CN103232948A - High-temperature resistant saccharomyces cerevisiae strain and breeding method thereof - Google Patents
High-temperature resistant saccharomyces cerevisiae strain and breeding method thereof Download PDFInfo
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Abstract
The invention provides a high-temperature resistant saccharomyces cerevisiae strain and a breeding method of the high-temperature resistant saccharomyces cerevisiae strain. The saccharomyces cerevisiae (Saccharomyces cerevisiae) SY-5-11L has a preservation number of CGMCC No.7377. The strain is characterized in that the ethanol yield is 13.9% (v/v) at a condition of fermenting at high temperature of 38 DEG C on the premise that other fermenting performances are not influenced, the yield is improved by 17.8% as compared with that of a parent strain. The ethanol output at the condition of fermenting at high temperature of 40 DEG C is 12.2% (v/v), and is improved by 34.1% as compared with that of the parent strain. The breeding method comprises the following steps of: performing artificial ultraviolet mutagenesis on an initial strain of the saccharomyces cerevisiae; screening out 23 forward mutant strains as a genome rearrangement library, wherein the ethanol output of the forward mutant strains are obviously raised on the condition of fermenting at a high temperature; and performing the genome rearrangement at three rounds, thus gaining the mutant strains with the maximum ethanol output under the high-temperature fermenting condition. The finally bred saccharomyces cerevisiae strain can be subjected to alcoholic fermentation at the high-temperature fermenting condition, brings no special requirements to fermenting equipment and technological conditions, and can be put into service with the equipment and conditions of general plants, thus having a wide application prospect.
Description
Technical field:
The invention belongs to technical field of bioengineering, relate to the breeding of industrial microorganism, especially the high temperature resistant Wine brewing yeast strain of a strain and selection thereof.
Background technology:
Alcohol occupies very important position in national economy, in medicine, organic synthesis, foodstuffs industry, industrial or agricultural and national defense industry, all has a wide range of applications.Along with continuing to increase of alcohol demand in recent years, the production of alcohol more and more receives people's concern.Chinese Government will " greatly develop fuel alcohol " and list in the Tenth Five-Year Plan (2001-2005) calendar year 2001.In the demonstration to vehicle fuel alcohol " 15 " development ad hoc planning.
Present stage, the raw material of producing alcohol is mainly the corn and other starches raw material, molasses and cellulose raw material etc.; Bacterial strain for fermentation is mainly yeast saccharomyces cerevisiae; The technique adopted is generally thick mash fermentation technique.The industrial scale of alcohol fuel is very huge also comparatively ripe now, yet still exists problem, wherein the most important thing is the rising of the fermentation liquid temperature that causes due to the exothermic reaction in the yeast fermentation process.Too high temperature can produce a lot of adverse influences to breeding and the fermentation of yeast.Thermophilic fermentation has fermenting speed fast, saves the considerable advantages such as water coolant, therefore consider ethanol production process, the wine brewing bacterial strain is transformed, and seed selection heat-resistance type yeast is crucial solution.
Yeast is to the tolerance of the stress conditions such as high temperature by numerous genes and metabolic pathway Collaborative Control, and many regulatory pathways are still not clear, and therefore adopts the molecular breeding technology to carry out genome directional transformation effect to limited target gene limited.Adopt traditional induced-mutation technique to carry out breeding, most yeast strains have produced " passivation " reaction to mutagenic compound, have greatly weakened the effect of traditional breeding technology.Genome rearrangement be a kind of can the quick improvement bacterial strain breeding method of complicated performance, it is based on black box theory, makes a kind of breeding method of genome generation forward mutation optimum combination.Can merge through too much wheel, the forward mutation phenotype is constantly assembled, finally obtain the rearrangement bacterial strain that performance has a distinct increment, than classical selection by mutation, protoplast fusion, there is obvious advantage.Based on this prerequisite, can, by traditional selection by mutation technology is combined with the genome rearrangement technology, select high temperature resistant Wine brewing yeast strain.
Summary of the invention:
The purpose of this invention is to provide the high temperature resistant Wine brewing yeast strain of a strain and selection thereof.
Wine brewing yeast strain provided by the invention is the Wine brewing yeast strain with resistance to elevated temperatures, is specially yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) SY-5-11L.This bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on March 28th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, BeiJing, China city institute, postcode 100101), deposit number is CGMCC No.7377.
Described Wine brewing yeast strain is in the impregnable situation of other leavening property, and under 40 ℃ of fermentation conditions of high temperature, ethanol production is 12.2%(v/v), compare and improved 34.1% than the starting strain ethanol production.
The selection of described Wine brewing yeast strain, specifically can pass through induced mutations starting strain yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC2.0119 (China Committee for Culture Collection of Microorganisms's common micro-organisms center, the public can obtain starting strain 2.0119 by this preservation management committee), filter out some high temperature resistant yeast saccharomyces cerevisiae mutant strains and realize that by efficient three-wheeled living spore hybridization the three-wheel genome rearrangement obtains as ，Jiang Gai library, forward mutant library.
Above-mentioned mutagenesis and genome rearrangement method can obtain by conventional method, these methods have many bibliographical informations, as Lihua Hou, Novel methods of genome shuffling in Saccharomyces cerevisiae[J] .Biotechnology Letters, 2009,31 (5): 671-677.With the molecular breeding method of transformation tolerance genes involved, compare, this method combined by traditional selection by mutation and novel gene group rearrangement set, efficiency and effect all have a clear superiority in; And the mutant strain stability that bacterial strain obtains than genome directional transformation is higher, more is conducive to industrial applications.
The method of the above-mentioned Wine brewing yeast strain of structure provided by the present invention is, first by starting strain through traditional ultraviolet mutagenesis, obtain the various mutant strain colony of genotype; Take 38 ℃ of high-temperature cultivation as the primary dcreening operation condition, sieve bacterial strain with the TTC(triphenyltetrazolium chloride) method carries out multiple sieve, the mutant strain obtained after multiple sieve carries out thermophilic fermentation experiment test producing and ethanol ability again, the final mutant strain promoted to some extent with respect to parent's thermophilic fermentation performance that therefrom filters out some amount, set up the original forward mutant library for genome rearrangement.The genome rearrangement conditions such as the efficient living spore of yeast saccharomyces cerevisiae, hybridization are optimized.Under this condition, the three-wheel genome rearrangement is carried out in original forward mutant library, every take turns rearrangement after, select rearrangement strain that under high temperature, the producing and ethanol ability the is improved forward mutant library as the next round genome rearrangement.The every wheel reset the optimum bacterium of genome rearrangement that screening acquisition ethanol production is the highest under the thermophilic fermentation condition afterwards, the highest Wine brewing yeast strain of ethanol production under acquisition thermophilic fermentation condition after final three-wheel genome rearrangement.
Wine brewing yeast strain of the present invention can be used in wine brewing production.
Beneficial effect:
The present invention utilizes the syngenesis characteristic of brewing yeast cell, amphiploid parent yeast strain is carried out to traditional selection by mutation, to obtain sufficient genetic diversity mutant strain colony, and realize genome rearrangement by the sexual restructuring of many wheels and living spore, build the Wine brewing yeast strain that obtains high-yield ethanol under the thermophilic fermentation condition.This bacterial strain has very high genetic stability, is difficult for producing reverse mutation, and the potential of the suitability for industrialized production of being applied to is arranged.The yeast saccharomyces cerevisiae recombinant bacterial strain CGMCC No.7377 built by present method has the characteristics such as stronger high temperature tolerance and the whole output of higher ethanol, can reduce cooling in fermenting process and later stage and distill required energy consumption, increases economic efficiency.The engineering bacteria that screening obtains does not have particular requirement to fermentation equipment and condition, and equipment and the condition of general factory all can be used, thereby have wide practical use.
The accompanying drawing explanation:
Fig. 1 is the lethality rate curve of ultraviolet mutagenesis.
Fig. 2 is the Technology Roadmap of genome rearrangement process.
Embodiment:
Below by specific embodiment narration the present invention.Unless stated otherwise, in the present invention, technique means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Embodiment 1: the seed selection of high temperature resistant Wine brewing yeast strain
(1) the mutant strain library is set up in traditional selection by mutation
Picking one ring yeast slant culture is inoculated in the YPD liquid nutrient medium of 5mL, and 30 ℃, 100rpm are cultured to the logarithmic growth middle and later periods, and centrifugal collection thalline is also used the stroke-physiological saline solution washed twice, and re-suspended cell is adjusted to 10 by concentration
6individual/mL.Get the 4mL bacteria suspension in Ф 70mm culture dish, use the 15W ultraviolet lamp, irradiated apart from bacterium liquid height 30cm in culture dish, the bacterium liquid after uv irradiating is placed in YPD liquid after 30 ℃, 100rpm are cultivated 4h, is applied on the YPD culture medium flat plate after getting suitable dilution.Bacterium liquid is irradiated the different time to (0s, 5s, 10s, 20s, 30s, 40s, 50s, 60s, 70s, 80s), dilute respectively coated plate and cultivate two days, treat that bacterium colony is longer, to the enumeration on flat board, and calculate lethality rate, draw the lethality rate curve (as shown in Figure 1) based on different irradiation times.Therefore selected 40s is as final mutation time.According to above mutafacient system, cell is carried out to artificial ultraviolet mutagenesis, the bacterium liquid after mutagenesis is applied to the YPD flat board after cultivating 4h and carries out primary dcreening operation through 30 ℃, 100rpm.
By coating YPD flat board after the bacterium liquid dilution obtained after mutagenesis, after the high-temperature cultivation condition is carried out primary dcreening operation, choose wherein larger bacterium colony and carry out multiple sieve in the TTC flat board, by sieve mutant strain be inoculated in the Semen Maydis powder hydrolyzed solution at 40 ℃ of temperature standing for fermentation three days, detect the fermented liquid alcoholic strength.Choose 23 strains and produce the mutant strain of wine degree higher than starting strain, set up the mutant strain library.
The preparation method of described Semen Maydis powder hydrolyzed solution is as follows: the 1500g Semen Maydis powder is added to the tap water of 4500mL65～70 ℃, place 20min, make the abundant water-swelling of Semen Maydis powder particle.Add α-amylase (a-amylase) 0.9mL, 1.5h liquefies under 85～90 ℃.After having liquefied, be cooled to 60 ℃, add saccharifying enzyme 3mL.At 55～60 ℃ of lower saccharification 20h.By the saccharified liquid filter-cloth filtering, obtain clear filtrate, be the corn hydrolyzed solution, boil 10 minutes cooling stand-by.
(2) genome rearrangement
The genome rearrangement technological line as shown in Figure 2.The cell colony in mutant strain library all is inoculated in the YPD liquid nutrient medium, at 30 ℃, is cultured to logarithmic phase under 180rpm, centrifugal collecting cell.With sterilized water, thalline is cleaned, subsequently bacterium mud is linked in pre-product spore substratum (10g/L KAc, 10g/L yeast powder, 20g/L peptone), 28 ℃, 200rpm cultivates 12h, centrifugal collection thalline.After with sterilized water, thalline being washed to three times, gained bacterium mud is inoculated into to efficient liquid and gives birth to spore substratum (10g/L KAc, the 1g/L yeast extract, 0.5g/L glucose, the 50mg/L VITAMIN B4,100mg/L Histidine, 50mg/L uridylic, the 100mg/L tryptophane, the 100mg/L leucine), in, 28 ℃, under 200rpm, cultivate 5d.The bacterium liquid centrifugal collecting cell of efficient living spore process will be completed, helicase liquid with 2% (add 2.5% beta-mercaptoethanol) is resuspended by cell, in 30 ℃, 180rpm processes 12h, centrifugal collection thalline, 0.5%(V/V by equivalent) Triton X-100 is resuspended, by this system of ultrasonic oscillation, make ascus wall fully break and discharge spore, constantly observe during this time broken wall efficiency, in the visual field, during without diploid cell, stop ultrasonication, with sterilized water, thalline is washed three times, is resuspended in 0.5%(V/V) Triton X-100.Certain pityrosporion ovale suspension is applied in the YPD substratum, is placed in 30 ℃, more than 100rpm cultivation 24h, cell is fully hybridized, complete thus first round genome rearrangement.Collecting cell, and it is coated on the YPD flat board, cultivate 24h in 40 ℃ of incubators, the larger bacterium colony of picking from flat board, continue to carry out multiple sieve with the TTC method, the mutant strain obtained after multiple sieve is inoculated in the Semen Maydis powder hydrolyzed solution at 40 ℃ of temperature to standing for fermentation three days, detection fermented liquid alcoholic strength, choose some strains and produce the bacterial strain that the wine degree further improve, for building the second mutant strain library of taking turns genome rearrangement.
According to above-mentioned steps, the cell colony in the mutant strain library that a new round is built is produced spore, spore purifying, hybridization, carry out second and third and take turns genome rearrangement, and, after the third round genome rearrangement, filter out and produce the highest genome rearrangement strain of wine degree under the thermophilic fermentation condition.
Embodiment 2: the leavening property of genome rearrangement bacterial strain
(1) high temperature tolerance of genome rearrangement bacterial strain
Starting strain AY12 and genome rearrangement bacterial strain LYQ-F3 are cultured to logarithmic phase, be seeded to respectively in the Du Shi pipe that malt extract medium (6 ° of Brix) is housed, in the lower standing cultivation of design temperature (30 ℃, 35 ℃, 38 ℃, 40 ℃, 42 ℃), every 12h observes the aerogenesis situation and records result (as shown in table 1) respectively.As shown in the results, after genome rearrangement, the aerogenesis of bacterial strain under hot conditions had obvious lifting than starting strain, shows that the high temperature tolerance of genome rearrangement bacterial strain is higher than starting strain.
The high temperature tolerance of table 1 starting strain and genome rearrangement bacterial strain
Annotate: shown in the data representative result that is experiment more than three times.
(2) fermenting experiment of genome rearrangement bacterial strain
The mutant strain that starting strain and genome rearrangement are selected carries out the ethanol fermentation test simultaneously, measures the relatively producing and ethanol ability of bacterial strain under differing temps, the results are shown in Table 2.As shown in Table 2, when leavening temperature is 30 ℃, the ethanol production of starting strain is alcoholic strength 15.1%(v/v), the ethanol production of mutant strain is alcoholic strength 14.8%(v/v), mutant strain does not have the leavening property advantage; And 38 ℃ with 40 ℃ of thermophilic fermentation conditions under, the ethanol production of mutant strain is respectively 13.9%(v/v) and 12.2%(v/v), than starting strain, improved respectively 17.8% and 34.1%.As can be seen here, Wine brewing yeast strain provided by the invention has the good leavening property of high-yield ethanol under hot conditions.
Table 2 starting strain and mutant strain producing and ethanol (%, v/v) ability are relatively
| |
30℃ | 38 |
40℃ |
| Starting strain | 15.1 | 11.8 | 9.1 |
| Mutant strain | 14.8 | 13.9 | 12.2 |
Annotate: shown in the data mean value that is three parallel test results.
Claims (4)
1. the high temperature resistant Wine brewing yeast strain of a strain, be specially yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) SY-5-11L, and deposit number is CGMCC No.7377.
2. the high temperature resistant Wine brewing yeast strain of a strain as claimed in claim 1, is characterized in that, described high temperature resistant Wine brewing yeast strain ethanol production under 40 ℃ of fermentation conditions of high temperature is 12.2%v/v, than the starting strain ethanol production, compares and has improved 34.1%.
3. the construction process of the high temperature resistant Wine brewing yeast strain of a strain as claimed in claim 1, is characterized in that, concrete steps are as follows: first by starting strain through traditional ultraviolet mutagenesis breeding, obtain the various mutant strain colony of genotype; Take 38 ℃ of high-temperature cultivation as the primary dcreening operation condition, sieve bacterial strain carry out multiple sieve with the TTC method, the mutant strain obtained after multiple sieve carries out thermophilic fermentation experiment test producing and ethanol ability again, finally therefrom filter out the mutant strain that 23 strains promote to some extent with respect to parent's thermophilic fermentation performance, set up the original forward mutant library for genome rearrangement; The three-wheel genome rearrangement is carried out in original forward mutant library, every take turns rearrangement after, select rearrangement strain that under high temperature, the producing and ethanol ability the is improved forward mutant library as the next round genome rearrangement; After final three-wheel genome rearrangement, the highest mutant strain of ethanol production under screening thermophilic fermentation condition, obtain high temperature resistant Wine brewing yeast strain claimed in claim 1.
4. the application of the high temperature resistant Wine brewing yeast strain of a strain as claimed in claim 1 or 2 in wine brewing is produced.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103993042A (en) * | 2014-01-07 | 2014-08-20 | 天津科技大学 | Method for combined production of bioethanol and pullulan from lignocellulose substances |
| CN104371937A (en) * | 2014-10-30 | 2015-02-25 | 江南大学 | Saccharomyces cerevisiae capable of being co-fermented by a plurality of carbon sources and application thereof |
| CN104480030B (en) * | 2014-11-28 | 2017-03-15 | 天津科技大学 | One plant of high temperature resistant Spathaspora passalidarum mutant and its application in xylose fermentation ethanol |
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | The application of saccharomyces cerevisiae and its breeding mode and industrial fermentation production ethanol |
| CN107858298A (en) * | 2017-06-30 | 2018-03-30 | 天津科技大学 | One plant of thermotolerant ethanol fermentation yeast bacterial strain and its structure |
| CN109370929A (en) * | 2018-12-05 | 2019-02-22 | 北京工商大学 | A kind of application of S. cervisiae in wine brewing |
| CN111334442A (en) * | 2018-12-19 | 2020-06-26 | 吉林中粮生化有限公司 | High-temperature-resistant saccharomyces cerevisiae strain and application thereof |
| CN111349592A (en) * | 2020-05-11 | 2020-06-30 | 河南大学 | Spore production culture medium and preparation method of bacterial spores |
| US11060056B2 (en) | 2015-11-25 | 2021-07-13 | Rajendra Surana | Method of producing high amount of ethanol at high temperature by modified yeast strain Saccharomyces cerevisiae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440352A (en) * | 2008-11-17 | 2009-05-27 | 天津科技大学 | High resistance Saccharomyces cerevisiae engineering bacteria and construction method thereof |
| CN101845404A (en) * | 2010-01-08 | 2010-09-29 | 广西科学院 | Brewing yeast strain, breeding method thereof, and application of the strain in alcohol production |
| CN102154138A (en) * | 2011-01-24 | 2011-08-17 | 天津科技大学 | Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof |
-
2013
- 2013-05-10 CN CN201310172490.9A patent/CN103232948B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440352A (en) * | 2008-11-17 | 2009-05-27 | 天津科技大学 | High resistance Saccharomyces cerevisiae engineering bacteria and construction method thereof |
| CN101845404A (en) * | 2010-01-08 | 2010-09-29 | 广西科学院 | Brewing yeast strain, breeding method thereof, and application of the strain in alcohol production |
| CN102154138A (en) * | 2011-01-24 | 2011-08-17 | 天津科技大学 | Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 吴帅等: "高耐性酿酒酵母菌种的筛选", 《酿酒科技》 * |
| 王英等: "蓝莓自然发酵酒中优良酵母菌的筛选及鉴定", 《中国食品学报》 * |
| 苏艳秋 等: "耐高温、耐酸产酒精酵母的筛选与鉴定", 《微生物学杂志》 * |
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| CN103993042B (en) * | 2014-01-07 | 2016-08-24 | 天津科技大学 | A kind of lignocellulose material co-producing bio ethanol and the method for pulullan |
| CN103993042A (en) * | 2014-01-07 | 2014-08-20 | 天津科技大学 | Method for combined production of bioethanol and pullulan from lignocellulose substances |
| CN104371937A (en) * | 2014-10-30 | 2015-02-25 | 江南大学 | Saccharomyces cerevisiae capable of being co-fermented by a plurality of carbon sources and application thereof |
| CN104480030B (en) * | 2014-11-28 | 2017-03-15 | 天津科技大学 | One plant of high temperature resistant Spathaspora passalidarum mutant and its application in xylose fermentation ethanol |
| US11060056B2 (en) | 2015-11-25 | 2021-07-13 | Rajendra Surana | Method of producing high amount of ethanol at high temperature by modified yeast strain Saccharomyces cerevisiae |
| CN107858298A (en) * | 2017-06-30 | 2018-03-30 | 天津科技大学 | One plant of thermotolerant ethanol fermentation yeast bacterial strain and its structure |
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | The application of saccharomyces cerevisiae and its breeding mode and industrial fermentation production ethanol |
| CN107603898B (en) * | 2017-10-26 | 2019-06-28 | 中国科学院天津工业生物技术研究所 | The application of saccharomyces cerevisiae and its breeding mode and industrial fermentation production ethyl alcohol |
| CN109370929A (en) * | 2018-12-05 | 2019-02-22 | 北京工商大学 | A kind of application of S. cervisiae in wine brewing |
| CN109370929B (en) * | 2018-12-05 | 2022-06-17 | 北京工商大学 | Application of saccharomyces cerevisiae in brewing wine |
| CN111334442A (en) * | 2018-12-19 | 2020-06-26 | 吉林中粮生化有限公司 | High-temperature-resistant saccharomyces cerevisiae strain and application thereof |
| CN111334442B (en) * | 2018-12-19 | 2022-10-25 | 吉林中粮生化有限公司 | High-temperature-resistant saccharomyces cerevisiae strain and application thereof |
| CN111349592A (en) * | 2020-05-11 | 2020-06-30 | 河南大学 | Spore production culture medium and preparation method of bacterial spores |
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Effective date of registration: 20220328 Address after: 563000 Maotai Town, Renhuai City, Zunyi City, Guizhou Province Patentee after: Guizhou Diaoyutai State Guest Wine Co.,Ltd. Address before: 300457 No. 29, 13th Street, Tanggu economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: TIANJIN University OF SCIENCE AND TECHNOLOGY |