CN103172724A - Method for extracting ovomacroglobulin from egg white - Google Patents
Method for extracting ovomacroglobulin from egg white Download PDFInfo
- Publication number
- CN103172724A CN103172724A CN2013100734532A CN201310073453A CN103172724A CN 103172724 A CN103172724 A CN 103172724A CN 2013100734532 A CN2013100734532 A CN 2013100734532A CN 201310073453 A CN201310073453 A CN 201310073453A CN 103172724 A CN103172724 A CN 103172724A
- Authority
- CN
- China
- Prior art keywords
- gallus domesticus
- ovum gallus
- domesticus album
- ovomacroglobulin
- supernatant liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for extracting ovomacroglobulin from egg white. The method comprises a step of egg white solution preparation, a step of polyethylene glycol separating, a step of precipitate dissolving and processing, and a step of gel filtering chromatography purifying. The method for extracting ovomacroglobulin from egg white solves the problems comprising complex operation, long period and low yield of ovomacroglobulin separation and purification, and has the characteristics of simplicity, rapidness and high efficiency, the purity of the obtained ovomacroglobulin reaches 95.98%, the yield is greatly improved to 61.4% when the high purity is maintained, and only 10-12 hours are needed to complete the whole process of the method, so the method has the advantages of substantial operation simplification, cost reduction, and efficiency improvement.
Description
Technical field
The present invention relates to the extracting method of ovomacroglobulin, be specially a kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album.
Background technology
The Ovum Gallus domesticus album wide material sources, cheap, in dry-matter, protein content surpasses 80%, is one of optimal protein resource.Ovomacroglobulin is a kind of protein with important biomolecule activity in Ovum Gallus domesticus album, its content is only 0.5% of albumen protein, because of its biological activity with protease inhibition, show the functionally activies such as anti-inflammatory, promotion wound healing, inhibition septicemia, have a extensive future in fields such as medical science.
But because ovomacroglobulin content in Ovum Gallus domesticus album is low, and inappropriate preparation method will make its loss of activity, and therefore how purifying obtains pure protein becomes one of difficult problem of its research.At present, the separation and purification of ovomacroglobulin all obtains by the method for multistep chromatography, and step is various, consuming time very long, and the use of multiple filler has increased production cost greatly.In addition, in sepn process, the operation of each step all will inevitably cause target protein loss in various degree, because operation steps is various, cause the product yield very low, be only 20% left and right.Therefore, develop a kind of simple to operate, the purifying cycle is short, yield is high ovomacroglobulin separation purification method, will have important using value.
Summary of the invention
The objective of the invention is in order to overcome defects, a kind of simple, quick and efficient method of extracting ovomacroglobulin from Ovum Gallus domesticus album is provided.
Above-mentioned purpose is achieved through the following technical solutions:
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Adopt Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 0.6～3 times of volume in the Ovum Gallus domesticus album, add complete after, continue to stir 2～3h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Accurately take above-mentioned Ovum Gallus domesticus album solution, add 3～5% PEG 8000 by quality, stir 2～3h, then centrifugal 8～15min under the rotating speed of 8000～12000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 8～10%, stir 2～3h, centrifugal 8～15min under the rotating speed of 8000～12000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.3～0.5M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/8～1/5, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that the 3rd step was obtained is injected in chromatography column, carries out wash-out with the PBS damping fluid, collect first chromatographic peak, namely get ovomacroglobulin.
Preferably, the add-on of distilled water described in step (1) is 1 times of Ovum Gallus domesticus album volume.
Preferably, the PEG 8000 that adds for the first time in step (2) is 3.2～4.4% of Ovum Gallus domesticus album solution quality, and the mass concentration of the PEG 8000 that adds for the second time in supernatant liquor is 8.4～9.2%.
Further preferably, the PEG 8000 that adds for the first time in step (2) is 4% of Ovum Gallus domesticus album solution quality, and the mass concentration of the PEG 8000 that adds for the second time in supernatant liquor is 8.8%.
Preferably, the elution flow rate in step (4) is 0.5～0.8mL/min.
Further preferably, the elution flow rate in step (4) is 0.65～0.75mL/min.
Beneficial effect of the present invention is:
(1) according to the extraction scheme of the characteristics of egg white powder matter design ovomacroglobulin: at first according to the characteristics of ovomucin poor solubility, the employing polyethylene glycol precipitation is removed, and the greatest differences on recycling ovomacroglobulin and other albumen protein molecular weight adopts gel permeation chromatography to be purified.Only use a step chromatography in process, greatly simplified operation, also reduced the cost of buying chromatographic stuffing simultaneously.
(2) whole procedure only needed to complete in 10～12 hours, compared with the existing method cycle of 2～3 days, had greatly shortened the time, had improved efficient, had reduced artificial and time cost.
(3) gained ovomacroglobulin sample purity reaches 95.98%, is keeping the highly purified while, and the rate of recovery also significantly improves, and the rate of recovery approximately 20% is increased to 61.4% from now methodical, takes full advantage of the albumen protein resource.
Description of drawings
Fig. 1 is ovomacroglobulin separation and purification schema;
Fig. 2 is the gel permeation chromatography collection of illustrative plates;
Fig. 3 is ovomacroglobulin SDS-PAGE electrophorogram;
Fig. 4 is the high phase liquid chromatogram of ovomacroglobulin.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get the Fresh Egg of output on the same day, manually beat eggs, use separator with egg white and yolk separately, collect Ovum Gallus domesticus album, magnetic agitation limit, limit slowly adds isopyknic pure water in Ovum Gallus domesticus album, add complete after, continue magnetic agitation 3h, get Ovum Gallus domesticus album solution.
(2) polyoxyethylene glycol separates
Accurately take above-mentioned Ovum Gallus domesticus album solution, add 4% PEG 8000 by quality, fully stir 3h, then centrifugal 15min under the rotating speed of 10000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 8.8%, as front stirring, centrifugal again, collecting precipitation.
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.4M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/7, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and wait to be further purified.
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, chromatography column (uses the PBS damping fluid to carry out balance to chromatography column after 16 * 600mm) fillings.Get the supernatant liquor 3mL that obtains in step (3) and be injected in chromatography column, use the PBS damping fluid to carry out wash-out with the flow velocity of 0.65～0.75mL/min, the chromatography collection of illustrative plates is collected first chromatographic peak as shown in Figure 2, namely gets ovomacroglobulin.
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 0.6 times of volume in the Ovum Gallus domesticus album, add complete after, continue to stir 2h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Take above-mentioned Ovum Gallus domesticus album solution, add 3% PEG 8000 by quality, stir 2h, then centrifugal 8min under the rotating speed of 12000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 8%, stir 2h, centrifugal 8min under the rotating speed of 12000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.3M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/8, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that obtains in step (3) is injected in chromatography column, carry out wash-out with the PBS damping fluid with the flow velocity of 0.5～0.8mL/min, collect first chromatographic peak, namely get ovomacroglobulin.
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 3 times of volumes in the Ovum Gallus domesticus album, add complete after, continue to stir 2h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Take above-mentioned Ovum Gallus domesticus album solution, add 5% PEG 8000 by quality, stir 2h, then centrifugal 15min under the rotating speed of 8000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 10%, stir 2h, centrifugal 15min under the rotating speed of 8000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.5M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/5, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that obtains in step (3) is injected in chromatography column, carry out wash-out with the PBS damping fluid, collect first chromatographic peak, namely get ovomacroglobulin.
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 2 times of volumes in the Ovum Gallus domesticus album, add complete after, continue to stir 2.5h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Take above-mentioned Ovum Gallus domesticus album solution, add 3.2% PEG 8000 by quality, stir 2.5h, then centrifugal 15min under the rotating speed of 8000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 8.4%, stir 2.5h, centrifugal 8min under the rotating speed of 12000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.5M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/6, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that obtains in step (3) is injected in chromatography column, carry out wash-out with the PBS damping fluid, collect first chromatographic peak, namely get ovomacroglobulin.
Embodiment 5
A kind of method of extracting ovomacroglobulin from Ovum Gallus domesticus album, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 1 times of volume in the Ovum Gallus domesticus album, add complete after, continue to stir 2.5h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Take above-mentioned Ovum Gallus domesticus album solution, add 4.4% PEG 8000 by quality, stir 2h, then centrifugal 15min under the rotating speed of 12000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 9.2%, stir 3h, centrifugal 8min under the rotating speed of 8000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.3M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/6, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that obtains in step (3) is injected in chromatography column, carry out wash-out with the PBS damping fluid, collect first chromatographic peak, namely get ovomacroglobulin.
Embodiment 6 methods are identified
(1) poly-propionic acid amide gel electrophoresis is identified
Get ovomacroglobulin sample solution 80 microlitres that chromatography obtains, the albumen sample-loading buffer (5 times of concentration) that adds 20 microlitres, after mixing, boiling water bath heating is approximately 5 minutes, and getting 5 microlitres after cooling carries out conventional SDS-PAGE electrophoresis, resolving gel concentration is 8%, constant voltage 100V.Electrophoresis result is (swimming lane 1 is protein standard molecular weight mark, and 2～4 is ovomacroglobulin) as shown in Figure 3, and sample is shown as single band on gel, illustrate that its purity is higher, uses its molecular weight of computed in software, is about 175kDa, and is consistent with bibliographical information.
(2) purity testing
Adopt high phase liquid chromatography for measuring, use G2000SW
XLMolecular-exclusion chromatography post (7.8x300mm) is analyzed, and liquid phase systems is Waters2695, and the detection wavelength is 280nm, and moving phase is that (0.1mol/L, pH7.0 contain 0.1mol/L Na to phosphate buffered saline buffer
2SO
4), flow velocity is 0.8ml/min.The chromatography collection of illustrative plates carries out integration by software to peak area as shown in Figure 4, according to peak area, sample purity is carried out quantitatively, and the purity of calculating embodiment 1～5 is respectively 96.32%, 95.84%, 96.01%, 96.20%, 95.55%, average out to 95.98%.
(3) rate of recovery is calculated
According to quality, protein content and the purity of purifying gained ovomacroglobulin from Ovum Gallus domesticus album, use following formula to calculate the rate of recovery of each protein of gained.
In embodiment 1, the 1000g Ovum Gallus domesticus album separates the cumulative volume 394.4mL that obtains the ovomacroglobulin sample, and sample protein content is 0.82mg/mL, and purity of protein is 96.32%, and therefore calculating and obtaining pure ovomacroglobulin is 311.5mg.In egg white, the protein theoretical content is 10.2%, and ovomacroglobulin accounts for albumen protein 0.5%, so in 1000g egg white, total ovomacroglobulin is 510mg, and can be calculated the rate of recovery is 61.1%.
Calculate after the same method embodiment 2,3,4,5 the rate of recovery, result is respectively 58.7%, 63.4%, 61.5%, 62.3%, and the average recovery rate of embodiment 1～5 is 61.4%.
Claims (6)
1. a method of extracting ovomacroglobulin from Ovum Gallus domesticus album, is characterized in that, the method comprises the following steps:
(1) preparation of Ovum Gallus domesticus album solution
Get Fresh Egg, with egg white and yolk separately, collect Ovum Gallus domesticus album, slowly add while stirring the distilled water of 0.6 ~ 3 times of volume in the Ovum Gallus domesticus album, add complete after, continue to stir 2 ~ 3h, get Ovum Gallus domesticus album solution;
(2) polyoxyethylene glycol separates
Take above-mentioned Ovum Gallus domesticus album solution, add 3 ~ 5% PEG 8000 by quality, stir 2 ~ 3h, then centrifugal 8 ~ 15min under the rotating speed of 8000 ~ 12000 rev/mins, collect supernatant liquor, then add PEG 8000 in supernatant liquor, make the mass concentration of PEG 8000 in supernatant liquor reach 8 ~ 10%, stir 2 ~ 3h, centrifugal 8 ~ 15min under the rotating speed of 8000 ~ 12000 rev/mins then, collecting precipitation;
(3) resolution of precipitate and processing
Gained precipitation is placed in the PBS damping fluid that contains 0.3 ~ 0.5M sodium-chlor, the volume of PBS damping fluid be in step (1) the Ovum Gallus domesticus album volume 1/8 ~ 1/5, stir and make resolution of precipitate, more centrifugally remove a small amount of insolubles, get supernatant liquor and treat next step purifying;
(4) gel permeation chromatography purifying
Use Sephacryl S-200 HR as the gel permeation chromatography filler, with the PBS damping fluid, chromatography column is carried out balance after filling, the supernatant liquor that obtains in step (3) is injected in chromatography column, carry out wash-out with the PBS damping fluid, collect first chromatographic peak, namely get ovomacroglobulin.
2. the method for extracting ovomacroglobulin from Ovum Gallus domesticus album as claimed in claim 1, it is characterized in that: the add-on of distilled water described in step (1) is 1 times of Ovum Gallus domesticus album volume.
3. the method for extracting ovomacroglobulin from Ovum Gallus domesticus album as claimed in claim 1 or 2, it is characterized in that: the PEG 8000 that adds for the first time in step (2) is 3.2 ~ 4.4% of Ovum Gallus domesticus album solution quality, and the mass concentration of the PEG 8000 that adds for the second time in supernatant liquor is 8.4 ~ 9.2%.
4. the method for extracting ovomacroglobulin from Ovum Gallus domesticus album as claimed in claim 3, it is characterized in that: the PEG 8000 that adds for the first time in step (2) is 4% of Ovum Gallus domesticus album solution quality, and the mass concentration of the PEG 8000 that adds for the second time in supernatant liquor is 8.8%.
5. the method for extracting ovomacroglobulin from Ovum Gallus domesticus album as claimed in claim 1, it is characterized in that: the elution flow rate in step (4) is 0.5 ~ 0.8mL/min.
6. the method for extracting ovomacroglobulin from Ovum Gallus domesticus album as claimed in claim 5, it is characterized in that: the elution flow rate in step (4) is 0.65 ~ 0.75mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310073453.2A CN103172724B (en) | 2013-03-08 | 2013-03-08 | Method for extracting ovomacroglobulin from egg white |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310073453.2A CN103172724B (en) | 2013-03-08 | 2013-03-08 | Method for extracting ovomacroglobulin from egg white |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103172724A true CN103172724A (en) | 2013-06-26 |
| CN103172724B CN103172724B (en) | 2014-06-25 |
Family
ID=48632971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310073453.2A Active CN103172724B (en) | 2013-03-08 | 2013-03-08 | Method for extracting ovomacroglobulin from egg white |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103172724B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106496318A (en) * | 2016-12-01 | 2017-03-15 | 成都大学 | A kind of method of combined extracting CLU and ovomacroglobulin from fowls albumen |
| WO2017045617A1 (en) * | 2015-09-17 | 2017-03-23 | 中国人民解放军军事医学科学院野战输血研究所 | Method for separating and purifying α2-macroglobulin from sediment of cohn component iv |
| CN108088727A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | The preprocess method of protein in a kind of in vitro body fluid |
| CN108640986A (en) * | 2018-05-23 | 2018-10-12 | 遵义医学院 | A kind of egg white anaphylactogen separation method |
| CN109206506A (en) * | 2018-03-16 | 2019-01-15 | 王小虎 | The preparation of modified ultrafiltration centrifugal process is rich in the device and method of alpha2 Macroglobulin serum |
| CN110063926A (en) * | 2019-05-23 | 2019-07-30 | 余晨 | A kind of preparation method of composite micro-capsule anti UV finishing agent |
| CN113754758A (en) * | 2021-10-25 | 2021-12-07 | 上海乐合生物科技有限公司 | Method for purifying lipoprotein a |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604915A (en) * | 2012-03-27 | 2012-07-25 | 华中农业大学 | Method for jointly extracting a variety of proteins from egg white |
-
2013
- 2013-03-08 CN CN201310073453.2A patent/CN103172724B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604915A (en) * | 2012-03-27 | 2012-07-25 | 华中农业大学 | Method for jointly extracting a variety of proteins from egg white |
Non-Patent Citations (1)
| Title |
|---|
| 耿放等: "卵巨球蛋白结构与功能特性研究进展", 《第九届中国蛋品科技大会论文集》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017045617A1 (en) * | 2015-09-17 | 2017-03-23 | 中国人民解放军军事医学科学院野战输血研究所 | Method for separating and purifying α2-macroglobulin from sediment of cohn component iv |
| CN108088727A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | The preprocess method of protein in a kind of in vitro body fluid |
| CN108088727B (en) * | 2016-11-21 | 2020-07-07 | 中国科学院大连化学物理研究所 | Pretreatment method of protein in-vitro body fluid |
| US11365214B2 (en) | 2016-11-21 | 2022-06-21 | Dalian Institute Of Chemical Physics, Chinese Academy Of Sciences | Method for pretreating protein in ex vivo body fluid |
| CN106496318A (en) * | 2016-12-01 | 2017-03-15 | 成都大学 | A kind of method of combined extracting CLU and ovomacroglobulin from fowls albumen |
| CN106496318B (en) * | 2016-12-01 | 2019-10-01 | 成都大学 | A method of combined extracting cluster protein and ovomacroglobulin from fowls albumen |
| CN109206506A (en) * | 2018-03-16 | 2019-01-15 | 王小虎 | The preparation of modified ultrafiltration centrifugal process is rich in the device and method of alpha2 Macroglobulin serum |
| CN108640986A (en) * | 2018-05-23 | 2018-10-12 | 遵义医学院 | A kind of egg white anaphylactogen separation method |
| CN110063926A (en) * | 2019-05-23 | 2019-07-30 | 余晨 | A kind of preparation method of composite micro-capsule anti UV finishing agent |
| CN113754758A (en) * | 2021-10-25 | 2021-12-07 | 上海乐合生物科技有限公司 | Method for purifying lipoprotein a |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103172724B (en) | 2014-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103172724B (en) | Method for extracting ovomacroglobulin from egg white | |
| CN102604915B (en) | Method for jointly extracting a variety of proteins from egg white | |
| CN102952187B (en) | Preparation method of high-purity bovine serum albumin | |
| CN106478810B (en) | A kind of method of phycocyanin and allophycocyanin while that isolate and purify SILVER REAGENT | |
| CN105017412A (en) | Method for separating high-purity bovine serum albumin from bovine serum | |
| CN101857635A (en) | Continuous separation method for three proteins in bovine plasma | |
| CN103804480A (en) | Preparation method of shrimp tropomyosin allergen primary standard substance | |
| CN104356227A (en) | Efficient method for concentration and extraction of lipids, lipoprotein and apolipoprotein in serum | |
| Nicholas et al. | Continuous chromatography apparatus: III. Application | |
| CN103645328A (en) | Method for preparing peanut allergic protein Arah2 standard | |
| CN102796193A (en) | Method for extracting ovotransferrin from egg white | |
| CN108640342B (en) | Method for separating whey protein from whey wastewater by foam separation method | |
| CN103833840A (en) | Method for extracting high-density lipoprotein and separating and purifying apolipoprotein apoA-I from human plasma | |
| CN105241728A (en) | Preparation method of human glycated hemoglobin | |
| CN104897835A (en) | Method for rapidly determining oligoguluronic acid by employing UPLC-Q-TOF/MS (ultra-high performance liquid chromatography-quadrupole rods tandem time-of-flight/mass spectrometry) technology | |
| CN103103170B (en) | Production process for cow or sheep hyaluronidase | |
| CN1243015C (en) | Separating and purifying technology for micro algae phycoerythrin | |
| CN103524342B (en) | Adopt the method for adjustable ternary solvent system sharp separation tussilagone | |
| CN102109431A (en) | Method for preparing shrimp allergen standard substance | |
| CN109762061A (en) | A kind of purification process for the monoclonal antibody being simple and efficient | |
| CN105061630A (en) | Method for extracting and purifying gentiana polysaccharide | |
| CN101045750A (en) | Extraction process of camel colostrum immune globulin IgA, IgG. | |
| CN102796190A (en) | Method for extracting riboflavin binding protein from egg white | |
| CN102749238A (en) | Blood station negative-filtered leukocyte eluting and fixing method and dedicated filter therefor | |
| CN103278370A (en) | Method sutiable for two-dimensional electrophoresis extraction and separation of total protein of rat and mouse testicular tissue sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |