CN103114100B - Beta-glucosaccharase gene S-bgl3 and application thereof - Google Patents
Beta-glucosaccharase gene S-bgl3 and application thereof Download PDFInfo
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Abstract
The invention provides a Beta-glucosaccharase gene S-bgl3 and application of the Beta-glucosaccharase gene S-bgl3. Beta-glucosaccharase gene S-bgl3 has the nucleotide sequence as shown in SEQ ID NO: 1; and the genetically coded Beta-glucosaccharase S-bgl3 has the amino acid sequence as shown in SEQ ID NO: 2. The Beta-glucosaccharase S-bgl3 can be applied to the decomposition of cellobiose.
Description
Technical field
The present invention relates to a beta-glucosidase gene S-bgl3 and application thereof, the protein of this genes encoding can be used for degradation of fibers disaccharides.
Background technology
Beta-glucosidase (beta-glucosidase, EC3.2.1.21) claims again β-D-Glucose glycosides glucose hydrolysis enzyme, is a kind of in cellulase system.Beta-glucosidase has important value (Ramakrishnan Anish aspect the sugar metabolism of the mankind, animal, plant and microorganism, Mohammad Safikur Rahman, and Mala Rao, 2007, Application of cellulase from an alkalothermophilic Thermomonospora sp.in biopolishing of denims, Biotechnol.Bioeng., 96 (1): 48-56.).
Mierocrystalline cellulose, as the main polyose product of photosynthesis of plant, is the abundantest carbohydrate of content on the earth, is also a kind of renewable resources of quantity maximum.It is reported, the Mierocrystalline cellulose that the whole world produces by photosynthesis is every year up to 1.55 * 10
9t, wherein 85% not yet by human use (Dunlap C., Chiang G.C., 1980, Utilization and recycle of agriculture wastes and residues.Shuler M L.BocaRaton, Florida.USA:CRC Press Inc, 19.).Now, energy shortage is one of significant problem of facing mankind.And for cellulosic utilization, be the important channel that solves energy dilemma, food shortage, environmental pollution.Beta-glucosidase has vital role (Sestelo A.B.F. in cellulose degradation, Poza M., Villa T.C.2004, β-Glucosidase activity in a Lactobacillus plantarum wine strain.World Journalof Microbiology & Biotechnology, 20:633～637.).Beta-glucosidase hydrolysis fiber disaccharides generates two molecule glucoses, it is the collaborative key enzyme of doing of cellulase prozyme system performance, integral hydrolysis activity to cellulase system has very strong promoter action, can improve by improving the activity of beta-glucosidase in cellulase system function (the Bhat K.M. of whole cellulase system, Bhat S., 1997, Cellulose degrading enzymes and their potential industrial applications.Biotechnol.Adv., 15:583～620.), therefore the further investigation of beta-glucosidase being subject to people more and more pays close attention to.
Except important role aspect degraded cellulose, beta-glucosidase also has a wide range of applications at other field, particularly in medicine, food, bio-transformation, there is important using value (Michael E.H., Mark F.R., Charles E., 1999, Cellulase for commodity products from cellulosic biomass.Curr.Opin.Biotechnol., 10 (4): 358～364.).In medical science, beta-glucoside endonuclease capable be applied in some cancer diagnosis and treatment in, and obtained many successes (Shao Jinhui, Han Jinxiang, etc., 2005, beta-glucosidase is cured the application in field workers and peasants. the chemistry of life, 5(01): 22-24.).In the foodstuffs such as fruit, vegetables, tealeaves and herbal medicine, beta-glucoside endonuclease capable is as food flavor enzyme, improvement good flavor, fruit wine flavouring and tealeaves flavouring all have highly significant effect (Li Yuan China .2002, the progress of beta-glucosidase. the journal .29 of Agricultural University Of Anhui (4): 421-425.).Many flavor precursors and pharmacological component mainly exist with glucosides form, need this food flavor enzyme of beta-glucosidase to be discharged volatility aglycone, play flavouring effect (Jin Fengxie, village's fine jade, the red sudden strain of a muscle of fish, etc., 2009, special herbal medicine glycoside glycosides enzyme microorganism and fermentation and enzymatic property. biotechnology journal, 25 (12): 1863-1870.).In addition, beta-glucosidase also has the activity that shifts glucosyl group, can be used for producing oligomeric dragon gallbladder sugar (Yongling Qin, Yunkai Zhang, Haiyan He, et al, 2011, Screening and identification of a fungal β-glucosidase and the enzymatic synthesis of gentiooligosaccharide.Appl Biochem Biotechnol.163 (8): 1012-9.), enzyme process synthesizes rhodioside (Wang Mengliang, Li Wanli .2009, immobilized β-glucosidase catalyzes and synthesizes the research of rhodioloside. biotechnology, 19 (1): 68-70.) and synthetic nonionogenic tenside alkyl glycoside (Zhu Yun, 2007, glucose enzyme process biosynthesizing alkyl glycoside technique. Zhejiang chemical industry, 38 (11): 3-6.).
The existing various beta-glucosidase genes from up to a hundred kinds microorganism, plant and animal-origins are cloned and are sequenced at present, and many microbe-derived beta-glucosidase genes have obtained heterogenous expression, and (Korea Spro laughs at, Chen Jienan, Wang Yiqiang, Deng, 2008, the cloning and expression progress of beta-glucosidase gene.Biotechnology circular, 3:8～13.).By contrast, the activity of beta-glucosidase of plant origin is low more than microbe-derived beta-glucosidase, therefore, current research is mainly to concentrate on microbe-derived upper (Shi Cairui, Wang Yiqiang, Chen Jienan, Deng, 2011, produce beta-glucosidase Microbial Breeding progress, biotechnology communication, 3:59-65.).And in microorganism, study more be that yeast, filamentous fungus are as (the Meng Xianwen such as bacillus in the moulds such as Trichoderma (Trichoderma), Aspergillus (Aspergillus) and bacterium, Song little Hong, Chen Lijun, Deng, 2009, the progress of beta-glucosidase. diary processing, 10:42-44.).But the cellulase activity that has multiple mycotoxins in Trichoderma tunning and obtain is lower, especially beta-glucoside enzyme activity is very low, cause cellobiose to accumulate in reaction system and affect enzymolysis efficiency, thereby its range of application is restricted, and (Korea Spro laughs at, Chen Jienan, Wang Yiqiang, 2008, the cloning and expression progress of beta-glucosidase gene.Biotechnology circular, 3:8～13.).
Take beta-glucosidase as keyword lookup State Intellectual Property Office patent retrieval database, occur altogether 164 patents of invention.Wherein have gene and application thereof that 30 patents of invention are description encoding beta-glucosidases, and other 134 is relevant with the application of beta-glucosidase on various fields.At these 30, there are 8 from the grand genomic library of various not culturing micro-organisms to the gene of encoding beta-glucosidase and the beta-glucosidase gene of applying in relevant patent of invention thereof, other 22 is different biological from 20 kinds, there is thermophilic deamination genus bacillus, Trichodermareesei, Agrobacterium tumefaciems, Saksenaea vasiformis XH8, Pyrococcus furiosus, thermophile bacteria, aspergillus niger, Duan Shi wood is mould, aspergillus niger, blackwing subterranean, Clostridium sp.WGC702, Aspergillus strain, thermophilic Paecilomyces varioti, Dictyoglomus thermophilum DSM3960, chaetomium thermophilum, yeast saccharomyces cerevisiae, Extreme basophilic bacteria, thermophilic Paecilomyces varioti, Ke Shi series bacillus and new sphingomonas bacteria, 1 from trans-resveratrol.
Summary of the invention
The genome of the streptomycete GX6 that the present invention screens from Nanning, be cloned into the gene S-bgl3 of an encoding beta-glucosidase, in e. coli host cell, express this genes produce beta-glucosidase S-bgl3, and can take cellobiose as raw material degraded generation glucose.
The present invention relates to the gene S-bgl3 of a beta-glucosidase, its nucleotide sequence as shown in SEQ ID NO:1, be from laboratory screening to the genome of streptomycete GX6 clone and obtain.The sequence of this beta-glucosidase gene S-bgl3 is by 1317 based compositions, the open reading frame that contains complete beta-glucosidase gene S-bgl3 (Open Reading Frame, ORF), the initiator codon of S-bgl3 gene is ATG, and terminator codon is TGA.
The protein of SEQ ID NO:2 is the beta-glucosidase product S-bgl3 of gene S-bgl3 coding, by 438 amino acid, formed, with S-bgl3 catalysis territory homology the highest be the beta-glucosidase(beta-glucosidase of Streptomyces coelicolor A3 (2) (sky blue streptomycete A3 (2))), both consistence=352/417 (84%), similarity=383/417 (91%).
Beta-glucosidase gene S-bgl3 can decompose cellobiose at the recombinant products S-bgl3 of expression in escherichia coli.
The invention still further relates to the expression vector that contains gene of the present invention, and for transforming the host of gene of the present invention.
The invention provides a beta-glucosidase gene S-bgl3, the beta-glucosidase S-bgl3 of this coded by said gene can apply in decomposing cellobiose.
Accompanying drawing explanation
Fig. 1 is that the Vitamin C2 that screening contains beta-glucosidase gene S-bgl3 recombinant bacterial strain is selected lithograph.
Fig. 2 is the SDS-PAGE figure of beta-glucosidase S-bgl3 purified.
Fig. 3 is the HPLC figure of beta-glucosidase S-bgl3 hydrolysis fiber disaccharides.
From Fig. 1, recognize, the recombinant bacterial strain that contains beta-glucosidase gene S-bgl3 can screen and on flat board, form black circle at Vitamin C2.
Fig. 2 recognizes, the molecular weight of beta-glucosidase S-bgl3 purified is 48kDa.
A in Fig. 3 and B are respectively the standard specimens of glucose and cellobiose.
From the C of Fig. 3, recognize, beta-glucosidase S-bgl3 can be hydrolyzed into glucose by cellobiose.
Embodiment
Following implementation method is in order better to explain the present invention, and the object should not be construed as limiting the invention.
Material used comprises in an embodiment of the present invention: intestinal bacteria (Escherichia coli) strain XL1-blue is purchased from Dalian TaKaRa company; Expression vector pSE380 is purchased from Stratagene company, and the reagent such as restriction enzyme, modifying enzyme are purchased from TaKaRa, MBI company.
Below will by embodiment, the present invention is described in detail:
1) extraction of streptomycete Streptomyces sp.GX6 genomic dna
The genomic dna of streptomycete Streptomyces sp.GX6 is the bacterial genomes DNA extraction test kit Biospin Bacteria Genomic DNA Extraction Kit(catalog number (Cat.No.) BSC12S1 according to BioFlux company) operation instruction extract.
Send Hua Da genome company to measure genome sequence the genomic dna of the streptomycete Streptomyces sp.GX6 having extracted.
2) analysis of encoding beta-glucosidase gene order in the genome sequence of streptomycete Streptomyces sp.GX6
By NCBI(National Center for Biotechnology Information for the genome sequence of the streptomycete Streptomyces sp.GX6 obtaining,
http:// www.ncbi.nlmsoftware ORF finder .nih.gov) (
http:// www.ncbi.nlm.nih.gov/gorf/orfig.cgi) and Blast (http://blast.ncbi.nlm.nih.gov/Blast.cgi) analyze.Result obtains altogether 8 and has the open reading frame (Open Reading Frame, ORF) of higher homology with beta-glucosidase, and the present invention only relates to one of them ORF.
3) nucleotide sequence analysis of beta-glucosidase gene S-bgl3
With NCBI(National Center for Biotechnology Information, http://www.ncbi.nlm.nih.gov) on software ORF finder (http://www.ncbi.nlm.nih.gov/gorf/orfig.cgi) and Blast (http://blast.ncbi.nlm.nih.gov/Blast.cgi) DNA sequence dna is analyzed.The open reading frame of gene S-bgl3 (Open Reading Frame, ORF) is comprised of 1317 Nucleotide, and sequence is as SEQ ID NO:1.Wherein, the initiator codon of S-bgl3 gene is ATG, and terminator codon is TGA.
4) amino acid sequence analysis of the product S-bgl3 of beta-glucosidase gene S-bgl3 coding
One of beta-glucosidase gene S-bgl3 coding contains 438 amino acid whose protein, by the theoretical molecular size of this protein of DNAStar software prediction, is 47998.38 dalton.
With simple assemblies structural research instrument (Simple Modular Architecture Research Tool, SMART, http://smart.embl-heidelberg.de) analyze the unit construction of beta-glucosidase S-bgl3, result is that the 11-438 amino acids of holding from N is family's 1 glycosyl hydrolase (glycosyl hydrolase) functional domain.
5) clone of beta-glucosidase gene S-bgl3 and expression
Use upstream primer 5 '-ATC
cCATGGtG
cACCACCACCACCACCACcCTCGGACAGCTCAGACGGCTC-3 ' and downstream primer 5 '-CGT
aAGCTTtCACCGCTGGGCCCGCAGCACC-3 ', by polymerase chain reaction (PCR) amplification beta-glucosidase gene S-bgl3, with restriction enzyme Nco I and Hind III enzyme, cut after beta-glucosidase gene S-bgl3, be connected with the expression vector pSE380 cutting with Hind III enzyme through Nco I.To connect product CaCl again
2method is transformed in E.colistrain XL1 blue, is applied to containing on the LA flat board of 100 μ g/mL penbritins.Single bacterium colony point plate that conversion obtains sieves substratum again to the LA that contains 100 μ g/mL penbritins, Vitamin C2, ferric ammonium citrate, and (every 100mL sieves again substratum and contains: Vitamin C2: 0.2g, ferric ammonium citrate: 0.5g, peptone: 1g, yeast powder: 0.5g, NaCl:0.5g, agar powder: 1.5g) on flat board, flat board is placed in to 37 ℃ of thermostat containers to be cultivated 6 hours, each turning point bacterium colony is dripped to 1 μ L one by one and with LB substratum, be diluted to 1% IPTG abduction delivering, then, flat board being placed in to 37 ℃ of thermostat containers continues to cultivate 6 hours.After time arrives, carry out the stifling broken born of the same parents of chloroform, then flat board is placed in to 37 ℃ of thermostat containers the beta-glucosidase S-bgl3 expressing is reacted 8 hours with Vitamin C2 and ferric ammonium citrate.Observe multiple sieve dull and stereotyped.
Then be further extracted in the dull and stereotyped plasmid DNA that can form the clone of black circle of multiple sieve, and by its called after pSE-S-bgl3, with after restriction enzyme NcoI and HindIII complete degestion pSE-S-bgl3, carry out 0.8% agarose gel electrophoresis analysis, outside the DNA fragmentation of result pSE-S-bgl3 apart from an about 4.1kb, also have a size to be about the DNA fragmentation of 1.32kb.
The recombination bacillus coli XL1-Blue inoculation that contains plasmid pSE-S-bgl3 is contained in the LB substratum of 100 μ g/mL penbritins to 600mL, and 37 ℃ of shaking culture, treat OD
600be 0.4 o'clock, adding IPTG to make its final concentration is 0.5mmol/L, and 30 ℃, 200 turn induction 10 hours.The centrifugal 3min of 11000rpm, collects thalline, with the resuspended thalline of phosphoric acid buffer of 4mL pH7.0100mmol/L, and the broken born of the same parents of ultrasonic wave 9 minutes.The centrifugal 20min of 12000rpm, gets supernatant and carries out protein purification below.The nickel affinity chromatography colloid that adds 1mL50% by every 4mL supernatant liquor, turns and shakes 60 minutes with 200 at 4 ℃, and mixture is filled into pillar, collects effluent.Add 1mL dcq buffer liquid (50mmol/L NaH
2pO
4, 300mmol/L NaCl, 20mmol/L imidazoles, pH8.0) in pillar, slowly stirs, and collects effluent.Repeat rinse step 4 times.Add elution buffer (50mmol/L NaH
2pO
4, 300mmol/L NaCl, 250mmol/L imidazoles, pH8.0) elute protein.Collect the protein soln of wash-out, with polyacrylamide gel electrophoresis (SDS-PAGE) checking of sex change, find that there is the protein band of object size.
6) mensuration of beta-glucosidase S-bgl3 hydrolysis fiber disaccharides
Beta-glucosidase S-bgl3 be take cellobiose as substrate, act on 30 minutes at the Lin acid hydrogen Er Na – phosphate sodium dihydrogen buffer solution of pH7.0 and 37 ℃.After reaction times arrives, in 10 minutes termination reactions of 100 ℃ of heating.The high performance liquid chromatography for product (HPLC) of S-bgl3 hydrolysis fiber disaccharides detects.
The result that HPLC detects shows: S-bgl3 can generate glucose by hydrolysis fiber disaccharides.
HPLC condition: instrument: Agilent1100 chromatographic instrument; Chromatographic column: nh 2 column; Moving phase: acetonitrile: water (70:30); Flow velocity: 1.0mL/min; Detector: RID(refraction detector).
It is 146.12U/mg that S-bgl3 be take the enzyme activity that cellobiose is substrate.
Claims (4)
1. a beta-glucosidase gene
s-bgl3, it is characterized in that, its nucleotide sequence is as shown in SEQ ID NO:1.
2. according to the protein of the coded by said gene of claim 1, its aminoacid sequence is as shown in SEQ ID NO:2.
3. an expression vector, is characterized in that, it contains gene claimed in claim 1.
4. a host cell, is characterized in that, it is prokaryotic cell prokaryocyte or the eukaryotic cell that expression vector transforms described in claim 3.
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| CN103266096A (en) * | 2013-06-07 | 2013-08-28 | 广西科学院 | Mutant Pbgl-W386C of beta-glucosidase and application thereof |
| CN105754973B (en) * | 2016-05-05 | 2019-01-29 | 广西大学 | A kind of mutant W233D of beta-glucosidase and its application |
| CN105969751B (en) * | 2016-06-14 | 2019-12-31 | 南京工业大学 | Beta-glucosidase gene and application thereof |
| CN108410924B (en) * | 2018-03-22 | 2021-10-26 | 广西大学 | Application of beta-xylosidase belonging to glycosyl hydrolase family 3 in hydrolysis of corncob xylan |
| CN113388628A (en) * | 2021-06-01 | 2021-09-14 | 浙江工业大学 | Beta-glucosidase gene, coding enzyme, engineering bacterium and application thereof |
Citations (2)
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|---|---|---|---|---|
| JPS6328389A (en) * | 1986-07-22 | 1988-02-06 | Res Assoc Petroleum Alternat Dev<Rapad> | Beta-glucosidase and production thereof |
| CN102477417A (en) * | 2010-11-24 | 2012-05-30 | 中国农业大学 | Beta-glucosaccharase and encoding gene and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6328389A (en) * | 1986-07-22 | 1988-02-06 | Res Assoc Petroleum Alternat Dev<Rapad> | Beta-glucosidase and production thereof |
| CN102477417A (en) * | 2010-11-24 | 2012-05-30 | 中国农业大学 | Beta-glucosaccharase and encoding gene and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| Antonio Rojas等.Frameshift mutation events in h-glucosidases.《Gene》.2003,第314卷191–199. |
| Bentley.Streptomyces coelicolor A3(2) complete genome.《genbank登录号AL939113.1》.2008, |
| Frameshift mutation events in h-glucosidases;Antonio Rojas等;《Gene》;20031231;第314卷;191-199 * |
| Streptomyces coelicolor A3(2) complete genome;Bentley;《genbank登录号AL939113.1》;20081023;见gene"SCO2531"的序列及信息 * |
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