CN103091198A - Soybean protein fiber content detection method - Google Patents

Abstract

The invention discloses a soybean protein fiber content detection method comprising the steps such as pretreatment, sodium hypochlorite solution treatment, residual insoluble fiber dry weight measurement, and the like. The method provided by the invention is simple and accurate, and provides good working effect.

Description

The detection method of soybean fiber content

Technical field

The present invention relates to a kind of detection method of fibre and soya content.

Background technology

For the detection of fibre and soya content, do not have very accurately and reliably and the convenient method of implementing in prior art, in the urgent need to further studying relevant detection method.

Summary of the invention

The object of the present invention is to provide that a kind of method is easy, the detection method of soybean fiber content accurately.

Technical solution of the present invention is:

A kind of detection method of soybean fiber content comprises the following steps:

(1) pre-service: get soybean sample 5g, be placed in soxhlet's extractor, use petroleum ether extraction 1h, per hour circulate at least six times; After the ether volatilization of sample PetroChina Company Limited., sample is immersed in cold water, to soak 1h, then soak 1h in 60 ℃ of-70 ℃ of water, the weight ratio of water and sample is 100: 1, stirring, is dried at then suction or centrifugal dehydration;

(2) get the pretreated sample that is no less than 1g and put into measuring cup, dry to constant weight at the baking ovens of 105 ℃ ± 3 ℃; Take out measuring cup, put into the cooling 30min of exsiccator, weigh, be accurate to 0.0002g; Then sample is put into tool plug Erlenmeyer flask, every gram sample adds the 100mL liquor natrii hypochloritis, shaking flasks is soaked sample, in 25 ℃ of insulation 30min, shake, with the glass core crucible filtration of known weight, remaining fiber is washed three times with the liquor natrii hypochloritis, last water cleans to neutral, after each washing first by the gravity discharge opeing, then with the discharge opeing of suction filtration device; Described liquor natrii hypochloritis's concentration is the 0.5mol/L sodium hypochlorite, and contains the NaOH that concentration is 20g/L;

The glass core crucible that (3) residual fiber will be housed is together with lid, and after putting into the baking oven of 105 ℃ ± 3 ℃ and drying to constant weight, cover lid moves into rapidly in exsiccator, and cooling 30min weighs, and is accurate to 0.0002g, obtains remaining the insoluble fiber dry weight; Calculate fibre and soya content according to formula (1):

$P=100d\×(1-\frac{r}{m})\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\left(1\right)$

In formula:

The clean dried percent content of P-soybean fiber, %;

D-correction factor gets 0.99;

R-residue insoluble fiber dry weight, g;

M-pretreated sample dry weight, g.

The inventive method is easy, accurate, good working effect.

The invention will be further described below in conjunction with embodiment.

Embodiment

A kind of detection method of soybean fiber content comprises the following steps:

(1) pre-service: get soybean sample 5g, be placed in soxhlet's extractor, use petroleum ether extraction 1h, per hour circulate at least six times; After the ether volatilization of sample PetroChina Company Limited., sample is immersed in cold water, to soak 1h, then soak 1h in 60 ℃ of-70 ℃ of water, the weight ratio of water and sample is 100: 1, stirring, is dried at then suction or centrifugal dehydration;

(2) get the pretreated sample that is no less than 1g and put into measuring cup, dry to constant weight at the baking ovens of 105 ℃ ± 3 ℃; Take out measuring cup, put into the cooling 30min of exsiccator, weigh, be accurate to 0.0002g; Then sample is put into tool plug Erlenmeyer flask, every gram sample adds the 100mL liquor natrii hypochloritis, shaking flasks is soaked sample, in 25 ℃ of insulation 30min, shake, with the glass core crucible filtration of known weight, remaining fiber is washed three times with the liquor natrii hypochloritis, last water cleans to neutral, after each washing first by the gravity discharge opeing, then with the discharge opeing of suction filtration device; Described liquor natrii hypochloritis's concentration is the 0.5mol/L sodium hypochlorite, and contains the NaOH that concentration is 20g/L;

The glass core crucible that (3) residual fiber will be housed is together with lid, and after putting into the baking oven of 105 ℃ ± 3 ℃ and drying to constant weight, cover lid moves into rapidly in exsiccator, and cooling 30min weighs, and is accurate to 0.0002g, obtains remaining the insoluble fiber dry weight; Calculate fibre and soya content according to formula (1):

$P=100d\×(1-\frac{r}{m})\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\left(1\right)$

In formula:

The clean dried percent content of P-soybean fiber, %;

D-correction factor gets 0.99;

R-residue insoluble fiber dry weight, g;

M-pretreated sample dry weight, g.

Claims (1)

1. the detection method of a soybean fiber content, is characterized in that: comprise the following steps:

(1) pre-service: get soybean sample 5g, be placed in soxhlet's extractor, use petroleum ether extraction 1h, per hour circulate at least six times; After the ether volatilization of sample PetroChina Company Limited., sample is immersed in cold water, to soak 1h, then soak 1h in 60 ℃ of-70 ℃ of water, the weight ratio of water and sample is 100:1, stirring, is dried at then suction or centrifugal dehydration;

(2) get the pretreated sample that is no less than 1g and put into measuring cup, dry to constant weight at the baking ovens of 105 ℃ ± 3 ℃; Take out measuring cup, put into the cooling 30min of exsiccator, weigh, be accurate to 0.0002g; Then sample is put into tool plug Erlenmeyer flask, every gram sample adds the 100mL liquor natrii hypochloritis, shaking flasks is soaked sample, in 25 ℃ of insulation 30min, shake, with the glass core crucible filtration of known weight, remaining fiber is washed three times with the liquor natrii hypochloritis, last water cleans to neutral, after each washing first by the gravity discharge opeing, then with the discharge opeing of suction filtration device; Described liquor natrii hypochloritis's concentration is the 0.5mol/L sodium hypochlorite, and contains the NaOH that concentration is 20g/L;

The glass core crucible that (3) residual fiber will be housed is together with lid, and after putting into the baking oven of 105 ℃ ± 3 ℃ and drying to constant weight, cover lid moves into rapidly in exsiccator, and cooling 30min weighs, and is accurate to 0.0002g, obtains remaining insoluble fiber; Calculate fibre and soya content according to formula (1):

$P=100d\×(1-\frac{r}{m})\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\·\left(1\right)$

In formula:

The clean dried percent content of P-soybean fiber, %;

D-correction factor gets 0.99;

R-residue insoluble fiber dry weight, g;

M-pretreated sample dry weight, g.