CN103160595A - Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene - Google Patents
Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene Download PDFInfo
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- CN103160595A CN103160595A CN201310115903XA CN201310115903A CN103160595A CN 103160595 A CN103160595 A CN 103160595A CN 201310115903X A CN201310115903X A CN 201310115903XA CN 201310115903 A CN201310115903 A CN 201310115903A CN 103160595 A CN103160595 A CN 103160595A
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Abstract
The invention relates to a method for predicting the body height of an excellent ice-snow athlete on the level of cell biology. The method is based on the analysis of the relation between single nucleotide polymorphism (SNP) in a promoter proximal region of a GH1 gene and regulation and control of the body height of a human body, and the relation between the SNP and the body height. The characteristics of the body heights of the existing excellent ice-snow athletes around the world can be investigated by utilizing the method, thereby having a certain reference value for selecting and training talents for the item in China.
Description
Technical field
The present invention relates to a kind of cell biology method of predicting outstanding winter sports person's height.Be suitable for the early stage selection of outstanding winter sports person on gene and protein level, belong to the ambit that sports science and life science are intersected.
Background technology
People's height is a kind of quantitative character of complexity.Winter sports person's final height is by factor interactions such as heredity, environment, psychology, diseases and determine.Studies show that, it is 37%～53% that normal people's height and weight are subjected to the ratio that inherited genetic factors affects.For the quantitative character of this complexity, to remove outside the very low single gene mutation of incidence, the single nucleotide polymorphism (singlenucleotidepolymorphism, SNP) on some associated genes is the hereditary basis that causes mankind's height difference.
Winter sports person's linear growth depends on the stable of growth hormone releasing hormone-growth hormone-insulin-like growth factor-endocrine axis.Wherein most important by the quality and quantity of the tethelin (growth hormone, GH) of adenohypophysis synthesis secretion.After life, the encoding gene of GH is the GH1 gene.The normal people GH1 assignment of genes gene mapping has comprised in 17q22-q24 a gene cluster that is about 66kb that is comprised of 5 genes involveds (GH1, GH2, CSH1, CSH2, CSHP1).The GH1 gene promoter area, especially 5 ' end has shown the polymorphism of height, in the length of GH1 gene promoter proximal end region 535bp, 16 single nucleic acid polymorphisms (SNP) occurred.Studies show that, there is the SNP of dense distribution in the GH1 gene promoter area, and prompting is in the variation of adult's height, has at least 3.3% to be that polymorphism due to the GH1 gene promoter area causes.It is the hereditary basis that causes mankind's height difference that GH1 gene promoter area single nucleotide polymorphism changes.
Generally speaking, GH1 gene genetic characteristics very complex.And its expression product GH plays a very important role for growing of people.Therefore, the GH1 gene polynorphisms should exist more important impact to normal people's height.
Being accompanied by the variation of body shape in athletic developmental process, is basis and an important link in Sports Scientific Research to the research of athletes ' body form especially height.The result of study of Chinese elite winter sports person height feature, to help the foundation of the scientific research system of perfect, system, simultaneously to playing active effect the Chinese elite winter sports person's of structure science, system high model, and establish certain theoretical basis for from now on physical function, technique and tactics, psychology and formation systematic research system.
Since 1998 Nagano Winter Olympic Games short-track speeding skating was formally got permission to become event by demonstration event, 6 pieces of gold medals of this project extremely ice and snow power were attracted attention.Short-track athletic selection and educate also natural receiving publicity of material.Height is as a main aspect of body shape, which characteristic is arranged, whether the height between top performer and non-top performer has difference, which kind of height is more suitable actually according to the characteristics of project, once had before this and specialized in, but As time goes on, the raising of this project level, which new characteristic is athletic height have again, need us further to make more scientific research and deduction, the height feature of inquiring into the outstanding winter sports person in the world today can and be educated the material aspect to the selection of this project of China certain reference value.
The research starting of suitable height is more late to outstanding winter sports person due to China, and winter sports person educate material and the selection major part is all by experience, be not based upon on the basis of life science.In the last few years, along with develop rapidly and both mutual intersection and infiltrations on theory, method, technology and means of sports science and life science, the early stage selection of pusher winter sports person was towards scientific and systematized direction transition and development forcefully.Therefore, be necessary to set up that a cover is perfect, science, the cell biology method of system, effectively predict outstanding winter sports person's the suitableeest height from the cell levels.
Relation in research object between analysis part pleomorphism site genotype and height.Thereby infer the difference condition of specific gene expression amount in cartilaginous tissue, predicted gene is expressed and the contacting of height on the cytobiology level.The polymorphism in GH1 gene promoter area S3:G-308T and S7:T-57G two sites changes the height that all affects winter sports person, causes that winter sports person is of short and small stature.
Summary of the invention
The invention provides a kind of method of predicting outstanding winter sports person's height on the cytobiology level.
The present invention has predicted outstanding winter sports person's the suitableeest height from the cytobiology level, analyze the relation between GH1 part pleomorphism site genotype and height.Method is simple, and is easy to operate.
Embodiment
By a following embodiment, embodiment is described:
Choosing the general population and outstanding winter sports person's peripheral blood distinguishes as a control group and experimental group.
(1) extraction of genomic dna: adopt the benzene phenol-chloroform method to extract genomic dna from peripheral blood ,-20 ℃ save backup.
(2) pcr amplification GH1 gene promoter adjacent domain: adopt touchdown PCR, reaction parameter is: 94 ℃, and 5min; 94 ℃, 30s; 69～59 ℃, 30s (circulate every 10 cycle down low temperature thermal oxidation 1 ℃, rear 15 cycle annealing temperature are 59 ℃), 72 ℃ of 1min circulate 25 times; 72 ℃, 7min.The PCR product is through 1.5% LMA electrophoresis observation result.
(3) PCR product purification, Cloning and sequencing: the purifying of product and clone carry out according to specification sheets in test kit respectively, send order-checking after the clone.
(4) determination and analysis of SNP: finding out two SNP sites, is respectively S3:G-308T and S7:T-57G.
(5) polymorphism in these two sites changes the height that all affects winter sports person, causes that winter sports person is of short and small stature.
Claims (2)
- One kind on the cytobiology level prediction outstanding winter sports person's height method.
- 2. according to claim 1, this method is characterised in that the especially relation between S3:G-308T and S7:T-57G site and height of GH1 part pleomorphism site genotype of analyzing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310115903XA CN103160595A (en) | 2013-04-07 | 2013-04-07 | Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene |
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| CN201310115903XA CN103160595A (en) | 2013-04-07 | 2013-04-07 | Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene |
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| CN103160595A true CN103160595A (en) | 2013-06-19 |
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| CN201310115903XA Pending CN103160595A (en) | 2013-04-07 | 2013-04-07 | Cell biological method for predicting body height of excellent ice-snow athlete by utilizing single nucleotide polymorphism of promoter region of GH1 gene |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349627A (en) * | 2015-10-16 | 2016-02-24 | 柳州市妇幼保健院 | Kit for SNP genotyping of Chinese children short stature susceptibilty genes and utilization method of kit |
-
2013
- 2013-04-07 CN CN201310115903XA patent/CN103160595A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 宋永恒, 沈勋章: "人体身高遗传学研究与运动员基因选材", 《中国优生优育》 * |
| 黄晓萍, 等: "中国部分汉族人群GH1基因启动子区单核苷酸多态性与正常人群身高的关系", 《上海交通大学学报（医学版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349627A (en) * | 2015-10-16 | 2016-02-24 | 柳州市妇幼保健院 | Kit for SNP genotyping of Chinese children short stature susceptibilty genes and utilization method of kit |
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Application publication date: 20130619 |