CN103113391A - Method and equipment for decoloring and purifying 7-aminocephalosporanic acid based on ultra-filtration membrane technique - Google Patents
Method and equipment for decoloring and purifying 7-aminocephalosporanic acid based on ultra-filtration membrane technique Download PDFInfo
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- CN103113391A CN103113391A CN2012105692989A CN201210569298A CN103113391A CN 103113391 A CN103113391 A CN 103113391A CN 2012105692989 A CN2012105692989 A CN 2012105692989A CN 201210569298 A CN201210569298 A CN 201210569298A CN 103113391 A CN103113391 A CN 103113391A
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Abstract
The invention discloses a method and equipment for decoloring and purifying 7-aminocephalosporanic acid based on an ultra-filtration membrane technique. The method comprises the following steps of: decoloring and purifying the 7-aminocephalosporanic acid generated from enzymic-method splitting decomposition by using an ultra-filtration membrane separating system, and supplying deionized water, pure water or nanofiltration dialysis water in the process to carry out product filter wash so as to decolor and purify the 7-aminocephalosporanic acid. According to the method and the equipment, the ultra-filtration membrane separating system takes the place of the conventional activated carbon decoloration process to decolor and purify the 7-aminocephalosporanic acid; and compared with the activated carbon decoloration process, the method and the equipment need no activated carbon, can effectively improve the product quality and lower the production cost.
Description
Technical field
The present invention relates to the technical field of pharmacy, espespecially a kind of 7-amino-cephalosporanic acid decolorization and purification method and apparatus based on ultrafiltration membrane technique.
Background technology
7-amino-cephalosporanic acid (being called for short 7-ACA) is the antibiotic critical materials of semi-synthetic cephalosporins of production clinical application, and main cephalosporin by fermentative production (being called for short CPC) produces 7-ACA by enzymatic cleavage at present.Owing to still can producing the impurity such as a large amount of albumen and pigment in the enzymatic cleavage process, larger impact yield and the quality of product.So before crystallization, after still needing to use gac to carry out the impurity such as adhesion protein and pigment, then by the filtering activated carbon granule, to reach the effect of decolorization and purification.But the main problem of activated carbon decolorizing purification is:
1. annual gac usage quantity is large, and use cost is high;
2. the waste residue that produces not only makes Working environment comparatively abominable, and operator's is healthy also influenced;
3. product yield is lower;
4. the quality product fluctuation range is larger.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of 7-amino-cephalosporanic acid decolorization and purification method and apparatus based on ultrafiltration membrane technique, and it replaces gac to decolour with ultra-filtration membrane, to improve the quality of products and yield.
For solving the problems of the technologies described above, technical solution of the present invention is:
A kind of 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique, the 7-amino-cephalosporanic acid that will produce through enzymatic cleavage, carry out decolorization and purification by the ultra-filtration membrane separation system, and replenish deionized water, pure water or nanofiltration water-dialyzing and carry out product and wash rate in this process, thereby 7-amino-cephalosporanic acid is carried out decolorization and purification.
A kind of 7-amino-cephalosporanic acid decolorization and purification equipment based on ultrafiltration membrane technique comprises cartridge filter, ultrafiltration membrane system and for delivery of the filter wash device of deionized water, pure water or nanofiltration water-dialyzing; The outlet of this cartridge filter is connected with the entrance of ultra-filtration membrane separation system, and the entrance of this ultra-filtration membrane separation system is connected with the filter wash device simultaneously.
The concentrated solution outlet of described ultra-filtration membrane separation system directly discharges by pipeline on the one hand, turns back to the entrance of ultra-filtration membrane separation system by pipeline on the one hand, and be respectively equipped with valve on discharge tube and Returning pipe.
Utilize a kind of 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique of aforesaid device, comprise the steps:
(1) coarse filtration: the 7-amino-cephalosporanic acid that produces through enzymatic cleavage is introduced into cartridge filter and removes solid substance impurity, carries out coarse filtration;
(2) ultra-filtration membrane separation system decolorization and purification:
The enzymolysis 7-ACA solution of process coarse filtration enters the ultra-filtration membrane separation system and carries out decolorization and purification, and this ultra-filtration membrane separation system gained filtrate is the 7-amino-cephalosporanic acid after purification.
(3) filter wash:
In ultra-filtration membrane separation system operation, add deionized water, pure water or nanofiltration water-dialyzing by the filter wash device to the entrance of ultra-filtration membrane separation system, make the 7-ACA solution of concentrated solution one side fully see through ultra-filtration membrane and improve yield.
Comprise that further the circulation of step (4) ultra-filtration membrane separation system concentrated solution is concentrated: in described step (3), ultra-filtration membrane separation system gained concentrated solution turns back to system entry in the filter wash process, when the 7-ACA product of concentrated solution one side is eluted to the residual value of allowing, close the valve on Returning pipe, the valve on the unlatching discharge tube, concentrated solution is discharged system as dense slag.
After adopting such scheme, because utilizing film, the present invention holds back the characteristic of solute, replace existing activated carbon decolorizing technique with the ultra-filtration membrane separation system, 7-amino-cephalosporanic acid is carried out decolorization and purification, with respect to activated carbon decolorizing technique, need not use gac, can effectively improve the quality of products, and can reduce production costs.
In addition, the present invention also has following advantage:
1. owing to not using gac, therefore can not produce waste residue, can cleaning, safety, High-efficient Production.
2. can carry out the product filter wash by replenishing deionized water etc., make product see through fully membrane module, improve product yield.
Description of drawings
Fig. 1 is the structural representation of equipment of the present invention.
Embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments.
Disclosed is a kind of 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique, the 7-amino-cephalosporanic acid (7-ACA) that will produce through enzymatic cleavage, carry out decolorization and purification by the ultra-filtration membrane separation system, and replenish deionized water, pure water or nanofiltration water-dialyzing and carry out product and wash rate in this process, finally obtain highly purified 7-amino-cephalosporanic acid.
What the present invention also disclosed is a kind of 7-amino-cephalosporanic acid decolorization and purification equipment based on ultrafiltration membrane technique, and as shown in Figure 1, this equipment comprises cartridge filter 1, ultrafiltration membrane system 2 and filter wash device 3.The outlet of this cartridge filter 1 is connected with the entrance of ultra-filtration membrane separation system 2, and the entrance of this ultra-filtration membrane separation system 2 is connected with filter wash device 3 simultaneously.This filter wash device 3 is for carrying the device of deionized water, pure water or nanofiltration water-dialyzing.
Further, the concentrated solution outlet of described ultra-filtration membrane separation system 2 directly discharges by pipeline on the one hand, turns back to the entrance of ultra-filtration membrane separation system 2 by pipeline on the one hand, and be respectively equipped with valve 6,5 on discharge tube 7 and Returning pipe 4.
Utilize aforesaid device, concrete technology step of the present invention is:
(1) coarse filtration:
Owing to containing the impurity such as fragment of a large amount of pigments, protein-colloid and enzyme in enzymolysis 7-ACA solution, this solution is introduced into the solid substance impurity such as fragment that cartridge filter 1 is removed enzyme, carries out coarse filtration.
(2) ultra-filtration membrane separation system decolorization and purification:
The enzymolysis 7-ACA solution of process coarse filtration enters ultra-filtration membrane separation system 2 and carries out decolorization and purification, and these ultra-filtration membrane separation system 2 gained filtrates are highly purified 7-amino-cephalosporanic acid.
(3) filter wash:
In ultra-filtration membrane separation system operation, add deionized water, pure water or nanofiltration water-dialyzing by filter wash device 3 to the entrance of ultra-filtration membrane separation system 2, make the 7-ACA solution of concentrated solution one side fully see through ultra-filtration membrane, to improve yield.
(4) circulation of ultra-filtration membrane separation system concentrated solution is concentrated:
In described step (3), ultra-filtration membrane separation system 2 gained concentrated solutions turn back to system entry in the filter wash process, and when the 7-ACA product of concentrated solution one side was eluted to the residual value of allowing, valve-off 5, Open valve 6 were discharged system with concentrated solution as dense slag.
The above is only preferred embodiment of the present invention, is not to limit scope of the invention process.Therefore variation or the modification in every case done according to claim of the present invention and specification sheets are within all should belonging to the scope that patent of the present invention contains.
Claims (5)
1. 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique, it is characterized in that: the 7-amino-cephalosporanic acid that will produce through enzymatic cleavage, carry out decolorization and purification by the ultra-filtration membrane separation system, and replenish deionized water, pure water or nanofiltration water-dialyzing and carry out product and wash rate in this process, thereby 7-amino-cephalosporanic acid is carried out decolorization and purification.
2. 7-amino-cephalosporanic acid decolorization and purification equipment based on ultrafiltration membrane technique is characterized in that: comprise cartridge filter, ultrafiltration membrane system and for delivery of the filter wash device of deionized water, pure water or nanofiltration water-dialyzing; The outlet of this cartridge filter is connected with the entrance of ultra-filtration membrane separation system, and the entrance of this ultra-filtration membrane separation system is connected with the filter wash device simultaneously.
3. the 7-amino-cephalosporanic acid decolorization and purification equipment based on ultrafiltration membrane technique according to claim 2, it is characterized in that: the concentrated solution outlet of described ultra-filtration membrane separation system directly discharges by pipeline on the one hand, turn back to the entrance of ultra-filtration membrane separation system by pipeline on the one hand, and be respectively equipped with valve on discharge tube and Returning pipe.
4. utilize a kind of 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique as equipment as described in claim 2,3, it is characterized in that comprising the steps:
(1) coarse filtration: the 7-amino-cephalosporanic acid that produces through enzymatic cleavage is introduced into cartridge filter and removes solid substance impurity, carries out coarse filtration;
(2) ultra-filtration membrane separation system decolorization and purification:
The enzymolysis 7-ACA solution of process coarse filtration enters the ultra-filtration membrane separation system and carries out decolorization and purification, and this ultra-filtration membrane separation system gained filtrate is the 7-amino-cephalosporanic acid after purification;
(3) filter wash:
In ultra-filtration membrane separation system operation, add deionized water, pure water or nanofiltration water-dialyzing by the filter wash device to the entrance of ultra-filtration membrane separation system, make the 7-ACA solution of concentrated solution one side fully see through ultra-filtration membrane and improve yield.
5. the 7-amino-cephalosporanic acid decolorization and purification method based on ultrafiltration membrane technique according to claim 4, it is characterized in that further comprising that the circulation of step (4) ultra-filtration membrane separation system concentrated solution is concentrated: in described step (3), ultra-filtration membrane separation system gained concentrated solution turns back to system entry in the filter wash process, when the 7-ACA product of concentrated solution one side is eluted to the residual value of allowing, close the valve on Returning pipe, the valve on the unlatching discharge tube, concentrated solution is discharged system as dense slag.
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| CN2012105692989A CN103113391A (en) | 2012-12-25 | 2012-12-25 | Method and equipment for decoloring and purifying 7-aminocephalosporanic acid based on ultra-filtration membrane technique |
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| CN2012105692989A CN103113391A (en) | 2012-12-25 | 2012-12-25 | Method and equipment for decoloring and purifying 7-aminocephalosporanic acid based on ultra-filtration membrane technique |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104610283A (en) * | 2015-01-30 | 2015-05-13 | 悦康药业集团有限公司 | Cefepime dihydrochloride compound and pharmaceutical composition thereof |
| CN114230622A (en) * | 2021-11-12 | 2022-03-25 | 陕西麦可罗生物科技有限公司 | Method for decoloring kasugamycin concentrated solution |
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| CN102676616A (en) * | 2012-05-16 | 2012-09-19 | 成都连接流体分离科技有限公司 | Efficient and environment-friendly malt syrup producing process |
| CN203095921U (en) * | 2012-12-25 | 2013-07-31 | 厦门市天泉鑫膜科技股份有限公司 | 7-aminocephalosporanic acid decolorization and purification device based on ultra-filtration membrane technology |
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- 2012-12-25 CN CN2012105692989A patent/CN103113391A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102676616A (en) * | 2012-05-16 | 2012-09-19 | 成都连接流体分离科技有限公司 | Efficient and environment-friendly malt syrup producing process |
| CN203095921U (en) * | 2012-12-25 | 2013-07-31 | 厦门市天泉鑫膜科技股份有限公司 | 7-aminocephalosporanic acid decolorization and purification device based on ultra-filtration membrane technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104610283A (en) * | 2015-01-30 | 2015-05-13 | 悦康药业集团有限公司 | Cefepime dihydrochloride compound and pharmaceutical composition thereof |
| CN104610283B (en) * | 2015-01-30 | 2019-01-11 | 悦康药业集团有限公司 | A kind of cefepime hydrochloride compound and its pharmaceutical composition |
| CN114230622A (en) * | 2021-11-12 | 2022-03-25 | 陕西麦可罗生物科技有限公司 | Method for decoloring kasugamycin concentrated solution |
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Application publication date: 20130522 |