CN103134801B - Microscopic identification method for ginseng spleen-strengthening pills - Google Patents
Microscopic identification method for ginseng spleen-strengthening pills Download PDFInfo
- Publication number
- CN103134801B CN103134801B CN201310072495.4A CN201310072495A CN103134801B CN 103134801 B CN103134801 B CN 103134801B CN 201310072495 A CN201310072495 A CN 201310072495A CN 103134801 B CN103134801 B CN 103134801B
- Authority
- CN
- China
- Prior art keywords
- cell
- reference substance
- micro
- ginseng
- strengthening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a microscopic identification method for ginseng spleen-strengthening pills. The method comprises the specific steps of: (1) constructing a ginseng spleen-strengthening pill raw medicine and traditional Chinese medicine contrast micrographia and a cell tissue database thereof; (2) shooting, observing and measuring cell tissue characteristics of a microscopic film on the micrographia by the same method in the step (1); and (3) comparing and analyzing the various characteristics such as the colors, the shapes and the textures of the micrographia and a cell tissue of a tested micrographia and a contrast, and obtaining an identification result. The method has the advantages that (1) the microscopic characteristic description of the ginseng spleen-strengthening pill raw medicine and traditional Chinese medicine contrast micrographia database is higher in specificity than that of the Chinese pharmacopoeia, so that the quality detection accuracy is greatly improved; and (2) the falseness of ginseng spleen-strengthening pill raw medicines can be accurately and qualitatively analyzed, so that mixtures of medicines during production of semi-finished and finished traditional Chinese medicine products and a phenomenon of incomplete cleaning are avoided.
Description
Technical field
The present invention relates to a kind of method of ginseng spleen-strengthening bolus Microscopic Identification.
Background technology
Current Chinese traditional medicine identification mainly comprises the 4 large classes such as base chiller, Characters Identification, Microscopic Identification and physics and chemistry qualification, and Microscopic Identification is one of main method of Chinese medical herb now.TCD MICROSCOPIC IDENTIFICATION utilizes microtechnic to carry out microscopic analysis to Chinese medicine, to determine a kind of authentication method of its kind and quality.Microscopic Identification mainly comprises tissue characterization and powder qualification, utilizes microscope to observe the histological structure of medicinal material, the character of cell shape and inclusions or some kind effective ingredient distribution etc. in the tissue, in order to differentiate the true and false and the purity even quality of medicinal material.For the medicinal material by the close multi-source medicinal material of proterties medicinal material not easy to identify, proterties or chopping and pulverulence, and with the Chinese patent drug that powder is made, Microscopic Identification is even more important.Various in style due to China's Chinese medicine, the phenomenon of Chinese medicine true and false confusion happens occasionally, and brings great difficulty to qualification work.
" Chinese Pharmacopoeia " version regulation Chinese medicine preparation in 2010 feeds intake and uses the prepared slices of Chinese crude drugs without exception, and regulation has been done to crude drug source, medicinal part, because Preparation process is lack of standardization, a lot of dis-medicinal part is easily mixed into wherein, uses Microscopic Identification technology effectively can ensure the authenticity fed intake.The 11 taste Chinese crude drug microscopic features totally 40 related in ginseng spleen-strengthening bolus in " Chinese Pharmacopoeia ", specificity is strong not.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of method of ginseng spleen-strengthening bolus Microscopic Identification.
The present invention adopts following technical scheme:
Concrete steps of the present invention are as follows:
One, ginseng spleen-strengthening bolus raw medicinal material and the micro-collection of illustrative plates of Chinese patent drug reference substance and cell tissue database thereof is set up:
Microscopic features 64 are had in the micro-collection of illustrative plates of described ginseng spleen-strengthening bolus raw medicinal material reference substance and cell tissue database thereof, comprising the microscopic features in " Chinese Pharmacopoeia " 40, the microscopic features newly increased 24;
Total microscopic features 11 in the database of the micro-collection of illustrative plates of described ginseng spleen-strengthening bolus Chinese patent drug reference substance and cell tissue thereof, comprising the microscopic features in " Chinese Pharmacopoeia " 3, the microscopic features newly increased 8;
(1) ginseng spleen-strengthening bolus raw medicinal material and the film-making of ginseng spleen-strengthening bolus Chinese patent drug reference substance:
(1) powder film-making: reference substance powder is crossed No. five sieves, picking is put on microslide a little, drips glycerine acetic acid test solution or chloral hydrate test solution, covered; If desired, heat penetration is added;
(2) ginseng spleen-strengthening bolus Chinese patent drug reference substance microsection: get the ginseng spleen-strengthening bolus water-bindered pill, water-honeyed pill, large honeyed bolus and each several ball of small honey pill, put pulverize in mortar respectively, get appropriate powder; Pill should cut by honeyed bolus, from tangent plane by central picking appropriate amount of sample or after taking off honey with water, draw sediment a small amount of; By the film-making of above-mentioned powder method of tableting;
(2) micrometering is carried out to the micro-collection of illustrative plates of described reference substance:
Micrometering refers to the size measuring cell and ergastic mateiral under the microscope with eyepiece micrometer; First by eyepiece micrometer microscope carrier micrometer footage number, calculate μm number of each little lattice, will the little lattice number of object be recorded during application, and be multiplied by μm number of each little lattice, obtain the size of predicted earnest;
(3) the micro-collection of illustrative plates of described reference substance is observed and imaging:
" microscope image pick-up with the automated imaging of display and memory storage " is adopted to carry out microexamination;
First microsection is placed on microscopical objective table during microexamination, micro-image candid photograph is carried out to the cell observed, tissue structure shape and content shape, and stored in corresponding database; Next measures the size of cell, tissue, is placed in by microsection on microscopical objective table, by eyepiece micrometer and objective table micrometer, measures maximal value and the minimum value of cell, tissue structure shape and content geomery; During observation, first using 10 times of object lens to observe with Nosepiece, when observing object, rotating Nosepiece, use 40 times of object lens to aim at it and measure observation, then take, Image Saving in specified database; During microscopy, described reference substance microslide first should move along X-axis and observe, then moves along Y-axis and observe, in case omit;
Two, by the method identical with above-mentioned steps one, the candid photograph of micro-collection of illustrative plates, observation, measurement cell tissue feature are carried out to test specimen microsection;
Three, the micro-collection of illustrative plates of micro-for test specimen collection of illustrative plates and reference substance and cell tissue color, form, each feature of texture are carried out contrast and analysis, draw qualification result.
In the present invention, the collection process of described microscopic features is as follows:
(1) micro-collection of illustrative plates is collected;
(2) micrograph spectrum information is read;
(3) microscopic features are extracted;
Described microscopic features comprise color characteristic, contour feature, textural characteristics and length characteristic;
(4) by above-mentioned microscopic features stored in described database.
In the present invention, the micro-collection of illustrative plates of test specimen and reference substance micro-collection of illustrative plates comparative analysis process as follows:
One, test specimen proper vector is obtained:
(1) micro-for test specimen collection of illustrative plates is extracted the region that will contrast through Image semantic classification;
(2) feature extraction is carried out;
(3) test specimen proper vector is obtained;
Two, reference substance proper vector stored in reference substance feature database is obtained:
(1) the micro-collection of illustrative plates in reference substance micrograph modal data storehouse is carried out Image semantic classification and extract the region that will contrast;
(2) feature extraction is carried out;
(3) reference substance proper vector stored in reference substance feature database is obtained;
Three, test specimen proper vector is carried out characteristic matching to the corresponding reference substance proper vector in reference substance feature database.
In the present invention, 24 microscopic features newly increasing of described ginseng spleen-strengthening bolus raw medicinal material are as follows:
(1) bighead atractylodes rhizome increases by 2, i.e. cork cell and test-tube baby;
(2) Radix Angelicae Sinensis increases by 2, i.e. cork cell and amylum body;
(3) Radix Astragali increases by 3, i.e. amylum body, cork cell and prothenchyma (of wood);
(4) banksia rose increases by 2, i.e. cork cell and parenchyma cell;
(5) ginseng increases by 1, i.e. wood-parenchymatous cell;
(6) fructus amomi increases by 3, i.e. cluster crystal, aril cell and pigment block;
(7) Chinese yam increases by 2, i.e. fiber and screen casing;
(8) wild jujube increases by 3, i.e. brown parenchyma cell, endosperm and cotyledon cell and conduit;
(9) polygala root increases by 6, namely calcium oxalate cluster crystal, cork cell, parenchyma cell, xylogen single be dispersed in or bunchy, conduit, wood-parenchymatous cell.
In the present invention, 8 microscopic features newly increasing of described ginseng spleen-strengthening bolus Chinese patent drug are as follows:
(1) for the bighead atractylodes rhizome in ginseng spleen-strengthening bolus: needle-like calcium oxalate crystal is tiny, long 10 ~ 32 μm, be filled in brokenly in parenchyma cell;
(2) for the Radix Angelicae Sinensis in ginseng spleen-strengthening bolus: parenchyma cell spindle, wall is slightly thick, has atomic thin cross lamination;
(3) for the Poria cocos in ginseng spleen-strengthening bolus: irregular branch agglomerate is colourless, meet chloral hydrate liquid and dissolve; Hyphae colorless or light brown;
(4) for the dried orange peel in ginseng spleen-strengthening bolus: calcium oxalate prismatic crystal is present in parenchyma cell in flakes;
(5) for the banksia rose in ginseng spleen-strengthening bolus: xylogen bunchy, spindle shape, diameter 16 ~ 24 μm, wall is slightly thick, pit aperture transverse fissure gap-like, crosswise or people's shape; Reticulate vessel diameter about to 90 μm;
(6) for the astragalus root in ginseng spleen-strengthening bolus: fiber bunchy or fall apart from, wall thickness, there is longitudinal crack on surface, and two ends fragment into broom shape or more truncate;
(7) for the polygala root in ginseng spleen-strengthening bolus: cork cell sees in polygon on surface. the square or rectangle like of class, anticline is thinner, has pit, in interrupted shape;
(8) for the wild jujube in ginseng spleen-strengthening bolus: endotesta cell brown color, surface see rectangle or class square, anticline beaded thickens.
Beneficial effect of the present invention:
1, the microscopic features in ginseng spleen-strengthening bolus raw medicinal material and Chinese patent drug micrograph modal data storehouse describe and have stronger specificity than the microscopic features description in " Chinese Pharmacopoeia ", greatly can improve the accuracy rate of the quality testing of ginseng spleen-strengthening bolus raw medicinal material and Chinese patent drug.
2, the foundation in micrograph modal data storehouse and microscopic features present more directly perceived, concrete, vivid than " Chinese Pharmacopoeia " text description, what database microscopic features described simultaneously increases, increase item feature and can play booster action to micro-qualitative detection, add microexamination possibility qualitatively as early as possible, more convenient tester contrasts, and makes quality detection work efficient, quick.
3, while accurate qualitative analysis being carried out to the true and false of ginseng spleen-strengthening bolus raw medicinal material, effectively can stop Chinese patent drug semi-manufacture, finished product occurs the adulterant of medicinal material and halfway phenomenon of clearing out a gathering place in producing.
4, have that the calibration interval are short, testing cost is low, the feature of environmental protection.
Accompanying drawing explanation
Fig. 1 is microscopic features collection process figure;
Fig. 2 is the micro-collection of illustrative plates of test specimen and reference substance micro-collection of illustrative plates comparative analysis procedure chart;
Fig. 3 is the microscopic features figure of the bighead atractylodes rhizome;
Fig. 4 is the microscopic features figure of dried orange peel;
Fig. 5 is the microscopic features figure of Radix Angelicae Sinensis;
Fig. 6 is the microscopic features figure of Poria cocos;
Fig. 7 is the microscopic features figure of the Radix Astragali;
Fig. 8 is the microscopic features figure of the banksia rose;
Fig. 9 is the microscopic features figure of ginseng;
Figure 10 is the microscopic features figure of fructus amomi;
Figure 11 is the microscopic features figure of Chinese yam;
Figure 12 is the microscopic features figure of wild jujube;
Figure 13 is the microscopic features figure of polygala root;
Figure 14 is the microscopic features figure of Chinese yam in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 15 is the microscopic features figure of ginseng in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 16 is the microscopic features figure of fructus amomi in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 17 is the microscopic features figure of the bighead atractylodes rhizome in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 18 is the microscopic features figure of Radix Angelicae Sinensis in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 19 is the microscopic features figure of Poria cocos in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 20 is the microscopic features figure of dried orange peel in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 21 is the microscopic features figure of the banksia rose in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 22 is the microscopic features figure of the Radix Astragali in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 23 is the microscopic features figure of polygala root in ginseng spleen-strengthening bolus Chinese patent drug reference substance;
Figure 24 is the microscopic features figure of wild jujube in ginseng spleen-strengthening bolus Chinese patent drug reference substance.
Embodiment
Embodiment:
According to the technical scheme concrete operations in foregoing invention content part.
The microscopic features figure of ginseng spleen-strengthening bolus raw medicinal material reference substance is provided below, uses in qualification:
One, get Different sources, the different year bighead atractylodes rhizome three parts, carry out microscopic observation, observe microscopic features as shown in Figure 3 simultaneously:
The microscopic features that the bighead atractylodes rhizome describes in " Chinese Pharmacopoeia " have following 5 (see figure 3)s:
1. needle-like calcium oxalate crystal is tiny, long 10 ~ 32 μm, is present in parenchyma cell, minority needle diameter to 4 μm.
2. fiber yellow, most bunchy, spindle shape, diameter about to 40 μm, wall is very thick, lignify, and hole ditch is obvious.
3. lithocyte is faint yellow, similar round, polygon, rectangle or minority spindle, diameter 37 ~ 64 μm.
4. parenchyma cell is containing synanthrin, the aobvious radial texture in surface.
5. vessel element is short and small, is reticulate vessel and bordered pit vessel, diameter to 48 μm.
By repeatedly microexamination, in addition to those described above, possess following characteristics 2 (see figure 3)s simultaneously:
6. cork cell surface is seen in class polygon, and wall is quite thin, micro-lignify, the faint yellow content of some conjunctions.Some link cork stone cells.
7. test-tube baby is bordered pit and reticulate trancheid, diameter about to 32 μm.
Two, get Different sources, different year dried orange peel three parts, carry out microscopic observation, observe microscopic features as shown in Figure 4 simultaneously:
Dried orange peel describes microscopic features in " Chinese Pharmacopoeia " following 5 (see figure 4)s:
1. mesocarp parenchymal tissue is numerous, and cell shape is irregular, and wall is uneven to be thickened, some one-tenth beadeds.
2. pericarp epidermal surface sees the square or rectangle of polygon, class, and anticline is slightly thick, pore similar round, diameter 18 ~ 26 μm, and accessory cell is unintelligible; Side is seen outer by cuticula, and the radial wall of outer side thickens.
3. calcium oxalate prismatic crystal is present in mesocarp parenchyma cell in flakes, in polyhedron shape, rhombus or biconial, and diameter 3 ~ 34 μm, long 5 ~ 53 μm, containing the parallel twin be made up of two polyhedrons or 3 ~ 5 prismatic crystals in the cell had.
4. hesperidin crystal is present in parenchyma cell mostly, yellow or colourless, rounded or amorphous agglomerate, the visible radial-like streak had.
5. pitted vessel, spiral duct and reticulate vessel and test-tube baby less.
Three, get Different sources, different year Radix Angelicae Sinensis three parts, carry out microscopic observation, observe microscopic features as shown in Figure 5 simultaneously:
Radix Angelicae Sinensis describes microscopic features at " Chinese Pharmacopoeia " following 3 (see figure 5)s:
1. phloem parenchyma cell spindle, wall is slightly thick, and there is atomic oblique cross lamination carefully on surface, sometimes visible poor tabula.
2. scalariform duct and reticulate vessel common, diameter about to 80 μm.
3. visible grease chamber fragment sometimes.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 2 (see figure 5)s:
4. cork cell is faint yellow.Surface is seen in class polygon, size not-, wall is thin, and it is flat that section sees cell.
5. spherical, the kidney shape of amylum body simple grain class or polygon, diameter 3 ~ 22 μm, omphalion point-like, people's shape or three forked, how composite grain minority, be made up of 2 ~ 4 gradation.In addition, even visible xylogen, diameter 26 ~ 31 μm, wall thickness, micro-lignify, pit is tiny.
Four, get three parts, Different sources, different year Poria cocos, carry out microscopic observation, observe microscopic features as shown in Figure 6 simultaneously:
The microscopic features that Poria cocos describes in " Chinese Pharmacopoeia " have following 2 (see figure 6)s:
1. powder chance chloral hydrate liquid clayization becomes gelatin, and heating agglomerate thing dissolves, and exposes mycelia.
2. hyphae colorless or be with brown (outer mycelia), more elongated, slightly bending, have branch, diameter 3 ~ 8 μm, minority to 16 μm, cross wall is accidental.
Five, get Different sources, the different year Radix Astragali three parts, carry out microscopic observation, observe microscopic features as shown in Figure 7 simultaneously:
The microscopic features that the Radix Astragali describes in " Chinese Pharmacopoeia " have following 3 (see figure 7)s:
1. fiber bunchy or fall apart from, diameter 8 ~ 30 μm, wall thickness, there is longitudinal crack on surface, and primary wall is often separated with secondary wall, and two ends normal off is cleaved into must shape, or more truncate.
2. bordered pit vessel is colourless or orange-yellow, and bordered pit arrangement closely.
3. lithocyte is rare, and circle, Long Circle or out-of-shape, wall is thicker.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 3 (see figure 7)s:
4. amylum body is more.Simple grain similar round, ellipse or class kidney shape, diameter 3 ~ 13 μm; Composite grain is made up of 2 ~ 4 gradation.
5. cork cell's yellowish green.It is that class polygon or class are square that surface is seen, and anticline is thin, and what have is thin wavy bending.
6. prothenchyma (of wood) is rare.In class triangle or class square, diameter about to 60 μm, wall thickness to 10 μm, micro-lignify, laminated striation is visible, and hole ditch is rare.
Six, get Different sources, the different year banksia rose three parts, carry out microscopic observation, observe microscopic features as shown in Figure 8 simultaneously:
The microscopic features that the banksia rose describes in " Chinese Pharmacopoeia " have following 4 (see figure 8)s:
1. synanthrin is common, the existing radial texture in surface.
2. the many bunchys of xylogen, spindle shape, diameter 16 ~ 24 μm, pit aperture transverse fissure gap-like, crosswise or people's shape.
3. reticulate vessel is common, also has bordered pit vessel, diameter 30 ~ 90 μm.
4. grease chamber's fragment is sometimes visible, includes yellow or brown secretion.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 2 (see figure 8)s:
5. cork cell is fallow.Surface is seen in class polygon, not of uniform size, and arrangement is very not neat, and the wavy that anticline has bends.
6. parenchyma cell light brown or fallow.Containing little shape calcium oxalate prismatic crystal in some cells, or containing volatilization oil droplet.In addition, irregular brown block is had to be dispersed in.
Seven, get Different sources, different year ginseng three parts, carry out microscopic observation, observe microscopic features as shown in Figure 9 simultaneously:
The microscopic features that ginseng describes in " Chinese Pharmacopoeia " have following 5 (see figure 9)s:
1. resin canal fragment is easily shown in, containing yellow block secretion.
2. calcium oxalate cluster crystal diameter 20 ~ 68 μm, the sharp point of corner angle.
3. the square or polygon of class is seen on cork cell surface, and wall is thin wavy bending.
4. reticulate vessel and scalariform duct diameter 10 ~ 56 μm.
5. amylum body is a lot of, and simple grain class is spherical, semicircle or irregular polygon, diameter 4 ~ 20 μm, omphalion point-like or slit-like; Composite grain is made up of 2 ~ 6 gradation.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 1 (see figure 9):
6. wood-parenchymatous cell be rectangle or class square, wall is thin, surface accidental fine oblique staggered texture.
Eight, get Different sources, different year fructus amomi three parts, carry out microscopic observation, observe microscopic features as shown in Figure 10 simultaneously:
The microscopic features that fructus amomi describes in " Chinese Pharmacopoeia " have following 7 (see figure 10)s:
1. endotesta prothenchyma (of wood) rufous or yellowish-brown, polygon is seen on surface, wall thickness, non-lignify, and cell includes siliceous piece; It is 1 row palisade cells that section is seen, and inwall and sidewall are extremely thick, and the inclined outside of cell, includes siliceous piece.
2. seed coat epidermal cell is faint yellow, and strip is seen on surface, normal and hypoblast levels homeotropic alignment.
3. hypoblast is containing brown or rufous thing.
4. uvea cell shrinkage, boundary is unclear, containing rufous or dark-brown thing.
5. perisperm cell rectangle like or irregular shape, is full of the starch group that tiny amylum body is combined into, and what have is embedded with tiny calcium oxalate prismatic crystal.
6. endosperm cell is containing tiny aleurone grain and fatty oil droplet.
7. eleocyte is colourless, and wall is thin, and accidental oil droplet is dispersed in.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 3 (see figure 10)s:
8. calcium oxalate cluster crystal is present in chromatophore or aril cell, diameter about to 30 μm.
9. aril cell is long and narrow, and wall is thin, shrinkage, and what have contains prismatic crystal and cluster crystal.
10. pigment block rufous or brown, not of uniform size, is dispersed in.
Nine, get Different sources, different year Chinese yam three parts, carry out microscopic observation, observe microscopic features as shown in figure 11 simultaneously:
Chinese yam describes microscopic features in " Chinese Pharmacopoeia " to be had following 3 (see Figure 11):
1. amylum body simple grain flat avette, triangular shape is avette, similar round or square circular, diameter 8 ~ 35 μm, omphalion point-like, people's shape, crosswise or short gap-like, visible laminated striation; Composite grain is rare, is made up of 2 ~ 3 gradation.
2. needle-like calcium oxalate crystal bundle is present in mucilage cell, is about to 240 μm, thick 2 ~ 5 μm of needle.
3. bordered pit vessel, reticulate vessel, spiral duct and annular duct diameter 12 ~ 48 μm.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 2 (see Figure 11):
4. screen casing is adjacent to conduit.Sieve tube element end wall tool compound sieve plate, has most sieve area, makes meshy arrangement.
5. fiber (outer skin zone) minority.Elongated, diameter about 14 μm, wall is very thick, lignify, and hole ditch is not obvious, and cell is linear.In addition, parenchymal tissue is more, and cell is similar round.
Ten, get Different sources, different year wild jujube three parts, carry out microscopic observation, observe microscopic features as shown in figure 12 simultaneously:
The microscopic features that wild jujube describes in " Chinese Pharmacopoeia " have following 3 (see Figure 12):
1. seed coat palisade cells brownish red, polygon is seen on surface, diameter about 15 μm, wall thickness, lignify, and cell is little; Side is seen elongated, outer wall thickening, and on sidewall, middle part is very thick, and bottom is gradually thin; Antapical view class polygon or circle polygon.
2. seed coat endepidermis cell brown color, surface see rectangle or class square, anticline beaded thickens, lignify.
3. cotyledon epidermal cell is containing tiny calcium oxalate cluster crystal and prismatic crystal.
Observed by a large amount of microscope experiment, in addition to those described above, newly-increased microscopic features 3 (see Figure 12):
4. brown parenchyma cell cell boundary is often unclear, containing light brown or yellowish-brown thing.
5. endosperm and cotyledon cell are all containing aleurone grain, diameter 2 ~ 10 μm, the square or polygonal crystalloid of visible small class; And fatty oil droplet.
6. conduit spiral duct diameter 5 ~ 9 μm.
11, get Different sources, different year polygala root three parts, carry out microscopic observation, observe microscopic features as shown in fig. 13 that simultaneously:
Polygala root in " Chinese Pharmacopoeia " without micro-description.
Observed by a large amount of microscope experiment, newly-increased microscopic features 6 (see Figure 13):
1. calcium oxalate cluster crystal is present in parenchyma cell or is dispersed in.Diameter 14 ~ 55 μm, corner angle are wider and thin, and tip is mostly more truncate.
2. cork cell is faint yellow or several colourless.Section is seen and is rectangle; Surface is seen in class polygon or long polygon, and anticline is thinner, micro-lignify, has pit to be interrupted shape.
3. parenchyma cell similar round, fatty oil droplet; Accidental containing calcium oxalate cluster crystal.
4. xylogen is single is dispersed in or bunchy, elongated, diameter 9 ~ 22 μm, micro-lignify.Libriform fibre wall thickness 3 ~ 7 μm, outer wall is slightly in serration shape, and some walls are more smooth, and hole ditch is rare; The bordered pit of fibre tracheid is obvious, arranged askew.
5. conduit master is bordered pit vessel, also has tiny reticulate pattern or spiral duct, diameter 7 ~ 40 μm.
6. wood-parenchymatous cell is rectangle like, and wall thickness about 4 μm, lignify, has pit, and hole ditch is obvious.
The microscopic features figure of ginseng spleen-strengthening bolus Chinese patent drug reference substance is provided below:
Ginseng spleen-strengthening bolus Chinese patent drug describes microscopic features 3 (see Figure 14 ~ 16) in " Chinese Pharmacopoeia ":
1, needle-like calcium oxalate crystal bundle is present in mucilage cell, long 80 ~ 240 μm, needle diameter 2 ~ 5 μm (Chinese yam).
2, calcium oxalate cluster crystal diameter 20 ~ 68 μm, the sharp point of corner angle (ginseng).
3, endotesta prothenchyma (of wood) yellowish-brown or brownish red, class polygon is seen on surface, wall thickness, and cell is containing siliceous piece (fructus amomi).
Observed by a large amount of microscope experiment, apart from the above description, newly-increased microscopic features 8 (see Figure 17 ~ 24):
1, needle-like calcium oxalate crystal is tiny, long 10 ~ 32 μm, is filled in brokenly (bighead atractylodes rhizome) in parenchyma cell.
2, parenchyma cell spindle, wall is slightly thick, has atomic thin cross lamination (Radix Angelicae Sinensis).
3, irregular branch agglomerate is colourless, meets chloral hydrate liquid and dissolves; Hyphae colorless or light brown (Poria cocos).
4, calcium oxalate prismatic crystal is present in (dried orange peel) in parenchyma cell in flakes.
5, xylogen bunchy, spindle shape, diameter 16 ~ 24 μm, wall is slightly thick, pit aperture transverse fissure gap-like, crosswise or people's shape; Reticulate vessel diameter about to 90 μm.(banksia rose).
6, fiber bunchy or fall apart from, wall thickness, there is longitudinal crack on surface, and two ends fragment into broom shape or more truncate (astragalus root).
7, cork cell surface is seen in polygon. and the square or rectangle like of class, anticline is thinner, has pit, in interrupted shape (polygala root).
8, endotesta cell brown color, surface see rectangle or class square, anticline beaded thickens (wild jujube).
Claims (2)
1. a method for ginseng spleen-strengthening bolus Microscopic Identification, is characterized in that concrete steps are as follows:
One, ginseng spleen-strengthening bolus raw medicinal material and the micro-collection of illustrative plates of Chinese patent drug reference substance and cell tissue database thereof is set up:
Microscopic features 64 are had in the micro-collection of illustrative plates of described ginseng spleen-strengthening bolus raw medicinal material reference substance and cell tissue database thereof, comprising the microscopic features 40 in " Chinese Pharmacopoeia " version in 2010, the microscopic features newly increased 24;
Total microscopic features 11 in the database of the micro-collection of illustrative plates of described ginseng spleen-strengthening bolus Chinese patent drug reference substance and cell tissue thereof, comprising the microscopic features 3 in " Chinese Pharmacopoeia " version in 2010, the microscopic features newly increased 8;
(1) ginseng spleen-strengthening bolus raw medicinal material and the film-making of ginseng spleen-strengthening bolus Chinese patent drug reference substance:
(1) powder film-making: reference substance powder is crossed No. five sieves, picking is put on microslide a little, drips glycerine acetic acid test solution or chloral hydrate test solution, covered; If desired, heat penetration is added;
(2) ginseng spleen-strengthening bolus Chinese patent drug reference substance microsection: get the ginseng spleen-strengthening bolus water-bindered pill, water-honeyed pill, large honeyed bolus and each several ball of small honey pill, put pulverize in mortar respectively, get appropriate powder; Or honeyed bolus is cut, from tangent plane by central picking appropriate amount of sample or after taking off honey with water, absorption sediment is a small amount of; By the film-making of above-mentioned powder method of tableting;
(2) micrometering is carried out to the micro-collection of illustrative plates of described reference substance:
Micrometering refers to the size measuring cell and ergastic mateiral under the microscope with eyepiece micrometer; First by eyepiece micrometer microscope carrier micrometer footage number, calculate μm number of each little lattice, will the little lattice number of object be recorded during application, and be multiplied by μm number of each little lattice, obtain the size of predicted earnest;
(3) the micro-collection of illustrative plates of described reference substance is observed and imaging:
" microscope image pick-up with the automated imaging of display and memory storage " is adopted to carry out microexamination;
First microsection is placed on microscopical objective table during microexamination, micro-image candid photograph is carried out to the cell observed, tissue structure shape and content shape, and stored in corresponding database; Next measures the size of cell, tissue, is placed in by microsection on microscopical objective table, by eyepiece micrometer and objective table micrometer, measures maximal value and the minimum value of cell, tissue structure shape and content geomery; During observation, first using 10 times of object lens to observe with Nosepiece, when observing object, rotating Nosepiece, use 40 times of object lens to aim at it and measure observation, then take, Image Saving in specified database; During microscopy, described reference substance microslide first should move along X-axis and observe, then moves along Y-axis and observe, in case omit;
Two, by the method identical with above-mentioned steps one, the candid photograph of micro-collection of illustrative plates, observation, measurement cell tissue feature are carried out to test specimen microsection;
The micro-collection of illustrative plates of micro-for test specimen collection of illustrative plates and reference substance and cell tissue color, form, each feature of texture are carried out contrast and analysis, draws qualification result;
Described 24 microscopic features newly increased are as follows:
(1) bighead atractylodes rhizome increases by 2, i.e. cork cell and test-tube baby;
(2) Radix Angelicae Sinensis increases by 2, i.e. cork cell and amylum body;
(3) Radix Astragali increases by 3, i.e. amylum body, cork cell and prothenchyma (of wood);
(4) banksia rose increases by 2, i.e. cork cell and parenchyma cell;
(5) ginseng increases by 1, i.e. wood-parenchymatous cell;
(6) fructus amomi increases by 3, i.e. cluster crystal, aril cell and pigment block;
(7) Chinese yam increases by 2, i.e. fiber and screen casing;
(8) wild jujube increases by 3, i.e. brown parenchyma cell, endosperm and cotyledon cell and conduit;
(9) polygala root increases by 6, namely calcium oxalate cluster crystal, cork cell, parenchyma cell, xylogen single be dispersed in or bunchy, conduit, wood-parenchymatous cell;
The collection process of described microscopic features is as follows:
(1) micro-collection of illustrative plates is collected;
(2) micrograph spectrum information is read;
(3) microscopic features are extracted;
Described microscopic features comprise color characteristic, contour feature, textural characteristics and length characteristic;
(4) by above-mentioned microscopic features stored in described database;
The micro-collection of illustrative plates of test specimen and reference substance micro-collection of illustrative plates comparative analysis process as follows:
(1) test specimen proper vector is obtained:
1. micro-for test specimen collection of illustrative plates is extracted the region that will contrast through Image semantic classification;
2. feature extraction is carried out;
3. test specimen proper vector is obtained;
(2) reference substance proper vector stored in reference substance feature database is obtained:
1. the micro-collection of illustrative plates in reference substance micrograph modal data storehouse is carried out Image semantic classification and extract the region that will contrast;
2. feature extraction is carried out;
3. reference substance proper vector stored in reference substance feature database is obtained;
(3) test specimen proper vector is carried out characteristic matching to the corresponding reference substance proper vector in reference substance feature database.
2. the method for a kind of ginseng spleen-strengthening bolus Microscopic Identification according to claim 1, is characterized in that 8 microscopic features that described ginseng spleen-strengthening bolus Chinese patent drug newly increases are as follows:
(1) for the bighead atractylodes rhizome in ginseng spleen-strengthening bolus: needle-like calcium oxalate crystal is tiny, long 10 ~ 32 μm, be filled in brokenly in parenchyma cell;
(2) for the Radix Angelicae Sinensis in ginseng spleen-strengthening bolus: parenchyma cell spindle, wall is slightly thick, has atomic thin cross lamination;
(3) for the Poria cocos in ginseng spleen-strengthening bolus: irregular branch agglomerate is colourless, meet chloral hydrate liquid and dissolve; Hyphae colorless or light brown;
(4) for the dried orange peel in ginseng spleen-strengthening bolus: calcium oxalate prismatic crystal is present in parenchyma cell in flakes;
(5) for the banksia rose in ginseng spleen-strengthening bolus: xylogen bunchy, spindle shape, diameter 16 ~ 24 μm, wall is slightly thick, pit aperture transverse fissure gap-like, crosswise or people's shape; Reticulate vessel diameter about to 90 μm;
(6) for the astragalus root in ginseng spleen-strengthening bolus: fiber bunchy or fall apart from, wall thickness, there is longitudinal crack on surface, and two ends fragment into broom shape or more truncate;
(7) for the polygala root in ginseng spleen-strengthening bolus: cork cell sees in polygon on surface. the square or rectangle like of class, anticline is thinner, has pit, in interrupted shape;
(8) for the wild jujube in ginseng spleen-strengthening bolus: endotesta cell brown color, surface see rectangle or class square, anticline beaded thickens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310072495.4A CN103134801B (en) | 2013-03-07 | 2013-03-07 | Microscopic identification method for ginseng spleen-strengthening pills |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310072495.4A CN103134801B (en) | 2013-03-07 | 2013-03-07 | Microscopic identification method for ginseng spleen-strengthening pills |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103134801A CN103134801A (en) | 2013-06-05 |
| CN103134801B true CN103134801B (en) | 2015-07-15 |
Family
ID=48494911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310072495.4A Active CN103134801B (en) | 2013-03-07 | 2013-03-07 | Microscopic identification method for ginseng spleen-strengthening pills |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103134801B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103345720A (en) * | 2013-07-09 | 2013-10-09 | 康美药业股份有限公司 | Device and method for grade evaluation of traditional Chinese medicine decoction pieces |
| CN107356594A (en) * | 2017-05-24 | 2017-11-17 | 广东数相智能科技有限公司 | Medicinal material section detection method, electronic equipment and storage medium based on cell analysis |
| CN108226053A (en) * | 2017-12-22 | 2018-06-29 | 南京中医药大学 | Unstructured feature differentiation method based on multi-element intelligent fusion |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766655A (en) * | 2010-01-29 | 2010-07-07 | 药都制药集团股份有限公司 | Method for analyzing quality of Chinese medicinal materials, decoction pieces and Chinese patent drugs by utilizing micrographic atlases |
| CN202305986U (en) * | 2011-10-18 | 2012-07-04 | 药都制药集团股份有限公司 | Microscopic camera system for automatic image formation of microscopic image |
-
2013
- 2013-03-07 CN CN201310072495.4A patent/CN103134801B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103134801A (en) | 2013-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106990214B (en) | A method of evaluation Chinese medicine quality | |
| CN103134801B (en) | Microscopic identification method for ginseng spleen-strengthening pills | |
| CN101766655A (en) | Method for analyzing quality of Chinese medicinal materials, decoction pieces and Chinese patent drugs by utilizing micrographic atlases | |
| CN103149320B (en) | Quality control method of loins-strengthening and kidney-invigorating medicine | |
| CN101444589A (en) | Quality standard of Fenqing Wulin Wan and inspection method thereof | |
| CN102375000A (en) | Method for testing coconut island deer tortoise wine based on HNMR (hydrogen-nuclear magnetic resonance)-pattern recognition technique | |
| CN107356594A (en) | Medicinal material section detection method, electronic equipment and storage medium based on cell analysis | |
| CN102175707A (en) | New method for identifying and evaluating bulbus fritillariae cirrhosae | |
| CN102028859B (en) | Detection method for Chinese medicinal preparation for treating asthma | |
| CN102042978B (en) | Colorimetric card for quickly judging grades of Chinese medicinal materials and preparation method thereof | |
| CN103940738A (en) | Method for quickly identifying ginseng age | |
| Yu et al. | Microscopic research on a multi-source traditional Chinese medicine, Astragali Radix | |
| CN108152245A (en) | A kind of discrimination method of roxburgh anoectochilus terminal bud and its mixed adulterant | |
| CN103983492A (en) | Jade nondestructive detection sample model and manufacturing method thereof | |
| CN110501433A (en) | A kind of discrimination method of the callicarpa nudiflora medicinal material of different sources | |
| CN102279185A (en) | Method for identifying years of American ginseng medicinal materials | |
| CN103163272B (en) | Quality control method of infant spleen tonifying medicament | |
| Au et al. | A novel method to identify the Chinese herbal medicine Wuzhimaotao by quantification of laticifers | |
| CN101491630A (en) | Quality control method of Fenqing Wulin pill | |
| CN110596297B (en) | Quality standard detection method for king melon seed medicinal material | |
| CN101403738A (en) | Measuring method for wall breaking rate of traditional Chinese medicine micro mist cell | |
| CN105510330B (en) | A method of distinguishing Guizhou Province fine jade germplasm Blumea balsamifera | |
| Zhao et al. | Compare the microscopic characteristics of stems of the 24 Dendrobium species utilized in the traditional Chinese medicine “Shihu” | |
| CN101698034B (en) | Method for controlling quality of modified ageratum pills | |
| CN207692735U (en) | A kind of Planktont counting disk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: ANGUO TIANEN MEDICINAL PRODUCTS INSPECTION LIMITED Effective date: 20150608 Owner name: YAODU PHARMACEUTICAL GROUP CO., LTD. Free format text: FORMER OWNER: ANGUO TIANEN MEDICINAL PRODUCTS INSPECTION LIMITED Effective date: 20150608 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20150608 Address after: 071200 Hebei city in Baoding Province, Anguo City, Oriental medicine city abou northbound Applicant after: Yaodu Pharmaceutical Group Co., Ltd. Applicant after: Anguo Tianen Medicinal Products Inspection Limited Address before: 071200 Hebei city in Baoding Province, Anguo City, Oriental medicine city abou northbound Applicant before: Anguo Tianen Medicinal Products Inspection Limited |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |