CN1032406C - Medicinal composition with thrombase - Google Patents
Medicinal composition with thrombase Download PDFInfo
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- CN1032406C CN1032406C CN 93114571 CN93114571A CN1032406C CN 1032406 C CN1032406 C CN 1032406C CN 93114571 CN93114571 CN 93114571 CN 93114571 A CN93114571 A CN 93114571A CN 1032406 C CN1032406 C CN 1032406C
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- thrombin
- composition
- compositions
- activity
- colloidal substance
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Abstract
The present invention relates to a new drug composition made by compounding a colloidal substance as an auxiliary material and thrombin. The present invention is characterized in that the activity of the thrombin is evidently enhanced after the colloidal substance is added, and when used, the colloidal substance forms a viscous liquid to protect the activity of the thrombin from damage by liquid and increase the residence time of the thrombin on bleeding wound surfaces, thereby extending drug treating time and enhancing drug effect.
Description
The invention relates to colloidal substance is made a kind of new pharmaceutical composition as adjuvant and thrombin compatibility, after its characteristics are to add colloidal substance, significantly improved the activity of thrombin, make that the thrombin that reaches same curative effect is clinical greatly to be reduced with dosage.
Thrombin is a kind of thrombin in the blood, plays critical effect in the blood coagulation process.In view of the above, as far back as just there being the people that thrombin separation and Extraction from blood is come out as local hemostatic half a century in the past, be applied to the hemorrhage hemostasis of little blood vessel, blood capillary and parenchymal viscera of hemostasis by ligation difficulty.Aspect adjuvant, the producer that has directly with the lyophilization of thrombin extracting solution, does not add any adjuvant; Adding albumin in the thrombin medicinal liquid that has or sucrose are as excipient or proppant, to reduce the loss of activity of thrombin in freezing dry process.
Thrombin is widely used in fields such as Gastroenterology dept., Department of B urn, general surgery, Urology Surgery, plastic surgery, department of obstetrics and gynecology, neurosurgery and department of otorhinolaryngology clinically as a kind of local hemostatic, because thrombin is a kind of protein with styptic activity, lose the part activity so in use can be organized the protease institute's hydrolysis in the liquid inevitably; Particularly aspect the hemostasis of digestive tract hemorrhage, the existence because of gastric acid makes that the pH value of gastric juice is very low in proteolytic enzyme and the gastric juice owing to be rich in the digestive tract, these factors all can influence thrombin activity greatly, and therefore clinical when being used for the digestive tract hemostasis, dosage is all bigger than normal.
Purpose of the present invention is exactly by colloidal substance and thrombin compatibility, improves the activity of thrombin, increases the viscosity of thrombin medicinal liquid simultaneously, thereby increases the residence time of thrombin medicinal liquid at hemorrhage wound surface, to reach the effect that strengthens drug effect; In addition, because the existence of colloidal substance has reduced thrombin and has been organized liquid and the destructive degree of gastric acid, thereby protected thrombin, also reach the effect that strengthens drug effect.
This pharmaceutical composition that contains thrombin is made up of thrombin and animal glue, thrombin and plant gum or thrombin and non-plant and animal material glue, its cooperation ratio is a thrombin: colloid=1000 units: 0.5～2.0 gram, preferred compositions is: thrombin: colloid=1000 units: 0.7～1.8 gram, optimum combination is: thrombin: colloid=1000 monomers: 1.5 grams.Manufacture method is: go out or the ready-made thrombin of buying adds in the medicinal liquid that water is made in 1000 unit thrombins in separation and Extraction: the ratio of colloid 0.5～2.0 gram adds one or more animal glue, plant gum or non-plant and animal material glue etc. as adjuvant, makes dosage forms such as freeze dried powder, powder, tablet, pill, suppository, capsule, ointment.Carry out the determination of activity of thrombin respectively by the method for pharmacopeia regulation then, the result is that activity has all improved 1.5～3.5 times, and the thrombin that confirms to reach same curative effect in zoopery is that consumption has reduced 1.5～3.5 times.
Regard to down and select for use different colloidal substances to be introduced respectively as the optimum implementation of adjuvant:
Example one contains animal glue---gelatin
Get the solution that thrombin 3000 Jia Shui of unit are made into 15ml, add gelatin 3.0 grams again, stirring is made freeze dried powder through degerming processing, lyophilization after making it dissolving.Determining thrombin activity by the method for pharmacopeia regulation then is 4600 units, and activity is increased to 1.5 times.
Example two contains plant gum---and you are gummy for melon
Get the solution that thrombin 3000 Jia Shui of unit are made into 15ml, add your natural gum 2.3 of melon again and restrain, after stirring makes it dissolving, make freeze dried powder through degerming processing, lyophilization, reinstall capsule, the activity of measuring thrombin by official method is 6200 units then, and activity is increased to 2 times.
Example three contains non-animal and plant source glue---hydroxyethyl-cellulose
Get the solution that thrombin 3000 Jia Shui of unit are made into 15ml, add 4.5 gram hydroxyethyl-celluloses again, stirring is made freeze dried powder through degerming processing, lyophilization after making it dissolving, the activity that determines thrombin by official method is 8800 units then, actively improves nearly 3 times.
Claims (10)
1. the pharmaceutical composition that contains thrombin, said composition as adjuvant, is characterized in that described colloid is selected from gelatin, melon that natural gum, hydroxyethyl-cellulose with colloidal substance.
2. according to the compositions of claim l, wherein the ratio of colloidal substance and thrombin is: thrombin: colloid=1000 units: 0.5-2.0g.
3. according to the compositions of claim 1, said composition is a freeze dried powder.
4. according to the compositions of claim 1, said composition is a powder.
5. according to the compositions of claim 1, said composition is a tablet.
6. according to the compositions of claim 1, said composition is a pill.
7. according to the compositions of claim 1, said composition is a suppository.
8. according to the compositions of claim 1, said composition is a capsule.
9. according to the compositions of claim 1, said composition is an ointment.
10. the preparation of drug combination method that contains thrombin, this method comprises: the thrombin of buying that go out in separation and Extraction or ready-made adds in the medicinal liquid that water is made into ratio in 1000 unit thrombin: colloid=0.5-2.0g and adds the colloid that is selected from your natural gum of gelatin, melon, hydroxyethyl-cellulose, makes freeze dried powder, powder, tablet, pill, suppository, capsule or ointment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93114571 CN1032406C (en) | 1992-11-19 | 1993-11-18 | Medicinal composition with thrombase |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92113106.2 | 1992-11-19 | ||
| CN92113106 | 1992-11-19 | ||
| CN 93114571 CN1032406C (en) | 1992-11-19 | 1993-11-18 | Medicinal composition with thrombase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1087545A CN1087545A (en) | 1994-06-08 |
| CN1032406C true CN1032406C (en) | 1996-07-31 |
Family
ID=25742835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93114571 Expired - Fee Related CN1032406C (en) | 1992-11-19 | 1993-11-18 | Medicinal composition with thrombase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1032406C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101137902B (en) * | 2005-03-17 | 2012-04-18 | 积水化学工业株式会社 | Blood coagulation accelerator and vessel for blood test |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181978B (en) * | 2015-09-23 | 2016-07-20 | 青岛古高生物科技有限公司 | A kind of thrombin time test reagent and preparation method thereof |
-
1993
- 1993-11-18 CN CN 93114571 patent/CN1032406C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101137902B (en) * | 2005-03-17 | 2012-04-18 | 积水化学工业株式会社 | Blood coagulation accelerator and vessel for blood test |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1087545A (en) | 1994-06-08 |
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Legal Events
| Date | Code | Title | Description |
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |