CN103063751A - Detection method for determining polymer impurities in mezlocillin sodium - Google Patents
Detection method for determining polymer impurities in mezlocillin sodium Download PDFInfo
- Publication number
- CN103063751A CN103063751A CN2011103200650A CN201110320065A CN103063751A CN 103063751 A CN103063751 A CN 103063751A CN 2011103200650 A CN2011103200650 A CN 2011103200650A CN 201110320065 A CN201110320065 A CN 201110320065A CN 103063751 A CN103063751 A CN 103063751A
- Authority
- CN
- China
- Prior art keywords
- detection method
- mezlocillin sodium
- detection
- chromatographic column
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a detection method for determining polymer impurities in mezlocillin sodium. The detection method employs a high performance liquid chromatography size exclusion chromatography. The chromatography conditions are as follows: a chromatographic column employs hydrophilic silica for globular protein as a stationary phase and 50 mmol of a phosphate buffered solution as a mobile phase; a flow velocity of the mobile phase is 0.5-2.0 mL/min; a detection wavelength is 230-254 nm; a column temperature is a room temperature; a sample volume is 10-80 [mu]L; granularity of the chromatographic column stationary phase is 5 [mu]m, aperture of the chromatographic column stationary phase is 10 nm; the inner diameter of the chromatographic column is 7.8 mm; and the column length is 300 mm. he detection method can perform rapidly qualitative and quantitative determination on the polymer impurities in mezlocillin sodium, simultaneously simplifies operation steps, increases detection precision and shortens detection time.
Description
Technical field
The present invention relates to the volume exclusion detection field, more specifically, relate to a kind of detection method of measuring polymeric impurities in the mezlocillin sodium.
Background technology
Mezlocillin sodium is a kind of semi-synthetic penicillins microbiotic, and the gram positive bacterias such as Escherichia coli, Enterobacter, proteus are had stronger antibacterial action.The mezlocillin is a kind of acylureido-penicilin derivant through the enteron aisle external administration, and bactericidal action is arranged when heavy dose of.The antimicrobial spectrum of the mezlocillin sodium basically antimicrobial spectrum with ampicillin and carbenicillin is identical.But in mezlocillin sodium, also have some macromolecule allergic dopants.Therefore, in the quality standard of this medicine, need to control the total amount of macromolecule impurity.
At present, 2010 editions " Chinese pharmacopoeia adopts the mesolow liquid phase chromatography detection method for the detection of the polymeric impurities in the mezlocillin sodium, and adopts Sephadex G-10 to be fixing phase.When adopting traditional glass gel chromatographic columns (Sephadex G-10) to separate in the mezlocillin sodium polymeric impurities, the post effect is lower, and general post effect only is 400 ~ 1000; Simultaneously, such chromatographic column in actual use complex operation, disengaging time is long, degree of separation is poor, brings inconvenience for the accurate qualitative and quantitative test of impurity.
Therefore, be necessary to provide a kind of detection method of measuring polymeric impurities in the mezlocillin sodium.
Summary of the invention
The object of the invention is to propose a kind of detection method of measuring polymeric impurities in the mezlocillin sodium, it can be made fast qualitative to the polymeric impurities in the mezlocillin sodium and quantitatively detect.
For achieving the above object, the invention provides a kind of detection method of measuring polymeric impurities in the mezlocillin sodium, described detection method adopts high performance liquid chromatography size exclusion chromatograph method, and it comprises:
The mezlocillin sodium sample dissolution is obtained sample solution; And
Employing take globular preteins with hydrophilic silica gel as fixing phase, take phosphate buffered solution as mobile phase, flow rate of mobile phase is 0.5-2.0mL/min, column temperature is 20-35 ℃, adopts UV-detector that sample solution is carried out liquid chromatographic detection.
As a further improvement on the present invention, the detection wavelength coverage of described UV-detector is 230nm-254nm.
As a further improvement on the present invention, the detection wavelength of described UV-detector is 254nm.
As a further improvement on the present invention, the granularity of described chromatographic column fixed phase is 5 microns, and the aperture is 100, and the post interior diameter of described chromatographic column is 7.8mm, and length is 300mm in the post.
As a further improvement on the present invention, the pH value scope of described buffer solution is 7.0 ~ 8.0.
As a further improvement on the present invention, described detection method also comprises: the mezlocillin sodium sample dissolution in water, is filtered take the aperture as the miillpore filter of 0.45um or 0.22um described sample solution.
As a further improvement on the present invention, described globular preteins is 1000-10000 with the molecular weight usable range of hydrophilic silica gel.
As a further improvement on the present invention, mezlocillin sodium is less than or equal to 11 minutes with the time that polymeric impurities is wherein separated fully.
As a further improvement on the present invention, the degree of separation of described detection method is more than or equal to 4.5, and tailing factor is less than or equal to 1.5.
Compared with prior art, a kind of detection method of measuring polymeric impurities in the mezlocillin sodium provided by the invention, it is simple to operate, and disengaging time shortens, and degree of separation is high, can be fast to the accurate fast qualitative quantitative test of the polymeric impurities in the mezlocillin sodium.
Description of drawings
Accompanying drawing is used to provide a further understanding of the present invention, is used from explanation the present invention with various embodiments of the present invention one, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the chromatogram of mezlocillin sodium and wherein polymeric impurities in the embodiment of the invention 1;
Fig. 2 is the detection data plot in the chromatogram shown in Figure 1;
Fig. 3 is the chromatogram of mezlocillin sodium and wherein polymeric impurities in the embodiment of the invention 2;
Fig. 4 is the detection data plot in the chromatogram shown in Figure 3;
Fig. 5 is the chromatogram of mezlocillin sodium and wherein polymeric impurities in the embodiment of the invention 3;
Fig. 6 is the detection data plot in the chromatogram shown in Figure 5;
Fig. 7 is the chromatogram of mezlocillin sodium and wherein polymeric impurities in the embodiment of the invention 4;
Fig. 8 is the detection data plot in the chromatogram shown in Figure 7;
Fig. 9 is the present invention and existing 2010 editions " the comparing result figure of Chinese pharmacopoeia detection method.
Embodiment
Accompanying drawing is used to provide a further understanding of the present invention, is used from explanation the present invention with various embodiments of the present invention one, but is not construed as limiting the invention.The invention paper term definition and explanation, then introduced a kind of related embodiment of measuring the high efficiency liquid phase volume exclusion detection method of polymeric impurities in the mezlocillin sodium.
1. definition and the explanation of used term in instructions and the claim
Before introducing in detail following embodiment details, define first or set forth some terms.
Term " post effect " refers to the separation efficiency for the quantificational expression chromatographic column, and it is weighed with " theoretical cam curve ".
Term " Plates " refers to theoretical cam curve, theoretical cam curve (theoretical plate number), and one of post effect parameter of N chromatogram is for the separation efficiency (being called for short the post effect) of quantificational expression chromatographic column.N depends on that the kind of kind, character (granularity, particle diameter distribution etc.), filling situation, column length, mobile phase of fixing phase and flow velocity and measuring column imitate the character of used material.The N value is larger, illustrates that the performance of chromatographic column is better, the detection of used more suitable this sample of mobile phase.
Miillpore filter: be divided into water film and organic phase film according to the purposes difference, Main Function is the insoluble impurities of removing in the sample, prevents that impurity particle from stopping up the instrument pipeline.
Sample introduction concentration and sample size: reacted the sensitivity of the chromatographic column of using and method, the sensitivity of the lower explanation chromatographic column of sample introduction concentration and sample size and using method is higher.
Term " degree of separation " refers to the ratio of difference and average peak width of the retention time at adjacent two peaks.Also be resolution, represent the separation degree at adjacent two peaks.R is larger, shows that the two adjacent groups separation is better.When R＜1, two peaks overlap in general; When R=1.0, degree of separation can reach 98%; When R=1.5, degree of separation can reach 99.7%.Usually divide the sign that separates fully as two adjacent groups with R=1.5.2010 editions " Chinese pharmacopoeia reaches 1.5 separation case with degree of separation and is called baseline separation, and R 〉=1.5 are called fully and separate.
Term " high performance liquid chromatography " refers to take liquid as mobile phase, adopt the high pressure transfusion system, the mobile phase such as mixed solvent, damping fluid that will have the single solvent of opposed polarity or different proportion pumps into the fixedly chromatographic column of phase is housed, in post each composition separated after, enter detecting device and detect, thereby realization is to the analysis of sample.
Term " RT " refers to retention time, it refers to that separated sample component begins to occur the time of this concentration of component maximum value from sample introduction behind post, also both became civilized the time that the temple experiences till during to fixed point that certain component chromatographic peak occurs from sample introduction, the retention time that is called this component, represent with RT, often take minute (min) as chronomere.
Term " Tailing " refers to that tailing factor, tailing factor are the parameters of recently estimating peak shape by the distance of calculating 5% peak height place peak width and peak maximum to forward position, and purpose is in order to guarantee chromatographic resolution effect and measuring accuracy, and T commonly used represents.T illustrates that more near 1 the symmetry at peak is better.
In the embodiment explanation, symbol g=gram, the mL=milliliter, the mol=mole, ℃=degree centigrade, mmol=mM, um=micron (1 meter=1 * 10
6Micron), nm=nanometer (1 meter=1 * 10
9Nanometer) ,=dust (1 meter=1 * 10
10), uL=microlitre (1 liter=1 * 10
6Microlitre).
The invention provides a kind of detection method of measuring polymeric impurities in the mezlocillin sodium, it comprises: the mezlocillin sodium sample dissolution is obtained sample solution; The concentration of described sample solution is 5-10mg/mL, and described sample solution is filtered take the aperture as the miillpore filter of 0.45um or 0.22um.And adopt take globular preteins with hydrophilic silica gel as fixing phase, take phosphate buffered solution as mobile phase, the pH value scope of described buffer solution is 7.0 ~ 8.0, and flow rate of mobile phase is 0.5-2.0mL/min, column temperature is 20-35 ℃, adopts UV-detector that sample solution is carried out liquid chromatographic detection.
Wherein, the detection wavelength coverage of described UV-detector is 230nm-254nm.
Wherein, the granularity of described chromatographic column fixed phase is 5 microns, and the aperture is 100, and the post interior diameter of described chromatographic column is 7.8mm, and length is 300mm in the post.
Can carry out fast qualitative to the polymeric impurities in the mezlocillin sodium according to above-mentioned detection method detects.Below with a plurality of embodiment the detection method of polymeric impurities in the mensuration mezlocillin sodium of the present invention is described.
Embodiment 1
It is an amount of at first to take by weighing the mezlocillin sodium sample, and water dissolves fully, and making concentration is the detected sample solution of 10mg/L.This sample solution is filtered and for subsequent use take the aperture as the miillpore filter of 0.45um.
Adopting SEPAX company model is the liquid-phase chromatographic column of the size exclusion chromatograph filling agent filling of SRT-C, and the granularity of this filling agent is 5 microns, and the aperture is 100.The diameter of this chromatographic column is 7.8mm, and length is 300mm.
The chromatographic condition that adopts is: take the phosphate buffered solution of 50mmol as mobile phase, the pH value is 8.0; Flow velocity 0.5mL/min; The detection wavelength of UV-detector is 254nm; Sample size is 20uL; Sample introduction concentration is 10mg/mL; Column temperature is 20 ℃.
According to above-mentioned chromatographic condition, carry out high performance liquid chromatography and detect testing result such as Fig. 1 and shown in Figure 2.In conjunction with Fig. 1 and Fig. 2, we find to contain in the mezlocillin sodium three kinds of impurity, and it reached maximum at 8.493 minutes, 8.702 minutes with 8.911 minutes peak concentration.The peak concentration of mezlocillin sodium in the time of 10.225 minutes reaches maximum simultaneously, and in 11 minutes, the concentration of mezlocillin sodium trend zero.This explanation target component mezlocillin sodium this moment is separated fully.This testing result shows that the total amount of polymeric impurities in this mezlocillin sodium sample is that 3.61%(calculates by area percentage), the degree of separation of impurity and main peak is 6.03, be 20239 by the mezlocillin sodium theory of computation number of plates, the tailing factor of mezlocillin sodium is 1.00.
Embodiment 2
It is an amount of at first to take by weighing the mezlocillin sodium sample, and water dissolves fully, and making concentration is the detected sample solution of 10mg/L.This sample solution is filtered and for subsequent use take the aperture as the miillpore filter of 0.45um.
Adopting SEPAX company model is the liquid-phase chromatographic column of the size exclusion chromatograph filling agent filling of SRT-C, and the granularity of this filling agent is 5 microns, and the aperture is 100.The post interior diameter of this chromatographic column is 7.8mm, and length is 300mm in the post.
The chromatographic condition that adopts is: take the phosphate buffered solution of 25mmol as mobile phase, the pH value is 7.0; Flow velocity 1.0mL/min; UV detection wavelength 230nm; Sample size: 10uL; Sample introduction concentration is 10mg/mL; Column temperature is 25 ℃.
According to above-mentioned chromatographic condition, to carry out high performance liquid chromatography and detect, testing result is as shown in Figure 3 and Figure 4.In conjunction with Fig. 3 and Fig. 4, we find to contain in the mezlocillin sodium two kinds of impurity, and it reached maximum at 8.533 minutes with 8.918 minutes peak concentration.The peak concentration of mezlocillin sodium in the time of 10.14 minutes reaches maximum simultaneously, and in 11 minutes, the concentration of mezlocillin sodium trend zero.This explanation target component mezlocillin sodium this moment is separated fully.This testing result shows that the total amount of polymeric impurities in this mezlocillin sodium material sample is that 2.89%(calculates by area percentage), the degree of separation of impurity and main peak is 4.84, be 12926 by the mezlocillin sodium theory of computation number of plates, the tailing factor of mezlocillin sodium is 1.10.
Embodiment 3
It is an amount of at first to take by weighing the mezlocillin sodium sample, and water dissolves fully, and making concentration is the detected sample solution of 5mg/L.This sample solution is filtered and for subsequent use take the aperture as the miillpore filter of 0.22um.
Adopting SEPAX company model is the liquid-phase chromatographic column of the size exclusion chromatograph filling agent filling of SRT-C, and the granularity of this filling agent is 5 microns, and the aperture is 100.The post interior diameter of this chromatographic column is 7.8mm, and length is 300mm in the post.
The chromatographic condition that adopts is: take the phosphate buffered solution of 50mmol as mobile phase, the pH value is 7.0; Flow velocity 1.0mL/min; UV detection wavelength 254nm; Sample size: 20uL; Sample introduction concentration is 5mg/mL; Column temperature is 30 ℃.
According to above-mentioned chromatographic condition, carry out high performance liquid chromatography and detect testing result such as Fig. 5 and shown in Figure 6.In conjunction with Fig. 5 and Fig. 6, we find to contain in the sodium sample of mezlocillin two kinds of impurity, and it reached maximum at 8.533 minutes with 8.924 minutes peak concentration.The peak concentration of mezlocillin sodium in the time of 10.170 minutes reaches maximum simultaneously, and in 11 minutes, the concentration of mezlocillin sodium trend zero.This explanation target component mezlocillin sodium this moment is separated fully.This testing result shows that the total amount of polymeric impurities in this mezlocillin sodium sample is that 2.64%(calculates by area percentage), the degree of separation of impurity and main peak is 5.28, is 15735 by the mezlocillin sodium theory of computation number of plates.The tailing factor of mezlocillin sodium is 1.12.
Embodiment 4
It is an amount of at first to take by weighing the mezlocillin sodium sample, and water dissolves fully, and making concentration is the detected sample solution of 5mg/L.Filter for subsequent use to this sample solution take the aperture as the miillpore filter of 0.22um.
Adopting SEPAX company model is the liquid-phase chromatographic column of the size exclusion chromatograph filling agent filling of SRT-C, and the granularity of this filling agent is 5 microns, and the aperture is 100.The post interior diameter of this chromatographic column is 7.8mm, and length is 300mm in the post.
The chromatographic condition that adopts is: take the phosphate buffered solution of 40mmol as mobile phase, the pH value is 7.0; Flow velocity 2.0mL/min; UV detection wavelength 254nm; Sample size: 80uL; Sample introduction concentration is 5mg/mL; Column temperature is 35 ℃.
According to above-mentioned chromatographic condition, carry out high performance liquid chromatography and detect testing result such as Fig. 7 and shown in Figure 8.In conjunction with Fig. 7 and Fig. 8, we find to contain in the mezlocillin sodium three kinds of impurity, and its peak concentration in the time of 8.53 minutes, 8.91 minutes and 9.20 minutes reaches maximum.The peak concentration of mezlocillin sodium in the time of 10.199 minutes reaches maximum simultaneously, and in 11 minutes, the concentration of mezlocillin sodium trend zero.This explanation target component mezlocillin sodium this moment is separated fully.This testing result shows that the total amount of polymeric impurities in this mezlocillin sodium sample is that 2.85%(calculates by area percentage), the degree of separation of impurity and main peak is 5.30, is 20747 by the mezlocillin sodium theory of computation number of plates.The tailing factor of mezlocillin sodium is 1.05.
As shown in Figure 9, we can find out, " for the technical scheme of the disclosed detection method of Chinese pharmacopoeia, sample size diminishes a kind of detection method of measuring polymeric impurities in the mezlocillin sodium disclosed by the invention with respect to 2010 editions, degree of separation improves, and tailing factor reduces.Illustrate that this detection method is simple to operate, disengaging time is short, and degree of separation is high, can be fast to the accurate fast qualitative analysis utilizing of the polymeric impurities in the mezlocillin sodium and measurement.
Be to be understood that, although this instructions is described according to embodiment, but be not that each embodiment only comprises an independently technical scheme, this narrating mode of instructions only is for clarity sake, those skilled in the art should make instructions as a whole, technical scheme among each embodiment also can through appropriate combination, form other embodiments that it will be appreciated by those skilled in the art that.
Above listed a series of detailed description only is specifying for feasibility embodiment of the present invention; they are not to limit protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill spirit of the present invention does and all should be included within protection scope of the present invention.
Claims (9)
1. detection method of measuring polymeric impurities in the mezlocillin sodium, described detection method adopt high performance liquid chromatography size exclusion chromatograph method, it is characterized in that, comprising:
The mezlocillin sodium sample dissolution is obtained sample solution; And
Employing take globular preteins with hydrophilic silica gel as fixing phase, take phosphate buffered solution as mobile phase, flow rate of mobile phase is 0.5-2.0mL/min, column temperature is 20-35 ℃, adopts UV-detector that sample solution is carried out liquid chromatographic detection.
2. detection method according to claim 1, it is characterized in that: the detection wavelength coverage of described UV-detector is 230nm-254nm.
3. detection method according to claim 2, it is characterized in that: the detection wavelength of described UV-detector is 254nm.
4. detection method according to claim 1, it is characterized in that: the granularity of described fixedly phase is 5 microns, and the aperture is 100, and the post interior diameter of described chromatographic column is 7.8mm, and length is 300mm in the post.
5. detection method according to claim 1, it is characterized in that: the pH value scope of described buffer solution is 7.0 ~ 8.0.
6. detection method according to claim 1 is characterized in that, described detection method also comprises: the mezlocillin sodium sample dissolution in water, is filtered take the aperture as the miillpore filter of 0.45um or 0.22um described sample solution.
7. detection method according to claim 1, it is characterized in that: described globular preteins is 1000-10000 with the molecular weight usable range of hydrophilic silica gel.
8. detection method according to claim 1, it is characterized in that: mezlocillin sodium is less than or equal to 11 minutes with the time that polymeric impurities is wherein separated fully.
9. detection method according to claim 1, it is characterized in that: the degree of separation of described detection method is more than or equal to 4.5, and tailing factor is less than or equal to 1.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103200650A CN103063751A (en) | 2011-10-20 | 2011-10-20 | Detection method for determining polymer impurities in mezlocillin sodium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103200650A CN103063751A (en) | 2011-10-20 | 2011-10-20 | Detection method for determining polymer impurities in mezlocillin sodium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103063751A true CN103063751A (en) | 2013-04-24 |
Family
ID=48106462
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103200650A Pending CN103063751A (en) | 2011-10-20 | 2011-10-20 | Detection method for determining polymer impurities in mezlocillin sodium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103063751A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018098502A1 (en) * | 2016-11-28 | 2018-05-31 | Board Of Regents, The University Of Texas System | Systems, methods and devices for width-based analysis of peak traces |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482544A (en) * | 2009-02-17 | 2009-07-15 | 南京工业大学 | Method for detecting cefuroxime sodium polymer impurities |
| CN101852782A (en) * | 2009-04-02 | 2010-10-06 | 鲁南制药集团股份有限公司 | Method for measuring impurity content of faropenem polymers in faropenem sodium raw materials and preparations |
-
2011
- 2011-10-20 CN CN2011103200650A patent/CN103063751A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482544A (en) * | 2009-02-17 | 2009-07-15 | 南京工业大学 | Method for detecting cefuroxime sodium polymer impurities |
| CN101852782A (en) * | 2009-04-02 | 2010-10-06 | 鲁南制药集团股份有限公司 | Method for measuring impurity content of faropenem polymers in faropenem sodium raw materials and preparations |
Non-Patent Citations (4)
| Title |
|---|
| 《中华人民共和国药典2010 年版(二部)》 20101001 国家药典委员会 美洛西林钠 中国医药科技出版社 , * |
| 周丽君，陈摇亮，滕红菊，罗摇艳，曹于平: "高效液相色谱法测定注射用头孢地嗪钠有关物质(聚合物)及其方法学研究", 《安徽医药》 * |
| 国家药典委员会: "《中华人民共和国药典2010 年版(二部)》", 1 October 2010, 中国医药科技出版社 * |
| 彭莺: "抗生素含量测定方法在《中国药典》2010年版中的应用比较", 《广东化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018098502A1 (en) * | 2016-11-28 | 2018-05-31 | Board Of Regents, The University Of Texas System | Systems, methods and devices for width-based analysis of peak traces |
| US10634653B2 (en) | 2016-11-28 | 2020-04-28 | Dionex Corporation | Systems, methods and devices for width-based analysis of peak traces |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110470748A (en) | A kind of method of the high performance liquid chromatography detection moxifloxacin hydrochloride in relation to substance | |
| CN103487518B (en) | Impurity detection method and content determination method for clindamycin hydrochloride for injection | |
| CN113588837B (en) | Detection method of moxifloxacin hydrochloride related substance | |
| CN105301159A (en) | High performance liquid chromatography analysis method of sirolimus | |
| CN103645151B (en) | A kind of method of spectinomycin content in quick mensuration spectinomycin fermented liquid or finished product | |
| CN104330495A (en) | Method for determining similarity of dissolution curves of cefdinir dry suspension | |
| CN104458984A (en) | Method for determining polymer in cephalosporins drug | |
| CN103063751A (en) | Detection method for determining polymer impurities in mezlocillin sodium | |
| CN107576735B (en) | Method for measuring high-molecular polymer in ropinan sodium | |
| Murugan et al. | A review on method development and validation by using HPLC | |
| CN105510497A (en) | Quantitative detection method of L-alanyl-L-alanine | |
| CN103604894A (en) | Method for separating and determining bortezomib chiral isomers through high-performance liquid chromatography | |
| CN103134886A (en) | Method for analytical separation sodium ibandronate and impurities thereof by utilizing high performance liquid chromatography (HPLC) | |
| CN103245741B (en) | The detection method of impurity in Meropenem | |
| CN103323541B (en) | Quality detection method for heparin sodium tube-enveloping injection | |
| CN102901783A (en) | Content determination method for pranoprofen eye drops | |
| CN102928525A (en) | Method for detecting related substance II of cefpirome in cefpirome sulfate/sodium chloride injection | |
| CN104458945A (en) | Separation and measurement method of besifloxacin hydrochloride and isomer of besifloxacin hydrochloride | |
| CN103487526A (en) | Method for detecting content of optical isomers of bortezomib | |
| CN104655757B (en) | The liquid chromatography detecting method of BC-02 | |
| CN105699507A (en) | ATP content determination kit and method thereof | |
| CN107561173B (en) | Method for measuring high-molecular polymer in Ropeinan sodium powder injection | |
| CN111337607A (en) | On-line solid phase extraction liquid chromatography for detecting kanamycin content in blood | |
| Yan et al. | Measurement of tigecycline in dried blood spots by LC–MS/MS and comparison tigecycline concentrations between whole blood and plasma | |
| CN101294935A (en) | Method for measuring SIPE content in modified polyester by reversed phase high efficiency liquid chromatography |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130424 |