CN103239890A - Two-dimensional multi-channel chromatographic system and method for separating and screening strong antioxidant components of pinus koraiensis pinecone extract by using same - Google Patents
Two-dimensional multi-channel chromatographic system and method for separating and screening strong antioxidant components of pinus koraiensis pinecone extract by using same Download PDFInfo
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- CN103239890A CN103239890A CN2013101825492A CN201310182549A CN103239890A CN 103239890 A CN103239890 A CN 103239890A CN 2013101825492 A CN2013101825492 A CN 2013101825492A CN 201310182549 A CN201310182549 A CN 201310182549A CN 103239890 A CN103239890 A CN 103239890A
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Abstract
The invention discloses a two-dimensional multi-channel chromatographic system and a method for separating and screening strong antioxidant components of a pinus koraiensis pinecone extract by using the same, and relates to a chromatographic system and a method for separating and enriching pinecone polyphenol by using the same. According to the system and the method, a novel two-dimensional multi-channel chromatographic system is combined with macrooprous resin for separating and screening the strong antioxidant components of the pinecone polyphenol. The two-dimensional multi-channel chromatographic system is characterized in that a chromatographic column is partitioned into an absorbing column and separating columns, so as to enable a polyphenol compound to be effectively and rapidly separated, and one absorbing column and a plurality of separating columns are adopted for achieving relatively effective separation. Compared with the single-column chromatograph, the two-dimensional multi-channel chromatographic system has the advantages that the polyphenol can be more effectively and rapidly separated, the separating speed can be remarkably increased by a plurality of times even about ten times, the separating efficiency can be remarkably improved by a plurality of times even about ten times, and functional components can be rapidly screened through combining with measurement of antioxidant indexes.
Description
Technical field
The present invention relates to a kind of chromatographic system and utilize it to separate and the method for enrichment pinecone polyphenol, be specifically related to a kind of two-dimentional multichannel chromatographic system and utilize the method for its separation and enrichment pinecone polyphenol.
Background technology
Korean pine is a kind of perennial pinaceae plant that mainly is distributed in Northeast China and neighboring countries and regions.Found that for the research of the extract of pinaceae plant the Pinaceae extract has effects such as anti-oxidant, antitumor, anti-inflammatory, anti-neurodegenerative disease and anti-ultraviolet radiation, being the sky root of Dahurian angelica with the loose skin extract of French maritime pine wherein, research is the most abundant, and the sky root of Dahurian angelica has become the interpolation raw material of food, health products and the cosmetics of international endorsement.
Now in the life facing to a lot of extraneous noxious materials, these Toxic mass-energy cause a large amount of active oxygen of formation in the body, active oxygen can cause the damage of human body, discover that a lot of diseases comprises that tumour, angiocardiopathy etc. are all relevant with the reactive oxygen species in the body, therefore it is necessary reducing activity in vivo oxygen level and improving the interior oxidation resistance of body, and loose extract has very strong oxidation resistance.The main active ingredient of pine extract is components such as polyphenol, flavones and OPC, these components have very strong oxidation resistance, therefore but the contribution to antioxidation activity between the heterogeneity is inconsistent, screens that the highest antioxidant composition is necessary in these compositions.
Macroreticular resin has high adsorbance to polyphenolic substance, is easy to desorption and avirulence, is usually used in separation and the enrichment of polyphenolic substance.Macroreticular resin not only has adsorption effect, but also has capillary effect, molecular sieving effect etc., and effectively classification separates polyphenolic substance.But traditional chromatogram is used monochromatic spectrum column system, and the operating time is long, and efficient is low.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can efficiently separate the two-dimentional multichannel chromatographic system of polyphenolic substance and utilize its separation and the method for screening Korean pine pinecone extract strong anti-oxidation component.
The present invention utilizes two-dimentional multichannel chromatographic system and separates and the strong antioxidant composition of screening pinecone extract in conjunction with macroreticular resin, and concrete steps are as follows:
(1) preparation of pinecone extract: Korean pine pinecone is through 40 ~ 60 ℃ of oven drying 12 ~ 18h, and pulverizer is pulverized, and crosses 20 ~ 50 mesh sieve, be 1:5 ~ 10 according to solid-liquid ratio then, with the ultrasonic auxiliary extraction 0.5 ~ 2h of 40 ~ 80%(v:v) ethanol, repeat merging filtrate 1 ~ 3 time; Rotary evaporation in vacuo instrument concentrated filtrate, the concentration of mensuration polyphenol is used for the optimum macroreticular resin of screening.
(2) two-dimentional multichannel chromatographic system: two-dimentional multichannel chromatographic system is that an independent chromatographic column is divided into adsorption column and splitter, by to adsorption column with splitter is connected and/or wash-out in parallel, thereby realize the efficient of polyphenolic substance separated fast, in order to reach more effective separation, adopt an adsorption column and a plurality of splitter, concrete structure is as follows:
Two dimension multichannel chromatographic system is made of last sample post and several splitters, and sample introduction Guan Junyu goes up the sample post and is connected with splitter, and last sample post connects each splitter successively.
(3) adsorption column washing: will outside post, finish the macroreticular resin of the polyphenol absorption adsorption column of packing into, use the distilled water flushing adsorption column, flow velocity is 10 ~ 200mL/min, remove polyphenolic substance and other impurity of not having absorption, collect a sample every 100 ~ 300mL, polyphenol content in the test sample is zero up to polyphenol content substantially, stops the distillation washing and goes up the sample post.
(4) collection of component: the macroreticular resin that preliminary treatment is the good splitter of packing into, after the adsorption column washing finishes, respectively with 20%, 30%, 40% and 50% ethanol series connection gradient elution adsorption column and splitter, the weight polyphenol fraction that adsorbs on the adsorption column is separated and distributes, elution flow rate is 10 ~ 200mL/min, till dew point occurring to polyphenol, stop to wash sample, the component of each splitter of parallel collecting afterwards, each component rotary evaporation is removed ethanol and is condensed into highly concentrated solution, and to each component freeze drying.
The pine extract has multiple functions such as anti-oxidant, antitumor and radioresistance, and its main component is polyphenol, flavones and OPC etc.Macroreticular resin has high adsorbance, is easy to desorption and avirulence polyphenolic substance, is usually used in the separation of polyphenolic substance.The present invention adopts a kind of new two-dimentional multichannel chromatographic system to separate in conjunction with macroreticular resin and screens loose polyphenol strong anti-oxidation component.Two dimension multichannel chromatographic system is that chromatographic column is divided into adsorption column and splitter, realizes the efficient of polyphenolic substance separated fast, in order to reach more effective separation, adopts an adsorption column and a plurality of splitter.By the screening to the macroreticular resin of 5 kinds of different physicochemical characteristicses, the AB-8 macroreticular resin of finding low pole is optimum separating medium, to the good fit false second order kinetics equation of the absorption result of polyphenolic substance and Langmuir and Freundlich adsorption isotherm equation.Adopt the extract of 0.5-5.0mg/ml polyphenol equivalent as AB-8 macroporous resin absorption solvent, 20,30,40 and 50% ethanolic solution is as constant gradient desorption solvent, the 1/2BV of every splitter collects component as one, and every post is collected 2BV, finally collects 64 components altogether.Measure the ABTS of each component
+Free radical scavenging activity, 50-2.2 has the strongest ABTS as a result
+Secondly free radical scavenging activity is 50-2.3 and 20-2.2.
The present invention is with respect to the single-column chromatogram, can be more efficiently with separate polyphenolic substance fast, its separating rate and efficient can obviously improve several times, even tens times, and by in conjunction with the mensuration of anti-oxidant index screening function component fast.
Description of drawings
Fig. 1 is the basic principle schematic of two-dimentional multichannel chromatographic system, and wherein: X is adsorption column, and F1-F4 is splitter.
The specific embodiment
Below in conjunction with accompanying drawing technical scheme of the present invention is further described; but do not limit to so; every technical solution of the present invention is made amendment or is equal to replacement, and do not break away from the spirit and scope of technical solution of the present invention, all should be encompassed in protection scope of the present invention.
Present embodiment is utilized two-dimentional multichannel chromatographic system according to following steps and is separated and the strong antioxidant composition of screening pinecone extract in conjunction with macroreticular resin:
1) two-dimentional multichannel chromatographic system: chromatographic column is the single-column chromatographic system usually, sample on the single-column upper end, and adopt suitable eluant, eluent to realize the separation of target product.Two dimension multichannel chromatographic system is the last sample district of single-column is separated with the Disengagement zone, and single-column is divided into adsorption column and splitter, and series connection/a plurality of splitters in parallel, realizes the effective separation to target product.Because adsorption column and splitter are connected and are connected in parallel, can realize the series connection wash-out of adsorption column and splitter, simultaneously, also can carry out wash-out independent or in parallel to each independent pillar.Concrete structure is as follows:
Two dimension multichannel chromatographic system is made of adsorption column X and splitter, sample introduction Guan Junyu goes up sample post X and is connected with splitter, described splitter is made up of the first splitter F1, the second splitter F2, the 3rd splitter F3 and the 4th splitter F4, and adsorption column X connects the first splitter F1, the second splitter F2, the 3rd splitter F3 and the 4th splitter F4 successively.
2) preparation of pinecone extract: Korean pine pinecone is removed moisture through 50 ℃ of oven drying 12 ~ 18h, and pulverizer is pulverized, and crosses 30 mesh sieve.Solid-liquid ratio with the ultrasonic auxiliary extraction 1h of 60% ethanol, repeats merging filtrate according to 1:10 2 times.Rotary evaporation in vacuo instrument concentrated filtrate, the concentration of mensuration polyphenol.
3) macroreticular resin preliminary treatment: macroreticular resin is soaked in contains 2 ~ 8h among 1 ~ 5% (wt%) NaOH, use the distilled water flushing resin, up to neutrality; Use 1-5% (wt%) salt acid soak macroreticular resin 2 ~ 8h afterwards, use the distilled water flushing resin, up to neutrality; Use 95%(v:v at last) alcohol immersion macroreticular resin 2 ~ 8h, use the distilled water flushing resin, up to there not being the alcohol flavor; Be soaked in the distilled water.
4) screening of macroreticular resin: the macroreticular resin that preliminary treatment is good, be respectively nonpolar X-5, D101, the AB-8 of low pole and D140 and strong polarity NKA-9 macroreticular resin are used for screening study.Conciliate adsorption rate as the reference index with adsorbance, desorption amount.Adsorption process is the 10-100ml concentrate triangular flask that the pretreated macroreticular resin of 0.5 ~ 5.0g dry weight equivalent places 0.5 ~ 5.0mg/ml polyphenol concentration, and in room temperature, 50 ~ 200rpm shakes 2 ~ 8h, measures not adsorbent solution polyphenol content.Steady-state solution adsorption test program is as follows: when adsorption time reaches, resin is with 10 ~ 200ml distilled water flushing 4 ~ 10 times, and removing not have the polyphenol and the impurity that adsorb, with 10 ~ 100ml (10:90,20:80,30:70,40:60,50:50,60:40,70:30,80:20, v/v) the ethanolic solution gradient desorption is attached, and respectively in room temperature, 50 ~ 200rpm shakes 2 ~ 8h.Rotary evaporation is removed ethanol, measures the polyphenol content of desorb attached liquid.And according to following formula calculating adsorbance, desorption amount reconciliation adsorption rate.
Adsorbance assessment equation:
Q
e = (C
0 C
e)V
i/W
Desorption amount assessment equation:
Q
d = C
dV
d/W
Desorption rate assessment equation:
D= C
dV
d/(C
0 C
e)V
i×100%
Q wherein
eIt is the adsorption capacity (mg polyphenol/g dried resin) when reaching adsorption equilibrium; C
0And C
ePolyphenol concentration (mg/ml) when being respectively the initial and balance of solution; V
iIt is the volume (ml) of sample solution; With W be the weight (g) of dried resin; Q
dBe to reach total desorption ability (mg polyphenol/g dried resin) after the desorption balance at every turn; D is desorption rate (%), C
dBe the total polyphenols concentration (mg/ml) in the desorb attached liquid; V
dIt is the volume (ml) of desorb attached liquid.
The result shows that the AB-8 macroreticular resin of low pole has the highest polyphenol adsorption capacity and conciliates adsorption capacity.
5) operating process of two-dimentional multichannel chromatographic system: the macroreticular resin that preliminary treatment is the good splitter of packing into, will outside post, finish the macroreticular resin of the polyphenol absorption adsorption column of packing into, use the distilled water flushing adsorption column, flow velocity is 30mL/min, up to there not being the polyphenol outflow.Use respectively afterwards
20%, 30%, 40% and 50% ethanol series connection gradient elution adsorption column and splitter, elution flow rate is 30mL/min, dew point appears to polyphenol and till, stop to wash sample, afterwards the component of each splitter of parallel collecting.Each component is the 1/2BV splitter, and each splitter is collected 2BV.And to the name of each component, be example with 20-1.1,20 expression concentration of alcohol, 1 is No. 1 distribution splitter, last 1 be first component in this pillar collection.Each component rotary evaporation is removed ethanol and is condensed into highly concentrated solution, and to each component freeze drying
6) adsorption column washing: on the adsorption column behind the sample, use the distilled water flushing adsorption column, flow velocity is 30mL/min, remove polyphenolic substance and other impurity of not having absorption, collect a sample 1-5mL every 200mL, polyphenol content in the test sample is zero up to polyphenol content substantially, stops distillation washing adsorption column.Therefore the polyphenol that has part in the adsorption column can not need be water-washed away the polyphenol that can not adsorb by the AB-8 macroporous resin adsorption.From the result find out the washing a large amount of polyphenol that can not adsorb of incipient stage washed out, increase along with the washing volume, polyphenol concentration reduces rapidly, when 2000mL, polyphenol concentration in the water lotion almost is reduced to minimum, continues washing again, does not have tangible polyphenol concentration concentration to reduce, can think that water-washing process finishes, so with 2000mL distilled water washing adsorption column.
7) polyphenol dew point: the result finds out from the steady-state solution adsorption experiment, and more polyphenolic substance under 20%, 30%, the 40% and 50% ethanolic solution desorption is in order to simplify experimental implementation and to reduce experimental cost, only with 20%, 30%, 40% and 50% ethanolic solution wash-out pillar.Ethanolic solution series connection gradient elution adsorption column and the splitter of different gradients, flow velocity is 30mL/min, and collect the 1-5ml sample of the last splitter (F4) of series connection every 100mL, polyphenol content in the test sample product, polyphenolic substance obviously appears in sample, stop the wash-out of connecting, and begin single-column collection splitter component in parallel according to operation sequence.
8) ABTS
+The removing activity of free radical: ABTS solution mixing potassium persulfate solution, ratio is 62.5:1, the dark place room temperature is placed 16h and is formed ABTS radical cation mother liquor.ABTS free radical mother liquor distilled water diluting.10 μ L eluant components are mixed the ABTS free-atom aqueous solution of 150 μ L fully, and 6min is placed in the room temperature dark place, and 734nm surveys light absorption value.ABTS
+Free radical is water-soluble external free radical, is usually used in analyzing the oxidation resistance of external water-soluble substances, the electron donation of its bearing reaction polyphenoils.The highest TEAC component is 50-2.2, secondly is 50-2.3 and 20-2.2.
Claims (8)
1. two-dimentional multichannel chromatographic system is characterized in that described system is made of adsorption column (X) and several splitters, and sample introduction Guan Junyu adsorption column (X) is connected with splitter, and adsorption column (X) connects each splitter successively.
2. two-dimentional multichannel chromatographic system according to claim 1, it is characterized in that described splitter is made up of first splitter (F1), second splitter (F2), the 3rd splitter (F3) and the 4th splitter (F4), adsorption column (X) connects first splitter (F1), second splitter (F2), the 3rd splitter (F3) and the 4th splitter (F4) successively.
3. two-dimentional multichannel chromatographic system according to claim 1 and 2, the separating medium that it is characterized in that described adsorption column (X) and splitter is X-5, D101, AB-8, D140 or NKA-9 macroreticular resin.
4. two-dimentional multichannel chromatographic system according to claim 1 and 2, the separating medium that it is characterized in that described adsorption column (X) and splitter is the AB-8 macroreticular resin.
5. one kind is utilized the described two-dimentional multichannel chromatographic system of claim 1 to separate and the method for screening Korean pine pinecone extract strong anti-oxidation component, it is characterized in that described method step is as follows:
(1) preparation of pinecone extract: Korean pine pinecone is through 40 ~ 60 ℃ of oven drying 12 ~ 18h, and pulverizer is pulverized, and crosses 20 ~ 50 mesh sieve, be 1:5 ~ 10 according to solid-liquid ratio then, with the ultrasonic auxiliary extraction 0.5 ~ 2h of 40 ~ 80% (v:v) ethanol, repeat merging filtrate 1 ~ 3 time;
(2) adsorption column washing: will outside post, finish the macroreticular resin of the polyphenol absorption adsorption column of packing into, use the distilled water flushing adsorption column, flow velocity is 10 ~ 200mL/min, remove polyphenolic substance and other impurity of not having absorption, collect a sample every 100 ~ 300mL, polyphenol content in the test sample is zero up to polyphenol content substantially, stops the distillation washing and goes up the sample post;
(3) collection of component: the macroreticular resin that preliminary treatment is the good splitter of packing into, after the adsorption column washing finishes, respectively with 20%, 30%, 40% and 50% ethanol series connection gradient elution adsorption column and splitter, the weight polyphenol fraction that adsorbs on the adsorption column is separated in splitter and distributes, elution flow rate is 10 ~ 200mL/min, till dew point occurring to polyphenol, stop to wash sample, the component of each splitter of parallel collecting afterwards, each component rotary evaporation is removed ethanol and is condensed into highly concentrated solution, and to each component freeze drying.
6. the two-dimentional multichannel chromatographic system of utilization according to claim 5 separates and screens the method for Korean pine pinecone extract strong anti-oxidation component, it is characterized in that described macroreticular resin preprocess method is: macroreticular resin is soaked in 2 ~ 8h among the NaOH, use the distilled water flushing resin, up to neutrality; Use salt acid soak macroreticular resin 2 ~ 8h afterwards, use the distilled water flushing resin, up to neutrality; Use alcohol immersion macroreticular resin 2 ~ 8h at last, use the distilled water flushing resin, up to there not being the alcohol flavor; Be soaked in the distilled water.
7. according to the method for claim 5 or the two-dimentional multichannel chromatographic system separation of 6 described utilizations and screening Korean pine pinecone extract strong anti-oxidation component, it is characterized in that described macroreticular resin is X-5, D101, AB-8, D140 or NKA-9 macroreticular resin.
8. according to the method for claim 5 or the two-dimentional multichannel chromatographic system separation of 6 described utilizations and screening Korean pine pinecone extract strong anti-oxidation component, it is characterized in that described macroreticular resin is the AB-8 macroreticular resin.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104399280A (en) * | 2014-11-26 | 2015-03-11 | 四川九章生物科技有限公司 | Device for enriching and purifying compounds and use method of device |
| CN107132299A (en) * | 2017-06-20 | 2017-09-05 | 哈尔滨工业大学 | A kind of multichannel pack tomographic system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015572A (en) * | 2007-02-07 | 2007-08-15 | 哈尔滨工业大学 | Extraction method of korean pine cone extractive |
| EP2301644A1 (en) * | 2000-06-28 | 2011-03-30 | Novasep Process | Process and device for separating at least one component with a simulated moving bed in columns having an appropriate ratio of length to diameter |
| CN102489042A (en) * | 2011-12-26 | 2012-06-13 | 哈尔滨工业大学 | Two-dimensional chromatography multi-channel separating and purifying apparatus and method |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2301644A1 (en) * | 2000-06-28 | 2011-03-30 | Novasep Process | Process and device for separating at least one component with a simulated moving bed in columns having an appropriate ratio of length to diameter |
| CN101015572A (en) * | 2007-02-07 | 2007-08-15 | 哈尔滨工业大学 | Extraction method of korean pine cone extractive |
| CN102489042A (en) * | 2011-12-26 | 2012-06-13 | 哈尔滨工业大学 | Two-dimensional chromatography multi-channel separating and purifying apparatus and method |
Non-Patent Citations (2)
| Title |
|---|
| 屈东香等: "大孔吸附树脂分离纯化红松松塔水提物", 《哈尔滨医科大学学报》 * |
| 苏晓雨等: "红松种子壳多酚物质的提取及抗氧化特性", 《农业工程学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104399280A (en) * | 2014-11-26 | 2015-03-11 | 四川九章生物科技有限公司 | Device for enriching and purifying compounds and use method of device |
| CN107132299A (en) * | 2017-06-20 | 2017-09-05 | 哈尔滨工业大学 | A kind of multichannel pack tomographic system |
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Application publication date: 20130814 |