CN103159977A - Method for preparing polylactic acid porous support material - Google Patents
Method for preparing polylactic acid porous support material Download PDFInfo
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- CN103159977A CN103159977A CN 201110414695 CN201110414695A CN103159977A CN 103159977 A CN103159977 A CN 103159977A CN 201110414695 CN201110414695 CN 201110414695 CN 201110414695 A CN201110414695 A CN 201110414695A CN 103159977 A CN103159977 A CN 103159977A
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- lactic acid
- polylactic acid
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Abstract
The present invention discloses a method for preparing polylactic acid porous support material. By changing the conditions in the preparation process, such as ratio of dioxane and water, concentration of a polylactic acid solution, coarsening temperature, coarsening time, and the number of the coarsenings, the micromorphological structure of a porous support such as pore size, pore morphology and porosity can be effectively controlled. The pore size of 5-400 microns is controllable, breaking material pore size ceiling of 200 microns by the conventional thermally induced phase separation. The method has a very important significance for the preparation of porous tissue engineering materials.
Description
Technical field
The invention belongs to the organizational engineering technical field, be specifically related to a kind of polylactic acid porous scaffold for tissue engineering material preparation method.
Background technology
Organizational project is 21st century life science one large focus.Its core content is the cell in vitro dimensional culture, and its basic skills is that seed cell and degradable biomaterial are formed complex body, substitutes the damaged tissue organ.The porous polymer tissue engineering material is due to analog cell epimatrix well, and is widely used in the middle of the organizational projects such as bone, cartilage, skin, liver.Porous support as used in tissue engineering need to have suitable mechanical property to carry certain load, good biocompatible surface is arranged to help cell successfully to adhere to growth, have the structure that is interconnected of suitable aperture and porosity and height to transport so that cell enters support and nutritive substance and products of cellular metabolism.Different histocytes has different requirements to the aperture, needs the aperture of 20 μ m left and right as inoblast, skin histology rebuild need support the aperture between 20 μ m to 150 μ m, bone and the cartilage tissue engineered aperture that needs 100-250 μ m.Thermally induced phase separation is a kind of important method of preparation porous polymer material.The method is simple, and inclusion-free is residual, and the gained porosity of porous material highly is communicated with between high and hole, and is widely used in the preparation of poromerics.But common thermally induced phase separation is difficult to the very large porous material in preparation aperture, thereby has limited the application of thermally induced phase separation in organizational project.
Summary of the invention
The object of the invention is to overcome present thermally induced phase separation and prepare porous support aperture problem less than normal, the preparation method of the wider Novel porous polylactic acid timbering material of a kind of pore diameter range is provided.
The technical solution adopted in the present invention is:
A kind of polylactic acid porous scaffold material preparation method is characterized in that comprising the following steps:
1) poly(lactic acid) is dissolved in the mixed solvent of dioxane and water at 40 ℃ of 80 ℃ of temperature, gets poly(lactic acid) solution, the bulking value concentration of poly(lactic acid) solution is 1 10%, and the volume ratio of each mixed solvent dioxane and water is 10,0/1 80/20;
2) above-mentioned poly(lactic acid) solution is injected mould, alligatoring 1 12 hours, Coarsening Temperature is 10 ℃ 50 ℃, after alligatoring, under-10 ℃-100 ℃ freezing 0.5 3 hours, then freeze thousand in Freeze Drying Equipment, get polylactic acid porous scaffold of the present invention.
The said alligatoring of the present invention refers to below phase separation temperature, and certain above temperature of solvent temperature of solidification stays for some time.Alligatoring can only be carried out once, also can carry out continuously several.
Described poly(lactic acid) is one or more in poly-L-lactic acid, poly-0 lactic acid or poly-DL lactic acid.
The material of described mould is stainless steel or glass or the plastics of anti-the solvent.
The beneficial effect that technical solution of the present invention is brought is:
The present invention passes through to change the various conditions in preparation process, as the ratio of dioxane and water, and the poly(lactic acid) strength of solution, Coarsening Temperature, coarsening time, alligatoring number of times, can effectively control microscopic pattern structure such as aperture, pore morphology and the porosity of the porous support of gained.Make the aperture controlled between 5 400 microns, broken through traditional thermally induced phase separation and prepared 200 microns of the material aperture upper limits, have very important significance for the multi-porous tissue engineering material preparation.It is high that the porous material that the present invention makes possesses the porosity that thermally induced phase separation had originally simultaneously, simple process, the advantage such as inclusion-free is residual.The porous support for preparing by the present invention can be widely used in reconstruction and the regeneration of the Various Tissues such as skin, cartilage, bone, blood vessel, tracheae, oesophagus, tendon, heart valve or organ.
Embodiment:
The invention is further illustrated by the following examples, but the present invention is not limited to this.
Embodiment 1
The polylactic acid porous scaffold material preparation
Poly(lactic acid) is dissolved in the mixed solvent of dioxane and water at 40 ℃ of 80 ℃ of temperature in, get poly(lactic acid) solution, the bulking value concentration of poly(lactic acid) solution is 1 10%, and the volume ratio of each mixed solvent dioxane and water is 10,0/1 80/20; Above-mentioned poly(lactic acid) solution is injected mould, alligatoring 1 12 hours, Coarsening Temperature is 10 ℃ 50 ℃, after alligatoring, under-10 ℃-100 ℃ freezing 0.5 3 hours, then freeze thousand in Freeze Drying Equipment, get polylactic acid porous scaffold of the present invention.
Embodiment 2
Poly(lactic acid) liquid concentration is to the porous support performance impact
Poly-L-lactic acid is dissolved in dioxane under 80 ℃: in the mixed solvent of water (volume ratio 85/15), the bulking value concentration of poly(lactic acid) be respectively 4%, 5% and 6%(w/v), be poured in diameter 1.2cm thickness 8cm glass mold, under 25 ℃, after alligatoring 5 hours, under-20 ℃ freezing 1 hour, then freeze-drying can obtain the poly-L-lactic acid three-dimensional porous rack in 24 hours in Freeze Drying Equipment.Poly(lactic acid) liquid concentration sees Table 1 to the impact of porous support aperture and porosity.
The impact of table 1 poly(lactic acid) liquid concentration on porous support aperture and porosity
Claims (3)
1. polylactic acid porous scaffold material preparation method is characterized in that comprising the following steps:
1) poly(lactic acid) is dissolved in the mixed solvent of dioxane and water at 40 ℃ of 80 ℃ of temperature, gets poly(lactic acid) solution, the bulking value concentration of poly(lactic acid) solution is 1 10%, and the volume ratio of each mixed solvent dioxane and water is 10,0/1 80/20;
2) above-mentioned poly(lactic acid) solution is injected mould, alligatoring 1 12 hours, Coarsening Temperature is 10 ℃ 50 ℃, after alligatoring, under-10 ℃-100 ℃ freezing 0.5 3 hours, then freeze thousand in Freeze Drying Equipment, get polylactic acid porous scaffold of the present invention.
2. polylactic acid porous scaffold material preparation method according to claim 1 is characterized in that: described poly(lactic acid) is one or more in poly-L-lactic acid, poly-O lactic acid or poly-DL lactic acid.
3. polylactic acid porous scaffold material preparation method described according to claims 1, it is characterized in that: the material of described mould is stainless steel or glass or the plastics of anti-the solvent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110414695 CN103159977A (en) | 2011-12-13 | 2011-12-13 | Method for preparing polylactic acid porous support material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110414695 CN103159977A (en) | 2011-12-13 | 2011-12-13 | Method for preparing polylactic acid porous support material |
Publications (1)
| Publication Number | Publication Date |
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| CN103159977A true CN103159977A (en) | 2013-06-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110414695 Withdrawn CN103159977A (en) | 2011-12-13 | 2011-12-13 | Method for preparing polylactic acid porous support material |
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| CN (1) | CN103159977A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432782A (en) * | 2016-08-01 | 2017-02-22 | 郑州大学 | Hydrophobic porous polymer integral material with nest-like structure and preparation method thereof |
| CN109825047A (en) * | 2019-01-31 | 2019-05-31 | 南通大学 | A kind of polylactic acid porous material of superabsorbent water and preparation method thereof |
| CN110694105A (en) * | 2019-09-17 | 2020-01-17 | 东南大学 | Degradable metal wire directionally-reinforced polylactic acid porous bone repair material and preparation method thereof |
| CN110947035A (en) * | 2019-10-30 | 2020-04-03 | 中山大学附属第六医院 | Method for modifying surface of P4HB patch in porous manner and P4HB patch |
-
2011
- 2011-12-13 CN CN 201110414695 patent/CN103159977A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432782A (en) * | 2016-08-01 | 2017-02-22 | 郑州大学 | Hydrophobic porous polymer integral material with nest-like structure and preparation method thereof |
| CN109825047A (en) * | 2019-01-31 | 2019-05-31 | 南通大学 | A kind of polylactic acid porous material of superabsorbent water and preparation method thereof |
| CN109825047B (en) * | 2019-01-31 | 2022-07-05 | 北京朗净汇明生物科技有限公司 | Super water-absorbent polylactic acid porous material and preparation method thereof |
| CN110694105A (en) * | 2019-09-17 | 2020-01-17 | 东南大学 | Degradable metal wire directionally-reinforced polylactic acid porous bone repair material and preparation method thereof |
| CN110947035A (en) * | 2019-10-30 | 2020-04-03 | 中山大学附属第六医院 | Method for modifying surface of P4HB patch in porous manner and P4HB patch |
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Application publication date: 20130619 |