CN103083419A - Application of clematis root total saponins in preparing medicines inhibiting hyperuricemia and gout - Google Patents
Application of clematis root total saponins in preparing medicines inhibiting hyperuricemia and gout Download PDFInfo
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- CN103083419A CN103083419A CN 201110340153 CN201110340153A CN103083419A CN 103083419 A CN103083419 A CN 103083419A CN 201110340153 CN201110340153 CN 201110340153 CN 201110340153 A CN201110340153 A CN 201110340153A CN 103083419 A CN103083419 A CN 103083419A
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- total saponins
- gout
- radix clematidis
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Abstract
The invention discloses a novel purpose of clematis root total saponins, and specifically relates to an application of the clematis root total saponins in preparing medicines inhibiting hyperuricemia and gout. The clematis root total saponins are obtained with the method comprising the steps of: ethanol solution reflux extraction, extraction liquid concentration, centrifugation or filtration, solvent extraction of centrifugation liquid or filtrate, extraction liquid concentration, and macroporous resin enrichment. Clematis root total saponins content in prepared extract can be higher than 70%. As a result of pharmacological studies, the extract provided by the invention has a relatively high anti-inflammatory effect and an effect for reducing animal blood uric acid level. The clematis root total saponins can be used for preparing medicines used for treating hyperuricemia and gout.
Description
Technical field:
The invention belongs to pharmaceutical field, relate to the new purposes of Radix Clematidis total glycosides, be specifically related to the application of Radix Clematidis total glycosides in the anti-hyperuricemia of preparation and gout medicine.
Background technology:
Gout be by long-term purine metabolic disturbance and (or) caused one group of heterogeneous, the metabolic disease of underexcretion, sickness rate is very high.According to the data that Americanism damp disease association (ACR2010) annual meeting is announced, U.S.'s patient with gout has surpassed 8,000,000 people.In China, in the middle-aged male of Shanghai, the hyperuricemia sickness rate is 25% (Villegas R.Metabolic Syndrome and Related Disorders, 2010,8 (3): 263-270); In Shandong (Miao Z.Journal of Rheumatology, 2008,35 (9): 1859-1864), the sickness rate of hyperuricemia and gout is respectively 13.19% and 1.14%; And be ascendant trend year by year.Lack clinically specific medicament, medicine commonly used such as colchicine, allopurinol, benzbromarone etc. have serious liver, nephrotoxicity and bone marrow inhibition, are not suitable for life-time service.Seek anti-hyperuricemia and gout medicine efficient, low toxicity and become domestic and international drug research focus from plant.
Radix Clematidis is a kind of conventional Chinese medicine, reaches in ancient times modern hospital and is widely used clinically.The kind that China's pharmacopeia is recorded is ranunculaceae plant Radix Clematidis Clematis chinensis Osbeck, the dry root and rhizome of Clematis hexapetala Clematis hexapetala Pall. or northeast clematis Clematis manshurica Rupr..Contain the abundant chemical composition such as volatile oil, saponin, alkaloid, flavone, coumarin, lignanoid, have the pharmacological actions such as anti-inflammation, analgesia, relaxing smooth muscle, antitumor (Li Jia etc. the chemical composition of traditional Chinese medicine-Clematis chinensis and Advance on Pharmacological Activities [J], special product research, 2011 (1): 67-71).The Radix Clematidis saponins compound is the important active skull cap components of a class, have anti-inflammatory and antalgic (Xu Xianxiang etc. Radix Clematidis total glycosides anti-inflammatory and analgesic effect research, Pharmacology and Clinics of Chinese Materia Medica, 2005,21 (4): 34-35), the anti-immune inflammation (patent No.: the effect such as ZL200510040824.2); But have no the Radix Clematidis saponin aspect treatment hyperuricemia and gout biological activity and the report of clinical research.
Summary of the invention:
A kind of new purposes that the purpose of this invention is to provide Radix Clematidis total glycosides.
The new purposes of Radix Clematidis total glycosides provided by the present invention is the application of Radix Clematidis total glycosides in the anti-hyperuricemia of preparation and gout medicine; Described Radix Clematidis total glycosides is to prepare as follows: clematis chinensis medicinal materials adopts ethanol water to extract, after extracting solution is concentrated, and centrifugal or filtration; Filtrate or centrifugal liquid solvent extraction, macroporous resin enrichment after extract is concentrated prepares Radix Clematidis total glycosides.
Wherein, described clematis chinensis medicinal materials is the " kind that Chinese pharmacopoeia version in 2010 is recorded: cohosh Radix Clematidis Clematis chinensis Osbeck, the dry root and rhizome of Clematis hexapetala Clematis hexapetala Pall. or northeast clematis Clematis manshurica Rupr..
Described ethanol water extraction conditions is: extractant alcoholic solution concentration is 60%～95%, and solid-liquid ratio is 1: 8～1: 12, heating and refluxing extraction 45min～90min.
Described solvent extraction refers to that extracting solution is concentrated remove ethanol after, centrifugal or filter, filtrate or centrifugal liquid adopt ethyl acetate, n-butanol extraction after being diluted to concentration 0.1～0.2kg/L successively, collect the n-butanol extraction position, concentrating under reduced pressure obtains thick saponin.
Described macroporous resin enrichment, preferred AB-8 type, DA-201 type or D-101 macroporous resin.20%, 60%, 95% ethanol gradient elution is collected 60% eluting part, and drying under reduced pressure can obtain Radix Clematidis total glycosides after pulverizing and sieving.
Extractive of total saponin of clematis root provided by the invention prepares gained by above-mentioned method of purification, is mainly the pentacyclic triterpene oleanane glycoside; Total saponin content reaches more than 70%.Zoopery demonstration, this extract have stronger antiinflammatory and reduce the effect of animal blood uric acid level, can be prepared into the medicine for the treatment of hyperuricemia and gout.
The specific embodiment:
The following examples can make the present invention of those skilled in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1: the preparation of extract
Take clematis chinensis medicinal materials 10kg, add 60% concentration ethanol 120L, reflux, extract, 60min is after extracting solution is concentrated, centrifugal; Centrifugal liquid adopts ethyl acetate, n-butanol extraction after being diluted to concentration 0.2kg/L successively, collects the n-butanol extraction position, and concentrating under reduced pressure obtains thick saponin.Thick saponin with water dissolution after the AB-8 macroporous resin adsorption, 20%, 60%, 95% ethanol gradient elution is collected 60% eluting part, evaporated under reduced pressure gets the 225g extract, total saponin content 72.4%.
Embodiment 2: the preparation of extract
Take clematis chinensis medicinal materials 10kg, add 80% concentration ethanol 80L, reflux, extract, 90min is after extracting solution is concentrated, centrifugal; Centrifugal liquid adopts ethyl acetate, n-butanol extraction after being diluted to concentration 0.15kg/L successively, collects the n-butanol extraction position, and concentrating under reduced pressure obtains thick saponin.Thick saponin with water dissolution after the DA-201 macroporous resin adsorption, 20%, 60%, 95% ethanol gradient elution is collected 60% eluting part, evaporated under reduced pressure gets the 192g extract, total saponin content 80.8%.
Embodiment 3: the preparation of extract
Take clematis chinensis medicinal materials 10kg, add 95% concentration ethanol 100L, reflux, extract, 45min after extracting solution is concentrated, filters; Filtrate adopts ethyl acetate, n-butanol extraction after being diluted to concentration 0.1kg/L successively, collects the n-butanol extraction position, and concentrating under reduced pressure obtains thick saponin.Thick saponin with water dissolution after the D-101 macroporous resin adsorption, 20%, 60%, 95% ethanol gradient elution is collected 60% eluting part, evaporated under reduced pressure gets the 176g extract, total saponin content 78.3%.
Embodiment 4: pharmacodynamic study
Laboratory animal: the Kunming kind, cleaning level mice, 100, male, 15-20g is provided by Shanghai Slac Experimental Animal Co., Ltd., quality certification numbering: 2007000524284; First raise before experiment and adapted to for 1 week.
4.1 mice ear experimental technique and result
50 of male mices divide into groups at random, every group 10, i.e. blank group, three sample sets and positive drug aspirin group, blank group gives 0.4% tween 80, and sample sets gives corresponding test sample, it is 0.2g/kg that positive group gives aspirin, the gavage volume is the 15ml/kg body weight, gives continuously 7 days, 1h after the last administration, it is scorching that mouse right ear is evenly coated 25 μ l caused by dimethylbenzene xylene, puts to death animal after 15min.Cut left and right two ears, sweep away auricle with diameter 8mm card punch at same position, weigh, with left and right auricle weight difference value representation inflammatory swelling degree, between its ear swelling degree is organized, relatively, t checks.
Swelling (g)=auris dextra sheet weight-left auricle weight
Table 1 xylol cause mice ear impact (
N=10)
*: compare with model group
*P＜0.05,
*P＜0.01
4.2 hyperuricemia experimental technique and result
48 of male mices divide into groups at random, 8 every group.Be the blank group of normal saline group, hyperuricemia, three sample sets and positive drug allopurinol group.Adopt oxonic acid potassium salt method inducing mouse to produce hyperuricemia, front 1 hour in administration every day, except normal group, all the other organize respectively respectively that gavage gives Oteracil Potassium solution (250mgkg
-1), the administration volume is 15mL/kg, the sample dosage is as shown in the table.Experimental result employing SPSS11.5 statistical software processes, represent with MEAN ± S.E.M, group difference adopts one factor analysis of variance (F check), variance has homogeneous, more adopt in twos the LSD check, take P＜0.05 as the significant difference standard, P＜0.01 is the significant differences standard.
The impact of the mouse hyperuricemia that table 2 sample is induced oxonic acid potassium salt (
N=8)
+: compare with normal group
+P＜0.05,
++P＜0.01
*: compare with model group
*P＜0.05,
*P＜0.01
4.3 pharmacological evaluation is summed up
By results of animal as seen, Radix Clematidis total glycosides has significant antiinflammatory and reduces the effect of animal serum uric acid level, compares with model group to have significance and significant differences, can be used for preparing the medicine of anti-hyperuricemia and gout.
Claims (2)
1. the application of Radix Clematidis total glycosides in preparation treatment hyperuricemia and gout medicine is characterized in that Radix Clematidis total glycosides is to be prepared by following method:
1. extract: clematis chinensis medicinal materials adopts the alcoholic solution heating and refluxing extraction 45min～90min of 60%～95% concentration, and solid-liquid ratio is 1: 8～1: 12;
2. extraction: extracting solution concentrates, remove ethanol after, filter or centrifugal, filtrate or centrifugal liquid are diluted to 0.1～0.2kg/L; Adopt successively ethyl acetate, n-butanol extraction;
3. purification: after n-butanol portion was concentrated, macroporous resin separated; Preferred AB-8 type, DA-201 type or D-101 macroporous resin; 20%, 60%, 95% ethanol gradient elution is collected 60% eluting part, concentrating under reduced pressure;
4. dry: the concentrated solution vacuum drying, pulverized 100 mesh sieves, prepare Radix Clematidis total glycosides.
2. the application of Radix Clematidis total glycosides as claimed in claim 1 in preparation treatment hyperuricemia and gout medicine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208479A (en) * | 2014-09-12 | 2014-12-17 | 灌阳千家洞瑶之源生物科技有限公司 | Yao medicine composition for treating gout and preparation method thereof |
| CN115671123A (en) * | 2021-07-21 | 2023-02-03 | 苏州凯祥生物科技有限公司 | Application of clematis root saponin in preparation of purine-lowering medicine |
-
2011
- 2011-11-01 CN CN 201110340153 patent/CN103083419A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208479A (en) * | 2014-09-12 | 2014-12-17 | 灌阳千家洞瑶之源生物科技有限公司 | Yao medicine composition for treating gout and preparation method thereof |
| CN115671123A (en) * | 2021-07-21 | 2023-02-03 | 苏州凯祥生物科技有限公司 | Application of clematis root saponin in preparation of purine-lowering medicine |
| CN115671123B (en) * | 2021-07-21 | 2024-03-22 | 苏州凯祥生物科技有限公司 | Application of clematis root saponin in preparation of purine-reducing medicament |
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Application publication date: 20130508 |