CN103083379A - Preparation method and application of snow chrysanthemum flavonoids - Google Patents
Preparation method and application of snow chrysanthemum flavonoids Download PDFInfo
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- CN103083379A CN103083379A CN2013100646283A CN201310064628A CN103083379A CN 103083379 A CN103083379 A CN 103083379A CN 2013100646283 A CN2013100646283 A CN 2013100646283A CN 201310064628 A CN201310064628 A CN 201310064628A CN 103083379 A CN103083379 A CN 103083379A
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Abstract
The invention relates to a preparation method and an application of snow chrysanthemum flavonoids. A natural product snow chrysanthemum is extracted and separated by using a membrane separation technique combined with an aqueous two-phase extraction technology to obtain the snow chrysanthemum flavonoids. Tests confirm that the snow chrysanthemum flavonoids obtained by using the method have the effects of clearing oxygen radical, resisting oxidization, delaying senescence and whitening skin; and a new application value of the snow chrysanthemum is expanded.
Description
Technical field
The invention belongs to active ingredient of natural product extractive technique field, particularly a kind ofly extract the extracting method that separates total flavones from natural product snow chrysanthemum, also relate to simultaneously this total flavones as the application of antioxidant in medicine and cosmetic industry.
Background technology
The snow chrysanthemum is not independently species, is the trade name that is grown in the Herba Coreopsis tinctoria in Xinjiang in fact, and its formal name used at school is: Herba Coreopsis tinctoria, double-colored Coreopsis basalis.People are referred to as in Xinjiang: snow chrysanthemum, the Kunlun snow chrysanthemum, the Kunlun blood chrysanthemum, Tianshan Mountains snow chrysanthemum, high and cold snow chrysanthemum, high and cold Folium Crossostephii Chinensis etc.It is that the Uygur nationality passes on collection health preserving, health care and dimension medical material natural plants all over the body from generation to generation, can treat the diseases such as scorching excessive thirst, hypertension, nervous, gastrointestinal upset, inappetence and furuncle toxic swelling.The snow chrysanthemum due to growing environment than of low pollution, be rich in multiple to the human body beneficiating ingredient, therefore come as a kind of tea-drinking is universal gradually now.At present, isolation identification goes out more than 20 classes from the snow chrysanthemum, more than 300 kind of natural component, comprise more than 30 kind of Flavonoid substances, the mineral element of more than 30 kind of needed by human, 20 several amino acids, tens of kinds of aromatic compounds also have abundant organic acid, terpenes, vitamin, lignan, enzyme, polysaccharide etc. to have bioactive natural component.Wherein, general flavone content can reach 12% left and right, considerably beyond other various chrysanthemum classes.Modern pharmacological research shows, avenges the Flavonoid substances in chrysanthemum, is a kind of very strong antioxidant, can effectively remove the oxygen-derived free radicals in body.Free Radical Biology studies show that, oxygen-derived free radicals can cause the oxidative damage of biomolecule such as protein, lipid and DNA, can cause the multiple diseases such as cardiovascular and cerebrovascular disease, diabetes, cancer.And the ability of this prevention oxygen-derived free radicals of snow chrysanthemum total flavones oxidation is more than 10 times of vitamin E, can effectively stop the generation of degeneration, aging and the canceration of cell.Application in medicine and cosmetic industry has not yet to see report to this total flavones as antioxidant.Therefore, this total flavones having broad application prospects and researching value in medicine and cosmetic industry.
The extraction of relevant snow chrysanthemum total flavones separates currently reported, mainly contains 1. water extract-alcohol precipitation, resin separation purification; 2. organic solvent extraction, the macroporous resin separation and purification.These methods all exist extraction ratio lower to some extent, and consumption of organic solvent is large, the processing time is long, environmental pollution is serious, be unfavorable for the shortcomings such as health.Therefore study environmental protection, the Chinese medicine ingredients extraction separation method has become one of advanced subject of current biological medicine simply and easily.Membrane separation technique is a kind of use semipermeable membrane separation method, its separation principle is the size according to the material molecule yardstick, selection osmosis by film, under the impetus of outside energy or chemical potential difference to mixture in bi-component or multi-component Solute separates with solvent, processing and enrichment, thereby reach separation, purify and concentrated purpose.The double water phase separation technology refers between some Organic substance or between Organic substance and inorganic salt, with after suitable concentration dissolving, forming two-phase or multiphase immiscible system is double-aqueous phase system in water.Utilize different material that different partition coefficients is arranged in double-aqueous phase system, after material enters double-aqueous phase system, carry out selectivity and distribute between upper and lower phase, show certain partition coefficient, thereby reach the purpose of separation and purification.Membrane separation technique associating double water phase separation technology has great advantage to the extraction separation of natural product, by the different component in system is realized optionally separating, can reach separation and purification on molecular level.This technology belongs to physical separation method, has that loss of activity is little, separating step is few, operating condition is gentle, is easy to that technique is amplified and the advantage such as continued operation.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method and purposes of avenging the chrysanthemum total flavones, the method adopts membrane separation technique associating double water phase separation technology to carry out extracting separation to natural product snow chrysanthemum, obtain avenging the chrysanthemum total flavones, the snow chrysanthemum total flavones that obtains by the method confirms that through test snow chrysanthemum total flavones has the effect of removing oxygen-derived free radicals resisting oxidation and delaying senility and whitening, has opened up the new using value of snow chrysanthemum.
A kind of preparation method of avenging the chrysanthemum total flavones of the present invention follows these steps to carry out:
A kind of preparation method of avenging the chrysanthemum total flavones is characterized in that following these steps to carrying out:
A, the broken 20-60 mesh sieve of crossing of the snow inulin of drying is become medicated powder, the concentration of doubly measuring with 8-10 is alcohol heating reflux 1-2 hour of 60-80%, extracts 2-3 time, merge extractive liquid, filters, and reclaims ethanol, and be concentrated into when temperature 50 C that to measure relative density be 1.10-1.20, obtain concentrated solution;
B, with the 1:1 dilution by volume of step a concentrated solution water, standing, filter, filtrate is got centrifugal rear gained supernatant with 15000 rev/mins of centrifugalize, then filters except heavy with the micro-filtration membrane of aperture 0.02-0.1 μ m, transmembrane pressure is 0.5Mpa-1.0Mpa, feed temperature 25-35 ℃, obtains permeate;
C, the permeate of step b is carried out hollow fiber ultrafiltration membrane separate, the little 0.5Mpa-1.0Mpa of transmembrane pressure, feed temperature is 25-35 ℃, obtains ultrafiltrate;
D, the ultrafiltrate of step c is carried out hollow fiber nanofiltration membrane again separate, transmembrane pressure is 1.0Mpa-2.0Mpa, and feed temperature is 25-35 ℃, gets concentrated solution;
E, with steps d gained concentrated solution concentrating under reduced pressure, dry decorating film;
F, the decorating film of step e is adopted aqueous two-phase extraction, room temperature 2000-4000 rev/min centrifugal, it is biphase that double-aqueous phase system is divided into the up and down, gets the phase concentrating under reduced pressure, drying can obtain avenging the chrysanthemum total flavones.
The molecular cut off of step c hollow fiber ultrafiltration membrane is 2000-8000.
The molecular cut off of steps d hollow fiber nanofiltration membrane is 200-600.
The system of step f aqueous two-phase extraction is for pressing 3-5mL ethanol+5-7mL water+2-4g ammonium sulfate+0.15g decorating film mix homogeneously.
The purposes of the snow chrysanthemum total flavones that described method obtains in the medicine of preparation antioxidant.
The purposes of the snow chrysanthemum total flavones that described method obtains in preparation adornment product industry.
A kind of preparation method of avenging the chrysanthemum total flavones of the present invention, the snow chrysanthemum Determination Method of Flavone Content that obtains by the method:
Utilize the aluminum salt complex that generates flavone after flavone compound and aluminum chloride effect and the yellow depth becomes the principle of certain proportionate relationship with the content of flavone, carry out quantitative assay to avenging the chrysanthemum total flavones.
1, the preparation of need testing solution: precision takes 105 ℃ of temperature, and to be dried to the snow chrysanthemum total flavones of constant weight appropriate, adds dissolve with methanol, quantitatively transfers in the 10mL volumetric flask, adds methanol and be diluted to scale, shakes up, as need testing solution;
2, the preparation of reference substance solution: precision takes the appropriate 10.05mg of control substance of Rutin that 105 ℃ of temperature are dried to constant weight, adds methanol to make its dissolving, is transferred in the 25mL volumetric flask, and standardize solution shakes up and namely gets rutin titer (0.402mg/mL);
3, the preparation of standard curve: the accurate reference substance solution liquid 0.5,1.0,2.0,3.0,4.0 of drawing, 5.0mL is placed in respectively the 25mL volumetric flask, adds water to 6mL, adds respectively successively in order 5% NaNO
2Solution 1.0mL mixing is placed 6min, adds 10% AL (NO
2)
3Solution 1.0mL places 6min, then adds 4% NaOH solution 10mL, and adding distil water is diluted to scale and shakes up and place 15min; Not add rutin as blank, measure respectively its absorbance (A) under wavelength 510nm; Take concentration C as abscissa, absorbance A is vertical coordinate, gets regression equation: A=12.02C-0.0110, and r=0.9997, concentration C and absorbance A are good in 8.04-80.4 μ g/mL scope internal linear;
4, assay: the accurate sample solution 1.0mL that draws, adding distil water is measured absorbance to 6mL by the condition under " 3 ", and parallel assay 3 times is by the content of total flavones in regression equation calculation snow chrysanthemum.
A kind of preparation method and purposes of avenging total flavones in chrysanthemum of the present invention, its advantage is: the present invention has overcome the deficiency of existing extraction and separation technology, adopting membrane separation technique associating double water phase separation technology that snow chrysanthemum extract has been carried out extracting separates, membrane separation technique and double water phase separation technology belong to physical separation method, neither destroy material composition, selective again, be a kind of low energy consumption, oligosaprobic separation method.The method has improved the content of target component-total flavones, has removed most of invalid components, and the active component of product has obtained effective enrichment.The present invention is because have higher selectivity to separate targets, thus can change micro-filtration membrane, ultrafilter membrane and NF membrane specification and operating condition according to the needs of reality to product, thus control easily the composition of gained target product.With method provided by the invention separation and purification total flavones from the snow chrysanthemum, the effective ingredient accumulation rate is high, and loss is few, in product, general flavone content reaches more than 90%, under the prerequisite that does not change the effective ingredient structure, has shortened the production cycle, reduced energy consumption, product quality is greatly improved.Method provided by the present invention has that separating step is few, operating condition is gentle is easy to that technique is amplified and the advantages such as continued operation, is a kind of environmental type abstraction technique.
Description of drawings
Fig. 1 is the oxidation resistance figure of different quality mark snow chrysanthemum total flavones of the present invention, wherein-◆-be snow chrysanthemum total flavones ,-■-be Vc;
Fig. 2 is the activity figure of different quality mark snow chrysanthemum total flavones restraint of tyrosinase of the present invention, wherein-◆-be snow chrysanthemum total flavones ,-■-be arbutin.
The specific embodiment
Further illustrate the present invention below in conjunction with the specific embodiment, but be not limited to following embodiment;
Get dry snow inulin broken, cross 20 mesh sieves, get 2kg snow chrysanthemum medicated powder, adding 10 times of amount concentration is 60% alcoholic solution reflux 1 hour, extract 2 times, merge extractive liquid, filters, reclaim ethanol, and be concentrated into that to measure relative density when temperature 50 C be 1.10-1.20, obtain concentrated solution;
Be medical material with the concentrated solution dilute with water
:Medicinal liquid=1
:1 solution (refers to concentrated solution is diluted with water to the solution that equates with the medical material bulking value, it is the 100g medical material, concentrated solution is diluted to 100ml), standing, filter, filtrate is with 15000 rev/mins of centrifugalize, get centrifugal rear gained supernatant, adopt the micro-filtration membrane of aperture 0.02 μ m to filter except heavy, transmembrane pressure is 0.5Mpa, feed temperature is 25 ℃, obtains permeate;
Be 2000 ultrafilter membrane ultrafiltration with gained permeate molecular cut off, transmembrane pressure 0.5Mpa, feed temperature is 25 ℃, obtains ultrafiltrate;
It is that 200 hollow fiber nanofiltration membranes separate that ultrafiltrate is held back molecule again, transmembrane pressure 1.0Mpa, and feed temperature is 25 ℃, obtains concentrated solution, then concentrating under reduced pressure, the dry decorating film that gets;
Decorating film is adopted aqueous two-phase extraction, two phase aqueous extraction system is than pressing 5mL ethanol+5mL water+2g ammonium sulfate+0.15g decorating film mix homogeneously, centrifugal 2000 rev/mins of room temperatures, it is biphase that double-aqueous phase system is divided into the up and down, get the phase concentrating under reduced pressure, drying can obtain avenging the chrysanthemum total flavones, and this product contains total flavones (take the rutin note) as 93.5%.
Embodiment 2
Get dry snow inulin broken, cross 40 mesh sieves, get 2kg snow chrysanthemum medicated powder, adding 9 times of amount concentration is 70% alcoholic solution reflux 2 hours, extract 2 times, merge extractive liquid, filters, reclaim ethanol, and be concentrated into that to measure relative density when temperature 50 C be 1.10-1.20, obtain concentrated solution;
Be medical material with the concentrated solution dilute with water
:Medicinal liquid=1
:1 solution, standing, to filter, filtrate is got centrifugal rear gained supernatant with 15000 rev/mins of centrifugalize, filters except heavy with the micro-filtration membrane of aperture 0.04 μ m, and transmembrane pressure is 1.0Mpa, and 30 ℃ of feed temperatures obtain permeate;
Be 4000 ultrafilter membrane ultrafiltration with gained permeate molecular cut off, transmembrane pressure 1.0Mpa, 30 ℃ of feed temperatures obtain ultrafiltrate;
It is that 400 hollow fiber nanofiltration membrane separates that ultrafiltrate is carried out molecular cut off again, transmembrane pressure 2.0Mpa, and 30 ℃ of feed temperatures get concentrated solution, then concentrating under reduced pressure, dry decorating film;
Decorating film is adopted aqueous two-phase extraction, two phase aqueous extraction system is than pressing 4mL ethanol+6mL water+3g ammonium sulfate+0.15g decorating film mix homogeneously, centrifugal 3000 rev/mins of room temperatures, it is biphase that double-aqueous phase system is divided into the up and down, get the phase concentrating under reduced pressure, drying can obtain avenging the chrysanthemum total flavones, and this product contains total flavones (take the rutin note) as 96.7%.
Embodiment 3
Get dry snow inulin broken, cross 60 mesh sieves, get 2kg snow chrysanthemum medicated powder, adding 8 times of amount concentration is 80% alcoholic solution reflux 1 hour, extract 3 times, merge extractive liquid, filters, reclaim ethanol, and be concentrated into that to measure relative density when temperature 50 C be 1.10～1.20, obtain concentrated solution;
Be medical material with the concentrated solution dilute with water
:Medicinal liquid=1
:1 solution, standing, to filter, filtrate is got centrifugal rear gained supernatant with 15000 rev/mins of centrifugalize, filters except heavy with the micro-filtration membrane of aperture 0.08 μ m, and transmembrane pressure is 1.0Mpa, and 35 ℃ of feed temperatures obtain permeate;
Be 8000 ultrafilter membrane ultrafiltration with gained permeate molecular cut off, transmembrane pressure 1.0Mpa, 35 ℃ of feed temperatures obtain ultrafiltrate;
It is that 600 hollow fiber nanofiltration membrane separates that ultrafiltrate is carried out molecular cut off again, transmembrane pressure 1.0Mpa, and 35 ℃ of feed temperatures get concentrated solution, then concentrating under reduced pressure, dry decorating film;
Decorating film is adopted aqueous two-phase extraction, two phase aqueous extraction system is than pressing 3mL ethanol+7mL water+4g ammonium sulfate+0.15g decorating film mix homogeneously, centrifugal 4000 rev/mins of room temperatures, it is biphase that double-aqueous phase system is divided into the up and down, get the phase concentrating under reduced pressure, drying can obtain avenging the chrysanthemum total flavones, and this product contains total flavones (take the rutin note) as 95.9%.
Embodiment 4
The snow chrysanthemum total flavones that obtains by the method for the invention confirms that through test snow chrysanthemum total flavones has the effect of removing oxygen-derived free radicals resisting oxidation and delaying senility and whitening, and for the application at medicine and cosmetic industry after it provides foundation, relevant experimental data is as follows:
1 material:
1.1 reagent:
Embodiment 1-embodiment 4 is obtained any one snow chrysanthemum total flavones; 1,1-diphenyl-2-trinitrophenyl-hydrazine (DPPH), analytical pure, chemical industry company limited far away in the permanent industry in Beijing; Tryrosinase, chemical industry company limited far away in the permanent industry in Beijing; Arbutin and vitamin V c, analytical pure, source, Shanghai leaf bio tech ltd;
1.2 key instrument:
UV5500 type ultraviolet-visible spectrophotometer, Instr Ltd. is analysed by Shanghai unit;
2 methods:
2.1 removing the free radical oxidation resistance detects:
1,1-diphenyl-2-trinitrophenyl-hydrazine (DPPH) is a kind of stable organic free radical, maximum absorption wavelength is 516 nm, in alcoholic solution, each DPPH can generate a stable nitrogen free radical that contains, and has typical purple, when it with provide the free radical scavenger of 1 electronics to do the used time, generate colourless product, the typical purple of solution is shoaled, its fading extent becomes stoichiometric relationship with the sharing electron number; Thereby can carry out quantitative analysis with spectrophotography and detect the free radical scavenging situation, thereby the oxidation resistance of assess sample; Its oxidation resistance represents with clearance rate, and clearance rate is larger, shows that its oxidation resistance is stronger; Experiment detects the oxidation resistance of snow chrysanthemum total flavones by the DPPH method, with the positive contrast of standard Vc, compare the size of their oxidation resistances;
The configuration of testing sample solution: will remove free radical antioxidative active component (snow chrysanthemum total flavones and V
C) to be mixed with mass fraction with deionized water respectively be 0.2%, 0.4%, 0.6%, 0.8%, 1.0% solution, standby; Every group of sample solution parallel laboratory test 3 times, test specimens 1:2960 μ L DPPH solution (1 * 10
-4The mol/L ethanol solution) with 40 μ L testing sample solutions; Test specimens 2:2960 μ L DPPH solution (1 * 10
-4The mol/L ethanol solution) with 40 μ L deionized waters; Test specimens 3:2960 μ L dehydrated alcohol and 40 μ L testing sample solutions;
After 3 specimen liquid prepare simultaneously, at 25 ℃ of lower isothermal reaction 30 min of temperature, be transferred in the 3mL cuvette, in 516 nm place's survey absorbance A, calculate as follows the clearance rate to free radical:
Clearance rate=[(A
2+ A
3-A
1)/A
2] * 100%
A in formula
1Absorbance for the testing sample group; A
2Absorbance for the blank group; A
3Be testing sample group self absorbance in reaction system;
2.2 white-skinned face function detects:
Tryrosinase is the rate-limiting enzyme of melanocyte synthetic reaction, has tyrosine hydroxyl enzyme and DOPA hydroxyl enzyme two aspects activity.Sample is better to tyrosinase inhibitory action, and whitening effect is more obvious.Tyrosinase catalysis tyrosine generates DOPA, DOPA transforms and generates the DOPA quinone, the DOPA quinone is bronzing, at 475 nm places, obtained the maximum absorption is arranged, utilize spectrophotometry to add whitening agent and do not add the variation that generates DOPA quinone amount in the whitening agent reaction and detect whitening agent to the suppression ratio of tryrosinase; Experiment detects snow chrysanthemum total flavones white-skinned face function by the method for restraint of tyrosinase activity, with the positive contrast of arbutin, compares the size of their white-skinned face functions; The configuration of testing sample solution: it is 0.5%, 1.0%, 1.5%, 2.0% that the active component of restraint of tyrosinase (snow chrysanthemum total flavones and arbutin) is mixed with mass fraction with deionized water respectively, and 2.5%, 3.0% solution is standby; Every group of sample solution parallel laboratory test 3 times, the TYR solution of test specimens 1:0.5 mL (mass fraction 0.1%)+2.0 mL phosphate buffer (pH=6.8)+0.5 mL tryrosinase solution (with the phosphate buffer configuration, mass concentration is 200 μ g/mL); Test specimens 2:2.5 mL phosphate buffer (pH:6.8)+0.5 mL tryrosinase solution (with the phosphate buffer configuration, mass concentration is 200 μ g/mL); The TYR solution of test specimens 3:0.5 mL (mass fraction 0.1%)+0.5 mL specimen solution+1.5 mL phosphate buffer (pH=6.8)+0.5 mL tryrosinase solution (with the phosphate buffer configuration, mass concentration is 200 μ g/mL); The specimen solution of test specimens 4:0.5 mL+2.0 mL phosphate buffer (pH=6.8)+0.5 mL tryrosinase solution (with the phosphate buffer configuration, mass concentration is 200 μ g/mL).After 4 specimen liquid prepare simultaneously, at 25 ℃ of lower isothermal reaction 3.5 h of temperature, be transferred in 3 mL cuvettes, in 475 nm place's survey absorbance A, calculate as follows the suppression ratio to tryrosinase:
Suppression ratio=[1-(A
3One A
4)/(A
1One A
2)] * 100%
A in formula
1Absorbance for the blank group; A
2Absorbance for reaction system self; A
3Absorbance for the testing sample group; A
4For treating test sample group self absorbance in reaction system;
3 results:
3.1 remove free radical oxidation resistance experimental result:
Snow chrysanthemum total flavones and Vc solution removal free radical oxidation resistance relatively see Fig. 1, as can be seen from Figure 1, snow chrysanthemum total flavones has external removing free radical antioxidative ability, and clearance rate and the concentration of avenging the chrysanthemum total flavones become positive correlation; In pharmaceuticals, generally adopt one of the strongest material of removing free radical activity Vc to estimate certain material as positive control and remove free radical antioxidative ability, remove effect more near Vc, show that this material is removed free radical antioxidative effect better; As can be seen from Figure 1, to be 0.4% snow chrysanthemum total flavones almost reached to the clearance rate of free radical the level of descending Vc solution with concentration to mass fraction, shows that snow chrysanthemum total flavones has good antioxygenic property, and the pharmaceutical raw material medicine that can be used as slow down aging uses.
3.2 white-skinned face function experimental result:
Snow chrysanthemum total flavones and arbutin solution white-skinned face function more as shown in Figure 2, as can be seen from Figure 2, snow chrysanthemum total flavones has the ability of vitro inhibition tyrosinase activity, and suppression ratio and the concentration of avenging the chrysanthemum total flavones become positive correlation; Arbutin is generally acknowledge in the world a kind of can restraint of tyrosinase active, material with white-skinned face function, it can be under lower concentration the effective activity of restraint of tyrosinase, in blocking-up skin, tyrosine changes melanin under the catalysis of tryrosinase, thereby reduce melanic generation, reach the effect of whitening; Therefore, in cosmetic industry generally with arbutin as the right effect of the positive more near arbutin, show that this material restraint of tyrosinase effect is better, thereby reducing melanin forms, reach whitening effect, as can be seen from Figure 2, the ability of snow chrysanthemum total flavones restraint of tyrosinase is basic approaching with the arbutin solution with concentration, proof snow chrysanthemum total flavones has certain white-skinned face function, can be used as the skin-lightening cosmetic additive and uses.
Claims (6)
1. preparation method of avenging the chrysanthemum total flavones is characterized in that following these steps to carrying out:
A, the broken 20-60 mesh sieve of crossing of the snow inulin of drying is become medicated powder, the concentration of doubly measuring with 8-10 is alcohol heating reflux 1-2 hour of 60-80%, extracts 2-3 time, merge extractive liquid, filters, and reclaims ethanol, and be concentrated into when temperature 50 C that to measure relative density be 1.10-1.20, obtain concentrated solution;
B, with the 1:1 dilution by volume of step a concentrated solution water, standing, filter, filtrate is got centrifugal rear gained supernatant with 15000 rev/mins of centrifugalize, then filters except heavy with the micro-filtration membrane of aperture 0.02-0.1 μ m, transmembrane pressure is 0.5Mpa-1.0Mpa, feed temperature 25-35 ℃, obtains permeate;
C, the permeate of step b is carried out hollow fiber ultrafiltration membrane separate, the little 0.5Mpa-1.0Mpa of transmembrane pressure, feed temperature is 25-35 ℃, obtains ultrafiltrate;
D, the ultrafiltrate of step c is carried out hollow fiber nanofiltration membrane again separate, transmembrane pressure is 1.0Mpa-2.0Mpa, and feed temperature is 25-35 ℃, gets concentrated solution;
E, with steps d gained concentrated solution concentrating under reduced pressure, dry decorating film;
F, the decorating film of step e is adopted aqueous two-phase extraction, room temperature 2000-4000 rev/min centrifugal, it is biphase that double-aqueous phase system is divided into the up and down, gets the phase concentrating under reduced pressure, drying can obtain avenging the chrysanthemum total flavones.
2. want 1 described method according to right, the molecular cut off that it is characterized in that step c hollow fiber ultrafiltration membrane is 2000-8000.
3. want 1 described method according to right, the molecular cut off that it is characterized in that the steps d hollow fiber nanofiltration membrane is 200-600.
4. want 1 described method according to right, the system that it is characterized in that step f aqueous two-phase extraction is by 3-5mL ethanol+5-7mL water+2-4g ammonium sulfate+0.15g decorating film mix homogeneously.
5. a right is wanted the purposes of snow chrysanthemum total flavones in the medicine of preparation antioxidant that 1 described method obtains.
6. a right is wanted the purposes of snow chrysanthemum total flavones in preparation adornment product industry that 1 described method obtains.
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| CN104000754A (en) * | 2014-05-08 | 2014-08-27 | 上海应用技术学院 | Mask containing Kunlun snow Chrysanthemum petals and preparation method thereof |
| CN108703912A (en) * | 2018-08-10 | 2018-10-26 | 广州华玺生物科技有限公司 | A kind of snow chrysanthemum flavonoids extract and preparation method thereof and the application in cosmetics |
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