CN103058977B - Novel method for extracting sciadopitysin from taxus mairei leaves - Google Patents
Novel method for extracting sciadopitysin from taxus mairei leaves Download PDFInfo
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- CN103058977B CN103058977B CN201210588547.9A CN201210588547A CN103058977B CN 103058977 B CN103058977 B CN 103058977B CN 201210588547 A CN201210588547 A CN 201210588547A CN 103058977 B CN103058977 B CN 103058977B
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- sciadopitysin
- extraction
- elutriant
- leaves
- white crystalline
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Medicines Containing Plant Substances (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention discloses a novel method for extracting sciadopitysin from taxus mairei leaves. The method comprises the following steps: extracting with a supercritical extraction kettle; absorbing with methanol; carrying out chromatography with AB-8 resin and 100 meshes of polyamide in sequence; collecting eluant for 9-10 min; and concentrating and drying to obtain sciadopitysin white crystalline compounds. The method is simple, and is easy to implement; and the purity of the sciadopitysin in the extracted white crystalline compounds achieves 99.36%.
Description
Technical field
The present invention relates to the extractive technique of flavones, be specifically related to a kind of method extracting Sciadopitysin from leaves of Taxus mairei.
Background technology
Containing efficacy components such as taxol, Japanese yew flavones and yew amyloses in Taxus leaf, Japanese yew flavones contains common parent nucleus C
6-C
3-C
6structure, have regulate cardiovascular system effect, anti-inflammatory and anti-immunization, anti-microbial effect, to the effect of peptide ulceration and antitumor action etc.Grant number is that the Chinese invention patent of ZL201010604150.5 discloses a kind of method extracting Sciadopitysin from southerm yew blade, extracting method comprises that blade is dried, pulverized and sieved, ultrasonic extraction, after extraction, extracting solution first adds chloroform, AB-8 macroporous resin chromatography, chloroform, methyl alcohol and ethyl acetate mixed solution wash-out, recycle silicon plastic column chromatography after wash-out is concentrated, with by chloroform, methyl alcohol and ethyl acetate mixed solution wash-out, isolate the elutriant of 27 ~ 29min, concentration drying obtains the Sciadopitysin that purity reaches 98.76%.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method extracting Sciadopitysin from leaves of Taxus mairei, and the method is easier, extracts the Sciadopitysin monomer purity obtained higher.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of method extracting Sciadopitysin from leaves of Taxus mairei, and its step is as follows:
1, fresh southerm yew blade is dried 24h at 50 DEG C of temperature, pulverize, crossing No. 8 sieves is powder, loads in supercritical extraction reactor;
2, in supercritical extraction reactor, be 30 DEG C in extraction temperature, CO
2hydrodynamicpressure is 25MPa, under entrainment agent ethanol flow velocity 0.25ml/min, and extraction 1.5h, extraction is extracted thing;
3, in extract, add methanol absorption is absorption liquid, the addition of described methyl alcohol is add methyl alcohol 1ml in the extract of above-mentioned every 5g powder, absorption liquid AB-8 resin chromatography, then be the ethanol elution of 80% by mass percentage concentration, obtain the first elutriant, first elutriant is concentrating under reduced pressure 60min under 500Pa, obtain the first concentrated solution, first concentrated solution, 100 order polyamide chromatography, be the ethanol elution of 40% by mass percentage concentration again, collect the elutriant of 9 ~ 10min, concentrate drying, obtain white crystalline compound, this white crystalline compound is Sciadopitysin.
Compared with prior art, the invention has the advantages that a kind of method extracting Sciadopitysin from leaves of Taxus mairei, extracted by supercritical extraction reactor, methanol absorption, after AB-8 resin chromatography, use 100 order polyamide chromatography again, collect the elutriant of 9 ~ 10min, concentrate drying obtains Sciadopitysin white crystalline compound, this simple and convenient extraction, easy to implement, in the white crystalline compound of extraction, the purity of Sciadopitysin reaches 99.36%.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment
From leaves of Taxus mairei, extract a method for Sciadopitysin, first fresh southerm yew blade is dried 24h under 50 DEG C of conditions, pulverize, crossing No. 8 sieves is powder, and weigh 50g, loads in supercritical extraction reactor; Be 30 DEG C in extraction temperature in supercritical extraction reactor, CO
2hydrodynamicpressure is 25MPa, under entrainment agent ethanol flow velocity 0.25ml/min, extraction 1.5h, adding 10ml methanol absorption in extract is absorption liquid, absorption liquid AB-8 resin (commercially available) chromatography, AB-8 resin is low-pole, surface-area 480 ~ 520 (m2/g), particle radii 13 ~ 14nm, water content 64%; Be the ethanol elution of 80% by mass percentage concentration after chromatography, obtain the first elutriant, concentrating under reduced pressure 60min under first elutriant 500Pa, obtain the first concentrated solution, first concentrated solution, 100 order polymeric amide (commercially available) chromatographies, be the ethanol elution of 40% by mass percentage concentration again, collect the elutriant of 9 ~ 10min, concentrate drying, obtain white crystalline compound, this white crystalline compound is Sciadopitysin, and adopt high effective liquid chromatography for measuring, in white crystalline compound, Sciadopitysin purity reaches 99.36%.
Claims (1)
1. from leaves of Taxus mairei, extract a method for Sciadopitysin, it is characterized in that step is as follows:
1) fresh southerm yew blade is dried 24h at 50 DEG C of temperature, pulverize, crossing No. 8 sieves is powder, loads in supercritical extraction reactor;
2), in supercritical extraction reactor, be 30 DEG C in extraction temperature, CO
2hydrodynamicpressure is 25MPa, under entrainment agent ethanol flow velocity 0.25ml/min, and extraction 1.5h, extraction is extracted thing;
3) in extract, add methanol absorption is absorption liquid, the addition of described methyl alcohol is add methyl alcohol 1ml in above-mentioned every 5g powder gained extract, absorption liquid AB-8 resin chromatography, then be the ethanol elution of 80% by mass percentage concentration, obtain the first elutriant, first elutriant is concentrating under reduced pressure 60min under 500Pa, obtain the first concentrated solution, first concentrated solution, 100 order polyamide chromatography, be the ethanol elution of 40% by mass percentage concentration again, collect the elutriant of 9 ~ 10min, concentrate drying, obtain white crystalline compound, this white crystalline compound is Sciadopitysin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210588547.9A CN103058977B (en) | 2012-12-31 | 2012-12-31 | Novel method for extracting sciadopitysin from taxus mairei leaves |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210588547.9A CN103058977B (en) | 2012-12-31 | 2012-12-31 | Novel method for extracting sciadopitysin from taxus mairei leaves |
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| CN103058977A CN103058977A (en) | 2013-04-24 |
| CN103058977B true CN103058977B (en) | 2015-06-03 |
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| CN201210588547.9A Expired - Fee Related CN103058977B (en) | 2012-12-31 | 2012-12-31 | Novel method for extracting sciadopitysin from taxus mairei leaves |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818257A (en) * | 2016-12-12 | 2017-06-13 | 浙江大学宁波理工学院 | Improve the regulation and control method of yew Flavonoid substances in leaves of Taxus mairei |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070004649A1 (en) * | 2003-02-25 | 2007-01-04 | The Kitasato Institute | Anti-influenza virus compound comprising biflavonoid-sialic acid glycoside |
| CN101982171A (en) * | 2010-11-06 | 2011-03-02 | 新疆维吾尔自治区药物研究所 | Application of fenugreek biflavone glycosides for preparing anti-virus or/and anti-tumor drugs |
| CN102070589A (en) * | 2010-12-24 | 2011-05-25 | 宁波泰康红豆杉生物工程有限公司 | Method for extracting sciadopitysin from laxus malrel leaves |
| CN102258514A (en) * | 2010-05-25 | 2011-11-30 | 中国人民解放军第二军医大学 | Application of sciadopitysin in preparing medicines and food for preventing and treating osteoporosis |
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2012
- 2012-12-31 CN CN201210588547.9A patent/CN103058977B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070004649A1 (en) * | 2003-02-25 | 2007-01-04 | The Kitasato Institute | Anti-influenza virus compound comprising biflavonoid-sialic acid glycoside |
| CN102258514A (en) * | 2010-05-25 | 2011-11-30 | 中国人民解放军第二军医大学 | Application of sciadopitysin in preparing medicines and food for preventing and treating osteoporosis |
| CN101982171A (en) * | 2010-11-06 | 2011-03-02 | 新疆维吾尔自治区药物研究所 | Application of fenugreek biflavone glycosides for preparing anti-virus or/and anti-tumor drugs |
| CN102070589A (en) * | 2010-12-24 | 2011-05-25 | 宁波泰康红豆杉生物工程有限公司 | Method for extracting sciadopitysin from laxus malrel leaves |
Non-Patent Citations (2)
| Title |
|---|
| 超临界流体萃取红豆杉中有效成分的工艺优化;唐倩等;《重庆医学》;20101231;第39卷(第23期);第3173页摘要，第3174页第2节"结果"部分 * |
| 高昕.东北红豆杉枝叶中黄酮类成分富集工艺研究.《黑龙江中医药大学硕士学位论文》.2005,第14页第1.5节"超临界流体提取法"部分,第15页第2.3节"聚酰胺吸附法"部分,第36页. * |
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