CN103074397A - Method for preparing fructo oligosaccharides by using sugarcane juices - Google Patents
Method for preparing fructo oligosaccharides by using sugarcane juices Download PDFInfo
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Abstract
The invention discloses a method for preparing fructo oligosaccharides by using sugarcane juices as raw materials. The method mainly comprises the following steps: extracting juices from sugarcanes, clarifying the juices, evaporating the juices, fumigating the juices by using sulfur, crystallizing the juices, separating honey from the juices, washing the honey, remelting the honey, filtering the honey through a granular active carbon to remove impurities, decoloring the honey through an ion exchange resin so as to prepare a refined sugar syrup, and converting, decoloring and concentrating the refined sugar syrup by using an immobilized fructosyl transferase column so as to prepare a sugar syrup with fructo oligosaccharide content of more than 55 %. According to the invention, traditional processes such as a white sugar drying process, a refined sugar crystallizing process and a refined sugar dissolving process are eliminated; and moreover, as the fructo oligosaccharides are prepared by using the fructosyl transferase column, enzyme separation treatment is not needed, so that the whole technical process is reduced, the production time is obviously reduced and the product purity is high. The cost of the whole production process is greatly reduced and the advantages of time conservation, labor conservation and continuous production are obtained.
Description
Technical field
The present invention relates to the preparation method of oligofructose; specifically take sugar cane juice as raw material; make affination syrup through clarification, evaporation, stove drying, crystallization, purging, after washing honey, Hui Rong, gac filtration removal of impurities, ion-exchange resin decolorization, after prepare again the method for oligofructose through the conversion of immobilized fructosyltransferase post, decolouring, concentration process.
Background technology
(English name is oligofructose: Fructooligosaccharidc, FOS), claim again oligofructose, FOS etc., molecular formula is that G-F-Fn(G is glucose, F is fructose, n=1 ~ 3), be with the general name of β-1,2 glycosidic link in conjunction with the formed kestose of several D-Fructoses, GF3, GF4 and composition thereof on the sucrose molecules.
FOS is present in natural phant and some herbaceous plant more, as: daily edible vegetables and fruit, especially with jerusalem artichoke (16%～20%), asparagus (1%～20%), witloof (5%～10%), garlic (3%～6%), onion (2%～6%), leek (2%～5%), burdock (3.6%), wheat class (1%～4%), and content is higher in honey and the banana, also contain on a small quantity in rye, the tomato, but therefrom extraction is very difficult, the lower and very difficult realization suitability for industrialized production of yield.
Because the nourishing function that oligofructose is good and special physiologically active, as a kind of internationally recognized typical prebiotics representative, its edible safety has obtained the affirmation of government in many countries, extremely modern food manufacturing enterprise and human consumer favor for it.It has following good nourishing function and physiology physical property:
1, optimizes the gi tract bacterium colony.Through for many years research, people recognize that gradually bifidus bacillus is to follow the animal intestinal beneficial bacterium in all one's life.The bifidobacterium fermentation oligofructose produces short chain fatty acid and some antibiotic, thereby can suppress the breeding of intrinsic putrefactive bacterium in external source pathogenic bacterium and the intestines, reduces the generation of poisonous tunning and the propagation of unwanted bacteria.The minimizing that the toxic metabolite product forms can alleviate the burden that liver divides toxolysin greatly, thus indirect protection liver;
2, promote enteron aisle metabolism campaign.Oligofructose can promote small intestinal peristalsis, accelerates degraded and the eliminating of septic matter in the enteron aisle; Have relaxing bowel, improve the effect of ight soil proterties, prevention and alleviate constipation.In addition, the oligofructose water soluble, easily edible, do not absorbed by body and harmful bacteria, can form the rectum defecation reflex, with bifidus bacillus synergy is arranged, can play the effect of toxin expelling and detoxifcation.The toxin expelling mode of this microecological balance is fool proof effectively, and is without any side effects;
3, enhancing body immunity.FOS is by the propagation bifidus bacillus, its cell walls and secretory product can produce a large amount of phylactic agents, such as the S-TGA immune protein, it stops bacterial adhesion in the ability of host's intestinal submucosa tissue, and in addition, FOS can combine with toxin, virus and direct cell surface, in conjunction with after, FOS can slow down the absorption of antigen as the auxiliary agent of exogenous antigen, thereby improves the cell of body and the immunologic function of body fluid;
4, low carious tooth.FOS can not be generated insoluble glucan as fermentation substrate by streptococcus mutans, the place (tartar) that deposition is not provided, produces acid and corrode for oral microorganism, and the lactic acid quantity that is generated by FOS is below 50% of sucrose.
5, blood fat reducing and cholesterol.FOS is a kind of water-soluble dietary fibre, can play the effect that reduces serum cholesterol and blood fat, and its mechanism is that FOS passes through bifidobacterium fermentation in the intestines, produce propionic acid, hinder the synthetic of cholesterol, impel cholesterol to change to bile acide, increase the bile acide output, thereby can blood fat reducing.
In the prior art, disclose a kind ofly take sucrose as raw material such as Chinese patent application 96106345.9, the Qu thalline that adding can produce the fructose transferase reacts, through decolouring desalination, the concentrated oligofructose that makes; It is oligofructose that another Chinese patent application 201010548887.X discloses employing fructosyl transferase conversion sucrose.This shows, the production of oligofructose is as raw material basically take refined sucrose.At present, cane sugar manufacture has two kinds of patterns, and the one, with sulfurous method or carbonatation process direct production white sugar, another is to make raw sugar with lime method first, then at sugar refinery again Hui Rong, defecation, crystallization becomes refined sugar again.Utilize at last refined sugar to go out oligofructose through fructosyl transferase effect final production.Under this pattern, producing white sugar needs through super-dry, and producing refined sugar needs back again molten white sugar, purification, crystallization, need dissolve refined sugar and produce oligofructose, causes complex process and energy dissipation, is unfavorable for serialization production.
In addition, the method for utilizing at present sugar cane juice to produce oligofructose has no relevant report.The present invention has overcome above shortcoming, utilizes sugar cane juice to prepare oligofructose, and production cost greatly reduces, have save time, laborsaving, the simple advantage of technique, for future large-scale industrial production lay the foundation.
Summary of the invention
An object of the present invention is to provide a kind of method for preparing oligofructose with sugar cane juice, it is characterized in that may further comprise the steps:
A) extract, clarify and the evaporation sugarcane juice: concentrated sugarcane juice, make its concentration reach 65 ~ 70 ° of Brix, obtain the raw sugar slurry;
B) stove drying: utilize pH value and the viscosity of sulfurous gas reduction syrup, wherein between pH to 6.0 ~ 7.0;
C) cane sugar boiling and crystal and purging, wash honey;
D) Hui Rong: to add water Hui Rong be 50 ~ 60 ° of Brix to concentration to the gained crystal after will washing honey;
E) activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed, and the gac add-on is 0.6 ~ 1.5% of Sucrose Content, and bleaching temperature is 50 ~ 85 ℃, and bleaching time is 15 ~ 90min;
F) ion-exchange resin decolorization: male-female ion exchange resin carries out desalination bleaching to syrup, obtains affination syrup;
G) enzymolysis: use fructosyl transferase that affination syrup is carried out enzymolysis;
H) obtain product through decolouring, removal of impurities, concentrated, drying.
Replenish as a kind of of above technical scheme, preferably, the sugarcane stem cut fragmentate, or sugarcane is torn, utilize multiple squeezing machine to extract sugarcane juice, the sugar cane juice that obtains after adopting high-efficiency evaporator to clarifying treatment concentrates.
Replenish as a kind of of above technical scheme, preferably, syrup is transferred in the crystallizer, adopt the saturation steam cane sugar boiling and crystal; Utilize discontinuous centrifuge that massecuite is carried out purging, then distinguish water, vapour washing crystal, the gained crystal is granulated sugar.
Replenish as a kind of of above technical scheme, preferably, oligofructose solution through the disc type diatomite filter decolour, filtering and impurity removing, wherein, filter pressure is at 0.2 ~ 0.25 Mpa; Under the vacuum condition of-0.08 to-0.09 Mpa, vaporization temperature reaches 70 ~ 75 ℃ and carries out the concentrated and vacuum-drying of multiple-effect vacuum and obtain product.
One of them aspect of the present invention is, adopts the following steps rclarifying cane juice: passing into calcium hydroxide, to make pH be 10.5 ~ 11.5, and adjust the temperature to 45 ~ 55 ℃, and the pre-ash time is 5 ~ 10min approximately, continues to add calcium hydroxide to pH=12.5 ~ 13.5 after pre-ash is processed; For the first time flocculation, filtration: pass into CO
2Gas, neutralization solution to pH value is 10 ~ 11, and temperature is controlled at 50 ~ 60 ℃, and the time is that 10 ~ 15min produces cotton-shaped calcium carbonate, adds simultaneously polyacrylamide flocculant, precipitation centrifuging; For the second time flocculation, filtration: pass into CO
2Gas, neutralization solution to pH value is 7.5 ~ 8.5, and temperature is controlled at 60 ~ 70 ℃, and the time is that 5 ~ 15min produces cotton-shaped calcium carbonate, precipitation centrifuging; Two clear juice stove dryings: utilize sulfurous gas to reduce between the pH value to 6.5 of syrup ~ 7.5 sugarcane juice that obtains clarifying.
Another aspect of the present invention is, the rclarifying cane juice step is characterised in that, described polyacrylamide flocculant addition is 2 ~ 2.5 mg/kg to the sugarcane ratio.
Another aspect of the present invention is, the product that obtains is soluble solid, and concentration is 65 ~ 75%, purity is greater than 55% oligofructose slurry.
Another aspect of the present invention is, the product that obtains is moisture content less than 5% solid oligofructose powder.
Randomly, the cane sugar boiling and crystal step among each embodiment of the present invention can adopt nature to be cooled to 45 ℃ in the latter stage of boiling sugar, grows up to promote crystal.
Randomly, the enzymolysis among each embodiment of the present invention can use the immobilized fructosyltransferase post that affination syrup is carried out enzymolysis, and temperature of reaction is 40 ~ 60 ℃, and the pH value is 5 ~ 7, and the time is 6 ~ 12h.
The present invention with compare as the raw material production oligofructose take sucrose, have the following advantages:
1, production cost is low.The invention provides the novel process for preparing oligofructose take sugar cane juice as raw material, saved traditional white sugar drying and the process of refined sugar crystallization and dissolving, the sucrose composition is fully used.Utilize the fructosyl transferase post to prepare oligofructose, shortened whole technical process, need not to carry out the enzyme separating treatment, the production time obviously shortens, and product purity is high.Whole production technique cost greatly reduces, and have save time, laborsaving, the advantage that can be continuously produced, for future the large-scale industrial production oligofructose lay a good foundation.
2, be fit to large-scale industrial production.The characteristics of the inventive method are directly to utilize sugar cane juice to prepare oligofructose, have saved traditional sugaring process, and a direct step finishes, and shortened the process of producing, and whole flow operations is simple, meets the requirement of large-scale industrial production.
3, the method for innovative usage activated carbon filtration of the present invention removal of impurities and ion-exchange resin decolorization prepares affination syrup, greatly improved refining efficient, and the process that has shortened whole preparation, cost and the time of whole technique have been saved, avoided simultaneously the participation of chemical reagent in the treating process, reduced follow-up treatment step, more healthy refined sugar is provided.
The drawings and specific embodiments illustrate these and other embodiment of the present invention in further detail.
Description of drawings
By following explanation and relevant drawings can better understand the present invention on address further advantage, wherein:
Fig. 1 is process flow sheet for preparing oligofructose embodiment with sugar cane juice of the present invention.
Embodiment
It should be noted, the intent of the present invention is not to be limited to specifically described embodiment.On the contrary, the present invention is contained all transformations that belong to aim of the present invention and scope, is equal to and replacement scheme.
The object of the present invention is to provide a kind ofly take sugar cane juice as raw material, prepare the method for oligofructose, complicated to solve prior art processes, the problem such as production cost is high, and resource and energy dissipation are large is beneficial to serialization production.
The technical solution adopted for the present invention to solve the technical problems is:
Take sugar cane juice as raw material, cane milling extraction of the juice, clarification, evaporation, stove drying, crystallization, purging, the techniques such as decolouring, filtering and impurity removing and concentration of washing honey, Hui Rong, gac filtration removal of impurities, ion-exchange resin decolorization, immobilized fructosyltransferase post enzymolysis and oligofructose solution are obtained the oligofructose product.
Embodiment 1:
Extract sugarcane juice: utilize multiple squeezing machine that sugarcane is carried out squeeze juice extracting.First the sugarcane stem is cut and fragmentated, or sugarcane is torn, extract sugarcane juice by squeezing machine.For helping to extract sugarcane juice, a small amount of water is sprayed in the bagasse impel Sucrose to leach through squeezing again, maximally utilise starting material, obtain Cane Mixed Juice.
Rclarifying cane juice: sugar cane juice is processed through the pre-ash of calcium hydroxide first, makes pH value of solution=11, and adjusts the temperature to 45 ℃, and the pre-ash time is 5min approximately.After processing, pre-ash continues to add calcium hydroxide to pH=12.5, for the first time the logical CO of putting
2Gas, neutralization makes pH value of solution=11, temperature be controlled at 50 ℃, and the time is 10min, and produce cotton-shaped calcium carbonate this moment, adds simultaneously polyacrylamide flocculant (addition is 2 mg/kg to the sugarcane ratio), precipitates centrifuging for the first time.Then lead to for the second time and put CO
2Gas, neutralization makes pH value of solution=7.5, temperature be controlled at 60 ℃, and the time is 10min, precipitates centrifuging for the second time.
Because secondary CO
2The pH value of the sugar cane juice after full charge is filtered is higher, need carry out two clear juice stove dryings, reduces the pH of two clear juice, and the decomposition that slows down reducing sugar suppresses the generation of pigment.After utilizing sulfurous gas that two clear juice are carried out stove drying, the pH value is controlled between 6.5 ~ 7.5.
The evaporation sugarcane juice: the clear juice that obtains after clarifying treatment adopts high-efficiency evaporator concentrated, makes its concentration reach 65 ° of Brix, obtains the raw sugar slurry, to satisfy follow-up requirement of boiling sugar.
Stove drying: utilize sulfurous gas can reduce pH value and the viscosity of syrup, reduce the syrup colour.Utilize the titanium dioxide stove drying to syrup pH=6.0, obtain clearly syrup.
Cane sugar boiling and crystal: the purpose of boiling sugar is to greatest extent the sucrose in the liquid glucose to be formed crystal and extract, and reduces the sugar that waste molasses is taken away, thereby economizes in raw materials, and saves cost.Syrup is transferred in the crystallizer, is 8.7 * 10 in vacuum tightness
4Handkerchief, vapor pressure 3 * 10
5Under the handkerchief, adopt saturation steam to boil sugar.Randomly, because in the latter stage of boiling sugar, the concentration of syrup is high, viscosity is large, convection current is slow, should not continue boiling in crystallizer.Therefore, can adopt the method for nature cooling in the latter stage of boiling sugar, syrup is cooled to about 45 ℃, just can carry out next step purging step.
Purging, wash honey: utilize discontinuous centrifuge that massecuite is carried out purging, then water, vapour washing crystal are arranged respectively.
Hui Rong: it is 55 ° of Brix to concentration that the gained crystal is added water Hui Rong.
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed.The gac add-on is 1% of Sucrose Content, and bleaching temperature is 50 ℃, and bleaching time is 30min.
Ion-exchange resin decolorization: syrup carries out desalination, decolouring through macroporous strong basic styrene series male-female ion exchange resin again, obtains affination syrup.
Enzymolysis: use the immobilized fructosyltransferase post.Randomly, 40 ℃ of enzyme digestion reaction temperature, pH value are 5, and enzymolysis time is 6h.
Decolouring, removal of impurities: oligofructose solution carries out decolorization filtering through the disc type diatomite filter, and filter pressure is at 0.2 ~ 0.25 Mpa, and filtrate is transported to vacuum concentrator.
Concentrated, dry: in the vacuum of-0.09 Mpa, vaporization temperature reaches under 70 ℃ of conditions, and concentrated filtrate to solid content is 65 ~ 75%, obtains purity greater than 55% oligofructose syrup.
Embodiment 2:
Extract sugarcane juice: utilize multiple squeezing machine that sugarcane is carried out squeeze juice extracting.First the sugarcane stem is cut and fragmentated, or sugarcane is torn, extract sugarcane juice by squeezing machine.For helping to extract sugarcane juice, a small amount of water is sprayed in the bagasse impel Sucrose to leach through squeezing again, maximally utilise starting material, obtain Cane Mixed Juice.
Rclarifying cane juice: sugar cane juice is processed through the pre-ash of calcium hydroxide first, makes pH value of solution=11, and adjusts the temperature to 55 ℃, and the pre-ash time is 5min approximately.After processing, pre-ash continues to add calcium hydroxide to pH=13, for the first time the logical CO of putting
2Gas, neutralization makes pH value of solution=11, temperature be controlled at 55 ℃, and the time is 14min, and produce cotton-shaped calcium carbonate this moment, adds simultaneously polyacrylamide flocculant (addition is 2.5 mg/kg to the sugarcane ratio), precipitates centrifuging for the first time.Then lead to for the second time and put CO
2Gas, neutralization makes pH value of solution=8.0, temperature be controlled at 65 ℃, and the time is 15min, precipitates centrifuging for the second time.
Because secondary CO
2The pH value of the sugar cane juice after full charge is filtered is higher, need carry out two clear juice stove dryings, reduces the pH of two clear juice, and the decomposition that slows down reducing sugar suppresses the generation of pigment.After utilizing sulfurous gas that two clear juice are carried out stove drying, the pH value is controlled between 6.5 ~ 7.5.
The evaporation sugarcane juice: the clear juice that obtains after clarifying treatment adopts high-efficiency evaporator concentrated, makes its concentration reach 65 ° of Brix, obtains the raw sugar slurry, to satisfy follow-up requirement of boiling sugar.
Stove drying: utilize sulfurous gas can reduce pH value and the viscosity of syrup, reduce the syrup colour.Utilize the titanium dioxide stove drying to syrup pH=6.5, obtain clearly syrup.
Cane sugar boiling and crystal: the purpose of boiling sugar is to greatest extent the sucrose in the liquid glucose to be formed crystal and extract, and reduces the sugar that waste molasses is taken away, thereby economizes in raw materials, and saves cost.Syrup is transferred in the crystallizer, is 8.7 * 10 in vacuum tightness
4Handkerchief, vapor pressure 4 * 10
5Under the handkerchief, adopt saturation steam to boil sugar.Randomly, because in the latter stage of boiling sugar, the concentration of syrup is high, viscosity is large, convection current is slow, should not continue boiling in crystallizer.Therefore, can adopt the method for nature cooling in the latter stage of boiling sugar, syrup is cooled to about 45 ℃, just can carry out next step purging step.
Purging, wash honey: utilize discontinuous centrifuge that massecuite is carried out purging, then water, vapour washing crystal are arranged respectively.
Hui Rong: it is 60 ° of Brix to concentration that the gained crystal is added water Hui Rong.
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed.The gac add-on is 1.5% of Sucrose Content, and bleaching temperature is 50 ℃, and bleaching time is 20min.
Ion-exchange resin decolorization: syrup carries out desalination, decolouring through macroporous strong basic styrene series male-female ion exchange resin again, obtains affination syrup.
Enzymolysis: use the immobilized fructosyltransferase post.Randomly, the enzyme digestion reaction temperature 45 C, the pH value is 5.5, enzymolysis time is 8h.
Decolouring, removal of impurities: oligofructose solution carries out decolorization filtering through the disc type diatomite filter, and filter pressure is at 0.2 ~ 0.25 Mpa, and filtrate is transported to vacuum concentrator.
Concentrated, dry: in the vacuum of-0.09 Mpa, vaporization temperature reaches under 70 ℃ of conditions, and concentrated filtrate to solid content is 65 ~ 75%, obtains purity greater than 55% oligofructose syrup.
Embodiment 3:
Extract sugarcane juice: utilize multiple squeezing machine that sugarcane is carried out squeeze juice extracting.First the sugarcane stem is cut and fragmentated, or sugarcane is torn, extract sugarcane juice by squeezing machine.For helping to extract sugarcane juice, a small amount of water is sprayed in the bagasse impel Sucrose to leach through squeezing again, maximally utilise starting material, obtain Cane Mixed Juice.
Rclarifying cane juice: sugar cane juice is processed through the pre-ash of calcium hydroxide first, makes pH value of solution=11, and adjusts the temperature to 50 ℃, and the pre-ash time is 8min approximately.After processing, pre-ash continues to add calcium hydroxide to pH=13, for the first time the logical CO of putting
2Gas, neutralization makes pH value of solution=11, temperature be controlled at 60 ℃, and the time is 15min, and produce cotton-shaped calcium carbonate this moment, adds simultaneously polyacrylamide flocculant (addition is 2.5 mg/kg to the sugarcane ratio), precipitates centrifuging for the first time.Then lead to for the second time and put CO
2Gas, neutralization makes pH value of solution=8.5, temperature be controlled at 70 ℃, and the time is 15min, precipitates centrifuging for the second time.
Because secondary CO
2The pH value of the sugar cane juice after full charge is filtered is higher, need carry out two clear juice stove dryings, reduces the pH of two clear juice, and the decomposition that slows down reducing sugar suppresses the generation of pigment.After utilizing sulfurous gas that two clear juice are carried out stove drying, the pH value is controlled between 6.5 ~ 7.5.
The evaporation sugarcane juice: the clear juice that obtains after clarifying treatment adopts high-efficiency evaporator concentrated, makes its concentration reach 70 ° of Brix, obtains the raw sugar slurry, to satisfy follow-up requirement of boiling sugar.
Stove drying: utilize sulfurous gas can reduce pH value and the viscosity of syrup, reduce the syrup colour.Utilize the titanium dioxide stove drying to syrup pH=6.5, obtain clearly syrup.
Cane sugar boiling and crystal: the purpose of boiling sugar is to greatest extent the sucrose in the liquid glucose to be formed crystal and extract, and reduces the sugar that waste molasses is taken away, thereby economizes in raw materials, and saves cost.Syrup is transferred in the crystallizer, is 8.7 * 10 in vacuum tightness
4Handkerchief, vapor pressure 5 * 10
5Under the handkerchief, adopt saturation steam to boil sugar.Randomly, because in the latter stage of boiling sugar, the concentration of syrup is high, viscosity is large, convection current is slow, should not continue boiling in crystallizer.Therefore, can adopt the method for nature cooling in the latter stage of boiling sugar, syrup is cooled to about 45 ℃, just can carry out next step purging step.
Purging, wash honey: utilize discontinuous centrifuge that massecuite is carried out purging, then water, vapour washing crystal are arranged respectively.
Hui Rong: it is 60 ° of Brix to concentration that the gained crystal is added water Hui Rong.
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed.The gac add-on is 1.2% of Sucrose Content, and bleaching temperature is 70 ℃, and bleaching time is 40min.
Ion-exchange resin decolorization: syrup carries out desalination, decolouring through macroporous strong basic styrene series male-female ion exchange resin again, obtains affination syrup.
Enzymolysis: use the immobilized fructosyltransferase post.Randomly, the enzyme digestion reaction temperature 45 C, the pH value is 6.0, enzymolysis time is 12h.
Decolouring, removal of impurities: oligofructose solution carries out decolorization filtering through the disc type diatomite filter, and filter pressure is at 0.2 ~ 0.25 Mpa, and filtrate is transported to vacuum concentrator.
Concentrated, dry: in the vacuum of-0.08 Mpa, vaporization temperature reaches under 75 ℃ of conditions, and concentrated filtrate to solid content is 65 ~ 75%, obtains purity greater than 55% oligofructose syrup.Randomly, can carry out spray drying treatment, make moisture content less than 5% solid oligofructose powder.
Embodiment 4:
Extract sugarcane juice: utilize multiple squeezing machine that sugarcane is carried out squeeze juice extracting.First the sugarcane stem is cut and fragmentated, or sugarcane is torn, extract sugarcane juice by squeezing machine.For helping to extract sugarcane juice, a small amount of water is sprayed in the bagasse impel Sucrose to leach through squeezing again, maximally utilise starting material, obtain Cane Mixed Juice.
Rclarifying cane juice: sugar cane juice is processed through the pre-ash of calcium hydroxide first, makes pH value of solution=11.5, and adjusts the temperature to 45 ℃, and the pre-ash time is 10min approximately.After processing, pre-ash continues to add calcium hydroxide to pH=13.5, for the first time the logical CO of putting
2Gas, neutralization makes pH value of solution=10, temperature be controlled at 60 ℃, and the time is 15min, and produce cotton-shaped calcium carbonate this moment, adds simultaneously polyacrylamide flocculant (addition is 2.2mg/kg to the sugarcane ratio), precipitates centrifuging for the first time.Then lead to for the second time and put CO
2Gas, neutralization makes pH value of solution=8.0, temperature be controlled at 65 ℃, and the time is 10min, precipitates centrifuging for the second time.
Because secondary CO
2The pH value of the sugar cane juice after full charge is filtered is higher, need carry out two clear juice stove dryings, reduces the pH of two clear juice, and the decomposition that slows down reducing sugar suppresses the generation of pigment.After utilizing sulfurous gas that two clear juice are carried out stove drying, the pH value is controlled between 6.5 ~ 7.5.
The evaporation sugarcane juice: the clear juice that obtains after clarifying treatment adopts high-efficiency evaporator concentrated, makes its concentration reach 70 ° of Brix, obtains the raw sugar slurry, to satisfy follow-up requirement of boiling sugar.
Stove drying: utilize sulfurous gas can reduce pH value and the viscosity of syrup, reduce the syrup colour.Utilize the titanium dioxide stove drying to syrup pH=7.0, obtain clearly syrup.
Cane sugar boiling and crystal: the purpose of boiling sugar is to greatest extent the sucrose in the liquid glucose to be formed crystal and extract, and reduces the sugar that waste molasses is taken away, thereby economizes in raw materials, and saves cost.Syrup is transferred in the crystallizer, is 8.7 * 10 in vacuum tightness
4Handkerchief, vapor pressure 5 * 10
5Under the handkerchief, adopt saturation steam to boil sugar.Randomly, because in the latter stage of boiling sugar, the concentration of syrup is high, viscosity is large, convection current is slow, should not continue boiling in crystallizer.Therefore, can adopt the method for nature cooling in the latter stage of boiling sugar, syrup is cooled to about 45 ℃, just can carry out next step purging step.
Purging, wash honey: utilize discontinuous centrifuge that massecuite is carried out purging, then water, vapour washing crystal are arranged respectively.
Hui Rong: it is 60 ° of Brix to concentration that the gained crystal is added water Hui Rong.
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed.The gac add-on is 1.2% of Sucrose Content, and bleaching temperature is 60 ℃, and bleaching time is 60min.
Ion-exchange resin decolorization: syrup carries out desalination, decolouring through macroporous strong basic styrene series male-female ion exchange resin again, obtains affination syrup.
Enzymolysis: use the immobilized fructosyltransferase post.Randomly, 55 ℃ of enzyme digestion reaction temperature, pH value are 6.5, and enzymolysis time is 10h.
Decolouring, removal of impurities: oligofructose solution carries out decolorization filtering through the disc type diatomite filter, and filter pressure is at 0.2 ~ 0.25 Mpa, and filtrate is transported to vacuum concentrator.
Concentrated, dry: in the vacuum of-0.08 Mpa, vaporization temperature reaches under 75 ℃ of conditions, and concentrated filtrate to solid content is 65 ~ 75%, obtains purity greater than 55% oligofructose syrup.Randomly, can carry out spray drying treatment, make moisture content less than 5% solid oligofructose powder.
Embodiment 5:
Extract sugarcane juice: utilize multiple squeezing machine that sugarcane is carried out squeeze juice extracting.First the sugarcane stem is cut and fragmentated, or sugarcane is torn, extract sugarcane juice by squeezing machine.For helping to extract sugarcane juice, a small amount of water is sprayed in the bagasse impel Sucrose to leach through squeezing again, maximally utilise starting material, obtain Cane Mixed Juice.
Rclarifying cane juice: sugar cane juice is processed through the pre-ash of calcium hydroxide first, makes pH value of solution=11.5, and adjusts the temperature to 55 ℃, and the pre-ash time is 10min approximately.After processing, pre-ash continues to add calcium hydroxide to pH=13.5, for the first time the logical CO of putting
2Gas, neutralization makes pH value of solution=11, temperature be controlled at 60 ℃, and the time is 15min, and produce cotton-shaped calcium carbonate this moment, adds simultaneously polyacrylamide flocculant (addition is 2.5mg/kg to the sugarcane ratio), precipitates centrifuging for the first time.Then lead to for the second time and put CO
2Gas, neutralization makes pH value of solution=8.5, temperature be controlled at 70 ℃, and the time is 15min, precipitates centrifuging for the second time.
Because secondary CO
2The pH value of the sugar cane juice after full charge is filtered is higher, need carry out two clear juice stove dryings, reduces the pH of two clear juice, and the decomposition that slows down reducing sugar suppresses the generation of pigment.After utilizing sulfurous gas that two clear juice are carried out stove drying, the pH value is controlled between 6.5 ~ 7.5.
The evaporation sugarcane juice: the clear juice that obtains after clarifying treatment adopts high-efficiency evaporator concentrated, makes its concentration reach 70 ° of Brix, obtains the raw sugar slurry, to satisfy follow-up requirement of boiling sugar.
Stove drying: utilize sulfurous gas can reduce pH value and the viscosity of syrup, reduce the syrup colour.Utilize the titanium dioxide stove drying to syrup pH=7.0, obtain clearly syrup.
Cane sugar boiling and crystal: the purpose of boiling sugar is to greatest extent the sucrose in the liquid glucose to be formed crystal and extract, and reduces the sugar that waste molasses is taken away, thereby economizes in raw materials, and saves cost.Syrup is transferred in the crystallizer, is 8.7 * 10 in vacuum tightness
4Handkerchief, vapor pressure 5 * 10
5Under the handkerchief, adopt saturation steam to boil sugar.Randomly, because in the latter stage of boiling sugar, the concentration of syrup is high, viscosity is large, convection current is slow, should not continue boiling in crystallizer.Therefore, can adopt the method for nature cooling in the latter stage of boiling sugar, syrup is cooled to about 45 ℃, just can carry out next step purging step.
Purging, wash honey: utilize discontinuous centrifuge that massecuite is carried out purging, then water, vapour washing crystal are arranged respectively.
Hui Rong: it is 60 ° of Brix to concentration that the gained crystal is added water Hui Rong.
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed.The gac add-on is 1.5% of Sucrose Content, and bleaching temperature is 85 ℃, and bleaching time is 90min.
Ion-exchange resin decolorization: syrup carries out desalination, decolouring through macroporous strong basic styrene series male-female ion exchange resin again, obtains affination syrup.
Enzymolysis: use the immobilized fructosyltransferase post.Randomly, the enzyme digestion reaction temperature 60 C, the pH value is 7.0, enzymolysis time is 12h.
Decolouring, removal of impurities: oligofructose solution carries out decolorization filtering through the disc type diatomite filter, and filter pressure is at 0.2 ~ 0.25 Mpa, and filtrate is transported to vacuum concentrator.
Concentrated, dry: in the vacuum of-0.08 Mpa, vaporization temperature reaches under 75 ℃ of conditions, and concentrated filtrate to solid content is 65 ~ 75%, obtains purity greater than 55% oligofructose syrup.Randomly, can carry out spray drying treatment, make moisture content less than 5% solid oligofructose powder.
Claims (7)
1. method for preparing oligofructose with sugar cane juice is characterized in that may further comprise the steps:
Extract, clarify and the evaporation sugarcane juice: concentrated sugarcane juice, make its concentration reach 65 ~ 70 ° of Brix, obtain the raw sugar slurry;
Stove drying: utilize pH value and the viscosity of sulfurous gas reduction syrup, wherein between pH to 6.0 ~ 7.0;
Cane sugar boiling and crystal and purging, wash honey;
Hui Rong: to add water Hui Rong be 50 ~ 60 ° of Brix to concentration to the gained crystal after will washing honey;
The activated carbon filtration removal of impurities: retro-dissolved syrup carries out removal of impurities through granulated active carbon to be processed, and the gac add-on is 0.6 ~ 1.5% of Sucrose Content, and bleaching temperature is 50 ~ 85 ℃, and bleaching time is 15 ~ 90min;
Ion-exchange resin decolorization: male-female ion exchange resin carries out desalination bleaching to syrup, obtains affination syrup;
Enzymolysis: use fructosyl transferase that affination syrup is carried out enzymolysis;
Obtain product through decolouring, removal of impurities, concentrated, drying.
2. method according to claim 1 is characterized in that, described rclarifying cane juice step comprises:
Pre-ash is processed: passing into calcium hydroxide, to make pH be 10.5 ~ 11.5, and adjust the temperature to 45 ~ 55 ℃, and the pre-ash time is 5 ~ 10min approximately, continues to add calcium hydroxide to pH=12.5 ~ 13.5 after pre-ash is processed;
For the first time flocculation, filtration: pass into CO
2Gas, neutralization solution to pH value is 10 ~ 11, and temperature is controlled at 50 ~ 60 ℃, and the time is that 10 ~ 15min produces cotton-shaped calcium carbonate, adds simultaneously polyacrylamide flocculant, precipitation centrifuging;
For the second time flocculation, filtration: pass into CO
2Gas, neutralization solution to pH value is 7.5 ~ 8.5, and temperature is controlled at 60 ~ 70 ℃, and the time is that 5 ~ 15min produces cotton-shaped calcium carbonate, precipitation centrifuging;
Two clear juice stove dryings: utilize sulfurous gas to reduce between the pH value to 6.5 of syrup ~ 7.5.
3. method according to claim 2 is characterized in that described polyacrylamide flocculant addition is 2 ~ 2.5 mg/kg to the sugarcane ratio.
4. method according to claim 1, it is characterized in that: described product is soluble solid, concentration is 65 ~ 75%, purity is greater than 55% oligofructose slurry.
5. method according to claim 1 is characterized in that: described product is moisture content less than 5% solid oligofructose powder.
6. each described method according to claim 1-5 is characterized in that: described cane sugar boiling and crystal step is cooled to 45 ℃ to promote crystal to grow up naturally in the latter stage of boiling sugar.
7. each described method according to claim 1-5 is characterized in that: described enzymolysis uses the immobilized fructosyltransferase post that affination syrup is carried out enzymolysis, and temperature of reaction is 40 ~ 60 ℃, and the pH value is 5 ~ 7, and the time is 6 ~ 12h.
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| CN105886671A (en) * | 2016-02-26 | 2016-08-24 | 广西大学 | Clearing device for producing crystalline fructose with raw sugar as raw material |
| CN107574266A (en) * | 2017-10-12 | 2018-01-12 | 苏州昆蓝生物科技有限公司 | A kind of cane molasses decoloration process |
| CN108330150A (en) * | 2018-05-16 | 2018-07-27 | 量子高科(中国)生物股份有限公司 | A kind of oligofructose prepared as raw material using brown sugar |
| CN108546723A (en) * | 2018-04-20 | 2018-09-18 | 广西南宁大动保生物科技有限公司 | The method for preparing the feed grade fructooligosaccharide containing trace element as raw material using sugar-cane juice |
| CN110512032A (en) * | 2019-09-20 | 2019-11-29 | 贵州省好之味精品农业开发有限公司 | A kind of brown sugar tanning technique for refining sugar more efficient |
| CN113846134A (en) * | 2021-11-16 | 2021-12-28 | 广西大学 | Method for preparing fructo-oligosaccharide through biotransformation after cane molasses pretreatment |
| CN114032262A (en) * | 2021-11-09 | 2022-02-11 | 山东星光首创生物科技有限公司 | Method for producing sucrose tetrasaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105886671A (en) * | 2016-02-26 | 2016-08-24 | 广西大学 | Clearing device for producing crystalline fructose with raw sugar as raw material |
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| CN110512032A (en) * | 2019-09-20 | 2019-11-29 | 贵州省好之味精品农业开发有限公司 | A kind of brown sugar tanning technique for refining sugar more efficient |
| CN114032262A (en) * | 2021-11-09 | 2022-02-11 | 山东星光首创生物科技有限公司 | Method for producing sucrose tetrasaccharide |
| CN113846134A (en) * | 2021-11-16 | 2021-12-28 | 广西大学 | Method for preparing fructo-oligosaccharide through biotransformation after cane molasses pretreatment |
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