CN103207149A - Infrared gel positioning device and gel imaging system utilizing same - Google Patents
Infrared gel positioning device and gel imaging system utilizing same Download PDFInfo
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- CN103207149A CN103207149A CN2013101333479A CN201310133347A CN103207149A CN 103207149 A CN103207149 A CN 103207149A CN 2013101333479 A CN2013101333479 A CN 2013101333479A CN 201310133347 A CN201310133347 A CN 201310133347A CN 103207149 A CN103207149 A CN 103207149A
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Abstract
The invention discloses an infrared gel positioning device and a gel imaging system utilizing the same. The infrared gel positioning device comprises an infrared sensor, an embedded control device, an external computer system and an external display screen. According to the invention, the infrared sensor is used for tracking the position of the gel on an objective table in real time, the position of displaying a gel image on a built-in display screen on the objective table is adjusted in real time according to the position information, so that the position and dimension of a stripe in the gel image can be completely overlapped with the position and dimension of the stripe of the gel on the objective table. By adopting the device and the system disclosed by the invention, the problem of wrong position corresponding relationship caused by sliding of colloid on a glass panel after the image generated by the gel imaging system is corresponding to the actual position of the colloid is solved, a gel fixing operation is omitted, and the working efficiency is improved.
Description
Technical field
The present invention relates to the gel imaging analysis technical field, relate in particular to a kind of infrared gel locating device and use the gel imaging system of this device.
Background technology
The gel imaging instrument is the Laboratory Instruments of electrophoresis result such as nucleic acid, protein being observed, take pictures, cutting glue, analysis, can do qualitative and semi-quantitative analysis to the separation and purification result of sample, be widely used in biology conventional study such as molecular weight calculating, density scan, density is quantitative.Because the Ago-Gel that gel electrophoresis is used is immersed in the electrophoresis liquid when electrophoresis, when taking out gel from electrophoresis liquid after, the experimenter is placed on the objective table, because the lubricated of electrophoresis liquid arranged between gel and the objective table glass, the experimenter is when utilizing gel imaging system to carry out the DNA recovery, gel very easily slides, and the image that produces of gel imaging system and the physical location of colloid misplace easily like this.
Summary of the invention
The objective of the invention is to by a kind of infrared gel locating device and use the gel imaging system of this device, solve the problem that above background technology is partly mentioned.
For reaching this purpose, the present invention by the following technical solutions:
A kind of infrared gel locating device, it comprises: infrared inductor, device for embedded control, external computer system and external display screen;
Described infrared inductor is arranged at around the objective table, be connected with device for embedded control, be used for launching infrared ray by infrared emission tube to objective table, receive on the objective table infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to the external computer system by device for embedded control;
Described device for embedded control is connected with the external computer system, is used for the control signal according to the output of external computer system, adjusts the position of built-in display screen demonstration gel images in the objective table;
Described external computer system is connected with external display screen, be used for the signal of infrared inductor output is analyzed, obtain the positional information of gel on objective table, and utilize this positional information to adjust the position that built-in display screen on the objective table shows gel images in real time, band position and the size of gel on band position in the gel images, size and the objective table are overlapped fully;
Described external display screen is connected with the external computer system, is used for showing the relevant operation information of this positioning system.
Especially, described infrared inductor is arranged on apart from 3 millimeters height of objective table.
Especially, described external display screen is selected any of LED display, LCD display, CRT display screen or rear-projection display screen for use.
The invention also discloses a kind of gel imaging system of using above-mentioned infrared gel locating device, it comprises: objective table, filming apparatus, infrared inductor, built-in display screen, device for embedded control, external computer system and external display screen;
Described filming apparatus is arranged at the objective table top, is connected with device for embedded control, is used for obtaining the gel images on the objective table;
Described infrared inductor is arranged at around the objective table, be connected with device for embedded control, be used for launching infrared ray by infrared emission tube to objective table, receive on the objective table infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to the external computer system by device for embedded control;
Described built-in display screen is arranged at objective table end face below, is connected with device for embedded control, is used for showing the gel images of external computer system output;
Described device for embedded control is connected with the external computer system, is used for the control signal according to the output of external computer system, control filming apparatus and built-in display screen;
Described external computer system is connected with external display screen, be used for when the UV-lamp of irradiation gel is opened, adjust the focal length of filming apparatus by device for embedded control, band position to gel, shape is taken, obtain first image of gel, when UV-lamp is closed, open built-in display screen by device for embedded control, at built-in display screen reference point is set, the control filming apparatus is taken gel location, obtain second image of gel, described first image and second image are handled, obtain the 3rd image, and the signal of analysis infrared inductor output, obtain the positional information of gel on objective table, utilize this positional information to adjust the position that built-in display screen on the objective table shows the 3rd image in real time, make the gel location of the 3rd image, size and band position, the position of gel on size and the objective table, size and band position, size is in full accord;
Described external display screen is used for showing the relevant operation information of this gel imaging system.
Especially, described external computer system also is used for carrying out error correction when there is distortion of the mesh in second image or is blocked.
Especially, described external computer system is further used for the band of described the 3rd image of gel is identified.
Especially, the centre of described objective table is clear glass.
Especially, described built-in display screen is any of LED display, LCD display, CRT display screen or rear-projection display screen.
Infrared gel locating device and gel imaging system that the present invention proposes carry out real-time follow-up by infrared inductor to the position of gel on objective table, adjust the position that built-in display screen on the objective table shows gel images in real time according to this positional information, band position and the size of gel on band position in the gel images, size and the objective table are overlapped fully.The invention solves colloid after the physical location correspondence of image that gel imaging system produces and colloid and produce the problem that causes position corresponding relation mistake after sliding at face glass, the operation of having saved immobilized gel has improved work efficiency.
Description of drawings
The infrared gel positioning device structure synoptic diagram that Fig. 1 provides for the embodiment of the invention;
The gel imaging system structural representation that Fig. 2 provides for the embodiment of the invention.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.Be understandable that specific embodiment described herein only is used for explaining the present invention, but not limitation of the invention.Also need to prove in addition, for convenience of description, only show part related to the present invention in the accompanying drawing but not full content.
Please refer to shown in Figure 1ly, infrared gel locating device specifically comprises in the present embodiment: infrared inductor 101, device for embedded control 102, external computer system 103 and external display screen 104.
Described infrared inductor 101 is arranged at around the objective table, be connected with device for embedded control 102, be used for launching infrared ray by infrared emission tube to objective table, receive on the objective table infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to external computer system 103 by device for embedded control 102.
In order better the position of gel on the objective table to be followed the tracks of, the quantity of the infrared inductor 101 that arranges around the objective table is The more the better, and described infrared inductor 101 is arranged on apart from 3 millimeters height of objective table in the present embodiment.
Described device for embedded control 102 is connected with external computer system 103, is used for the control signal according to 103 outputs of external computer system, adjusts the position of built-in display screen demonstration gel images in the objective table.
Described external computer system 103 is connected with external display screen 104, be used for the signal of infrared inductor 101 outputs is analyzed, obtain the positional information of gel on objective table, and utilize this positional information to adjust the position that built-in display screen on the objective table shows gel images in real time, band position and the size of gel on band position in the gel images, size and the objective table are overlapped fully.
Described external display screen 104 is connected with external computer system 103, is used for showing the relevant operation information of this positioning system.External display screen 104 is selected any of LED display, LCD display, CRT display screen or rear-projection display screen for use described in the present embodiment.
As shown in Figure 2, the gel imaging system of the above-mentioned infrared gel locating device of application comprises in the present embodiment: objective table 201, filming apparatus 202, infrared inductor 101, built-in display screen 2011, device for embedded control 102, external computer system 103 and external display screen 104.
Described filming apparatus 202 is arranged at objective table 201 tops, is connected with device for embedded control 102, is used for obtaining the gel images on the objective table 201.Filming apparatus 202 is made up of autozoom camera lens and digital CCD module in the present embodiment.The centre of described objective table 201 is clear glass.
Described infrared inductor 101 is arranged at around the objective table 201, be connected with device for embedded control 102, be used for by infrared emission tube to objective table 201 emission infrared rays, receive on the objective table 201 infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to external computer system 103 by device for embedded control 102.
Described built-in display screen 2011 is arranged at objective table 201 end faces below, is connected with device for embedded control 102, is used for showing the gel images of external computer system 103 outputs.Built-in display screen 2011 can be selected any of LED display, LCD display, CRT display screen or rear-projection display screen for use.
Described device for embedded control 102 is connected with external computer system 103, is used for the control signal according to 103 outputs of external computer system, control filming apparatus 202 and built-in display screen 2011.
Described external computer system 103 is connected with external display screen 104, be used for when the UV-lamp of irradiation gel is opened, adjust the focal length of filming apparatus 202 by device for embedded control 102, band position to gel, shape is taken, obtain first image of gel, when UV-lamp is closed, open built-in display screen 2011 by device for embedded control 102, at built-in display screen 2011 grid that spacing differs is set, 202 pairs of gel location of control filming apparatus are taken, obtain second image of gel, described first image and second image are handled, obtain the 3rd image, and the signal of analysis infrared inductor 101 outputs, obtain the positional information of gel on objective table 201, utilize this positional information to adjust the position of built-in display screen 2011 demonstrations the 3rd image on the objective table 201 in real time, make the gel location of the 3rd image, size and band position, the position of gel on size and the objective table 201, size and band position, size is in full accord.Described external display screen 104 is used for showing the relevant operation information of this gel imaging system.
When having distortion of the mesh in second image or being blocked, external computer system 103 will carry out correction process.Cause obscuring of gel sample when having many bands to reclaim, external computer system 103 can also identify the band of described the 3rd image of gel, conveniently cuts the glue operation.
Technical scheme of the present invention has solved colloid after the physical location correspondence of image that gel imaging system produces and colloid and has produced the problem that causes position corresponding relation mistake after sliding at face glass, and the operation of having saved immobilized gel improved work efficiency.Simultaneously, utilize gel imaging system that the present invention proposes when cutting the glue operation, can close quartz burner, the objective table of placing gel extracted out outside analyser, carry out freely cutting glue, eliminated the injury of ultraviolet ray to human body skin, simultaneously, comfort level, the degree of freedom of cutting the glue operation have been increased, shortened the irradiation time of ultraviolet ray to gel amplifying nucleic acid sample, thereby reduce the destruction to nucleic acid samples, the operator can utilize on the built-in display screen band is marked, and sample obscures when having avoided cutting the glue operation.
Notice that above-mentioned only is preferred embodiment of the present invention and institute's application technology principle.Skilled person in the art will appreciate that to the invention is not restricted to specific embodiment described here, can carry out various obvious variation for a person skilled in the art, readjust and substitute and can not break away from protection scope of the present invention.Therefore, though carried out comparatively detailed explanation by the present invention of above embodiment, the present invention is not limited only to above embodiment, under the situation that does not break away from the present invention's design, can also comprise more other equivalent embodiment, and scope of the present invention is determined by appended claim scope.
Claims (8)
1. one kind infrared gel locating device is characterized in that, comprising: infrared inductor, device for embedded control, external computer system and external display screen;
Described infrared inductor is arranged at around the objective table, be connected with device for embedded control, be used for launching infrared ray by infrared emission tube to objective table, receive on the objective table infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to the external computer system by device for embedded control;
Described device for embedded control is connected with the external computer system, is used for the control signal according to the output of external computer system, adjusts the position of built-in display screen demonstration gel images in the objective table;
Described external computer system is connected with external display screen, be used for the signal of infrared inductor output is analyzed, obtain the positional information of gel on objective table, and utilize this positional information to adjust the position that built-in display screen on the objective table shows gel images in real time, band position and the size of gel on band position in the gel images, size and the objective table are overlapped fully;
Described external display screen is connected with the external computer system, is used for showing the relevant operation information of this positioning system.
2. infrared gel locating device according to claim 1 is characterized in that, described infrared inductor is arranged on apart from 3 millimeters height of objective table.
3. infrared gel locating device according to claim 1 is characterized in that, described external display screen is selected any of LED display, LCD display, CRT display screen or rear-projection display screen for use.
4. an application rights requires the gel imaging system of 1 described infrared gel locating device, and it comprises: objective table, filming apparatus, infrared inductor, built-in display screen, device for embedded control, external computer system and external display screen;
Described filming apparatus is arranged at the objective table top, is connected with device for embedded control, is used for obtaining the gel images on the objective table;
Described infrared inductor is arranged at around the objective table, be connected with device for embedded control, be used for launching infrared ray by infrared emission tube to objective table, receive on the objective table infrared ray through the gel transmission by infrared receiver tube, and infrared ray changed handle, the signal after handling is exported to the external computer system by device for embedded control;
Described built-in display screen is arranged at objective table end face below, is connected with device for embedded control, is used for showing the gel images of external computer system output;
Described device for embedded control is connected with the external computer system, is used for the control signal according to the output of external computer system, control filming apparatus and built-in display screen;
Described external computer system is connected with external display screen, be used for when the UV-lamp of irradiation gel is opened, adjust the focal length of filming apparatus by device for embedded control, band position to gel, shape is taken, obtain first image of gel, when UV-lamp is closed, open built-in display screen by device for embedded control, at built-in display screen reference point is set, the control filming apparatus is taken gel location, obtain second image of gel, described first image and second image are handled, obtain the 3rd image, and the signal of analysis infrared inductor output, obtain the positional information of gel on objective table, utilize this positional information to adjust the position that built-in display screen on the objective table shows the 3rd image in real time, make the gel location of the 3rd image, size and band position, the position of gel on size and the objective table, size and band position, size is in full accord;
Described external display screen is used for showing the relevant operation information of this gel imaging system.
5. gel imaging system according to claim 4 is characterized in that, described external computer system also is used for carrying out error correction when there is distortion of the mesh in second image or is blocked.
6. gel imaging system according to claim 4 is characterized in that, described external computer system is further used for the band of described the 3rd image of gel is identified.
7. gel imaging system according to claim 4 is characterized in that, the centre of described objective table is clear glass.
8. according to the described gel imaging system of one of claim 4 to 7, it is characterized in that described built-in display screen is any of LED display, LCD display, CRT display screen or rear-projection display screen.
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| CN2013101333479A CN103207149A (en) | 2013-04-16 | 2013-04-16 | Infrared gel positioning device and gel imaging system utilizing same |
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| CN2013101333479A CN103207149A (en) | 2013-04-16 | 2013-04-16 | Infrared gel positioning device and gel imaging system utilizing same |
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Application publication date: 20130717 |