CN103105328A - Concentration technology for alpine grassland soil seed bank sample - Google Patents
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- CN103105328A CN103105328A CN2013100342004A CN201310034200A CN103105328A CN 103105328 A CN103105328 A CN 103105328A CN 2013100342004 A CN2013100342004 A CN 2013100342004A CN 201310034200 A CN201310034200 A CN 201310034200A CN 103105328 A CN103105328 A CN 103105328A
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Abstract
The invention discloses a concentration technology for an alpine grassland soil seed bank sample. The concentration technology comprises the following steps of: (1) cracking a soil sample by a rubber hammer as much as possible; (2) sieving impurities, such as stones, branches and leaves, by a soil sieve with the aperture of 6mm; (3) sieving relatively large stones and impurities by a soil sieve with the aperture of 2mm; (4) sieving fine earth by a soil sieve with the aperture of 0.1mm; (5) putting a dried soil sample into a nylon mesh bag; (6) washing the nylon mesh bag on a suspension frame made of reinforcing steel bars by water; and (7) drying the washed sample in a cloth bag to obtain the minimum sample to be analyzed. The concentration technology disclosed by the invention is specific for an alpine region, and has the advantages that the sample size can be greatly reduced, dust pollution can be alleviated, the accuracy of an analysis result can be improved, the labor efficiency can be improved, the loss and damage of seeds during soil seed bank researches can be avoided, and the germination rate of the seeds in a soil seed bank can be observably improved.
Description
Technical field
The present invention relates to a kind of concentration technique of soil seed bank, soil seed pool sample, particularly, relate to a kind of concentration technique of Alpine Grasslands soil seed bank, soil seed pool sample.
Background technology
Soil seed bank, soil seed pool research is the Hot Contents of global vegetation ecology research, but technical requirement is different in concrete research, and along with the variation of research object, method is different, but no matter which type of research method, the concentration technique of its pedotheque is that to be related to result accurate, and simplifies the key of work workload.Therefore, global researchist is more the development of the concentration technique of soil seed bank, soil seed pool sample, and the aspects such as equipment development of mechanical automation are even arranged.
No matter exploitation and the use of what concentration technique, primary requirement are can not damage seed and lose seed in concentration process.But different research areas are different because floristics consists of, and can not be applied to the another one area to other regional technology, and reason has three, 1) minimum seed particle size size is had any different, and causes the minimum-value aperture soil sieve to be chosen variant; Be 2) the method close relation of analytical technology and follow-up use, Microscopical Method For Detection? or sprouting method? this is relevant with different regions soil seed bank, soil seed pool richness; 3) consist of relevant with the different regions soil texture.Therefore, particular ecosystem, different vegetation should have the soil seed bank, soil seed pool sample concentration method of the uniqueness of oneself.
Alpine Grasslands occupies 51% of the Qinghai-Tibet total area (2,500,000 square kilometres), soil seed bank, soil seed pool research to deep understand the Alpine Grasslands vegetation ecology and effectively management have important value.In Qinghai-Tibet Alpine Grasslands research field, a large amount of soil seed bank, soil seed pool researchs are carried out at present, but effectively the pedotheque concentration technique does not also have detailed growing up.And a large amount of pedotheques are acquired under study for action, occupy large quantity space, and have caused a large amount of dust to pollute.Therefore effectively concentration technique is to save the labour, saves the space, reduces necessary that dust pollutes.Given this purpose, we have developed a feasible pedotheque concentration technique of cover for Alpine Grasslands soil seed bank, soil seed pool research, can guarantee not lose seed, not destroy seed again, more can reduce a large amount of labours, can also have to the accuracy of result of study significant facilitation.This has important using value carrying out on a large scale extremely frigid zones soil seed bank, soil seed pool research.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defective, provide a kind of and be specifically designed to extremely frigid zones, can reduce in a large number sample accommodating, reduced dust and polluted, improve the analysis result degree of accuracy, raise labour efficiency, avoid working out the active seed at soil seed bank, soil seed pool, damage seed, significantly improve soil seed bank, soil seed pool in the concentration technique of Alpine Grasslands soil seed bank, soil seed pool sample of seed germination rate.
In order to solve the problems of the technologies described above, the invention provides following technical scheme:
A kind of concentration technique of Alpine Grasslands soil seed bank, soil seed pool sample comprises the steps:
(1) break pedotheque into pieces with rubber hammer as far as possible;
(2) remove the foreign material such as stone, branches and leaves with 6 millimeters aperture soil sieve sieves;
(3) the soil sieve sieve with 2 millimeters apertures removes relatively large stone and foreign material;
(4) screen out fine earth with the soil sieve in 0.1 millimeter, aperture again;
(5) will shine rear pedotheque and put into nylon net bag;
(6) nylon net bag is put on the hanger that reinforcing bar does, water rinses;
(7) wash rear sample and be put in cloth bag and dry, obtain the stand-by sample of analysis of volume minimum.
Particularly, totally 10 of described nylon net bags, specification is: 30cm * 30cm, 0.1 millimeter, aperture; One of the hanger that reinforcing bar is done, specification is: high 1 meter, long 2 meters; The specification of cloth bag is: long 20cm, wide 15cm.
The present invention has following beneficial effect:
Alpine Grasslands soil seed bank, soil seed pool Sample Preconcentration Technique of the present invention is compared with existing technical method, has following features, and (1) is applicable to the thick characteristics of Alpine Grasslands sod layer, adopts the maximum diameter of hole soil sieve can be easy to screen more foreign material.(2) secondary eliminating impurities method greatly reduces the impact of the foreign material such as stone in extremely frigid zones soil.(3) sprout experimental result according to residue, determine the use of minimum-value aperture soil sieve, guarantee not occur seed and lose.(4) adopt in conjunction with the method for washing, guarantee large department fine earth in sample is washed off, guaranteed the output of dust amount in the operating process of microscopy method.(5) method of washing has played the effect of pre-breaking dormancy, but this makes the effect of the higher germination seed recovery of sprouting method research extremely frigid zones soil seed acquisition, because the extremely frigid zones seed germination rate is relatively low.
Description of drawings
Accompanying drawing is used to provide a further understanding of the present invention, and consists of the part of instructions, is used for together with embodiments of the present invention explaining the present invention, is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the hanger schematic diagram that reinforcing bar of the present invention is done;
Fig. 2 is soil seed bank, soil seed pool seed germination amount (/ square metre) comparison diagram of method of the present invention and contrast method;
Fig. 3 is the contained species number of the soil seed bank, soil seed pool seed of method of the present invention and contrast method (species number/square metre) comparison diagram.
Embodiment
The concentration technique of Alpine Grasslands soil seed bank, soil seed pool sample of the present invention comprises the steps:
(1) break pedotheque into pieces with rubber hammer as far as possible;
(2) remove the foreign material such as stone, branches and leaves with 6 millimeters aperture soil sieve sieves;
(3) the soil sieve sieve with 2 millimeters apertures removes relatively large stone and foreign material;
(4) screen out fine earth with the soil sieve in 0.1 millimeter, aperture again;
(5) will shine rear pedotheque and put into nylon net bag;
(6) nylon net bag 2 is put on the hanger 1 that reinforcing bar does, water rinses, specifically as shown in Figure 1;
(7) wash rear sample and be put in cloth bag and dry, obtain the stand-by sample of analysis of volume minimum.
In above step, totally 10 of described nylon net bags, specification is: 30cm * 30cm, 0.1 millimeter, aperture; One of the hanger that reinforcing bar is done, specification is: high 1 meter, long 2 meters; The specification of cloth bag is: long 20cm, wide 15cm.
With the prior art Piglet s colibacillosis:
As shown in Figures 2 and 3, compare with not adopting this method, this method has improved the seed amount of sprouting in seed bank in the pedotheque of analyzing, and between 11-82%, has improved simultaneously acquisition species number between 30-100%.This method is little at Alpine Grasslands soil seed bank, soil seed pool seed particle size, and germination rate is low, under the characteristics of seed dormancy phase length, can improve the degree of accuracy of soil seed bank, soil seed pool research.Sample 1-5 from the pedotheque in the high carelessly pasture of Alpine Grasslands, is the soil seed bank, soil seed pool sample that the meadow takes from the ground 10cm degree of depth respectively, and five samples have adopted respectively this method to carry out sprouting after concentration again, and not concentrated two kinds of methods of direct germination.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment, the present invention is had been described in detail, for a person skilled in the art, it still can be modified to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (2)
1. the concentration technique of an Alpine Grasslands soil seed bank, soil seed pool sample, is characterized in that, comprises the steps:
(1) break pedotheque into pieces with rubber hammer as far as possible;
(2) remove the foreign material such as stone, branches and leaves with 6 millimeters aperture soil sieve sieves;
(3) the soil sieve sieve with 2 millimeters apertures removes relatively large stone and foreign material;
(4) screen out fine earth with the soil sieve in 0.1 millimeter, aperture again;
(5) will shine rear pedotheque and put into nylon net bag;
(6) nylon net bag is put on the hanger that reinforcing bar does, water rinses;
(7) wash rear sample and be put in cloth bag and dry, obtain the stand-by sample of analysis of volume minimum.
2. the concentration technique of Alpine Grasslands soil seed bank, soil seed pool sample according to claim 1, is characterized in that, totally 10 of described nylon net bags, and specification is: 30cm * 30cm, 0.1 millimeter, aperture; One of the hanger that reinforcing bar is done, specification is: high 1 meter, long 2 meters; The specification of cloth bag is: long 20cm, wide 15cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278372A (en) * | 2013-05-17 | 2013-09-04 | 中国科学院东北地理与农业生态研究所 | Method for rapidly identifying variety and quantity of species of moderately severe soda salinized grassland soil seed bank |
| CN106305038A (en) * | 2016-07-28 | 2017-01-11 | 长白山科学研究院 | Net bag type vegetation ecological remediation method based on soil seed bank technology |
| CN110018297A (en) * | 2019-05-29 | 2019-07-16 | 中国科学院植物研究所 | A kind of soil seed bank, soil seed pool identification method and Double layer culture disk |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009062758A (en) * | 2007-09-07 | 2009-03-26 | Mito Green Service:Kk | Natural vegetation inducing net, and natural vegetation inducing structure for slope |
| CN102094067A (en) * | 2010-12-10 | 2011-06-15 | 东北林业大学 | Microbiology prediction method for seasonal wet meadow soil nutrient of high-latitude natural wetland |
| CN201983953U (en) * | 2011-01-12 | 2011-09-21 | 中国科学院新疆生态与地理研究所 | Sampling device for wandering dune soil seed bank |
-
2013
- 2013-01-29 CN CN2013100342004A patent/CN103105328A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009062758A (en) * | 2007-09-07 | 2009-03-26 | Mito Green Service:Kk | Natural vegetation inducing net, and natural vegetation inducing structure for slope |
| CN102094067A (en) * | 2010-12-10 | 2011-06-15 | 东北林业大学 | Microbiology prediction method for seasonal wet meadow soil nutrient of high-latitude natural wetland |
| CN201983953U (en) * | 2011-01-12 | 2011-09-21 | 中国科学院新疆生态与地理研究所 | Sampling device for wandering dune soil seed bank |
Non-Patent Citations (6)
| Title |
|---|
| MIAOJUN MA ET AL.: "Soil seed bank dynamics in alpine wetland succession on the Titetan Plateau", 《PLANT AND SOIL》 * |
| YUAN-YUAN LI ET AL.: "Soil seed banks in degraded and revegetated grasslands in the aipine region of the Qinghai-Tibetan Plateau", 《ECOLOGICAL ENGINEERING》 * |
| 尚占环 等: "青藏高原"黑土型"退化高寒草地可萌发土壤种子库数量特征及种子萌发动态", 《草业科学增刊》 * |
| 尚占环 等: "黄河源区退化高寒草地土壤种子库：种子萌发的数量和动态", 《应用与环境生物学报》 * |
| 张黎敏 等: "土壤筛对一定深度不同退化高寒草甸土壤种子库的分选效果", 《草原与草坪》 * |
| 邓斌 等: "封育三年对三种高寒草地群落土壤种子库的影响", 《草业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278372A (en) * | 2013-05-17 | 2013-09-04 | 中国科学院东北地理与农业生态研究所 | Method for rapidly identifying variety and quantity of species of moderately severe soda salinized grassland soil seed bank |
| CN103278372B (en) * | 2013-05-17 | 2015-07-15 | 中国科学院东北地理与农业生态研究所 | Method for rapidly identifying variety and quantity of species of moderately severe soda salinized grassland soil seed bank |
| CN106305038A (en) * | 2016-07-28 | 2017-01-11 | 长白山科学研究院 | Net bag type vegetation ecological remediation method based on soil seed bank technology |
| CN106305038B (en) * | 2016-07-28 | 2019-07-16 | 长白山科学研究院 | Net-bag type vegetation ecological restorative procedure based on soil seed bank, soil seed pool technology |
| CN110018297A (en) * | 2019-05-29 | 2019-07-16 | 中国科学院植物研究所 | A kind of soil seed bank, soil seed pool identification method and Double layer culture disk |
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Application publication date: 20130515 |