CN103074323A - Preparation method of immobilized crude oil degrading bacteria - Google Patents
Preparation method of immobilized crude oil degrading bacteria Download PDFInfo
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- CN103074323A CN103074323A CN2012105509729A CN201210550972A CN103074323A CN 103074323 A CN103074323 A CN 103074323A CN 2012105509729 A CN2012105509729 A CN 2012105509729A CN 201210550972 A CN201210550972 A CN 201210550972A CN 103074323 A CN103074323 A CN 103074323A
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Abstract
The invention relates to a preparation method of a petroleum degrading substance, and concretely relates to a preparation method of immobilized crude oil degrading bacteria. The preparation method of the immobilized crude oil degrading bacteria comprises the following steps: completely dissolving PVA (polyvinyl alcohol) at 80-100DEG C to prepare a 10% PVA solution, uniformly mixing the 10% PVA solution with a 1% SA (sodium alginate) solution, adding active carbon, uniformly mixing, cooling to 40DEG C, adding a bacterial liquid in a logarithmic phase, adding a saturated boric acid solution containing 3% of CaCl2 by a syringe in a dropwise manner, uniformly stirring at 0-4DEG C, carrying out heat insulation for 26h, filtering out micro-spheres, and washing by using normal saline. The immobilized micro-spheres prepared in the invention have the advantages of good mechanical strength, good elasticity, good permeability, high specific surface area, high pore volume, and dense pore structure.
Description
Technical field
The present invention relates to a kind of preparation method of oil degradation material.
Background technology
Oil causes the oil waste to the pollution of soil producing, in storing, refining processing and the use procedure, owing to reasons such as accident, upset operation and maintenance, overflowing and discharging of petroleum hydrocarbon can being arranged all inevitably.Present soil remediation technology both domestic and external mainly is divided into physical method, chemical process and biological treatment.Bioremediation technology comprises free bioremediation technology and immobilized bioremediation technology.So-called immobilization technology refers to utilize physics or chemical means with free microorganism or enzyme, and the area of space that is positioned to limit also makes it keep active and repeatedly used a kind of basic fundamental.At present, immobilized microorganism technique is widely used in environmental pollution improvement, and main improvement object is the waste water of unmanageable organic waste water and heavy metal contamination, and simultaneously research also relates to the Pollution abatement of atmosphere and soil.
How to fix, select which kind of carrier to make immobilized cell can keep intensity and activity, reduction cost the long period, and to prolong its work-ing life be the key that can immobilization technology widespread use in industrial production.In general, immobilized microorganism preparation method is divided into the 4 large classes such as absorption method, entrapping method, covalent coupling method and crosslinking, and wherein absorption method and entrapping method are the most commonly used.Entrapping method be utilize superpolymer when forming gel with cell embedding section within it, thereby reach the purpose of fixed cell.(PVA) is cheap for organic polymer carrier sodium alginate (SA), polyvinyl alcohol, and be little to the cell relative toxicity, mass-transfer performance good, is the fixed microorganism carrier material that is most widely used.Inorganic carrier such as porous Tao Zhu, gac etc., have physical strength large, to the microorganism nontoxicity, be difficult for by microorganism decomposition, acid and alkali-resistance, the characteristic such as cost is low, the life-span is long, thereby be the important solid support material of a class.
Summary of the invention
The present invention aims to provide the preparation method of a kind of microspheroidal immobilization oil degradation bacterium.
The preparation method of immobilization oil degradation bacterium of the present invention comprises:
With PVA fully dissolving under 80-100 ℃, make 10% solution, then mix with 1% SA solution, add again the gac mixing, be cooled to 40 ℃ after, add the bacterium liquid that is in logarithmic phase, then splash into syringe and contain 3% CaCl
2Saturated boric acid solution in, stir under 0-4 ℃, and be incubated 26 hours, filter out microballoon, and get final product with the physiological saline washing.
Preferably, the add-on of bacterium liquid is 20%.
Perhaps preferably, the add-on of gac is 50 g/L.
The immobilized microspheres of the present invention preparation has good physical strength, elasticity and perviousness, higher specific surface area and pore volume, fine and close pore texture; Wanting of the degradation rate specific ionization bacterium of immobilization bacterium is high, and the immobilization bacterium of spherical ratio sheet is high to the degradation rate of crude oil; Immobilization bacterium is stronger to the adaptability specific ionization bacterium of pH value, and activating 21 h, to be in the operating performance of immobilized microspheres of stationary phase best.
Embodiment
Embodiment one.
With 10 g PVA fully dissolving under 80-100 ℃, make 10% solution, then the SA solution with 100mL 1% mixes, add again 0.1 L gac mixing, after being cooled to 40 ℃, adding 20% is in the bacterium liquid of logarithmic phase, then splashes into syringe and contains 3% CaCl
2Saturated boric acid solution in, stir under 0-4 ℃, and be incubated 26 hours, filter out microballoon, and get final product with the physiological saline washing.
After testing, the coefficient of elasticity of microballoon is 98 N/m, and penetration time is 35 minutes, and percentage of damage is 4.4%.
Claims (3)
1. the preparation method of immobilization oil degradation bacterium is characterized in that comprising:
With PVA fully dissolving under 80-100 ℃, make 10% solution, then mix with 1% SA solution, add again the gac mixing, be cooled to 40 ℃ after, add the bacterium liquid that is in logarithmic phase, then splash into syringe and contain 3% CaCl
2Saturated boric acid solution in, stir under 0-4 ℃, and be incubated 26 hours, filter out microballoon, and get final product with the physiological saline washing.
2. the preparation method of immobilization oil degradation bacterium as claimed in claim 1, the add-on that it is characterized in that bacterium liquid is 20%.
3. the preparation method of immobilization oil degradation bacterium as claimed in claim 2, the add-on that it is characterized in that gac is 50 g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012105509729A CN103074323A (en) | 2012-12-18 | 2012-12-18 | Preparation method of immobilized crude oil degrading bacteria |
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| CN2012105509729A CN103074323A (en) | 2012-12-18 | 2012-12-18 | Preparation method of immobilized crude oil degrading bacteria |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018458A (en) * | 2015-07-31 | 2015-11-04 | 湖南大学 | Compound microbial agent as well as preparation method and application thereof |
| CN105087538A (en) * | 2015-07-29 | 2015-11-25 | 浙江大学 | Petroleum degrading bacterium as well as preparation method and application thereof |
| CN106076214A (en) * | 2016-07-15 | 2016-11-09 | 沈阳化工大学 | A kind of calcium alginate microsphere preparation method with nucleocapsid structure |
| CN109402105A (en) * | 2018-10-15 | 2019-03-01 | 山东交通学院 | A kind of microbe carrier that floatability is degradable and preparation method and application |
| CN110964711A (en) * | 2018-09-28 | 2020-04-07 | 天津大学 | Acid-resistant microbial capsule embedding liquid, microbial capsule, and preparation methods and applications of microbial capsule embedding liquid and microbial capsule |
| CN112122330A (en) * | 2020-08-29 | 2020-12-25 | 山西大学 | Microcapsule repairing agent for composite contaminated soil of coking field, preparation method and application |
-
2012
- 2012-12-18 CN CN2012105509729A patent/CN103074323A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087538A (en) * | 2015-07-29 | 2015-11-25 | 浙江大学 | Petroleum degrading bacterium as well as preparation method and application thereof |
| CN105087538B (en) * | 2015-07-29 | 2018-05-29 | 浙江大学 | A kind of oil degradation microbial inoculum and its preparation method and application |
| CN105018458A (en) * | 2015-07-31 | 2015-11-04 | 湖南大学 | Compound microbial agent as well as preparation method and application thereof |
| CN106076214A (en) * | 2016-07-15 | 2016-11-09 | 沈阳化工大学 | A kind of calcium alginate microsphere preparation method with nucleocapsid structure |
| CN106076214B (en) * | 2016-07-15 | 2019-12-17 | 沈阳化工大学 | preparation method of calcium alginate microspheres with core-shell structure |
| CN110964711A (en) * | 2018-09-28 | 2020-04-07 | 天津大学 | Acid-resistant microbial capsule embedding liquid, microbial capsule, and preparation methods and applications of microbial capsule embedding liquid and microbial capsule |
| CN110964711B (en) * | 2018-09-28 | 2024-03-22 | 天津大学 | Acid-resistant microbial capsule embedding liquid, microbial capsule, and preparation methods and applications thereof |
| CN109402105A (en) * | 2018-10-15 | 2019-03-01 | 山东交通学院 | A kind of microbe carrier that floatability is degradable and preparation method and application |
| CN112122330A (en) * | 2020-08-29 | 2020-12-25 | 山西大学 | Microcapsule repairing agent for composite contaminated soil of coking field, preparation method and application |
| CN112122330B (en) * | 2020-08-29 | 2022-05-27 | 山西大学 | Microcapsule repairing agent for composite contaminated soil of coking field, preparation method and application |
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Application publication date: 20130501 |