CN103116011B - Method for qualitatively comparing contents of NOBs (Nitrite-Oxidizing Bacteria) in different kinds of nitrosation sludge - Google Patents
Method for qualitatively comparing contents of NOBs (Nitrite-Oxidizing Bacteria) in different kinds of nitrosation sludge Download PDFInfo
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- CN103116011B CN103116011B CN201310005457.7A CN201310005457A CN103116011B CN 103116011 B CN103116011 B CN 103116011B CN 201310005457 A CN201310005457 A CN 201310005457A CN 103116011 B CN103116011 B CN 103116011B
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Abstract
The invention discloses a method for qualitatively comparing contents of NOBs (Nitrite-Oxidizing Bacteria) in different kinds of nitrosation sludge. The method is characterized by comprising the following steps of: placing the nitrosation sludge into an SBR (Sequencing Batch Reactor), controlling the dissolved oxygen to be 6.0-8.0mg/L, carrying out continuous aeration, then sampling every other a period of time, measuring 3-nitrogen concentration until NO2<->-N in the reactor is completely oxidized into NO3<->-N, recording the reaction time, and finally, calculating the NO3<->-N generation rate, namely, the differential coefficient of the quantity of the NO3<->-N converted by the sludge of unit mass to the time, thus obtaining the content of the NOBs in the nitrosation sludge by qualitatively comparing according to the principle that the NO3<->-N generation rate is directly proportional with the NOB in the nitrosation sludge. According to the invention, the problems of the inhibition of the NOB in the nitrosation sludge and undefined elutriation degree existing for a long time are solved, the performance of the nitrosation sludge is completely known, and the firm foundation is laid for the later operation strategy.
Description
Technical field
The invention belongs to municipal sewage treatment and resource field.Be specifically related to the method for NOB content in the different nitrosation mud of a kind of qualitative contrast.
Background technology
Along with the quickening of China's process of industrialization, physical environment especially water environment has suffered more serious destruction, and rivers and storehouse, lake quality of water environment worsen increasingly.From last century the eighties, country accelerates the paces to water environment treatment, and wastewater treatment rate is greatly improved, but pollutes by nitrogen and phosphorus the body eutrophication problem caused and not only do not solve, and has day by day serious trend.
Traditional biological denitrification process is, by amonifying bacteria, the organic nitrogen in water is converted into ammonia nitrogen, is then nitrate nitrogen by nitration reaction by mineralized nitrogen, then is that nitrogen is overflowed from water by denitrification by nitrate-nitrogen reduction.But, traditional nitrated/denitrification biological denitrogenation technique exist long flow path, oxygen-supplying amount high, need the problems such as additional carbon, sludge yield are high, above problem will increase capital expenditure and operating cost greatly.
Therefore, with short distance nitration (NH
4 +-NO
2 -) become the study hotspot of current nitrogenous effluent treatment, such as short-cut nitrification and denitrification technology, simultaneous nitrification-denitrification technology, short distance nitration-anaerobic ammoxidation technology etc. for the short-cut denitrification technology of core.Short-cut nitrification technology is at NO nitration reaction process control
2 -generation phase, stops NO
2 -be further oxided as NO
3 -.On this stage theory comparable traditional complete nitrification technology reduce the oxygen demand of 25%, the carbon source of 40% and 50% sludge yield.The bacterium participating in this process has ammonia oxidation bacteria (ammonia-oxidizing bacteria is called for short AOB) and NOB (nitrite-oxidizing bacteria is called for short NOB) two kinds of different autotrophic bacteriums.This two classes bacterium utilizes inorganic carbide as CO
3 2-, HCO
3 -and CO
2make carbon source, from NH
3, NH
4 +or NO
2 -oxidation reaction in obtain energy.Because the growth characteristics of this two bacterioid there are differences, different to the sensitivity of change in reaction conditions, therefore can screen a wherein bacterioid by controlling reaction conditions.
Chinese scholars has carried out a large amount of technical research to Short-Cut Nitrification Process, and achieves certain achievement in research and practical experience, and analyzes the controlling elements of nitrosation reaction and influence factor.Show that the content of NOB in nitrosation mud is how many by research and have decisive role to later stage nitrosifying long-term stability operation, if the content of NOB is more in nitrosation mud, be then unfavorable for that later stage nitrosifying long-term stability is run.If only intuitively by words that this Common Parameters of nitrosoation rate compares, often can not draw correct conclusion, because in nitrosation reaction process, some reaction conditions of usual control suppresses the activity of NOB, but As time goes on, NOB can adapt to these rejection conditions, thus shows activity.Therefore, in mud, the more few later stage nitrosifying long-term stability that is more beneficial to of the content of NOB is run.
So, find the content that a kind of simple and effective assay method carrys out NOB in the different nitrosation mud of qualitative contrast, sludge character can be understood in time, formulate the strategy suppressing and eluriate NOB in time and ensure that nitrosifying long-term stability is run, there is important engineering significance and scientific research value.
Summary of the invention
The object of the present invention is to provide the method for NOB content in the different nitrosation mud of a kind of qualitative contrast.
The invention provides and a kind ofly measure sub-nitrogen by extended aeration and convert the method that nitre nitrogen generating rate (amount of the nitre nitrogen that unit mass mud transforms is to the derivative of time) in nitre nitrogen process carrys out NOB content in the different nitrosation mud of qualitative contrast to, the nitre nitrogen generating rate utilizing sub-nitrogen to be oxidized in nitre nitrogen process is how many to the content contrasting NOB in different nitrosation mud from the principle that the content of NOB in nitrosation mud is directly proportional, and it is characterized in that:
Get nitrosation mud and put into sbr reactor device, control dissolved oxygen DO is 6.0-8.0mg/L, carries out continuous aeration.Then three nitrogen concentration mensuration are carried out every one section of sampling, until reactor Central Asia nitrogen is all oxidized to nitre nitrogen, recording reacting time, finally calculate nitre nitrogen generating rate, namely the amount of nitre nitrogen that transforms of unit mass mud is to the derivative of time, the principle be directly proportional to the content of NOB in nitrosation mud according to nitre nitrogen generating rate and then the qualitative content contrasting NOB in nitrosation mud.
If the nitre nitrogen generating rate calculating gained last is larger, then prove that in this kind of nitrosation mud, NOB content is higher; Otherwise the content of NOB is lower in this kind of nitrosation mud.
Compared with classic method, advantage of the present invention is:
The first, in the different nitrosation mud of the current qualitative contrast in laboratory, the method for NOB content mostly adopts Experiment on Microbiology, comprises the method such as FISH, DGGE, fluorescent quantitative PCR, and equipment is complicated, expensive, complicated operation.And this method is simple, efficient, easy to learn, there are great directive significance and indicative function for later stage nitrosifying stable operation.
Second, in assay method of the present invention, water quality and operational factor, sample time need setting voluntarily according to mensuration person, there is great dirigibility, the various nitrosation mud of different quality, different operation conditions, different reactor type can be adapted to, be with a wide range of applications.
Below in conjunction with embodiment, the invention will be further described, but protection scope of the present invention is not limited thereto.
Accompanying drawing explanation
Fig. 1 is that the nitre nitrogen generating rate of nitrosation mud A, B is schemed over time.
Fig. 2 is that the nitre nitrogen generating rate of nitrosation mud C, D is schemed over time.
Embodiment
Concrete steps are as follows:
Step 1: build sbr reactor device, seed sludge
Nitrosation mud is dropped into respectively in all identical sbr reactor device of size shape.
Step 2: select operational factor
Pass into water in reactor, the operational factors such as selective temperature, aeration rate, dissolved oxygen DO, stirring extent, but need ensure that the service condition of carrying out the reactor contrasted is identical.
Step 3: operational process
After determining various parameter, continuous aeration is carried out to sbr reactor device, samples at set intervals, measure three nitrogen concentrations, exist until can't detect sub-nitrogen, recording reacting time, stop aeration.
Step 4: analytical parameters
Calculate the nitre nitrogen generating rate in process of the test.Nitre nitrogen generating rate is faster, and illustrate that the content of NOB in nitrosation mud is higher, this kind of mud is more unfavorable for the stable operation in later stage; Otherwise the content of NOB is lower in nitrosation mud, advantageously in the nitrosation stable operation in later stage.
Step 5: repeat step 1 to the content of step 4, carry out revision test, to eliminate the impact of accidentalia and error.
Test is with water based on certain community life sewage, and concrete water quality is as follows: COD=300-400mg/L, BOD
5=120-150mg/L, SS=76-114 mg/L, TP=5.75-6.30mg/L, NH
4 +-N=75-90mg/L, NO
2 --N≤0.25mg/L, NO
3 --N≤1.5mg/L, TP≤1mg/L, pH=7.0-7.8, basicity is that 550.59-610.78mg/L(is with CaCO
3meter).
Concrete process is as follows:
Example 1
The content of NOB in the nitrosation mud run under more different DO concentration (0.5-1.0mg/L, 2.5-3.0mg/L).
Nitrosation mud A: inoculate high ammonia nitrogen start the nitrosation mud of nitrosoation rate more than 95%, DO be run 60d under the condition of 0.5-1.0mg/L after obtain nitrosation mud A.
Nitrosation mud B: originally seed sludge is identical with A, DO be run 60d under the condition of 2.5-3.0mg/L after obtain nitrosation mud B.
Get nitrosation mud A, B (nitrosoation rate of A, B nitrosation mud is more than 90%) of identical amount, be placed in 1#, 2#SBR reactor that useful volume is 1L respectively, pass into actual domestic wastewater, carry out aeration agitation, maintenance DO is 6.0-8.0mg/L.Sampled after one hour once, measure three nitrogen concentrations.First ammonia nitrogen is all oxidized to sub-nitrogen by AOB, afterwards due to extended aeration act on the effect of NOB under sub-nitrogen be oxidized to nitre nitrogen gradually.When mensuration water sample Central Asia nitrogen concentration is 0, stop aeration, off-test.In order to reduce the error in the contingency that exists in process of the test and mensuration process, again getting nitrosation mud A, B of identical amount, carrying out revision test.After off-test, the mean value getting each sample is analyzed, and calculates the derivative to the amount time of the nitre nitrogen that unit mass mud transforms, i.e. nitre nitrogen generating rate, and contrasts.
The nitre nitrogen generating rate of nitrosation mud A, B is schemed as shown in Figure 1 over time.As can be seen from the figure the nitre nitrogen generating rate of nitrosation mud B is larger than A, illustrates that the content of NOB in mud B is more, is unfavorable for that later stage nitrosifying long-term stability is run.
Example 2
The relatively content of NOB in nitrosation mud under two kinds of different running method (continuous aeration, intermittent aerating).
Nitrosation mud C: inoculate the nitrosation mud of nitrosoation rate more than 95% that high ammonia nitrogen starts, obtain nitrosation mud C run 90d under the condition of continuous aeration after.
Nitrosation mud D: originally seed sludge is identical with C, under the condition of intermittent aerating, (aeration/stop the time of exposing to the sun be 30min/10min) obtains nitrosation mud D after running 90d.
Get nitrosation mud C, D (nitrosoation rate of C, D nitrosation mud is more than 90%) of identical amount, be placed in 3#, 4#SBR reactor that useful volume is 1L respectively, pass into actual domestic wastewater, carry out aeration agitation, maintenance DO is 6.0-8.0mg/L.Sampled after one hour once, measure three nitrogen concentrations.First ammonia nitrogen is all oxidized to sub-nitrogen by AOB, afterwards due to extended aeration act on the effect of NOB under sub-nitrogen be oxidized to nitre nitrogen gradually.When mensuration water sample Central Asia nitrogen concentration is 0, stop aeration, off-test.In order to reduce the error in the contingency that exists in process of the test and mensuration process, again getting nitrosation mud C, D of identical amount, carrying out revision test.After off-test, the mean value getting each sample is analyzed, and calculates the amount of the nitre nitrogen that unit mass mud transforms to the derivative of time, i.e. nitre nitrogen generating rate, and contrasts.
The nitre nitrogen generating rate of nitrosation mud C, D is schemed as shown in Figure 2 over time.As can be seen from the figure the nitre nitrogen generating rate of nitrosation mud C is larger than D, illustrates that the content of NOB in mud C is more, is unfavorable for that later stage nitrosifying long-term stability is run.
Claims (1)
1. the method for NOB content in the different nitrosation mud of qualitative contrast, is characterized in that:
Get nitrosation mud and put into sbr reactor device, control dissolved oxygen DO is 6.0-8.0mg/L, carries out continuous aeration; Then three nitrogen concentration mensuration are carried out every one section of sampling, until reactor Central Asia nitrogen is all oxidized to nitre nitrogen, recording reacting time, finally calculate nitre nitrogen generating rate, namely the amount of nitre nitrogen that transforms of unit mass mud is to the derivative of time, the principle be directly proportional to the content of NOB in nitrosation mud according to nitre nitrogen generating rate and then the qualitative content contrasting NOB in nitrosation mud.
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2013
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| JPS5370554A (en) * | 1976-12-07 | 1978-06-23 | Ebara Infilco Co Ltd | Method of biologically denitrifying organic waste water |
| JP2000206087A (en) * | 1999-01-13 | 2000-07-28 | Fuji Electric Co Ltd | Method and device for detecting nitrification-blocking substance in sewage and discharged water |
| CN1363525A (en) * | 2002-01-25 | 2002-08-14 | 浙江大学 | Process for bio-denitrifying sewage |
| EP1595852A4 (en) * | 2003-02-21 | 2009-12-16 | Kurita Water Ind Ltd | Method for treating water containing ammonia nitrogen |
| CN101419173A (en) * | 2008-11-28 | 2009-04-29 | 北京工业大学 | Method for synchronistically detecting triaza concentration in water |
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