CN103048265A - Method for observing dolycoris baccarum - Google Patents
Method for observing dolycoris baccarum Download PDFInfo
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- CN103048265A CN103048265A CN2012104968690A CN201210496869A CN103048265A CN 103048265 A CN103048265 A CN 103048265A CN 2012104968690 A CN2012104968690 A CN 2012104968690A CN 201210496869 A CN201210496869 A CN 201210496869A CN 103048265 A CN103048265 A CN 103048265A
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Abstract
The invention discloses a method for observing a dolycoris baccarum. The method comprises the following steps of: (1) soaking a dolycoris baccarum body in alcohol; (2) taking out a dolycoris baccarum sample soaked in the alcohol, carrying out microscopic examination below an anatomical lens, and manufacturing a glass slide; and (3) observing the manufactured sample glass slide below an optical microscope. The method provided by the invention has the advantages in that the characters of the dolycoris baccarum can be rapidly observed, the age of the dolycoris baccarum is analyzed, so that a suitable method for treating the dolycoris baccarum can be found out; and the damage on crops is reduced.
Description
Technical field
The invention belongs to the entomological taxonomy field, particularly a kind of method of observing spot palpus stinkbug.
Background technology
Spot palpus stinkbug, formal name used at school Dolycoris baccarum (Linnaeus) belongs to the Semiptera Pentatomiddae, claims again sloe bug, the popular name for a kind of stinkbug.All there is distribution all parts of the country, are one of the widest kinds of Pentatomiddae distribution, the plants such as harm various vegetables, medicinal material.The host mainly contains wheat, barley, grain (millet), corn, Chinese cabbage, rape, wild cabbage, radish, pea, carrot, green onion and other crops.Adult and nymph thorn are inhaled tender leaf, tender stem and fringe section juice.After cauline leaf is killed, the tawny spot appears, leaf rolling when serious, the wilting contracting of tender stem, impact growth, the underproduction reduction of income.
Spot must become long 8～13.5 millimeters of polypide by stinkbug, and is wide approximately 6 millimeters, ellipse, and tawny or puce, body is carried on the back the white fine hair of close quilt and the little punctum of black; Beak is elongated, is close to the head outside of belly.The pronotary front is light yellow, the rear portion dark yellow.The nearly triangle of escutcheon, terminal blunt and smooth, yellow-white.Fore wing corium bronzing, the diaphragm tawny, transparent, surpass abdomen end.The outside of belly of chest belly is filbert, scatters fragmentary pore, sufficient tawny, leg section and the tibia black punctum that gathers.Ovum grain cylindrical shape, primiparity is light yellow, presents lark when closely hatching, and chorion has reticulate pattern, gives birth to white down.The ovum marshalling becomes piece.
Spot must stinkbug be met low temperature and is often stopped action, and hides in the plant bottom, and in phyllopodium or the ground soil seam, weather is fine when warm, the plant top that then crawls toward, and frequently circle in the air, mating and laying eggs.When noon in summer was hot, adult also often hid in plant bottom, blade back, ground soil seam to hide high temperature, and took food when sooner or later nice and cool, mating, lays eggs.Rainfall and high temperature are unfavorable for that spot must stinkbug existence.The farmland that growing way is good, spot must be moved into more early by stinkbug, and generating capacity is also larger.
Must the stinkbug annual generation of spot is different because of the area, 1～2 generation of the Yellow River northern area, 3 ~ 4 generations of areas to the south, the Changjiang river.Survive the winter in weeds in field, dry branches and fallen leaves, plant rhizosphere, bark crack and the inferior shelter of eaves with adult.Come into play at the beginning of 4 months, mid-April, mating was laid eggs, by the end of April primary larva hatching in May, and first generation adult sprouted wings at the beginning of 6 months, and be spawning period mid-June; The second generation begins to turn into adult mid-August in the larva hatching of early July mid or late June, and October, early and middle ten days was survived the winter successively.Betide mid-April to mid-July in the Huanghuai Area first generation, the second generation betides late June to mid-September, and the third generation betides mid-July until early June in next year.Later stage generation overlap phenomenon is obvious.
1 day namely begins to take food behind the spot palpus stinkbug adult eclosion, carries out mate and oviposit in 3 ~ 4 days, and this phase causes harm the heaviest.The faint photoaxis of adult tool was dwelt in blade back under strong sunshine more, and weak sunshine or cloudy day, then many at tender leaf or bud, thorn is inhaled juice on the young fruit, takes food and causes harm.
Spot must stinkbug be the polyphagy insect, and adult must suck the floral organ nutriment of host plant, could breed by normal spawning, the ovum consor becomes piece, every 12 ~ 24, how ovum is produced on the young tender positions such as bag sheet of crop upper blade front or bud or fruit, be the multirow proper alignment.Just incubate the nymph harm of trooping, diffusion harm after 2 ages.Adult and nymph foul smelling, all happiness is clustered in the tender part of crop children and juice is sucked by fringe section, continues harm from spring to the autumn.
The spot scope that must stinkbug causes harm is wide, and the floristics of causing harm is many, causes crop failure, and economic benefit is impaired.Spot must stinkbug be the de novo a kind of insect of in recent years agricultural production of Heilongjiang Province, mainly cause harm corn, beet and potato.Only indivedual cities and counties work the mischief spot palpus stinkbug in 2000 in the Heilongjiang Province, and calendar year 2001 is developed into more than 20 cities and counties such as Suihua, Harbin, and approximately 41.3 ten thousand hectares of areas occur, and this worm still works the mischief in some areas after 2002.
The stinkbug class pest that vega is common must occur comparatively general by stinkbug with spot.According to interrelated data, in recent years in Anhui, cigarette district, the ground harm such as Shandong, Henan are on the rise.During spot palpus stinkbug harm tobacco, one-tenth, nymph all inhale juice with sucking mouth parts at top blade or tender stem thorn, and down to being stayed yellow-white to the dusky spot by the thorn place, particularly its saliva has destruction to plant tissue.
Blackberry, blueberry is one of third generation fruit of recommending to the world of the World Food Programme (FAO) 1993.Nineteen ninety-five from U.S.'s successful introduction, by the Municipal Commission of Science and Technology, the city Bureau of Finance was classified agriculture key popularized project in 1997 by the Xuecheng District Xing Nong of Zaozhuang City, Shandong Province seeds company, was dark " pollution-free food " popular to people.In recent years, find in blackberry, blueberry production garden, Xuecheng District Nan Shi town, spot must endanger blackberry, blueberry by stinkbug to some extent.Be the harm Sheng phase 5～July.One-tenth, nymph happiness inhabit thorn suction fruit juice between fruit ear, sometimes have bull one-tenth, nymph to strive the food phenomenon on the fruit.In 4 ~ May, spot must stinkbug harm plant tender shoots, cause that spire is withered, young sprout " broken is crazy ".Blue or green tender young fruit is injured, and causes the fruit grain normal development not expand, and becomes malformed fruit.Mellow fruit is stung suction juice, and fine dry days, the fruit grain withered flatly, and the fruit grain is matt; Chance rainwater fruit grain fades rotten, becomes the albefaction fruit, and the mouthfeel variation loses commodity value.Generally cause the underproduction 5%, seriously can reach about 15%.
By inquiry, this worm has sudden, and agricultural production is consisted of larger threat, should take effectively to combine anti-measure, alleviates or stops the loss that this worm is caused agricultural production, promotes Agriculture Production.At present, to spot must stinkbug feature identification correlative study between each nymph in age less, in actual production, be difficult to find foundation.
The method of preventing and treating of spot palpus stinkbug is varied.In cultural control, do the field health well, in time remove weeds in field and dry branches and fallen leaves, adult partial wintering can be eliminated in winter ploughing and cleaning vegetable plot, reduces the insect population of surviving the winter.Spawning period examines, nip in the bud pieces of an egg and first hatching nymph, but also Artificially taking away the egg cases.When adult occurs, utilize its seemingly-dead property, the vibration plant after landing kills its rapid seizure.The chemical control aspect, emphasis spray medicine control adult and 1,2 age nymph.Control is 1～2 time when nymph or adult have just moved the farmland.The medicament that can Gong select has 80% metrifonate soluble powder or 1000 ~ 1500 times of liquid of 40% dimethoate emulsifiable concentrate, 2000 times of liquid of 48% chlopyrifos (Le Siben), 2.5% deltamethrin milk oil, or 20% fenvalerate, or 3000 ~ 4000 times of liquid of 2.5% time missible oil etc., interval 7 ~ 10 days.Spot must have many kinds of natural enemies by stinkbug, and its Chinese Ji hunts stinkbug can prey on nymph, but the egg parasitoids egg parasitoid can both play certain control action.
Therefore, we should comprehensively understand the worm age of spot palpus stinkbug, just can reach the purpose of omnibearing this insect of control.Spot palpus stinkbug is more close in the body surface architectural feature in each nymph stage, is difficult for difference, especially 1,2 length of time.
Summary of the invention
The purpose of this invention is to provide a kind of method of observing spot palpus stinkbug, can observe fast the feature of each worm section in age of spot palpus stinkbug.
Adopt for achieving the above object following technical proposals: a kind of method of observing spot palpus stinkbug comprises the steps:
(1) spot must be soaked in the stinkbug polypide alcohol;
The spot that (2) will be soaked in the alcohol must take out by the stinkbug sample, and microscopy under anatomical lens is made slide;
(3) the sample slide of making is placed the optical microphotograph Microscopic observation.
Described making slide comprises the steps:
(1) each several part of the integral body of nymph or adult being put into 10%～15% sodium hydroxide solution soaked 5～8 hours; Adult all takes off head, wing and the foot of adult first, with scissors or blade the both side edges of adult polypide is cut, pull open gently backboard and web with tweezers, internal organ and muscle are all removed, clean out, again the periproct connecting place is cut off, obtain pronotary, messcutellum, abdominal terga, the belly web of adult.
(2) use the clear water soaking and washing until clean with the intrinsic sewage exchange of mark;
(3) with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide;
(4) with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters the whole appearance while dewatering step by step;
(5) drip an amount of dimethylbenzene transparent, with thieving paper blot clean after, drip neutral resins, the cover glass mounting.
Advantage of the present invention is the feature that can observe out fast spot palpus stinkbug, analyzes the worm age of spot palpus stinkbug, in order to find suitable method to process spot palpus stinkbug, reduces the injury to crops.
Embodiment
Embodiment 1
Adult is soaked in alcohol, dissected.When dissecting adult, head, wing and foot with adult all takes off first, with scissors or blade the both side edges of adult polypide is cut, pull open gently backboard and web with tweezers, internal organ and muscle are all removed, clean out, again the periproct connecting place is cut off, obtain pronotary, messcutellum, abdominal terga and belly web.
With the spot of dismembering out must the stinkbug adult pronotary, messcutellum, abdominal terga, the belly web is put into 10%～15% sodium hydroxide solution and was soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
The body surface architectural feature of spot palpus stinkbug adult polypide: adult feeler 5 joints, black, each saves the end and base portion is faint yellow, darkly yellow alternate characteristics.There is the intensive hair together of cluster at front foot leg section middle part, and is comparatively obvious when observing.
The punctum that gathers on adult pronotary and the messcutellum, between the pronotary punctum give birth to the hair of many upright elongate, less hair is arranged on the messcutellum.The pronotary lateral margin of polypide is smooth, without thrust.
Adult abdominal terga black without hair, has many thorn-like kicks.The belly web scatters stain, and gives birth to sparse elongated pubescence, and than the pronotary capillary, the shape of hair is basic identical.Venter 3 to the 8 uromere side edge region of adult can find to have the hair of special base portion class floweriness shape, and every joint has two, and the size of base portion is far smaller than valve, and 1,2 liang of uromere side edge region only has small undercoat without this type hair.
Embodiment 2
Must place alcohol to soak by the stinkbug nymph on spot, the whole polypide of nymph is put into 10%～15% sodium hydroxide solution and soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
One age nymph body surface architectural feature
One age nymph feeler 4 joint, light brown, each internode is basically identical with joint middle part color.There is the intensive hair together of cluster at front foot leg section middle part, and can be observed quantity is 4.
The polypide integral color is more shallow, villous on the chest backboard.Pronotary lateral margin the smooth of the edge does not have projection.
Also villous on the belly back side, abdominal terga light color, each saves central authorities and there is the black patch both sides, and three black regions of 2 to 5 uromere backboard central authorities are the scent gland opening, and a small amount of hair is arranged on it.Abdominal terga both sides black patch is the connexivum zone, can see hair different in size on each connexivum, and the long hair of every joint has 2, also has some undercoats.
The belly web is light yellow, and sparse small fine hair is arranged, and is short than adult belly web hair, and venter 3 to 7 uromere side edge region have the hair of special base portion class floweriness shape, and every joint only has one, and the base portion size is similar to the valve on next door.
Embodiment 3
Must place alcohol to soak by the stinkbug nymph on spot, the whole polypide of nymph is put into 10%～15% sodium hydroxide solution and soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
Two age nymph body surface architectural feature
Two age nymph feeler 4 joint, light brown, each internode is basically identical with joint middle part color, with one age nymph identical.There is the intensive hair together of cluster at front foot leg section middle part, and the position is identical with an age, and can be observed quantity is 6, than one age nymph many.
On the polypide chest backboard sparse undercoat is arranged, the pronotary lateral margin begins to have the zigzag microspike.
Also there is sparse undercoat at the belly back side.Identical with an age is, two age the nymph belly back side respectively save central authorities and there is the black patch both sides, three black regions of 2 to 5 uromere backboard central authorities are the scent gland opening, the quantity of hair is than increasing to some extent an age.The both sides black region is connexivum, can see hair different in size on each connexivum, and the long hair of every joint has 3, than 1 of a nymph increase in age.
The belly web has sparse undercoat.Two age nymph venter 3 to 7 uromere side edge region, the hair of special base portion class floweriness shape is arranged, every joint has 2, than one age many 1 of nymph, the base portion size is similar to the valve on next door.
Embodiment 4
Must place alcohol to soak by the stinkbug nymph on spot, the whole polypide of nymph is put into 10%～15% sodium hydroxide solution and soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
Tritonymph body surface architectural feature
Tritonymph feeler 4 joints, brown, each internode is basically identical with joint middle part color.There is the intensive hair together of cluster at front foot leg section middle part, and can be observed quantity is 8, and is many than an age, two ages.
The hair of polypide chest backboard becomes intensive, and the base portion of hair darkens.The pronotary lateral margin serration of polypide is more obvious.
The quantity of tritonymph belly back side hair also than an age, two age nymph increase.Central authorities are respectively saved at the belly back side and there is the black patch both sides, and three black regions of 2 to 5 uromere backboard central authorities are the scent gland opening, the hair of giving birth to and become many, and the both sides black region is connexivum, and the hair on it is than also increasing to some extent for two ages.
Hair beginning on the belly web is elongated, and venter 3 to 7 uromere side edge region the hair of the base portion class floweriness shape of giving birth to, with an age, two age nymph shape identical, adnation position is also identical, at 3 to 7 uromeres, with two age nymph identical, 2 at every joint, the base portion size is similar to the valve on next door.Equally, around the 8th uromere, only have a valve, do not have the hair of base portion class floweriness shape.
Embodiment 5
Must place alcohol to soak by the stinkbug nymph on spot, the whole polypide of nymph is put into 10%～15% sodium hydroxide solution and soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
Four age nymph body surface architectural feature
Four age nymph feeler 4 joint, taupe, each internode is basically identical with joint middle part color.Front foot leg section middle part has the intensive hair together of cluster equally.
Polypide chest backboard begins to occur punctum, pronotary lateral margin serration than 3 age nymph the projection degree little.
Four age the nymph belly back side respectively save central authorities and both sides and also be the black patch, three black regions of 2 to 5 uromere backboard central authorities are the scent gland opening, the wool base of giving birth to and become large, on slide sample, the base portion of hair has the zone of double-ring, than one, two, tritonymph is different.Both sides, belly back side black patch is the connexivum zone, the base portion color burn of hair on it.
Venter 3 to 7 uromere side edge region the hair of the base portion class floweriness shape of giving birth to, still with two, tritonymph is identical, at 3 to 7 uromeres, 2 at every joint, the base portion size is similar to the valve on next door.Equally, around the 8th uromere, only have a valve, do not have the hair of base portion class floweriness shape.The visible a pair of wing bud in the polypide back side extends between promerous and the second uromere.
Embodiment 6
Must place alcohol to soak by the stinkbug nymph on spot, the whole polypide of nymph is put into 10%～15% sodium hydroxide solution and soaked 5～8 hours, with the clear water soaking and washing until clean with the exchange of the intrinsic sewage of mark, with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide, with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters step by step, the whole appearance while dewatering, drip subsequently an amount of dimethylbenzene transparent, after blotting only with thieving paper, drip neutral resins, the cover glass mounting.
The sample slide of making is placed the optical microphotograph Microscopic observation.
Five age nymph body surface architectural feature
Five age nymph feeler 4 joint, tawny, each internode is basically identical with joint middle part color.Front foot leg section middle part has the intensive hair together of cluster equally.
The densely covered black punctum of polypide body surface and white fine hair.Chest backboard punctum is intensive, and the hair between punctum is also a lot, than increasing for four ages.The pronotary lateral margin serration of polypide disappears, the smooth of the edge.
The quantity of belly back side hair increases severely.Five age the nymph belly back side respectively save central authorities and both sides and also be the black patch, three black regions of 2 to 5 uromere backboard central authorities are the scent gland opening, the hair of giving birth to intensive.The black patch zone internal hair quantity of connexivum increases greatly.
Five age nymph the hair of uromere connexivum outside of belly base portion class floweriness shape, compared significant change with one to four age, the hair of base portion class floweriness shape also appears at the 8th uromere, at 3 to 8 uromeres, two at every joint, 1,2 liang of uromere side edge region is without this type hair.Escutcheon appears, and the wing bud extends the middle part of the 4th visible joint.
Embodiment 7
By the feeler of above-described embodiment observation nymph in 5 length of time and adult, can find to exist between nymph and the adult very big-difference.Adult feeler 5 joints are black yellow alternate.And nymph feeler in 1 to 5 age is 4 joints, and brown is with increasing the length of time and deepen gradually, each save the end and the base portion color also more shallow, but aberration is not obvious, the black Huang of unlikely formation is met each other.
Find by the observation under optical microscope, 1 age nymph pronotary lateral margin the smooth of the edge; 2 age nymph begin to have the zigzag microspike; 3 age nymph serration more obvious, more sharp-pointed thorn-like thrust is arranged; During to 4 age, the serration degree than 3 age nymph little; And to 5 ages, the pronotary lateral margin serration of nymph disappears, the smooth of the edge.Adult with 5 age nymph the same, do not change, the pronotary lateral margin of polypide is smooth, does not have thrust.
Spot must stinkbug each in age nymph and the uromere outside of belly of adult, can find to have the hair of special base portion class floweriness shape.1 age nymph 3 to 7 uromere outside of belly lateral margins, the hair of special base portion class floweriness shape is arranged, every joint only has 1; Two age nymph also at 3 to 7 uromere outside of belly lateral margins, but every joint has 2; Tritonymph with two age nymph identical, at 3 to 7 uromeres, 2 at every joint; Four age nymph still with two age nymph identical, at 3 to 7 uromeres, 2 at every joint; Five age nymph at 3 to 8 uromeres, two at every joint, difference be the 8th uromere also occur base portion class floweriness shape the hair.
Spot must stinkbug each in age the nymph configuration identical with adult, circle slightly, there is black spots the every joint of belly back side central authorities and both sides, on the abdominal terga living all types of maos.The adult wing covers the belly back side, and the abdominal terga part that wing covers is black all, does not have the black patch, and living without hair.Cause this causes of change, may cover belly back of the body side because the wing development of adult is complete, cause the derivant on the abdominal terga to disappear, concrete mechanism is also indefinite, also needs further research.
Observation post do two five age nymph slide, five age nymph the venter side edge region the hair of the base portion class floweriness shape of giving birth to, have one at 3 to 8 uromeres, every joint has 4.Another is at 3 to 8 uromeres, two at every joint.The reason that causes this species diversity might be male and female difference, also might be sudden change.Concrete reason it be unclear that, and needs research more individual, to sum up reason.
Each, there was the intensive hair together of cluster at the front foot leg section middle part of nymph and adult in age.According to the document of present reading, having no other kinds of report has this feature, whether can be used as the feature that spot palpus stinkbug and other kinds are distinguished, and also needs further research.
By analyzing the feeler that can sum up spot palpus stinkbug adult and nymph very big-difference is arranged.At first, joint number is different, and adult is 5 joints, is 4 joints 5 length of times of nymph.Secondly, on the color, adult is obviously visible black yellow alternate, and nymph 5 the length of time feeler aberration not obvious, form black yellow alternate shape.
According to analyzing the body surface architectural feature variation tendency of finding spot palpus stinkbug nymph pronotary in 5 length of time certain rule is arranged.The pronotary lateral margin has obvious serration 3 ages by smooth the changing in 1 age, again becomes smooth to 5 ages.
In the analysis relatively to the belly feature, can find spot must stinkbug each in age nymph and the venter of adult, the hair of the base portion class floweriness shape of giving birth in the uromere side edge region has the increase trend of quantity.The hair of this special base portion class floweriness shape, when an age, only in the venter side edge region of uromere 3 to 7 joints, every saving given birth to 1; Two age to four the age grade section, 3 to 7 the joint, every saving given birth to 2; During five ages, the 8th uromere also give birth to 2, namely at 3 to 8 joints, every saving given birth to 2; Adult with five age nymph identical, 3 to 8 the joint, every saving given birth to 2.Each in age nymph and adult pronotary on elongated pubescence is arranged, can be used as spot must stinkbug and a feature of other kinds differentiation.
Claims (4)
1. a method of observing spot palpus stinkbug comprises the steps:
(1) spot must be soaked in the stinkbug polypide alcohol;
The spot that (2) will be soaked in the alcohol must take out by the stinkbug sample, and microscopy under anatomical lens is made slide;
(3) the sample slide of making is placed the optical microphotograph Microscopic observation.
2. a kind of method of observing spot palpus stinkbug according to claim 1 is characterized in that, described making slide comprises the steps:
(1) each several part of the integral body of nymph or adult being put into 10%～15% sodium hydroxide solution soaked 5～8 hours;
(2) use the clear water soaking and washing until clean with the intrinsic sewage exchange of mark;
(3) with coloring agent dyeing 2～3 minutes, take out sample and wash out unnecessary coloring agent, put into microslide;
(4) with 30%, 50%, 70%, 85%, 95%, the alcohol of 100% 6 kind of variable concentrations dewaters the whole appearance while dewatering step by step;
(5) drip an amount of dimethylbenzene transparent, with thieving paper blot clean after, drip neutral resins, the cover glass mounting.
3. a kind of method of observing spot palpus stinkbug according to claim 2 is characterized in that, the each several part of adult refers to pronotary, messcutellum, abdominal terga, the belly web of adult in the described step (1).
4. a kind of method of observing spot must stinkbug according to claim 3, it is characterized in that, the each several part of adult be first with adult the head, wing and the foot all take off, with scissors or blade the both side edges of adult polypide is cut, pull open gently backboard and web with tweezers, internal organ and muscle are all removed, cleaned out, again the periproct connecting place is cut off to obtain.
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| CN103947609A (en) * | 2014-04-18 | 2014-07-30 | 青岛农业大学 | Method for observing wax gland orifices of eriosoma lanigerum |
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