CN103215332A - Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein - Google Patents
Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein Download PDFInfo
- Publication number
- CN103215332A CN103215332A CN2013101323354A CN201310132335A CN103215332A CN 103215332 A CN103215332 A CN 103215332A CN 2013101323354 A CN2013101323354 A CN 2013101323354A CN 201310132335 A CN201310132335 A CN 201310132335A CN 103215332 A CN103215332 A CN 103215332A
- Authority
- CN
- China
- Prior art keywords
- casein
- solution
- ace
- make
- water bath
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein. The method comprises the following steps of: cooling and centrifuging recovered feta for degreasing to obtain skimmed milk, using the skimmed mild for preparing casein by isoelectric precipitation, firstly hydrolyzing the casein with Alcalase to obtain hydrolysate containing the ACE inhibitory peptides through optimization of hydrolysis condition, and performing optimization of neutral protease and trypsin hydrolysis conditions by taking the hydrolysate as a substrate to obtain small-molecule peptides with high ACE inhibitory activity through. The inhibitory proportion of an ACE inhibitory peptide product prepared by the method can reach above 90%; and the ACE inhibitory peptide product has the characteristics of being diversified in variety of the ACE inhibitory peptides, having small fragments, being beneficial to the separation and purification of the small-molecule peptides and being high in inhibitory proportion.
Description
Technical field
The present invention relates to a kind of method of substep enzymolysis fetta cheese protein Preparation ace inhibitory peptide.
Background technology
Hypertension is one of modal cardiovascular disorder, often causes the heart, brain, kidney complication, is the primary hazard factor of cerebral apoplexy coronary heart disease.Hypertension is meant not to be used under the situation of antihypertensive drugs, systolic pressure 〉=140mmHg and (or) situation of diastolic pressure 〉=90mmHg.According to national hypertension research centre recent statistics, China hyperpietic has reached 1.2 hundred million people, and sickness rate is 10.8%, and its hazard rating is only second to tumour, has become No. second killer of harm humans health.According to World Health Organization's prediction, to the year two thousand twenty, Non Communicable Diseases (NCD) will account for 79% of China's cause of death, and wherein cardiovascular diseases will account for the first place.Every reduction 5mmHg systolic pressure is suffered from cardiovascular disease risk and is just reduced by 16%.
At present, being usually used in the 6 kinds of antihypertensive drugs that mainly contain of antihypertensive drug treatment, is respectively diuretic(s), alpha-receptor antagonist, calcium channel blocker, angiotensin-convertion enzyme inhibitor, Angiotensin and alpha-2-adrenoceptor retarding agent.Though these Altace Ramiprils have significant antihypertensive effect, there is certain toxic side effect, increase the weight of as cough, supersensitivity angioedema and renal insufficiency etc.
Zinc metallopeptidase Zace1 (angiotensin – eonverting enzyme, ACE) in blood pressure regulation, play an important role, excision through two amino acid of carbon teminal (His-Leu), can change the angiotensin I of script non-activity into and have active Angiotensin II, cause vasoconstriction, make increased blood pressure; ACE also can make has the sharp skin inactivation of releiving of vasorelaxation function, causes the situation of increased blood pressure equally again, and ACE suppresses skin can block two kinds of biochemical reaction processes that ACE causes, plays hypotensive effect.
The preparation method of ACE mainly contains 5 kinds: chemical degradation method, enzymolysis process, microbe fermentation method, chemical synthesis and gene engineering research, studying maximum now is enzymolysis process and microbe fermentation method.With respect to microbe fermentation method, the enzyme process reaction prepares ace inhibitory peptide and suppresses active high fast, and raw material is extensive, mainly contain lactoprotein, plant proteinoid and animal proteinoid, but present newborn source ACE inhibitor peptides mainly is to be raw material with cow's milk, and the sheep breast is not related to as yet.
Summary of the invention
In order to overcome the shortcoming of above-mentioned prior art, the object of the present invention is to provide a kind of method of substep enzymolysis fetta cheese protein Preparation ace inhibitory peptide, casein is through ultrasonication, and structure changes, method by complex enzyme hydrolysis makes hydrolysis reaction more thorough again, and is rapider.
In order to achieve the above object, the technical scheme taked of the present invention is:
A kind of method of the enzymolysis fetta cheese protein Preparation ace inhibitory peptide that distributes may further comprise the steps:
Step 1: will restore the sheep breast and be cooled to 4 ℃, and use supercentrifuge under 6400rpm, behind the centrifugal 15min, to remove fat, and obtain skimming milk;
Step 2: will be incubated in the constant water bath box of gained skimming milk in 35-75 ℃ of scope, adopt the magnetic force heating stirrer to stir, stirring velocity is 0-1600rpm, drip HCl solution while stirring, make the pH value of skimming milk solution be 4.2-5.0, re-using supercentrifuge centrifugal 15min under 6400rpm precipitates casein, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of following pre-freeze 12h, transfer to freeze drier again at-55 ℃ of following lyophilize 24h, obtain the casein lyophilized powder, measure the casein yield;
Step 3: gained casein lyophilized powder is mixed with pure water, make mass concentration and be 10% casein solution, with the ultrasonic pretreatment 60min of 180w;
Step 4: from the gained casein solution, take out 100ml, in 45-65 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of casein solution be 6.5-8.5; According to the enzyme-to-substrate mass ratio is the ratio of 2-10:100, adds alkaline enzyme Alcalase, during hydrolysis 1-3h; In the hydrolytic process, every interval 0.5h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm then, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active;
Step 5: the gained supernatant liquor is drawn 50ml, in 35-55 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of this solution be 7.0-9.0, add neutral protease and trypsinase that mass ratio is 1:1,1:2,2:1,4:1 more respectively, used enzyme-to-substrate mass ratio is 2-10:100, hydrolysis 5h; In the hydrolytic process, every interval 1h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active, and supernatant liquor carried out lyophilize, obtain ace inhibitory peptide.
Described recovery sheep breast carries out the resulting solution of mixed dissolution for goat milk powder and pure water according to mass ratio 1:9.
Compared with prior art, beneficial effect of the present invention is:
The present invention adopts the method for distribution enzymolysis that casein is hydrolyzed, at first the Alcalase enzyme acts on the casein that ultrasonication is crossed, obtain containing the hydrolyzed solution of high reactivity ace inhibitory peptide, to hydrolyzed solution hydrolysis once more, make the not hydrolysis thorough hydrolysis of casein completely with neutral protease and trypsinase.Different enzymes act on different positions in different time sections to casein, and the ace inhibitory peptide kind of generation is more, and fragment is littler, helps the separation and purification of small-molecular peptides.
Its inhibition ratio of ace inhibitory peptide product of the present invention's preparation can reach more than 90%.It is added in the varieties of food items as functional factor, the hyperpietic is had hypotensive effect, and character is gentle and normotensive people is not had effect, it also has immunocompetence, promoting digestion absorbs and have multiple efficacies such as fat-reducing.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
A kind of method of the enzymolysis fetta cheese protein Preparation ace inhibitory peptide that distributes may further comprise the steps:
Step 1: will restore the sheep breast and be cooled to 4 ℃, and use supercentrifuge under 6400rpm, behind the centrifugal 15min, to remove fat, and obtain skimming milk;
Step 2: will be incubated in the constant water bath box of gained skimming milk in 35-75 ℃ of scope, adopt the magnetic force heating stirrer to stir, stirring velocity is 0-1600rpm, drip HCl solution while stirring, make the pH value of skimming milk solution be 4.2-5.0, use supercentrifuge centrifugal 15min under 6400rpm that casein is precipitated, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of pre-freeze 12h, transfer to freeze drier again at-55 ℃ of following lyophilize 24h, obtain the casein lyophilized powder, measure the casein yield; Described skimming milk is 45 ℃ in temperature, and the pH value is 4.5, and stirring intensity is under the condition of 800rpm, and casein yield maximum reaches 11.88%.
Step 3: gained casein lyophilized powder is mixed with pure water, make mass concentration and be 10% casein solution, with the ultrasonic pretreatment 60min of 180w;
Step 4: from the gained casein solution, take out 100ml, in 45-65 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of casein solution be 6.5-8.5; According to the enzyme-to-substrate mass ratio is that (enzyme dosage [E]/[S]=2%-10%) adds alkaline enzyme Alcalase, during hydrolysis 1-3h for the ratio of 2-10:100; In the hydrolytic process, every interval 0.5h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm then, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active; At the Alcalase enzyme dosage is [E]/[S]=6%, and temperature is that 50 ℃, pH value are 8.0, under the condition of hydrolysis time 2.5h, and it is maximum that the ACE inhibiting rate reaches, and is 88.32%.
Step 5: the gained supernatant liquor is drawn 50ml, in 35-55 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of this solution be 7.0-9.0, add neutral protease and trypsinase that mass ratio is 1:1,1:2,2:1,4:1 respectively, used enzyme-to-substrate mass ratio is the total consumption of 2-10:100(enzyme [E]/[S]=2%-10%), hydrolysis 5h; In the hydrolytic process, every interval 1h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active, and supernatant liquor carried out lyophilize, obtain ace inhibitory peptide.At neutral protease and tryptic mass ratio is that 1:1, total consumption be [E]/[S]=4%, and temperature is that 40 ℃, pH value are 7.5, and hydrolysis time is under the condition of 1h, and the ACE inhibiting rate reaches maximum, is 92.14%.
Described recovery sheep breast carries out the resulting solution of mixed dissolution for goat milk powder and pure water according to mass ratio 1:9.
Embodiment one
Step 1: goat milk powder and water are carried out mixed dissolution according to mass ratio 1:9, and the recovery sheep breast that obtains is cooled to 4 ℃, uses supercentrifuge behind the centrifugal 15min, to remove fat under 6400rpm, obtains skimming milk;
Step 2: the gained skimming milk is incubated in 35 ℃ constant water bath box, adopt the magnetic force heating stirrer to stir, stirring velocity is 800rpm, drip HCl solution while stirring, the pH value that makes skimming milk solution is 4.6, casein precipitates, use supercentrifuge under 6400rpm behind the centrifugal 15min, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of pre-freeze 12h, transfers to freeze drier again at-55 ℃ of following lyophilize 24h, obtains the casein lyophilized powder; When skimming milk is 35 ℃ in temperature, the pH value is 4.6, and stirring intensity is under the 800rpm condition, and casein yield maximum reaches 9.23%.
Embodiment two
Step 1: goat milk powder and water are carried out mixed dissolution according to mass ratio 1:9, and the recovery sheep breast that obtains is cooled to 4 ℃, uses supercentrifuge behind the centrifugal 15min, to remove fat under 6400rpm, obtains skimming milk;
Step 2: will be incubated in the constant water bath box of gained skimming milk in 45 ℃ of scopes, adopt the magnetic force heating stirrer to stir, stirring velocity is 800rpm, drip HCl solution while stirring, make the PH of skimming milk solution reach 4.5, casein is precipitated, use supercentrifuge under 6400rpm behind the centrifugal 15min, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of pre-freeze 12h, transfers to freeze drier again at-55 ℃ of following lyophilize 24h, obtains the casein lyophilized powder; When skimming milk at 45 ℃, pH is 4.5, stirring intensity is under the 800rpm condition, casein yield maximum reaches 11.88%.
Step 3: gained casein lyophilized powder is mixed with pure water, make mass concentration and be 10% casein solution, with the ultrasonic pretreatment 60min of 180w;
Step 4: take out 100ml from the gained casein solution, be incubated in 45 ℃ constant water bath box, splash into the NaOH solution of 1mol/l, the pH value that makes casein solution is 6.5; According to the enzyme-to-substrate mass ratio is the ratio (enzyme dosage [E]/[S]=4%) of 4:100, adds alkaline enzyme Alcalase, during hydrolysis 2h; Get the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm then, remove unhydrolysed casein, collecting supernatant liquor and adopting determined by ultraviolet spectrophotometry ACE to suppress activity is 82.35%.
Embodiment three
Step 1: goat milk powder and water are carried out mixed dissolution according to mass ratio 1:9, obtain recovery sheep breast be cooled to 4 ℃, use supercentrifuge under 6400rpm, behind the centrifugal 15min, to remove fat, obtain skimming milk;
Step 2: the gained skimming milk is incubated in 45 ℃ constant water bath box, adopt the magnetic force heating stirrer to stir, stirring velocity is 800rpm, drip HCl solution while stirring, the pH value that makes skimming milk solution is 4.5, casein precipitates, use supercentrifuge under 6400rpm behind the centrifugal 15min, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of pre-freeze 12h, transfers to freeze drier again at-55 ℃ of following lyophilize 24h, obtains the casein lyophilized powder; When skimming milk is 45 ℃ in temperature, the pH value is 4.5, and stirring intensity is under the 800rpm condition, and casein yield maximum reaches 11.88%.
Step 3: gained casein lyophilized powder is mixed with pure water, make mass concentration and be 10% casein solution, with the ultrasonic pretreatment 60min of 180w;
Step 4: take out 100ml from the gained casein solution, be incubated in 45-65 ℃ constant water bath box, splash into the NaOH solution of 1mol/l, the pH value that makes casein solution is 8.0; According to the enzyme-to-substrate mass ratio is the ratio (enzyme dosage [E]/[S]=6%) of 6:100, adds alkaline enzyme Alcalase, during hydrolysis 2.5h; Get the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm then, remove unhydrolysed casein, collecting supernatant liquor and adopting determined by ultraviolet spectrophotometry ACE to suppress activity is 88.32%.
Step 5: the gained supernatant liquor is drawn 50ml, in 40 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, the pH value that makes this solution is 7.5, adding mass ratio is neutral protease and the trypsinase of 1:1, used enzyme-to-substrate mass ratio is 4:100(enzyme dosage [E]/[S]=4%), hydrolysis 1h; Get the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm, remove unhydrolysed casein, collecting supernatant liquor and adopting determined by ultraviolet spectrophotometry ACE to suppress activity is 92.14%.
Above-mentioned specific embodiment has further been set forth the present invention.Should be understood that these examples only to be used to the present invention is described and be not used in and limit the scope of the invention.
Claims (2)
1. method of enzymolysis fetta cheese protein Preparation ace inhibitory peptide that distributes is characterized in that: may further comprise the steps:
Step 1: will restore the sheep breast and be cooled to 4 ℃, and use supercentrifuge under 6400rpm, behind the centrifugal 15min, to remove fat, and obtain skimming milk;
Step 2: will be incubated in the constant water bath box of gained skimming milk in 35-75 ℃ of scope, adopt the magnetic force heating stirrer to stir, stirring velocity is 0-1600rpm, drip HCl solution while stirring, make the pH value of skimming milk solution be 4.2-5.0, use supercentrifuge centrifugal 15min under 6400rpm that casein is precipitated, abandoning supernatant, sedimentary casein is carried out lyophilize, in temperature is-40 ℃ of pre-freeze 12h, transfer to freeze drier again at-55 ℃ of following lyophilize 24h, obtain the casein lyophilized powder, measure the casein yield;
Step 3: gained casein lyophilized powder is mixed with pure water, make mass concentration and be 10% casein solution, with the ultrasonic pretreatment 60min of 180w.
Step 4: from the gained casein solution, take out 100ml, in 45-65 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of casein solution be 6.5-8.5; According to the enzyme-to-substrate mass ratio is the ratio of 2-10:100, adds alkaline enzyme Alcalase, during hydrolysis 1-3h; In the hydrolytic process, every interval 0.5h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm then, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active;
Step 5: the gained supernatant liquor is drawn 50ml, in 35-55 ℃ constant water bath box, be incubated, splash into the NaOH solution of 1mol/l, make the pH value of this solution be 7.0-9.0, add neutral protease and trypsinase that mass ratio is 1:1,1:2,2:1,4:1 respectively, used enzyme-to-substrate mass ratio is 2-10:100, hydrolysis 5h; In the hydrolytic process, every interval 1h gets the 5ml hydrolyzed solution, in 90 ℃ constant water bath box, keep 10min, make alkaline enzyme Alcalase inactivation, use supercentrifuge centrifugal 20min under the condition of 9000rpm, remove unhydrolysed casein, collect supernatant liquor and adopt determined by ultraviolet spectrophotometry ACE to suppress active, and supernatant liquor carried out lyophilize, obtain ace inhibitory peptide.
2. the method for a kind of enzymolysis fetta cheese protein Preparation ace inhibitory peptide that distributes according to claim 1 is characterized in that: described recovery sheep breast carries out the resulting solution of mixed dissolution for goat milk powder and pure water according to mass ratio 1:9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101323354A CN103215332A (en) | 2013-04-16 | 2013-04-16 | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101323354A CN103215332A (en) | 2013-04-16 | 2013-04-16 | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103215332A true CN103215332A (en) | 2013-07-24 |
Family
ID=48813482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101323354A Pending CN103215332A (en) | 2013-04-16 | 2013-04-16 | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103215332A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031965A (en) * | 2014-06-05 | 2014-09-10 | 西北农林科技大学 | Preparation method of goat cheese protein source antibacterial peptide |
| CN107964034A (en) * | 2017-11-13 | 2018-04-27 | 江苏大学 | The ultrasonic wave added simulation digestion method of casein active peptide and health food application |
| CN110128523A (en) * | 2019-06-04 | 2019-08-16 | 海普诺凯营养品有限公司 | One kind, which has, adjusts active feta proteolysis peptide of bone-marrow-derived lymphocyte and preparation method thereof |
| CN110128522A (en) * | 2019-06-04 | 2019-08-16 | 海普诺凯营养品有限公司 | One kind, which has, adjusts active feta proteolysis peptide of bone-marrow-derived lymphocyte and preparation method thereof |
| CN114736268A (en) * | 2022-03-01 | 2022-07-12 | 吉林农业大学 | Modification method for improving ACE inhibition rate of active peptide, ACE inhibition peptide and application of ACE inhibition peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1231279A1 (en) * | 1999-11-11 | 2002-08-14 | Calpis Co., Ltd. | Process for producing tripeptides |
| CN1552891A (en) * | 2003-11-10 | 2004-12-08 | 东北农业大学 | Preparation of dairy ACE inhibitory peptide |
| CN1833031A (en) * | 2003-08-01 | 2006-09-13 | 卡尔皮斯株式会社 | Casein hydrolyzate, process for producing the same and use thereof |
-
2013
- 2013-04-16 CN CN2013101323354A patent/CN103215332A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1231279A1 (en) * | 1999-11-11 | 2002-08-14 | Calpis Co., Ltd. | Process for producing tripeptides |
| CN1833031A (en) * | 2003-08-01 | 2006-09-13 | 卡尔皮斯株式会社 | Casein hydrolyzate, process for producing the same and use thereof |
| CN1552891A (en) * | 2003-11-10 | 2004-12-08 | 东北农业大学 | Preparation of dairy ACE inhibitory peptide |
Non-Patent Citations (1)
| Title |
|---|
| 姜瞻梅等: "酶解牛乳酪蛋白制备ACE抑制肽的研究", 《中国食品学报》, vol. 7, no. 6, 31 December 2007 (2007-12-31), pages 39 - 43 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031965A (en) * | 2014-06-05 | 2014-09-10 | 西北农林科技大学 | Preparation method of goat cheese protein source antibacterial peptide |
| CN107964034A (en) * | 2017-11-13 | 2018-04-27 | 江苏大学 | The ultrasonic wave added simulation digestion method of casein active peptide and health food application |
| CN110128523A (en) * | 2019-06-04 | 2019-08-16 | 海普诺凯营养品有限公司 | One kind, which has, adjusts active feta proteolysis peptide of bone-marrow-derived lymphocyte and preparation method thereof |
| CN110128522A (en) * | 2019-06-04 | 2019-08-16 | 海普诺凯营养品有限公司 | One kind, which has, adjusts active feta proteolysis peptide of bone-marrow-derived lymphocyte and preparation method thereof |
| CN114736268A (en) * | 2022-03-01 | 2022-07-12 | 吉林农业大学 | Modification method for improving ACE inhibition rate of active peptide, ACE inhibition peptide and application of ACE inhibition peptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103215332A (en) | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein | |
| CN101701240B (en) | Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein | |
| CN103215331A (en) | Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through compound enzymatic hydrolysis of feta protein | |
| CN102550797B (en) | Production method of sea cucumber peptide extract | |
| CN101514355B (en) | Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling | |
| CN105219826A (en) | A kind of have oyster peptide of enhancing function and its preparation method and application | |
| CN103215333A (en) | Method for preparing angiotensin-I-converting enzyme (ACE) inhibitory peptide by using enzymolysis goat milk casein | |
| CN101979532B (en) | Method for comprehensively using pig blood | |
| CN103211024A (en) | Method for preparing goat milk beverage containing ACE (Angiotensin Converting Enzyme) inhibitory peptide | |
| CN104774896A (en) | Preparation method for iron-chelated collagen peptide of hairtail fish-bones | |
| CN102058129A (en) | Method for preparing walnut polypeptide beverage | |
| CN104131057A (en) | Functional white kidney bean polypeptide and preparation method and application thereof | |
| CN102296100A (en) | Preparation method of casein antihypertensive peptides | |
| CN113088548A (en) | Preparation method of oyster antioxidant active peptide | |
| CN111184219A (en) | Method for preparing polypeptide compound by combining tea polyphenol and polypeptide | |
| CN102286590A (en) | Preparation method for jelly fish neurotensin | |
| CN109957595A (en) | A kind of preparation process of pork liver Gly-His-Lys | |
| CN103229831A (en) | Preparation method of high-calcium goat yogurt fermented by lactobacillus plantarum containing ACE (Angiotensin Converting Enzyme) inhibitory peptides | |
| CN103222537B (en) | Method for preparing peanut peptides through step enzymatic hydrolysis of peanut protein isolate by using two neutral proteases | |
| CN103444979A (en) | Method for lowering albumen allergenicity by immobilized enzyme hydrolysis | |
| CN104450845A (en) | Method for preparing sea cucumber polypeptide from sea cucumber endogenous protease | |
| CN101353687A (en) | Melissa powder peptides having angiotensin transferase inhibitory activity and preparation thereof | |
| CN103478739B (en) | A kind of high-quality garlic skin dietary fiber and preparation method thereof | |
| CN103266156A (en) | Method for preparing deer blood protein peptide by using fractional enzymolysis technology | |
| CN103222505A (en) | Preparation method of lactobacillus plantarum fermented goat milk containing ACE inhibitory peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130724 |