CN103070311A - Production method of eggs rich in lutein - Google Patents
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- CN103070311A CN103070311A CN2013100096885A CN201310009688A CN103070311A CN 103070311 A CN103070311 A CN 103070311A CN 2013100096885 A CN2013100096885 A CN 2013100096885A CN 201310009688 A CN201310009688 A CN 201310009688A CN 103070311 A CN103070311 A CN 103070311A
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Abstract
The invention discloses a production method of eggs rich in lutein, which can improve the stability of lutein, improve the production performance and immune function of layers as well as quality of egg products. The production method is characterized by comprising the following steps: a, during an egg laying period of the layers, feeding the layers with basic feed and an enzymic preparation, wherein the basic feed comprises corn, soybean meal, corn gluten meal for feedstuffs, rice bran meal, corn DDGS (distillers dried grains with soluble), wheat bran, stone powder, calcium hydrophosphate, salt, methionine, a vitamin premix, marigold pollen, a microelement premix, choline chloride, Bio-Aid, adsorption type vitamin E powder and compound betaine 903, and the enzymic preparation comprises alpha-amylase, xylanase, protease, mannase, pectinase and cellulose; and b, collecting eggs laid by the layers in two weeks.
Description
Technical field
The present invention relates to a kind of production method of egg, especially a kind of stability that improves lutein, the production method that is rich in lutein composition egg of performance in layers, immunity function and egg quality improved.
Background technology
Along with improving constantly of expanding economy and living standards of the people, the nutrient health of food and safety become the focus of consumer's growing interest, organic food, pollution-free food, healthy food, dietary supplements etc. are paid much attention in countries in the world, more particularly take bread and cheese and beverage as carrier, add the research and development of the functional food with disease resistance function factor, become both at home and abroad the competitively focus of exploitation.And the research of the functional components of plant origin has become the hot topic of the research fields such as food, feed, medicine, and people attempt to seek physiological activator from each Plants, in order to extenuate and to solve the problems such as ever-increasing various diseases.Lutein
BeA kind of natural yellow phytochrome extensively is present in vegetables, flowers, fruit and some algae bio, and its chemical constitution is the containing oxygen derivative that a class has the conjugated polyene hydrocarbon structure, and molecular formula is C
40H
56O
2, contain a plurality of conjugated double bonds in the structure.Its unique chemical constitution has determined that lutein has strikingly color and special biological function, studies confirm that, lutein plays important physiological action at aspects such as eyeshield, anti-oxidant, anticancer, angiocardiopathy preventing, enhancing immunity.U.S. FDA has just been ratified lutein and has been used for food as food supplement as far back as 1995, to improve nutritive value.U.S. Ross Product in 2000, Sunsweet Growers, Country Pure Food three companies have developed the eye health beverage that contains lutein.Switzerland Roche Holding Ag has released lutein and multivitamin formulated eyeshield chewable tablet and eyeshield vinosity glycocoll.The countries such as America and Europe have classified lutein as food additives, use at cake, candy, condiment, pickles.The international survey data shows that lutein and lutein ester are in very great demand on market, and tablet, hard shell capsules, soft capsule are arranged.
In recent years, the exploitation of domestic lutein health product has caused widely attention at food and pharmaceuticals industry.From present domestic disclosed 158 patent documentations relevant with lutein, most of patent concentrates on extraction process and the product stabilisation aspect of lutein, wherein has 46 patents to relate to the food relevant with lutein, drink, health products aspect.A kind of health care preparation and preparation method who is rich in lutein and beta carotene disclosed such as CN200810054657.0; CN200410079535.9 discloses a kind of formulation product that contains lutein and/or carotene linolenic acid and preparation method thereof; CN200510095096.3, CN200710200326.9 disclose respectively a kind of lutein complex capsule, xanthophyll soft capsule and preparation technology's thereof method; CN200410102869.3 discloses a kind of processing method of nutritious noodle of xanthin; CN201110413648.8 discloses a kind of Lutein xylitol nutritious drink and preparation method thereof; CN200810061382.3 discloses a kind of breeding method of tea with high lutein content and eye-benefiting function; CN200710142949.5 discloses the production and processing method of a kind of lutein drink, xanthophyll milk; CN201010208565.0 discloses a kind of baby milk powder that contains lutein and preparation method thereof; CN200810064421.5 discloses a kind of dietary supplements and preparation technology who contains natural carotenol; CN201019000006.8 discloses multi-dimensional xanthophyll preparation of a kind of powerfully protecting eyesight etc.Contain lutein food, drink, the health product overwhelming majority and only relate to that technique is relatively simple directly adds, the security (solvent and heavy-metal residual) that the adding procedure Lutein adds raw produce; raw products directly has influence on the security of final products, capsule and dietary supplements series products more near " medicine " thus limited the acceptability of these products.
Egg always is requisite important foodstuffs on consumer's dining table, also is human optimal nutraceutical, is described as " spirit of life ".The egg nutrient balanced composition is rich in the needed by human nutrients such as protein, phosphatide, VA, VB, VB2, VD, calcium, iron, lysozyme, has almost comprised whole nutrients of hatching life.Therefore be known as " the desirable nutrition library " of human body, often be used to estimate the standard of other animal food, simultaneously a large amount of studies confirm that, utilize egg nutrient to enrich especially the characteristics of balance, with it as carrier, medicine, functionalization nutrient that some is special are implanted in the egg by certain technology, thereby have become the important directions in functional food research and development field by the research that some functional nutrient composition of egg enrichment makes egg possess alimentary health-care function.
Lutein is the important antioxidants of a class, and its stability and activity in feed is the important guarantee that the egg Lutein can stably depositing.At present domestic still resting on for the application great majority of lutein in layer diets improved aspect yolk color and the yellow-feather broiler skin color; and by egg enrichment lutein; the lutein health-care eggs is rich in production; the research report that rarely has at home this respect; from 9 pieces of lutein documents relevant with laying hen finding; research concentrates on the document that lutein deposits and has 4 pieces in egg; as reporting yellow 2007 late autumn; by in layer diets, adding the content that the homemade lutein ester in laboratory can increase the egg Lutein; can make the lutein content in the egg reach 1.91mg/60 gram egg; but the homemade lutein ester additive in laboratory is owing to be not large-scale production; the uniformity that the stability of product reaches effect in laying hen produces is not confirmed; and in process of the test, do not consider cost and the market factor; the optimum addition and the deposition that do not provide the layer diets Lutein of producing the lutein egg reach the stable time, do not provide the recommended intake and the edible ratio that is rich in the satisfied day recommended intake of lutein egg that are fit to healthy population in the paper.
Unit's Na etc. was studied and has been added Flos Tagetis Erectae extract and vitamin E in the layer diets to the impact of egg quality, anti-oxidant energy and immune response in 2009, the result shows: Flos Tagetis Erectae extract and vitamin E are to the quality of egg, the improvement of shell thickness, raw egg yolk color, ripe egg yolk color value, lutein transformation efficiency, GSH-Px in serum activity and blood lipids index exist makes effect mutually.Though provided the recommendation addition in the literary composition, but recommended intake and the edible ratio that is rich in the satisfied day recommended intake of lutein egg with suitable healthy population do not connect, do not provide addition and the stabilization technology of natural carotenol product in the layer diets of the production lutein egg that adapts with market sale, also not mentionedly reach optimal deposition amount required time in the egg yet.
Summary of the invention
The present invention is in order to solve the existing above-mentioned technical problem of prior art, a kind of stability that improves lutein is provided, improves the production method that is rich in lutein composition egg of performance in layers, immunity function and egg quality.
Technical solution of the present invention is: a kind of production method that is rich in lutein composition egg is characterized in that carrying out as follows successively:
A. during Egg Production of Laying Hens, the following basic feed of the laying hen of feeding and enzyme preparation;
Basic feed ingredient is by quality ratio: corn 500 ~ 650, dregs of beans 150 ~ 240, feed be with corn protein powder 15 ~ 20, rice bran meal 20 ~ 30, corn DDGS 50 ~ 100, wheat bran 20 ~ 30, stone flour 65 ~ 70, calcium monohydrogen phosphate 5 ~ 15, salt 2 ~ 4, methionine 0.8 ~ 1, vitamin premix 0.3 ~ 0.5, natural carotenol feed addictive or marigold pollen 1.0 ~ 10.0, microelement pre-mix material 1.0 ~ 2.0, Choline Chloride 1.0 ~ 2.0, the happy Bio-Aid:0.5 of beneficial bacterium ~ 1.5, absorbent-type vitamin E powder: 0.5 ~ 1.0 and compound betaine 903:0.2 ~ 0.4;
The scale of feeding of described enzyme preparation is take the basic feed of per kilogram as reference, and concrete consumption is as follows: AMS: 75U/kg ~ 105U/kg, zytase: 3500U/kg ~ 4000U/kg, protease: 2500U/kg ~ 3500U/kg, mannase: 125U/kg ~ 175U/kg, pectase: 125U/kg ~ 175U/kg, cellulase: 1625U/kg ~ 2275U/kg, dextranase: 500U/kg ~ 700U/kg, micropill 5000 phytases: 300U/kg ~ 500U/kg;
B. after two weeks, collect the egg that laying hen produces.
The present invention adds the natural carotenol feed addictive in the mixed feed of laying cycle of laying hens, the Multiple components such as probiotics, fodder enzyme preparation, various component interactions, by the laying hen bio-transformation, can improve the stability of egg Lutein, make to be rich in the egg to reach more than the lutein 15mg/kg, the edible 2 pieces of lutein eggs of per day for adults can satisfy the requirement more than 30% of adult meals day recommended intake 6mg; Simultaneously can guarantee egg quality, increase yolk color (bringing up to 9 ~ 11 by Luo Shi colorimetric fan gamut of chromaticities 6 ~ 7), and can improve laying hen health status, improve performance in layers and immunity function (as improving antibody titer of ND).
The specific embodiment
Embodiment 1:
A. during Egg Production of Laying Hens, the following basic feed of the laying hen of feeding and enzyme preparation;
Basic feed ingredient is in kilogram: corn 600, dregs of beans 200, feed be with corn protein powder 18, rice bran meal 25, corn DDGS 75, wheat bran 25, stone flour 68, calcium monohydrogen phosphate 10, salt 3, methionine 0.9, vitamin premix 0.4, natural carotenol feed addictive 5.0, microelement pre-mix material 1.5, Choline Chloride 1.5, the happy Bio-Aid:1.0 of beneficial bacterium, absorbent-type vitamin E powder: 0.8 and compound betaine 903:0.3;
The scale of feeding of described enzyme preparation is take the basic feed of per kilogram as reference, and concrete consumption is as follows: AMS: 80U/kg, zytase: 3800U/kg, protease: 3000U/kg, mannase: 150U/kg, pectase: 150U/kg, cellulase: 2000U/kg, dextranase: 600U/kg, micropill 5000 phytases: 400U/kg;
B. after two weeks, collect the egg that laying hen produces.
Each raw material sources is as follows:
| Material name | Sale enterprise |
| Corn | The Dalian standing grain rises grain and oil economy and trade Co., Ltd |
| Dregs of beans | 93 Dalian soybean Science and Technology Ltd.s of group |
| The feed corn protein powder | Starch sugar Co., Ltd is kindly helped secure the success of in Changchun |
| Rice bran meal | The prosperous feed corporation,Ltd that reaches in Dalian |
| Corn DDGS | Meihekou City Fukang alcohol Co., Ltd |
| Wheat bran (high-quality) | Yantai platform China food Industrial Co., Ltd. |
| Stone flour | Pian Ling town, Xiuyan sincere stone flour factory |
| Calcium monohydrogen phosphate | Yunnan Xinlong Mineral Animal Feed Co., Ltd. |
| The natural carotenol feed addictive | Lidaer Biotech Co., Ltd., Guangzhou |
| Salt | Dalian Lvshunkou District salt industry Co., Ltd |
| Methionine | Evonik Degussa (China) Co., Ltd. |
| Vitamin premix | Shen Yang Boin Feed Co., Ltd.'s (product: universal vitamin premix 717) |
| The absorbent-type vitamin E powder | DSM vitamin (Shanghai) Co., Ltd. |
| Microelement pre-mix material | The earth mineral substance premix factory of Anshan Qianshan District Xingda |
| Choline Chloride | Believe bio tech ltd in Liaoning |
| The happy Bio-Aid of benefit bacterium | Beijing China industrial Science and Technology Ltd. of occasion |
| AMS, zytase, protease, mannonase pectase, cellulase, dextranase | Beijing Ke Weibo bio tech ltd |
| Micropill 5000 phytases | Sunrise ocean, Beijing development in science and technology Co., Ltd |
| Compound betaine 903 | Sunrise ocean, Beijing development in science and technology Co., Ltd |
Embodiment 2:
A. during Egg Production of Laying Hens, the following basic feed of the laying hen of feeding and enzyme preparation;
Basic feed ingredient is in kilogram: corn 500, dregs of beans 150, feed be with corn protein powder 15, rice bran meal 20, corn DDGS 50, wheat bran 20, stone flour 65, calcium monohydrogen phosphate 5, salt 2, methionine 0.8, vitamin premix 0.3, natural carotenol feed addictive 1.0, microelement pre-mix material 1.0, Choline Chloride 1.0, the happy Bio-Aid:0.5 of beneficial bacterium, absorbent-type vitamin E powder: 0.5 and compound betaine 903:0.2;
The scale of feeding of described enzyme preparation is take the basic feed of per kilogram as reference, and concrete consumption is as follows: AMS: 75U/kg, zytase: 3500U/kg, protease: 2500U/kg, mannase: 125U/kg, pectase: 125U/kg, cellulase: 1625U/kg, dextranase: 500U/kg, micropill 5000 phytases: 300U/kg;
B. after two weeks, collect the egg that laying hen produces.
Raw material sources are with embodiment 1.
Embodiment 3:
A. during Egg Production of Laying Hens, the following basic feed of the laying hen of feeding and enzyme preparation;
Basic feed ingredient is in kilogram: corn 650, dregs of beans 240, feed be with corn protein powder 20, rice bran meal 30, corn DDGS 100, wheat bran 30, stone flour 70, calcium monohydrogen phosphate 15, salt 4, methionine 1, vitamin premix 0.5, marigold pollen 10.0, microelement pre-mix material 2.0, Choline Chloride 2.0, the happy Bio-Aid:1.5 of beneficial bacterium, absorbent-type vitamin E powder: 1.0 and compound betaine 903:0.4;
The scale of feeding of described enzyme preparation is take the basic feed of per kilogram as reference, and concrete consumption is as follows: AMS: 105U/kg, zytase: 4000U/kg, protease: 3500U/kg, mannase: 175U/kg, pectase: 175U/kg, cellulase: 2275U/kg, dextranase: 700U/kg, micropill 5000 phytases: 500U/kg;
B. after two weeks, collect the egg that laying hen produces.
Each raw material sources is except marigold pollen, and is all identical with embodiment 1.
The preparation method of marigold pollen be the marigold flower that will gather dry or 40 ~ 60 ℃ dry to water content and reach 8 ~ 10%, be crushed to 20 ~ 80 order granularities.
Test and result:
1. experimental design scheme:
Adopt control design.
Choose laying rate, body weight is close, 360 of the healthy blue brown shell laying hens in 28 seas in age in week, be divided at random 2 groups, one group is control group, and another group is test group, establish 6 repetitions for every group, each repeats 30 chickens, and conventional layer diets is fed in the group feeding of control group chicken, the test group embodiment of the invention 1 of feeding, carry out check experiment, test and measure index of correlation after 14 days, 21 days, 28 days.
2. testing index:
Nutritive index: lutein content;
Production performance index: laying rate, a daily output, feedstuff-egg ratio, enter to give up the death rate;
Immune indexes: ND antibody titer;
Egg quality index: shell thickness, Hough unit.
3. result:
3.1 lutein index
The lutein index sees Table 1.
Table 1
Annotate: foreign literature report (piece heavily calculating as 60 grams take egg): the common egg of Finland: 2mg/kg; The common egg in Scotland: 4.17mg/kg; The common egg of the U.S.: 4.87mg/kg.
3.2 production performance index and immune function
Production performance index and immune function see Table 2
Table 2
| Index | Control group | Test group | Test group contrast group |
| Laying rate (%) | 78.81±5.27 a | 80.35±5.49 b | 1.54 |
| The daily output (gram) | 49.80±1.70 a | 50.88±2.16 b | 1.08 |
| Feedstuff-egg ratio | 2.34±.002 a | 2.29±.001 b | -0.05 |
| Enter to give up the death rate (%) | 2.36 | 1.55 | -0.81 |
| The ND antibody titer | 9.0±2.70 a | 10.3±1.05 b | 1.3 |
3.3 egg quality index
The egg quality index sees Table 3
Table 3
| Index | Control group | Test group | Test group contrast group |
| Shell thickness (mm) | 0.341±0.007 | 0.356±0.006 | 0.015 |
| Hough unit | 78.21±1.69 | 80.66±1.51 | 2.45 |
Annotate: Hough unit is the important indicator of weighing egg freshness by using, and relevant with albumen height and egg size, the Hough unit value is higher, and egg freshness by using is higher.
The result shows that simultaneously the present invention can improve laying hen health status, improves performance in layers, immunity function and egg quality.
Claims (1)
1. production method that is rich in lutein composition egg is characterized in that carrying out as follows successively:
A. during Egg Production of Laying Hens, the following basic feed of the laying hen of feeding and enzyme preparation;
Basic feed ingredient is by quality ratio: corn 500 ~ 650, dregs of beans 150 ~ 240, feed be with corn protein powder 15 ~ 20, rice bran meal 20 ~ 30, corn DDGS 50 ~ 100, wheat bran 20 ~ 30, stone flour 65 ~ 70, calcium monohydrogen phosphate 5 ~ 15, salt 2 ~ 4, methionine 0.8 ~ 1, vitamin premix 0.3 ~ 0.5, natural carotenol feed addictive or marigold pollen 1.0 ~ 10.0, microelement pre-mix material 1.0 ~ 2.0, Choline Chloride 1.0 ~ 2.0, the happy Bio-Aid:0.5 of beneficial bacterium ~ 1.5, absorbent-type vitamin E powder: 0.5 ~ 1.0 and compound betaine 903:0.2 ~ 0.4;
The scale of feeding of described enzyme preparation is take the basic feed of per kilogram as reference, and concrete consumption is as follows: AMS: 75U/kg ~ 105U/kg, zytase: 3500U/kg ~ 4000U/kg, protease: 2500U/kg ~ 3500U/kg, mannase: 125U/kg ~ 175U/kg, pectase: 125U/kg ~ 175U/kg, cellulase: 1625U/kg ~ 2275U/kg, dextranase: 500U/kg ~ 700U/kg, micropill 5000 phytases: 300U/kg ~ 500U/kg;
B. after two weeks, collect the egg that laying hen produces.
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| CN104431513A (en) * | 2014-12-05 | 2015-03-25 | 熙悦山农业科技(北京)有限公司 | Feed for hens laying lutein-containing eggs |
| CN104621414A (en) * | 2015-03-16 | 2015-05-20 | 邵阳学院 | Egg-chicken feed additive for producing xanthophyll-enriched eggs by using orange peel and preparation method of egg-chicken feed additive |
| CN105230971A (en) * | 2015-11-15 | 2016-01-13 | 邵阳学院 | Method for producing feed additive for laying hens with waste fruit peels and fruit kernels |
| CN105941997A (en) * | 2016-06-15 | 2016-09-21 | 环山集团有限公司 | Pure barley laying hen formula feed and production method |
| CN106538871A (en) * | 2016-10-20 | 2017-03-29 | 镇远县蕉溪镇蛋鸡养殖农民专业合作社 | A kind of egg feedstuff for improving egg lutein content and preparation method thereof |
| EP3150257A1 (en) * | 2015-09-30 | 2017-04-05 | Buga, Doris | A method for producing a composition for strengthening the immune system |
| CN107080095A (en) * | 2017-05-17 | 2017-08-22 | 广西品位生物科技有限公司 | A kind of biological chicken feed |
| CN107518202A (en) * | 2016-06-21 | 2017-12-29 | 孙洪军 | A kind of bird feed additive |
| CN108812538A (en) * | 2018-06-20 | 2018-11-16 | 昆明藻能生物科技有限公司 | A kind of preparation method of the nutritional egg with sight protectio and enhancing |
| CN110169493A (en) * | 2019-07-08 | 2019-08-27 | 天津市畜牧兽医研究所 | A kind of feed for increasing yolk color grade and improving laying rate of laying hen |
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| CN104431513A (en) * | 2014-12-05 | 2015-03-25 | 熙悦山农业科技(北京)有限公司 | Feed for hens laying lutein-containing eggs |
| CN104621414A (en) * | 2015-03-16 | 2015-05-20 | 邵阳学院 | Egg-chicken feed additive for producing xanthophyll-enriched eggs by using orange peel and preparation method of egg-chicken feed additive |
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| CN105941997A (en) * | 2016-06-15 | 2016-09-21 | 环山集团有限公司 | Pure barley laying hen formula feed and production method |
| CN107518202A (en) * | 2016-06-21 | 2017-12-29 | 孙洪军 | A kind of bird feed additive |
| CN106538871A (en) * | 2016-10-20 | 2017-03-29 | 镇远县蕉溪镇蛋鸡养殖农民专业合作社 | A kind of egg feedstuff for improving egg lutein content and preparation method thereof |
| CN107080095A (en) * | 2017-05-17 | 2017-08-22 | 广西品位生物科技有限公司 | A kind of biological chicken feed |
| CN108812538A (en) * | 2018-06-20 | 2018-11-16 | 昆明藻能生物科技有限公司 | A kind of preparation method of the nutritional egg with sight protectio and enhancing |
| CN110169493A (en) * | 2019-07-08 | 2019-08-27 | 天津市畜牧兽医研究所 | A kind of feed for increasing yolk color grade and improving laying rate of laying hen |
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Application publication date: 20130501 |
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