CN103207175A - Free fatty acid determination reagent kit - Google Patents
Free fatty acid determination reagent kit Download PDFInfo
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- CN103207175A CN103207175A CN 201310085639 CN201310085639A CN103207175A CN 103207175 A CN103207175 A CN 103207175A CN 201310085639 CN201310085639 CN 201310085639 CN 201310085639 A CN201310085639 A CN 201310085639A CN 103207175 A CN103207175 A CN 103207175A
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- China
- Prior art keywords
- fatty acid
- reagent
- free fatty
- acetylcoenzyme
- reagent kit
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Abstract
The invention relates to a free fatty acid determination reagent kit which comprises dual reagents. The reagent 1 comprises Good's buffer solution (pH(potential of hydrogen)=7.0), coenzyme A, ATP (adenosine triphosphate), acetylcoenzyme A synthetase, magnesium chloride (MgCL2), Trinder bound constituent, tween 80 and liquid biological preservative/Proclin 300, and the reagent 2 comprises Good's buffer solution (pH=7.0), acetylcoenzyme A oxidase (ACOD), peroxidase (POD), Trinder bound constituent, tween 80 and liquid biological preservative/Proclin 300. The free fatty acid determination reagent kit has the remarkable advantages that the reagent kit is directly used for an automatic biochemical analyzer and is simple; and the adopted automatically separated and purified acetylcoenzyme A synthetase and acetylcoenzyme A oxidase (ACOD) have high specificity on free fatty acid in serum.
Description
Technical field
The invention belongs to medical 6840 external diagnosis reagent fields, be specifically related to a kind of free-fat acidity test kit.
Background technology
Free fatty acid also is non-esterified fatty acid, is the hydrolysate of body fat mass in human body.Free fatty acid raises and is found in some pathological states such as insulin resistance/diabetes B, obesity, malignant disease and metabolic syndrome, and can cause angiocardiopathy.The content of measuring free fatty acid becomes most important, and the mensuration aspect deviation on the market is too many before, not too science.
Summary of the invention
The object of the present invention is to provide a kind of composition simpler, easy to use, can detect the mensuration kit of free fatty acid fast and accurately.
The present invention is achieved by the following technical programs:
The invention provides a kind of free-fat acidity test kit, this kit is made of following double reagent, wherein
Reagent 1:
Reagent 2:
The inspection principle of free-fat acidity test of the present invention is that free fatty acid and coacetylase react the generation acetyl coenzyme A under the effect of acetyl-CoA-synthetase (ACS), acetyl coenzyme A becomes to give birth to H2O2 under the oxidasic effect of acetyl coenzyme A, generate coloured product by the Trinder substrate under the effect of peroxidase (POD) subsequently.
Damping fluid is Good ' s damping fluid (pH=7.0) in mentioned reagent 1 of the present invention and the reagent 2.
Another technical matters to be solved by this invention is to provide the present invention to be used for the method for clinical examination free serum fatty acid.
This method relies on above-mentioned enzyme process principle, specifically may further comprise the steps:
(1) condition determination
37 ℃ of temperature; Predominant wavelength 546nm (biochemical instruments of different model can be selected by nearby principle); Method: end-point method, the reagent sample ratio: reagent 1: reagent 2: S is 210: 70: 4
(2) operation steps
(3) calibration procedure
Operate according to the calibration object operation instructions: calibration object adopts has registration certificate or the qualified calibration object (the Landau calibration object is used in suggestion) through verifying.Calibration frequency: after 1, the reagent lot number is changed; 2, according to the Quality Control requirement.
(3) Quality Control Procedure
Operate according to the quality-control product operation instructions: quality-control product adopts Landau or Luo Shi etc. to have the quality-control product of registration certificate.Advise carrying out every day Quality Control experiment.
(4) calculate
NEFA(mmol/L)＝(ΔA
T)/(ΔA
s)×C
S
Δ A
T: measure the blank relatively pipe absorbance of pipe
Δ A
S: the blank relatively pipe absorbance of calibration tube
C
S: calibration object concentration
Compared with prior art, free-fat acidity test kit of the present invention has following remarkable advantage and beneficial effect:
(1) kit is directly used in full automatic biochemical apparatus, and is simple.
(2) acetyl-CoA-synthetase and the acetyl coenzyme A oxidase (ACOD) of the own separation and purification of employing have high specificity to the free fatty acid in the serum.
Embodiment
Describe in further detail below in conjunction with the present invention of embodiment.
Embodiment
Be used for clinical detection free serum fatty acid and measure kit, constituted by following double reagent by this kit, wherein
Reagent 1:
Reagent 2:
Measure sample, adopt 2 end-point methods, 37 ℃ of temperature; Predominant wavelength 546nm (biochemical instruments of different model can be selected by nearby principle); Method: end-point method, the reagent sample ratio: reagent 1: reagent 2: S is 210: 70: 4
Free fatty acid (mmol/L)=concentration of standard solution (mmol/L) * Δ A sample/Δ A titer.
Embodiments of the invention are can not be used for restriction the present invention to explanation of the present invention, and the implication suitable with claims of the present invention and any change in the scope all should be thought to comprise in the scope of claims.
Claims (2)
1. free-fat acidity test kit, this kit is made of following double reagent, wherein
Reagent 1:
Reagent 2:
2. described for clinical free-fat acidity test kit according to claims 1, it is characterized in that: the damping fluid in described reagent 1 and the reagent 2 is Good ' s damping fluid (pH=7.0) 50mmol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310085639 CN103207175A (en) | 2013-03-15 | 2013-03-15 | Free fatty acid determination reagent kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310085639 CN103207175A (en) | 2013-03-15 | 2013-03-15 | Free fatty acid determination reagent kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103207175A true CN103207175A (en) | 2013-07-17 |
Family
ID=48754463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201310085639 Pending CN103207175A (en) | 2013-03-15 | 2013-03-15 | Free fatty acid determination reagent kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103207175A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983667A (en) * | 2014-04-24 | 2014-08-13 | 南京财经大学 | Apparatus and method for rapidly determining free fatty acid |
| CN104480077A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Recombination acetyl coenzyme A synthetase |
| CN105203472A (en) * | 2015-06-30 | 2015-12-30 | 北京万泰德瑞诊断技术有限公司 | Stable NEFA (non-esterified fatty acid) measuring kit |
| CN106191213A (en) * | 2016-09-19 | 2016-12-07 | 中拓生物有限公司 | A kind of free fatty acid determination reagent kit and preparation method thereof |
| CN106442496A (en) * | 2016-09-20 | 2017-02-22 | 上海科华生物工程股份有限公司 | Free fatty acid detection kit |
| CN113418877A (en) * | 2021-06-09 | 2021-09-21 | 东软威特曼生物科技(南京)有限公司 | Free fatty acid detection kit and method |
-
2013
- 2013-03-15 CN CN 201310085639 patent/CN103207175A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983667A (en) * | 2014-04-24 | 2014-08-13 | 南京财经大学 | Apparatus and method for rapidly determining free fatty acid |
| CN103983667B (en) * | 2014-04-24 | 2016-04-13 | 南京财经大学 | A kind of free fatty acid rapid determination device and detection method |
| CN104480077A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Recombination acetyl coenzyme A synthetase |
| CN104480077B (en) * | 2014-12-22 | 2017-03-01 | 宁波美康生物科技股份有限公司 | Restructuring acetyl-CoA-synthetase |
| CN105203472A (en) * | 2015-06-30 | 2015-12-30 | 北京万泰德瑞诊断技术有限公司 | Stable NEFA (non-esterified fatty acid) measuring kit |
| CN105203472B (en) * | 2015-06-30 | 2019-03-08 | 北京万泰德瑞诊断技术有限公司 | A kind of stable free fatty acid determination reagent kit |
| CN106191213A (en) * | 2016-09-19 | 2016-12-07 | 中拓生物有限公司 | A kind of free fatty acid determination reagent kit and preparation method thereof |
| CN106191213B (en) * | 2016-09-19 | 2019-10-11 | 中拓生物有限公司 | A kind of free fatty acid determination reagent kit and preparation method thereof |
| CN106442496A (en) * | 2016-09-20 | 2017-02-22 | 上海科华生物工程股份有限公司 | Free fatty acid detection kit |
| CN113418877A (en) * | 2021-06-09 | 2021-09-21 | 东软威特曼生物科技(南京)有限公司 | Free fatty acid detection kit and method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130717 |