CN103060554A - Vertical cell immobilized reactor for producing ferric irons by biological oxidation - Google Patents

Vertical cell immobilized reactor for producing ferric irons by biological oxidation Download PDF

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CN103060554A
CN103060554A CN2011103203606A CN201110320360A CN103060554A CN 103060554 A CN103060554 A CN 103060554A CN 2011103203606 A CN2011103203606 A CN 2011103203606A CN 201110320360 A CN201110320360 A CN 201110320360A CN 103060554 A CN103060554 A CN 103060554A
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reactor
reactor shell
oxidation
vertical cell
back taper
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CN103060554B (en
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李媛媛
张广积
曹俊雅
杨超
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Institute of Process Engineering of CAS
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Abstract

The invention discloses a vertical cell immobilized reactor for producing ferric irons by biological oxidation. The immobilized reactor is mainly composed of a round top cover (1), an upper flange (2), a reactor cylinder (3), a gas distributor (4), a lower flange (5), an inverted cone pedestal (6), a feeding inlet (7), a waste liquid-discharging port (8), an air vent (9), a sampling outlet (10), a discharging port (11) and an air hole board (12). The immobilized reactor is a cell immobilized reactor for producing the ferric irons which employs moderate thermophilic Siberian influenza bacteria as immobilized bacteria and polyurethane foam as a vector. The immobilized reactor solves the problem of low reaction rate and toxicity by toxic ions in a solution during a process of oxidizing Fe2+ into Fe3+ by bacterial oxidation in a biology process during a preoxidation treatment process of minerals during a gold production process, and can increase the oxidation rate of Fe2+ by the bacteria. The vertical cell immobilized reactor has the advantages of few bacterial contaminations, uniform and sufficient air supply, capacity for continuous operation, high oxidation rate, etc.

Description

A kind ofly produce ferric vertical cell fixation reactor for bio-oxidation
Technical field
The present invention relates to that the bacterial oxidation ferrous iron generates ferric production unit in the gold production process Minerals pre-oxidation treatment technique.
Background technology
Bioleaching is one of pretreatment technology of refractory mineral, along with rich ore constantly reduces, difficult, low-grade gold will become the main raw material that China's gold is produced, also namely become the main object of PREPROCESSING OF A REFRACTORY GOLD, low grade ore is mainly sulphide ores, and the involved microorganism of leaching process is diversified.Because the temperature of reaction of normal temperature bacterium is up to 40～45 ℃, make sulphide ores reach necessary oxidation ratio and generally need 4～5 days, and the extreme themophile major part that is grown in more than 60 ℃ lacks cell walls, be difficult to tolerate the stronger shearing force that high pulp density causes, optimum growth temp 44～55 ℃ moderate thermophile bacteria then than having higher pulp density tolerance, utmost point advantage in sulphide ores Bioleaching industrial application.The exploitation of process engineering and optimization require bacterial leaching mechanism is understood in depth.Bacterial leaching mechanism divides direct effect and indirect action, and direct effect thinks that the leaching of ore is adsorbed in mineral surface by bacterial cell, and the enzymatic by emiocytosis advances the ore dissolving; And indirect action refers to the chemical dissolution effect that occurs under ferric sulphate that ore produces and the effect of sulfuric acid become the committed step of soaking the ore deposit efficiency so produce a large amount of ferric irons in microbial metabolism.
Reaction expression is as follows:
Figure BDA0000100452600000011
Figure BDA0000100452600000012
MeS+2Fe 3+→Me 2++2Fe 2++S 0
This reactor is for indirect Leaching mechanism and two-step approach bioleaching process and design, the bioleaching process that is about to mineral is divided into the ferrous chemical process for ferric bioprocesses and ferric iron participation leaching of ores of bacterial oxidation, control respectively the suitable condition of reaction, reduce shearing action and the toxic action of mineral pair bacterium, can significantly improve leaching of ores speed.
The structure of high efficiency reactor is the key that improves biological metallurgy production efficiency, and steel basin bio-reactor and airlift bioreactor are used widely industrial.Steel basin bio-reactor gas-liquid-solid degree of mixing is higher, but ventilation that can not be at a high speed, Quick mechanical stirs the shearing force that produces has very large infringement to bacterium.Airlift bioreactor have efficient gas phase dispersion, rate of heat transfer high, mix rapid, simply constructed advantage, but gas-liquid-solid all is in flow state in the reactor, bacterium can not play consistently effect, simultaneously, when gas speed increases, stir the shearing force that causes because mixing, also can be to the bacterium injury.So the exploitation ventilation efficiency is high, bacterium can stable growth, and the reactor and the technique that reduce shearing force are present study hotspots.And the reduction of the very big degree of two-step approach bioleaching process the direct shear effect of mineral pair bacterium.In addition, in order further to reduce shearing action to the infringement of bacterium, increase the concentration of bacterium, immobilized cell technique is widely applied.Therefore, the reactor that exploitation is fit to becomes the most important thing, the vertical immobilization reactor that is used for the ferric iron oxidation is to be the reactor of the bioprocesses design of two-step approach specially, it is take polyurethane foam cheap and easy to get, as to have macroporous structure as fixation support, and take the moderate thermophile bacteria as immobilized bacterium, not only ventilation is evenly sufficient, and because immobilization role, reduced the injury of shearing force and poisonous ion pair bacterium, shorten reaction time, and the ferrous speed of bacterial oxidation is greatly improved.
Summary of the invention
The purpose of this invention is to provide a kind of vertical cell fixation reactor, slow with oxidation of divalent speed in the solution Bioleaching, the problems such as extraction time length.
Purpose of the present invention realizes by following technical proposal:
A kind of vertical immobilization reactor, by circular top cover 1, upper flange 2, reactor shell 3, gas distributor 4, lower flange 5, back taper base 6 forms, it is characterized in that described reactor shell 3 is the vertical type cylinder cylindrical shell, be provided with circular discharge port 11 above in the outer wall of reactor shell 3, reactor shell 3 belows are provided with circular thief hole 10, connect flange 2 in reactor shell 3 upper chain, upper flange 2 links with circular top cover 1, at circular top cover 1 spiracular plate 12 that distributing, what interlink with reactor shell 3 is gas distributor 4, be evenly distributed with pore on the gas distributor 4, be lower flange 5 with what connect under the gas distributor, lower flange 5 is linked with back taper base 6, is provided with ventage 9 in back taper base 6 positive bottoms, simultaneously have two holes in back taper base 6 both sides, opening for feed 7 and removal waste fluid mouth 8.
Be evenly distributed with spiracular plate 12 on the described circular top cover 1.
The upper end is provided with discharge port 11 in the described reactor shell 3, and the lower end is provided with thief hole 10.Described reactor shell 3 lower ends are connected with gas distributor 4, gas distributor 4 and lower flange 5 links.Lower flange 5 is linked with back taper base 6
Described back taper base 6 lower ends have ventilating pit 9, and both sides have opening for feed 7 and removal waste fluid mouth 8.
Described vertical immobilization reactor, used bacterial classification are the thermophilic siberian of moderate influenza bacterium.
Described vertical immobilization reactor, used fixation support are polyurethane foam, are difficult for stopping up thief hole and pore.
The present invention has following outstanding feature:
1 the present invention is with discharge port 11, and thief hole 10 and removal waste fluid mouth separately reduce living contaminants.
2 structures of the present invention have circular top cover 1, can reduce koniology, the entering of impurity.
3 the present invention have gas distributor, have avoided the part bacterium to lose activity because of lack of air, help the growth of bacterium.
4 the present invention are suitable, and density is little take polyurethane foam as fixation support, easily suspends, and is difficult for stopping up thief hole and pore, is difficult for flowing to the chemical reactor from discharge port with reaction solution.
5 the present invention are take the thermophilic siberian of moderate influenza bacterium as bacterial classification.
Description of drawings:
Fig. 1,2 is structural representation of the present invention.
Wherein, 1 is that circular top cover, 2 is that upper flange, 3 is that reactor shell, 4 is that gas distributor, 5 is that lower flange, 6 is that back taper base, 7 is that opening for feed, 8 is that removal waste fluid mouth, 9 is that ventage, 10 is that thief hole, 11 is that discharge port, 12 is spiracular plate.
Embodiment:
A kind of vertical immobilization reactor, formed by circular top cover 1, upper flange 2, reactor shell 3, gas distributor 4, lower flange 5, back taper base 6, it is characterized in that described reactor shell 3 is the vertical type cylinder cylindrical shell, be provided with circular discharge port 11 above in the outer wall of reactor shell 3, reactor shell 3 belows are provided with circular thief hole 10; Connect flange 2 in reactor shell 3 upper chain, upper flange 2 links with circular top cover 1, is uniform-distribution with pore in circular top cover 1; What interlink with reactor shell 3 is gas distributor 4, is uniform-distribution with pore on the gas distributor 4, is lower flange 5 with what connect under the gas distributor, and in order to guarantee resistance to air loss, lower flange 5 is linked with back taper base 6; Be provided with ventage 9 in back taper base 6 positive bottoms, have two holes in back taper base 6 both sides, opening for feed 7 and removal waste fluid mouth 8 simultaneously.Pass into the reaction solution reactor shell 3 of flowing through from opening for feed 7, with the immobilization bacterium reaction in the reactor shell 3, reacted liquid is got through discharge port 11.This structure can reduce shearing force, solves biooxidation rate low, and the difficult problem of pollution microbes in the experimentation is so that Bioleaching is able to serialization.
Circular top cover 1 is fixing with upper flange 2, and upper flange 2 links together with reactor shell 3
Discharge port 11 above the reactor shell 3 and thief hole 10 punch after reactor shell 3 is made and connect.
Gas distributor 4 is vertical connecting together with reactor shell 3, and gas distributor 4 links with lower flange 5, and lower flange 5 is connected together with back taper base 6.
Back taper base 6 is made first, and punching on it connects again, and connects opening for feed 7 again, ventage 9 and removal waste fluid mouth 8.
Working process of the present invention is as follows:
First in reactor shell 3, install a certain amount of carrier, prepare and contain a certain proportion of bacterium and contain 10g/L Fe 2+Substratum and pass in the back taper base 6 by opening for feed 7 with given pace, stop logical liquid after inoculation liquid rises to certain altitude in reactor shell 3 by the time.Open air compressor pump and pass into air, and regulate a certain amount of air flow quantity, regulate temperature indicator to design temperature (30 ℃～100 ℃).Reaction solution is at the pH of cylindrical shell internal reaction certain hour by thief hole 10 detection reaction liquid, and Eh is after Eh rises to 500～600mV, renew bright substratum, so loop to hang and touch experiment, until bacterium is stable in the carrier growth, in the biofilm cyclical operation, after do not add bacterium in several times the substratum.Carry out subsequently operate continuously, the substratum that pump will not contain bacterium continuously is input to the reactor shell 3 from opening for feed 7, discharge port 11 is with identical speed discharge opeing simultaneously, pH every the set time at thief hole 10 sampling and measuring solution, Eh and sampling, until Eh is stable, statistic data is calculated ferrous average rate of oxidation.
Vertical immobilization reactor is by the heating zone, and electromagnetic relay and temperature controller are controlled temperature of reaction.
Reaction solution of the present invention is for being added with Fe 2+The leathen substratum.
Embodiment one:
Loadings is 1/2 the thermophilic siberian of immobilization moderate stream bacillus S.s continuous oxidation Fe 2+Rate determination:
Polyurethane foam is cut into a certain size cubes, is loaded in the reactor shell 3 loadings 1/2.Configuration contains 10g/LFe 2+, the 0.2g/L yeast soaked the leathen substratum of powder, 10%S.s bacterium liquid, with 1: 1 H 2SO 4Regulating pH is 1.5, with pump the substratum that modulates is added in the reactor shell 3 by opening for feed 7, open air compressor pump, regulate the air robot under meter to suitable flow, between 30～100 ℃, set temperature, the operation reactor carries out bacterium immobilization experiment, when bacterium when load is stablized on carrier, carrying out operate continuously. pump is input to substratum the reactor shell 3 from opening for feed 7 continuously, simultaneously discharge port 11 is with identical speed discharge opeing, the pH every set time of 1～8h at thief hole 10 sampling and measuring solution, Eh and sampling, until Eh is stable, statistic data is calculated ferrous oxidation speed.Measuring ferrous average rate of oxidation is 1.37g/ (L.h).
Embodiment two:
Loadings is 3/4 the thermophilic siberian of immobilization moderate stream bacillus S.s continuous oxidation Fe 2+Rate determination:
Polyurethane foam is cut into a certain size cubes, is loaded in the reactor shell 3 loadings 3/4.Configuration contains 10g/LFe 2+, the 0.2g/L yeast soaked the leathen substratum of powder, 10%S.s bacterium liquid, with 1: 1 H 2SO 4Regulating pH is 1.5, with pump the substratum that modulates is added in the reactor shell 3 by opening for feed 7, open air compressor pump, regulate the air robot under meter to suitable flow, between 30～100 ℃, set temperature, the operation reactor carries out bacterium immobilization experiment, when bacterium when load is stablized on carrier, carries out operate continuously.Pump is input to substratum the reactor shell 3 from opening for feed 7 continuously, simultaneously discharge port 11 is with identical speed discharge opeing, the pH every set time of 1～8h at thief hole 10 sampling and measuring solution, Eh and sampling, until Eh is stable, statistic data is calculated ferrous oxidation speed.Measuring ferrous average rate of oxidation is 2.05g/ (L.h).

Claims (3)

1. one kind is used for bio-oxidation and produces ferric vertical cell fixation reactor, by circular top cover (1), upper flange (2), reactor shell (3), gas distributor (4), lower flange (5), back taper base (6), opening for feed (7), removal waste fluid mouth (8), ventage (9), thief hole (10), discharge port (11), pore (12) and carrier storage (13) form, it is characterized in that described reactor shell (3) is the vertical type cylinder cylindrical shell, the top is provided with circular discharge port (11) in the outer wall of reactor shell (3), and reactor shell (3) below is provided with thief hole (10); Connect flange (2) in reactor shell (3) upper chain, upper flange (2) links with circular top cover (1), is evenly distributed with pore (13) in circular top cover (1); What interlink with reactor shell (3) is gas distributor (4), is lower flange (5) with what connect under the gas distributor, and lower flange (5) is linked with back taper base (6); Be provided with ventage (9) in the positive bottom of back taper base (6), have two holes in back taper base (6) both sides, opening for feed (7) and removal waste fluid mouth (8) simultaneously.
2. vertical cell fixation reactor according to claim 1 is characterized in that used bacterial classification is the thermophilic siberian of moderate influenza bacterium.
3. vertical cell fixation reactor according to claim 1 is characterized in that used fixation support is polyurethane foam, is difficult for stopping up thief hole and pore.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016146030A1 (en) * 2015-03-13 2016-09-22 基因港(香港)生物科技有限公司 Immobile reaction device and method for reacting using immobile technique

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5211848A (en) * 1990-05-07 1993-05-18 Board Of Supervisors Of Louisiana State University Agricultural And Mechanical College Process for the microbial detoxification of toxic streams
CN201056584Y (en) * 2007-07-17 2008-05-07 中国铝业股份有限公司 Microbial leaching device
CN101921913A (en) * 2010-08-31 2010-12-22 北京矿冶研究总院 Biological pre-oxidation reactor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5211848A (en) * 1990-05-07 1993-05-18 Board Of Supervisors Of Louisiana State University Agricultural And Mechanical College Process for the microbial detoxification of toxic streams
CN201056584Y (en) * 2007-07-17 2008-05-07 中国铝业股份有限公司 Microbial leaching device
CN101921913A (en) * 2010-08-31 2010-12-22 北京矿冶研究总院 Biological pre-oxidation reactor

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016146030A1 (en) * 2015-03-13 2016-09-22 基因港(香港)生物科技有限公司 Immobile reaction device and method for reacting using immobile technique
CN106032520A (en) * 2015-03-13 2016-10-19 基因港(香港)生物科技有限公司 Immobilization reaction device and reaction method using immobilization technology
US10822581B2 (en) 2015-03-13 2020-11-03 Geneharbor (Hong Kong) Biotechnologies Limited Immobilized reaction device and a method for carrying out a reaction by utilizing the immobilization technology

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