CN103205389B - Streptomyces flavotricini for antagonizing botryosphaeria dothidea - Google Patents
Streptomyces flavotricini for antagonizing botryosphaeria dothidea Download PDFInfo
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- CN103205389B CN103205389B CN201310176547.2A CN201310176547A CN103205389B CN 103205389 B CN103205389 B CN 103205389B CN 201310176547 A CN201310176547 A CN 201310176547A CN 103205389 B CN103205389 B CN 103205389B
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Abstract
The invention relates to biological prevention of plant diseases and belongs to the field of biological prevention research and application in a plant protection subject. The invention discloses streptomyces flavotricini YZF3 for antagonizing botryosphaeria dothidea, with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7529. The strain can effectively inhibit the botryosphaeria dothidea and the effect is obviously better than that of bismerthiazol and is equivalent to agricultural streptomycin; and the strain has the potential of preventing and treating the botryosphaeria dothidea. Compared with a chemical medicament, the YZF3 strain is used for preventing and treating the plant diseases and has no problems of environmental pollution, pesticide residues and the like.
Description
Technical field
The present invention relates to the biological control of Plant diseases, belong to biological control research and Application Areas in plant protection subject.
Background technology
Red bayberry Peptic Ulcers is the important disease of many red bayberry growing areas, very easily causes tree vigo(u)r weak, affects red bayberry yield and quality.The method of this disease of control is mainly to utilize chemical agent bismerthiazol and agricultural streptomycin to smear at present, and other pharmacy control efficacies are all not ideal enough.From disease control angle, only two kinds of medicaments are abundant not for disease control, also need to develop other beneficial agents for agriculture production choice for use.For basis being provided to development of new biological pesticide, the medicament kind of abundant control red bayberry Peptic Ulcers, in the present invention, from soil, screened a strain actinomycetes YZF3 bacterial strain, it can effectively suppress red bayberry ulcer bacteria (Pseudomonas syringae pv.myricae) growth.Through identifying that this biocontrol microorganisms is for yellow three plain streptomycetes (Streptomyces flavotricini).Inhibition and the agricultural streptomycin of this bacterial strain to red bayberry ulcer bacteria is suitable, is obviously better than chemical agent bismerthiazol.Therefore can be with yellow three plain streptomycete YZF3 research and development novel biopesticides, for the biological control of red bayberry Peptic Ulcers.
Summary of the invention
For the few problem of medicament kind of control red bayberry Peptic Ulcers, the yellow three plain streptomycete YZF3 of Biocontrol microorganism are screened.Inhibition and the agricultural streptomycin of this bacterial strain to red bayberry ulcer bacteria is suitable, is better than chemical agent bismerthiazol.Complete at present the inhibition of germ has been measured and Identification of Species.
Nature is containing many beneficial microorganisms, screening in a large number from environment, and obtaining Biocontrol microorganism is the element task of biological agent development, is also usual in the industry operating mode.Actinomycetes are that a class produces antibiotic mushroom, and taking red bayberry ulcer bacteria as target bacteria, from soil, screening can suppress the actinomycetes of this bacterium, is the method for the screening Biocontrol microorganism that a kind of efficiency is higher.
The invention discloses yellow three plain streptomycetes (Streptomyces flavotricini) YZF3 of a kind of antagonism red bayberry ulcer bacteria, its deposit number is CGMCC No.7529.
Yellow three plain streptomycete YZF3 bacterial strains can effectively suppress red bayberry ulcer bacteria, and successful is better than bismerthiazol, suitable with agricultural streptomycin, have the potentiality of control red bayberry Peptic Ulcers.Compared with chemical agent, use YZF3 bacterial strain controlling plant diseases there is no the problem such as environmental pollution, pesticide residue.
Brief description of the drawings Fig. 1 is the antagonistic effects of yellow three plain streptomycete YZF3 bacterial strains to red bayberry ulcer bacteria.
Fig. 2 is the thalli morphology (× 1000) of YZF3 bacterial strain.
YZF3 bacterial strain of the present invention is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 28th, 2013, (No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), Classification And Nomenclature is yellow three plain streptomycetes (Streptomyces flavotricini), and deposit number is CGMCC7529.
Embodiment
(1) gather soil sample, remove the large particulate matter such as stone, pulverize air-dryly, sieve.
(2) 20g sample is positioned in 200mL sterilized water, fully concussion, and taking this liquid as mother liquor, gradient dilution.
(3) get 10
-6diluent 0.1mL coating Gause I flat board.
Under (4) 28 DEG C of conditions, cultivate 7 days.
(5) single bacterium colony of separation different colours, proterties, preserves.
(6) by red bayberry ulcer bacteria, (bacterial strain is separated and obtains from Cixi City red bayberry garden diseased plant by this laboratory, be red bayberry ulcer bacteria through form and molecular biology identification) mix with the bacteria culture medium of 30 DEG C, after cooling, make flat board, on flat board, punch, cut out agar block, in hole, splash into the bacteria suspension 20 μ L of isolated strains, 28 DEG C of cultivations, observe inhibition zone size.(7) remain with the bacterial strain of inhibition zone, carry out single bacterium colony purifying.
(8) turn continuously Dai Duodai, survey again the inhibition zone of bacterial strain by (6) method, determine antagonistic stability.The bacterial strain called after YZF3 filtering out.As Fig. 1.In figure in culture dish control wells (hole, lower-left) around germ cover with, other 3 holes have dripped YZF3 bacterium liquid, around it, having formed obvious blank ring is inhibition zone, illustrates that in YZF3 strain fermentation process, can produce inhibitory substance can not grow germ around.
(9) YZF3 bacterial strain is carried out to Identification of Species.
(10) adopt Physiology and biochemistry (morphological specificity, cultural characteristic and physiological and biochemical property) and molecular biology method (16S rRNA gene sequencing) to carry out bacterial strain Identification of Species.
Morphological specificity: Gram-positive; On ISP2 agar, ISP3 agar, ISP4 agar, ISP5 agar and Bennett ' s nutrient agar, grow after 7 days, substrate mycelium physically well develops, and without tabula, does not rupture; Aerial hyphae well-grown, multiple-limb; Fibrillae of spores is straight, flexible, spore circle or oval, smooth (Fig. 2).
Cultural characteristic: the cultural characteristic of bacterial strain YZF3 is in table 1:
The cultural characteristic of table 1 bacterial strain YZF3
| Substratum | Substrate mycelium | Aerial hyphae | Soluble pigment |
| Bennett ' s substratum | Faint yellow | Canescence | Nothing |
| ISP2 substratum | Faint yellow | Pink is to white | Nothing |
| ISP3 substratum | Faint yellow | Pink | Nothing |
| ISP4 substratum | Faint yellow | Pink is to white | Nothing |
| ISP5 substratum | Faint yellow | Without gas silk | Nothing |
Physiological and biochemical property: the physiological and biochemical property of bacterial strain is in table 2.
The physiological and biochemical property of table 2 bacterial strain YZF3
+, the positive;-, feminine gender
Above feature is consistent with streptomycete
16S rRNA gene sequencing: extract the full genome of YZF3, with the amplification of 16S rRNA universal primer, carry out Blast comparison after the gene order order-checking of amplification in GenBank, result shows that bacterial strain YZF3 belongs to streptomyces; Compare to the relevant bacterial strain of delivering at present, the 16S rRNA gene order of bacterial strain YZF3 is very high with the sequence similarity of yellow three plain streptomycetes (Streptomyces flavotricini), is 99.8%.YZF3 bacterial strain is yellow three plain streptomycetes.
Claims (2)
1. a bacterial strain for antagonism red bayberry ulcer bacteria, it is yellow three plain streptomycetes (Streptomyces flavotricini) YZF3 of bacterium, its deposit number is CGMCC No.7529.
2. the application of yellow three plain streptomycetes (Streptomyces flavotricini) CGMCC No.7529 in control red bayberry Peptic Ulcers.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310176547.2A CN103205389B (en) | 2013-05-13 | 2013-05-13 | Streptomyces flavotricini for antagonizing botryosphaeria dothidea |
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| CN201310176547.2A CN103205389B (en) | 2013-05-13 | 2013-05-13 | Streptomyces flavotricini for antagonizing botryosphaeria dothidea |
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| CN103205389B true CN103205389B (en) | 2014-06-18 |
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| CN103966133B (en) * | 2014-05-13 | 2017-06-16 | 北京林业大学 | One streptomycete and its application |
| CN107058178B (en) * | 2017-03-13 | 2020-08-04 | 陕西博秦生物工程有限公司 | Streptomyces flavetris, microbial inoculum and application thereof |
| CN110093295B (en) * | 2019-05-15 | 2022-08-30 | 湖南师范大学 | Streptomyces flaviviridis for resisting fish pathogenic bacteria and application thereof |
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Granted publication date: 20140618 Termination date: 20170513 |