CN103048426A - Method for analysis and determination by measuring content of amino acids and proteins in food and feed - Google Patents
Method for analysis and determination by measuring content of amino acids and proteins in food and feed Download PDFInfo
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- CN103048426A CN103048426A CN2012105683369A CN201210568336A CN103048426A CN 103048426 A CN103048426 A CN 103048426A CN 2012105683369 A CN2012105683369 A CN 2012105683369A CN 201210568336 A CN201210568336 A CN 201210568336A CN 103048426 A CN103048426 A CN 103048426A
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Abstract
The invention discloses a method for analysis and determination by measuring the content of amino acids and proteins in food and feed. The content of proteins is measured by a kjeldah method and the content of various free and hydrolytic amino acids and the total content of amino acids are measured by an amino acid analyzer; a lot of samples are analyzed fundamentally to form a database and a judgment system; and each measured result is analyzed and compared with system data to judge whether fake proteins are adulterated in the food and the feed. By the method, a precise judgment can be made that components of non-proteins or fake proteins are adulterated in the sample only if one or more of the following measured data is abnormal: content of non-proteins, content of free amino acids, content of characteristic amino acids, percentage between the total content of amino acids and the proteins, the ratio of each component of the amino acids to the total content of the amino acids and the like, so that the quality safety of the food and the feed are ensured.
Description
Technical field
The present invention relates to food and feed industrial detection field, specifically be a kind of method of analyzing judgement by the content of measuring amino acid and protein in the food and feed.
Background technology
At present, the national standard method of measuring protein content in the food and feed mainly is Kjeldahls method, its disadvantage is first from the subjective blind area that object is supposed in advance that exists, protein is together measured and be scaled to the nitrogenous thing of non-albumen that sample itself is contained or add and the nitrogen in the protein, and the result makes the sample determination value that contains nonprotein nitrogen obviously more higher than protein actual content; The method more can not be told the false albumen of adulterating, mixing the spurious with the genuine.Although utilize amino-acid analyzer to measure the impact that method of protein can be got rid of non-protein substance, still can't identify fully for the interference of false albumen.Just because of each method all can have its shortcomings and limitations, can not measure the real content of protein in the product fully, some lawless persons utilize this point just, in food or feed, mix common method and be difficult for detected false albumen or non-protein substance, and also increasingly complex of the material that mixes and means, thereby wish by a kind of method just can get rid of the interference of false albumen or non-protein substance, the idea that also accurately detects protein content is difficult to realize.
Summary of the invention
The object of the present invention is to provide a kind of method of analyzing judgement by the content of measuring amino acid and protein in the food and feed, can analyze rapidly and accurately by the method whether the situation of mixing non-albumen or false protein substance is arranged in the judgement sample.
A kind of method of analyzing judgement by the content of measuring amino acid and protein in the food and feed provided by the invention, it is characterized in that utilizing Kjeldahl nitrogen determination protein content and amino-acid analyzer to measure various amino acid contents and total amino acid content free and hydrolysis, by to the fundamental analysis of a large amount of samples and form database and the judgement system, result and the system data measured are analysed and compared at every turn, thereby judge whether have protein to mingle in the food and feed.
Under normal circumstances, the non-protein nitrogen content of one matter, free aminoacid content, feature amino acid content, each proportionate relationship that forms of amino acid etc. are relatively-stationary, grasped these data and with its analysis, conclusion, statistics and formation system, just can analyze whether the situation of mixing non-albumen or false protein substance is arranged in the judgement sample.The method that content by amino acid and protein in the mensuration food and feed provided by the invention is analyzed judgement comprises following concrete steps:
(1) using the Kjeldahl nitrogen determination protein content is crude protein content;
(2) use amino-acid analyzer to measure various amino acid contents and total amino acid content free and hydrolysis;
(3) by the measurement result of the above-mentioned project of a large amount of samples is carried out analytic statistics, and take as the data basis, make up protein test macro database;
(4) be hydrolyzed the number percent that rear total amino acid content accounts for crude protein content by calculating, compare with system data again, judge whether to be added with non-albumen nitrogen-containing compound;
(5) free aminoacid content by measuring is analyzed comparison with the free aminoacid content of product in the system own, judges whether to be added with exogenous amino acid;
(6) by measuring feature amino acid, compare with system, judge whether to be added with other protein ingredient;
(7) by calculating the ratio of each amino acid whose content and total amino acid content after the hydrolysis, each amino acid content accounts for the ratio range of total amino acid content in the comparison system again, judges whether to be added with other protein substance.
Reality of the present invention is only used Kjeldahls method and two kinds of common methods of amino-acid analyzer method, by to the fundamental analysis of a large amount of samples and form database and the judgement system, result and the system data measured are analysed and compared at every turn, just can judge whether that protein mingles, thereby guarantee food and quality of the fodder safety.
Embodiment
The method that the content that passes through amino acid and protein in the mensuration food and feed that the specific embodiment of the invention provides is analyzed judgement comprises following concrete steps: (1) uses the Kjeldahl nitrogen determination protein content is crude protein content; (2) use amino-acid analyzer to measure various amino acid contents and total amino acid content free and hydrolysis; (3) by the measurement result of the above-mentioned project of a large amount of samples is carried out analytic statistics, and take as the data basis, make up protein test macro database; (4) be hydrolyzed the number percent that rear total amino acid content accounts for crude protein content by calculating, compare with system data again, judge whether to be added with non-albumen nitrogen-containing compound; (5) free aminoacid content by measuring is analyzed comparison with the free aminoacid content of product in the system own, judges whether to be added with exogenous amino acid; (6) by measuring feature amino acid, compare with system, judge whether to be added with other protein ingredient; (7) by calculating the ratio of each amino acid whose content and total amino acid content after the hydrolysis, each amino acid content accounts for the ratio range of total amino acid content in the comparison system again, judges whether to be added with other protein substance.
Embodiment 1
Certain feed is 47.6% with Soybean Meal by the thick protein that Kjeldahls method records, and meets the requirement of " thick protein 〉=44.0% " of the middle regulation of one-level belt leather Soybean Meal in GB/T19541-2004 " feed Soybean Meal " standard; And the method that provides by the specific embodiment of the invention is 34.0% with the 16 seed amino acid total amounts that amino-acid analyzer records after the hydrolysis, the total amino acid content that will record with amino-acid analyzer is divided by the gross protein value that records with Kjeldahls method, drawing the number percent that total amino acid content accounts for gross protein value is 71.4%, the total amino acid content that draws by the fundamental analysis of a large amount of dregs of beans sample accounts for the normal number percent of thick protein all greater than 85%, and the sample number percent of this time measuring is obviously on the low side, interpret sample can not generate amino acid whose nitrogen substance existence after being mixed with hydrolysis, this part nitrogen substance can be calculated to be in the protein content mistakenly with Kjeldahls method, therefore can judge by the native system method and to have added non-albumen nitrogen-containing compound in the sample.
Embodiment 2
It is 1.13g/100mL that certain milk-contained drink records protein content by the Kjeldahls method of national standard, meets the requirement of " protein 〉=1.0g/100mL " of regulation among the GB11673-2003 " milk-contained drink hygienic standard "; Reach 0.33g/100mL and detect its free glycine content of discovery by the method that the specific embodiment of the invention provides, deduct free glycine content with total protein content, the protein content of milk-contained drink reality is no more than 0.8g/100mL as can be known, obviously do not meet the national standard requirement, and the protein in the milk-contained drink all comes from lactoprotein, contain hardly free glycine, can be with this free glycine as protein and cause erroneous judgement with Kjeldahls method, so can judge by the native system method and to have added exogenous amino acid (glycocoll) in this sample.
Embodiment 3
It is 30.8g/100g that certain milk powder records protein content by Kjeldahls method, meets the relevant criterion requirement; And adopt amino-acid analyzer to record the hydroxyl dried meat of 0.82g/100g by the method that the specific embodiment of the invention provides
Propylhomoserin, hydroxyproline is the feature amino acid of animal collagen, contains the hydroxyproline of more amount in the collagen, and normal milk powder does not then contain hydroxyproline, this detects the hydroxyproline of 0.82g/100g this milk powder, can illustrate to be added with the collagen composition in this milk powder.
Embodiment 4
Certain animal fodder fish meal is 68.0% by the crude protein that Kjeldahls method records, and meets the requirement of " crude protein 〉=65% " of select quality regulation in GB/T19164-2003 " fish meal " standard; The 16 seed amino acid total amounts that record with amino-acid analyzer are 60.6%, total amino acid content accounts for 89.1% of crude protein content, also zone of reasonableness (zone of reasonableness that draws through a large amount of fish meal sample fundamental analysiss is that total amino acid content accounts for the ratio of crude protein content greater than 85%); But measure aspartic acid by the methods analyst that the specific embodiment of the invention provides, serine, glutamic acid, glycocoll, methionine, lysine, histidine, the value of proline, again it is drawn its shared ratio divided by total amino acid content respectively, calculate to get aspartic acid, glutamic acid, methionine, lysine, the ratio that the amino acid contents such as histidine account for total amino acid content is starkly lower than normal level, serine, glycocoll, proline etc. account for the ratio of total amino acid content apparently higher than normal level (normal level is by forming by the drawn result's statistics of a large amount of fundamental analysiss), can judge other protein substance that has added non-fish meal in this sample by the native system method.
Claims (2)
1. method of analyzing judgement by the content of measuring amino acid and protein in the food and feed, it is characterized in that utilizing Kjeldahl nitrogen determination protein content and amino-acid analyzer to measure various amino acid contents and total amino acid content free and hydrolysis, by to the fundamental analysis of a large amount of samples and form database and the judgement system, result and the system data measured are analysed and compared at every turn, thereby judge whether have protein to mingle in the food and feed.
2. the content by amino acid and protein in the mensuration food and feed according to claim 1 is analyzed the method for judgement, it is characterized in that comprising following concrete steps:
(1) using the Kjeldahl nitrogen determination protein content is crude protein content;
(2) use amino-acid analyzer to measure various amino acid contents and total amino acid content free and hydrolysis;
(3) by the measurement result of the above-mentioned project of a large amount of samples is carried out analytic statistics, and take as the data basis, make up protein test macro database;
(4) be hydrolyzed the number percent that rear total amino acid content accounts for crude protein content by calculating, compare with system data again, judge whether to be added with non-albumen nitrogen-containing compound;
(5) free aminoacid content by measuring is analyzed comparison with the free aminoacid content of product in the system own, judges whether to be added with exogenous amino acid;
(6) by measuring feature amino acid, compare with system, judge whether to be added with other protein ingredient;
(7) by calculating the ratio of each amino acid whose content and total amino acid content after the hydrolysis, each amino acid content accounts for the ratio range of total amino acid content in the comparison system again, judges whether to be added with other protein substance.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107515241A (en) * | 2017-07-26 | 2017-12-26 | 广东省食品工业研究所有限公司 | A kind of method quickly detected simultaneously to various heavy in feed |
| CN108088968A (en) * | 2017-11-23 | 2018-05-29 | 广东省食品工业研究所有限公司 | A kind of discrimination method of schefflera honey adulteration |
| CN109001470A (en) * | 2018-09-30 | 2018-12-14 | 南京财经大学 | A kind of precise determination method of protein content in edible mushroom |
| CN110546499A (en) * | 2017-02-13 | 2019-12-06 | 赢创德固赛有限公司 | method for determining the processing impact on the nutritional value of feed stocks |
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- 2012-12-24 CN CN2012105683369A patent/CN103048426A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110546499A (en) * | 2017-02-13 | 2019-12-06 | 赢创德固赛有限公司 | method for determining the processing impact on the nutritional value of feed stocks |
| CN107515241A (en) * | 2017-07-26 | 2017-12-26 | 广东省食品工业研究所有限公司 | A kind of method quickly detected simultaneously to various heavy in feed |
| CN107515241B (en) * | 2017-07-26 | 2020-03-24 | 广东省食品工业研究所有限公司 | Method for rapidly detecting multiple heavy metals in feed simultaneously |
| CN108088968A (en) * | 2017-11-23 | 2018-05-29 | 广东省食品工业研究所有限公司 | A kind of discrimination method of schefflera honey adulteration |
| CN109001470A (en) * | 2018-09-30 | 2018-12-14 | 南京财经大学 | A kind of precise determination method of protein content in edible mushroom |
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Application publication date: 20130417 |