CN103162770A - Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine - Google Patents
Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine Download PDFInfo
- Publication number
- CN103162770A CN103162770A CN201310112860XA CN201310112860A CN103162770A CN 103162770 A CN103162770 A CN 103162770A CN 201310112860X A CN201310112860X A CN 201310112860XA CN 201310112860 A CN201310112860 A CN 201310112860A CN 103162770 A CN103162770 A CN 103162770A
- Authority
- CN
- China
- Prior art keywords
- liquid level
- sampling probe
- immunohistochemical staining
- base plate
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to a method of trace reagent liquid level detection for an immunohistochemical staining machine. The method of the trace reagent liquid level detection for the immunohistochemical staining machine comprises the following steps: enabling the needle head of a sampling needle to be electrically insulated with surrounding structures, enabling a baseboard to be electrically insulated with an engine base to be laid between a reagent bottle and the engine base with the fact that the baseboard and the needle head of the sampling needle are conductors, judging whether capacitance between the needle head of the sampling needle and the baseboard is effectively stepped, and if the answer is yes, determining whether the sampling needle contacts with the liquid level. The invention further relates to a device of the trace reagent liquid level detection which comprises a capacitor and a detection circuit. Two poles of the capacitor are formed by the needle head of the sampling needle and the baseboard, the needle head of the sampling needle is a conductor, and the needle head of the sampling needle is electrically insulated with the surrounding structures. The baseboard is laid between a reagent bottle and an engine base, and the baseboard is a conductor and is electrically insulated with the engine base. The detection circuit comprises a capacitance converter and a microprocessor. The method and the device of the trace reagent liquid level detection are capable of detecting remaining amount of trace reagents in the reagent bottle, and are high in detection accuracy, simple in structure, and low in cost.
Description
Technical field
The present invention relates to a kind of reagent liquid level sensor, particularly a kind of trace reagent liquid level checking method and device for the immunohistochemical staining machine.
Background technology
Expression is an important colouring method in pathology technique, is used widely in pathology work, is one of Pathology Doctors ' important supplementary means of disease being made diagnosis.Immunohistochemical staining is the pathology technique that development in recent years is got up, operation itself is easy to be subject to the impact of humidity, temperature, time and human factor, cause result stable not, can not carry out well the result contrast, quality control is more difficult assurance also, therefore pathology technique person is had higher requirement.
Owing to being subject to the expensive impact of import instrument, present hospital more than 99% is manual operations at home; And the main problem that exists of traditional manual operations is: 1, be easy to be subject to the impact of uncertain factor, cause occurring unnecessary mistaken diagnosis phenomenon in pathological diagnosis; 2, consuming time: dyeing each time needs more than 4-5 consuming time hour; 3, easily make mistakes: add or forget and add because the antibody used of each " immunohistochemical staining " and reagent type reach the tens of kinds of easy mistakes of artificial reagent adding, the reaction time can not effectively be controlled; 4, can not accomplish standardization and standardized laboratory room managing.
In recent years, along with clinical attention to pathological diagnosis, the pathology workload increases year by year, and the application of pathology technique also has a great development thereupon, and expression has become indispensable means in the pathological diagnosis.Compare with traditional manual operations, the superiority of full-automatic immune tissue chemical dyeing instrument is apparently higher than the former, and therefore, the application of full-automatic immuning tissue staining technique has received increasing concern.
Full-automatic immune tissue chemical dyeing instrument is to draw reagent with elongated sampling probe, is put in a test tube and tests.In this process, need the strict amount of controlling sample and reagent.Uptake how accurately to control sample and reagent is a difficult problem in full-automatic immune tissue chemical dyeing instrument always, and the method for internal and international upper use at present roughly is divided into following two kinds:
L, screw type
The method is that the piston with syringe is connected with the nut of leading screw, drives leading screw by stepper motor and rotates.Control by the step number of control step motor and get liquid measure.But this method can't be surveyed liquid level, sampling probe must be deep into bottom reagent bottle, just begins sampling.This mode one side sampling probe appearance has sample or reagent is residual, cause the waste of sample or reagent, and the difficulty of sampling probe is cleaned; And because the amount itself of the sample of drawing and reagent is fewer, generally only have 50～60 μ L, the sample that the sampling probe appearance is residual or reagent will affect measuring accuracy.On the other hand, do not know whether also have enough amount of reagent in reagent bottle during sampling.
2, capacitance method
This method as capacitive transducer, is converted into the change of electric capacity by potential comparator the variation of frequency with the sampling probe inner and outer pipes, with square wave output, by the variation of a single-chip microcomputer calculated rate.This method can detect liquid level and follow the tracks of the liquid level variation, but in primary antibodie, two absorptions that resist,, leaves in a very little reagent bottle less than 25ml due to every money primary antibodie, two amount of reagent that resist.Place on dyeing instrument and reach nearly 50 reagent bottles, bottleneck is little, is difficult to detect the surplus of reagent in reagent bottle with general way.And existing scheme detection sensitivity is not high, and hysteresis quality is arranged, and can't detect when liquid is few, and cost is relatively high.
Summary of the invention
The technical problem to be solved in the present invention is: not high in order to overcome in prior art in reagent bottle the surplus accuracy of detection of trace reagent, the deficiency that can't detect when even amount is few the invention provides a kind of trace reagent liquid level checking method and device for the immunohistochemical staining machine.
The technical solution adopted for the present invention to solve the technical problems is: a kind of trace reagent liquid level sensor for the immunohistochemical staining machine, described immunohistochemical staining machine comprises sampling probe and support, be placed with reagent bottle on described support, the trace reagent liquid level sensor comprises electric capacity and testing circuit; The two poles of the earth of described electric capacity are comprised of syringe needle and the base plate of sampling probe, and the syringe needle of described sampling probe is conductor, and with the surrounding structure electric insulation; Described base plate paving is between reagent bottle and support, and described base plate is conductor, and by insulcrete and support electric insulation.
Described testing circuit comprise microprocessor and the capacitive path that consists of for detection of the syringe needle of described sampling probe and described base plate on the capacity commutator of micro-alternating current flow valuve, the signal input part of described microprocessor is connected with the signal output part of capacity commutator.Due to the wide ranges of current detecting, can not be confined to certain scope, therefore, detection sensitivity of the present invention is high, also can detect when liquid is few.
Described capacity commutator is AD7151, and the signal input part of described microprocessor is connected with the serial ports of AD7151.
In order to make sampling probe and surrounding structure electric insulation, syringe needle one end of described sampling probe is suction end, and other end cover has the liquid pipe, and described liquid pipe is insulating material.
A kind of trace reagent liquid level checking method for the immunohistochemical staining machine comprises the following steps:
(a) measuring the syringe needle of sampling probe and the electric capacity between base plate, and constantly read capacitance, judge whether capacitance effective transition occurs, is to determine that sampling probe touches liquid level.Wide ranges due to current detecting, so sensing range is also wide, the amount volume can effectively detect less, in step (a), micro-alternating current flow valuve on the capacitive path that syringe needle by detecting described sampling probe and described base plate consist of obtains the syringe needle of sampling probe and the capacitance between base plate.
(b) when effective transition occurs in the capacitance between the syringe needle that sampling probe detected and base plate, record the height that sampling probe descends, then according to the shape and the height that the sampling probe that records descends of reagent bottle, calculate the residue amount of reagent in reagent bottle.
In step (a), syringe needle and the capacitance between base plate of sampling probe carried out analog to digital conversion, constantly read described capacitance after analog to digital conversion by software; The described capacitance of described software after to analog to digital conversion analyzed judgement and extracted effective value; By described software, described effective value is carried out digital filtering, judge whether filtered described effective value effective transition occurs, and is to determine that sampling probe touches liquid level.
Principle of work of the present invention is as follows: the syringe needle of sampling probe is an electrode of electric capacity, and base plate is another electrode, and reagent bottle is insulating polymeric material, measures the syringe needle of sampling probe and the electric capacity between base plate; When the syringe needle of sampling probe did not touch reagent, capacitance was stabilized on a smaller value; When the sampling probe slow decreasing, when touching liquid level, because the reagent in reagent bottle has weak inductive, transition will occur in electric capacity, for fear of unnecessary erroneous judgement, improve accuracy of detection, can analyze judgement to the capacitance of capacity commutator output, extract effective value, and carry out digital filtering, judge whether capacitance effective transition occurs, thereby determine whether sampling probe touches liquid level.Effective transition described here is the transition that causes in order to be different from interference, just must can not draw according to test.Through so processing, system is less demanding to electric environment for use, and antijamming capability is strong, must not debug according to site environment again.Draw liquid level position according to effective transition of electric capacity, namely after liquid level, can calculate the residue amount of reagent according to the shape of reagent bottle, for systematic analysis, demonstration etc.
The invention has the beneficial effects as follows that trace reagent liquid level checking method and device for the immunohistochemical staining machine of the present invention can detect the surplus of trace reagent in reagent bottle, accuracy of detection is high, there is no hysteresis quality, also can detect when liquid is few, apparatus structure is simple, and cost is lower.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is the trace reagent liquid level checking method for the immunohistochemical staining machine of the present invention and the fundamental diagram of device.
Fig. 2 is the structural representation of the optimum embodiment of the trace reagent liquid level sensor for the immunohistochemical staining machine of the present invention.
Fig. 3 is the partial enlarged drawing at A place in Fig. 2.
In figure 1, support, 2, reagent bottle, 3, base plate, 4, insulcrete, 5, drip the oxygen needle tubing, 6, chuck, 7, the guiding pressing plate, 8, hexagonal axis, 9, oilless bearing, 10, pinion and rack, 11, the liquid pipe.
Embodiment
In conjunction with the accompanying drawings, the present invention is further detailed explanation.These accompanying drawings are the schematic diagram of simplification, basic structure of the present invention only is described in a schematic way, so it only show the formation relevant with the present invention.
As shown in Figure 2 and Figure 3, the structural representation of the optimum embodiment of the trace reagent liquid level sensor for the immunohistochemical staining machine of the present invention.The immunohistochemical staining machine comprises sampling probe and support 1, is placed with reagent bottle 2 on support 1, and the trace reagent liquid level sensor comprises electric capacity and testing circuit; The two poles of the earth of electric capacity are comprised of syringe needle and the base plate 3 of sampling probe, and the syringe needle of sampling probe is conductor, and with the surrounding structure electric insulation; Base plate 1 is laid between reagent bottle 2 and support 1, and base plate 1 is sheet metal, and by insulcrete 4 and support 1 electric insulation, the common reagent bottle holder that forms of base plate 1 and insulcrete 4; Testing circuit comprises capacity commutator AD7151 and microprocessor, and the signal input part of microprocessor is connected with the serial ports of AD7151, has CDC circuit (electric capacity-digital quantizer) in AD7151.
In the present embodiment, reagent bottle 2 is insulating polymeric material, sampling probe comprises an oxygen needle tubing 5, chuck 6, guiding pressing plate 7, oilless bearing 9, pinion and rack 10 and liquid pipe 11, drip oxygen needle tubing 5 as the syringe needle of sampling probe, one end is as suction end, one end is connected with liquid pipe 11, and liquid pipe 11 is placed in and drips outside oxygen needle tubing 5, and liquid pipe 11 is rubber insulation material; Pinion and rack 10 is placed in outside liquid pipe 11, and chuck 6 is used for fixing the connecting portion that drips oxygen needle tubing 5 and liquid pipe 11, and liquid pipe 11 and the close end that drips oxygen needle tubing 5 of the connecting portion of pinion and rack 10.Dripping oxygen needle tubing 5 is the metal needle tubing.
As Figure 1-3, a kind of trace reagent liquid level checking method for the immunohistochemical staining machine of the present invention comprises the following steps:
After the initialization of CDC circuit, micro-alternating current flow valuve on syringe needle by measuring sampling probe and the capacitive path of base plate formation, obtain the syringe needle of sampling probe and the capacitance between base plate, microprocessor constantly reads the capacitance of capacity commutator output, capacitance is analyzed judgement, extract effective value, and carry out the single order digital filtering, judging whether capacitance effective transition occurs, is to determine that sampling probe touches liquid level.
When effective transition occurs in the capacitance between the syringe needle that sampling probe detected and base plate 3, record the height that sampling probe descends, then according to the shape and the height that the sampling probe that records descends of reagent bottle 2, calculate the residue amount of reagent in reagent bottle 2, for systematic analysis, demonstration etc.
Take above-mentioned foundation desirable embodiment of the present invention as enlightenment, by above-mentioned description, the relevant staff can in the scope that does not depart from this invention technological thought, carry out various change and modification fully.The technical scope of this invention is not limited to the content on instructions, must determine its technical scope according to the claim scope.
Claims (7)
1. trace reagent liquid level sensor that is used for the immunohistochemical staining machine, described immunohistochemical staining machine comprises sampling probe and support (1), is placed with reagent bottle (2) on described support (1), it is characterized in that: comprise electric capacity and testing circuit; The two poles of the earth of described electric capacity are comprised of syringe needle and the base plate (3) of sampling probe, and the syringe needle of described sampling probe is conductor, and with the surrounding structure electric insulation; Described base plate (1) is laid between reagent bottle (2) and support (1), and described base plate (1) is conductor, and by insulcrete (4) and support (1) electric insulation.
2. the trace reagent liquid level sensor for the immunohistochemical staining machine as claimed in claim 1, it is characterized in that: described testing circuit comprise microprocessor and the capacitive path that consists of for detection of the syringe needle of described sampling probe and described base plate on the capacity commutator of micro-alternating current flow valuve, the signal input part of described microprocessor is connected with the signal output part of capacity commutator.
3. the trace reagent liquid level sensor for the immunohistochemical staining machine as claimed in claim 2, it is characterized in that: described capacity commutator is AD7151, the signal input part of described microprocessor is connected with the serial ports of AD7151.
4. the trace reagent liquid level sensor for the immunohistochemical staining machine as described in any one in claim 1-3, it is characterized in that: syringe needle one end of described sampling probe is suction end, and other end cover has liquid pipe (11), and described liquid pipe (11) is insulating material.
5. the trace reagent liquid level checking method of the trace reagent liquid level sensor for the immunohistochemical staining machine as described in any one in claim 1-4 is characterized in that comprising the following steps:
(a) measuring the syringe needle of sampling probe and the electric capacity between base plate, and constantly read capacitance, judge whether capacitance effective transition occurs, is to determine that sampling probe touches liquid level; In step (a), the micro-alternating current flow valuve on the capacitive path that the syringe needle by detecting described sampling probe and described base plate consist of obtains the syringe needle of sampling probe and the capacitance between base plate (3).
6. the trace reagent liquid level checking method for the immunohistochemical staining machine as claimed in claim 5, it is characterized in that: also comprise step (b), when effective transition occurs in the capacitance between the syringe needle that sampling probe detected and base plate (3), record the height that sampling probe descends, then according to the shape and the height that the sampling probe that records descends of reagent bottle (2), calculate the residue amount of reagent in reagent bottle (2).
7. the trace reagent liquid level checking method for the immunohistochemical staining machine as claimed in claim 5, it is characterized in that: in step (a), the syringe needle of sampling probe and the capacitance between base plate (3) are carried out analog to digital conversion, constantly read described capacitance after analog to digital conversion by software; The described capacitance of described software after to analog to digital conversion analyzed judgement and extracted effective value; By described software, described effective value is carried out digital filtering, judge whether filtered described effective value effective transition occurs, and is to determine that sampling probe touches liquid level.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112860.XA CN103162770B (en) | 2013-04-02 | 2013-04-02 | Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112860.XA CN103162770B (en) | 2013-04-02 | 2013-04-02 | Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103162770A true CN103162770A (en) | 2013-06-19 |
| CN103162770B CN103162770B (en) | 2017-02-22 |
Family
ID=48586045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310112860.XA Expired - Fee Related CN103162770B (en) | 2013-04-02 | 2013-04-02 | Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103162770B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108981849A (en) * | 2018-07-26 | 2018-12-11 | 宁波海壹生物科技有限公司 | A kind of fluid level detection system and method for Medical Instruments |
| CN110146141A (en) * | 2019-04-26 | 2019-08-20 | 浙江大学 | Improve the liquid detection device and method of socket type sample needle |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2927005Y (en) * | 2006-06-13 | 2007-07-25 | 姚冬 | Liquid-level detection circuit device |
| JP2007322288A (en) * | 2006-06-01 | 2007-12-13 | Olympus Corp | Liquid level detector |
| CN201476844U (en) * | 2009-08-10 | 2010-05-19 | 苏州捷美电子有限公司 | Capacitive liquid-level detecting device |
| CN102288260A (en) * | 2011-07-06 | 2011-12-21 | 长春迪瑞医疗科技股份有限公司 | Device and method for remaining amount of reagent |
| CN203148516U (en) * | 2013-04-02 | 2013-08-21 | 常州市微凯医疗科技有限公司 | Trace reagent liquid level detecting device for immunohistochemical stainer |
-
2013
- 2013-04-02 CN CN201310112860.XA patent/CN103162770B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007322288A (en) * | 2006-06-01 | 2007-12-13 | Olympus Corp | Liquid level detector |
| CN2927005Y (en) * | 2006-06-13 | 2007-07-25 | 姚冬 | Liquid-level detection circuit device |
| CN201476844U (en) * | 2009-08-10 | 2010-05-19 | 苏州捷美电子有限公司 | Capacitive liquid-level detecting device |
| CN102288260A (en) * | 2011-07-06 | 2011-12-21 | 长春迪瑞医疗科技股份有限公司 | Device and method for remaining amount of reagent |
| CN203148516U (en) * | 2013-04-02 | 2013-08-21 | 常州市微凯医疗科技有限公司 | Trace reagent liquid level detecting device for immunohistochemical stainer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108981849A (en) * | 2018-07-26 | 2018-12-11 | 宁波海壹生物科技有限公司 | A kind of fluid level detection system and method for Medical Instruments |
| CN110146141A (en) * | 2019-04-26 | 2019-08-20 | 浙江大学 | Improve the liquid detection device and method of socket type sample needle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103162770B (en) | 2017-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201476844U (en) | Capacitive liquid-level detecting device | |
| CN204963951U (en) | Liquid level detection system | |
| CN204027651U (en) | Test tube sample size pick-up unit | |
| CN201429621Y (en) | Fully automatic sampling instrument | |
| CN203148516U (en) | Trace reagent liquid level detecting device for immunohistochemical stainer | |
| CN104713614B (en) | A kind of liquid level sensor and detection method | |
| CN103162770A (en) | Method and device of trace reagent liquid level detection used for an immunohistochemical staining machine | |
| CN102478417A (en) | Liquid level detection device for sample addition system | |
| CN204373736U (en) | Based on the detecting liquid level circuit of phaselocked loop | |
| CN201983833U (en) | Liquid level detection device of electrolyte analyzer | |
| CN201237520Y (en) | Level detector | |
| CN205209912U (en) | Automatic washing formula detects blood flow graph of blood viscosity | |
| CN205049374U (en) | Two biochemical reagents sample, application of sample devices of selecting response | |
| CN108981849B (en) | A kind of fluid level detection system and method for Medical Instruments | |
| CN209802915U (en) | asphalt dynamic viscosity testing vacuumizing device adopting electronic pressure sensor | |
| CN201697681U (en) | Light control metering device | |
| CN203870085U (en) | Full-automatic alkali activity testing device with rock column method | |
| CN205619988U (en) | Gaseous ration meter of gassiness volume desorption | |
| CN110146141A (en) | Improve the liquid detection device and method of socket type sample needle | |
| CN203758873U (en) | Intelligent engler viscometer | |
| CN202599953U (en) | Blood coagulation detection device | |
| CN204694590U (en) | Multifunctional asphalt pick-up unit | |
| CN201673104U (en) | Full automatic double-station rheogeniometer | |
| CN104034761B (en) | A kind of device and method detecting OBP and pheromone cohesive process | |
| CN108107173A (en) | Portable hand-held water quality detection early-warning apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170222 Termination date: 20180402 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |