CN103232891A - Method for fermenting and preparing tobacco flavor by using magnetically-stabilized fluidized bed - Google Patents
Method for fermenting and preparing tobacco flavor by using magnetically-stabilized fluidized bed Download PDFInfo
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- CN103232891A CN103232891A CN2013101683523A CN201310168352A CN103232891A CN 103232891 A CN103232891 A CN 103232891A CN 2013101683523 A CN2013101683523 A CN 2013101683523A CN 201310168352 A CN201310168352 A CN 201310168352A CN 103232891 A CN103232891 A CN 103232891A
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- 241000208125 Nicotiana Species 0.000 title claims abstract description 24
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000000796 flavoring agent Substances 0.000 title abstract 12
- 235000019634 flavors Nutrition 0.000 title abstract 12
- 239000007788 liquid Substances 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000001301 oxygen Substances 0.000 claims abstract description 7
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 7
- 230000000391 smoking effect Effects 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims description 46
- 230000006641 stabilisation Effects 0.000 claims description 45
- 238000011105 stabilization Methods 0.000 claims description 45
- 235000013599 spices Nutrition 0.000 claims description 23
- 108010059820 Polygalacturonase Proteins 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 7
- 238000002203 pretreatment Methods 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 4
- 108010010803 Gelatin Proteins 0.000 claims description 3
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 3
- XZKRXPZXQLARHH-UHFFFAOYSA-N buta-1,3-dienylbenzene Chemical compound C=CC=CC1=CC=CC=C1 XZKRXPZXQLARHH-UHFFFAOYSA-N 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000007334 copolymerization reaction Methods 0.000 claims description 3
- 239000011258 core-shell material Substances 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 229940098465 tincture Drugs 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 9
- 102000004190 Enzymes Human genes 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 238000000855 fermentation Methods 0.000 abstract description 6
- 230000004151 fermentation Effects 0.000 abstract description 6
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 239000003205 fragrance Substances 0.000 abstract description 4
- 235000010987 pectin Nutrition 0.000 abstract 3
- 229920001277 pectin Polymers 0.000 abstract 3
- 239000001814 pectin Substances 0.000 abstract 3
- 238000007781 pre-processing Methods 0.000 abstract 2
- 206010013911 Dysgeusia Diseases 0.000 abstract 1
- 235000019504 cigarettes Nutrition 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 238000005243 fluidization Methods 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 6
- 239000007789 gas Substances 0.000 description 3
- 210000000697 sensory organ Anatomy 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 230000023556 desulfurization Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003500 flue dust Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005987 sulfurization reaction Methods 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
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Abstract
The invention discloses a method for fermenting and preparing tobacco flavor by using a magnetically-stabilized fluidized bed. The method comprises the following steps of: (1) washing and sterilizing the inside of the magnetically-stabilized fluidized bed and setting a pectin enzyme reaction condition under a state in which a magnetic field is closed; (2) preprocessing a magnetic sensitive micro-carrier and the pectin enzyme, and subsequently adding the magnetic sensitive micro-carrier and the pectin enzyme; (3) preprocessing the flavor, uniformly stirring the flavor by using water and then adding the flavor; (4) filling sterile air or oxygen-enriched air till a suspension state occurs, then opening the magnetic field and stopping filling the air; and (5) sampling at a material liquid outlet in time, quantitatively adding the fermented and prepared tobacco flavor in tobaccos and determining whether reserving the samples or carrying out magnetically-stabilized fluidization treatment again according to smoking assessment results. By adopting the magnetically-stabilized fluidized bed to ferment and prepare the tobacco flavor, the method for fermenting and preparing tobacco flavor by using the magnetically-stabilized fluidized bed has the advantages of accelerating growth and activity of the enzyme, enlarging a roughness and a contact surface between the enzyme and the flavor surface under impact of airflow and magnetic action, improving the fermentation rate of the flavor, adding the flavor in the cigarettes and improving the fragrance, concealing miscellaneous flavor and improving aftertaste.
Description
Technical field
The present invention relates to the preparation method of tobacco aromatics using, refer to a kind of method of utilizing magnetic stabilization fluid bed fermentative preparation tobacco aromatics using particularly.
Background technology
At present, tobacco aromatics using mainly utilizes immobilized enzyme reactor to come fermentative preparation.Traditional immobilized enzyme reactor adopts fixed bed more, and it is big to exist axial pressure drop, and is easily broken in operation for tiny, surperficial nonrigid carrier; Easily stop up bed for high viscosity substrate and Powdered substrate, temperature, pH value are not easy to shortcomings such as control.
Magnetic stabilization fluid bed is to add magnetic field to common fluidized-bed, and the particle in the fluidized-bed also has magnetic field.Particle forms chain in magnetic field, liquid during by fluidized-bed granular layer evenly expand, liquid is plug-flow.With respect to centrifugal field, oscillator field harmony field fluidized-bed, magnetic stabilization fluid bed have little, a advantage that noise is little of vibration, integrates the advantage of fixed bed and traditional fluidized-bed simultaneously; Solid particulate is easy to loading and unloading and can be recycled high mass transfer, rate of heat transfer etc.
At present magnetic stabilization fluid bed be mainly used in the chemical industry environmental protection with dispose of sewage, flue dust desulfurization etc.; Be used for biochemical engineering with separation and purification protein, cultivation zooblast etc.But for not appearing in the newspapers so far with the stable sulfuration bed of magnetic fermentative preparation tobacco aromatics using.
Summary of the invention
Purpose of the present invention will overcome the existing in prior technology deficiency exactly, and a kind of method of utilizing magnetic stabilization fluid bed fermentative preparation tobacco aromatics using is provided.
The present invention utilizes the method for magnetic stabilization fluid bed fermentative preparation tobacco aromatics using, may further comprise the steps:
1) under the closing state of magnetic field, all containers in magnetic stabilization fluid bed, pipeline are cleaned, sterilize, before startup was magnetic stabilization fluid bed, setting the required solution pH value of polygalacturonase was 6.0, and dissolved oxygen is 30% air saturation, and temperature of reaction is 45 ℃;
2) with after Magnetosensitive microcarrier and the polygalacturonase pre-treatment, join in sterilized magnetic stabilization fluid bed, wherein, described Magnetosensitive microcarrier is the core-shell type structure, and kernel is by the Fe of 50 μ m
3O
4Powder and vinylbenzene and Vinylstyrene copolymerization form, and shell is gelatin, Fe
3O
4Powder accounts for 40% of Magnetosensitive microcarrier weight; The add-on of pretreated Magnetosensitive microcarrier is by volume measured and is 10%～30% of magnetic stabilization fluid bed working volume, and the coercive force of pretreated Magnetosensitive microcarrier is lower than 150 amperes per meter, and particle diameter is 500 μ m～2mm, and magneticstrength is 10～100mT;
3) water with 40～60 ℃ after the spices pre-treatment is stirred, again the spice solution of gained is joined magnetic stabilization fluid bed in, and the pretreated Magnetosensitive microcarrier of submergence but be no more than 90% of magnetic stabilization fluid bed working volume;
4) feed sterile air or oxygen-rich air, pretreated Magnetosensitive microcarrier is fully mixed with spice solution, until being suspended state, open magnetic field then, stop ventilation;
5) in time take a sample at material liquid outlet, and will be according to the method described above the tobacco aromatics using of fermentative preparation in pipe tobacco, quantitatively apply, according to smoking result, determine whether to keep sample or carry out magnetic stabilization fluid again and handle.
In step 2) in, embedding or surface modifying method are altogether adopted in described Magnetosensitive microcarrier and polygalacturonase pre-treatment, promote the Fe of polygalacturonase growth
3O
4The effective add-on of powder is 1.0～6.0 g/L.
In step 3), described spices pretreatment process is made medicinal extract or tincture after spices is ground into solid particulate or powder.
Beneficial effect of the present invention is: use the problem that magnetic stabilization fluid bed fixing bio-reactor can effectively be avoided traditional immobilized enzyme reactor, enzyme or other biological active substance are fixed on the magnetosensitive carrier and form immobilized enzyme, under the action of a magnetic field, can enlarge operating restraint, improve mass-transfer efficiency.Can also utilize mode of motion and the direction of external magnetic field control magneticsubstance immobilized enzyme in addition, substitute traditional mechanical stirring mode, improve the catalytic efficiency of immobilized enzyme.
Utilize magnetic stabilization fluid bed fermentative preparation tobacco aromatics using, can improve the fermentation rate of spices.Magnetosensitive microcarrier in magnetic stabilization fluid bed can promote growth and the activity of enzyme under the effect in magnetic field; Under the impact and magnetic action of air-flow, strengthen roughness and the contact surface on enzyme and spices surface, increase the attachment point of enzyme, accelerate the effect of enzyme and the fermentation of spices.Utilize the spices after the magnetic stabilization fluid bed processing, exceed 1.0～1.5 fens than the sense organ score value of the spices that is untreated, be added on fragrance is promoted to some extent, can cover assorted gas and improve pleasant impression.
Description of drawings
Fig. 1 is magnetic stabilization fluid bed structural representation of the present invention.
Embodiment
The present invention is described in further detail below in conjunction with the drawings and specific embodiments.
Used Magnetosensitive microcarrier is the core-shell type structure, and kernel is by the Fe of 50 μ m
3O
4Powder and vinylbenzene and Vinylstyrene copolymerization form, and shell is gelatin, Fe
3O
4Powder accounts for 40% of Magnetosensitive microcarrier weight.
As shown in Figure 1, in the magnetic stabilization fluid bed main body 1 of the present invention Magnetosensitive microcarrier 2 is housed, adds the longitudinal magnetic field that direction makes progress common in liquid-solid fluid bed, realize with solenoid 3 in magnetic field, logical direct current in the coil.Magnetic field can be uniform magnetic field, also can be the magnetic field that gradient is arranged.The top of magnetic stabilization fluid bed main body 1 is the taper expanding reach, and purpose is that speed reduces after making spices after the fermentation leave granular layer, reduces particle entrainment; The bottom of magnetic stabilization fluid bed main body 1 is the taper section of dwindling downwards, eliminates the dead angle, thereby improves the fermentation rate of spices.The side on magnetic stabilization fluid bed main body 1 top is provided with spices outlet 8, and the side at middle part is provided with detects mouthfuls 9, detects mouthfuls 9 and is connected with dissolved oxygen sensor with solution pH value sensor, temperature sensor.The upper surface on magnetic stabilization fluid bed main body 1 top is provided with venting port 10 and microcarrier adds entrance 11, sterilize the respectively discharge of steam and the adding of Magnetosensitive microcarrier.The upper end of magnetic stabilization fluid bed main body 1 bottom is provided with filter screen 4, to prevent particle seepage.Filter screen 4 has liquid distributing board 5, and liquid is evenly distributed.The lower end of magnetic stabilization fluid bed main body 1 bottom is provided with sterilization steam-in 6 and spices entrance 7; Sterilization steam enters the sterilising treatment of a body before fermenting from sterilization steam-in 6; Pretreated spices enters fluidized-bed from the spices entrance 7 of bed body lower end and ferments.
Embodiment 1
Utilize the method for magnetic stabilization fluid bed fermentative preparation tobacco aromatics using, may further comprise the steps:
1) under the closing state of magnetic field, all containers in magnetic stabilization fluid bed, pipeline are cleaned, sterilize, before startup was magnetic stabilization fluid bed, setting the polygalacturonase pH value was 6.0, and dissolved oxygen is 30% air saturation, and temperature of reaction is 45 ℃;
2) Magnetosensitive microcarrier and polygalacturonase are adopted embedding method pre-treatment altogether, promote the Fe of polygalacturonase growth
3O
4The effective add-on of powder is 4.0 g/L, 2.0 kilograms of pretreated Magnetosensitive microcarriers are joined in sterilized magnetic stabilization fluid bed, wherein, the add-on of pretreated Magnetosensitive microcarrier is by volume measured and is 15% of magnetic stabilization fluid bed working volume, the coercive force of pretreated Magnetosensitive microcarrier is lower than 150 amperes per meter, particle diameter is 500 μ m, and magneticstrength is 50mT;
3) red date is extracted red date medicinal extract after crushed, again the water of 50 kilograms of red date medicinal extract with 45 ℃ is stirred, again the red date medicinal extract solution of gained is joined magnetic stabilization fluid bed in, and the pretreated Magnetosensitive microcarrier of submergence but be no more than 90% of magnetic stabilization fluid bed working volume;
4) feed sterile air, pretreated Magnetosensitive microcarrier is fully mixed with red date medicinal extract solution, until being suspended state, open magnetic field then, stop ventilation, pretreated Magnetosensitive microcarrier is under the effect in magnetic field, can promote growth and the activity of polygalacturonase, under the impact and magnetic action of air-flow, strengthen roughness and the contact surface on polygalacturonase and red date medicinal extract surface, increase the attachment point of polygalacturonase, accelerated the effect of polygalacturonase and the fermentation of red date medicinal extract;
5) after magnetic field acts on 1h respectively, behind the 1.5h, in time take a sample at material liquid outlet respectively, and in the blank pipe tobacco of certain trade mark, evenly apply according to 0.05% of pipe tobacco gross weight, according to smoking result, determine whether to keep sample or carry out magnetic stabilization fluid again and handle, and with apply relative quantity do not have the red date medicinal extract handled to the contrast that follows the pattern of, adopt sensory quality assessment to give a mark (the gloss branch is disregarded), it the results are shown in table 1.
The sense organ rating result of table 1 spices
| Sample | Treatment time | Fragrance | Coordinate | Assorted gas | Stimulate | Pleasant impression | Add up to |
| To in the same old way | — | 28.5 | 5.0 | 11.0 | 18.0 | 22.0 | 84.5 |
| The test sample | 1h | 29.0 | 5.0 | 11.5 | 18.0 | 22.5 | 86.0 |
| The test sample | 1.5h | 28.5 | 5.0? | 11.5? | 18.0? | 22.5? | 85.5 |
Smoking result shows, utilize magnetic stabilization fluid bed processing after, exceed 1.0～1.5 fens than the sense organ score value of the spices that is untreated, mainly be that fragrance promotes to some extent, can cover assorted gas and improve pleasant impression, handle the 1h better effects if in the present embodiment.
Claims (3)
1. a method of utilizing magnetic stabilization fluid bed fermentative preparation tobacco aromatics using is characterized in that, may further comprise the steps:
1) under the closing state of magnetic field, all containers in magnetic stabilization fluid bed, pipeline are cleaned, sterilize, before startup was magnetic stabilization fluid bed, setting the required solution pH value of polygalacturonase was 6.0, and dissolved oxygen is 30% air saturation, and temperature of reaction is 45 ℃;
2) with after Magnetosensitive microcarrier and the polygalacturonase pre-treatment, join in sterilized magnetic stabilization fluid bed, wherein, described Magnetosensitive microcarrier is the core-shell type structure, and kernel is by the Fe of 50 μ m
3O
4Powder and vinylbenzene and Vinylstyrene copolymerization form, and shell is gelatin, Fe
3O
4Powder accounts for 40% of Magnetosensitive microcarrier weight; The add-on of pretreated Magnetosensitive microcarrier is by volume measured and is 10%～30% of magnetic stabilization fluid bed working volume, and the coercive force of pretreated Magnetosensitive microcarrier is lower than 150 amperes per meter, and particle diameter is 500 μ m～2mm, and magneticstrength is 10～100mT;
3) water with 40～60 ℃ after the spices pre-treatment is stirred, again the spice solution of gained is joined magnetic stabilization fluid bed in, and the pretreated Magnetosensitive microcarrier of submergence but be no more than 90% of magnetic stabilization fluid bed working volume;
4) feed sterile air or oxygen-rich air, pretreated Magnetosensitive microcarrier is fully mixed with spice solution, until being suspended state, open magnetic field then, stop ventilation;
5) in time take a sample at material liquid outlet, and will be according to the method described above the tobacco aromatics using of fermentative preparation in pipe tobacco, quantitatively apply, according to smoking result, determine whether to keep sample or carry out magnetic stabilization fluid again and handle.
2. the method for utilizing magnetic stabilization fluid bed fermentative preparation tobacco aromatics using according to claim 1 is characterized in that: in step 2) in, embedding or surface modifying method are altogether adopted in described Magnetosensitive microcarrier and polygalacturonase pre-treatment, promote the Fe of polygalacturonase growth
3O
4The effective add-on of powder is 1.0～6.0 g/L.
3. the method for utilizing magnetic stabilization fluid bed fermentative preparation tobacco aromatics using according to claim 1 is characterized in that: in step 3), described spices pretreatment process is made medicinal extract or tincture after spices is ground into solid particulate or powder.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593139A (en) * | 2015-01-21 | 2015-05-06 | 东北农业大学 | Method for carrying out enzymolysis on soybean by using gas-solid two-phase magnetically-stabilized fluidized bed |
| CN104629891A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Research on method for extracting oil and fat from soybeans through enzymolysis by using gas-liquid-solid three-phase magnetically-stabilized fluidized bed |
| CN110684655A (en) * | 2019-10-18 | 2020-01-14 | 江苏大学 | Microalgae separation gradient magnetic stabilization fluidized bed device and microalgae harvesting method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104629891A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Research on method for extracting oil and fat from soybeans through enzymolysis by using gas-liquid-solid three-phase magnetically-stabilized fluidized bed |
| CN110684655A (en) * | 2019-10-18 | 2020-01-14 | 江苏大学 | Microalgae separation gradient magnetic stabilization fluidized bed device and microalgae harvesting method thereof |
| CN110684655B (en) * | 2019-10-18 | 2023-03-17 | 江苏大学 | Microalgae separation gradient magnetic stabilization fluidized bed device and microalgae harvesting method thereof |
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| CN103232891B (en) | 2014-12-17 |
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