of P of the present invention (LLA-CL) and magnesium metal mixing nano fibrous membrane comprises:
Add the magnesium powder in lactic acid caprolactone copolymer P (LLA-CL) solution, dissolving gets electrospinning liquid, carries out electrostatic spinning, namely gets P (LLA-CL) and magnesium metal mixing nano fibrous membrane, and wherein the mass ratio of P (LLA-CL) and magnesium powder is 2-10:1.
The solvent of described P (LLA-CL) solution is hexafluoroisopropanol HFIP.
The concentration of described P (LLA-CL) solution is the 6-10% grams per milliliter.
The mass ratio of described P (LLA-CL) and magnesium powder is 2:1,4:1 or 9:1.
Described electrostatic spinning process parameter is: voltage 14-20 kilovolt, and electric field distance 60-200 millimeter, spinning speed: the 0.5-1.5 milliliter/hour.
Beneficial effect
(1) the inventive method is simple and direct, efficient, novel and easy to operate, has a good application prospect;
(2) the present invention prepares nano fibrous membrane to P (LLA-CL) with magnesium powder mixed solution electrospinning silk exactly, can be applied to the fiber degradation performance evaluation in the simulation organism.
Description of drawings
Fig. 1 is the stereoscan photograph of P (LLA-CL) and magnesium powder nanometer blended fiber film quiet (P (LLA-CL) is 2:1 with the magnesium metal mass ratio) electrospinning silk;
Fig. 2 is the stereoscan photograph of P (LLA-CL) and magnesium powder nanometer blended fiber film quiet (P (LLA-CL) is 9:1 with the magnesium metal mass ratio) electrospinning silk;
Fig. 3 is that the EDS element of P (LLA-CL) and magnesium powder nanometer blended fiber film quiet (P (LLA-CL) is 4:1 with the magnesium metal mass ratio) detects photo.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
With electronic analytical balance take by weighing 0.4 the gram P (LLA-CL) be dissolved in 5 milliliters of hexafluoroisopropanols, obtaining concentration is the 8%(grams per milliliter) P (LLA-CL) solution; Take by weighing 0.2 gram magnesium powder with electronic analytical balance, be dissolved in the 8%(grams per milliliter) P (LLA-CL) solution in, the normal temperature lower magnetic force is stirred to dissolving fully (P in the solution (LLA-CL) is 2:1 with the mass ratio of magnesium powder); Dissolving back solution is electrospinning liquid, and voltage is 16 kilovolts, and the syringe fltting speed is 1.0 milliliters/hour, accepting distance is 15 centimetres, select syringe needle for use No. 9, aluminium-foil paper is accepted, and obtains size and be 10 centimetres x10 centimetre P (LLA-CL) and metal magnesium powder nanometer blended fiber film.
Embodiment 2
With electronic analytical balance take by weighing 0.4 the gram P (LLA-CL) be dissolved in 5 milliliters of hexafluoroisopropanols, obtaining concentration is the 8%(grams per milliliter) P (LLA-CL) solution; Take by weighing 0.1 gram magnesium powder with electronic analytical balance, be dissolved in the 8%(grams per milliliter) P (LLA-CL) solution in, the normal temperature lower magnetic force is stirred to dissolving fully (P in the solution (LLA-CL) is 4:1 with the mass ratio of magnesium powder); Dissolving back solution is electrospinning liquid, and voltage is 14 kilovolts, and the syringe fltting speed is 1.5 milliliters/hour, accepting distance is 15 centimetres, select syringe needle for use No. 9, aluminium-foil paper is accepted, and obtains size and be 10 centimetres x10 centimetre P (LLA-CL) and metal magnesium powder nanometer blended fiber film.
With electronic analytical balance take by weighing 0.4 the gram P (LLA-CL) be dissolved in 5 milliliters of hexafluoroisopropanols, obtaining concentration is the 8%(grams per milliliter) P (LLA-CL) solution; Take by weighing 0.045 gram magnesium powder with electronic analytical balance, be dissolved in the 8%(grams per milliliter) P (LLA-CL) solution in, the normal temperature lower magnetic force is stirred to dissolving fully (P in the solution (LLA-CL) is 9:1 with the mass ratio of magnesium powder); Dissolving back solution is electrospinning liquid, and voltage is 20 kilovolts, and the syringe fltting speed is 0.5 milliliter/hour, accepting distance is 20 centimetres, select syringe needle for use No. 9, aluminium-foil paper is accepted, and obtains size and be 10 centimetres x10 centimetre P (LLA-CL) and metal magnesium powder nanometer blended fiber film.
Claims (5)
1. the electro-spinning Preparation Method of a P (LLA-CL) and magnesium metal mixing nano fibrous membrane comprises:
Add the magnesium powder in lactic acid caprolactone copolymer P (LLA-CL) solution, dissolving gets electrospinning liquid, carries out electrostatic spinning, namely gets P (LLA-CL) and magnesium metal mixing nano fibrous membrane, and wherein the mass ratio of P (LLA-CL) and magnesium powder is 2-10:1.
2. the electro-spinning Preparation Method of a kind of P according to claim 1 (LLA-CL) and magnesium metal mixing nano fibrous membrane, it is characterized in that: the solvent of described P (LLA-CL) solution is hexafluoroisopropanol HFIP.
3. the electro-spinning Preparation Method of a kind of P according to claim 1 (LLA-CL) and magnesium metal mixing nano fibrous membrane, it is characterized in that: the concentration of described P (LLA-CL) solution is the 6-10% grams per milliliter.
4. the electro-spinning Preparation Method of a kind of P according to claim 1 (LLA-CL) and magnesium metal mixing nano fibrous membrane, it is characterized in that: the mass ratio of described P (LLA-CL) and magnesium powder is 2:1,4:1 or 9:1.
5. the electro-spinning Preparation Method of a kind of P according to claim 1 (LLA-CL) and magnesium metal mixing nano fibrous membrane, it is characterized in that: described electrostatic spinning process parameter is: voltage 14-20 kilovolt, electric field distance 60-200 millimeter, spinning speed: the 0.5-1.5 milliliter/hour.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104153124A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible rare-earth oxide nanofiber membrane and preparation method thereof |
| CN104153123A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible titanium oxide nanofiber membrane and preparation method thereof |
| CN104153125A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible ferric oxide nanofiber membrane and preparation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104153124A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible rare-earth oxide nanofiber membrane and preparation method thereof |
| CN104153123A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible titanium oxide nanofiber membrane and preparation method thereof |
| CN104153125A (en) * | 2014-07-30 | 2014-11-19 | 东华大学 | Flexible ferric oxide nanofiber membrane and preparation method |
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Application publication date: 20130814 |