CN103175969B - The kit of auxiliary diagnosis From Lung Squamous Carcinoma Patients - Google Patents
The kit of auxiliary diagnosis From Lung Squamous Carcinoma Patients Download PDFInfo
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- CN103175969B CN103175969B CN201210209867.9A CN201210209867A CN103175969B CN 103175969 B CN103175969 B CN 103175969B CN 201210209867 A CN201210209867 A CN 201210209867A CN 103175969 B CN103175969 B CN 103175969B
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Abstract
The invention discloses a kind of kit of auxiliary diagnosis From Lung Squamous Carcinoma Patients.Kit provided by the invention comprise product for detecting protein marker IDH1, for detect protein marker CA125 product, for detecting the product of protein marker CYFRA21-1 and recording the carrier of following functional expression:
Description
Technical field
The present invention relates to a kind of kit of auxiliary diagnosis From Lung Squamous Carcinoma Patients.
Background technology
Lung cancer is serious threat human life and healthy major disease.2008 world statistics data results displays of the World Health Organization (WHO) (WHO) international cancer research institution IARC announcement in 2011: lung cancer is the malignant tumour that M & M all ranks first.Annual global neopathy number of cases 160.8 ten thousand, death number 137.7 ten thousand, accounts for 12.7% and 18.2% of whole malignant tumour.The statistical result showed that American Cancer Society announces: estimate in 2012, pulmonary carcinosis number of cases is newly sent out by the U.S. will reach 22.6 ten thousand, because lung cancer death case load will reach 160,000.In China, the whole nation third time cause of the death Retrospect spot-check result of being presided over by the Ministry of Public Health shows: lung cancer is that first of the Death Cause for Malignant Tumors of sample regions, crude death rate is 30.83/10 ten thousand, wherein the male sex 41.34/10 ten thousand, and women 19.84/10 ten thousand; The first cancer mortality reason is in the male sex, women.Along with the factor such as acceleration and increasing environmental pollution of global aging, lung cancer morbidity will continue in rising trend, therefore, to the diagnoses and treatment progress of research demand of lung cancer, and must be more and more stronger.
From pathology, lung cancer can be divided into non-small cell lung cancer (NSCLC accounts for 85%) and small-cell carcinoma of the lung (SCLC accounts for 15%).Wherein, NSCLC forms primarily of squama cancer (also known as squamous cell carcinoma or lung squamous cancer), gland cancer (also known as adenocarcinoma of lung) and large cell carcinoma.Squama cancer and gland cancer respectively account for 40% of NSCLC.
The morbidity of lung cancer is comparatively hidden, and the patient more than 70% is developed to middle and advanced stage when medical, loses surgical engine meeting.This is also lung cancer weak curative effect, the one of the main reasons that mortality ratio is high.Therefore, early screening, diagnosis become the most important thing of preventing and treating lung cancer.At present, the Imaging Technologies such as clinical used screening lung cancer diagnostic method mainly chest X-ray inspection, CT scan.Although these image datas play an important role to diagnosis, also there is a lot of limitation, such as false positive rate is high, cannot detect recessive focus, subclinical focus and small metastatic lesion.In addition, also comprising bronchoscopy, aspiration biopsy etc. to the diagnostic method of lung cancer has wound to operate, these class methods length consuming time, increases patient suffering.Therefore, find without wound, radiationless, low cost, diagnosis fast and all high examination of susceptibility, specificity, diagnostic method, be the active demand of current early diagnosis of cancer.
Tumor marker refers to the bioactivator that tumour cell or tissue produce due to oncogene or other tumor-related genes and product unconventionality expression thereof or itself is come off by cancerous tissue, and have when normal structure or benign disease to a certain degree express or output very micro-.It reflects the generation evolution of cancer, can organize at tumor patient, detect in body fluid and excreta, be widely used in the diagnosis of tumour, monitoring recurrence, transfer, prognosis, prediction curative effect etc.The existing several outstanding feature things extensively approved are clinically: the tumour antigen 125(CA125 being applied to oophoroma), be applied to the class antigen 1 9-9 (CA19-9) of cancer of pancreas, be applied to the carcinomebryonic antigen (CEA) of colorectal cancer and be applied to the prostate specific antigen (PSA) of prostate cancer.And other cancers such as breast cancer and lung cancer, still lack the believable tumor marker for examination at present.
The label being applied to lung cancer clinical auxiliary diagnosis at present only has protein marker, for following several:
(1) first CEA:Gold and Freeman find CEA in nineteen sixty-five in colorectal cancer, and it is a kind of glycoprotein, and molecular weight is 180KD, and the half life period is 3-4 days, is the antigen that developing embryo process produces, significantly declines after birth; Its specificity is strong, but can be used for the monitoring of tumor development, Outcome measure and prognosis and estimate, insensitive for early diagnosis; Though lung cancer patient has rising, there is higher false negative and false positive, be unsuitable for being used alone in screening lung cancer diagnosis;
(2) first CA125:Bast finds this large glycoproteins of CA125 1981, about 4.8 days its half life period, is mainly used in oophoroma, also has rising in lung cancer simultaneously;
(3) CYFRA21-1(CYFRA211): CYFRA211 is the water-soluble fragment of Cyfra21-1, about 4 days half life period;
Although above protein marker is for the auxiliary diagnosis of lung cancer at present, its diagnosis efficiency is low, does not reach clinical demand.Therefore, new diagnostic marker is developed and diagnostic method is too impatient to wait.
IDH1 is the isocitric dehydrogenase that a kind of NADP+ being arranged in tenuigenin and peroxisome relies on, and can carry out oxidative deamination reaction for α-ketoglutaric acid by catalysis isocitric acid.The present inventor finds in research in the past, and the IDH1 level of lung squamous cancer and patients with lung adenocarcinoma, can as the new diagnostic marker of lung cancer apparently higher than normal population and benign tumor of lung patient.
Summary of the invention
The object of this invention is to provide a kind of kit of auxiliary diagnosis From Lung Squamous Carcinoma Patients.
Kit for auxiliary diagnosis From Lung Squamous Carcinoma Patients provided by the invention, comprise the product for detecting protein marker IDH1, for detect protein marker CA125 product, for detecting the product of protein marker CYFRA21-1 and recording the carrier of following functional expression:
X1 represents the concentration of protein marker IDH1, and x2 represents the concentration of protein marker CA125, and x3 represents the concentration of protein marker CYFRA21-1.
Described protein marker IDH1 specifically can be the protein (sequence 1 of sequence table shown in GENBANK ACCESSION NO.CAG46496.1; GENBANK ACCESSION NO.CAG46496.1; GI:49456351; Linear PRI16-OCT-2008).Described protein marker CA125 specifically can be protein (the GENBANK ACCESSION NO.AAL65133.2 shown in GENBANK ACCESSION NO.AAL65133.2; GI:24419041; Linear PRI29-OCT-2002).Described protein marker CYFRA21-1 specifically can be the protein (sequence 2 of sequence table shown in GENBANK ACCESSIONNO.NP 002267.2; GI:24234699; Linear PRI06-MAY-2012).
The unit of the concentration of described protein marker IDH1 is specially U/L, and the unit of the concentration of described protein marker CA125 is specially U/mL, and the unit of the concentration of described protein marker CYFRA21-1 is specially ng/mL.
Described carrier also can record following diagnostic criteria: patient to be measured is the From Lung Squamous Carcinoma Patients of candidate when p >=0.5775, as p < 0.5775, patient to be measured is the non-From Lung Squamous Carcinoma Patients of candidate.
The present invention also protect the product for detecting protein marker IDH1, for detect protein marker CA125 product, for detecting the product of protein marker CYFRA21-1 and recording the application of carrier in the kit preparing auxiliary diagnosis From Lung Squamous Carcinoma Patients of functional expression and diagnostic criteria:
Described functional expression is as follows:
X1 represents the concentration of protein marker IDH1, unit is that U/L, x2 represent the concentration of protein marker CA125, unit is that U/mL, x3 represent the concentration of protein marker CYFRA21-1, unit is ng/mL;
Described diagnostic criteria is as follows:
When p >=0.5775, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, and as p < 0.5775, patient to be measured is the non-From Lung Squamous Carcinoma Patients of candidate.
Described protein marker IDH1 specifically can be the protein (sequence 1 of sequence table shown in GENBANK ACCESSION NO.CAG46496.1; GENBANK ACCESSION NO.CAG46496.1; GI:49456351; Linear PRI16-OCT-2008).Described protein marker CA125 specifically can be protein (the GENBANK ACCESSION NO.AAL65133.2 shown in GENBANK ACCESSION NO.AAL65133.2; GI:24419041; Linear PRI29-OCT-2002).Described protein marker CYFRA21-1 specifically can be the protein (sequence 2 of sequence table shown in GENBANK ACCESSIONNO.NP 002267.2; GI:24234699; Linear PRI06-MAY-2012).
Arbitraryly above described specifically can be IDH1 enzyme-linked immunosorbent assay kit (ELISA Kit purchased from Wuhan You Ersheng Science and Technology Ltd. for the product detecting protein marker IDH1; Comprising IDH1 standard items).
Arbitraryly above described specifically can be CYFRA21-1 quantitative determination reagent kit purchased from Roche Diagnistics product (Shanghai) Co., Ltd. for the product detecting protein marker CYFRA21-1.
Arbitrary described CA125 quantitative determination reagent kit that specifically can be Roche Diagnistics product (Shanghai) Co., Ltd. for the product detecting protein marker CA125 above.
Lung cancer occupy the first place of world's cancer mortality reason, is also the No.1 cancer killer of harm our people health.Therefore, find the tumor marker that can be used for lung cancer early diagnosis, individual treatment, prognosis and monitoring recurrence, become the focus of research in recent years.Adopt kit provided by the invention and according to corresponding diagnostic criteria auxiliary diagnosis From Lung Squamous Carcinoma Patients, have that sensitivity is high, the feature of high specificity, diagnostic result confidence level is diagnosed higher than each independent protein label of employing far away, and the Diagnosis and Treat for lung squamous cancer has substantial worth and application prospect.
Accompanying drawing explanation
Fig. 1 is the ROC curve of lung squamous cancer diagnostic model.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.Sensitivity: be diagnosed as the number percent that positive cancer patient's number accounts for total cancer patient's number.Specificity: be diagnosed as the number percent that negative Healthy People number accounts for total healthy population number.
The foundation of embodiment 1, the inventive method
One, the foundation of model
From Lung Squamous Carcinoma Patients plasma sample is totally 489 examples, and Healthy People (normal control) plasma sample is totally 479 examples, take from Cancer Hospital of Chinese Academy of Medical Sciences thoracic surgery go to a doctor and through definitive pathological diagnosis patients with lung cancer and participate in the normal population of Carcinoma screening.This experiment is agreed to through Ethics Committee, and experimenter is all informed disease signature Informed Consent Form by the know.In From Lung Squamous Carcinoma Patients, the male sex 466 example, women 23 example, the range of age be 28-86 year, the mean age is 61.56 years old; In normal population, the male sex 240 example, women 239 example, the range of age be 41-70 year, the mean age is 57.39 years old.Plasma sample obtains all before surgery, and patient does not accept the treatments such as chemotherapy, radiotherapy, intervention before this.
Data processing uses curve expert 1.4 cleanup standard curve; GraphPad Prism 5.01 processes Column statistical graph; SPSS13.0 carries out data fitting, founding mathematical models; SAS 9.2 carries out mathematical model inspection; MedCalc9.6.2.0 version computation model ROC curve and cutoff value; Adobe illustrator CS3 carries out picture processing.
ROC curve (receiver operating characteristic curve, receiver operating curves) be ordinate (Y-axis) with sensitivity, take specificity as horizontal ordinate (X-axis), when giving test different critical value (cutoff), corresponding coordinate points can be produced, be linked to be curve and ROC curve that line produces.ROC curve can the diagnosis efficiency of more different tumor markers, and area under curve (AUC) larger person diagnosis efficiency is higher.
Return regretional analysis data by the Logistic binomial of SPSS software, and use SAS to carry out model testing, finally determine a model, for the diagnosis of lung squamous cancer.The result of calculation of this model is drawn ROC curve (Fig. 1), area under curve is 0.905(95% fiducial interval: 0.885-0.923, p < 0.0001), with the label the highest to lung squamous cancer diagnosis efficiency---CYFRA211(AUC is 0.839) compared with, there is significant difference (p < 0.001).
In the specific descriptions step one of two, model,
By carrying out the detection of each protein marker to a large amount of clinical sample, carry out mass data simulation and model foundation, the functional expression obtained for auxiliary diagnosis lung squamous cancer is as follows:
X1 represents the concentration (unit is U/L) of protein marker IDH1, and x2 represents the concentration (unit is U/mL) of protein marker CA125, and x3 represents the concentration (unit is ng/mL) of protein marker CYFRA21-1.
Method based on above-mentioned functional expression auxiliary diagnosis lung squamous cancer is as follows:
(1) get the peripheric venous blood under patient's fasted conditions to be measured, room temperature leaves standstill 1h, and then the centrifugal 15min of 3000r/min, gets supernatant, and packing 1.5mL EP manages ,-80 DEG C of Refrigerator stores.
(2) protein marker IDH1 concentration, protein marker CA125 concentration and protein marker CYFRA211 concentration in the sample that obtains of detecting step (1), then p value is calculated by above-mentioned functional expression, when p >=0.5775, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, as p < 0.5775 patient to be measured for candidate be non-From Lung Squamous Carcinoma Patients.
The checking of embodiment 2, the inventive method
Patient for the present embodiment is that (peripheric venous blood obtains all before surgery for the From Lung Squamous Carcinoma Patients of 150 routine clinical definites, patient does not accept the treatments such as chemotherapy, radiotherapy, intervention before this) and 50 routine Healthy Peoples (non-From Lung Squamous Carcinoma Patients), be the volunteer of informed consent.
1, get the 5ml peripheric venous blood under patient's fasted conditions to be measured, room temperature leaves standstill 1h, and then the centrifugal 15min of 3000r/min, gets supernatant, is sub-packed in two 1.5mL EP and manages ,-80 DEG C of Refrigerator stores.
2, the sample 3h of dissolving step 1 at room temperature, the then centrifugal 5min of 3000r/min.
3, protein marker IDH1 concentration (unit is U/L), protein marker CA125 concentration (unit is U/mL), protein marker CYFRA211 concentration (unit is ng/mL) and protein marker CEA concentration (unit is ng/mL) in the sample (supernatant) that obtains of detecting step 2.
Described protein marker IDH1 specifically can be the protein (sequence 1 of sequence table shown in GENBANK ACCESSION NO.CAG46496.1; GENBANK ACCESSION NO.CAG46496.1; GI:49456351; Linear PRI16-OCT-2008).Described protein marker CA125 specifically can be protein (the GENBANK ACCESSION NO.AAL65133.2 shown in GENBANK ACCESSION NO.AAL65133.2; GI:24419041; Linear PRI29-OCT-2002).Described protein marker CYFRA21-1 specifically can be the protein (sequence 2 of sequence table shown in GENBANK ACCESSIONNO.NP 002267.2; GI:24234699; Linear PRI06-MAY-2012).Protein marker CEA specifically can be the protein (sequence 3 of sequence table shown in GENBANK ACCESSION NO.CAE75559.1; GENBANK ACCESSION NO.CAE75559.1; GI:38522495; LinearPAT 25-NOV-2003).
Adopt IDH1 enzyme-linked immunosorbent assay kit (the ELISA Kit purchased from Wuhan You Ersheng Science and Technology Ltd.; Comprising IDH1 standard items; Catalog number is E97839Hu 96T) and the concentration of protein marker IDH1 is detected by the explanation of kit.
Adopt the concentration of the CYFRA21-1 quantitative determination reagent kit (catalog number is 11820966122) purchased from Roche Diagnistics product (Shanghai) Co., Ltd. the explanation detection protein marker CYFRA21-1 by kit.
Adopt the concentration of the CEA quantitative determination reagent kit of Roche Diagnistics product (Shanghai) Co., Ltd. the explanation detection protein marker CEA by kit (catalog number is 11731629322).
Adopt the concentration of the CA125 quantitative determination reagent kit (catalog number is 11776223822) of Roche Diagnistics product (Shanghai) Co., Ltd. the explanation detection protein marker CA125 by kit.
4, protein marker IDH1 concentration step 3 obtained, protein marker CA125 concentration and protein marker CYFRA21-1 concentration substitute into the functional expression of embodiment 1, obtain p value, judge whether patient is From Lung Squamous Carcinoma Patients according to the standard of embodiment 1.
The results are shown in Table in 1 and table 2(sex of step 3 and step 4,1 represents the male sex, and 2 represent women).
In table 1 and table 2, using according to the diagnostic result of each single protein marker as the contrast adopting the functional expression of embodiment 1 to carry out the result diagnosed.During independent employing protein marker IDH1, conveniently threshold value, when concentration >=2.0874, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, as concentration < 2.0874 patient to be measured for candidate be non-From Lung Squamous Carcinoma Patients.During independent employing protein marker CA125, conveniently threshold value, when concentration >=35, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, as concentration < 35 patient to be measured for candidate be non-From Lung Squamous Carcinoma Patients.During independent employing protein marker CYFRA21-1, conveniently threshold value, when concentration >=3.3, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, as concentration < 3.3 patient to be measured for candidate be non-From Lung Squamous Carcinoma Patients.During independent employing protein marker CEA, conveniently threshold value, when concentration >=5, patient to be measured is the From Lung Squamous Carcinoma Patients of candidate, as concentration < 5 patient to be measured for candidate be non-From Lung Squamous Carcinoma Patients.
From Lung Squamous Carcinoma Patients (+representative diagnosis is positive, and-representative diagnosis is negative) made a definite diagnosis by table 1
Healthy person (+representative diagnosis is positive, and-representative diagnosis is negative) made a definite diagnosis by table 2
Carry out result judgement according to the functional expression of embodiment 1 and diagnostic method, sensitivity is 75.33%, and specificity is 90%.Independent employing protein marker IDH1 and threshold value thereof carry out result judgement, and sensitivity is 41.33%, and specificity is 90%.Independent employing protein marker CA125 and threshold value thereof carry out result judgement, and sensitivity is 16.67%, and specificity is 98%.Independent employing protein marker CYFRA21-1 and threshold value thereof carry out result judgement, and sensitivity is 70.67%, and specificity is 82%.Independent employing protein marker CEA and threshold value thereof carry out result judgement, and sensitivity is 16.67%, and specificity is 72%.Consider sensitivity and specificity, adopt the functional expression of embodiment 1 and diagnostic method to be significantly better than adopting several independent protein marker to diagnose.
Claims (2)
1. for detect protein marker IDH1 product, for detect protein marker CA125 product, for detecting the product of protein marker CYFRA21-1 and recording the application of carrier in the kit preparing auxiliary diagnosis From Lung Squamous Carcinoma Patients of functional expression and diagnostic criteria:
Described functional expression is as follows:
X
1represent the concentration of protein marker IDH1, unit is U/L, x
2represent the concentration of protein marker CA125, unit is U/mL, x
3represent the concentration of protein marker CYFRA21-1, unit is ng/mL.
2. apply as claimed in claim 1, it is characterized in that: described protein marker IDH1 is the protein shown in GENBANKACCESSION NO.CAG46496.1; Described protein marker CA125 is the protein shown in GENBANK ACCESSIONNO.AAL65133.2; Described protein marker CYFRA21-1 is the protein shown in GENBANK ACCESSIONNO.NP_002267.2.
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| CN104818322B (en) * | 2015-04-02 | 2017-08-04 | 中国医学科学院肿瘤医院 | MiRNA and Cyfra21 1 combine the application in detection non-small cell lung cancer |
| CN106350582B (en) * | 2016-08-25 | 2020-02-14 | 朱伟 | Serum miRNA marker related to lung squamous carcinoma auxiliary diagnosis and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2071337A1 (en) * | 2007-12-10 | 2009-06-17 | F.Hoffmann-La Roche Ag | Seprase as a marker for cancer |
| CN102321760A (en) * | 2011-08-26 | 2012-01-18 | 泸州医学院附属医院 | Non-small cell lung cancer (NSCLC) marker and its application |
| CN102414565A (en) * | 2009-05-04 | 2012-04-11 | 霍夫曼-拉罗奇有限公司 | Use of DPPIV/Seprase as a marker for cancer |
| WO2012054732A2 (en) * | 2010-10-20 | 2012-04-26 | Rush University Medical Center | Lung cancer tests |
| WO2012150959A1 (en) * | 2011-05-04 | 2012-11-08 | Abbott Laboratories | Methods for predicting sensitivity to treatment with a targeted tyrosine kinase inhibitor |
| CN102841201A (en) * | 2012-08-31 | 2012-12-26 | 马鞍山微因泰克生物科技有限公司 | Combined general check protein chip for early-stage cancers mainly comprising lung cancer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9347945B2 (en) * | 2005-12-22 | 2016-05-24 | Abbott Molecular Inc. | Methods and marker combinations for screening for predisposition to lung cancer |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2071337A1 (en) * | 2007-12-10 | 2009-06-17 | F.Hoffmann-La Roche Ag | Seprase as a marker for cancer |
| CN102414565A (en) * | 2009-05-04 | 2012-04-11 | 霍夫曼-拉罗奇有限公司 | Use of DPPIV/Seprase as a marker for cancer |
| WO2012054732A2 (en) * | 2010-10-20 | 2012-04-26 | Rush University Medical Center | Lung cancer tests |
| WO2012150959A1 (en) * | 2011-05-04 | 2012-11-08 | Abbott Laboratories | Methods for predicting sensitivity to treatment with a targeted tyrosine kinase inhibitor |
| CN102321760A (en) * | 2011-08-26 | 2012-01-18 | 泸州医学院附属医院 | Non-small cell lung cancer (NSCLC) marker and its application |
| CN102841201A (en) * | 2012-08-31 | 2012-12-26 | 马鞍山微因泰克生物科技有限公司 | Combined general check protein chip for early-stage cancers mainly comprising lung cancer |
Non-Patent Citations (1)
| Title |
|---|
| Fengwei Tan, et al.Identification of Isocitrate Dehydrogenase 1 as a potential diagnostic and prognostic biomarker for non-small cell lung cancer by proteomic analysis.《Molecular Cellular Proteomics》.2012,第11卷(第2期),第M111.008821-1-14页. * |
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