CN103224280A - Sterilization device and method for microbe fermentation water - Google Patents
Sterilization device and method for microbe fermentation water Download PDFInfo
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- CN103224280A CN103224280A CN2013101855587A CN201310185558A CN103224280A CN 103224280 A CN103224280 A CN 103224280A CN 2013101855587 A CN2013101855587 A CN 2013101855587A CN 201310185558 A CN201310185558 A CN 201310185558A CN 103224280 A CN103224280 A CN 103224280A
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Abstract
The invention discloses a sterilization device for microbe fermentation water, which comprises a water treatment system and an ozone set, wherein the water treatment system comprises a water storage tank; a first liquid outlet pipe communicated with a gas-liquid mixing pump and a sterile pipeline communicated with a fermentation tank are arranged below the water storage tank; a liquid outlet of the gas-liquid mixing pump is provided with a second liquid outlet pipe communicated with a gas-liquid separation tank; one side of the gas-liquid separation tank is provided with a third liquid outlet pipe communicated with the water storage tank; and the ozone set comprises an oxygenerator and an ozone machine communicated with the oxygenerator through a pipeline. Besides, the invention also discloses a method for sterilizing microbe fermentation water by using the device. When being used for sterilizing the microbe fermentation water, the device and method satisfy the requirement of pure culture fermentation of microbes, saves the steam consumption required by culture medium sterilization, and has the advantages of low energy consumption, no pollution, high economy and high reliability.
Description
Technical field
The invention belongs to the microbial fermentation technology field, be specifically related to a kind of sterilization device and method of microbial fermentation water.
Background technology
Traditional fermentation culture medium for microbe constant volume water sterilization is to utilize the heat energy of saturation steam to come killing microorganisms, thereby obtains aseptic culture medium, and its disadvantage is the requirement harshness to the quality and quantity of steam, and power consumption is high, pollution is high.And the good sterilizing function that ozone had can be killed the microorganism that survives in the tap water, traditional ozone preparation efficiency is low, shortcoming is obvious, mainly show as and produce that ozone purity is low, the air water mixing efficiency is low, that sterilization does not thoroughly reach gas distribution is irregular etc., and floor space is big, investment is expensive.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of sterilization device of microbial fermentation water at above-mentioned the deficiencies in the prior art.That this equipment drops into is less, power consumption is low, pollution-free, save the space, has solved shortcomings such as power consumption height, sterilization time that steam sterilizing brings be long, overcome simultaneously traditional ozone water sterilization not thoroughly, can not be used for the shortcoming that microbial fermentation is produced.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of sterilization device of microbial fermentation water is characterized in that this equipment comprises water treatment system and ozone unit; Described water treatment system comprises water tank, and the below of described water tank is provided with and is connected with gas-liquid mixing pump and is used for aseptic pipeline that the water of water tank is sent into first drain pipe in the gas-liquid mixing pump and is connected with fermentor tank; The liquid outlet of described gas-liquid mixing pump is provided with second drain pipe that is connected with knockout drum, the top of described knockout drum is provided with first escape pipe that is connected with exhaust gas treating device and is used for gas separated in the knockout drum is sent into exhaust gas treating device, one side of described knockout drum is provided with the 3rd drain pipe that is connected with water tank and is used for isolating liquid in the knockout drum is sent into water tank, be provided with ozone eliminator in described first drain pipe, described the 3rd drain pipe is provided with the ozone concn detector, direction from ozone eliminator to gas-liquid mixing pump on described first drain pipe is disposed with first valve and vacuum meter, and the direction from the ozone concn detector to water tank on described the 3rd drain pipe is disposed with first tensimeter and second valve;
Described ozone unit comprises oxygenerator, with the ozone machine that is connected with oxygenerator by pipeline, be disposed with the oxygen flow meter and second tensimeter on the pipeline between described oxygenerator and the ozone machine, the air outlet of described ozone machine is provided with second escape pipe that is connected with gas-liquid mixing pump and is used for ozone is sent into gas-liquid mixing pump, and described second escape pipe is provided with stopping valve.
The sterilization device of above-mentioned a kind of microbial fermentation water, described ozone eliminator is by being installed on the ultraviolet lamp in first drain pipe and being installed in first drain pipe and the strainer that is positioned at the ultraviolet lamp right side is formed.
The sterilization device of above-mentioned a kind of microbial fermentation water, the wavelength of the emission light of described ultraviolet lamp is 240nm～260nm.
The sterilization device of above-mentioned a kind of microbial fermentation water, described oxygenerator are the PSA oxygen generator.
The sterilization device of above-mentioned a kind of microbial fermentation water, described gas-liquid mixing pump are the Ni Keni pump.
The sterilization device of above-mentioned a kind of microbial fermentation water, described ozone machine are the corona-type ozone machine.
In addition, the present invention also provides a kind of aforesaid device that adopts to the method that the microbial fermentation water carries out disinfection and sterilizes, and it is characterized in that this method may further comprise the steps:
Step 1, close the aseptic pipeline of water tank, close ozone eliminator, in water tank, inject water, open gas-liquid mixing pump, water flows in the gas-liquid mixing pump from water tank by first drain pipe, thereby and the opening degree of controlling first valve velocity of flow of regulating water in first drain pipe to make the vacuum tightness of vacuum meter be 0.005MPa～0.015MPa;
Step 2, startup oxygenerator system oxygen, regulate oxygen flow, treat that the second manometric pressure reaches 0.015MPa～0.030MPa, start ozone machine behind maintenance 3min～5min and prepare ozone, open stopping valve simultaneously the ozone of preparation is entered in the gas-liquid mixing pump by second escape pipe;
Step 3, the ozone that enters gas-liquid mixing pump in the water that flows into gas-liquid mixing pump in the step 1 and the step 2 mixes in gas-liquid mixing pump, utilize ozone that water is carried out disinfection, mixed gas-liquid mixture enters and carries out gas-liquid separation in the knockout drum, open second valve, gas after the separation is discharged after exhaust gas treating device is handled, water after the separation enters after the ozone concn detector detects and finishes once circulation in the water tank, the flow that enters the ozone in the gas-liquid mixing pump by stopping valve control makes the concentration of the ozone in water after separating maintain 2.5mg/L～3.0mg/L, and is keeping under the constant situation of this concentration water being carried out circular treatment; Time T=the V/Q of described circular treatment, wherein V is the volume of the water of injection water tank, Q is the volumetric flow rate of water;
Step 4, treat that circular treatment described in the step 3 finishes after, close stopping valve, close ozone machine then, then close oxygenerator, open ozone eliminator, when keeping water in pipeline, to be circulated to the detected ozone in water concentration of ozone concn detector being 0mg/L, open aseptic pipeline, close first drain pipe simultaneously, make the water after the circular treatment inject fermentor tank as fermention medium constant volume water by aseptic pipeline.
The present invention compared with prior art has the following advantages:
1, equipment input of the present invention is less, power consumption is low, pollution-free, floor space is little, save space, easy to operate, solved shortcomings such as power consumption height, sterilization time that steam sterilizing brings be long, overcome simultaneously traditional ozone water sterilization not thoroughly, can not be used for the shortcoming that microbial fermentation is produced.
2, the present invention adopts the unstripped gas of the oxygen of oxygenerator generation as ozone machine, the ozone concn height that obtains, the oxynitride that has produced when having avoided simultaneously using air source.
3, the preferred corona-type ozone machine of ozone machine of the present invention has the efficient height, volume is little, high frequency characteristics good, characteristics such as working stability; The preferred Ni Keni pump of gas-liquid mixing pump, good mixing effect can promote the dissolving of ozone in water to greatest extent, sterilising effect is good; Gas-liquid mixing pump mixes the remaining ozone gas in back and decomposes non-secondary pollution through exhaust gas treating device in real time.
4, the present invention adopts remaining ozone in the water after ozone eliminator is eliminated sterilization, has avoided the destruction of residual ozone to fermention medium and target bacterial classification; In addition, the ozone eliminator that ozone eliminator preferred filters and ultraviolet lamp are formed, ultraviolet lamp has played the effect of auxiliary sterilization again when ozone is further eliminated, but impurity and be killed the corpse of bacterium in the strainer Filtration Adsorption water.
5, the present invention has utilized two circulation lines: the one, and system ozone water step cycle, between water treatment system and ozone unit, circulate, make the interior gas of gas-liquid mixing pump, liquid two phase pressures constantly be in mixed-balance-imbalance-mixed again state, thereby improved the mixed efficient of gas-liquid; The 2nd, the circulation of ozone maintenance stage utilizes the transmission of gas-liquid mixing pump, and ozone water is carried out a jar Outer Tube self-circulation, has further strengthened the micro-interface contact of gas-liquid, thereby has kept the concentration of ozone in water, reaches thorough sterilization purpose.
6, the ozone-containing sterilizing water behind the employing sterilization of the present invention carries out constant volume to sterilized basic medium in the fermentor tank, gauge pressure is the blank 48h of cultivation under the condition of 0.05MPa～0.06MPa in 37 ℃, jars, the 6h sampling of basic medium behind constant volume interval is checked, the result shows in each stage sample does not all have varied bacteria growing, illustrates that ozone-containing sterilizing water of the present invention can satisfy the requirement of microorganism pure-blood ferment.
7, adopt equipment of the present invention and method to the sterilization that carries out disinfection of microbial fermentation water, both satisfied the requirement of microorganism pure-blood ferment, the consumption of required steam when having saved the substratum sterilization again, it is low, pollution-free to consume energy, economic and reliable.
Below in conjunction with drawings and Examples, technical scheme of the present invention is described in further detail.
Description of drawings
Fig. 1 is the structural representation of present device.
Fig. 2 is the structural representation of ozone eliminator of the present invention.
Description of reference numerals:
1-water tank; 1-1-first drain pipe; 1-2-aseptic pipeline;
2-gas-liquid mixing pump; 2-1-second drain pipe; 3-knockout drum;
3-1-first escape pipe; 3-2-the 3rd drain pipe; 4-exhaust gas treating device;
5-ozone eliminator; 5-1-ultraviolet lamp; 5-2-strainer;
6-the first valve; 7-vacuum meter; 8-ozone concn detector;
9-the first tensimeter; 10-the second valve; 11-oxygenerator;
12-ozone machine; 12-1-second escape pipe; 13-oxygen flow meter;
14-the second tensimeter; 15-stopping valve; 16-fermentor tank.
Embodiment
Equipment of the present invention is described by embodiment 1:
Embodiment 1
As depicted in figs. 1 and 2 a kind of is applied to the equipment of microbial fermentation with ozone-containing sterilizing water, and this equipment comprises water treatment system and ozone unit; Described water treatment system comprises water tank 1, and the below of described water tank 1 is provided with and is connected with gas-liquid mixing pump 2 and is used for aseptic pipeline 1-2 that the water of water tank 1 is sent into the first drain pipe 1-1 in the gas-liquid mixing pump 2 and is connected with fermentor tank 16; The liquid outlet of described gas-liquid mixing pump 2 is provided with the second drain pipe 2-1 that is connected with knockout drum 3, the top of described knockout drum 3 is provided with the first escape pipe 3-1 that is connected with exhaust gas treating device 4 and is used for gas separated in the knockout drum 3 are sent into exhaust gas treating device 4, one side of described knockout drum 3 is provided with the 3rd drain pipe 3-2 that is connected with water tank 1 and is used for isolating liquid in the knockout drum 3 are sent into water tank 1, be provided with ozone eliminator 5 in the described first drain pipe 1-1, described the 3rd drain pipe 3-2 is provided with ozone concn detector 8, upward 2 direction is disposed with first valve 6 and vacuum meter 7 to the described first drain pipe 1-1 from ozone eliminator 5 to gas-liquid mixing pump, and upward 1 direction is disposed with first tensimeter 9 and second valve 10 to described the 3rd drain pipe 3-2 from ozone concn detector 8 to water tank;
Described ozone unit comprises oxygenerator 11, with the ozone machine 12 that is connected with oxygenerator 11 by pipeline, be disposed with the oxygen flow meter 13 and second tensimeter 14 on the pipeline between described oxygenerator 11 and the ozone machine 12, the air outlet of described ozone machine 12 is provided with the second escape pipe 12-1 that is connected with gas-liquid mixing pump 2 and is used for ozone is sent into gas-liquid mixing pump 2, and the described second escape pipe 12-1 is provided with stopping valve 15.
In the present embodiment, described ozone eliminator 5 is by being installed on the ultraviolet lamp 5-1 in the first drain pipe 1-1 and being installed in the first drain pipe 1-1 and the strainer 5-2 that is positioned at ultraviolet lamp 5-1 right side forms.
In the present embodiment, the wavelength of the emission light of described ultraviolet lamp 5-1 is 240nm～260nm.
In the present embodiment, described oxygenerator 11 is the PSA oxygen generator.
In the present embodiment, described gas-liquid mixing pump 2 is the Ni Keni pump.
In the present embodiment, described ozone machine 12 is the corona-type ozone machine.
Adopt equipment of the present invention to the microbial fermentation water carry out disinfection the sterilization method be described by embodiment 2 to embodiment 4:
Embodiment 2
The equipment that adopts embodiment 1 is to the sterilization that carries out disinfection of microbial fermentation water:
Step 1, close the aseptic pipeline 1-2 of water tank 1, close ozone eliminator 5, in water tank 1, inject 900L water, open gas-liquid mixing pump 2, water flows in the gas-liquid mixing pump 2 from water tank 1 by the first drain pipe 1-1, thereby and the opening degree of controlling first valve 6 velocity of flow of regulating water among the first drain pipe 1-1 to make the vacuum tightness of vacuum meter 7 be 0.005MPa, the volumetric flow rate that record water this moment is 30L/min;
Step 2, startup oxygenerator 11 system oxygen, regulate oxygen flow, the pressure for the treatment of second tensimeter 14 reaches 0.015MPa, keeps starting ozone machine 12 preparation ozone behind the 3min, opens stopping valve 15 simultaneously the ozone of preparation is entered in the gas-liquid mixing pump 2 by the second escape pipe 12-1;
Step 3, the ozone that enters gas-liquid mixing pump 2 in the water that flows into gas-liquid mixing pump 2 in the step 1 and the step 2 mixes in gas-liquid mixing pump 2, utilize ozone that water is carried out disinfection, mixed gas-liquid mixture enters and carries out gas-liquid separation in the knockout drum 3, open second valve 10, gas after the separation is discharged after exhaust gas treating device 4 is handled, water after the separation enters after ozone concn detector 8 detects finishes once circulation in the water tank 1, the flow that enters the ozone in the gas-liquid mixing pump 2 by stopping valve 15 controls makes the concentration of the ozone in water after separating maintain 2.5mg/L, and is keeping under the constant situation of this concentration water cycle being handled 30min; Time T=the V/Q of described circular treatment, wherein V is the volume (900L) of the water of injection water tank 1, Q is the volumetric flow rate (30L/min) of water;
Step 4, treat that circular treatment described in the step 3 finishes after, close stopping valve 15, close ozone machine 12 then, then close oxygenerator 11, open ozone eliminator 5, keep water in pipeline behind the circulation 20min ozone concn detector 8 detected ozone in water concentration be 0mg/L, open aseptic pipeline 1-2, close the first drain pipe 1-18 simultaneously, make the water after the circular treatment inject fermentor tank 9 as fermention medium constant volume water by aseptic pipeline 1-2.
Adopt the ozone-containing sterilizing water of present embodiment that sterilized basic medium in the fermentor tank is carried out constant volume, gauge pressure is the blank 48h of cultivation under the condition of 0.05MPa～0.06MPa in 37 ℃, jars, the 6h sampling of basic medium behind constant volume interval is checked, the result shows in each stage sample does not all have varied bacteria growing, illustrates that the ozone-containing sterilizing water of present embodiment can satisfy the requirement of microorganism pure-blood ferment.
Embodiment 3
The equipment that adopts embodiment 1 is to the sterilization that carries out disinfection of microbial fermentation water:
Step 1, close the aseptic pipeline 1-2 of water tank 1, close ozone eliminator 5, in water tank 1, inject 1000L water, open gas-liquid mixing pump 2, water flows in the gas-liquid mixing pump 2 from water tank 1 by the first drain pipe 1-1, thereby and the opening degree of controlling first valve 6 velocity of flow of regulating water among the first drain pipe 1-1 to make the vacuum tightness of vacuum meter 7 be 0.015MPa, the volumetric flow rate that record water this moment is 20L/min;
Step 2, startup oxygenerator 11 system oxygen, regulate oxygen flow, the pressure for the treatment of second tensimeter 14 reaches 0.030MPa, keeps starting ozone machine 12 preparation ozone behind the 5min, opens stopping valve 15 simultaneously the ozone of preparation is entered in the gas-liquid mixing pump 2 by the second escape pipe 12-1;
Step 3, the ozone that enters gas-liquid mixing pump 2 in the water that flows into gas-liquid mixing pump 2 in the step 1 and the step 2 mixes in gas-liquid mixing pump 2, utilize ozone that water is carried out disinfection, mixed gas-liquid mixture enters and carries out gas-liquid separation in the knockout drum 3, open second valve 10, gas after the separation is discharged after exhaust gas treating device 4 is handled, water after the separation enters after ozone concn detector 8 detects finishes once circulation in the water tank 1, the flow that enters the ozone in the gas-liquid mixing pump 2 by stopping valve 15 controls makes the concentration of the ozone in water after separating maintain 3.0mg/L, and is keeping under the constant situation of this concentration water cycle being handled 50min; Time T=the V/Q of described circular treatment, wherein V is the volume (1000L) of the water of injection water tank 1, Q is the volumetric flow rate (20L/min) of water;
Step 4, treat that circular treatment described in the step 3 finishes after, close stopping valve 15, close ozone machine 12 then, then close oxygenerator 11, open ozone eliminator 5, keep water in pipeline behind the circulation 40min ozone concn detector 8 detected ozone in water concentration be 0mg/L, open aseptic pipeline 1-2, close the first drain pipe 1-1 simultaneously, make the water after the circular treatment inject fermentor tank 9 as fermention medium constant volume water by aseptic pipeline 1-2.
Adopt the ozone-containing sterilizing water of present embodiment that sterilized basic medium in the fermentor tank is carried out constant volume, gauge pressure is the blank 48h of cultivation under the condition of 0.05MPa～0.06MPa in 37 ℃, jars, the 6h sampling of basic medium behind constant volume interval is checked, the result shows in each stage sample does not all have varied bacteria growing, illustrates that the ozone-containing sterilizing water of present embodiment can satisfy the requirement of microorganism pure-blood ferment.
Embodiment 4
The equipment that adopts embodiment 1 is to the sterilization that carries out disinfection of microbial fermentation water:
Step 1, close the aseptic pipeline 1-2 of water tank 1, close ozone eliminator 5, in water tank 1, inject 500L water, open gas-liquid mixing pump 2, water flows in the gas-liquid mixing pump 2 from water tank 1 by the first drain pipe 1-1, thereby and the opening degree of controlling first valve 6 velocity of flow of regulating water among the first drain pipe 1-1 to make the vacuum tightness of vacuum meter 7 be 0.010MPa, the volumetric flow rate that record water this moment is 25L/min;
Step 2, startup oxygenerator 11 system oxygen, regulate oxygen flow, the pressure for the treatment of second tensimeter 14 reaches 0.020MPa, keeps starting ozone machine 12 preparation ozone behind the 4min, opens stopping valve 15 simultaneously the ozone of preparation is entered in the gas-liquid mixing pump 2 by the second escape pipe 12-1;
Step 3, the ozone that enters gas-liquid mixing pump 2 in the water that flows into gas-liquid mixing pump 2 in the step 1 and the step 2 mixes in gas-liquid mixing pump 2, utilize ozone that water is carried out disinfection, mixed gas-liquid mixture enters and carries out gas-liquid separation in the knockout drum 3, open second valve 10, gas after the separation is discharged after exhaust gas treating device 4 is handled, water after the separation enters after ozone concn detector 8 detects finishes once circulation in the water tank 1, the flow that enters the ozone in the gas-liquid mixing pump 2 by stopping valve 15 controls makes the concentration of the ozone in water after separating maintain 2.8mg/L, and is keeping under the constant situation of this concentration water cycle being handled 20min; Time T=the V/Q of described circular treatment, wherein V is the volume (500L) of the water of injection water tank 1, Q is the volumetric flow rate (25L/min) of water;
Step 4, treat that circular treatment described in the step 3 finishes after, close stopping valve 15, close ozone machine 12 then, then close oxygenerator 11, open ozone eliminator 5, keep water in pipeline behind the circulation 10min ozone concn detector 8 detected ozone in water concentration be 0mg/L, open aseptic pipeline 1-2, close the first drain pipe 1-1 simultaneously, make the water after the circular treatment inject fermentor tank 9 as fermention medium constant volume water by aseptic pipeline 1-2.
Adopt the ozone-containing sterilizing water of present embodiment that sterilized basic medium in the fermentor tank is carried out constant volume, gauge pressure is the blank 48h of cultivation under the condition of 0.05MPa～0.06MPa in 37 ℃, jars, the 6h sampling of basic medium behind constant volume interval is checked, the result shows in each stage sample does not all have varied bacteria growing, illustrates that the ozone-containing sterilizing water of present embodiment can satisfy the requirement of microorganism pure-blood ferment.
The above; it only is preferred embodiment of the present invention; be not that the present invention is done any restriction, everyly any simple modification that above embodiment did, change and equivalent structure changed, all still belong in the protection domain of technical solution of the present invention according to the invention technical spirit.
Claims (7)
1. the sterilization device of a microbial fermentation water is characterized in that, this equipment comprises water treatment system and ozone unit; Described water treatment system comprises water tank (1), the below of described water tank (1) is provided with and is connected with gas-liquid mixing pump (2) and is used for aseptic pipeline (1-2) that the water of water tank (1) is sent into first drain pipe (1-1) in the gas-liquid mixing pump (2) and is connected with fermentor tank (16); The liquid outlet of described gas-liquid mixing pump (2) is provided with second drain pipe (2-1) that is connected with knockout drum (3), the top of described knockout drum (3) is provided with first escape pipe (3-1) that is connected with exhaust gas treating device (4) and is used for the interior gas separated of knockout drum (3) is sent into exhaust gas treating device (4), one side of described knockout drum (3) is provided with the 3rd drain pipe (3-2) that is connected with water tank (1) and is used for isolating liquid in the knockout drum (3) is sent into water tank (1), be provided with ozone eliminator (5) in described first drain pipe (1-1), described the 3rd drain pipe (3-2) is provided with ozone concn detector (8), the direction that described first drain pipe (1-1) is gone up from ozone eliminator (5) to gas-liquid mixing pump (2) is disposed with first valve (6) and vacuum meter (7), and the direction that described the 3rd drain pipe (3-2) is gone up from ozone concn detector (8) to water tank (1) is disposed with first tensimeter (9) and second valve (10);
Described ozone unit comprises oxygenerator (11), with the ozone machine (12) that is connected with oxygenerator (11) by pipeline, be disposed with oxygen flow meter (13) and second tensimeter (14) on the pipeline between described oxygenerator (11) and the ozone machine (12), the air outlet of described ozone machine (12) is provided with second escape pipe (12-1) that is connected with gas-liquid mixing pump (2) and is used for ozone is sent into gas-liquid mixing pump (2), and described second escape pipe (12-1) is provided with stopping valve (15).
2. the sterilization device of a kind of microbial fermentation water according to claim 1, it is characterized in that, described ozone eliminator (5) is by being installed on the ultraviolet lamp (5-1) in first drain pipe (1-1) and being installed in first drain pipe (1-1) and the strainer (5-2) that is positioned at ultraviolet lamp (5-1) right side is formed.
3. the sterilization device of a kind of microbial fermentation water according to claim 2 is characterized in that, the wavelength of the emission light of described ultraviolet lamp (5-1) is 240nm～260nm.
4. the sterilization device of a kind of microbial fermentation water according to claim 1 is characterized in that, described oxygenerator (11) is the PSA oxygen generator.
5. the sterilization device of a kind of microbial fermentation water according to claim 1 is characterized in that, described gas-liquid mixing pump (2) is the Ni Keni pump.
6. the sterilization device of a kind of microbial fermentation water according to claim 1 is characterized in that, described ozone machine (12) is the corona-type ozone machine.
7. one kind is utilized equipment according to claim 1 to the carry out disinfection method of sterilization of microbial fermentation water, it is characterized in that this method may further comprise the steps:
Step 1, close the aseptic pipeline (1-2) of water tank (1), close ozone eliminator (5), in water tank (1), inject water, open gas-liquid mixing pump (2), water flows in the gas-liquid mixing pump (2) from water tank (1) by first drain pipe (1-1), and control first valve (6) thus the opening degree velocity of flow of regulating the middle water of first drain pipe (1-1) to make the vacuum tightness of vacuum meter (7) be 0.005MPa～0.015MPa;
Step 2, startup oxygenerator (11) system oxygen, regulate oxygen flow, the pressure for the treatment of second tensimeter (14) reaches 0.015MPa～0.030MPa, keep starting ozone machine (12) preparation ozone behind 3min～5min, open stopping valve (15) simultaneously the ozone of preparation is entered in the gas-liquid mixing pump (2) by second escape pipe (12-1);
Step 3, the ozone that enters gas-liquid mixing pump (2) in the water that flows into gas-liquid mixing pump (2) in the step 1 and the step 2 mixes in gas-liquid mixing pump (2), utilize ozone that water is carried out disinfection, mixed gas-liquid mixture enters in the knockout drum (3) and carries out gas-liquid separation, open second valve (10), gas after the separation is discharged after exhaust gas treating device (4) is handled, water after the separation enters after ozone concn detector (8) detects and finishes once circulation in the water tank (1), the flow that enters the ozone in the gas-liquid mixing pump (2) by stopping valve (15) control makes the concentration of the ozone in water after separating maintain 2.5mg/L～3.0mg/L, and is keeping under the constant situation of this concentration water being carried out circular treatment; Time T=the V/Q of described circular treatment, wherein V is the volume of the water of injection water tank (1), Q is the volumetric flow rate of water;
Step 4, treat that circular treatment described in the step 3 finishes after, close stopping valve (15), close ozone machine (12) then, then close oxygenerator (11), open ozone eliminator (5), when keeping water in pipeline, to be circulated to the detected ozone in water concentration of ozone concn detector (8) being 0mg/L, open aseptic pipeline (1-2), close first drain pipe (1-1) simultaneously, make the water after the circular treatment inject fermentor tank (9) as fermention medium constant volume water by aseptic pipeline (1-2).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111470611A (en) * | 2020-04-21 | 2020-07-31 | 安徽省金裕皖酒业有限公司 | Sterilization equipment for water for microbial fermentation and use method thereof |
| CN111849737A (en) * | 2020-06-10 | 2020-10-30 | 北京首钢朗泽新能源科技有限公司 | Fermentation system and method |
| CN112695067A (en) * | 2021-01-27 | 2021-04-23 | 泗阳华盛生物科技有限公司 | Tapping sampling and detection method for liquid fermentation tank strain |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2618917Y (en) * | 2003-02-18 | 2004-06-02 | 广东美的集团股份有限公司 | Device capable of purifying and sterilizing water and air |
| CN101803866A (en) * | 2009-02-17 | 2010-08-18 | 罗莎国际有限公司 | High-dissolved oxygen raw water dispenser |
| CN102659275A (en) * | 2012-05-21 | 2012-09-12 | 杭州美亚水处理科技有限公司 | Method and device for keeping drinking water in water storage tank fresh |
-
2013
- 2013-05-19 CN CN2013101855587A patent/CN103224280A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2618917Y (en) * | 2003-02-18 | 2004-06-02 | 广东美的集团股份有限公司 | Device capable of purifying and sterilizing water and air |
| CN101803866A (en) * | 2009-02-17 | 2010-08-18 | 罗莎国际有限公司 | High-dissolved oxygen raw water dispenser |
| CN102659275A (en) * | 2012-05-21 | 2012-09-12 | 杭州美亚水处理科技有限公司 | Method and device for keeping drinking water in water storage tank fresh |
Non-Patent Citations (1)
| Title |
|---|
| 张晖: "紫外光辐射下臭氧在水中的分解动力学", 《高校化学工程学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111470611A (en) * | 2020-04-21 | 2020-07-31 | 安徽省金裕皖酒业有限公司 | Sterilization equipment for water for microbial fermentation and use method thereof |
| CN111470611B (en) * | 2020-04-21 | 2022-12-06 | 安徽省金裕皖酒业有限公司 | Sterilization equipment for microbial fermentation water and use method thereof |
| CN111849737A (en) * | 2020-06-10 | 2020-10-30 | 北京首钢朗泽新能源科技有限公司 | Fermentation system and method |
| CN111849737B (en) * | 2020-06-10 | 2021-05-04 | 北京首钢朗泽新能源科技有限公司 | Fermentation system and method |
| CN112695067A (en) * | 2021-01-27 | 2021-04-23 | 泗阳华盛生物科技有限公司 | Tapping sampling and detection method for liquid fermentation tank strain |
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Application publication date: 20130731 |