CN103175859A - Method for discriminating cold resistance of peony tissue - Google Patents
Method for discriminating cold resistance of peony tissue Download PDFInfo
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- CN103175859A CN103175859A CN201310089584XA CN201310089584A CN103175859A CN 103175859 A CN103175859 A CN 103175859A CN 201310089584X A CN201310089584X A CN 201310089584XA CN 201310089584 A CN201310089584 A CN 201310089584A CN 103175859 A CN103175859 A CN 103175859A
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Abstract
The invention belongs to the technical field of plant biology and specifically relates to a method for discriminating the cold resistance of a peony tissue. The method provided by the invention is capable of directly reflecting the adaptability and the buffering ability of the peony tissue to the low-temperature environment and the obvious degree of supercooling by measuring the freezing point temperature and the supercooling temperature of the peony plant tissue part and defining the difference between the freezing point temperature and the supercooling temperature as peony tissue supercooling ability (delta E). The greater the value of delta E is, the more obvious the supercooling phenomenon of the tissue is, the larger the temperature zone sensitive to low-temperature freezing injury is and the higher the adaptability of responding to a sudden low temperature is. The method provided by the invention is simple and convenient, and high in feasibility; and the method is capable of obtaining the supercooling point temperature and the freezing point temperature of the plant tissue from a temperature freezing curve, thereby providing convenient and accurate scientific basis for low-temperature safe storage and low-temperature freeze injury protection of peony.
Description
Technical field
The invention belongs to the plant biological field, be specifically related to the assay method that a kind of tree peony is organized cold tolerance.
Background technology
Freeze injury (cold injury) is a kind of of agrometeorological hazard, be that plant makes in tissue icing at the low temperature below 0 ℃, to the injury that plant is caused, the plant tissue inner cell is frozen and lose all physiological activities, finally cause the phenomenon of plant death or Mortality.The normal overwintering plant that has that occurs is organized freeze injury, freezing injury of fruit trees and commodity trees freeze injury etc., is the critical limitation factor that affects plant growth.The plant tissue cold tolerance be plant to the opposing of sub-zero temperature or restrain oneself ability, be the adaptive faculty to poor environment that plant forms in the long-term evolution process.The degree of hazard of plant depends on the speed of size, the length of duration and icing and the ice-melt of cooling extent.If after the plant tissue freeze injury, temperature raises suddenly and thaws too soon, plant just easily injures.The extreme low temperature weather that happens suddenly is in recent years produced the agriculture and forestry in the whole world and has all been caused serious harm.
Many weather scientists think both at home and abroad, environment wrecks and global warming causes extreme climate disaster in recent years again and again to appear at all over the world, nearest the have strong cold wave in Europe, Japanese severe snow and Chinese low temperature have just caused the arguement that " Little Ice Age " arrives, under the weather environment of this burst low temperature, cold tolerance and self-repairing capability to plant tissue put forward serious test, simultaneously the research of plant tissue cold tolerance are also paid attention to by Chinese scholars day by day.
There are some researches show under cold coercing, plant avoids tissue and freezes and adapt to low temperature environment by regulating self metabolism.When low ambient temperature reached the apparent freezing point temperature, the histoorgan cell began to freeze.Discharge the principle of the heat of condensation according to freezing, plant tissue Once you begin freezes, organize release heat that tissue temperature is raise, frozen temperature peak value occurs and jumps, this moment, most of ice crystal nucleus began to form, the heat release of histoorgan cell liquid and heat absorption are in equilibrium state, make plant tissue temperature change very within a period of time slow.The origin temp that peak value occurs is the supercooling temperature, and summit temperature is freezing temperature.
Domestic assay method about the plant cold resistance capability analysis still mainly concentrates on methods such as damaging to plants caused by sudden drop in temperature stage method, Determination of Physiological And Biochemical Indices at present.For example: with chlorophyll content, light and parameter (Pn, Tr, Gs, Ci), Proline, MDA, the variation of relative conductivity under the low temperature environment stress index that damages to plants caused by sudden drop in temperature as watermelon seedlings, inquire into the low temperature resistant physiological mechanism of seed water melon; Damage to plants caused by sudden drop in temperature index with conducts such as conductivity, soluble sugar, proline, relatively the cold resistance of different cultivars eggplant is strong and weak; The conductivity such as Hu Shanglian, soluble sugar content, soluble protein content, MDA, proline, superoxide dismutase, peroxidase etc. are as the index of bamboo chilling resistance power.These method complicated operations, the data error that analyzes are large, repeatable poor, affect the experimental result accuracy.
Summary of the invention
The purpose of this invention is to provide a kind of tree peony and organize the assay method of cold tolerance, not only simple to operate, quick, and also data are accurate, good reproducibility.
The present invention addresses the above problem the technical scheme of taking to be: a kind of tree peony is organized the assay method of cold tolerance, comprises the following steps:
A, draw materials: choose peony plant, the room temperature moisturizing is preserved, and is standby;
B, the peony plant in steps A is put into the polyethylene box, the peony plant surrounding is placed with absorbent cotton, to guarantee that in peony plant, temperature slowly reduces, makes in tissue gradually release heat and the phenomenon of freezing occurs;
C, the thermocouple temperature measurement instrument is connected on computers, a probe of thermocouple temperature measurement instrument is equipped with in the heat-preserving container of mixture of ice and water as inserting with reference to end, in the epidermal tissue of another root probe as peony plant in measuring junction inserting step B;
It is that the environment of 20 ℃ is preserved that D, the polyethylene box that peony plant will be housed are put into temperature, after the temperature in the epidermal tissue of peony plant reaches environment temperature, change rapidly the polyethylene box that peony plant is housed over to temperature in the environment of-30 ℃, with the temperature variation in the computer recording peony plant, and draw temperature variation curve;
E, obtain freezing point temperature and the supercooling temperature of peony plant according to the curve of record data and drafting, according to crossing cold energy power=freezing point temperature-supercooling temperature, calculate the cold energy power of crossing of each tissue site of peony plant.
Polyethylene box length described in step B and wall thickness are respectively 20cm, 20cm, 25cm, 20mm.
The invention has the beneficial effects as follows: the present invention is defined as tree peony by the difference with freezing point temperature and supercooling temperature and organized cold energy power (Δ E), can reflect more directly that the tree peony tissue adapts to and the size of surge capability and the obvious degree of supercooling phenomenon low temperature environment.The larger expression of Δ E value organizes that supercooling phenomenon is more obvious, that Cryogenic burns sensitive temperature district is reached more greatly the adaptive faculty of reply burst low temperature is stronger.Its beneficial effect comprises following three aspects:
Frozen temperature when the first, the present invention utilizes thermocouple temperature measurement instrument mensuration tree peony tissue temperature to reduce rapidly, method is easy, feasibility is high and can directly draw plant tissue undercooling point temperature and apparent freezing point temperature from the temperature freezing curve.And by data analysis and calculate tree peony and organized cold energy power and reversible damage temperature, and then definite tree peony is organized cold tolerance.
The second, the present invention is by arranging the rapid temperature-fall period of 20 ℃ to-30 ℃, in the time of can measuring and analyze tree peony and meet with extreme burst cooling to the adaptive faculty of low temperature environment.
Three, the present invention organizes supercooling point temperature, apparent freezing point temperature, crosses cold energy power and reversible damage temperature by analyzing tree peony, determines the size of tree peony cold tolerance.For tree peony low-temperature safety storage and low temperature freezing-disaster protection provide convenient, scientific basis accurately.
Description of drawings
Fig. 1 is the frozen temperature schematic diagram;
Fig. 2 is the white leaf temperature freezing curve figure of Varieties of Peony Feng Dan;
Fig. 3 is the Varieties of Peony red bud temperature freezing curve figure in Luoyang;
Fig. 4 is the Varieties of Peony red scale temperature freezing curve figure in Luoyang;
Fig. 5 is the petal temperature freezing curve figure of Varieties of Peony Hu Hong;
Fig. 6 is the red sepal temperature freezing curve figure of Varieties of Peony Shandong He;
Fig. 7 is the cold energy power of crossing of the red and white two kinds of Varieties of Peony different development stages of Feng Dan in Luoyang, Different Organs.
Embodiment
The below elaborates to embodiments of the invention: the present embodiment is implemented under take technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1: measure the red sepal temperature freezing curve of Varieties of Peony Shandong He
A kind of tree peony is organized the assay method of cold tolerance, comprises the following steps:
A. draw materials: choose respectively the Shandong He red peony plant that sprouting period, germination period, aobvious leaf phase, a leaf phase, leaf stretching period, windbell phase and Lu Se phase grow fine, the room temperature moisturizing is preserved, and prevents the peony plant dehydration, and is standby;
B. the peony plant in steps A is put into the polyethylene box, polyethylene box length and wall thickness are respectively 20cm, 20cm, 25cm, 20mm, the peony plant surrounding is placed with absorbent cotton, to guarantee that in peony plant, temperature slowly reduces, make in tissue gradually release heat and the phenomenon of freezing occurs;
C. the thermocouple temperature measurement instrument is connected on computers, a probe of thermocouple temperature measurement instrument is equipped with in the heat-preserving container of mixture of ice and water as inserting with reference to end, in the epidermal tissue of another root probe as peony plant in measuring junction inserting step B;
D., it is that the environment of 20 ℃ is preserved that the polyethylene box that peony plant will be housed is put into temperature, after the temperature in the epidermal tissue of peony plant reaches environment temperature, change rapidly the polyethylene box that peony plant is housed over to temperature in the environment of-30 ℃, with the temperature variation in the computer recording peony plant, and the drafting temperature variation curve, every each tissue site of peony plant is done respectively 15 repetitions;
E. obtain freezing point temperature and the supercooling temperature of peony plant according to the curve of record data and drafting, according to crossing cold energy power=freezing point temperature-supercooling temperature, calculate the cold energy power of crossing of each tissue site of peony plant.
result as shown in Figure 6, process by data and draw, the supercooling temperature is-11.09 ℃, freezing point temperature is-06.34 ℃, crossing cold energy power (Δ E) is 4.75 ℃, reversible semilethal temperature is-11.87 ℃, show that He red sepal in Shandong has obvious supercooling phenomenon, along with the reduction sepal of temperature is resisted low temperature by Self-regulation ability, when temperature is down to-11.09 ℃, the moment heat release due to rapid formation of ice-nucleus in tissue, in the tree peony body, temperature is increased to suddenly-6.34 ℃, this moment, the tree peony in-vivo tissue began to freeze, Self-regulation ability descends gradually, when temperature is down to-11.87 ℃, organize internal cause to freeze in a large number and the freeze injury phenomenon occurs, in body, temperature descends rapidly.
Embodiment 2: choose respectively the red and Feng Dan in Luoyang and test in vain
The University Of Science and Technology Of He'nan peony garden is all picked up from test material ' Luoyang is red ', ' Feng Dan is white ' in February, 2012 to April, collection and minute are followed successively by sprouting period, germination period, aobvious leaf phase, a leaf phase, leaf stretching period, windbell phase, reveal the look phase.Measure respectively the size of (old stem, scale, spire, bract, sepal, the petal) apparent freezing point of different parts in its rudiment process and supercooling point temperature, calculate different development stage peony organ cross cold energy power (Δ E) difference (℃).
Result as shown in Figure 7, the floral organ that Luoyang is red, Feng Dan is white same position is along with growing, it is crossed cold energy power and is reducing tendency.It is the most weak that rudiment later stage scale is crossed cold energy power, Luoyang red (1.04 ± 0.57 ℃), Feng Dan white (2.57 ± 1.80 ℃).At this moment many kinds of substance content incipient stage of relatively sprouting changes obviously in scale, and the cold-resistant machine-processed imperfection of resisting cold itself is tending towards degenerating, and the physiological action of protection floral organ inner tissue is lost substantially.
The floral organ different parts that Luoyang is red, Feng Dan is white is obvious in the cold energy power of the crossing difference of different budding stages.The performance of spire and bud (bract, sepal, petal) overall process exceeds cold energy power relatively, and their tissue development is perfect, and chilling resistance is stronger; And rudiment later stage scale it is crossed cold energy power and obviously is weaker than other position due to decline.
Claims (2)
1. a tree peony is organized the assay method of cold tolerance, it is characterized in that, comprises the following steps:
A, draw materials: choose peony plant, the room temperature moisturizing is preserved, and is standby;
B, the peony plant in steps A is put into the polyethylene box, the peony plant surrounding is placed with absorbent cotton;
C, the thermocouple temperature measurement instrument is connected on computers, a probe of thermocouple temperature measurement instrument is equipped with in the heat-preserving container of mixture of ice and water as inserting with reference to end, in the epidermal tissue of another root probe as peony plant in measuring junction inserting step B;
It is that the environment of 20 ℃ is preserved that D, the polyethylene box that peony plant will be housed are put into temperature, after the temperature in the epidermal tissue of peony plant reaches environment temperature, change rapidly the polyethylene box that peony plant is housed over to temperature in the environment of-30 ℃, with the in-house temperature variation of computer recording peony plant, and draw temperature variation curve;
E, obtain freezing point temperature and the supercooling temperature of peony plant tissue according to the curve of record data and drafting, according to crossing cold energy power=freezing point temperature-supercooling temperature, calculate the cold energy power of crossing of each tissue site of peony plant.
2. a kind of tree peony according to claim 1 is organized the assay method of cold tolerance, it is characterized in that: the polyethylene box length described in step B and wall thickness are respectively 20cm, 20cm, 25cm, 20mm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104372012A (en) * | 2014-11-06 | 2015-02-25 | 上海植物园 | Peony PseIF5A gene and application thereof |
| CN107290395A (en) * | 2017-06-08 | 2017-10-24 | 南京农业大学 | A kind of cold-resistant evaluation of lily and screening technique and the application of cold-resistant variety |
| CN111257364A (en) * | 2020-01-19 | 2020-06-09 | 河北省气象科学研究所 | Non-isolated plant organ supercooling point tester |
| CN113108947A (en) * | 2021-04-26 | 2021-07-13 | 南京农业大学 | Insect supercooling point computer nine-path measuring system, application program and measuring method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201451066U (en) * | 2009-08-04 | 2010-05-12 | 上海市绿化管理指导站 | Insect super-cooling point measuring instrument |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201451066U (en) * | 2009-08-04 | 2010-05-12 | 上海市绿化管理指导站 | Insect super-cooling point measuring instrument |
Non-Patent Citations (2)
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| 史国安等: "牡丹花器官结冰点和过冷却点的研究", 《园艺学报增刊》 * |
| 沈明泉等: "植物组织胞间和胞内过冷却点得简易测定法", 《植物生理学通讯》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104372012A (en) * | 2014-11-06 | 2015-02-25 | 上海植物园 | Peony PseIF5A gene and application thereof |
| CN107290395A (en) * | 2017-06-08 | 2017-10-24 | 南京农业大学 | A kind of cold-resistant evaluation of lily and screening technique and the application of cold-resistant variety |
| CN111257364A (en) * | 2020-01-19 | 2020-06-09 | 河北省气象科学研究所 | Non-isolated plant organ supercooling point tester |
| CN111257364B (en) * | 2020-01-19 | 2022-03-29 | 河北省气象科学研究所 | Non-isolated plant organ supercooling point tester |
| CN113108947A (en) * | 2021-04-26 | 2021-07-13 | 南京农业大学 | Insect supercooling point computer nine-path measuring system, application program and measuring method |
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Application publication date: 20130626 |