CN103142734B - A kind of Chinese medicine compound composition and activity rating thereof with broad-spectrum antibacterial action - Google Patents
A kind of Chinese medicine compound composition and activity rating thereof with broad-spectrum antibacterial action Download PDFInfo
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- CN103142734B CN103142734B CN201110430385.1A CN201110430385A CN103142734B CN 103142734 B CN103142734 B CN 103142734B CN 201110430385 A CN201110430385 A CN 201110430385A CN 103142734 B CN103142734 B CN 103142734B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The present invention relates to the prescriptions of Chinese medicine composition and activity rating with broad spectrum antibiotic activity, belong to medical art.Supercritical extraction and ethanol reflux extraction is utilized to obtain crude extract prescription from Hypericum ascyron, Cortex cinnamomi japonici (Ramulus Cinnamomi), Fructus Citri grandis.It is characterized in that easy and simple to handle, the time is short, without precipitation, and storage life limit for length, not easily spoiled.The prescriptions of Chinese medicine that the present invention obtains has broad spectrum antibiotic activity, to 27 kinds of common bacterias, there is good antibacterial action, comprise the fastbacteria such as methicillin-resistant staphylococcus aureus and resistance to quinolinones staphylococcus aureus, can be used as the raw material of natural antibacterial agent and antiseptic.
Description
Affiliated field:
The present invention relates to a kind of Chinese medicine compound, the especially a kind of preparation of natural plant compound spray and activity rating thereof with broad-spectrum antibacterial action.
Background technology:
On in the whole society of western medicine antibiotics " limit is sold ", Chinese medicine enterprise of China benefits from it, Chinese medicine manufacturing enterprise to surge several times at output, sales volume, the concentration degree of traditional Chinese medicine industry gets a promotion, and the Chinese medicine word such as people's more unfamiliar positive source pellet, JIZHI TANGJIANG, Bufo siccus pin, TONGLUO SHENGGU JIAONANG has incorporated the health care life of people quickly at ordinary times.But after " limit sells hurricane ", bringing business opportunity both to Chinese medicine enterprise, will be to the once test of China's Chinese medicine in product innovation capability again.Nearly 2 years, China's Chinese Traditional Medicine research and production were marched toward the recovery stage comprehensively.SARS, China's Chinese medicine has showed advantage at antibiosis, and some common anti-microbial type Chinese patent medicines instead of antibiotic medicine, and Chinese medicine antibiotic is given full play to one's remarkable skill on Western medicine medicine altar.And in this year, along with China's " Chinese medicine antibiotic " researches and develops the rise of tide, Chinese medicine antibiotic pharmacy mechanism achieves large progress.In the infection mechanism of medicine, antibacterial action is a very important content.
The plant amedica be made up of plant extract position is in recent years gradually for various countries accept, even if FDA Food and Drug Administration also starts to pay attention to plant amedica development, and promulgated " plant medicines production guide " draft, consider to agree to that accepting the new drug that is made up of plant extract position carries out clinical research, but the quality of this extract part and the stability of drug effect and concordance must be ensured.If the active substance that can make full use of in plant makes antibiotic, be a kind of effective behave of the useful human health be worthy to be popularized really.Modern consumption theory by health drives, and the consumption market of China has presented to the more and more vigorous Requirement of antibacterial the articles for daily use.The research and development of China's anti-biotic material and articles for use are started late, and existing antibacterial product kind is also fewer, and many products are in the market introduction stage, far can not meet the needs in market.There is the tradition using Chinese medicine in China, and the Chinese herbal medicine practice of 1,100 has confirmed that numerous Chinese herbal medicine all has effect of antiphlogistic antibacterial.Find hypotoxicity, the natural product with broad-spectrum antibacterial action becomes the object of study of numerous scholar.
At present, open or commercial liquid spray agent prescription, mostly is chemicals or chemicals and herb liquid mixture wherein for antibiotic skin-care preparation, does not also find the pure Chinese medicinal preparation with broad spectrum antibiotic activity.
Summary of the invention:
The present invention is the mixture substituting chemicals or chemical drugs and Chinese medicine with pure Chinese medicine, provide can change antibiotic medicine and bring drug resistance, side effect, reach again that production method is simple, health, Chinese medicine compound with broad-spectrum antibacterial action and preparation method thereof.The object of the invention is to be achieved through the following technical solutions:
1. compound Chinese medicinal preparation preparation technology is as follows:
(1) Hypericum ascyron extraction process is,
Supercritical extraction process: the Hypericum ascyron getting drying and crushing loads extraction kettle, and be 25MPa in extracting pressure, temperature is 45 DEG C, CO
2flow is extract 2h under the condition of 143.5kg/L, obtains Hypericum ascyron extract.
(2) get dry Fructus Citri grandis, add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter extracting solution after 2 hours, in medical material, adding the ethanol of isodose, reflux, extract, was filtered after 1 hour, merged extracted twice liquid, was concentrated into extractum.
(3) get Cortex cinnamomi japonici (Ramulus Cinnamomi), add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter extracting solution after 2 hours, in medical material, adding the ethanol of isodose, reflux, extract, filtered extracting solution after 1 hour, merged extracted twice liquid, was concentrated into extractum.
(4) compound recipe consists of: Hypericum ascyron extract 1g, grapefruit abstract 2g, Cortex cinnamomi japonici (Ramulus Cinnamomi) extract 4g, add dehydrated alcohol 100mL, distilled water 100mL, for subsequent use after dissolving completely
2. antibacterial activity evaluation:
Extracorporeal bacteria inhibitor test: adopt the double dilution method of 96 well culture plate, with MuellerHintonBroth and BrainHeartInfusion for culture medium, antibacterial experiment in vitro research has been carried out to Hypericum ascyron extract, grapefruit abstract, Cortex cinnamomi japonici (Ramulus Cinnamomi) extract and compound recipe, result shows: (1) shows for the test result of gram positive bacteria, and Hypericum ascyron compound recipe all has good antibacterial activity for most gram positive bacteria.(2) show the test result of gram-negative bacteria, Hypericum ascyron compound recipe is stronger than the antibacterial activity of vancomycin.(3) to methicillin-resistant staphylococcus aureus and resistance to quinolinones staphylococcus aureus, there is obvious activity, and minimal inhibitory concentration is 2 ~ 4 μ g/mL, has good DEVELOPMENT PROSPECT.(4) good antibacterial action is also had to Candida albicans.
Have report to prove edible at Hypericum ascyron, in compound recipe, Fructus Citri grandis is the fruit often used, and Cortex cinnamomi japonici (Ramulus Cinnamomi) is flavouring agent is all spendable, and it does not almost have Side effect, proves that compound recipe has good Development volue and market application foreground.
3. prescription composition: the compound preparation that the present invention obtains belongs to pure natural antibacterial goods, has special aroma.
Detailed description of the invention:
1. Chinese medicine compound preparation technology is as follows
(1) Hypericum ascyron extraction process:
Supercritical extraction process: the Hypericum ascyron 1.2kg getting drying and crushing loads extraction kettle, and be 25MPa in extracting pressure, temperature is 45 DEG C, CO
2flow is extract 2h under the condition of 143.5kg/L, and obtain Hypericum ascyron extract 12g, yield is 1%.
(2) get dry Fructus Citri grandis 100g, add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter after 2 hours, medicinal residues add the ethanol of isodose, and reflux, extract, was filtered after 1 hour, merge extracted twice liquid, and concentrate to obtain extractum 26.2g, yield is 26.2%.
(3) get Cortex cinnamomi japonici (Ramulus Cinnamomi) 100g, add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter after 2 hours, medical material adds the ethanol of isodose, and reflux, extract, was filtered after 1 hour, and merge extracted twice liquid and concentrate to obtain extractum 26g, yield is 26%.
(4) compound recipe consists of: Hypericum ascyron extract 1g, grapefruit abstract 2g, Cortex cinnamomi japonici (Ramulus Cinnamomi) extract 4g, add dehydrated alcohol 100mL, distilled water 100mL, for subsequent use after dissolving completely
2. antibacterial activity evaluation:
Extracorporeal bacteria inhibitor test: adopt the double dilution method of 96 well culture plate, with MuellerHintonBroth and BrainHeartInfusion for culture medium, according to different condition of culture to staphylococcus aureus, methicillin-resistant staphylococcus aureus, resistance to quinolinones staphylococcus aureus, bacillus subtilis, Bacillus cercus, Streptococcus mutans, enterococcus faecalis, escherichia coli, typhoid fever sramana (family name) bacillus, produce sour Cray uncle formula bacterium, bacillus pyocyaneus, citrobacter freundii, Bacillus proteus, serratia marcesens, clostridium perfringen, Acinetobacter calcoaceticus, the antibacterials such as Candida albicans and fungus have carried out antibacterial experiment in vitro research, by showing that to the antibacterial activity result of single medical material and its compound recipe the antibacterial activity of compound preparation is all higher than the antibacterial activity of single medical material, and there is good broad-spectrum antimicrobial effect.
The antibacterial activity evaluation result of table single medicinal material and compound recipe
Claims (2)
1. a compound Chinese medicinal preparation, the preparation method of this compound Chinese medicinal preparation comprises the steps:
(1) Hypericum ascyron extraction process:
Supercritical extraction process: the Hypericum ascyron 1.2kg getting drying and crushing loads extraction kettle, and be 25MPa in extracting pressure, temperature is 45 DEG C, CO
2flow is extract 2h under the condition of 143.5kg/L, and obtain Hypericum ascyron extract 12g, yield is 1%;
(2) get dry Fructus Citri grandis 100g, add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter after 2 hours, medicinal residues add the ethanol of isodose, and reflux, extract, was filtered after 1 hour, merge extracted twice liquid, and concentrate to obtain extractum 26.2g, yield is 26.2%;
(3) get Cortex cinnamomi japonici (Ramulus Cinnamomi) 100g, add 95% appropriate ethanol reflux, extract, at 80 DEG C and filter after 2 hours, medical material adds the ethanol of isodose, and reflux, extract, was filtered after 1 hour, and merge extracted twice liquid and concentrate to obtain extractum 26g, yield is 26%;
(4) compound Chinese medicinal preparation composition: Hypericum ascyron extract 1g, grapefruit abstract 2g, Cortex cinnamomi japonici (Ramulus Cinnamomi) extract 4g, add dehydrated alcohol 100mL, distilled water 100mL, for subsequent use after dissolving completely;
Described compound Chinese medicinal preparation to staphylococcus aureus, methicillin-resistant staphylococcus aureus, resistance to quinolinones staphylococcus aureus, bacillus subtilis, Bacillus cercus, Streptococcus mutans, enterococcus faecalis, escherichia coli, typhoid fever sramana (family name) bacillus, produce sour Cray uncle formula bacterium, bacillus pyocyaneus, citrobacter freundii, Bacillus proteus, serratia marcesens, clostridium perfringen, Acinetobacter calcoaceticus, Candida albicans have antibacterial activity.
2. compound Chinese medicinal preparation according to claim 1, is characterized in that, described compound Chinese medicinal preparation makes any one preparation of capsule, oral liquid, distillate medicinal water, electuary, suppository, ointment, spray.
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| CN115746963A (en) * | 2023-01-06 | 2023-03-07 | 中央民族大学 | Preparation method of hypericum active volatile oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2236149A1 (en) * | 2008-01-29 | 2010-10-06 | Nakamori Pharmaceutical Co., Ltd. | Medicinal composition |
| CN101874844A (en) * | 2010-04-16 | 2010-11-03 | 范怀蒙 | Citrus extract bacteriostat, production process and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2236149A1 (en) * | 2008-01-29 | 2010-10-06 | Nakamori Pharmaceutical Co., Ltd. | Medicinal composition |
| CN101874844A (en) * | 2010-04-16 | 2010-11-03 | 范怀蒙 | Citrus extract bacteriostat, production process and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 桂皮提取物成分分析及抗菌活性的研究;张慧芸等，;《食品研究与开发》;20100331;第31卷(第3期);第147-150页 * |
| 沙田柚油皮提取物的抗菌性能研究;蒋玲艳等，;《安徽农业科学》;20081231;第36卷(第22期);第9354-9355, 9357页 * |
| 贯叶金丝桃活性成分及其分离纯化与检测方法的研究进展;李艳等，;《药学进展》;20081231;第32卷(第1期);第15-20页 * |
| 长柱金丝桃的研究进展;刘利军等，;《绥化学院学报》;20060630;第26卷(第3期);第155-156页 * |
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