CN103205476B - A kind of method improving aureofuscin output - Google Patents
A kind of method improving aureofuscin output Download PDFInfo
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- CN103205476B CN103205476B CN201310126485.4A CN201310126485A CN103205476B CN 103205476 B CN103205476 B CN 103205476B CN 201310126485 A CN201310126485 A CN 201310126485A CN 103205476 B CN103205476 B CN 103205476B
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- fermention medium
- aureofuscin
- fermentation
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- sodium acetate
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Abstract
The present invention relates to a kind of method improving aureofuscin output.The technical scheme adopted is: in fermentor tank, brown for gold streptomycete is inoculated in fermention medium by the inoculum size of 8-12%, culture temperature 29 DEG C, mixing speed 220r/min, sodium acetate or the Sodium Propionate of 0.1-0.8% is added during the fermentation by weight percentage, fermentation 80-85h in fermention medium.The present invention, by optimizing fermention medium, adds the sodium acetate of 0.4% or the Sodium Propionate of 0.4% when fermentation 24h in fermention medium, and detect through high performance liquid phase, in the fermented liquid after fermentation, aureofuscin output can reach 2167.4 μ gmL
1.
Description
Technical field
The invention belongs to fermentation arts, relate to a kind of method improving aureofuscin output particularly.
Background technology
Aureofuscin (
aureofuscin) be streptomycete novel species-golden brown streptomycete (
streptomyces aureofuscus.n.sp) a kind of tetraene macrolide antifungal antibiotic produced, its chemical structure and foreign literature report tennecetin (
natamycin) structural similitude.Early stage research shows, aureofuscin has very strong anti-microbial effect to mould, multiple yeast and filamentous fungus, but not antibacterium, be used for the treatment of fungal keratitis clinically, its curative effect is better than amphotericin B, also has good curative effect to some fungus-caused tetter and colpitis mycoticas etc. simultaneously.Aureofuscin is furtherd investigate always and carries out industrialization development only made early stage chemical structure and the preliminary test of pesticide effectiveness in time within 1975, finding after.Existing fermentation process, aureofuscin extract yield is not high, is therefore difficult to reach industrialization development requirement.
Summary of the invention
The object of this invention is to provide a kind of method improving aureofuscin output.
The technical solution used in the present invention is: a kind of method improving aureofuscin output, method is as follows: in fermentor tank, brown for gold streptomycete is inoculated in fermention medium by the inoculum size of 8-12%, culture temperature 29 DEG C, mixing speed 220r/min, during the fermentation, in fermention medium, add sodium acetate or the Sodium Propionate of 0.1-0.8% by weight percentage, fermentation 80-85h.
Above-mentioned method, preferably, the inoculum size of golden brown streptomycete is 10%.
Above-mentioned method, preferably, fermention medium is: containing yeast powder 0.2g, Zulkovsky starch 1g in the fermention medium of every 1000ml.
Above-mentioned method, preferably, adds sodium acetate or Sodium Propionate when fermenting 24h in fermention medium.Preferred, in fermention medium, add the sodium acetate of 0.4% or the Sodium Propionate of 0.4% by weight percentage when fermenting 24h.
The invention has the beneficial effects as follows: the present invention is by optimizing fermention medium, in fermention medium, the sodium acetate of 0.4% or the Sodium Propionate of 0.4% is added when fermentation 24h, detect through high performance liquid phase, in the fermented liquid after fermentation, aureofuscin output can reach 2167.4 μ g mL
1.
Embodiment
embodiment 1
In fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium (fermention medium be: containing yeast powder 0.2g in the fermention medium of every 1000ml, Zulkovsky starch 1g) in, culture temperature 29 DEG C, mixing speed 220r/min, at the fermentation initial stage respectively in fermention medium, add the sodium acetate of 0.1%, Sodium Propionate, Sodium propanecarboxylate, ethanol, n-propyl alcohol and propyl carbinol by weight percentage, not add these materials as contrast, ferment after 84 h and sample, detect aureofuscin output by high performance liquid phase, the results are shown in Table 1.
Table 1
From table 1, when adding sodium acetate or Sodium Propionate, aureofuscin output control group of comparing obviously increases.
embodiment 2
In fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium (fermention medium be: containing yeast powder 0.2g in the fermention medium of every 1000ml, Zulkovsky starch 1g) in, culture temperature 29 DEG C, mixing speed 220r/min, at the fermentation initial stage respectively in fermention medium, add the sodium acetate of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8% by weight percentage, not add sodium acetate in contrast, ferment after 84 h and sample, detect aureofuscin output by high performance liquid phase, result is as table 2.
Table 2
From table 2, when adding the sodium acetate of 0.4%, aureofuscin output is compared maximum.
embodiment 3
In fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium (fermention medium be: containing yeast powder 0.2g in the fermention medium of every 1000ml, Zulkovsky starch 1g) in, culture temperature 29 DEG C, mixing speed 220r/min, at the 0th, 24,36,48,60,72 hour of fermentation culture, respectively in fermention medium, add the sodium acetate of 0.4 % by weight percentage, not add sodium acetate as contrast, ferment after 84 h and sample, detect aureofuscin output by high performance liquid phase, the results are shown in Table 3.
Table 3
From table 3, be add sodium acetate constantly fermentation 24 is little, aureofuscin output is compared maximum.
embodiment 4
In fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium, culture temperature 29 DEG C, mixing speed 220r/min, at the 24th hour of fermentation culture, respectively in fermention medium, add the sodium acetate of 0.4 % by weight percentage, not add sodium acetate as contrast, sample after 84 h that ferment, detect aureofuscin output by high performance liquid phase, the results are shown in Table 4.
Fermention medium 1: containing yeast powder 0.2g, Zulkovsky starch 1g in the fermention medium of every 1000ml.
Fermention medium 2: containing glucose 4g, W-Gum 2g in the fermention medium of every 1000ml, peptone 0.13g, analysis for soybean powder 1g, MgSO
47H
2o 0.05g, CaCO
30.6g.
Table 4
From table 4, although both output is close, fermentative medium formula of the present invention is simple, economical, convenient, and has good culture effect.
embodiment 5
In fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium (containing yeast powder 0.2g in the fermention medium of every 1000ml, Zulkovsky starch 1g) in, culture temperature 29 DEG C, mixing speed 220r/min, at the 24th hour of fermentation culture, to in fermention medium, add the Sodium Propionate of 0.4 % by weight percentage, sample after 84 h that ferment, detecting aureofuscin output by high performance liquid phase is 2105.3 μ g mL
1.
Control group---same fermentation condition, but do not add Sodium Propionate, aureofuscin output about 600 μ g mL
1, visible the present invention by improving fermentation condition, after adding the Sodium Propionate of 0.4%, aureofuscin output increased about 2.5 times.
Claims (1)
1. one kind is improved the method for aureofuscin output, it is characterized in that method is as follows: in fermentor tank, by brown for gold streptomycete by 10% inoculum size be inoculated in fermention medium, culture temperature 29 DEG C, mixing speed 220r/min, when fermenting 24h in fermention medium, add sodium acetate or the Sodium Propionate of 0.4% by weight percentage, fermentation 84h; Described fermention medium is: containing yeast powder 0.2g, Zulkovsky starch 1g in the fermention medium of every 1000ml.
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| CN201310126485.4A CN103205476B (en) | 2013-04-12 | 2013-04-12 | A kind of method improving aureofuscin output |
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| CN103205476A CN103205476A (en) | 2013-07-17 |
| CN103205476B true CN103205476B (en) | 2015-08-12 |
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| CN107858387A (en) * | 2017-12-12 | 2018-03-30 | 山东福瑞达生物科技有限公司 | A kind of preparation method of high-dissolvability natamycin |
| CN108531488B (en) * | 2018-03-30 | 2022-06-14 | 辽宁大学 | Novel regulatory gene AurT and application thereof in increasing yield of aureofuscin |
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Non-Patent Citations (2)
| Title |
|---|
| 魏杰等.前体对金褐霉素生物合成的影响.《食品与生物技术学报》.2009,第28卷(第3期),摘要,第430页2.1节. * |
| 魏杰等.金褐霉素高产菌株的选育及发酵条件的优化.《食品与生物技术学报》.2009,第28卷(第2期),240-242. * |
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